557 results on '"Vilar M"'
Search Results
2. Design of Multiplexing CRISPR/Cas9 Constructs for Plant Genome Engineering Using the GoldenBraid DNA Assembly Standard
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Vazquez-Vilar, M., primary, Juarez, P., additional, Bernabé-Orts, J. M., additional, and Orzaez, D., additional
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- 2022
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3. Neurotrophins and Neurotrophin Receptors
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Franco, M.L., primary, Comaposada-Baró, R., additional, and Vilar, M., additional
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- 2020
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4. Chitosan-Ag-TiO2 films: An effective photocatalyst under visible light
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Jbeli, A., Hamden, Z., Bouattour, S., Ferraria, A.M., Conceição, D.S., Ferreira, L.F. Vieira, Chehimi, M.M., do Rego, A.M. Botelho, Rei Vilar, M., and Boufi, S.
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- 2018
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5. Topic: AS07-Singular Entities/Subtypes/AS07a-ARCH, CCUS, ICUS: CLINICAL AND CARDIOVASCULAR RISK ASSESSMENT OF SUBJECTS WITH JAK2-V617F CLONAL HEMATOPOIESIS
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Garrido, S., primary, López-Cadenas, F., additional, García-Ávila, S., additional, Tazón-Vega, B., additional, Blanco, A., additional, Palomo, L., additional, Piris-Villaespesa, M., additional, García-Gutiérrez, V., additional, De Vilar, M., additional, Sola, M., additional, Yordi, A. Molero, additional, González, A. Pérez, additional, Restrepo, J., additional, Fox, L., additional, Saumell, S., additional, Novoa, S., additional, Gallur, L., additional, Salamero, O., additional, Oristrell, G., additional, Castellví, J., additional, Valcárcel, D., additional, and Montoro, M.J., additional
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- 2023
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6. Beyond genetics: Deciphering the impact of missense variants in CAD deficiency.
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Caño-Ochoa, F. Del, Ng, B.G., Rubio-Del-Campo, A., Mahajan, S., Wilson, M.P., Vilar, M., Rymen, D., Sánchez-Pintos, P., Kenny, J., Ley Martos, M., Campos, T., Wortmann, S.B., Freeze, H.H., Ramón-Maiques, S., Caño-Ochoa, F. Del, Ng, B.G., Rubio-Del-Campo, A., Mahajan, S., Wilson, M.P., Vilar, M., Rymen, D., Sánchez-Pintos, P., Kenny, J., Ley Martos, M., Campos, T., Wortmann, S.B., Freeze, H.H., and Ramón-Maiques, S.
- Abstract
Contains fulltext : 299849.pdf (Publisher’s version ) (Open Access), CAD is a large, 2225 amino acid multienzymatic protein required for de novo pyrimidine biosynthesis. Pathological CAD variants cause a developmental and epileptic encephalopathy which is highly responsive to uridine supplements. CAD deficiency is difficult to diagnose because symptoms are nonspecific, there is no biomarker, and the protein has over 1000 known variants. To improve diagnosis, we assessed the pathogenicity of 20 unreported missense CAD variants using a growth complementation assay that identified 11 pathogenic variants in seven affected individuals; they would benefit from uridine treatment. We also tested nine variants previously reported as pathogenic and confirmed the damaging effect of seven. However, we reclassified two variants as likely benign based on our assay, which is consistent with their long-term follow-up with uridine. We found that several computational methods are unreliable predictors of pathogenic CAD variants, so we extended the functional assay results by studying the impact of pathogenic variants at the protein level. We focused on CAD's dihydroorotase (DHO) domain because it accumulates the largest density of damaging missense changes. The atomic-resolution structures of eight DHO pathogenic variants, combined with functional and molecular dynamics analyses, provided a comprehensive structural and functional understanding of the activity, stability, and oligomerization of CAD's DHO domain. Combining our functional and protein structural analysis can help refine clinical diagnostic workflow for CAD variants in the genomics era., 01 november 2023
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- 2023
7. Comparative analysis of the Squamosa Promoter Binding-Like (SPL) gene family in Nicotiana benthamiana and Nicotiana tabacum
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De Paola, C, Garcia-Carpintero, V, Vazquez-Vilar, M, Kaminski, K, Fernandez-del-Carmen, A, Sierro, N, Ivanov, N.V., Giuliano, G., Waterhouse, P., Orzáez Calatayud, Diego, De Paola, C, Garcia-Carpintero, V, Vazquez-Vilar, M, Kaminski, K, Fernandez-del-Carmen, A, Sierro, N, Ivanov, N.V., Giuliano, G., Waterhouse, P., and Orzáez Calatayud, Diego
- Abstract
SQUAMOSA PROMOTER BINDING-LIKE (SPL) proteins constitute a large family of transcription factors known to play key roles in growth and developmental processes, including juvenile-to-adult and vegetative-to-reproductive phase transitions. This makes SPLs interesting targets for precision breeding in plants of the Nicotiana genus used as e.g. recombinant biofactories. We report the identification of 49 SPL genes in Nicotiana tabacum cv. K326 and 43 SPL genes in Nicotiana benthamiana LAB strain, which were classified into eight phylogenetic groups according to the SPL classification in Arabidopsis. Exon-intron gene structure and DNA-binding domains were highly conserved between homeologues and orthologues. Thirty of the NbSPL genes and 33 of the NtSPL genes were found to be possible targets of microRNA 156. The expression of SPL genes in leaves was analysed by RNA-seq at three different stages, revealing that genes not under miR156 control were in general constitutively expressed at high levels, whereas miR156-regulated genes showed lower expression, often developmentally regulated. We selected the N. benthamiana SPL13_1a gene as target for a CRISPR/Cas9 knock-out experiment. We show here that a full knock-out in this single gene leads to a significant delay in flowering time, a trait that could be exploited to increase biomass for recombinant protein production.
