209 results on '"Vignoles, Philippe"'
Search Results
2. Laboratory Cultures of Lymnaeidae for Parasitological Experiments
- Author
-
Dreyfuss, Gilles, Vignoles, Philippe, Rondelaud, Daniel, Sánchez, Jorge, Vázquez, Antonio A., Feldhaar, Heike, Series Editor, Schmidt-Rhaesa, Andreas, Series Editor, Vinarski, Maxim V., editor, and Vázquez, Antonio A., editor
- Published
- 2023
- Full Text
- View/download PDF
3. Laboratory Cultures of Lymnaeidae for Parasitological Experiments
- Author
-
Dreyfuss, Gilles, primary, Vignoles, Philippe, additional, Rondelaud, Daniel, additional, Sánchez, Jorge, additional, and Vázquez, Antonio A., additional
- Published
- 2023
- Full Text
- View/download PDF
4. Control of Fasciolosis-Transmitting Lymnaeids in the Field
- Author
-
Rondelaud, Daniel, primary, Vignoles, Philippe, additional, Dreyfuss, Gilles, additional, Pointier, Jean-Pierre, additional, and Vázquez, Antonio A., additional
- Published
- 2023
- Full Text
- View/download PDF
5. Proteome characterization in various biological fluids of Trypanosoma brucei gambiense-infected subjects
- Author
-
Bonnet, Julien, Garcia, Camille, Leger, Thibaut, Couquet, Marie-Pauline, Vignoles, Philippe, Vatunga, Gedeao, Ndung'u, Joseph, Boudot, Clotilde, Bisser, Sylvie, and Courtioux, Bertrand
- Published
- 2019
- Full Text
- View/download PDF
6. Diversity of Toxoplasma gondii strains shaped by commensal communities of small mammals
- Author
-
Galal, Lokman, Schares, Gereon, Stragier, Claire, Vignoles, Philippe, Brouat, Carine, Cuny, Thomas, Dubois, Camille, Rohart, Thao, Glodas, Clément, Dardé, Marie-Laure, Kane, Mamadou, Niang, Youssoupha, Diallo, Mamoudou, Sow, Aliou, Aubert, Dominique, Hamidović, Azra, Ajzenberg, Daniel, and Mercier, Aurélien
- Published
- 2019
- Full Text
- View/download PDF
7. Fasciola hepatica larval development within the intermediate host.
- Author
-
Dreyfuss, Gilles, primary, Sindou, Philippe, additional, Hourdin, Philippe, additional, Vignoles, Philippe, additional, and Rondelaud, Daniel, additional
- Published
- 2021
- Full Text
- View/download PDF
8. Local low dose curcumin treatment improves functional recovery and remyelination in a rat model of sciatic nerve crush through inhibition of oxidative stress
- Author
-
Caillaud, Martial, Chantemargue, Benjamin, Richard, Laurence, Vignaud, Laetitia, Favreau, Frédéric, Faye, Pierre-Antoine, Vignoles, Philippe, Sturtz, Franck, Trouillas, Patrick, Vallat, Jean-Michel, Desmoulière, Alexis, and Billet, Fabrice
- Published
- 2018
- Full Text
- View/download PDF
9. Interactions between Galba truncatula (Gastropoda, Lymnaeidae) and two land snail species on the acid soils of central France.
- Author
-
Rondelaud, Daniel, Vignoles, Philippe, and Dreyfuss, Gilles
- Abstract
Interactions between Galba truncatula and two species of land snails (Cochlicopa lubrica, Succinea putris) that colonise lymnaeid habitats in June-July were studied by introducing G. truncatula into new sites (Experiment A) or removing it from its natural habitats (Experiment B). Snail counts were performed in late May or early June for the eight years following introduction or removal of G. truncatula. The results were compared to those observed at control sites where G. truncatula was absent. In sites where G. truncatula was introduced (Experiment A), the mean density of the lymnaeid peaked in the second year and gradually decreased in subsequent years. Conversely, the mean density of the land snails decreased sharply in the second year before increasing rapidly for S. putris and gradually for C. lubrica in subsequent years. Removal of G. truncatula from its natural habitats (Experiment B) had no significant effect on the mean density of C. lubrica. In contrast, that of S. putris peaked in the second year and decreased rapidly to lower values in subsequent years. Density interactions between G. truncatula and the two land snail species indicate that there is probably competition between these species for space in lymnaeid habitats in May and June. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. Changes in the Populations of Two Lymnaeidae and Their Infection by Fasciola hepatica and/or Calicophoron daubneyi over the Past 30 Years in Central France
- Author
-
Rondelaud, Daniel, primary, Vignoles, Philippe, additional, and Dreyfuss, Gilles, additional
- Published
- 2022
- Full Text
- View/download PDF
11. Fasciola hepatica: the dispersal of cercariae shed by the snail Galba truncatula
- Author
-
Rondelaud Daniel, Vignoles Philippe, and Dreyfuss Gilles
- Subjects
fasciola hepatica ,limousin ,metacercaria ,nasturtium officinale ,watercress bed ,Infectious and parasitic diseases ,RC109-216 - Abstract
Field investigations in 14 wild watercress beds located in the French region of Limousin, a known endemic area for distomatosis, were performed for three years to determine the distance that cercariae of Fasciola hepatica can reach in water before their encystment on the host plant. Each bed was located on the course of an open drainage furrow, while snails (Galba truncatula) lived upstream around the emergence of a source. Five plant species were collected in early April and examined to find metacercariae. Most cysts were noted on Nasturtium officinale (188 on 48.7 kg of dripped plants), followed by Helosciadium nodiflorum (125 on 33.4 kg). On the other plant species, there were few larvae. Most cercariae encysted on the plants growing in the most upstream part of each bed, usually on the first 50 cm in length. When water in the beds was fast running, the distribution of metacercariae was more limited and their number was fewer than those in the beds fed by a slow flow of water. Cercariae were able to swim or were carried away by the current up to a mean of 5 m in slow-flow waters before encysting; this distance was only 4 m in faster waters. Plants growing on the most upstream section of a watercress bed located in a drainage furrow are the most used by cercariae for their encystment, when snails live around the emergence of a source. The speed of the water current affected the number and distribution of metacercariae in the bed.
- Published
- 2020
- Full Text
- View/download PDF
12. The detection of snail host habitats in liver fluke infected farms by use of plant indicators
- Author
-
Rondelaud, Daniel, Hourdin, Philippe, Vignoles, Philippe, Dreyfuss, Gilles, and Cabaret, Jacques
- Published
- 2011
- Full Text
- View/download PDF
13. Decline in the number and size of populations of two Lymnaeidae living in central France over the last decade
- Author
-
Vignoles, Philippe, primary, Dreyfuss, Gilles, additional, and Rondelaud, Daniel, additional
- Published
- 2022
- Full Text
- View/download PDF
14. Determination of zones at risk for fasciolosis in the department of Haute-Vienne, central France: a retrospective study on natural infections detected in 108,481 Galba truncatula for 37 years
- Author
-
Vignoles Philippe, Rondelaud Daniel, and Dreyfuss Gilles
- Subjects
altitude ,at risk zones ,climate ,fasciola hepatica ,galba truncatula ,haute-vienne ,snails ,Infectious and parasitic diseases ,RC109-216 - Abstract
A retrospective study on the natural infection of Galba truncatula by Fasciola hepatica was carried out in the French department of Haute-Vienne to determine whether there are areas at risk for fasciolosis. Adult snails included in this analysis came from samples collected from pastures on 259 farms and from 121 wild watercress beds between 1970 and 2006. Fasciola hepatica infection rates were examined in relation to altitude and climatic data (mean annual rainfall, mean annual temperature) of each municipality. In a total of 108,481 snails collected in 151 municipalities, the overall prevalence of infection was 3.8% but varied according to the municipalities from which samples were taken (from 1% to 7.4%). The prevalence of F. hepatica infection in snails significantly decreased when the mean altitude of municipalities or their mean annual rainfall increased. However, this prevalence significantly increased with increasing mean annual temperatures. Studying the prevalence of infection in these snails makes it possible to delineate zones at risk for fasciolosis on the acid soils of Haute-Vienne. The risk of infection for livestock would be greater in areas of Haute-Vienne below 400 m above sea level and would gradually decrease when the altitude of the land increases.
