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Nondestructive assessment of water content and water stress in plants is an important component in the rational use of crop irrigation management in precision agriculture. Spectral measurements of light reflectance in the UV/VIS/NIR region (350–1075 nm) from individual leaves were acquired under a rapid dehydration protocol for validation of the remote sensing water content assessment in soybean plants. Four gravimetrical approaches of leaf water content assessment were used: relative water content (RWC), foliar water content as percent of total fresh mass (FWCt), foliar water content as percent of dry mass (FWCd), and equivalent water thickness (EWT). Leaf desiccation resulted in changes in optical properties with increasing relative reflectance at wavelengths between 580 and 700 nm. The highest positive correlations were observed for the relations between the photochemical reflectance index (PRI) and EWT (rP = 0.860). Data analysis revealed that the specific water absorption band at 970 nm showed relatively weaker sensitivity to water content parameters. The prediction of leaf water content parameters from PRI measurements was better with RMSEs of 12.4% (rP = 0.786), 9.1% (rP = 0.736), and 0.002 (rP = 0.860) for RWC, FWCt, and EWT (p < 0.001), respectively. The results may contribute to more efficient crop water management and confirmed that EWT has a statistically closer relationship with reflectance indices than other monitored water parameters.

IntroductionSoil health is vital for ecosystems, but excessive use of fertilizers, chemicals, and irrigation harms soil fertility, leading to reduced yields and degradation. Thus, exploring innovative land arrangements and nutrient management for staple crops such as wheat is essential. Organic farming offers a promising solution. This study hypothesized that an appropriate land arrangement, combined with split applications of farmyard manure (FYM) and liquid organic fertilizer, can enhance wheat productivity while also improving soil health. The objective of this study was to investigate the effects of different sowing methods and organic nutrient management practices on the productivity of wheat and soil health.MethodsThis study aimed to examine the impact of sowing methods and nutrient management practices on soil health and organic wheat productivity during the winter seasons of 2021–22 and 2022–23 at the Organic Farming Unit (Agronomy), Rajasthan College of Agriculture, MPUAT, Udaipur. The experiment was arranged in a split-plot design, with three land arrangements/sowing methods in the main plot and eight nutrient management approaches in the subplots.ResultsConcerning different land arrangements, the furrow irrigated raised bed sowing (FIRB) method resulted in the maximum wheat yield (4.34 t ha−1) compared with flat row sowing and zero tillage sowing. With respect to nutrient management practices, 75% of the recommended dose of N (RDN) through the FYM was basal + 25% RDN with the 1st irrigation + Jeevamrut application at 500 L ha−1 during sowing, and the 1st irrigation + Panchagavya spraying at 5% during the booting stage resulted in the highest grain yield (4.47 t ha−1). Both the zero tillage and FIRB land arrangements resulted in better soil biological activities and microbial counts than did flat sowing. The results indicate that sowing wheat via the FIRB method, with the recommended nitrogen dose applied through split applications of FYM along with liquid organic manures such as Jeevamrut and Panchagavya, can be an excellent option for organic wheat cultivation. This approach can enhance both yield and soil health.ConclusionSeventy-five percent RDN through FYM as a basal + 25% RDN during the 1st irrigation + Jeevamrut at 500 L ha−1 during sowing and the 1st irrigation + spraying of Panchagavya at 5% during the booting stage effectively increased the growth and yield of organic wheat. With respect to land arrangements, both FIRB and zero tillage resulted in better growth, yield, and soil biological properties. The practical utility of this study is the optimization of split applications of FYM and land arrangements for organic wheat cultivation.

Drought is characterised as a recurring climatic phenomenon with prolonged duration, affecting land through below-average rainfall and often accompanied by high temperatures. When the available water falls below the optimum level, water deficit or water stress arises, disrupting normal plant processes. This condition poses challenges for plant growth and development as it hampers the internal water transport, induces stomatal closure, and limits access to photosynthetic resources. The study employed the annual sunflower as the experimental plant. The plants were cultivated in a controlled environment with a temperature ranging from 20 to 25°C and a humidity level of 55 to 60%, supplemented by MARS HYDRO artificial LED lighting set to a 12-h photoperiod. Radial changes in the plant stems were monitored using a DD-S type dendrometric sensor to measure radial fluctuations. The collected data were recorded in a dendrometric data logger DL 18. Data collection occurred at hourly intervals from February 20 to March 9, 2023. The nine plants were divided into three groups, each comprising three plants. All plants from groups 1 and 2 received irrigation at one- day intervals (group 1 – 80 cm 3 per plant, group 2 – 40 cm 3 per plant) and group 3 was not irrigated. Based on these findings, visible water stress was evident in the plants under experimental conditions. Consequently, continuous monitoring throughout the growing season will be essential to adjust the irrigation rate to meet the requirements of the plants.