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- 2023
8. Shipyards of the 21st century: Industrial internet of things on site
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Diaz-Casas, Vicente, primary, Munin Doce, Alicia, additional, Trueba Martinez, Pedro, additional, Ferreno Gonzalez, Sara, additional, and Vilar, M., additional
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- 2018
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9. Is optimal always optimal? Chronotype, time-of-day, and children's cognitive performance in remote neuropsychological assessment
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Bettencourt, C., primary, Pires, L., additional, Almeida, F., additional, Vilar, M., additional, Cruz, H., additional, Leitão, J.A., additional, and Gomes, A. Allen, additional
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- 2022
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10. P105 - Topic: AS07-Singular Entities/Subtypes/AS07a-ARCH, CCUS, ICUS: CLINICAL AND CARDIOVASCULAR RISK ASSESSMENT OF SUBJECTS WITH JAK2-V617F CLONAL HEMATOPOIESIS
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Garrido, S., López-Cadenas, F., García-Ávila, S., Tazón-Vega, B., Blanco, A., Palomo, L., Piris-Villaespesa, M., García-Gutiérrez, V., De Vilar, M., Sola, M., Yordi, A. Molero, González, A. Pérez, Restrepo, J., Fox, L., Saumell, S., Novoa, S., Gallur, L., Salamero, O., Oristrell, G., Castellví, J., Valcárcel, D., and Montoro, M.J.
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- 2023
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11. Optimizing Water Quality in a River Section
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Vilar, M. A., Alvarez-Vázquez, L. J., Martínez, A., Vázquez-Méndez, M. E., Constanda, Christian, editor, and Harris, Paul J., editor
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- 2011
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12. Some Contributions About an Implicit Discretization of a 1D Inviscid Model for River Flows
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de Castro, A. Bermúdez, Muñoz-Sola, R., Rodríguez, C., Vilar, M. Ángel, Benzoni-Gavage, Sylvie, editor, and Serre, Denis, editor
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- 2008
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13. Fishways Design: An Application of the Optimal Control Theory
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Alvarez-Vázquez, L. J., Martínez, A., Vázquez-Méndez, M. E., Vilar, M. A., Kunisch, Karl, editor, Of, Günther, editor, and Steinbach, Olaf, editor
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- 2008
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14. Friedrich Hölderlin: Antología. Versiones y probaturas de Joan Vinyoli, edición de Tobias Christ y Anacleto Ferrer, spanisch und deutsch, (Visor de Poesía 921) Madrid: Visor Libros 2015, 184 S.
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Loreto Vilar, M., primary
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- 2017
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15. Structural Characterization of the p75 Neurotrophin Receptor
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Vilar, M., primary
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- 2017
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16. Finite elements numerical solution to deep beams based on layerwise displacement field
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Vilar, M. M. S., Sartorato, M., Santana, H. B., and Leite, M. R.
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- 2018
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17. Mathematical Modelling and Numerical Optimization in the Process of River Pollution Control
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Alvarez-Vázquez, L.J., Martínez, A., Vázquez-Méndez, M.E., Vilar, M., de Castro, Alfredo Bermúdez, editor, Gómez, Dolores, editor, Quintela, Peregrina, editor, and Salgado, Pilar, editor
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- 2006
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18. Potato virus X -delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming inNicotiana benthamiana
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Selma, S, primary, Gianoglio, S, additional, Uranga, M, additional, Vázquez-Vilar, M, additional, Espinosa-Ruiz, A, additional, Drapal, M, additional, Fraser, PD, additional, Daròs, JA, additional, and Orzaez, D, additional
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- 2022
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19. Crystal structure of SARS-CoV-2 N-CTD
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Ciges-Tomas, J.R., primary and Vilar, M., additional
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- 2022
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20. Crystal Structure of SARS-CoV-2 N-CTD in complex with GTP (II)
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Ciges-Tomas, J.R., primary and Vilar, M., additional
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- 2022
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21. A Cost-Benefit Analysis of COVID-19 Vaccination in Catalonia
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Lopez, F, Catala, M, Prats, C, Estrada, O, Oliva, I, Prat, N, Isnard, M, Valles, R, Vilar, M, Clotet, B, Argimon, JM, Aran, A, and Ara, J
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pharmacoeconomics ,cost-benefit analysis ,COVID-19 ,health economics ,vaccination ,economic appraisal - Abstract
(1) Background: In epidemiological terms, it has been possible to calculate the savings in health resources and the reduction in the health effects of COVID vaccines. Conducting an economic evaluation, some studies have estimated its cost-effectiveness; the vaccination shows highly favorable results, cost-saving in some cases. (2) Methods: Cost-benefit analysis of the vaccination campaign in the North Metropolitan Health Region (Catalonia). An epidemiological model based on observational data and before and after comparison is used. The information on the doses used and the assigned resources (conventional hospital beds, ICU, number of tests) was extracted from administrative data from the largest primary care provider in the region (Catalan Institute of Health). A distinction was made between the social perspective and the health system. (3) Results: the costs of vaccination are estimated at 137 million euros (euro48.05/dose administered). This figure is significantly lower than the positive impacts of the vaccination campaign, which are estimated at 470 million euros (euro164/dose administered). Of these, 18% corresponds to the reduction in ICU discharges, 16% to the reduction in conventional hospital discharges, 5% to the reduction in PCR tests and 1% to the reduction in RAT tests. The monetization of deaths and cases that avoid sequelae account for 53% and 5% of total savings, respectively. The benefit/cost ratio is estimated at 3.4 from a social perspective and 1.4 from a health system perspective. The social benefits of vaccination are estimated at euro116.67 per vaccine dose (euro19.93 from the perspective of the health system). (4) Conclusions: The mass vaccination campaign against COVID is cost-saving. From a social perspective, most of these savings come from the monetization of the reduction in mortality and cases with sequelae, although the intervention is equally widely cost-effective from the health system perspective thanks to the reduction in the use of resources. It is concluded that, from an economic perspective, the vaccination campaign has high social returns.
