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236 results on '"Vijayan, K. K."'

26. Reproductive performance of captive-reared Indian white shrimp, Penaeus indicus, broodstocks over two generations

Author

Anand, P. S. Shyne, primary, Balasubramanian, C. P., additional, Aravind, R., additional, Biju, I. F., additional, Rajan, R. Vidhu, additional, Vinay, T. N., additional, Panigrahi, A., additional, Sudheer, N. S., additional, Rajamanickam, S., additional, Kumar, Sujeet, additional, Antony, Jose, additional, Ambasankar, K., additional, Jithendran, K. P., additional, and Vijayan, K. K., additional

Published
2023
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27. Database on nutritional composition of food fishes from India

Author

Mohanty, Bimal Prasanna, Karunakaran, D., Mahanty, Arabinda, Ganguly, Satabdi, Debnath, Dipesh, Mitra, Tandrima, Banerjee, Sudeshna, Sharma, Anil Prakash, Sankar, T. V., Anandan, R., Mathew, Suseela, Asha, K. K., Chakraborty, Kajal, Vijayagopal, P., Paul, B. N., Sridhar, N., Chanda, S., Sarma, Debajit, Pandey, Nityanand, Shahi, Neetu, Das, Pushpita, Das, Partha, Akhtar, Md Shahbaz, Dayal, J. Syama, Vijayan, K. K., Kannappan, S., Venkateshwarlu, G., Singh, S. D., Mohan, Madan, Meenakumari, B., and Ayyappan, S.

Published
2015

29. Effect of Bacillus spp. on the composition of gut microbiota in early life stages of Indian white shrimp, Penaeus indicus.

Author

Patil, P. K., Vinay, T. N., Aravind, R., Avunje, S., and Vijayan, K. K.

Subjects
WHITELEG shrimp, GUT microbiome, BACILLUS (Bacteria), PROTEOBACTERIA, BACILLUS subtilis, MICROBIAL communities
Abstract

To study the effects of the probiotics on the gut microbial community of Penaeus indicus larvae, a combination of three probiotics—Bacillus subtilis, B. lichiniformis, and B. pumilus (5 × 1010 cfu/gram)—were added to the rearing environment of shrimp larvae (5,000/500 L). Altered microbial communities due to the application of probiotics were studied using 16S rRNA-based Illumina sequencing. The results suggest that the probiotics-treated group showed higher richness; the control group was even as measured by alpha diversity indices. The abundance of Proteobacteria was higher in the probiotic-treated group (94.85%) compared to the control (58.29%). At the genus level, the probiotics group was dominated by Vibrio (44.17%), Rhodobacteraceae_unclassified (25.22%), Catenococcus (12.89%), Pseudoalteromonas (4.76%), and Kordia (1.53%); Tenacibaculum (24.29%), Catenococcus (19.38%), Vibrio (7%), Gammaproteobacteria_unclassified (6.6%), Fluviicola (5.91%), and Chlamydiales_unclassified (4.61%) dominated in the control group. Results indicate that probiotic application influenced the taxonomic profile of gut microbes. This information helps us to understand the effect of probiotic application especially in penaeid shrimp hatcheries. [ABSTRACT FROM AUTHOR]

Published
2023
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32. Early Post-Vaccination Gene Signatures Correlate With the Magnitude and Function of Vaccine-Induced HIV Envelope-Specific Plasma Antibodies in Infant Rhesus Macaques

Author

Vijayan, K. K. Vidya, primary, Cross, Kaitlyn A., additional, Curtis, Alan D., additional, Van Rompay, Koen K. A., additional, Pollara, Justin, additional, Fox, Christopher B., additional, Tomai, Mark, additional, Hanke, Tomáš, additional, Fouda, Genevieve, additional, Hudgens, Michael G., additional, Permar, Sallie R., additional, and De Paris, Kristina, additional

Published
2022
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39. Effect of dietary soy lecithin inclusion levels on growth, feed utilization, fatty acid profile, deformity and survival of milkfish (Chanos chanos) larvae

Author

Sivaramakrishnan, T., primary, Ambasankar, K., additional, Kumaraguru Vasagam, K. P., additional, Syama Dayal, Jagabattula, additional, Sandeep, Kizhakkekarammal Puthiyedathu, additional, Bera, Aritra, additional, Makesh, M., additional, Kailasam, M., additional, and Vijayan, K. K., additional

