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1. Co-circulation of SARS-CoV-2 Alpha and Gamma variants in Italy, February and March 2021

Author

Stefanelli, P., Trentini, F., Guzzetta, G., Marziano, V., Mammone, A., Schepisi, M. S., Poletti, P., Grane, C. M., Manica, M., del Manso, M., Andrianou, X., Ajelli, M., Rezza, G., Brusaferro, S., Merler, S., Di Martino, A., Ambrosio, L., Lo Presti, A., Fiore, S., Fabiani, C., Benedetti, E., Di Mario, G., Facchini, M., Puzelli, S., Calzoletti, L., Fontana, S., Venturi, G., Fortuna, C., Marsili, G., Amendola, A., Stuppia, L., Savini, G., Picerno, A., Lopizzo, T., Dell'Edera, D., Minchella, P., Greco, F., Viglietto, G., Atripaldi, L., Limone, A., D'Agaro, P., Licastro, D., Pongolini, S., Sambri, V., Dirani, G., Zannoli, S., Affanni, P., Colucci, M. E., Capobianchi, M. R., Icardi, G., Bruzzone, B., Lillo, F., Orsi, A., Pariani, E., Baldanti, F., Molecolare, U. V., Gismondo, M. R., Maggi, F., Caruso, A., Ceriotti, F., Boniotti, M. B., Barbieri, I., Bagnarelli, P., Menzo, S., Garofalo, S., Scutella, M., Pagani, E., Collini, L., Ghisetti, V., Brossa, S., Ru, G., Bozzetta, E., Chironna, M., Parisi, A., Rubino, S., Serra, C., Piras, G., Coghe, F., Vitale, F., Tramuto, F., Scalia, G., Palermo, C. I., Mancuso, G., Pollicino, T., Di Gaudio, F., Vullo, S., Reale, S., Cusi, M. G., Rossolini, G. M., Pistello, M., Mencacci, A., Camilloni, B., Severini, S., Di Benedetto, M., Terregino, C., Monne, I., Biscaro, V., Stefanelli P, Trentini F, Guzzetta G, Marziano V, Mammone A, Sane Schepisi M, Poletti P, Molina Grané C, Manica M, Del Manso M, Andrianou X, Ajelli M, Rezza G, Brusaferro S, Merler S, Vitale F, Tramuto F, Stefanelli P., Trentini F., Guzzetta G., Marziano V., Mammone A., Sane Schepisi M., Poletti P., Molina Grane C., Manica M., Del Manso M., Andrianou X., Ajelli M., Rezza G., Brusaferro S., Merler S., Sambri V, and (membro del COVID-19 National Microbiology Surveillance Study Group)

Subjects
Epidemiology, SARS-CoV-2, Public Health, Environmental and Occupational Health, COVID-19, co-circulation, lineage, SARS-CoV-2 variant of concern, transmissibility, Humans, Italy, Models, Theoretical, Settore MED/42 - Igiene Generale E Applicata, SARS-COV-2 VARIANT OF CONCERN, CO-CIRCULATION, LINEAGE, TRANSMISSIBILITY, HUMANS, ITALY, MODELS, THEORETICAL, COVID-19, SARS-COV-2, Theoretical, Models, Virology, Human
Abstract

Background Several SARS-CoV-2 variants of concern (VOC) have emerged through 2020 and 2021. There is need for tools to estimate the relative transmissibility of emerging variants of SARS-CoV-2 with respect to circulating strains. Aim We aimed to assess the prevalence of co-circulating VOC in Italy and estimate their relative transmissibility. Methods We conducted two genomic surveillance surveys on 18 February and 18 March 2021 across the whole Italian territory covering 3,243 clinical samples and developed a mathematical model that describes the dynamics of co-circulating strains. Results The Alpha variant was already dominant on 18 February in a majority of regions/autonomous provinces (national prevalence: 54%) and almost completely replaced historical lineages by 18 March (dominant across Italy, national prevalence: 86%). We found a substantial proportion of the Gamma variant on 18 February, almost exclusively in central Italy (prevalence: 19%), which remained similar on 18 March. Nationally, the mean relative transmissibility of Alpha ranged at 1.55–1.57 times the level of historical lineages (95% CrI: 1.45–1.66). The relative transmissibility of Gamma varied according to the assumed degree of cross-protection from infection with other lineages and ranged from 1.12 (95% CrI: 1.03–1.23) with complete immune evasion to 1.39 (95% CrI: 1.26–1.56) for complete cross-protection. Conclusion We assessed the relative advantage of competing viral strains, using a mathematical model assuming different degrees of cross-protection. We found substantial co-circulation of Alpha and Gamma in Italy. Gamma was not able to outcompete Alpha, probably because of its lower transmissibility.

