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2. New Approaches for Basophil Activation Tests Employing Dendrimeric Antigen–Silica Nanoparticle Composites.

Author

Calvo-Serrano, Silvia, Matamoros, Esther, Céspedes, Jose Antonio, Fernández-Santamaría, Rubén, Gil-Ocaña, Violeta, Perez-Inestrosa, Ezequiel, Frecha, Cecilia, Montañez, Maria I., Vida, Yolanda, Mayorga, Cristobalina, and Torres, Maria J.

Subjects
SILICA nanoparticles, DRUG allergy, ALLERGIES, BASOPHILS, NANOPARTICLES
Abstract

In vitro cell activation through specific IgE bound to high-affinity receptors on the basophil surface is a widely used strategy for the evaluation of IgE-mediated immediate hypersensitivity reactions to betalactams. Cellular activation requires drug conjugation to a protein to form a large enough structure displaying a certain distance between haptens to allow the cross-linking of two IgE antibodies bound to the basophil's surface, triggering their degranulation. However, no information about the size and composition of these conjugates is available. Routine in vitro diagnosis using the basophil activation test uses free amoxicillin, which is assumed to conjugate to a carrier present in blood. To standardize the methodology, we propose the use of well-controlled and defined nanomaterials functionalized with amoxicilloyl. Silica nanoparticles decorated with PAMAM–dendrimer–amoxicilloyl conjugates (NpDeAXO) of different sizes and amoxicilloyl densities (50–300 µmol amoxicilloyl/gram nanoparticle) have been prepared and chemically characterized. Two methods of synthesis were performed to ensure reproducibility and stability. Their functional effect on basophils was measured using an in-house basophil activation test (BAT) that determines CD63+ or CD203chigh activation markers. It was observed that NpDeAXO nanocomposites are not only able to specifically activate basophils but also do so in a more effective way than free amoxicillin, pointing to a translational potential diagnosis. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Substrate adhesion determines migration during mesenchymal cell condensation in

Author

Casanellas, Ignasi, Jiang, Hongkai, David, Carolyn M., Vida, Yolanda, Perez-Inestrosa, Ezequiel, Samitier, Josep, Lagunas, Anna, [Casanellas, Ignasi] Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanobioengn Grp, Barcelona 08028, Spain, [Samitier, Josep] Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanobioengn Grp, Barcelona 08028, Spain, [Lagunas, Anna] Barcelona Inst Sci & Technol BIST, Inst Bioengn Catalonia IBEC, Nanobioengn Grp, Barcelona 08028, Spain, [Casanellas, Ignasi] Univ Barcelona UB, Fac Phys, Dept Elect & Biomed Engn, Barcelona 08028, Spain, [Samitier, Josep] Univ Barcelona UB, Fac Phys, Dept Elect & Biomed Engn, Barcelona 08028, Spain, [Casanellas, Ignasi] Biomed Res Networking Ctr Bioengn Biomat & Nanome, Madrid 28029, Spain, [Samitier, Josep] Biomed Res Networking Ctr Bioengn Biomat & Nanome, Madrid 28029, Spain, [Lagunas, Anna] Biomed Res Networking Ctr Bioengn Biomat & Nanome, Madrid 28029, Spain, [Jiang, Hongkai] Vanderbilt Univ, Sch Engn, Biomed Engn, Nashville, TN 37235 USA, [David, Carolyn M.] Lehigh Univ, PC Rossin Coll Engn & Appl Sci, Bethlehem, PA 18015 USA, [Vida, Yolanda] Univ Malaga IBIMA, Dept Quim Organ, Campus Teatinos, Malaga 29071, Spain, [Perez-Inestrosa, Ezequiel] Univ Malaga IBIMA, Dept Quim Organ, Campus Teatinos, Malaga 29071, Spain, [Vida, Yolanda] Ctr Andaluz Nanomed & Biotecnol BIONAND, Lab Dendrimers Biomimet & Photon, Parque Tecnol Andalucia, Malaga 29590, Spain, [Perez-Inestrosa, Ezequiel] Ctr Andaluz Nanomed & Biotecnol BIONAND, Lab Dendrimers Biomimet & Photon, Parque Tecnol Andalucia, Malaga 29590, Spain, [Casanellas, Ignasi] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA, Biomedical Research Networking Center (Centro de Investigacion Biomedica en Red, CIBER), Spain, VI National RDi Plan, Iniciativa Ingenio, Consolider Program, CIBER Actions, Instituto de Salud Carlos III, European Regional Development Fund (ERDF), Centres de Recerca de Catalunya (CERCA) Program, Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya, Spanish Ministerio de Ciencia e Innovacion through the `Programa Estatal de Generacion de Conocimiento y Fortalecimiento Cientifico y Tecnologico del Sistema de I+D+i y del Programa Estatal de I+D+I Orientada a los Retos de la Sociedad', Proyectos de I+D+I Programacion Conjunta Internacional, Proyectos de I+D+I, European Social Fund, Instituto de Salud Carlos III through the program `Redes Tematicas de Investigacion Cooperativa en Salud (RETIC)', Junta de Andalucia, Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, Vanderbilt University School of Engineering, International Internship Program (IIIP), Institute for the International Education of Students, Spain, Lehigh University Iacocca International Internship Program (IIIP), and Institute

