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8 results on '"Vieira Branquinho, Mariana"'

4. Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injury

Author

Pinho, Ana Catarina, primary, Vieira Branquinho, Mariana, additional, Alvites, Rui Damásio, additional, Fonseca, Ana Clotilde, additional, Caseiro, Ana Rita, additional, Santos Pedrosa, Sílvia, additional, Luís, Ana Lúcia, additional, Pires, Isabel, additional, Prada, Justina, additional, Muratori, Luísa, additional, Ronchi, Giulia, additional, Geuna, Stefano, additional, Santos, José Domingos, additional, Maurício, Ana Colette, additional, Serra, Arménio Coimbra, additional, and Coelho, Jorge Fernando Jordão, additional

Published
2020
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5. Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretome

Author

Caseiro, Ana Rita, primary, Santos Pedrosa, Sílvia, additional, Ivanova, Galya, additional, Vieira Branquinho, Mariana, additional, Almeida, André, additional, Faria, Fátima, additional, Amorim, Irina, additional, Pereira, Tiago, additional, and Maurício, Ana Colette, additional

Published
2019
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7. Dextran-based tube-guides for the regeneration of the rat sciatic nerve after neurotmesis injuryElectronic supplementary information (ESI) available: Table with data from the motor performance assessment (motor deficit determined from the extensor postural thrust – EPT) over the 20 weeks recovery period (T1–T20) (Table S1); table with data from nociceptive function assessment (withdrawal reflex time – WRL) over the 20 weeks recovery period (T1–T20) (Table S2); table with data from functional recovery through the static sciatic index (SSI) over the 20 weeks recovery period (T1–T20) (Table S3). See DOI: 10.1039/c9bm00901a

Author

PinhoThese authors contributed equally., Ana Catarina, Vieira Branquinho, Mariana, Alvites, Rui Damásio, Fonseca, Ana Clotilde, Caseiro, Ana Rita, Santos Pedrosa, Sílvia, Luís, Ana Lúcia, Pires, Isabel, Prada, Justina, Muratori, Luísa, Ronchi, Giulia, Geuna, Stefano, Santos, José Domingos, Maurício, Ana Colette, Serra, Arménio Coimbra, and Coelho, Jorge Fernando Jordão

Abstract

In this work, dextran-based nerve tube-guides were prepared, characterized and used in a standardized animal model of neurotmesis injury. Non-porous and porous transparent tube-guides were obtained by photocrosslinking of two co-macromonomers based on dextran and poly(-caprolactone) (PCL). Swelling capacity of the tube-guides ranged from 40–60% with no visible constriction of their inner diameter. In vitrohydrolytic degradation tests showed that the tube-guides maintained their structural integrity up to 6 months. The in vivoperformance of the tube-guides was evaluated by entubulation of the rat sciatic nerve after a neurotmesis injury, with a 10 mm-gap between the nerve stumps. The results showed that the tube-guides were able to promote the regeneration of the nerve in a similar manner to what was observed with conventional techniques (nerve graft and end-to-end suture). Stereological analysis proved that nerve regeneration occurred, and both tube-guides presented fibre diameter and g-ratio closer to healthy sciatic nerves. The histomorphometric analysis of Tibialis anterior(TA) skeletal muscle showed decreased neurogenic atrophy in the porous tube-guides treated group, presenting measurements that are similar to the uninjured control.

Published
2020
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8. Biomaterials and Cellular Systems at the Forefront of Peripheral Nerve Regeneration

Author

Maurício, Ana Colette, Alvites, Rui Damásio, Vieira Branquinho, Mariana, Caseiro, Rita, Santos Pedrosa, Sílvia, Luís, Ana Lúcia, Geuna, Stefano, and Varejão, Artur

Subjects
Medical
Abstract

Peripheral nerve injuries remain a common clinical complication, and currently available therapies present significant limitations, often resulting in poor and suboptimal outcomes. Despite significant developments in microsurgical approaches in the last decades, no effective treatment options have been disclosed. Current research focuses on the optimization of such microsurgical techniques and on their combination with other pro-regenerative factors, such as mesenchymal stem cells or biomaterials. Mesenchymal stem cells present a remarkable capacity for bioactive molecule production that modulates inflammatory and regenerative processes, stimulating peripheral nerve regeneration. In parallel, efforts have been directed towards the development of biomaterial nerve guidance channels and nerve conduits. These biomaterials have been optimized in terms of biodegradability, ability to release bioactive factors, incorporation of cellular agents, and internal matrix architecture (to enable cellular migration and mimic native tissue morphology and to generate and bear specific electrical activity). The current literature review presents relevant advances in the development of mesenchymal stem cell and biomaterial-based therapeutic approaches aiming at the peripheral nerve tissue regeneration in diverse lesion scenarios, also exploring the advances achieved by our research group in this field in recent years.

Published
2019

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