Published
2022

2. Characteristics of SARS-CoV-2 variants of concern B.1.1.7, B.1.351 or P.1: data from seven EU/EEA countries, weeks 38/2020 to 10/2021

Author

Funk T., Pharris A., Spiteri G., Bundle N., Melidou A., Carr M., Gonzalez G., Garcia-Leon A., Crispie F., O'Connor L., Murphy N., Mossong J., Vergison A., Wienecke-Baldacchino A. K., Abdelrahman T., Riccardo F., Stefanelli P., Di Martino A., Bella A., Lo Presti A., Casaca P., Moreno J., Borges V., Isidro J., Ferreira R., Gomes J. P., Dotsenko L., Suija H., Epstein J., Sadikova O., Sepp H., Ikonen N., Savolainen-Kopra C., Blomqvist S., Mottonen T., Helve O., Gomes-Dias J., Adlhoch C., Macori G., Russell L., Yandle Z., Bennett C., O'Byrne E., Murphy A., Tuite G., Conroy A., Duffy M., Morley U., Keoghan B., Ford I., Kennedy M., McDonnell S., Flynn A., Clarke A., Crowley A., Martin C., Kelly E., Foxton J., Hare D., Dunford L., Connell J., Moran J., Dean J., Fanning S., Rajan L., De Gascun C., Kenny J., Cotter P., Walsh C., Lawton E., Fitzpatrick A., Mullins E., Della Bartola M., McCabe M., Stapleton P., Meaney C., Fanning L., Prentice M., MacSharry J., Dempsey C., Mallon P., Leon A., Chaturvedi A., Coughlan S., McAndrew G., Reddington K., Walsh F., Fitzpatrick D., Smyth C., O'Dwyer T., Chambers T., Clarke L., Jebb D., Klopp J., Kavanagh D., Haslam K., Buckley P., Lemass K., Fitzpatrick F., Burns K., Cafferkey J., Richmond A., Foley M., Sanchez-Morgado J., Chalapati S., Pinnamaneni N., Crosbie C., Limbachiya D., Tinago W., Garcia Leon A. A., Miles S., Alalwan D., Negi R., Macken A., Feeney E., Kenny G., McCann K., Kelly N., Blair M., McCann R., Kenny C., O'Brion C., Waqas S., Savinelli S., Doran P., Bracken T., Varghese P., Lambert J. S., Cotter A., Muldoon E., Sheehan G., McGinty T., Lambert J., Green S., Leamy K., de Barra E., McConkey S., Kelly C., Horgan M., Sadlier C., Yousif O., O'Donnell J., Fitzgerald M., Petty-Saphon N., Cuddihy J., Fiore S., Fabiani C., Benedetti E., Di Mario G., Facchini M., Puzelli S., Calzoletti L., Fontana S., Venturi G., Fortuna C., Marsili G., Amendola A., Stuppia L., Savini G., Picerno A., Lopizzo T., Dell'Edera D., Minchella P., Greco F., Mauro M. V., Viglietto G., Atripaldi L., Limone A., D'Agaro P., Licastro D., Marcello A., Capobianchi M. R., Icardi G., Bruzzone B., Lillo F., Orsi A., Pariani E., Baldanti F., Gismondo M. R., Maggi F., Caruso A., Ceriotti F., Boniotti B., Bagnarelli P., Garofalo S., Scutella M., Pagani E., Collini L., Ghisetti V., Ru G., Chironna M., Parisi A., Rubino S., Serra C., Piras G., Coghe F., Vitale F., Tramuto F., Scalia G., Palermo C. I., Mancuso G., Di Gaudio F., Vullo S., Reale S., Cusi M. G., Rossolini G. M., Pistello M., Mencacci A., Camilloni B., Severini S., Di Benedetto M., Calogero T., Monne I., Biscaro V., COVID Study Groups, Funk T., Pharris A., Spiteri G., Bundle N., Melidou A., Carr M., Gonzalez G., Garcia-Leon A., Crispie F., O'Connor L., Murphy N., Mossong J., Vergison A., Wienecke-Baldacchino A.K., Abdelrahman T., Riccardo F., Stefanelli P., Di Martino A., Bella A., Lo Presti A., Casaca P., Moreno J., Borges V., Isidro J., Ferreira R., Gomes J.P., Dotsenko L., Suija H., Epstein J., Sadikova O., Sepp H., Ikonen N., Savolainen-Kopra C., Blomqvist S., Mottonen T., Helve O., Gomes-Dias J., Adlhoch C., Macori G., Russell L., Yandle Z., Bennett C., O'Byrne E., Murphy A., Tuite G., Conroy A., Duffy M., Morley U., Keoghan B., Ford I., Kennedy M., McDonnell S., Flynn A., Clarke A., Crowley A., Martin C., Kelly E., Foxton J., Hare D., Dunford L., Connell J., Moran J., Dean J., Fanning S., Rajan L., De Gascun C., Kenny J., Cotter P., Walsh C., Lawton E., Fitzpatrick A., Mullins E., Della Bartola M., McCabe M., Stapleton P., Meaney C., Fanning L., Prentice M., MacSharry J., Dempsey C., Mallon P., Leon A., Chaturvedi A., Coughlan S., McAndrew G., Reddington K., Walsh F., Fitzpatrick D., Smyth C., O'Dwyer T., Chambers T., Clarke L., Jebb D., Klopp J., Kavanagh D., Haslam K., Buckley P., Lemass K., Fitzpatrick F., Burns K., Cafferkey J., Richmond A., Foley M., Sanchez-Morgado J., Chalapati S., Pinnamaneni N., Crosbie C., Limbachiya D., Tinago W., Garcia Leon A.A., Miles S., Alalwan D., Negi R., Macken A., Feeney E., Kenny G., McCann K., Kelly N., Blair M., McCann R., Kenny C., O'Brion C., Waqas S., Savinelli S., Doran P., Bracken T., Varghese P., Lambert J.S., Cotter A., Muldoon E., Sheehan G., McGinty T., Lambert J., Green S., Leamy K., de Barra E., McConkey S., Kelly C., Horgan M., Sadlier C., Yousif O., O'Donnell J., Fitzgerald M., Petty-Saphon N., Cuddihy J., Fiore S., Fabiani C., Benedetti E., Di Mario G., Facchini M., Puzelli S., Calzoletti L., Fontana S., Venturi G., Fortuna C., Marsili G., Amendola A., Stuppia L., Savini G., Picerno A., Lopizzo T., Dell'Edera D., Minchella P., Greco F., Mauro M.V., Viglietto G., Atripaldi L., Limone A., D'Agaro P., Licastro D., Marcello A., Capobianchi M.R., Icardi G., Bruzzone B., Lillo F., Orsi A., Pariani E., Baldanti F., Gismondo M.R., Maggi F., Caruso A., Ceriotti F., Boniotti B., Bagnarelli P., Garofalo S., Scutella M., Pagani E., Collini L., Ghisetti V., Ru G., Chironna M., Parisi A., Rubino S., Serra C., Piras G., Coghe F., Vitale F., Tramuto F., Scalia G., Palermo C.I., Mancuso G., Di Gaudio F., Vullo S., Reale S., Cusi M.G., Rossolini G.M., Pistello M., Mencacci A., Camilloni B., Severini S., Di Benedetto M., Calogero T., Monne I., Biscaro V., and COVID Study Groups