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- 2022
22. Is optimal always optimal? Chronotype, time-of-day, and children's cognitive performance in remote neuropsychological assessment
- Author
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Bettencourt, C., Pires, L., Almeida, F. G., Vilar, M., Cruz, H, Leitão, J. A., and Gomes, A. Allen
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- 2022
23. Analysis of the spatial variation of Bovine tuberculosis disease risk in Spain (2006–2009)
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Allepuz, A., Casal, J., Napp, S., Saez, M., Alba, A., Vilar, M., Domingo, M., González, M.A., Duran-Ferrer, M., Vicente, J., Álvarez, J., Muñoz, M., and Saez, J.L.
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- 2011
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24. Synthetic biology open language visual (SBOL Visual) version 2.3
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Baig, H, Fontanarossa, P, Kulkarni, V, McLaughlin, J, Vaidyanathan, P, Bartley, B, Bhakta, S, Bhatia, S, Bissell, M, Clancy, K, Cox, RS, Goñi Moreno, A, Gorochowski, T, Grunberg, R, Lee, J, Luna, A, Madsen, C, Misirli, G, Nguyen, T, Le Novere, N, Palchick, Z, Pocock, M, Roehner, N, Sauro, H, Scott-Brown, J, Sexton, JT, Stan, G-B, Tabor, JJ, Terry, L, Vazquez Vilar, M, Voigt, CA, Wipat, A, Zong, D, Zundel, Z, Beal, J, and Myers, C
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R1 - Abstract
People who are engineering biological organisms often find it useful to communicate in diagrams, both about the structure of the nucleic acid sequences that they are engineering and about the functional relationships between sequence features and other molecular species. Some typical practices and conventions have begun to emerge for such diagrams. The Synthetic Biology Open Language Visual (SBOL Visual) has been developed as a standard for organizing and systematizing such conventions in order to produce a coherent language for expressing the structure and function of genetic designs. This document details version 2.3 of SBOL Visual, which builds on the prior SBOL Visual 2.2 in several ways. First, the specification now includes higher-level "interactions with interactions," such as an inducer molecule stimulating a repression interaction. Second, binding with a nucleic acid backbone can be shown by overlapping glyphs, as with other molecular complexes. Finally, a new "unspecified interaction" glyph is added for visualizing interactions whose nature is unknown, the "insulator" glyph is deprecated in favor of a new "inert DNA spacer" glyph, and the polypeptide region glyph is recommended for showing 2A sequences.
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- 2021
25. High-resolution electron energy loss spectroscopy as a probe of SAMs
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Rei Vilar, M. and Botelho do Rego, A.M.
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- 2009
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26. Understanding the relationship between the structure of an organofunctional methoxysilane bilayer and its properties as adhesion primer for Zn-epoxidized rubber system
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Najari, A., Lang, P., Rei Vilar, M., Lacaze, P.C., and Mauer, D.
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- 2009
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27. Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators
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Selma, S, primary, Sanmartín, N, additional, Espinosa-Ruiz, A, additional, Gianoglio, S, additional, Lopez-Gresa, MP, additional, Vázquez-Vilar, M, additional, Flors, V, additional, Granell, A, additional, and Orzaez, D, additional
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- 2021