- Published
- 2017
- Full Text
- View/download PDF
15. Distribution of metacercariae of several Digenea in wild watercress beds crossed by running water
- Author
-
Vignoles, Philippe, Hourdin, Philippe, Rondelaud, Daniel, and Dreyfuss, Gilles
- Subjects
métacercaire ,Calicophoron daubneyi ,Nasturtium officinale ,Fasciola hepatica ,Biology ,biology.organism_classification ,food.food ,cressonnière ,metacercaria ,food ,Helosciadium nodiflorum ,Botany ,watercress bed - Abstract
Plusieurs espèces de Digènes utilisent le mollusque Galba truncatula comme hôte intermédiaire pour le développement de leurs formes larvaires. Les cercaires, qui sont émises par la limnée, s’enkystent sur diverses plantes aquatiques comme le cresson. Si la liste de ces plantes hôtes est assez bien connue à l’heure actuelle, aucune information n’est, par contre, disponible sur la dispersion et la distribution de ces cercaires par rapport à l’espèce de la plante hôte. Des investigations ont donc été réalisées pendant trois années dans 14 cressonnières naturelles situées dans des rigoles de drainage superficiel et traversées par de l’eau courante. Dans chaque rigole, la population de G. truncatula vivait autour de l'émergence d'une source, tandis que la cressonnière était située plus en aval sur le cours même de la rigole. Cinq espèces végétales ont été récoltées au début du mois d'avril et examinées au stéréomicroscope pour y trouver des métacercaires. Des kystes appartenant à quatre Digènes : Calicophoron daubneyi, Fasciola hepatica, Notocotylus sp. et un Echinostomatidé non identifié ont été observés sur ces plantes. La plupart d’entre eux ont été observés sur Nasturtium officinale (243 sur 48,7 kg de feuilles et de tiges égouttées), suivis par Helosciadium nodiflorum (164 sur 33,4 kg). Sur les autres espèces végétales, il y avait peu de larves : de 18 à 25 par espèce. La plupart des cercaires de F. hepatica, de Notocotylus sp. et de l’Echinostomatidé se sont enkystées sur les feuilles supérieures et la zone des tiges situées juste sous la surface de l’eau, tandis que celles de C. daubneyi se sont fixées sur les feuilles basales et le collet des plantes. De plus, les plantes, sur lesquelles la plupart des cercaires se sont enkystées, poussaient dans la partie la plus en amont de chaque cressonnière (habituellement sur les premiers 50 cm de longueur). Lorsque l'eau courante dans les cressonnières était plus rapide, la distribution des métacercaires était plus limitée et leur nombre était inférieur à celui observé dans les stations alimentées par un lent débit d'eau. Les plantes qui poussent sur la section la plus en amont d'une cressonnière située dans une rigole de drainage superficiel sont les plus utilisées par les cercaires des quatre Digènes pour leur enkystement. La vitesse du courant d'eau affecte le nombre et la distribution des métacercaires dans une cressonnière., Several species of Digenea use the snail Galba truncatula as an intermediate host for the development of their larval forms. Cercariae, which exited from the snail, encyst on various aquatic plants such as watercress. Although the list of these host plants is fairly well known at present, no information is available on the dispersion and distribution of these cercariae relative to the host plant species. Field investigations were therefore carried out for three years in 14 wild watercress beds located in open drainage furrows and crossed by running water. In each furrow, the population of G. truncatula lived around the emergence of a spring, while the bed was located further downstream on the course of the furrow. Five plant species were harvested in early April and examined for metacercariae using a stereomicroscope. Cysts belonging to four Digenea : Calicophoron daubneyi, Fasciola hepatica, Notocotylus sp. and an unidentified echinostomatid were observed on these plants. Most of these were observed on Nasturtium officinale (243 on 48.7 kg of drained leaves and stems), followed by Helosciadium nodiflorum (164 on 33.4 kg). On the other plant species, there were few larvae : from 18 to 25 per plant species. Most of the F. hepatica, Notocotylus sp. and echinostomatid cercariae formed cysts on the upper leaves and the area of stems just under the water surface, while those of C. daubneyi settled on the basal leaves and collar of the plants. In addition, the plants, on which most of these cercariae are encysted, grew in the most upstream part of each watercress bed (usually on the first 50 cm in length). When running water in the watercress bed was fast, the distribution of metacercariae was more limited and fewer in number than in the stations fed by a slow flow of water. Plants that grow on the most upstream section of a watercress bed in an open drainage furrow are the most used by cercariae of the four Digenea for their encystment. The speed of the water flow affects the number and distribution of metacercariae in the bed., Annales Scientifiques du Limousin, Tome 29 | 2020
- Published
- 2020
- Full Text
- View/download PDF
16. The impact of global warming on the intermediate hosts of Fasciola hepatica in Haute Vienne
- Author
-
Rondelaud, Daniel, Vignoles, Philippe, Hourdin, Philippe, and Dreyfuss, Gilles
- Subjects
Galba truncatula ,education.field_of_study ,biology ,Population ,population ,Zoology ,Fasciola hepatica ,biology.organism_classification ,Lymnaeidae ,Habitat ,Omphiscola glabra ,education - Abstract
Dans le département de la Haute-Vienne, deux espèces de limnées : Galba truncatula et Omphiscola glabra, sont connues pour être des hôtes intermédiaires dans le cycle de vie d’un parasite, Fasciola hepatica. Ces deux mollusques montrent, à l’heure actuelle, un déclin dans le nombre des populations et la superficie de leurs habitats. Pour expliquer ce déclin, le réchauffement climatique a été proposé comme l’un des facteurs possibles. Comme plusieurs épisodes de canicule ont eu lieu dans le département en 2015, 2018 et 2019, des investigations ont été effectuées en 2020 dans 190 prairies marécageuses pour dénombrer les populations de chaque espèce, les individus transhivernants de chaque population et préciser la superficie de leurs habitats. Si l’on ne tient pas compte du type d’habitat, le nombre global des populations en 2020 a diminué de 37,5 % chez G. truncatula et de 23,7 % chez O. glabra par rapport aux relevés effectués par notre équipe dans les mêmes prairies en 2013-2014. De même, la densité globale des individus transhivernants a présenté une diminution de 74,7 % chez G. truncatula et de 55,4 % chez O. glabra. L’ampleur de la chute pour ces deux paramètres a présenté des variations selon le type d’habitat. Par contre, les habitats de G. truncatula et la plupart des sites avec O. glabra n’ont pas montré de variation significative dans leur superficie entre les deux périodes d’étude. Entre 2013-2014 et 2020, 11 populations de G. truncatula et six d’O. glabra se sont déplacées vers l’aval sur le cours même des rigoles de drainage et parfois jusqu’au fossé principal. Sept habitats peuplés par les deux espèces de limnées ont également été observés dans les prairies étudiées en 2020 alors qu’il n’y en avait pas en 2013-2014. Ces changements dans l’écologie et la dynamique des deux espèces de limnées doivent être rapportés en grande partie à l’effet des épisodes de canicule qui ont eu lieu dans le département au cours des années passées., In the French department of Haute Vienne, two lymnaeid species, i.e. Galba truncatula and Omphiscola glabra, are known to be intermediate hosts in the development cycle of the parasite Fasciola hepatica. These two snails currently show a decline in the number of their populations and area of their habitats. To explain this decline, global warming was proposed as one of the possible factors. As several heat waves took place in the department in 2015, 2018 and 2019, investigations were carried out in 2020 in 190 swampy meadows to count the populations of each species, the overwintering individuals in each population and to specify the area of their habitats. Compared to values recorded by our team on the same grasslands in 2013-2014, the overall number of populations in 2020 decreased by 37.5 % for G. truncatula and by 23.7 % for O. glabra. Similarly, the overall density of overwintering snails showed a decrease of 74.7 % for G. truncatula and 55.4 % for O. glabra. The magnitude of the decline for these two parameters varied according to habitat type. In contrast, G. truncatula habitats and most sites with O. glabra did not show any significant variation in their area between the two study periods. Between 2013-2014 and 2020, 11 populations of G. truncatula and six of O. glabra have moved downstream on the course of the drainage furrows and sometimes to the main ditch. Seven habitats populated by the two lymnaeid species were also observed in the meadows studied in 2020 when there was none in 2013-2014. These changes in the ecology and dynamics of lymnaeid populations must be partly related to the effect of heat waves that have occurred in the department in the past years., Annales Scientifiques du Limousin, Tome 29 | 2020
- Published
- 2020
- Full Text
- View/download PDF
17. Current decline in the number and size of Galba truncatula and Omphiscola glabra populations, intermediate hosts of Fasciola hepatica, on the acidic soils of Central France
- Author
-
Dreyfuss Gilles, Vignoles Philippe, and Rondelaud Daniel
- Subjects
Galba truncatula ,Omphiscola glabra ,Population decline ,Snail density ,Snail habitat ,Infectious and parasitic diseases ,RC109-216 - Abstract
Field investigations on the habitats colonized by Galba truncatula or Omphiscola glabra were carried out on 162 farms of the Limousin region, Central France, to determine whether there is currently a decline in the number and size of snail populations. Seven types of snail habitats were considered here. Compared to the numbers of snail populations recorded from 1976 to 1992, the values noted from 2013 to 2016 were significantly lower, with a decline rate of 34% for G. truncatula and 23% for O. glabra. Variations in this decline rate with the type of snail habitat were also noted. The greatest decreases in the numbers of snail populations were noted for spring heads located in meadows and for road ditches, while the lowest were noted for open drainage furrows present in meadows. The distribution of these habitats according to their area did not show any significant change over time. In contrast, overwintering snails were significantly less numerous in 2013–2016 in five types of habitats for G. truncatula and in three types only for O. glabra. Several causes underlie this population decline. Among them, the current development of mechanical cleaning in open drainage systems and road ditches, that of subsurface drainage in meadows, and regular gyro-crushing of vegetation around temporary spring heads were the most important.