The impact of integrated nutrient management seems crucial for the sustainability of crop production as revealed by studies on long-term experiments. It provided the opportunity to monitor long-term variations in crop yields and associated factors. The impacts of various nutrient management strategies on yields and soil attributes in a rice-wheat system have been researched under a long-term experiment that has been running since 1983 at Punjab Agricultural University, Ludhiana. Further, a positive correlation has been observed between crop yields and soil properties such as soil organic carbon (SOC), nitrogen (N), phosphorus (P), potassium (K) and zinc (Zn). The negative correlation with K could be attributed to soil becoming deficient in K and necessitating the application of potassium fertilizer. The treatments receiving organic manures (green manure, farmyard manure and wheat cut straw) showed a better population of soil microorganisms in comparison to the treatments receiving chemical fertilizers, thereby proving as precursors of sustaining soil health. The best soil characteristics (water-soluble aggregates, exchangeable and non-exchangeable K, fixed and total K) after rice and wheat harvesting were found where 50 % of the recommended NPK was supplemented with farmyard manure (FYM). The build-up of trace elements particularly for Fe and Zn was also noticed. In crystalline Fe oxide bound fraction (CFeOX), Fe increased between 717.1 and 984.8 mg kg−1, while Zn increased between 2.64 and 3.08 mg kg−1. Furthermore, amorphous iron oxide (AFeOX), CFeOX, carbonate (CARB), organic matter (OM) bound and exchangeable (EXCH) Fe and Zn were higher in treatments where organic manures were supplemented with 50 and 25 % N. Farmyard manure showed an incremental trend, followed by wheat cut straw and green manure (GM). The incremental trend in soil quality was noticed with FYM followed by wheat cut straw and GM.

IntroductionThe yield of chickpea is severely hampered by infection wilt caused by several races of Fusarium oxysporum f. sp. ciceris (Foc).MethodsTo understand the underlying molecular mechanisms of resistance against Foc4 Fusarium wilt, RNA sequencing-based shoot transcriptome data of two contrasting chickpea genotypes, namely KWR 108 (resistant) and GL 13001 (susceptible), were generated and analyzed.Results and DiscussionThe shoot transcriptome data showed 1,103 and 1,221 significant DEGs in chickpea genotypes KWR 108 and GL 13001, respectively. Among these, 495 and 608 genes were significantly down and up-regulated in genotypes KWR 108, and 427 and 794 genes were significantly down and up-regulated in genotype GL 13001. The gene ontology (GO) analysis of significant DEGs was performed and the GO of the top 50 DEGs in two contrasting chickpea genotypes showed the highest cellular components as membrane and nucleus, and molecular functions including nucleotide binding, metal ion binding, transferase, kinase, and oxidoreductase activity involved in biological processes such as phosphorylation, oxidation–reduction, cell redox homeostasis process, and DNA repair. Compared to the susceptible genotype which showed significant up-regulation of genes involved in processes like DNA repair, the significantly up-regulated DEGs of the resistant genotypes were involved in processes like energy metabolism and environmental adaptation, particularly host-pathogen interaction. This indicates an efficient utilization of environmental adaptation pathways, energy homeostasis, and stable DNA molecules as the strategy to cope with Fusarium wilt infection in chickpea. The findings of the study will be useful in targeting the genes in designing gene-based markers for association mapping with the traits of interest in chickpea under Fusarium wilt which could be efficiently utilized in marker-assisted breeding of chickpea, particularly against Foc4 Fusarium wilt.

Agricultural productivity is constantly being forced to maintain yield stability to feed the enormously growing world population. However, shrinking arable and nutrient-deprived soil and abiotic and biotic stressor (s) in different magnitudes put additional challenges to achieving global food security. Though well-defined, the concept of macro, micronutrients, and beneficial elements is from a plant nutritional perspective. Among various micronutrients, selenium (Se) is essential in small amounts for the life cycle of organisms, including crops. Selenium has the potential to improve soil health, leading to the improvement of productivity and crop quality. However, Se possesses an immense encouraging phenomenon when supplied within the threshold limit, also having wide variations. The supplementation of Se has exhibited promising outcomes in lessening biotic and abiotic stress in various crops. Besides, bulk form, nano-Se, and biogenic-Se also revealed some merits and limitations. Literature suggests that the possibilities of biogenic-Se in stress alleviation and fortifying foods are encouraging. In this article, apart from adopting a combination of a conventional extensive review of the literature and bibliometric analysis, the authors have assessed the journey of Se in the “soil to spoon” perspective in a diverse agroecosystem to highlight the research gap area. There is no doubt that the time has come to seriously consider the tag of beneficial elements associated with Se, especially in the drastic global climate change era.