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28. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gómez, E, Alessandri, C, Ali, M, Alim Al-Bari, MA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Álvarez, ÉMC, Alves, S, Alves da Costa, C, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, Z, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Antón, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araújo, WL, Araya, J, Arden, C, Arévalo, M-A, Arguelles, S, Arias, E, Arikkath, J, Arimoto, H, Ariosa, AR, Armstrong-James, D, Arnauné-Pelloquin, L, Aroca, A, Arroyo, DS, Arsov, I, Artero, R, Asaro, DML, Aschner, M, Ashrafizadeh, M, Ashur-Fabian, O, Atanasov, AG, Au, AK, Auberger, P, Auner, HW, Aurelian, L, Autelli, R, Avagliano, L, Ávalos, Y, Aveic, S, Aveleira, CA, Avin-Wittenberg, T, Aydin, Y, Ayton, S, Ayyadevara, S, Azzopardi, M, Baba, M, Backer, JM, Backues, SK, Bae, D-H, Bae, O-N, Bae, SH, Baehrecke, EH, Baek, A, Baek, S-H, Baek, SH, Bagetta, G, Bagniewska-Zadworna, A, Bai, H, Bai, J, Bai, X, Bai, Y, Bairagi, N, Baksi, S, Balbi, T, Baldari, CT, Balduini, W, Ballabio, A, Ballester, M, Balazadeh, S, Balzan, R, Bandopadhyay, R, Banerjee, S, Bánréti, Á, Bao, Y, Baptista, MS, Baracca, A, Barbati, C, Bargiela, A, Barilà, D, Barlow, PG, Barmada, SJ, Barreiro, E, Barreto, GE, Bartek, J, Bartel, B, Bartolome, A, Barve, GR, Basagoudanavar, SH, Bassham, DC, Bast, RC, Basu, A, Batoko, H, Batten, I, Baulieu, EE, Baumgarner, BL, Bayry, J, Beale, R, Beau, I, Beaumatin, F, Bechara, LRG, Beck, GR, Beers, MF, Begun, J, Behrends, C, Behrens, GMN, Bei, R, Bejarano, E, Bel, S, Behl, C, Belaid, A, Belgareh-Touzé, N, Bellarosa, C, Belleudi, F, Belló Pérez, M, Bello-Morales, R, Beltran, JSDO, Beltran, S, Benbrook, DM, Bendorius, M, Benitez, BA, Benito-Cuesta, I, Bensalem, J, Berchtold, MW, Berezowska, S, Bergamaschi, D, Bergami, M, Bergmann, A, Berliocchi, L, Berlioz-Torrent, C, Bernard, A, Berthoux, L, Besirli, CG, Besteiro, S, Betin, VM, Beyaert, R, Bezbradica, JS, Bhaskar, K, Bhatia-Kissova, I, Bhattacharya, R, Bhattacharya, S, Bhattacharyya, S, Bhuiyan, MS, Bhutia, SK, Bi, L, Bi, X, Biden, TJ, Bijian, K, Billes, VA, Binart, N, Bincoletto, C, Birgisdottir, AB, Bjorkoy, G, Blanco, G, Blas-Garcia, A, Blasiak, J, Blomgran, R, Blomgren, K, Blum, JS, Boada-Romero, E, Boban, M, Boesze-Battaglia, K, Boeuf, P, Boland, B, Bomont, P, Bonaldo, P, Bonam, SR, Bonfili, L, Bonifacino, JS, Boone, BA, Bootman, MD, Bordi, M, Borner, C, Bornhauser, BC, Borthakur, G, Bosch, J, Bose, S, Botana, LM, Botas, J, Boulanger, CM, Boulton, ME, Bourdenx, M, Bourgeois, B, Bourke, NM, Bousquet, G, Boya, P, Bozhkov, PV, Bozi, LHM, Bozkurt, TO, Brackney, DE, Brandts, CH, Braun, RJ, Braus, GH, Bravo-Sagua, R, Bravo-San Pedro, JM, Brest, P, Bringer, M-A, Briones-Herrera, A, Broaddus, VC, Brodersen, P, Brodsky, JL, Brody, SL, Bronson, PG, Bronstein, JM, Brown, CN, Brown, RE, Brum, PC, Brumell, JH, Brunetti-Pierri, N, Bruno, D, Bryson-Richardson, RJ, Bucci, C, Buchrieser, C, Bueno, M, Buitrago-Molina, LE, Buraschi, S, Buch, S, Buchan, JR, Buckingham, EM, Budak, H, Budini, M, Bultynck, G, Burada, F, Burgoyne, JR, Burón, MI, Bustos, V, Büttner, S, Butturini, E, Byrd, A, Cabas, I, Cabrera-Benitez, S, Cadwell, K, Cai, J, Cai, L, Cai, Q, Cairó, M, Calbet, JA, Caldwell, GA, Caldwell, KA, Call, JA, Calvani, R, Calvo, AC, Calvo-Rubio Barrera, M, Camara, NO, Camonis, JH, Camougrand, N, Campanella, M, Campbell, EM, Campbell-Valois, F-X, Campello, S, Campesi, I, Campos, JC, Camuzard, O, Cancino, J, Candido de Almeida, D, Canesi, L, Caniggia, I, Canonico, B, Cantí, C, Cao, B, Caraglia, M, Caramés, B, Carchman, EH, Cardenal-Muñoz, E, Cardenas, C, Cardenas, L, Cardoso, SM, Carew, JS, Carle, GF, Carleton, G, Carloni, S, Carmona-Gutierrez, D, Carneiro, LA, Carnevali, O, Carosi, JM, Carra, S, Carrier, A, Carrier, L, Carroll, B, Carter, AB, Carvalho, AN, Casanova, M, Casas, C, Casas, J, Cassioli, C, Castillo, EF, Castillo, K, Castillo-Lluva, S, Castoldi, F, Castori, M, Castro, AF, Castro-Caldas, M, Castro-Hernandez, J, Castro-Obregon, S, Catz, SD, Cavadas, C, Cavaliere, F, Cavallini, G, Cavinato, M, Cayuela, ML, Cebollada