Published
2021
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44. Visual loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Enterocytozoon hepatopenaei (EHP) infection

Author

Sathish Kumar T, Jithendran K. P, Vijayan K. K, Navaneeth Krishnan A, Joseph Sahaya Rajan J, Shankar Vinayakarao Alavandi, and M. Makesh

Subjects
0301 basic medicine, Loop-mediated isothermal amplification, Biology, Microsporidiosis, Polymerase Chain Reaction, Sensitivity and Specificity, law.invention, Feces, 03 medical and health sciences, Penaeidae, law, Enterocytozoon hepatopenaei, Spore wall, medicine, Animals, Parasite hosting, Polymerase chain reaction, DNA Primers, Detection limit, General Veterinary, fungi, General Medicine, Enterocytozoon, Ribosomal RNA, medicine.disease, Molecular biology, 030104 developmental biology, Infectious Diseases, Insect Science, Parasitology, Nucleic Acid Amplification Techniques
Abstract

The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP), the causative agent of hepatopancreatic microsporidiosis, has been widely reported in shrimp-farming countries. EHP infection can be detected by light microscopy observation of spores (1.7 × 1 μm) in stained hepatopancreas (HP) tissue smears, HP tissue sections, and fecal samples. EHP can also be detected by polymerase chain reaction (PCR) targeting the small subunit (SSU) ribosomal RNA (rRNA) gene or the spore wall protein gene (SWP). In this study, a rapid, sensitive, specific, and closed tube visual loop-mediated isothermal amplification (LAMP) protocol combined with FTA cards was developed for the diagnosis of EHP. LAMP primers were designed based on the SSU rRNA gene of EHP. The target sequence of EHP was amplified at constant temperature of 65 °C for 45 min and amplified LAMP products were visually detected in a closed tube system by using SYBR™ green I dye. Detection limit of this LAMP protocol was ten copies. Field and clinical applicability of this assay was evaluated using 162 field samples including 106 HP tissue samples and 56 fecal samples collected from shrimp farms. Out of 162 samples, EHP could be detected in 62 samples (47 HP samples and 15 fecal samples). When compared with SWP-PCR as the gold standard, this EHP LAMP assay had 95.31% sensitivity, 98.98% specificity, and a kappa value of 0.948. This simple, closed tube, clinically evaluated visual LAMP assay has great potential for diagnosing EHP at the farm level, particularly under low-resource circumstances.

Published
2018

49. Phylogenetic diversity and predictive functional profile of bacteria associated with marine microalgae, Isochrysis galbana using next generation sequencing.

Author

Sandhya, S. V. and Vijayan, K. K.

Subjects
*PHYLOGENY, *MICROALGAE, *DNA sequencing, *SUSTAINABILITY, *PROTEOBACTERIA
Abstract

In aquaculture, microalgal-bacterial interaction has ecological significance, and thus demands better understanding for improvement of sustainability and productivity of large scale microalgal cultivation. Here, we assessed the bacterial diversity, including the uncultivable bacterial assemblage associated with the marine microalgae Isochrysis galbana using next generation sequencing approach. Isochrysis has been considered as one of the most favoured types of live feed in aquaculture and hence, chose Isochrysis galbana MBTDCMFRI S002. Total genomic DNA was extracted from I. galbana culture and 16S rDNA V3 region was sequenced with an Illumina MiSeq platform. A total of 30 different known bacterial genera were detected from 1190 identified operational taxonomic units. These bacterial phylotypes were affiliated to Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Actinobacteria, Acidimicrobiia, Bacteroidia, Flavobacteriia and Bacilli classes. These 30 bacterial genera comprise only 4.62% of the total OTUs obtained and remaining 95.38% of the sequences do not exhibit any similarity against known bacterial genera in the taxonomic database. The functional profile of bacterial communities was predicted using PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) analysis. The results indicated that these associated bacterial communities are mainly involved in environmental as well as genetic information processing, membrane transport and nutrient metabolism. These functions may mediate their interaction with the phytoplankton host, and thus improve bacterial survival in microalgal habitat. Overall, the present study enhances the understanding of microalgal-bacterial interaction in terms of diversity and functional role of associated microbial community. [ABSTRACT FROM AUTHOR]

Published
2022

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