Aim of the study: The physiological impact of drought on the crop is reflected into the dendrological changes of the tree or plant body. In this study, we installed automatic dendrometers and we investigated the effect of irrigation dose at different intervals on the growth of sunflower and maize in laboratory conditions. Material and methods: For our research was chosen a sunflower (Helianthus annus L.) and maize (Zea Mays L.) seeded in a laboratory equipped with LED lights Mars Hydro 400 as a substitute source of natural sunlight for 12 hours. Both crops were divided into containers A (irrigation dose of 5 mm was applied each day) and B (the same irrigation dose was applied at 3-day interval). In every container was measured volumetric soil water content with sensors 10HS (Decagon Devices) and dendrometric measurements of plants diameter with sensors DD-S (Ecomatik). Results and conclusions: A comparison of sunflower and maize irrigated at different intervals showed an increase in the diameters of stems at 1-day irrigation compared to 3-day by 0.8 mm (sunflower) and 12.0 mm (maize). The growth of plant mass was continuous at 1-day irrigation with minor fluctuations during the alternation of the dark and light phases. During the 3-day irrigation, greater shrinkage of the stems occurred, indicating the onset of water stress for the crops. The results of the work can be the basic basis for determining the appropriate application of irrigation bag in conditions of changing climate.

The aim of this paper was to design a device for monitoring the work of irrigation technology (in our case, irrigation by sprinkler). Two devices for monitoring selected irrigation operating parameters for two hose reel irrigation machines were designed. During the monitored period of connection of the equipment to the sprinkler, 15 irrigation doses were carried out for both sprinklers. Irrigation operating characteristics working pressure, hose reel speed and selected weather conditions temperature and humidity were monitored. When evaluating the results, we proved the need to monitor the operation of the sprinkler not only by the coefficient of variation Cv, but also by introducing the coefficient of non-uniformity a. The results obtained indicate variability with respect to a particular irrigation dose and the applicable assessment method. The results were reviewed by one-way ANOVA analysis where observed coefficients and irrigation dose were considered as dependence factors. The results indicate a statistically significant impact of the applied quality coefficient of work and thus the impact of a particular device (p < 0.05, Fcrit = 2.77). When evaluating the effect of the included irrigation dose, we also showed a statistically significant effect in both facilities (p < 0.05, F = 1.92). By checking the operation of the hose reel irrigation machine, we managed to successfully apply the proposed classifications, which also perform the function of fault prediction. The proposed facilities show that proper plant operation and timely response can help create more efficient and sustainable irrigation services, not only saving water but also reducing costs for the owner.

Changes in the distribution of annual rainfall totals, together with the increase in temperature over the last 40 years, are causing more frequent periods of drought, and plants are more often exposed to water stress. The aim of this study was to monitor the effect of different water regimes (irrigated and non-irrigated) of individuals of walnut tree (Juglans regia L.) in a private orchard located in the West of Slovakia. Our research was focused on dendrometric and sap flow measurements in the period from 28 March to 2 June 2019. The results showed differences in the sap flow of walnut trees during the budbreak period: when trees were irrigated, sap flow in the diurnal cycle was around 130 g·h−1 (20.48%), higher than in the non-irrigated treatment. Dendrometric differences between the irrigated and non-irrigated treatments were not significant. The sap flow data in the flowering period of the irrigated variant were slightly higher at 150 g·h−1 (35.62%) than non-irrigated. Dendrometric differences were more significant when the difference between the variants was more than 1.5 mm. Continuation of this research and analysis of the data obtained in the coming years will allow us to evaluate the effects of the environment on fruit trees in the long term.