- Published
- 2016
- Full Text
- View/download PDF
18. The control of Galba truncatula (Gastropoda: Lymnaeidae) by the terrestrial snail Zonitoides nitidus on acid soils
- Author
-
Rondelaud, Daniel, Vignoles, Philippe, Dreyfuss, Gilles, and Mage, Christian
- Published
- 2006
- Full Text
- View/download PDF
19. Galba truncatula (O.F. Müller, 1774) (Gastropoda, Lymnaeidae): the colonization of new stations on acid soil by low numbers of snails
- Author
-
Dreyfuss, Gilles, primary, Vignoles, Philippe, additional, and Rondelaud, Daniel, additional
- Published
- 2021
- Full Text
- View/download PDF
20. Répartition des métacercaires de quatre Digènes dans des cressonnières naturelles traversées par de l’eau courante
- Author
-
Vignoles, Philippe, primary, Hourdin, Philippe, additional, Rondelaud, Daniel, additional, and Dreyfuss, Gilles, additional
- Published
- 2020
- Full Text
- View/download PDF
21. Les conséquences du réchauffement climatique sur les hôtes intermédiaires de Fasciola hepatica en Haute-Vienne
- Author
-
Rondelaud, Daniel, primary, Vignoles, Philippe, additional, Hourdin, Philippe, additional, and Dreyfuss, Gilles, additional
- Published
- 2020
- Full Text
- View/download PDF
22. Larval trematode infections in Lymnaea glabra populations living in the Brenne Regional Natural Park, central France
- Author
-
Rondelaud Daniel, Vignoles Philippe, and Dreyfuss Gilles
- Subjects
Brenne Regional Natural Park ,Cercaria ,Digenean ,Lymnaea glabra ,Lymnaeidae ,Snail ,Infectious and parasitic diseases ,RC109-216 - Abstract
Lymnaea glabra is known to be a natural intermediate host of two flukes, Calicophoron daubneyi and Fasciola hepatica, in central France. But it can also sustain larval development of other digeneans. Adult snails were thus collected from 206 habitats in 2014 and 2015 to identify parasite species and determine the prevalence of each digenean infection in relation to the five types of snail habitats. Seven digenean species were noted in 321 infected snails (out of 17,647 L. glabra). Snails with F. hepatica or C. daubneyi were found in 14.5% and 12.6% of habitats, respectively. Percentages were lower for snails with Opisthoglyphe ranae (5.8%), Haplometra cylindracea (5.3%) and were less than 5% for those infected with Echinostoma revolutum, Notocotylus sp. or Plagiorchis sp. Prevalence noted for each parasite species varied with the type of habitat. The number of species in L. glabra was lower than that found in G. truncatula from the same region (7 instead of 10). The distribution and prevalence of each digenean species were thus dependent on the type and location of each snail habitat.
- Published
- 2015
- Full Text
- View/download PDF
23. Interferon gamma effect on immune mediator production in human nerve cells infected by two strains of Toxoplasma gondii
- Author
-
Mammari Nour, Vignoles Philippe, Halabi Mohamad Adnan, Dardé Marie-Laure, and Courtioux Bertrand
- Subjects
Toxoplasma gondii ,Human nerve cells ,IFN-γ ,Immunity ,Infectious and parasitic diseases ,RC109-216 - Abstract
Interferon gamma (IFN-γ) is the major immune mediator that prevents toxoplasmic encephalitis in murine models. The lack of IFN-γ secretion causes reactivation of latent T. gondii infection that may confer a risk for severe toxoplasmic encephalitis. We analyse the effect of IFN-γ on immune mediator production and parasite multiplication in human nerve cells infected by tachyzoites of two T. gondii strains (RH and PRU). IFN-γ decreased the synthesis of MCP-1, G-CSF, GM-CSF and Serpin E1 in all cell types. It decreased IL-6, migration inhibitory factor (MIF) and GROα synthesis only in endothelial cells, while it increased sICAM and Serpin E1 synthesis only in neurons. The PRU strain burden increased in all nerve cells and in contrast, RH strain replication was controlled in IFN-γ-stimulated microglial and endothelial cells but not in IFN-γ-stimulated neurons. The proliferation of the PRU strain in all stimulated cells could be a specific effect of this strain on the host cell.
- Published
- 2015
- Full Text
- View/download PDF
24. Pseudosuccinea columella: age resistance to Calicophoron daubneyi infection in two snail populations
- Author
-
Dar Yasser, Rondelaud Daniel, Vignoles Philippe, and Dreyfuss Gilles
- Subjects
Calicophoron daubneyi ,Cercaria ,Experimental infections ,Prevalence ,Pseudosuccinea columella ,Rediae ,Infectious and parasitic diseases ,RC109-216 - Abstract
Individual infections of Egyptian and French Pseudosuccinea columella with five miracidia of Calicophoron daubneyi were carried out to determine whether this lymnaeid was capable of sustaining larval development of this parasite. On day 42 post-exposure (at 23 °C), infected snails were only noted in groups of individuals measuring 1 or 2 mm in height at miracidial exposure. Snail survival in the 2-mm groups was significantly higher than that noted in the 1-mm snails, whatever the geographic origin of snail population. In contrast, prevalence of C. daubneyi infection was significantly greater in the 1-mm groups (15–20% versus 3.4–4.0% in the 2-mm snails). Low values were noted for the mean shell growth of infected snails at their death (3.1–4.0 mm) and the mean number of cercariae (
- Published
- 2015
- Full Text
- View/download PDF
25. Fasciola hepatica: comparative metacercarial productions in experimentally-infected Galba truncatula and Pseudosuccinea columella
- Author
-
Vignoles Philippe, Dreyfuss Gilles, and Rondelaud Daniel
- Subjects
Experimental infections ,Fasciola hepatica ,Galba truncatula ,Metacercaria ,Pseudosuccinea columella ,Infectious and parasitic diseases ,RC109-216 - Abstract
As large numbers of metacercariae of Fasciola hepatica are necessary for research, experimental infections of Galba truncatula and Pseudosuccinea columella with this digenean were carried out to determine the better intermediate host for metacercarial production and, consequently, the most profitable snail for decreasing the cost price of these larvae. Pre-adult snails (4 mm in shell height) originating from two populations per lymnaeid species were individually exposed to two or five miracidia, raised at 23 °C and followed for cercarial shedding up to their death. Compared to values noted in G. truncatula, the survival of P. columella on day 30 post-exposure was significantly greater, while the prevalence of F. hepatica infection was significantly lower. In the four P. columella groups, metacercarial production was significantly greater than that noted in the four groups of G. truncatula (347–453 per cercariae-shedding snail versus 163–275, respectively). Apart from one population of G. truncatula, the use of five miracidia per snail at exposure significantly increased the prevalence of F. hepatica in P. columella and the other population of G. truncatula, whereas it did not have any clear effect on the mean number of metacercariae. The use of P. columella for experimental infections with F. hepatica resulted in significantly higher metacercarial production than that noted with G. truncatula, in spite of a lower prevalence for the former lymnaeid. This finding allows for a significant decrease in the cost price of these larvae for commercial production.
- Published
- 2015
- Full Text
- View/download PDF
26. Detection of Toxoplasma gondii DNA in horse meat from supermarkets in France and performance evaluation of two serological tests
- Author
-
Aroussi Abdelkrim, Vignoles Philippe, Dalmay François, Wimel Laurence, Dardé Marie-Laure, Mercier Aurélien, and Ajzenberg Daniel
- Subjects
Toxoplasma gondii ,Horse meat ,Enzyme-linked immunosorbent assay ,Modified agglutination test ,Magnetic-capture polymerase chain reaction ,Infectious and parasitic diseases ,RC109-216 - Abstract
In France, some cases of severe toxoplasmosis have been linked to the consumption of horse meat that had been imported from the American continent where atypical strains of Toxoplasma gondii are more common than in Europe. Many seroprevalence studies are presented in the literature but risk assessment of T. gondii infection after horse meat consumption is not possible in the absence of validated serological tests and the unknown correlation between detection of antibodies against T. gondii and presence of tissue cysts. We performed magnetic-capture polymerase chain reaction (MC-PCR) to detect T. gondii DNA in 231 horse meat samples purchased in supermarkets in France and evaluated the performance and level of agreement of the modified agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA) in the meat juices. The serological tests lacked sensitivity, specificity, and agreement between them, and there was no correlation with the presence of T. gondii DNA in horse meat, raising concerns about the reliability of T. gondii seroprevalence data in horses from the literature. T. gondii DNA was detected in 43% of horse meat samples but the absence of strain isolation in mice following inoculation of more than 100 horse meat samples suggests a low distribution of cysts in skeletal muscles and a low risk of T. gondii infection associated with horse meat consumption. However, to avoid any risk of toxoplasmosis, thorough cooking of horse meat is recommended.