Rica, P, Cecarini, V, Cecconi, F, Cechowska-Pasko, M, Cenci, S, Ceperuelo-Mallafré, V, Cerqueira, JJ, Cerutti, JM, Cervia, D, Cetintas, VB, Cetrullo, S, Chae, H-J, Chagin, AS, Chai, C-Y, Chakrabarti, G, Chakrabarti, O, Chakraborty, T, Chami, M, Chamilos, G, Chan, DW, Chan, EYW, Chan, ED, Chan, HYE, Chan, HH, Chan, H, Chan, MTV, Chan, YS, Chandra, PK, Chang, C-P, Chang, C, Chang, H-C, Chang, K, Chao, J, Chapman, T, Charlet-Berguerand, N, Chatterjee, S, Chaube, SK, Chaudhary, A, Chauhan, S, Chaum, E, Checler, F, Cheetham, ME, Chen, C-S, Chen, G-C, Chen, J-F, Chen, LL, Chen, L, Chen, M, Chen, M-K, Chen, N, Chen, Q, Chen, R-H, Chen, S, Chen, W, Chen, X-M, Chen, X-W, Chen, X, Chen, Y, Chen, Y-G, Chen, Y-J, Chen, Y-Q, Chen, ZS, Chen, Z, Chen, Z-H, Chen, ZJ, Cheng, H, Cheng, J, Cheng, S-Y, Cheng, W, Cheng, X, Cheng, X-T, Cheng, Y, Cheng, Z, Cheong, H, Cheong, JK, Chernyak, BV, Cherry, S, Cheung, CFR, Cheung, CHA, Cheung, K-H, Chevet, E, Chi, RJ, Chiang, AKS, Chiaradonna, F, Chiarelli, R, Chiariello, M, Chica, N, Chiocca, S, Chiong, M, Chiou, S-H, Chiramel, AI, Chiurchiù, V, Cho, D-H, Choe, S-K, Choi, AMK, Choi, ME, Choudhury, KR, Chow, NS, Chu, CT, Chua, JP, Chua, JJE, Chung, H, Chung, KP, Chung, S, Chung, S-H, Chung, Y-L, Cianfanelli, V, Ciechomska, IA, Cifuentes, M, Cinque, L, Cirak, S, Cirone, M, Clague, MJ, Clarke, R, Clementi, E, Coccia, EM, Codogno, P, Cohen, E, Cohen, MM, Colasanti, T, Colasuonno, F, Colbert, RA, Colell, A, Čolić, M, Coll, NS, Collins, MO, Colombo, MI, Colón-Ramos, DA, Combaret, L, Comincini, S, Cominetti, MR, Consiglio, A, Conte, A, Conti, F, Contu, VR, Cookson, MR, Coombs, KM, Coppens, I, Corasaniti, MT, Corkery, DP, Cordes, N, Cortese, K, Costa, MDC, Costantino, S, Costelli, P, Coto-Montes, A, Crack, PJ, Crespo, JL, Criollo, A, Crippa, V, Cristofani, R, Csizmadia, T, Cuadrado, A, Cui, B, Cui, J, Cui, Y, Culetto, E, Cumino, AC, Cybulsky, AV, Czaja, MJ, Czuczwar, SJ, D'Adamo, S, D'Amelio, M, D'Arcangelo, D, D'Lugos, AC, D'Orazi, G, da Silva, JA, Dafsari, HS, Dagda, RK, Dagdas, Y, Daglia, M, Dai, X, Dai, Y, Dal Col, J, Dalhaimer, P, Dalla Valle, L, Dallenga, T, Dalmasso, G, Damme, M, Dando, I, Dantuma, NP, Darling, AL, Das, H, Dasarathy, S, Dasari, SK, Dash, S, Daumke, O, Dauphinee, AN, Davies, JS, Dávila, VA, Davis, RJ, 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- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
- Published
- 2021
29. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).
- Author
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Quadrilatero J., Walker D.W., Walker M.J., Walker S.A., Walter J., Wandosell F.G., Wang B., Wang C., Wang C.-Y., Wang D., Wang F., Wang G., Wang H., Wang H.-G., Wang J., Wang K., Wang L., Wang M.H., Wang M., Wang N., Wang P., Wang Q.J., Wang Q., Wang Q.K., Wang Q.A., Wang W.-T., Wang W., Wang X., Wang Y.-Y., Wang Y., Wang Z., Warnes G., Warnsmann V., Watada H., Watanabe E., Watchon M., Wawrzynska A., Weaver T.E., Wegrzyn G., Wehman A.M., Wei H., Wei L., Wei T., Wei Y., Weiergraber O.H., Weihl C.C., Weindl G., Weiskirchen R., Wells A., Wen R.H., Wen X., Werner A., Weykopf B., Wheatley S.P., W... [Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., Anton Z., Anwar T., Apetoh L., Apostolova N., Araki T., Araki Y., Arasaki K., Araujo W.L., Araya J., Arden C., Arevalo M.-A., Arguelles S., Arias E., Arikkath J., Arimoto H., Ariosa A.R., Armstrong-James D., Arnaune-Pelloquin L., Aroca A., Arroyo D.S., Arsov I., Artero R., Asaro D.M.L., Aschner M., Ashrafizadeh M., Ashur-Fabian O., Atanasov A.G., Au A.K., Auberger P., Auner H.W., Aurelian L., Autelli R., Avagliano L., Avalos Y., Aveic S., Aveleira C.A., Avin-Wittenberg T., Aydin Y., Ayton S., Ayyadevara S., Azzopardi M., Baba M., Backer J.M., Backues S.K., Bae D.-H., Bae O.-N., Bae S.H., Baehrecke E.H., Baek A., Baek S.