Subjects
Infecções Respiratórias, 2019-20 coronavirus outbreak, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Critical Care, Epidemiology, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), variants of concern, Settore MED/42 - Igiene Generale E Applicata, 03 medical and health sciences, 0302 clinical medicine, Virology, Internal medicine, Medicine, Humans, Intensive care admission, 030212 general & internal medicine, COVID-19, Europe, SARS-CoV-2, surveillance, Surveillance, business.industry, 030503 health policy & services, Public Health, Environmental and Occupational Health, Odds ratio, Confidence interval, Variants of Concern, COVID-19, Europe, SARS-CoV-2, surveillance, variants of concern, 0305 other medical science, business, Rapid Communication, Human
Abstract

COVID study groups - PORTUGAL: Portuguese Laboratory Network for the Diagnosis of COVID-19 and Public Health Department of the Health Administrative Regions, Physicians that provided data and samples from suspected cases and SARS-CoV-2 genetic characterization. INSA laboratory team for the diagnosis of SARS-CoV-2. Algarve Biomedical Center and Unilabs. We compared 19,207 cases of SARS-CoV-2 variant B.1.1.7/S gene target failure (SGTF), 436 B.1.351 and 352 P.1 to non-variant cases reported by seven European countries. COVID-19 cases with these variants had significantly higher adjusted odds ratios for hospitalisation (B.1.1.7/SGTF: 1.7, 95% confidence interval (CI): 1.0-2.9; B.1.351: 3.6, 95% CI: 2.1-6.2; P.1: 2.6, 95% CI: 1.4-4.8) and B.1.1.7/SGTF and P.1 cases also for intensive care admission (B.1.1.7/SGTF: 2.3, 95% CI: 1.4-3.5; P.1: 2.2, 95% CI: 1.7-2.8). ECDC internal funds. The ICSC and the AIID Cohort are supported by Science Foundation Ireland under the Science Foundation Ireland, Enterprise Ireland, IDA Ireland COVID-19 Rapid Response Funding Call (Grant number: COVID-RRC 20/COV/0103 and COVID-RRC 20/COV/0305). info:eu-repo/semantics/publishedVersion

Published
2021

9. Patologia Generale

Author

Moncharmont, B., Sm, aloji s. m., Fs, ambesi impiombato f. s., Andò, S., Beguinot, F., Bifulco, M., Bonofiglio, D., Catalano, S., Chiariotti, L., Curcio, F., de marco, C., di jeso, B., Formisano, S., Gazzerro, P., Gentile, F., Graciotti, L., Leonardi, L., Malanga, D., Melillo, R., Messina, A., Olivieri, F., Pompella, A., Ad, procopio a. d., Cem, pucillo c. e. m., Quaglino, D., Ronchetti, I., Sisci, D., La, stiva l. a., Teti, D., Traverso, N., Vannini, V., Vecchio, G., Viglietto, G., Villone, G., CASTORIA, Gabriella, COLUCCI D'AMATO, Generoso Luca, GRIECO, Michele, NIGRO, Vincenzo, Moncharmont et al., Moncharmont B., Moncharmont, B., Sm, aloji s. m., Fs, ambesi impiombato f. s., Andò, S., Beguinot, F., Bifulco, M., Bonofiglio, D., Castoria, Gabriella, Catalano, S., Chiariotti, L., COLUCCI D'AMATO, Generoso Luca, Curcio, F., de marco, C., di jeso, B., Formisano, S., Gazzerro, P., Gentile, F., Graciotti, L., Grieco, Michele, Leonardi, L., Malanga, D., Melillo, R., Messina, A., Nigro, Vincenzo, Olivieri, F., Pompella, A., Ad, procopio a. d., Cem, pucillo c. e. m., Quaglino, D., Ronchetti, I., Sisci, D., La, stiva l. a., Teti, D., Traverso, N., Vannini, V., Vecchio, G., Viglietto, G., and Villone, G.