- Published
- 2015
- Full Text
- View/download PDF
27. Galba truncatula (O.F. Müller, 1774) and Omphiscola glabra (O.F. Müller, 1774) (Mollusca: Lymnaeidae): influence of altitude on the characteristics of populations living on the acidic soils of Haute Vienne
- Author
-
Dreyfuss, Gilles, Vignoles, Philippe, and Rondelaud, Daniel
- Subjects
Galba truncatula ,biology ,population ,biology.organism_classification ,écologie ,Lymnaeidae ,Omphiscola glabra ,Botany ,sol acide ,ecology ,acidic soil - Abstract
Une étude rétrospective sur les populations de Galba truncatula et d’Omphiscola glabra vivant sur sols acides a été réalisée afin de déterminer l’influence de l’altitude sur leur distribution et les caractéristiques de leurs habitats. La fréquence des populations dans chaque municipalité et le nombre des individus transhivernants dans chaque population ont présenté une diminution significative lorsque l’altitude moyenne augmente. Un résultat similaire a été noté pour la superficie des habitats occupés par G. truncatula. Par contre, celle des sites colonisés par O. glabra n’a pas montré de variation significative par rapport à l’altitude. Ces résultats peuvent s’expliquer par la diminution de la température avec l’altitude et/ou par une plus grande acidité des sols lorsqu’ils sont au-dessus de 500 m d’altitude en Haute-Vienne., A retrospective study on the populations of Galba truncatula and Omphiscola glabra living on acidic soils was carried out to determine the influence of altitude on their distribution and the characteristics of their habitats. The frequency of populations in each municipality and the number of overwintering individuals in each population decreased significantly with increasing mean altitude. A similar result was noted for the area of habitats occupied by G. truncatula. On the other hand, that of sites colonized by O. glabra did not show any significant variation in relation to altitude. These results can be explained by the drop in temperature with the increase in altitude and/or by a greater acidity of the soils above 500 m in altitude in Haute Vienne., Annales Scientifiques du Limousin, Tome 28 | 2019
- Published
- 2019
- Full Text
- View/download PDF
28. Can Omphiscola glabra adapt to new acid soil stations when low numbers of snails are introduced?
- Author
-
Rondelaud, Daniel, Vignoles, Philippe, and Dreyfuss, Gilles
- Subjects
biology ,colonisation ,habitat ,Forestry ,colonization ,biology.organism_classification ,Lymnaeidae ,Transplantation ,Colonisation ,Omphiscola glabra ,acid soil ,sol acide ,transplantation - Abstract
Des échantillons de 5, 10 ou 20 Omphiscola glabra adultes ont été introduits dans 12 fossés de route ou de chemin sur sol acide pour étudier la colonisation de ces nouveaux sites par le mollusque. Des relevés ont été ensuite effectués pendant les six années suivantes pour déterminer la superficie que ces individus colonisent dans ces nouvelles stations et décompter le nombre des individus transhivernants en avril ou en mai. De nouvelles populations se sont développées dans les 12 fossés étudiés. La colonisation de chaque collection d’eau a été plus rapide lorsque l’échantillon de mollusques introduits comprenait 20 adultes. L’effectif global des descendants décomptés en avril ou en mai est passé par un pic au cours de la deuxième année post-introduction avant de diminuer graduellement jusqu’à une moyenne de 32,3-33,5 mollusques à la sixième année. La Limnée étroite est donc capable de coloniser de nouvelles zones sur sol acide à condition qu’il y ait une source temporaire., Samples of 5, 10 or 20 adult Omphiscola glabra were introduced into 12 road or way ditches on acid soil to study the colonization of these new sites by the snail. Surveys were conducted over the following six years to determine the area these O. glabra colonized in these new stations and to count the number of overwintering snails in April or May. New populations developed in the 12 ditches. Colonization of each water collection was faster when the sample of introduced snails included 20 adults. The overall number of offspring counted in April or May peaked in the second year post-introduction before gradually decreasing to a mean of 32.3-33.5 snails in the sixth year. Omphiscola glabra is therefore able to colonize new areas on acid soil so long as there is a temporary source., Annales Scientifiques du Limousin, Tome 28 | 2019
- Published
- 2019
- Full Text
- View/download PDF
29. Galba truncatula (O.F. Müller, 1774): ecological observations and biometric data on two morphotypes of snails living on acid soils
- Author
-
Vignoles, Philippe, Rondelaud, Daniel, and Dreyfuss, Gilles
- Subjects
Galba truncatula ,dynamique des populations ,biology ,Botany ,population dynamics ,biometry ,biométrie ,biology.organism_classification ,morphotype ,Lymnaeidae - Abstract
Des observations écologiques et des études biométriques ont été effectuées pendant trois années sur deux populations de Galba truncatula avec des coquilles présentant des tours convexes peu étagés (Châteauponsac, Chavanat) et les deux autres avec des coquilles présentant des tours en marches d’escalier (témoins). La densité des limnées tout au long de l’année était significativement plus élevée dans les populations de Châteauponsac et de Chavanat : une moyenne de 194.m-2 et de 180.m-2, respectivement, en mai ou juin, en fonction de l’altitude au lieu de 41.m-2 et de 34.m-2 dans les habitats témoins. Des résultats similaires ont également été notés pour la densité des pontes au printemps et le nombre d’oeufs par ponte. La croissance des mollusques tout au long de l’année n’a été étudiée que dans deux populations et n’a pas présenté de différence significative entre les quatre populations. Les limnées de Châteauponsac et de Chavanat ont une coquille plus étroite que celles des populations témoins, aussi bien pour la largeur du dernier tour de spire que pour celle de l’ouverture. Devant ces résultats, les auteurs proposent de classer provisoirement les deux populations de Châteauponsac et de Chavanat comme une variété de G. truncatula en attendant qu’un malacologue expérimenté en systématique précise le rang taxonomique de ces deux populations., Ecological observations and biometric studies were carried out over three years on two populations of Galba truncatula with shells showing convex whorls without steps (Châteauponsac, Chavanat) and the other two populations with shells having characteristic step-like whorls (controls). Snail density throughout the year was significantly higher in the Châteauponsac and Chavanat populations: an average of 194.m-2 and 180.m-2, respectively, in May or June, depending on altitude instead of 41.m-2 and 34.m-2 in control habitats. Similar results were also noted for egg-mass density in spring and the number of eggs per mass. Snail growth throughout the year was only studied in two populations and showed no significant difference between the four populations. The snails of Châteauponsac and Chavanat had a narrower shell than those of the control populations, as well for the width of the last whorl as for that of the aperture. Given these results, the authors propose to temporarily classify temporarily the two populations of Châteauponsac and Chavanat as a variety of G. truncatula while waiting for studies by a malacologist experienced in systematics to specify the taxonomic rank of these two populations., Annales Scientifiques du Limousin, Tome 28 | 2019
- Published
- 2019
- Full Text
- View/download PDF
30. Epidemiology of fasciolosis in Limousin: review of research carried out since the 1970s
- Author
-
Vignoles, Philippe, HOURDIN, Philippe, Dreyfuss, Gilles, and Rondelaud, Daniel
- Subjects
biology ,parasitology ,Forestry ,medicine.disease ,biology.organism_classification ,parasitologie ,Lymnaeidae ,Geography ,fasciolose ,medicine ,fasciolosis ,Fasciolosis ,control ,contrôle - Abstract
Les auteurs dressent un bilan des recherches qu’ils ont effectuées depuis 1970 sur plusieurs espèces de Lymnaeidae dans le Limousin afin de déterminer leur rôle comme hôtes intermédiaires dans la transmission de la fasciolose humaine, de préciser plusieurs points dans le développement larvaire du parasite chez ces mollusques et de mettre au place un contrôle contre la limnée la plus fréquente, Galba truncatula. La fasciolose a fortement reculé dans la région depuis les années 2000 en raison de l’abandon du cresson « sauvage » dans la consommation humaine. Plusieurs espèces de limnées ont été identifiées dans ces cressonnières et trois modes d’infestation par Fasciola hepatica ont été mis en évidence. Chez G. truncatula, plusieurs générations rédiennes se succèdent dans le temps. La production de cercaires présente une variabilité en fonction des populations de limnées et également à l’intérieur d’une même population. Lors de l’évolution des formes larvaires du parasite, la limnée présente une pathologie au niveau de ses viscères. Dans les prairies sur sol acide, trois espèces d'escargots peuvent y vivre avec des caractéristiques propres pour chaque limnée. Deux types de contrôle pour G. truncatula ont été étudiés, l’un biologique basé sur des escargots prédateurs (Zonitoides nitidus) au début de l’assèchement estival, et l’autre intégré prenant en compte les différentes mesures connues pour déparasiter l’hôte définitif et éliminer la limnée. Les résultats obtenus lors de l’étude de ces différents thèmes sont analysés et plusieurs hypothèses de travail sont proposées pour les chercheurs à venir., The authors take stock of the research they have carried out since 1970 on several species of Lymnaeidae in Limousin to determine their role as intermediate hosts in the transmission of human fasciolosis, to specify several points in the larval development of the parasite in these species. and to develop a control against the most common lymnaeid, Galba truncatula. Fasciolosis has declined sharply in the region since the 2000s due to the abandonment of "wild" watercress in human consumption. Several species of lymnaeids were identified in these watercress beds and three modes of snail infection with Fasciola hepatica were highlighted. In G. truncatula, several generations of rediae succeeded one another in time. In this species, the production of cercariae varied according to the lymnaeid population and also within the same population. During the development of parasite larval forms, the snail showed tissue lesions in its viscera. In the meadows on acid soil, three species of snails can live there with specific characteristics for each lymnaeid. Two types of control for G. truncatula were studied, one being biological with the use of predatory snails (Zonitoides nitidus) at the beginning of the summer drying, and the other integrated taking into account the various known measures to deworm the definitive host and eliminate the lymnaeid.. The results obtained during the study of these different themes are analyzed and several working hypotheses are proposed for future researchers., Annales Scientifiques du Limousin, Tome 28 | 2019
- Published
- 2019
- Full Text
- View/download PDF
31. Ruminal paramphistomosis in cattle from northeastern Algeria: prevalence, parasite burdens and species identification
- Author
-
Titi Amal, Mekroud Abdeslam, Chibat Mohamed el Hadi, Boucheikhchoukh Mehdi, Zein-Eddine Rima, Djuikwo-Teukeng Félicité F., Vignoles Philippe, Rondelaud Daniel, and Dreyfuss Gilles
- Subjects
Algeria ,Calicophoron daubneyi ,C. microbothrium ,Cattle ,Intensity of infection ,Molecular identification ,Paramphistomosis ,Prevalence ,Infectious and parasitic diseases ,RC109-216 - Abstract
Slaughterhouse samples were analysed over a two-year period (September 2010–August 2012) in Jijel (northeastern Algeria) in order to determine seasonal variations in the prevalence and intensity of bovine paramphistomosis in a Mediterranean climate and identify paramphistome species using molecular biology. In spring and summer, significantly higher prevalences and lower parasite burdens were noted in bull calves, thus indicating an effect of season on these parameters. In contrast, the differences among seasonal prevalences or among seasonal parasite burdens were not significant in the case of old cows. Eleven adult worms from the slaughterhouses of Jijel and three neighbouring departments (Constantine, El Tarf and Setif) were analysed using molecular markers for species identification. Two different species, Calicophoron daubneyi and C. microbothrium, were found. The presence of these two paramphistomids raises the question of their respective frequency in the definitive host and local intermediate hosts.