Abstract Mungbean [Vigna radiata L. (Wilczek)] is considered as an extremely nutritious crop possessing a high level of micronutrients, but their low bioavailability in the crop leads to micronutrient malnutrition in humans. Therefore, the present study was conducted to investigate the potential of nutrients viz. boron (B), zinc (Zn) and iron (Fe) biofortification on productivity, nutrient concentration and uptake as well as the economics of mungbean cultivation. In the experiment, the various combinations of RDF with ZnSO4.7H2O (0.5%), FeSO4.7H2O (0.5%) and borax (0.1%) were applied to mungbean variety ML 2056. The combined foliar application of Zn, Fe and B was highly efficient in increasing the yield of grain as well as straw in mungbean exhibiting maximum values i.e. 944 kg ha−1 and 6133 kg ha−1, respectively. Similar results for B, Zn and Fe concentration in grain (27.3 mg kg−1, 35.7 mg kg−1 and 187.1 mg kg−1, respectively) and straw (21.1 mg kg−1, 18.6 mg kg−1 and 376.1 mg kg−1, respectively) of mungbean were observed. Also, uptake of Zn and Fe by grain (31.3 g ha−1 and 164.4 g ha−1, respectively), as well as straw (113.7 g ha−1 and 2295.0 g ha−1, respectively), was maximum for the above treatment. Whereas, the B uptake was found to enhance significantly through the combined application of B, Zn and Fe, where the values 24.0 g ha−1 and 128.7 g ha−1 corresponded to grain and straw, respectively. Thus, combined use of ZnSO4.7H2O (0.5%) + FeSO4.7H2O (0.5%) and borax (0.1%) significantly improved the yield outcomes, the concentration of B, Zn and Fe, uptake and economic returns of mungbean cultivation to alleviate the B, Zn and Fe deficiency.

Crop failure is largely caused by various climate hazards, and among them, heat stress is the primary factor hindering crop production. The significant global loss of crop yield is primarily due to heat-related damage during the reproductive phase. Terminal heat stress has been well documented in wheat, causing morphophysiological alterations, biochemical disruptions, and reduction of genetic potential. The formation of shoots and roots, the effect on the double ridge stage, and early biomass in the vegetative stage are also impacted by heat stress. The final negative outcomes of heat stress include reduced grain number and weight, slower grain filling rate, reduced grain quality, and shorter grain filling duration. Plants have developed mechanisms to adapt to heat stress through modifications in their morphological or growth responses, physiological and biochemical pathways, and changes in enzyme reactions. Numerous heat tolerance genes have been identified in wheat, but the more extensive study is needed to increase heat tolerance in crops to satisfy the food demands of the world’s growing population. The global food policy needs to prioritize and promote additional joint research and the development of heat-tolerant wheat breeding to ensure the world’s food security.

Environmental stresses such as drought, high salinity, and low temperature can adversely modulate the field crop’s ability by altering the morphological, physiological, and biochemical processes of the plants. It is estimated that about 50% + of the productivity of several crops is limited due to various types of abiotic stresses either presence alone or in combination (s). However, there are two ways plants can survive against these abiotic stresses; a) through management practices and b) through adaptive mechanisms to tolerate plants. These adaptive mechanisms of tolerant plants are mostly linked to their signalling transduction pathway, triggering the action of plant transcription factors and controlling the expression of various stress-regulated genes. In recent times, several studies found that Zn-finger motifs have a significant function during abiotic stress response in plants. In the first report, a wide range of Zn-binding motifs has been recognized and termed Zn-fingers. Since the zinc finger motifs regulate the function of stress-responsive genes. The Zn-finger was first reported as a repeated Zn-binding motif, comprising conserved cysteine (Cys) and histidine (His) ligands, in Xenopus laevis oocytes as a transcription factor (TF) IIIA (or TFIIIA). In the proteins where Zn2+ is mainly attached to amino acid residues and thus espousing a tetrahedral coordination geometry. The physical nature of Zn-proteins, defining the attraction of Zn-proteins for Zn2+, is crucial for having an in-depth knowledge of how a Zn2+ facilitates their characteristic function and how proteins control its mobility (intra and intercellular) as well as cellular availability. The current review summarized the concept, importance and mechanisms of Zn-finger motifs during abiotic stress response in plants.