-H., Baek S.H., Bagetta G., Bagniewska-Zadworna A., Bai H., Bai J., Bai X., Bai Y., Bairagi N., Baksi S., Balbi T., Baldari C.T., Balduini W., Ballabio A., Ballester M., Balazadeh S., Balzan R., Bandopadhyay R., Banerjee S., Banreti A., Bao Y., Baptista M.S., Baracca A., Barbati C., Bargiela A., Barila D., Barlow P.G., Barmada S.J., Barreiro E., Barreto G.E., Bartek J., 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[Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., 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Kroemer G., Ktistakis N.T., Kuchitsu K., Kuenen S., Kuerschner L., Kukar T., Kumar A., Kumar D., Kumar S., Kume S., Kumsta C., Kundu C.N., Kundu M., Kunnumakkara A.B., Kurgan L., Kutateladze T.G., Kutlu O., Kwak S., Kwon H.J., Kwon T.K., Kwon Y.T., Kyrmizi I., La Spada A., Labonte P., Ladoire S., Laface I., Lafont F., Lagace D.C., Lahiri V., Lai Z., Laird A.S., Lakkaraju A., Lamark T., Lan S.-H., Landajuela A., Lane D.J.R., Lane J.D., Lang C.H., Lange C., Langel U., Langer R., Lapaquette P., Laporte J., LaRusso N.F., Lastres-Becker I., Lau W.C.Y., Laurie G.W., Lavandero S., Law B.Y.K., Law H.K.-W., Layfield R., Le W., Le Stunff H., Leary A.Y., Lebrun J.-J., Leck L.Y.W., Leduc-Gaudet J.-P., Lee C., Lee C.-P., Lee D.-H., Lee E.B., Lee E.F., Lee G.M., Lee H.-J., Lee H.K., Lee J.M., Lee J.S., Lee J.-A., Lee J.-Y., Lee J.H., Lee M., Lee M.G., Lee M.J., Lee M.-S., Lee S.-J., Lee S.Y., Lee S.B., Lee W.H., Lee Y.-R., Lee Y.-H., Lee Y., Lefebvre C., Legouis R., Lei Y.L., Lei Y., Leikin S., Leitinger G., Lemus L., Leng S., Lenoir O., Lenz G., Lenz H.J., Lenzi P., Leon Y., Leopoldino A.M., Leschczyk C., Leskela S., Letellier E., Leung C.-T., Leung P.S., Leventhal J.S., Levine B., Lewis P.A., Ley K., Li B., Li D.-Q., Li J., Li K., Li L., Li M., Li P.-L., Li M.-Q., Li Q., Li S., Li T., Li W., Li X., Li Y.-P., Li Y., Lian J., Liang C., Liang Q., Liang W., Liang Y., Liao G., Liao L., Liao M., Liao Y.-F., Librizzi M., Lie P.P.Y., Lilly M.A., Lim H.J., Lima T.R.R., Limana F., Lin C., Lin C.-W., Lin D.-S., Lin F.-C., Lin J.D., Lin K.M., Lin K.-H., Lin L.-T., Lin P.-H., Lin Q., Lin S., Lin S.-J., Lin W., Lin X., Lin Y.-X., Lin Y.-S., Linden R., Lindner P., Ling S.-C., Lingor P., Linnemann A.K., Liou Y.-C., Lipinski M.M., Lipovsek S., Lira V.A., Lisiak N., Liton P.B., Liu C., Liu C.-H., Liu C.-F., Liu C.H., Liu F., Liu H.-S., Liu H.-F., Liu H., Liu J., Liu L., Liu M., Liu Q., Liu W., Liu X.-H., Liu X., Liu Y., Livingston J.A., Lizard G., Lizcano J.M., Ljubojevic-Holzer S., LLeonart 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Viret C., Viscomi M.T., Visnjic D., Vitale I., Vocadlo D.J., Voitsekhovskaja O.V., Volonte C., Volta M., Vomero M., Von Haefen C., Vooijs M.A., Voos W., Vucicevic L., Wade-Martins R., Waguri S., Waite K.A., and Wakatsuki S.
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.Copyright © 2020 Informa UK Limited, trading as Taylor & Francis Group.
- Published
- 2021
30. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
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Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., Anton Z., Anwar T., Apetoh L., Apostolova N., Araki T., Araki Y., 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[Ovid has truncated this field to avoid Excel character limitations], Klionsky D.J., Abdel-Aziz A.K., Abdelfatah S., Abdellatif M., Abdoli A., Abel S., Abeliovich H., Abildgaard M.H., Abudu Y.P., Acevedo-Arozena A., Adamopoulos I.E., Adeli K., Adolph T.E., Adornetto A., Aflaki E., Agam G., Agarwal A., Aggarwal B.B., Agnello M., Agostinis P., Agrewala J.N., Agrotis A., Aguilar P.V., Ahmad S.T., Ahmed Z.M., Ahumada-Castro U., Aits S., Aizawa S., Akkoc Y., Akoumianaki T., Akpinar H.A., Al-Abd A.M., Al-Akra L., Al-Gharaibeh A., Alaoui-Jamali M.A., Alberti S., Alcocer-Gomez E., Alessandri C., Ali M., Alim Al-Bari M.A., Aliwaini S., Alizadeh J., Almacellas E., Almasan A., Alonso A., Alonso G.D., Altan-Bonnet N., Altieri D.C., Alvarez E.M.C., Alves S., Alves da Costa C., Alzaharna M.M., Amadio M., Amantini C., Amaral C., Ambrosio S., Amer A.O., Ammanathan V., An Z., Andersen S.U., Andrabi S.A., Andrade-Silva M., Andres A.M., Angelini S., Ann D., Anozie U.C., Ansari M.Y., Antas P., Antebi A., 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- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.Copyright © 2020 Informa UK Limited, trading as Taylor & Francis Group.