- Published
- 2014
- Full Text
- View/download PDF
32. Radix natalensis: the effect of Fasciola hepatica infection on the reproductive activity of the snail
- Author
-
Dar Yasser, Vignoles Philippe, Rondelaud Daniel, and Dreyfuss Gilles
- Subjects
Egg ,Egg-laying ,Fasciola hepatica ,Radix natalensis ,Snail infection ,Infectious and parasitic diseases ,RC109-216 - Abstract
Experimental infections of Egyptian Radix natalensis (shell height at miracidial exposure: 4 mm) with a French isolate of Fasciola hepatica were carried out under laboratory conditions at 22 °C to specify the characteristics and follow the dynamics of their egg-laying. Controls constituted unexposed R. natalensis of the same size. No significant difference between controls and the uninfected snails of the exposed group was noted, whatever the parameter considered. In controls and exposed snails, the dates of the first egg masses were close to each other (56.4–65.3 days). In contrast, the life span of snails and the length of the egg-laying period were significantly shorter and egg production was significantly lower in infected R. natalensis than in controls and uninfected snails. In infected R. natalensis, but without cercarial shedding (NCS snails), egg production was irregular throughout the egg-laying period. In cercarial-shedding (CS) snails, the first egg masses were laid before the first cercarial emergence (at a mean of 56 days and 67 days, respectively). Thereafter, egg mass production of CS snails was irregular up to day 72 of the experiment, stopped during the following two weeks and started again after day 88 for a single snail. In conclusion, the F. hepatica infection of R. natalensis reduced the reproductive activity in both NCS and CS snails. The pattern noted for egg production in infected R. natalensis seems to be species-specific because of the high shell size of this lymnaeid and its role as an atypical intermediate host in the life cycle of the parasite.
- Published
- 2014
- Full Text
- View/download PDF
33. Fasciola hepatica: effect of the natural light level on cercarial emergence from temperature-challenged Galba truncatula
- Author
-
Vignoles Philippe, Titi Amal, Rondelaud Daniel, Mekroud Abdeslam, and Dreyfuss Gilles
- Subjects
Cercaria ,Cercarial emergence ,Light ,Fasciola hepatica ,Galba truncatula ,Temperature ,Infectious and parasitic diseases ,RC109-216 - Abstract
As abrupt changes in water temperature (thermal shock) triggered a significantly greater cercarial emergence of Fasciola hepatica from experimentally infected Galba truncatula, laboratory investigations were carried out to study the influence of light on cercarial emergence in snails subjected to a thermal shock every week (a mean of 12 °C for 3 h) during the patent period. Thermal shock for these temperature-challenged (TC) snails was carried out outdoors under artificial or natural light, or indoors under constant artificial light. Compared with the infected control snails always reared indoors at 20 °C, the number of cercariae in TC snails subjected to a thermal shock and natural light outdoors was significantly greater. The repetition of this experiment by subjecting TC snails to the same thermal shock indoors under an artificial light level ranging from 600 to 3000 lux did not show any significant difference among the numbers of cercariae in the different subgroups. A detailed analysis of the results noted in the TC snails subjected to natural light during the thermal shock demonstrated that the number of cercariae-releasing snails was significantly higher between 601 and 1200 lux and for the highest nebulosity values (7–8 octas, which corresponds to a sufficiently or completely overcast sky). Contrary to the intensity of artificial light, which did not influence cercarial emergence, the natural light level had a significant effect on this process when F. hepatica-infected snails were subjected to a regular thermal shock during the patent period.
- Published
- 2014
- Full Text
- View/download PDF
34. Consequence of temperature changes on cercarial shedding from Galba truncatula infected with Fasciola hepatica or Paramphistomum daubneyi
- Author
-
Rondelaud Daniel, Titi Amal, Vignoles Philippe, Mekroud Abdeslam, and Dreyfuss Gilles
- Subjects
Cercaria ,Cercarial shedding ,Fasciola hepatica ,Galba truncatula ,Paramphistomum daubneyi ,Temperature change ,Infectious and parasitic diseases ,RC109-216 - Abstract
Experimental infections of Galba truncatula (two populations) with Fasciola hepatica or Paramphistomum daubneyi were carried out to study the effect of water temperature changes (3 h at a mean of 12 °C every week) on cercarial shedding during the patent period. The results were compared with those of control snails infected according to the same protocol and always maintained at 20 °C. Compared to controls, a significant increase in the number of cercariae-shedding snails, a significantly longer patent period and significantly greater cercarial production were noted in temperature-challenged snails, regardless of the type of digenean infection. In contrast, the number of incompletely formed metacercariae was significantly higher in temperature-challenged snails than in controls. Incompletely formed metacercariae of F. hepatica consisted of cysts whose colour remained whitish after shedding (25.4% for temperature-challenged snails) or whose dome was flattened after encystment (74.6%). Those of P. daubneyi were totally dark brown or blackish after formation. These incomplete metacercariae might originate from young differentiating cercariae within the snail body (F. hepatica) or from cercariae which died just after encystment (P. daubneyi). The use of regular temperature changes for snails infected with F. hepatica or P. daubneyi must be monitored carefully during collection of metacercariae to select completely formed cysts for infecting definitive hosts.
- Published
- 2013
- Full Text
- View/download PDF
35. May the habitats of the snail Galba truncatula (Gastropoda, Lymnaeidae) be found using plant indicators on the acid soils of Limousin ?