Published
2012

11. RILIEVI ANATOMO-ISTOPATOLOGICI IN TOPI P27 KNOCK-IN T197A

Author

IOVANE, VALENTINA, TRAPANI, FRANCESCA, PACIELLO, ORLANDO, PAPPARELLA, SERENELLA, De Marco C., Forzati F., Scarfò M., FUSCO, ALFREDO, Viglietto G., G. de Vico, Iovane, Valentina, De Marco, C., Forzati, F., Scarfò, M., Fusco, Alfredo, Trapani, Francesca, Paciello, Orlando, Viglietto, G., and Papparella, Serenella

Subjects
TOPI P27 KNOCK-IN T197A, chinasi ciclina-dipendenti, fenotipizzazione
Published
2009

13. Loss of tumour suppressor gene PTEN marks the transition from intratubular germ cell neoplasias (ITGCN) to invasive germ cell tumors

Author

DI VIZIO D., CITO L., BOCCIA A., INSABATO L., PETTINATO G., MOTTI M. L., SCHEPIS F., D, AMICO W., FABIANI F., TAVERNISE B., VENUTA S., FUSCO A., VIGLIETTO G., CHIEFFI, Paolo, DI VIZIO, D., Cito, L., Boccia, A., Chieffi, Paolo, Insabato, L., Pettinato, G., Motti, M. L., Schepis, F., D, Amico, W., Fabiani, F., Tavernise, B., Venuta, S., Fusco, A., and Viglietto, G.

Published
2005

14. Complex regulation of the cyclin-dependent kinase inhibitor p27kip1 in thyroid cancer cells by the PI3K/AKT pathway: regulation of p27kip1 expression and localization

Author

Motti, M. L., Califano, D., Troncone, G., Marco, C., Migliaccio, I., Palmieri, E., Pezzullo, L., Palombini, L., Alfredo Fusco, Viglietto, G., Motti, Ml, Califano, D, Troncone, Giancarlo, DE MARCO, C, Migliaccio, I, Palmieri, E, Pezzullo, L, Palombini, Lucio, Fusco, Alfredo, and Viglietto, G.

Subjects
Cytoplasm, Time Factors, Morpholines, Blotting, Western, Cell Cycle Proteins, Protein Serine-Threonine Kinases, Transfection, Phosphatidylinositol 3-Kinases, Cytosol, Cell Line, Tumor, Proto-Oncogene Proteins, Humans, Thyroid Neoplasms, Phosphorylation, S-Phase Kinase-Associated Proteins, Reverse Transcriptase Polymerase Chain Reaction, Tumor Suppressor Proteins, PTEN Phosphohydrolase, Flow Cytometry, Phosphoric Monoester Hydrolases, Androstadienes, Enzyme Activation, Gene Expression Regulation, Neoplastic, Original Research Paper, Bromodeoxyuridine, Microscopy, Fluorescence, Chromones, Wortmannin, Proto-Oncogene Proteins c-akt, Cyclin-Dependent Kinase Inhibitor p27
Abstract

Functional inactivation of the tumor suppressor p27(kip1) in human cancer occurs either through loss of expression or through phosphorylation-dependent cytoplasmic sequestration. Here we demonstrate that dysregulation of the PI3K/AKT pathway is important in thyroid carcinogenesis and that p27(kip1) is a key target of the growth-regulatory activity exerted by this pathway in thyroid cancer cells. Using specific PI3K inhibitors (LY294002, wortmannin, and PTEN) and a dominant active AKT construct (myrAKT), we demonstrated that the PI3K/AKT pathway controlled thyroid cell proliferation by regulating the expression and subcellular localization of p27. Results obtained with phospho-specific antibodies and with transfection of nonphosphorylable p27(kip1) mutant constructs demonstrated that PI3K/AKT-dependent regulation of p27(kip1) mislocalization in thyroid cancer cells occurred via phosphorylation of p27(kip1) at T157 and T198 (but not at S10 or T187). Finally, we evaluated whether these results were applicable to human tumors. Analysis of 100 thyroid carcinomas indicated that p27(kip1) phosphorylation at T157/T198 and cytoplasmic mislocalization were preferentially associated with activation of the PI3K/AKT pathway. Thus the PI3/AKT pathway and its effector p27(kip1) play major roles in thyroid carcinogenesis.

