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Old women are expected to undergo pelvic organ prolapse (POP) surgery. Distress caused by POP affects women´s quality of life. Although polypropylene mesh (PM) is considered a good material to restore the pelvic floor, there are concerns about infection and vaginal mesh exposure. This research studies the optic properties of inflammatory reaction and the tissue repair induced by a PM covered by porcine collagen membrane (CM). Thirty female rats were shared in two groups: (A) exposed to PM covered by CM and (B) exposed to PM alone; five animals each group were euthanized at day 7, 14 and 28 of post-operation day (POD). Collagen anisotropic properties were analyzed. In the 7⍛PO group A pushes vascular proliferation on, as lymphocytes and fibroblasts infiltration. Group B evidenced amount of histiocytes and beginning of encapsulating. At the 14⍛PO group A keeps the characteristics of lymphocytic infiltration and rounding the PM there is a thin layer of fat tissue separating it from the muscles layer; Group B evidenced amount of fibroblasts rounding the PM and multinucleated giant cells crushed by a wrap of collagen and fibroblasts. The 28⍛PO group A revealed big fibroblasts process with expressive histiocytes presence. Group B shows much multinucleated giant cells trying to wrap up the polypropylene, a considerate decreased infiltration, and the striated muscles layer infiltrated the implanted area. PM covered with a highly purified porcine collagen membrane showed earlier resolution of inflammatory reaction, better neoangiogenesis and more organized host collagen deposition than in pure PM.

Agradecimentos: This work was supported by the São Paulo state Research Foundation (FAPESP, Brazil; grant no. 2015/10356-2) and the Brazilian National Council for Research and Development (CNPq; grants no. 304668/2014-1 and 421299/2018-5). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. The authors thank Mr. E.H.M. dos Anjos for assistance with formatting of the figures Abstract: Valproic acid/sodium valproate (VPA), a drug primarily used for treatment of seizure disorders, is recognized as an efficient epigenetic agent, inducing inhibition of histone deacetylases, promoting changes in the methylation status of DNA and histones, and affecting chromatin structure. In addition to these epigenetic effects, molecular affinity of VPA for histone H1 has been proposed based on thermal denaturation, fluorescence spectroscopy and circular dichroism assays. VPA interactions with DNA and histones using Fourier transform infrared (FTIR) microspectroscopy and high-performance polarization microscopy that are not related to the effects promoted on epigenetic markers have been recently explored. Data in this article provide supplementary information for a better understanding of the resulting FTIR spectra for mixtures of VPA and histones H1 and H3 and of the potential effect of VPA directly on histones that has been reported in the literature FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ Aberto

Agradecimentos: The support of Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Brazil; grant no. 2007/58251-8) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil: grant no. 304668/2014-1) is gratefully acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript Abstract: Optical anisotropy properties and infrared spectra of collagen and DNA as individual entities have been widely described. However, they have not been studied in collagen-DNA complex biofilms. We investigated whether collagen type I and DNA birefringence evaluated using polarization microscopy, and the vibrational patterns of their chemical groups identified in Fourier transform-infrared (FT-IR) spectra are retained in collagen-DNA complexes. Collagen gel rods extruded into a DNA saline solution and films dried from drops using mixtures of a 10-µL (14 µg) collagen solution and a 50-µL (305 µg) DNA solution and of a 50-µL (70 µg) collagen solution and a 10-µL (61 µg) DNA solution were used. Changes in the collagen fibers' stereoarrangement occurred in the gel rods interacting with the DNA. Even when the DNA concentration (305 µg) was significantly higher than the collagen concentration (14 µg) and the DNA played the main role in establishing the overall oriented geometry in the collagen-DNA films (intertwisted fibrillary structure and negative birefringence), some collagen markers were present in the FT-IR spectra of the complex. When collagen and DNA concentrations were similar, DNA denaturation may occur. Present observations suggest formation of collagen-DNA complexes even if highly organized collagen macromolecules are offered to DNA CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP Fechado