- Author
-
Rondelaud, Daniel, Hourdin, Philippe, VIGNOLES, Philippe, and Dreyfuss, Gilles
- Subjects
Galba truncatula ,mollusque ,snail ,habitat ,acid soil ,plante indicatrice ,sols acides ,indicator plant - Abstract
Des investigations de terrain ont été réalisées pendant 30 années en mars ou avril dans 361 fermes élevant des bovins ou des ovins afin de recenser les plantes indicatrices pour chaque type d’habitat colonisé par le mollusque Galba truncatula et établir des corrélations avec deux paramètres caractérisant ces sites (superficie du gîte, densité des mollusques). Sept types d’habitat et six espèces de plantes indicatrices ont été reconnus dans les 7.709 sites étudiés. Les habitats les plus fréquents se situent à l’extrémité périphérique des rigoles de drainage superficiel (84,1 % des sites sont colonisés) et autour des sources (81,4 %). Les gîtes dont la superficie inférieure à 3 m2 sont principalement localisés à l’extrémité des rigoles de drainage, le long des fossés correspondants et autour des sources lorsque Juncus acutiflorus, Juncus effusus et Lotus uliginosus sont les plantes indicatrices. Dans les fossés de route et le long des berges de rivière ou d’étang, des superficies plus élevées ont été notées dans la plupart des cas. Les plus fortes densités en mollusques (> 25/m2) ont été observées dans le cas de J. acutiflorus (52,5 % des habitats) et de L. uliginosus (56,7 %). Des densités plus faibles (< 25 mollusques/m2) ont été notées dans le cas de J. effusus (88,8 % des habitats) alors que celles trouvées dans le cas de Glyceria fluitans, d’Agrostis stolonifera et de Dactylis glomerata sont souvent inférieures à 10 mollusques/m2. Le Jonc acutiflore est une bonne espèce indicatrice pour les habitats de G. trun-catula situés dans les prairies et les fossés de route qui les bordent. Même s’il est moins représentatif dans les autres types de gîtes, sa large distribution en Europe permet de l’utiliser pour identifier la plupart des habitats de la limnée sur l’ensemble des sols acides présents dans les pays européens., Field investigations in 361 cattle- or sheep-breeding farms on acid soil were carried out during thirty years in March-April to record indicator plants in relation to the category of habitat colonized by the snail Galba truncatula and to establish correlations with two parameters characterizing these sites (habitat area, snail density). Seven types of snail habitats and six species of indicator plants were listed in the 7,709 sites studied. The most frequent habitats were located at the peripheral extremities of open drainage furrows (84.1 % of places colonized) and around spring heads (81.4 %). Lower than 3-m2 sites were essentially found at the extremities of drainage furrows, along drainage ditches or around spring heads when Juncus acutiflorus, Juncus effusus, or Lotus uliginosus are indicator plants. In road ditches and along pond or river banks, greater values were found in most cases. The highest snail densities (>25 snails/m2) were observed in the case of J. acutiflorus (52.5 % of habitats) and L. uliginosus (56.7 %). Lower snail densities (, Annales Scientifiques du Limousin, Tome 20 | 2009
- Published
- 2018
- Full Text
- View/download PDF
36. Galba truncatula (Lymnaeidae): the colonisation of the habitat over the year and the dynamics of annual generations in two populations living along river banks upstream from a dam
- Author
-
Rondelaud, Daniel, Hourdin, Philippe, ABROUS, M., VIGNOLES, Philippe, and Dreyfuss, Gilles
- Subjects
Galba truncatula ,biology ,Ecology ,berges de rivière ,biology.organism_classification ,acid soils ,écologie ,Geography ,ethology ,éthologie ,river banks ,ecology ,sols acides - Abstract
Des investigations malacologiques ont été réalisées pendant trois années sur deux habitats de Galba truncatula, situés sur des berges de rivière en amont d'un barrage afin d’étudier la colonisation des différentes zones de l’habitat par les limnées en fonction du niveau de l’eau, de déterminer la dynamique des générations annuelles du mollusque et d'analyser les capacités de la limnée à s'exonder. Les deux habitats présentent un retrait quotidien de l'eau, ce qui permet l'étude des berges pendant plusieurs heures. De mars à décembre, les limnées des deux populations suivent les variations saisonnières du niveau de l'eau mais peu d'entre elles suivent le retrait quotidien de celle-ci. Deux générations annuelles de G. truncatula ont été notées dans ces habitats, la première à partir du début de juillet et la seconde en décembre. De juillet à octobre, les limnées en activité effectuent d'assez grands déplacements (25 à 44 cm) lorsque l'eau se retire chaque jour mais elles présentent aussi une réaction de fuite vers les zones émergées lorsque celle-ci remonte. Durant la même période, la croissance des mollusques qui suivent chaque jour le retrait de l'eau est significativement plus rapide que celle des mollusques qui ne se déplacent que dans la même zone de leur habitat. Ces résultats suggèrent que les populations de G. truncatula vivant sur le bord de ces rivières seraient parfaitement adaptées à leur habitat., Malacological investigations in two habitats of Galba truncatula, located along river banks upstream from a dam, were carried out over three years to study the colonization of the different zones of the habitat by snails in relation to seasonal water-level variations, to determine the dynamics of snail's annual generations, and to analyse the ability of the snail to emerge from water. These snail habitats showed a daily retirement of water, thus permitting the study of snails on banks for several hours per day. From March to December, snails followed the seasonal variations of water-level. However, only a few of them were observed following the daily retirement of water. Two annual generations of G. truncatula, the first from the onset of July and the second in December, were noted in these habitats. From July to October, non aestivating snails travelled a mean of 25 to 44 cm when water retired every day. However, they escaped water rising by taking refuge in the still emerged zones of the habitats. When snails followed the daily retirement of water, their growth from July to October was significantly quicker than that of snails which only moved in the same zone of their habitat. The populations of G. truncatula living along river banks would be completely adapted to their habitat., Annales Scientifiques du Limousin, Tome 16 | 2005
- Published
- 2018
- Full Text
- View/download PDF
37. Annual generations of Galba truncatula (Mollusca, Lymnaeidae) in the depart-ment of Haute-Vienne
- Author
-
Rondelaud, Daniel, VIGNOLES, Philippe, Hourdin, Philippe, and Dreyfuss, Gilles
- Subjects
Galba truncatula ,générations annuelles ,annual generations ,sols acides ,acid soils - Abstract
Des investigations ont été réalisées pendant trois années dans cinq habitats à Limnées tronquées situés dans le département de la Haute-Vienne afin de connaître les périodes de pontes et suivre la croissance des individus en fonction de leur génération annuelle. Dans les trois fossés où circule de l’eau tout au long de l’année, une seule généra-tion annuelle de G. truncatula a été notée avec dépôt des pontes de la fin juin jusqu’au début d’octobre. Dans les deux autres habitats soumis au dessèchement estival (août et septembre), deux générations ont été observées et les pontes ont eu lieu de la mi-avril à la fin juin et de novembre à décembre. Même s’il existe des variations d’effectif et de croissance dans le cas des habitats permanents, les risques parasitaires liés aux populations présentant une seule génération annuelle sont très limités en raison du faible nombre de ces colonies dans le nord de la Haute-Vienne (3 seulement sur un total de 1.731 détectés dans les prairies de ce secteur)., Field investigations were carried out over a 3-year period in five habitats of Galba truncatula located in the department of Haute-Vienne to determine periods of egg-laying and to follow the growth of these snails in relation to their annual generation. In the three ditches with running water throughout the year, a single annual generation of G. truncatula was found and egg-masses were laid from the end of June up to the beginning of October. In the other two habitats subjected to summer drying (the sites were dried in August and September), two generations were noted and egg-deposits occurred from mid-April to the end of June and in November-December. Even if variations in the numbers of snails and their growth were found in these three permanent habitats, the parasitic risks caused by these populations showing a single annual generation were limited owing to the low number of these communities in northern Haute-Vienne (three only out of a total of 1,731 detected in these meadows of this zone)., Annales Scientifiques du Limousin, Tome 19 | 2008
- Published
- 2018
- Full Text
- View/download PDF
38. Ecological and parasitological changes caused by the introduction of a freshwater gastropod, Omphiscola glabra (Lymnaeidae) into new sites
- Author
-
VIGNOLES, Philippe, Dreyfuss, Gilles, and Rondelaud, Daniel
- Subjects
Lymnaei-dae ,Galba truncatula ,parasitology ,Omphiscola glabra ,prairies ,acid soil ,sols acides ,parasitologie ,écologie ,Lymnaeidae ,meadows - Abstract