Subjects
RGD, Arginine-glycine-aspartic acid, Gastrulation, Nanopatterned substrates, Expression, Alpha-v-beta-3, Dynamics, Mesenchymal condensation, Mechanisms, Cell migration, Involvement, Chondrogenesis, Fibronectin, N-cadherin, Model
Abstract

Mesenchymal condensation is a prevalent morphogenetic transition that is essential in chondrogenesis. However, the current understanding of condensation mechanisms is limited. In vivo, progenitor cells directionally migrate from the surrounding loose mesenchyme towards regions of increasing matrix adherence (the condensation centers), which is accompanied by the upregulation of fibronectin. Here, we focused on the mechanisms of cell migration during mesenchymal cell condensation and the effects of matrix adherence. Dendrimer-based nanopatterns of the cell-adhesive peptide arginine-glycine-aspartic acid (RGD), which is present in fibronectin, were used to regulate substrate adhesion. We recorded collective and single-cell migration of mesenchymal stem cells, under chondrogenic induction, using live-cell imaging. Our results show that the cell migration mode of single cells depends on substrate adhesiveness, and that cell directionality controls cell condensation and the fusion of condensates. Inhibition experiments revealed that cell???cell interactions mediated by N-cadherin (also known as CDH2) are also pivotal for directional migration of cell condensates by maintaining cell???cell cohesion, thus suggesting a fine interplay between cell???matrix and cell???cell adhesions. Our results shed light on the role of cell interactions with a fibronectin-depositing matrix during chondrogenesis in vitro, with possible applications in regenerative medicine.

Published
2022

6. Antigenic nanostructures based on amoxicilloyl-bidendrons for effector cell activation to study allergic reactions to amoxicillin

Author

Ayane, Amene, Benedé, Sara, Morgado, Anjara, Vida, Yolanda, Rodríguez-Nogales, Alba, Fernández, Tahia, Paris, Juan L., Jiménez, Isabel, Bogas, Gador, Mayorga, Cristobalina, Pérez-Inestrosa, Ezequiel, Torres, María J., Montañez, M. I., Ayane, Amene, Benedé, Sara, Morgado, Anjara, Vida, Yolanda, Rodríguez-Nogales, Alba, Fernández, Tahia, Paris, Juan L., Jiménez, Isabel, Bogas, Gador, Mayorga, Cristobalina, Pérez-Inestrosa, Ezequiel, Torres, María J., and Montañez, M. I.

Published
2023

7. Antigenic Nanostructures based on Amoxicilloyl-bidendrons for Effector Cell Activation to Study Allergic Reactions to Amoxicillin

Author

Ayane, Amene, primary, Benedé, Sara, additional, Morgado, Anjara Anjara, additional, Vida, Yolanda, additional, Rodríguez-Nogales, Alba, additional, Fernández, Tahia T.D., additional, Paris, Juan J.L., additional, Jiménez, Isabel, additional, Bogas, Gador, additional, Mayorga, Cristobalina, additional, Pérez-Inestrosa, Ezequiel, additional, Torres, Maria, additional, and Montañez, Maria, additional

Published
2023
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12. New Insights into Acylhydrazones E / Z Isomerization: An Experimental and Theoretical Approach †.

