1. Effects of green tea compound epigallocatechin-3-gallate against Stenotrophomonas maltophilia infection and biofilm
- Author
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Vidigal, Pedrina G., Müsken, Mathias, Becker, Katrin A., Häussler, Susanne, Wingender, Jost, Steinmann, Eike, Kehrmann, Jan, Gulbins, Erich, Buer, Jan, Rath, Peter-Michael, Steinmann, Jörg, and Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany
- Subjects
Cystic Fibrosis ,Stenotrophomonas maltophilia ,Medizin ,lcsh:Medicine ,Catechin ,Medizinische Fakultät » Universitätsklinikum Essen » Institut für Molekularbiologie (Tumorforschung) ,Medicine and Health Sciences ,lcsh:Science ,Fakultät für Chemie » Biofilm Center ,Ecology ,food and beverages ,Animal Models ,Infectious Diseases ,Instillation, Drug ,ddc:540 ,Female ,Genetic Dominance ,Research Article ,Medizinische Fakultät » Universitätsklinikum Essen » Institut für Medizinische Mikrobiologie ,Mouse Models ,Microbial Sensitivity Tests ,Research and Analysis Methods ,Microbiology ,complex mixtures ,Microbial Ecology ,Model Organisms ,Complementary and Alternative Medicine ,Genetics ,ddc:61 ,Animals ,ddc:610 ,Clinical Genetics ,Autosomal Recessive Traits ,Tea ,Colistin ,lcsh:R ,Biology and Life Sciences ,Human Genetics ,Bacteriology ,biochemical phenomena, metabolism, and nutrition ,Fibrosis ,Bacterial Load ,Mice, Mutant Strains ,Mice, Inbred C57BL ,Kinetics ,Biofilms ,lcsh:Q ,sense organs ,Bacterial Biofilms ,Developmental Biology - Abstract
We investigated the in vitro and in vivo activities of epigallocatechin-3- gallate (EGCg), a green tea component, against Stenotrophomonas maltophilia (Sm) isolates from cystic fibrosis (CF) patients. In vitro effects of EGCg and the antibiotic colistin (COL) on growth inhibition, survival, and also against young and mature biofilms of S. maltophilia were determined. Qualitative and quantitative changes on the biofilms were assessed by confocal laser scanning microscopy (CLSM). Further, in vivo effects of nebulized EGCg in C57BL/6 and Cftr mutant mice during acute Sm lung infection were evaluated. Subinhibitory concentrations of EGCg significantly reduced not only biofilm formation, but also the quantity of viable cells in young and mature biofilms. CLSM showed that EGCg-exposed biofilms exhibited either a change in total biofilm biovolume or an increase of the fraction of dead cells contained within the biofilm in a dose depended manner. Sm infected wild-type and Cftr mutant mice treated with 1,024 mg/L EGCg by inhalation exhibited significantly lower bacterial counts than those undergoing no treatment or treated with COL. EGCg displayed promising inhibitory and anti-biofilm properties against CF Sm isolates in vitro and significantly reduced Sm bacterial counts in an acute infection model with wild type and CF mice. This natural compound may represent a novel therapeutic agent against Sm infection in CF. \copyright 2014 Vidigal et al. OA Förderung 2014
- Published
- 2014