Published
2005

15. HMGA1 protein overexpression is a frequent feature of epithelial ovarian carcinomas

Author

MASCIULLO V, BALDASSARRE G, PENTIMALLI F, BERLINGIERI MT, BOCCIA A, CHIAPPETTA G, PALAZZO J, VIGLIETTO G, SCAMBIA G, AND FUSCO A., MANFIOLETTI, GUIDALBERTO, GIANCOTTI, VINCENZO, Masciullo, V, Baldassarre, G, Pentimalli, F, Berlingieri, Mt, Boccia, A, Chiappetta, G, Palazzo, J, Manfioletti, Guidalberto, Giancotti, Vincenzo, Viglietto, G, Scambia, G, and AND FUSCO, A.

Published
2003

16. An adenovirus carrying the rat protein tyrosine phosphatase eta suppresses the growth of human thyroid carcinoma cell lines in vitro and in vivo

Author

Iuliano, R., Trapasso, F., Le Pera, I., filippo schepis, Sama, I., Clodomiro, A., Dumon, K. R., Santoro, M., Chiariotti, L., Viglietto, G., Fusco, A., Iuliano, R, Trapasso, F, LE PERA, I, Schepis, F, Sama, I, Clodomiro, A, Dumon, Kr, Santoro, Massimo, Chiariotti, Lorenzo, Viglietto, G, and Fusco, Alfredo

Subjects
EXPRESSION, PHOSPHOLIPASE C-GAMMA, Genetic Vectors, Mice, Nude, Cell Cycle Proteins, THERAPY, Adenoviridae, Mice, Adenocarcinoma, Follicular, Animals, Cell Division, Cyclin-Dependent Kinase Inhibitor p27, Gene Therapy, Humans, Isoenzymes, Nude, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases, Phospholipase C gamma, Phosphorylation, Protein Tyrosine Phosphatases, Rats, Thyroid Neoplasms, Transduction, Genetic, Tumor Cells, Cultured, Tumor Suppressor Proteins, Type C Phospholipases, Xenograft Model Antitumor Assays, Transduction, Genetic, Adenocarcinoma, Follicular, Tumor Cells, Cultured, DEP-1, Genetic Therapy
Abstract

We demonstrated previously that rat tyrosine phosphatase r-PTPeta expression was suppressed in rat and human thyroid neoplastic cells, and that restoration of r-PTPeta expression reverted the malignant phenotype. To investigate the potential of this gene for cancer therapy, we generated an adenovirus carrying the r-PTPeta cDNA (Ad-r-PTPeta). This virus infected human thyroid carcinoma cells and overexpressed the r-PTPeta protein. Overexpression of r-PTPeta significantly inhibited the growth of four thyroid carcinoma cell lines. Cell growth inhibition was associated with down-regulation of extracellular signal-regulated kinase 1/2 activity, with increased levels of the cell-cycle inhibitor p27(kip1) protein and with dephosphorylation of PLCgamma1, a substrate of DEP-1, the human homologue of r-PTPeta. Finally, the growth of xenograft tumors induced in athymic mice by anaplastic thyroid carcinoma ARO cells transduced with the Ad-r-PTPeta virus was drastically reduced. These data suggest that gene therapy based on restoration of PTPeta function has potential in the treatment of human thyroid malignant neoplasias.

Published
2003

18. Induction of ETS-1 and ETS-2 transcription factors is required for thyroid cell transformation

Author

Nigris, F., Mega, T., Berger, N., Barone, M. V., Santoro, M., Viglietto, G., Verde, P., Alfredo Fusco, de NIGRIS, Filomena, Mega, T, Berger, N, Barone, Mv, Santoro, M, Viglietto, G, Verde, P, Fusco, A., F., de Nigri, T., Mega, N., Berger, Barone, MARIA VITTORIA, Santoro, Massimo, G., Viglietto, P., Verde, and Fusco, Alfredo

Subjects
Transcriptional Activation, Proto-Oncogene Proteins c-ets, Genes, myc, Thyroid Gland, Apoptosis, DNA, Proto-Oncogene Protein c-ets-2, DNA-Binding Proteins, Proto-Oncogene Protein c-ets-1, Repressor Proteins, Cell Transformation, Neoplastic, Proto-Oncogene Proteins, Consensus Sequence, Trans-Activators, Tumor Cells, Cultured, Humans, Thyroid Neoplasms, Transcription Factors
Abstract

The proteins of the Ets family are transcription factors involved in signal transduction, cell cycle progression, and differentiation. In this study, we report that thyroid cell neoplastic transformation is associated with a dramatic increase in ETS transcriptional activity, which is dependent on the accumulation of Ets-1, Ets-2, and other Ets-related proteins. Inhibition of ETS transactivation activity by the Ets-dominant negative construct (Ets-Z) induced programmed cell death in human thyroid carcinoma cell lines but not in normal thyroid cells. Apoptotic cell death induced by Ets-Z was dependent on the reduction of c-MYC protein levels, because it was prevented by overexpressinn of c-myc. Taken together, these data indicate that the induction of Ets-l and Ets-2 transcription factors plays a pivotal role in thyroid cell neoplastic transformation.