- Published
- 2021
31. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
- Author
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Werner, Brand-Saberi, Beate, Dong, X Charlie, Kenchappa, Chandra Shekar, Li, Zuguo, Lin, Yong, Oshima, Shigeru, Rong, Yueguang, Sluimer, Judith C, Stallings, Christina L, and Tong, Chun-Kit
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
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- 2021
32. The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants
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Vazquez-Vilar, M., García-Carpintero, Víctor, Selma, S, Bernabé-Orts, J.M, Sanchez-Vicente, J, Salazar-Sarasua, B, Ressa, A, de Paola, C, Ajenjo, M, Quintela, J.C, Fernández-del-Carmen, Asun, Granell, Antonio, Orzáez, D, Vazquez-Vilar, M., García-Carpintero, Víctor, Selma, S, Bernabé-Orts, J.M, Sanchez-Vicente, J, Salazar-Sarasua, B, Ressa, A, de Paola, C, Ajenjo, M, Quintela, J.C, Fernández-del-Carmen, Asun, Granell, Antonio, and Orzáez, D
- Abstract
CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like gene regulation. However, the design and assembly of multiplex constructs comprising tandemly arrayed guide RNAs (gRNAs) requires scarless cloning and is still troublesome due to the presence of repetitive sequences, thus hampering a more widespread use. Here we present a comprehensive extension of the software-assisted cloning platform GoldenBraid (GB), in which, on top of its multigene cloning software, we integrate new tools for the Type IIS-based easy and rapid assembly of up to six tandemly-arrayed gRNAs with both Cas9 and Cas12a, using the gRNA-tRNA-spaced and the crRNA unspaced approaches, respectively. As stress tests for the new tools, we assembled and used for Agrobacterium-mediated stable transformation a 17 Cas9-gRNAs construct targeting a subset of the Squamosa-Promoter Binding Protein-Like (SPL) gene family in Nicotiana tabacum. The 14 selected genes are targets of miR156, thus potentially playing an important role in juvenile-to-adult and vegetative-to-reproductive phase transitions. With the 17 gRNAs construct we generated a collection of Cas9-free SPL edited T plants harboring up to 9 biallelic mutations and showing leaf juvenility and more branching. The functionality of GB-assembled dCas9 and dCas12a-based CRISPR/Cas activators and repressors using single and multiplexing gRNAs was validated using a Luciferase reporter with the Solanum lycopersicum Mtb promoter or the Agrobacterium tumefaciens nopaline synthase promoter in transient expression in Nicotiana benthamiana. With the incorporation of the new web-based tools and the accompanying collection of DNA parts, the GB4.0 genome edition turns an all-in-one open platform for plant ge
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- 2021
33. Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector
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Uranga, M, Aragonés, V, Selma, S, Vázquez-Vilar, M, Orzáez, D, Daròs Arnau, José Antonio, Uranga, M, Aragonés, V, Selma, S, Vázquez-Vilar, M, Orzáez, D, and Daròs Arnau, José Antonio
- Abstract
Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas strategies in plants rely on genetic transformation using Agrobacterium tumefaciens to supply the gene editing reagents, such as Cas nucleases or the synthetic guide RNA (sgRNA). While Cas nucleases are constant elements in editing approaches, sgRNAs are target-specific and a screening process is usually required to identify those most effective. Plant virus-derived vectors are an alternative for the fast and efficient delivery of sgRNAs into adult plants, due to the virus capacity for genome amplification and systemic movement, a strategy known as virus-induced genome editing. We engineered Potato virus X (PVX) to build a vector that easily expresses multiple sgRNAs in adult solanaceous plants. Using the PVX-based vector, Nicotiana benthamiana genes were efficiently targeted, producing nearly 80% indels in a transformed line that constitutively expresses Streptococcus pyogenes Cas9. Interestingly, results showed that the PVX vector allows expression of arrays of unspaced sgRNAs, achieving highly efficient multiplex editing in a few days in adult plant tissues. Moreover, virus-free edited progeny can be obtained from plants regenerated from infected tissues or infected plant seeds, which exhibit a high rate of heritable biallelic mutations. In conclusion, this new PVX vector allows easy, fast and efficient expression of sgRNA arrays for multiplex CRISPR-Cas genome editing and will be a useful tool for functional gene analysis and precision breeding across diverse plant species, particularly in Solanaceae crops.
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- 2021
34. Consejos útiles para la realización de una TC-coronariografía
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Fernández, G.C., Velasco, M., Delgado, C., Vázquez, M., Vilar, M., and Tardáguila, F.
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- 2008
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35. La herencia del expresionismo. Sobre la discusion en Das Wort, Moscu, 1937/38
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Vilar, M. Loreto
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- 2011
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36. Siguan, Marisa: Schreiben an den Grenzen der Sprache. Studien zu Amery, Kerstesz, Semprun, Schalamow, Herta Muller und Aub
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Vilar, M. Loreto
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- 2015
37. Chapter 5 - Neurotrophins and Neurotrophin Receptors
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Franco, M.L., Comaposada-Baró, R., and Vilar, M.
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- 2020
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38. Dry-off and dairy cow udder health and welfare : Effects of different milk cessation methods
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Vilar, M. J., Rajala-Schultz, P. J., Departments of Faculty of Veterinary Medicine, Production Animal Medicine, Helsinki One Health (HOH), Ruminant health, Animal Reproduction Science, and University Management
- Subjects
Milk yield ,SOMATIC-CELL COUNT ,DIFFERENT FEEDING STRATEGIES ,Dry-off ,PERIOD ,food and beverages ,BOVINE MAMMARY-GLAND ,413 Veterinary science ,Milking frequency ,LACTATING COWS ,Cow welfare ,ABRUPT CESSATION ,SHORT-COMMUNICATION ,fluids and secretions ,PROLACTIN-RELEASE INHIBITION ,Udder health ,INTRAMAMMARY INFECTIONS ,Mammary involution ,INVOLUTION - Abstract
The cessation of milking at the end of lactation is a routine management practice in dairy herds, and the importance of the dry period for milk production and udder health, has long been recognized. Among countries and herds, drying-off practices differ and include various milk cessation methods, such as changes in milking frequency and in feeding, the use of antibiotic dry cow therapy and teat sealants, and changes in housing. Published studies reporting methods of stopping milk production are scarce, and there are no uniform recommendations on optimal procedures to dry cows off for good udder health, cow welfare, and milk production. This review describes methods to stop mik production to prepare cows for the dry period and their effects on mammary involution, udder health, and dairy cow welfare. Milk yield at dry-off (the final milking at the end of lactation) is important for rapid involution, which stimulates the immune system and promotes good udder health and cow welfare. Based on the findings of this review, gradual cessation of milking over several days before the final milking can effectively reduce milk yield at dry-off and accelerate mammary gland involution while maximizing cow comfort and welfare. Data from this review indicate a target production level of 15 kg/day of milk or less at dry-off. (c) 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Published
- 2020
39. Reversible colorimetric probes for mercury sensing
- Author
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Coronado, Eugenio, Galan-Mascaros, Jose R., Marti-Gastaldo, Carlos, Nazeeruddin, Md. K., Palomares, Emilio, Gratzel, Vilar M., Durrant, James R., and Vilar, Ramon
- Subjects
Colorimetric analysis -- Analysis ,Ruthenium -- Chemical properties ,Mercury compounds -- Chemical properties ,Chemistry - Abstract
The selectivity and sensitivity of two colorimetric sensors based on the ruthenium complexes bis(2,2'-bipyridyl-4,4'-dicarboxylate)ruthenium(II) bis (tetrabutylammonium) bis(thiocyanate) and (2,2':6',2''-terpyridine-4,4',4''-tricarboxylate)ruthenium(II) tris(tetrabutylammonium) tris(isothiocyanate) are described. Results reveal that mercury ions coordinate reversibly to the sulfur atom of the dyes' NCS groups.