In arid and semiarid regions and under rainfed conditions, water availability is one of the principal ecological constraints that hinder agriculture’s sustainability. The super absorbent polymer (agricultural) is water-absorbing and is cross-linked to absorb aqueous solutions through bonding with water molecules. It is a new approach to water management under water-stressed conditions to conserve soil moisture in the active rooting zone of crops by reducing the evaporation, deep percolation, and runoff losses. Agricultural hydrogels are water retention granules which swell their original size to numerous intervals when they come in contact with water. It can absorb and retain a huge amount of moisture under plentiful rainfall and irrigation events and release it back to the soil for mitigating crop water demand when the rhizosphere zone dries up under drought conditions. It plays multifarious roles in agriculture including soil-water retainer, nutrient and pesticide carriers, seed coating, soil erosion reducer, and food additives. It has the extraordinary ability in improving different physicochemical, hydrophysical, and biological properties of soil, simultaneously decreasing irrigation frequency, enhancing the water and nutrient use efficiencies, and increasing the yield and quality of the field, plantation, ornamental, and vegetable crops. These biodegradable materials are nontoxic to the soil, crop, and environment. Hence, the addition of the hydrogel polymer will be a promising and feasible technological tool for augmenting crop productivity under moisture stressed conditions.

Globally, many developing countries are facing silent epidemics of nutritional deficiencies in human beings and animals. The lack of diversity in diet, i.e., cereal-based crops deficient in mineral nutrients is an additional threat to nutritional quality. The present review accounts for the significance of biofortification as a process to enhance the productivity of crops and also an agricultural solution to address the issues of nutritional security. In this endeavor, different innovative and specific biofortification approaches have been discussed for nutrient enrichment of field crops including cereals, pulses, oilseeds and fodder crops. The agronomic approach increases the micronutrient density in crops with soil and foliar application of fertilizers including amendments. The biofortification through conventional breeding approach includes the selection of efficient genotypes, practicing crossing of plants with desirable nutritional traits without sacrificing agricultural and economic productivity. However, the transgenic/biotechnological approach involves the synthesis of transgenes for micronutrient re-translocation between tissues to enhance their bioavailability. Soil microorganisms enhance nutrient content in the rhizosphere through diverse mechanisms such as synthesis, mobilization, transformations and siderophore production which accumulate more minerals in plants. Different sources of micronutrients viz. mineral solutions, chelates and nanoparticles play a pivotal role in the process of biofortification as it regulates the absorption rates and mechanisms in plants. Apart from the quality parameters, biofortification also improved the crop yield to alleviate hidden hunger thus proving to be a sustainable and cost-effective approach. Thus, this review article conveys a message for researchers about the adequate potential of biofortification to increase crop productivity and nourish the crop with additional nutrient content to provide food security and nutritional quality to humans and livestock.

Agricultural sustainability is of foremost importance for maintaining high food production. Irresponsible resource use not only negatively affects agroecology, but also reduces the economic profitability of the production system. Among different resources, soil is one of the most vital resources of agriculture. Soil fertility is the key to achieve high crop productivity. Maintaining soil fertility and soil health requires conscious management effort to avoid excessive nutrient loss, sustain organic carbon content, and minimize soil contamination. Though the use of chemical fertilizers have successfully improved crop production, its integration with organic manures and other bioinoculants helps in improving nutrient use efficiency, improves soil health and to some extent ameliorates some of the constraints associated with excessive fertilizer application. In addition to nutrient supplementation, bioinoculants have other beneficial effects such as plant growth-promoting activity, nutrient mobilization and solubilization, soil decontamination and/or detoxification, etc. During the present time, high energy based chemical inputs also caused havoc to agriculture because of the ill effects of global warming and climate change. Under the consequences of climate change, the use of bioinputs may be considered as a suitable mitigation option. Bioinoculants, as a concept, is not something new to agricultural science, however; it is one of the areas where consistent innovations have been made. Understanding the role of bioinoculants, the scope of their use, and analysing their performance in various environments are key to the successful adaptation of this technology in agriculture.

To assess the reliability and sensitivity of non-invasive optical methods to detect the early effects of water deficit in the field, we analyzed the time-series of non-invasive measurements obtained in a dry season in a representative collection of wheat genotypes grown in small-plot field trials, in non-irrigated and irrigated variants. Despite a progressive water deficit and significant yield loss, the measurements indicated very minor changes in chlorophyll content or canopy cover. This corresponded well to the insignificant differences in spectral reflectance normalized difference vegetation index (NDVI) values. On the other hand, we identified the significant and rapid response of fast fluorescence kinetics data following the onset of irrigation. Analysis of parameters showed the main effects of drought were associated with changes in the amplitude of the I–P phase of the OJIP transient, indicating changes at the level of photosystem I and beyond. Statistical analyses identified the integrative parameter performance index PItot as the most sensitive parameter, which well-reflects the differences in responses of the genotypes to water deficit. Our results suggest that focusing on photosynthetic functions detected by the rapid chlorophyll fluorescence records can provide more accurate information on the drought stress level, compared to the structural data obtained by absorbance or reflectance measurements.