Published
2001

19. RNF4 is a growth inhibitor expressed in germ cells and lost in human testicular tumours

Author

PERO R., LEMBO F., DI VIZIO D., BOCCIA A., FEDELE M., PIERANTONI M. G., ROSSI P., IULIANO R., SANTORO M., VIGLIETTO G., BRUNI C. B., FUSCO A., CHIARIOTTI L., CHIEFFI, Paolo, Pero, R., Lembo, F., DI VIZIO, D., Boccia, A., Chieffi, Paolo, Fedele, M., Pierantoni, M. G., Rossi, P., Iuliano, R., Santoro, M., Viglietto, G., Bruni, C. B., Fusco, A., and Chiariotti, L.

Published
2001

20. PATOLOGIA GENERALE

Author

Moncharmont, Bruno, Aloj, S. M., AMBESI IMPIOMBATO, F. S., Beguinot, S. ANDÒ F., Bifulco, M., Bonofiglio, D., Catalano, G. CASTORIA S., Chiariotti, L., COLUCCI D’AMATO, L., DE MARCO, F. CURCIO C., DI JESO, B., Formisano, S., Gazzerro, P., Graciotti, F. GENTILE L., Grieco, M., Leonardi, A., Malanga, D., Messina, R. MELILLO A., Nigro, V., Olivieri, F., Pompella, A., Pucillo, A. D. PROCOPIO C. E. M., Quaglino, D., Ronchetti, I., Sisci, D., Teti, L. A. STIVALA D., Traverso, N., Vannini, V., Vecchio, G., Viglietto, G., and Villone, G.

Published
2012

22. Akt/PKB promotes survival and hormone-independent proliferation of thyroid cells in the absence of de-differentiated and transforming effects

Author

DE VITA, GABRIELLA, BERLINGIERI M. T., VISCONTI R., CASTELLONE M. D., VIGLIETTO G., BALDASSARRE G., BELLACOSA A., TSICHLIS P. M., FUSCO A., SANTORO M., ZANNINI, MARIASTELLA, DE VITA, Gabriella, Berlingieri, M. T., Visconti, R., Castellone, M. D., Viglietto, G., Baldassarre, G., Zannini, Mariastella, Bellacosa, A., Tsichlis, P. M., Fusco, A., and Santoro, M.

Published
2000

23. Homeodomain-interacting protein kinase-2 stabilizes p27(kip1) by its phosphorylation at serine 10 and contributes to cell motility

Author

Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, and Fusco A.

Abstract

HIPK2 is a serine/threonine kinase that acts as a coregulator of an increasing number of factors involved in cell survival and proliferation during development and in response to different types of stress. Here we report on a novel target of HIPK2, the cyclin-dependent kinase inhibitor p27(kip1). HIPK2 phosphorylates p27(kip1) in vitro and in vivo at serine 10, an event that accounts for 80% of the total p27(kip1) phosphorylation and plays a crucial role in the stability of the protein. Indeed, HIPK2 depletion by transient or stable RNA interference in tumor cells of different origin was consistently associated with strong reduction of p27(kip1) phosphorylation at serine 10 and of p27(kip1) stability. An initial evaluation of the functional relevance of this HIPK2-mediated regulation of p27(kip1) revealed a contribution to cell motility, rather than to cell proliferation, but only in cells that do not express wild-type p53.

Published
2011

24. High prevalence of RET/PTC rearrangements in Ukrainian and Belarussia post-Chernobyl thyroid papillary carcinomas: a strong correlation between RET/PTC3 and the solid-follicular variant

Author

Thomas, G. A., Bunnell, H., Cook, H. A., Williams, E. D., Nerovnya, A., Cherstvoy, E. D., Tronko, N. D., Tetiana Bogdanova, Chiappetta, G., Viglietto, G., Pentimalli, F., Salvatore, G., Fusco, A., Santoro, M., Vecchio, G., G. A., Thoma, Bunnel, H., Cook, H. A., Williams, E. D., Nerovnya, A., Cherstvoy, E. D., Tronko, N. D., Bogdanova, T. I., Chiappetta, G., Viglietto, G., Pentimalli, F., Salvatore, Giuliana, Fusco, A., Santoro, M., Vecchio, Giancarlo, H., Bunnell, H. A., Cook, E. D., William, A., Nerovnya, E. D., Cherstvoy, N. D., Tronko, T. I., Bogdanova, G., Chiappetta, G., Viglietto, F., Pentimalli, G., Salvatore, Fusco, Alfredo, and Santoro, Massimo

Subjects
genetics, Child, Drosophila Proteins, Female, Gene Rearrangement, Humans, Male, Power Plants, Proto-Oncogene Proteins c-ret, Proto-Oncogene Protein, Adolescent, Carcinoma, Papillary, genetics, Radioactive Hazard Release, Receptor Protein-Tyrosine Kinase, genetics, Ukraine, genetics, Republic of Belarus, Reverse Transcriptase Polymerase Chain Reaction, Thyroid Neoplasm
Abstract