- Published
- 2005
40. Culicoides vectors of bluetongue virus in Chester Zoo
- Author
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Vilar, M. J., Guis, H., Krzywinski, J., Sanderson, S., and Baylis, M.
- Published
- 2011
- Full Text
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41. Reengineering Ttr Amyloid Inhibition Properties of Diflunisal
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Saraiva, M, primary, Vilaró, M, additional, Nieto, J, additional, Almeida, R, additional, Ballesteros, A, additional, Arsequell, G, additional, Valencia, G, additional, Barluenga, J, additional, and Planas, A, additional
- Published
- 2007
- Full Text
- View/download PDF
42. EFFECTIVENESS AND TOLERABILITY OF PROLONGED LINEZOLID THERAPY FOR CHRONIC OSTEOMYELITIS
- Author
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Herruzo, A., Rodriguez, D., Pigrau, C., Vilar, M., Almirante, B., Pahissa, A., and Sanchez, Flores X.
- Published
- 2009
43. Bascoy, Montserrat / Silos Ribas, Lorena (eds.): Autobiographische Diskurse von Frauen (1900-1950)
- Author
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Vilar, M. Loreto
- Published
- 2018
- Full Text
- View/download PDF
44. Cotton functionalized with nanostructured TiO2-Ag-AgBr layer for solar photocatalytic degradation of dyes and toxic organophosphates
- Author
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Boufi, S., primary, Abid, M., additional, Bouattour, S., additional, Ferraria, A.M., additional, Conceição, D.S., additional, Ferreira, L.F. Vieira, additional, Corbel, G., additional, Neto, P.M., additional, Lopes, P.A., additional, Vilar, M. Rei, additional, and do Rego, A.M. Botelho, additional
- Published
- 2019
- Full Text
- View/download PDF
45. Strong gene activation with genome-wide specificity using a new orthogonal CRISPR/Cas9-based Programmable Transcriptional Activator
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Selma, S, primary, Bernabé-Orts, J, additional, Vazquez-Vilar, M, additional, Diego, B, additional, Ajenjo, M, additional, García-Carpintero, V, additional, Granell, A, additional, and Orzaez, D, additional
- Published
- 2018
- Full Text
- View/download PDF
46. SAMs functionalised by α-quaterthiophene as model for polythiophene grafting
- Author
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Nogues, C, Lang, P, Rei Vilar, M, Desbat, B, Buffeteau, T, El-Kassmi, A, and Garnier, F
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- 2002
- Full Text
- View/download PDF
47. HREELS characterisation of polymer surfaces using new generation spectrometers
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Rei Vilar, M. and Jugnet, Y.
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- 2002
- Full Text
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48. High-Resolution Electron Energy Loss Spectroscopy
- Author
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Pireaux, Jean-Jacques, primary, Grégoire, Ch., additional, Vermeersch, M., additional, Thiry, P. A., additional, Vilar, M. Rei, additional, and Caudano, R., additional
- Published
- 1990
- Full Text
- View/download PDF
49. Bascoy, Montserrat / Silos Ribas, Lorena (eds.): 'Autobiographische Diskurse von Frauen (1900-1950)'. Würzburg: Königshausen u. Neumann 2017. 256 pp.
- Author
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Vilar, M. Loreto and Vilar, M. Loreto
- Published
- 2018
50. Aspergillus niger N402 derivatives grown with different supplements
- Author
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Odoni, D.I., Vazquez Vilar, M., van Gaal, Merlijn, Schonewille, T., Martins dos Santos, V.A.P., Tamayo-Ramos, Juan Antonio, Suarez Diez, M., Schaap, P.J., Odoni, D.I., Vazquez Vilar, M., van Gaal, Merlijn, Schonewille, T., Martins dos Santos, V.A.P., Tamayo-Ramos, Juan Antonio, Suarez Diez, M., and Schaap, P.J.
- Abstract
Aspergillus niger N402 derivatives, harbouring an argB deletion and thus making them arginine auxothrophs, were grown with either 1.1 mM arginine or 5 mM citrulline added to the medium., Aspergillus niger N402 derivatives, harbouring an argB deletion and thus making them arginine auxothrophs, were grown with either 1.1 mM arginine or 5 mM citrulline added to the medium.
- Published
- 2018
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