Des échantillons d’Omphiscola glabra provenant d’une région calcaire (La Brenne, département de l’Indre) ont été introduits dans de nouveaux sites en Haute-Vienne pour étudier l’évolution de ces mollusques étrangers sur des sols acides et suivre leur infestation naturelle par Fasciola hepatica ou Paramphistomum daubneyi. L’opération inverse a été effectuée avec des limnées de la Haute-Vienne transférées dans la Brenne tandis que des mollusques de chaque région, implantés localement ont servi de témoins. Chez les O. glabra vivant en Brenne comme chez les limnées locales implantées en Haute-Vienne, le nombre d’adultes par population et la superficie de leurs habitats passent par des pics au cours de la troisième année ou de la quatrième année post-introduction (p.i.). Chez les limnées de la Brenne introduites en Haute-Vienne, on assiste à une diminution progressive des valeurs pour les deux paramètres précités à partir de la deuxième année p.i. Des mollusques parasités naturellement ont été retrouvés dans toutes les populations d’O. glabra. Chez les limnées de la Brenne introduites en Haute-Vienne comme chez celles qui vivent en Brenne, la prévalence et l’intensité de leur infestation naturelle par l’un ou l’autre des Digènes s’accroissent progressivement à partir de la deuxième année p.i. ou de la troisième. Par contre, les valeurs de ces paramètres chez les limnées de la Haute-Vienne implantées localement ne présentent pas de variation significative au cours des années. En 2009, la prévalence de l’infestation par F. hepatica est significativement plus élevée chez les O. glabra de la Haute-Vienne implantées localement ou dans la Brenne que chez une autre limnée (Galba truncatula) vivant dans les mêmes prairies. Les variations observées pour les effectifs d’O. glabra et la superficie de ses habitats peuvent s’expliquer par la concentration en calcium présente dans le sol de chaque région. L’accroissement de la prévalence ou de l’intensité de leur infestation naturelle peut s’interpréter comme une adaptation progressive de cette limnée à une pression parasitaire différente lorsque cette espèce est introduite dans un nouveau milieu., Samples of Omphiscola glabra, originating from a calcareous region (La Brenne, department of Indre), were introduced into new sites located in Haute-Vienne to study the development of these foreigners on acid soil and to follow their natural infection with Fasciola hepatica and/or Paramphistomum daubneyi. An inverse process using lymnaeids collected from Haute-Vienne and placed in Brenne was also performed, while snails originating from each region and locally introduced were used as controls. In all samples of O. glabra living in Brenne and the local snails in Haute-Vienne, the number of adults per population and the area of their habitats peaked during the third or fourth year post-introduction (p.i.), respectively. In the foreign samples placed in Haute-Vienne, the values of these both parameters progressively decreased from the second year p.i. Naturally-infected snails were found in all populations of O. glabra. In all samples living in Brenne and the foreign snails in Haute-Vienne, the prevalence and intensity of snail infections by either of both Digenea progressively increased from the third or the second year p.i., respectively. In contrast, the values of these parameters in the local snails living in Haute-Vienne did not show any significant variation throughout the years. In 2009, the preva-lence of F. hepatica-infected O. glabra was significantly greater in the local samples living in Haute-Vienne and the foreigners in Brenne than in another lymnaeid Galba truncatula, living in the same meadows. The numerical variations noted for the numbers of O. glabra and their habitat areas can be explained by calcium concentrations present in the soils from each region. The increase in the prevalence and intensity of their natural infection may be interpreted as an adaptation of this snail to another parasitic pressure when this species is introduced into a new site., Annales Scientifiques du Limousin, Tome 21 | 2010
- Published
- 2018
- Full Text
- View/download PDF
39. The integrated control of Galba truncatula (Mollusca, Lymnaeidae) in natural watercress beds on acid soils
- Author
-
Rondelaud, Daniel, VIGNOLES, Philippe, Dreyfuss, Gilles, and Hourdin, Philippe
- Subjects
molluscicide ,Galba truncatula ,Biology ,cressonnières ,contrôle intégré ,biology.organism_classification ,predatory snails ,mollusques prédateurs ,acid soils ,Molluscicide ,integrated control ,Botany ,watercress beds ,sols acides - Abstract
Les Limnées tronquées vivant dans 134 cressonnières naturelles sur sols acides ont été soumises depuis les années 1990 à un contrôle intégré associant l’emploi d’un molluscicide (CuCl2) à faibles doses (0,1 mg/L) en mars-avril et l’introduction de mollusques prédateurs (Zonitoides nitidus) en juin-juillet. Ce traitement a été appliqué en prenant des précautions sur le plan environnemental et sur celui de la santé publique. Une seule année de contrôle a permis d’éliminer Galba truncatula des 134 cressonnières et Omphiscola glabra de neuf points d’eau (sur les 11 que cette limnée colonise). Deux années successives de contrôle ont été nécessaires pour faire disparaître O. glabra des deux autres cressonnières. De faibles concentrations de cuivre ont été trouvées dans la végétation de cinq stations au cours de la première année post-traitement. Le repeuplement de quelques sites par les limnées a été constaté à partir de la deuxième année après le contrôle. Mais aucune recontamination des cressonnières traitées par les métacercaires de Fasciola hepatica n’a été trouvée jusqu’à ce jour. Cette technique simple est à proposer aux propriétaires de cressonnières naturelles lorsqu’ils refusent de détruire leurs plantations, malgré la détection de cas de fasciolose parmi leurs proches., An integrated control of Galba truncatula by the use of a molluscicide (CuCl2) at low doses (0.1 mg/L) in March-April and the introduction of carnivorous snails (Zonitoides nitidus) in June-July was applied since the 1990s in 134 natural watercress beds on acid soils. Several precautions to avoid environmental risks and eventual consequences on public health were also applied. At the end of the first year of control, the elimination of Galba truncatula from the 134 watercress beds and that of Omphiscola glabra from 9 water holes (of the 11 colonized by this lymnaeid) were noted. Two years of control were necessary to eradicate O. glabra from the other two beds. Low concentrations of copper in watercress originating from 5 beds were noted during the first year after control. Several beds were recolonized by lymnaeids from the second year post-treatment. However, an epidemiological surveillance of these beds performed during years following snail control did not show any recontamination of watercress by metacercariae of Fasciola hepatica. This simple method must be proposed to the owners of natural watercress beds when they refuse to destroy beds in spite of the detection of one or several cases of human fasciolosis among their family and their relations., Annales Scientifiques du Limousin, Tome 16 | 2005
- Published
- 2018
- Full Text
- View/download PDF
40. Neopterin and CXCL-13 in Diagnosis and Follow-Up of Trypanosoma brucei gambiense Sleeping Sickness: Lessons from the Field in Angola
- Author
-
Bonnet, Julien, primary, Vignoles, Philippe, additional, Tiberti, Natalia, additional, Gedeão, Vatunga, additional, Hainard, Alexandre, additional, Turck, Natacha, additional, Josenando, Theophile, additional, Ndung’u, Joseph M, additional, Sanchez, Jean-Charles, additional, Courtioux, Bertrand, additional, and Bisser, Sylvie, additional
- Published
- 2019
- Full Text
- View/download PDF
41. La Limnée étroite est-elle capable de s’adapter à de nouvelles stations sur sol acide lorsque de faibles effectifs sont utilisés ?
- Author
-
Rondelaud, Daniel, primary, Vignoles, Philippe, additional, and Dreyfuss, Gilles, additional
- Published
- 2019
- Full Text
- View/download PDF
42. Galba truncatula (O.F. Müller, 1774) et Omphiscola glabra (O.F. Müller, 1774) (Mollusques : Lymnaeidae) : influence de l’altitude sur les caractéristiques des populations vivant sur les sols acides de la Haute-Vienne
- Author
-
Dreyfuss, Gilles, primary, Vignoles, Philippe, additional, and Rondelaud, Daniel, additional
- Published
- 2019
- Full Text
- View/download PDF
43. Epidémiologie de la fasciolose dans le Limousin : bilan des recherches effectuées depuis les années 1970
- Author
-
Vignoles, Philippe, primary, Hourdin, Philippe, additional, Dreyfuss, Gilles, additional, and Rondelaud, Daniel, additional
- Published
- 2019
- Full Text
- View/download PDF
44. Galba truncatula (O.F. Müller, 1774) : observations écologiques et données biométriques sur deux morphotypes de limnées vivant sur des sols acides
- Author
-
Vignoles, Philippe, primary, Rondelaud, Daniel, additional, and Dreyfuss, Gilles, additional
- Published
- 2019
- Full Text
- View/download PDF
45. Summary-cartography of the distribution of lymnaeidea snails and related species in the Basse-Marche (northern district of the Haute-Vienne department, Central France)
- Author
-
Rondelaud, Daniel, VIGNOLES, Philippe, Dreyfuss, Gilles, XUEREB, Fabien, ASPOSTOLOFF, Cyril, and VAREILLE-MOREL, Christiane
- Subjects
cartographie ,cartography ,Lymnaeidae - Abstract
Des investigations ont été réalisées dans 316 fossés de route, étangs et rivières de la Basse-Marche pour recenser les espèces de Pulmonés aquatiques et effectuer une cartographie de leur distribution selon un carroyage de 5 x 5 km. Dix espèces et 484 populations ont été recensées dans ce secteur. Quatre espèces prédominent par leur distribution et le nombre de leurs habitats. Lymnaea truncatula se rencontre sur toutes les mailles de la Basse-Marche et colonise 80 % des fossés étudiés, 71 % des étangs et 30 % des rivières mais son abondance est assez faible dans 70 % des sites. La répartition de Physa acuta est assez identique à celle de L. truncatula mais cette espèce se rencontre surtout dans les étangs et les rivières (76 % et 60,2 % respectivement). Lymnaea glabra et L. ovata ont une distribution plus limitée, avec une concentration des populations dans les vallées parcourues par des rivières importantes. La fréquence de la première espèce est de 23 % dans les fossés et de 12 % dans les étangs ; par contre, L. ovata colonise 36 % des étangs prospectés et 74 % des stations situées sur les ruisseaux et les rivières. Les six autres espèces de Pulmonés (quatre limnées, une physe et une planorbe) ont une distribution en taches, avec un nombre d'habitats réduit et une concentration des gîtes (pour trois d'entre elles) dans les carrés nord de l'arrondissement (Azat-le-Ris et communes environnantes)., Field investigations were carried out in 316 road ditches, ponds and rivers of the BasseMarche to list the freshwater pulmonate species and to draw maps of their distribution using 5-km squares. Ten species and 484 populations were found in the district studied. Four pulmonate species were way ahead by their distribution and the number of their habitats. Lymnaea truncatula was found in all the squares of the Basse-Marche and colonized 80 % of road ditches, 71 % of ponds, and 30 % of rivers. However, the number of overwintering snails in each population was low in 70 % of habitats. A similar finding was noted for the distribution of Physa acuta but this snail was especially found in ponds and rivers (76.0 % and 60.2 %, respectively). The distribution of Lymnaea glabra and L. ovata was more limited and their populations were concentrated in the valleys of large rivers. The frequency of L. glabra habitats was 23 % in road ditches and 12 % in ponds ; in contrast, L. ovata inhabited 36 % of the ponds studied and 74 % of stations on brooks and rivers. The other six pulmonate species (four lymnaeid, one physidid, and one planorbid species) had a spotted distribution and a low number of habitats. Three of these species were only found in the commune of Azat-le-Ris and other neighbouring squares., Annales Scientifiques du Limousin, Tome 11 | 2001