A sharp increase in the incidence of pediatric thyroid papillary cancer was documented after the Chernobyl power plant explosion. An increased prevalence of rearrangements of the RET protooncogene (RET/PTC rearrangements) has been reported in Belarussian post-Chernobyl papillary carcinomas arising between 1990 and 1995. We analyzed 67 post-Chernobyl pediatric papillary carcinomas arising in 1995-1997 for RET/PTC activation: 28 were from Ukraine and 39 were from Belarus. The study, conducted by a combined immunohistochemistry and RT-PCR approach, demonstrated a high frequency (60.7\% of the Ukrainian and 51.3\% of the Belarussian cases) of RET/PTC activation. A strong correlation was observed between the solid-follicular subtype of papillary carcinoma and the RET/PTC3 isoform: 19 of the 24 RET/PTC-positive solid-follicular carcinomas harbored a RET/PTC3 rearrangement, whereas only 5 had a RET/PTC1 rearrangement. Taken together these results support the concept that RET/PTC activation plays a central role in the pathogenesis of thyroid papillary carcinomas in both Ukraine and Belarus after the Chernobyl accident.

Published
1999

27. Human colorectal carcinomas express high levels of High Mobility Group HMGI(Y) proteins

Author

Monica Fedele, Bandiera, A., Chiappetta, G., Battista, S., Viglietto, G., Manfioletti, G., Casamassimi, A., Santoro, M., Giancotti, V., Fusco, A., Fedele, M, Bandiera, Antonella, Chiappetta, G, Battista, S, Viglietto, G, Manfioletti, Guidalberto, Casamassimi, A, Santoro, M, Giancotti, V, Fusco, A., M., Fedele, A., Bandiera, G., Chiappetta, S., Battista, G., Viglietto, G., Manfioletti, A., Casamassimi, Santoro, Massimo, V., Giancotti, and Fusco, Alfredo

Subjects
Male, Colon, Molecular Sequence Data, metabolism, Thyroid Neoplasm, Thyroid Gland, Gene Expression, Amino Acid Sequence, Animals, Antibodies, Cell Line, Colon, analysis/biosynthesis, Humans, Immunohistochemistry, Male, Molecular Sequence Data, Peptide Fragment, Antibodies, Epithelium, Cell Line, metabolism/pathology, Tumor Cell, Reference Values, Tumor Cells, Cultured, Animals, Humans, Amino Acid Sequence, HMGA1a Protein, Thyroid Neoplasms, metabolism, Female, Fibroblast, metabolism/pathology/surgery, Colorectal Neoplasm, metabolism/pathology, Epithelium, Cultured, cytology/metabolism/pathology, Colonic Neoplasm, High Mobility Group Proteins, Fibroblasts, Immunohistochemistry, Peptide Fragments, Rats, chemical synthesis/immunology, Rats, Reference Values, Thyroid Gland, metabolism, Gene Expression, HMGA1a Protein, High Mobility Group Protein, Colonic Neoplasms, Female, Colorectal Neoplasms
Abstract

A correlation has previously been demonstrated between the presence of the three HMGI proteins (HMGI, HMGY, and HMGI-C) and the expression of a highly malignant phenotype in epithelial and fibroblastic rat thyroid cells; this being subsequently extended to experimental thyroid, lung, prostate, mammary, and skin carcinomas. Recently, we have demonstrated that expression of HMGI and HMGY proteins, coded for by the HMGI(Y) gene, is associated with the malignant phenotype of human thyroid neoplasias. Here, we show that HMGI(Y) gene expression is present both at the RNA and protein level in human colorectal carcinoma cell lines and tissues examined in this study. Conversely, no HMGI(Y) proteins were detected in normal intestinal mucosa. Therefore, these results suggest an involvement of HMGI and HMGY proteins overexpression in colorectal tumorigenesis.

Published
1996

35. Adult c-kitpos cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair

Author

Ellison, G, Vicinanza, C, Smith, A, Aquila, I, Leone, A, Waring, C, Henning, B, Stirparo, G, Papait, R, Scarfò, M, Agosti, V, Viglietto, G, Condorelli, G, Indolfi, C, Ottolenghi, S, Torella, D, Nadal Ginard, B, Nadal Ginard, B., CONDORELLI, GIANLUIGI, OTTOLENGHI, SERGIO, Ellison, G, Vicinanza, C, Smith, A, Aquila, I, Leone, A, Waring, C, Henning, B, Stirparo, G, Papait, R, Scarfò, M, Agosti, V, Viglietto, G, Condorelli, G, Indolfi, C, Ottolenghi, S, Torella, D, Nadal Ginard, B, Nadal Ginard, B., CONDORELLI, GIANLUIGI, and OTTOLENGHI, SERGIO

Abstract

The epidemic of heart failure has stimulated interest in understanding cardiac regeneration. Evidence has been reported supporting regeneration via transplantation of multiple cell types, as well as replication of postmitotic cardiomyocytes. In addition, the adult myocardium harbors endogenous c-kit pos cardiac stem cells (eCSCs), whose relevance for regeneration is controversial. Here, using different rodent models of diffuse myocardial damage causing acute heart failure, we show that eCSCs restore cardiac function by regenerating lost cardiomyocytes. Ablation of the eCSC abolishes regeneration and functional recovery. The regenerative process is completely restored by replacing the ablated eCSCs with the progeny of one eCSC. eCSCs recovered from the host and recloned retain their regenerative potential in vivo and in vitro. After regeneration, selective suicide of these exogenous CSCs and their progeny abolishes regeneration, severely impairing ventricular performance. These data show that c-kitpos eCSCs are necessary and sufficient for the regeneration and repair of myocardial damage. © 2013 Elsevier Inc.

Published
2013

36. Critical role of the HMGI(Y) proteins in adipocytic cell growth and differentiation

Author

Melillo R.M., Pierantoni G.M., Scala S., Battista S., Fedele M., Stella A., De Biasio M.C., Chiappetta G., Fidanza V., Condorelli G., Santoro M., Croce C.M., Viglietto G., and Fusco A.

Abstract

The high-mobility group I (HMGI) nonhistone chromosomal proteins HMGI(Y) and HMGI-C have been implicated in defining chromatin structure and in regulating the transcription of several genes. These proteins have been implicated in adipocyte homeostasis: a severe deficiency of fat tissue is found in mice with targeted disruption of the HMGI-C locus, and lipomagenesis in humans is frequently associated with somatic mutations of HMGI genes. The aim of this study was to examine the role of HMGI(Y) proteins in adipocytic cell growth and differentiation. First, we found that differentiation of the preadipocytic 3T3-L1 cell line caused early induction of HMGI(Y) gene expression. Suppression of HMGI(Y) expression by antisense technology dramatically increased the growth rate and impaired adipocytic differentiation in these cells. The process of adipogenic differentiation involves the interplay of several transcription factors, among which is the CCAAT/enhancer-binding protein (C/EBP) family of proteins. These factors are required for the transcriptional activation of adipocyte-specific genes. We also tested the hypothesis that HMGI(Y) might participate in transcriptional control of adipocyte-specific promoters. We found that HMGI(Y) proteins bind C/EBPbeta in vivo and in vitro. Furthermore, we show that HMGI(Y) strongly potentiates the capacity of C/EBPbeta to transactivate the leptin promoter, an adipose-specific promoter. Taken together, these results indicate that the HMGI(Y) proteins play a critical role in adipocytic cell growth and differentiation.

Published
2001

37. Retinoic acid induces neuronal differentiation of embryonal carcinoma cells by reducing proteasome-dependent proteolysis of the cyclin-dependent inhibitor p27

Author

Baldassarre, G., Boccia, A., Bruni, P., Sandomenico, C., Maria Vittoria BARONE, Pepe, S., Angrisano, T., Belletti, B., Motti, M. L., Fusco, A., Viglietto, G., G., Baldassarre, A., Boccia, P., Bruni, C., Sandomenico, M. V., Barone, S., Pepe, Angrisano, Tiziana, B., Belletti, M. L., Motti, Fusco, Alfredo, G., Viglietto, Barone, MARIA VITTORIA, Pepe, Stefano, and G. V. i. g. l. i. e. t. t., O.

Subjects
Neurons, Proteasome Endopeptidase Complex, Tumor Suppressor Proteins, p27, Antineoplastic Agents, Cell Cycle Proteins, Cell Differentiation, Tretinoin, NTERA-2 clone D1 cell line, Cyclin-Dependent Kinases, Gene Expression Regulation, Neoplastic, Cysteine Endopeptidases, Multienzyme Complexes, Carcinoma, Embryonal, Tumor Cells, Cultured, retinoic acid, Humans, Microtubule-Associated Proteins, neuronal differentiation, Cyclin-Dependent Kinase Inhibitor p27
Abstract

Retinoic acid (RA) treatment of embryonal carcinoma cell line NTERA-2 clone D1 (NT2/D1) induces growth arrest and terminal differentiation along the neuronal pathway. In the present study, we provide a functional link between RA and p27 function in the control of neuronal differentiation in NT2/D1 cells. We report that RA enhances p27 expression, which results in increased association with cyclin E/cyclin-dependent kinase 2 complexes and suppression of their activity; however, antisense clones, which have greatly reduced RA-dependent p27 inducibility (NT2-p27AS), continue to synthesize DNA and are unable to differentiate properly in response to RA as determined by lack of neurite outgrowth and by the failure to modify surface antigens. As to the mechanism involved in RA-dependent p27 up-regulation, our data support the concept that RA reduces p27 protein degradation through the ubiquitin/proteasome-dependent pathway. Taken together, these findings demonstrate that in embryonal carcinoma cells, p27 expression is required for growth arrest and proper neuronal differentiation.

Published
2000

38. The placenta growth factor gene of the mouse

Author

DI PALMA, T, Tucci, M, Russo, G, Maglione, D, Lago, Ct, Romano, A, Saccone, Salvatore, DELLA VALLE, G, DE GREGORIO, L, Dragani, T, and Viglietto, G. AND PERSICO M. G.

Published
1996

39. VEGF and PlGF

Author

Vincenti, V, Lago, Ct, DI PALMA, T, Tucci, M, Maglione, D, Russo, G, DE FELICE, M, Parise, G, Saccone, Salvatore, DELLA VALLE, G, DE GREGORIO, L, Dragani, T, Rocchi, M, Paoletti, I, Romano, A, Ippolito, P, and AND PERSICO MG, VIGLIETTO G.

Published
1995

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