- Published
- 2018
- Full Text
- View/download PDF
46. Les larves de diptères Sciomyzidae dans le département de la Haute-Vienne. Leur impact sur le système Galbra truncatula - Fasciola hepatica
- Author
-
Dreyfuss, Gilles, VIGNOLES, Philippe, and Rondelaud, Daniel
- Abstract
Annales Scientifiques du Limousin, Tome 13 | 2002
- Published
- 2018
- Full Text
- View/download PDF
47. Premières données écologiques et biométrique sur quelques bytinelles (Gastéropodes, Hydrobiidae) vivant dans les ruisseaux de la région Limousin
- Author
-
VIGNOLES, Philippe, Rondelaud, Daniel, Dreyfuss, Gilles, and VAREILLE-MORE, Christiane
- Abstract
Annales Scientifiques du Limousin, Tome 13 | 2002
- Published
- 2018
- Full Text
- View/download PDF
48. Indicator plants and detection of habitats colonized by the snail Omphiscola glabra on the acid soils of Limousin
- Author
-
Rondelaud, Daniel, Hourdin, Philippe, VIGNOLES, Philippe, and Dreyfuss, Gilles
- Subjects
Omphiscola glabra ,habitat ,acid soil ,sol acide ,plante indicatrice ,indicator plant - Abstract
Des investigations de terrain ont été effectuées pendant 12 mois dans quatre habitats d'Omphiscola glabra situés sur marne ou sur granite afin de préciser le nombre et la taille des pontes au printemps et de suivre la croissance des deux générations annuelles au cours de l'année. Dans les deux types d'habitats, le nombre des pontes par mollusque adulte et le nombre des oeufs par ponte diminuent de manière significative au cours des sept semaines de la période d'oviposition (printemps 2015). Au cours de ces semaines, chaque limnée adulte sur marne dépose 6,4 pontes et 93,3 oeufs en moyenne. Sur granite, les valeurs moyennes sont respectivement de 5,7 pontes et de 69,1 oeufs. Deux générations annuelles, l'une au printemps et l'autre en automne, ont été notées dans ces habitats. Les hauteurs des individus nés sur marne sont significativement plus élevées que celles des mollusques nés sur granite. Il en résulte une taille maximale différente pour les adultes : 19,8 et 20,5 mm en moyenne sur marne au lieu de 17,2 et 18,2 mm sur granite. Deux catégories d'individus, à savoir des juvéniles nés au printemps (3,1 à 3,4 mm en moyenne en juillet) et des limnées adultes nées lors de l'année précédente (au printemps et en automne), subissent le dessèchement estival de leurs habitats. La différence existant entre les hauteurs de coquille sur marne et sur granite pourrait s'expliquer en partie par la teneur en ions calcium dissous dans l'eau courante (26-35 mg/L sur marne au lieu de teneurs inférieures à 20 mg/L sur granite)., Field investigations in four Omphiscola glabra populations living on marl or granite were carried out during 12 months to specify the number and size of egg-deposits in spring, and follow the shell growth of both generations over the year. In both types of habitats, the number of egg-masses per adult snail and their size significantly decreased during the seven weeks of snail egg-laying (spring 2015). During this period, each adult snail on marl laid a mean of 6.4 egg-masses and 93.3 eggs. On granite, the respective mean values were 5.7 egg-deposits and 69.1 eggs. Two annual generations, the first in spring and the other in autumn, were noted in these habitats. Shell heights of snails hatched on marl were significantly higher than those of snails living on granite. Consequently, the maximal shell height of adults was different : a mean of 19.8 and 20.5 mm on marl instead of 17.2 and 18.2 mm on granite. Two snail categories, i.e. spring-born juveniles (a mean of 3.1-3.4 mm in July) and adults born during the previous year (in spring and autumn), were subjected to summer drying of their habitats. The difference between the shell heights recorded on marl and granite might partly be explained by the dissolved calcium level in running water (26-35 mg/L on marl instead of levels lower than 20 mg/L on granite)., Annales Scientifiques du Limousin, Tome 26 | 2016
- Published
- 2018
- Full Text
- View/download PDF
49. Contrôle intégré de deux parasitoses basé sur l’isolement des habitats colonisés par Omphiscola glabra dans deux fermes de la Haute-Vienne
- Author
-
VIGNOLES, Philippe, Rondelaud, Daniel, and Dreyfuss, Gilles
- Subjects
biology ,Calicophoron daubneyi ,Limousin ,lymnaeid ,Omphis-cola glabra ,Forestry ,bovins ,Fasciola hepatica ,biology.organism_classification ,cattle ,limnée - Abstract
Un contrôle intégré de la fasciolose et de la paramphistomose a été appliqué pendant quatre années dans deux fermes de la Haute-Vienne afin de vérifier si la transmission de ces parasitoses pouvait être interrompue tout en assurant la sauvegarde de la limnée hôte (Omphiscola glabra). Les bovins ont été régulièrement déparasités à l’aide d’anthelminthi-ques, tandis que les habitats du mollusque ont été isolés du reste des pâtures à partir de 2011. Une troisième ferme a servi de témoin avec le déparasitage du bétail comme ci-dessus mais les biotopes de la limnée n’ont pas été clôturés. L’isolement des habitats d’O. glabra dans les deux fermes s’est traduit par une chute dans les prévalences des infestations naturelles en 2012 et la disparition des formes larvaires des parasites chez les limnées en 2013 et 2014. Chez les bovins, les prévalences ont diminué de manière progressive jusqu’à leur négativation en 2014. Dans la ferme témoin, les prévalences chez les limnées ont légèrement baissé au cours des années tandis que celles notées chez les bovins n’ont pas présenté de variation significative depuis 2011. L’isolement des habitats de cette limnée lors d’un contrôle intégré chez le bétail permet donc de sauvegarder les populations d’O. glabra tout en interrompant le cycle évolutif des deux parasites., An integrated control of fasciolosis and paramphistomosis was applied for four years in two farms of Haute Vienne to verify if transmission of these parasitoses could be interrupted and the conservation of the host snail (Omphiscola glabra) could be ensured. Cattle were regularly deparasitized using anthelminthics, while the snail habitats were isolated from the other parts of pastures since 2011. A control farm was also used with the treatment of cattle as above but snail habitats were not isolated. Fencing of snail habitats in the two farms had induced a drop in prevalences of natural infections in 2012 and disappearance of parasite larval forms from the snail bodies in 2013 and 2014. In cattle, prevalences of these infections had steadily decreased over years up to their negativation in 2014. In the control farm, prevalences in snails had only slightly decreased during the four years, while those noted in cattle did not show any significant variation since 2011. The isolation of lymnaeid habitats during the integrated control in cattle had allowed conservation of O. glabra populations and the interruption of both digenean life-cycles., Annales Scientifiques du Limousin, Tome 26 | 2016
- Published
- 2018
- Full Text
- View/download PDF
50. Decline in the number and size of Omphiscola glabra populations in northern Haute Vienne over the past thirty years
- Author
-
Dreyfuss, Gilles, VIGNOLES, Philippe, and Rondelaud, Daniel
- Subjects
densité ,Omphiscola glabra ,habitat ,population ,area ,superficie ,Lymnaeidae ,snail density - Abstract
Des investigations de terrain ont été réalisées en avril ou en mai pendant deux années (2013-2014) dans 77 fermes situées dans le nord de la Haute-Vienne pour décompter les populations d’Omphiscola glabra, déterminer la superficie de leurs habitats et préciser la densité des limnées transhivernantes par rapport aux sept types d’habitats. Les résultats ont été comparés avec ceux fournis par une première série d’investigations effectuée dans les mêmes fermes et sur les mêmes mois entre 1976 et 1984. Par rapport aux valeurs notées avant 1985, 76 % des populations (sur 54) vivant dans les jonchaies de pente et 46,5 % (sur 71) de celles qui colonisent les fossés de route ont disparu en 2013-2014. Les baisses les plus faibles ont été relevées dans les rigoles (5,6 % sur 322 avant 1985) et les fossés de drainage superficiel (8,2 % sur 49). La superficie des habitats ne présente pas de différence significative entre les deux périodes, quel que soit leur type. Par contre, les densités des mollusques transhivernants dans les habitats situés dans les fossés de route, les fossés de drainage et sur les berges d’étang sont significativement plus faibles en 2013-2014. Dans les trois autres types de gîtes, les différences entre les densités ne sont pas significatives. Ce déclin dans le nombre et la taille des populations d’O. glabra au cours des trente dernières années est principalement dû au gyrobroyage de la végétation autour des sources de pente, au curage mécanique des fossés de route ou à la réalisation d’un drainage souterrain dans les pâtures., Field investigations in 77 farms located in northern Haute-Vienne were carried out in April or May during two years (2013-2014) to count the populations of Omphiscola glabra, determine the area of their habitats, and specify the density of overwintering snails in relation to the seven types of snail habitats. The results were compared with those recorded during a first series of investigations performed between 1976 and 1984 in the same farms and during the same months. Compared to values noted before 1985, 76 % of snail populations (out of 54) living in road ditches and 46.5 % (out of 71) colonizing hillside springs have disappeared in 2013-2014. The lowest decreases were noted in open drainage furrows (5.6 % out of 322 populations before 1985) and ditches (8.2 % out of 49). The area of snail habitats did not show any significant difference between these two periods of time, whatever the type of snail habitat. In contrast, the densities of overwintering snails in 2013-2014 were significantly lower in the habitats located in road ditches, open drainage ditches and along pond banks. In the other three types of habitats, the differences between mean densities were not significant. Rotary crushing of vegetation around hillside spring heads, mechanical cleaning of road ditches, or subsurface drainage in pastures were the main causes to explain this decline in the number and size of O. glabra populations in these farms over the past thirty years., Annales Scientifiques du Limousin, Tome 26 | 2016
- Published
- 2018
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.