Author

Fernández-Palacios, Sara, Matamoros, Esther, Morato Rojas, Isabel, López Navarrete, Juan T., Ruiz Delgado, M. Carmen, Vida, Yolanda, and Perez-Inestrosa, Ezequiel

Subjects
ISOMERIZATION, HYDROGEN bonding, INFORMATION storage & retrieval systems
Abstract

A family of acylhydrazones have been prepared and characterized with the aim of investigating their potential as information storage systems. Their well-established synthetic methodologies allowed for the preparation of seven chemically stable acylhydrazones in excellent yields that have been photophysically and photochemically characterized. In addition, DFT and TD-DFT calculations have been performed to gain more insights into the structural, energetic and photophysical properties of the E/Z isomers. Our results reveal that E/Z configurational isomerization upon irradiation is highly dependent on the stabilization of the E or Z isomers due to the formation of intramolecular H bonds and the electronic/steric effects intrinsically related to their structures. In addition, Raman spectroscopy is also used to confirm the molecular structural changes after the formation of hydrogen bonds in the isomers. [ABSTRACT FROM AUTHOR]

Published
2023
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13. Synthetic antigenic determinants of clavulanic acid induce dendritic cell maturation and specific T cell proliferation in patients with immediate hypersensitivity reactions

Author

Fernandez‐Santamaria, Ruben, primary, Bogas, Gador, additional, Montañez, Maria Isabel, additional, Ariza, Adriana, additional, Salas, Maria, additional, Cespedes, Jose Antonio, additional, Labella, Marina, additional, Paris, Juan Luis, additional, Perez‐Sanchez, Natalia, additional, Perez‐Inestrosa, Ezequiel, additional, Vida, Yolanda, additional, Fernandez, Tahia D., additional, Mayorga, Cristobalina, additional, and Torres, Maria Jose, additional

Published
2022
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16. Diseño de dendrímeros amino-terminales basados en enlaces tipo amidas. Estructuras con menor repulsión estérica y síntesis a gran escala

Author

Perez-Inestrosa, Ezequiel, Najera, Francisco, Morgado, Anjara, and Vida, Yolanda

Subjects
Biotecnología, Dendímeros, Dendrímeros, Biomedicina
Abstract

La estructura ramificada y la elevada multivalencia que presentan los dendrímeros los convierten en excelentes compuestos con potenciales aplicaciones en el campo de la biomedicina. En concreto, los dendrímeros con grupos amino terminales han resultado ser una herramienta muy útil en este campo gracias a la gran versatilidad que presentan. Nuestro grupo de investigación tiene una gran experiencia en el diseño y síntesis de dendrímeros amino-terminales [1]. La inserción de estas unidades dendriméricas en complejos luminiscentes nos ha permitido obtener macromoléculas con excelentes propiedades como biomarcadores [2]. Además, la modificación superficial de implantes orales de titanio con este tipo de estructuras ha contribuido a mejorar la biocompatibilidad de los mismos [3]. En esta comunicación presentamos una nueva familia de dendrímeros de poliamida amino-terminales. Su diseño implica una mayor separación entre los grupos terminales, generando así estructuras con menor congestión estérica. La metodología sintética se basa en procedimientos bien establecidos, utilizando reactivos sencillos y económicos, con rendimientos superiores al 90%, en escala de gramos y evitando procedimientos de purificación tediosos [4]

Published
2022

23. The Role of Benzylpenicilloyl Epimers in Specific IgE Recognition

Author

Mayorga, Cristobalina, primary, Montañez, Maria I., additional, Najera, Francisco, additional, Bogas, Gador, additional, Fernandez, Tahía D., additional, Gil, David Rodríguez, additional, Palacios, Ricardo, additional, Torres, Maria J., additional, Vida, Yolanda, additional, and Perez-Inestrosa, Ezequiel, additional

Published
2021
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24. Dynamic Covalent Properties of a Novel Indolo[3,2‐ b ]carbazole Diradical

Author

Badía‐Domínguez, Irene, primary, Peña‐Álvarez, Miriam, additional, Wang, Deliang, additional, Pérez Guardiola, Andrés, additional, Vida, Yolanda, additional, Rodríguez González, Sandra, additional, López Navarrete, Juan T., additional, Hernández Jolín, Víctor, additional, Sancho García, Juan C., additional, García Baonza, Valentín, additional, Nash, Rosie, additional, Hartl, František, additional, Li, Hongxiang, additional, and Ruiz Delgado, M. Carmen, additional

Published
2021
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26. The Janus Role of Adhesion in Chondrogenesis

Author

Casanellas, Ignasi, primary, Lagunas, Anna, additional, Vida, Yolanda, additional, Pérez-Inestrosa, Ezequiel, additional, Andrades, José A., additional, Becerra, José, additional, and Samitier, Josep, additional

Published
2020
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27. RGD-Dendrimer-Poly(L-lactic) Acid Nanopatterned Substrates for the Early Chondrogenesis of Human Mesenchymal Stromal Cells Derived from Osteoarthritic and Healthy Donors

Author

Rodríguez-Pereira, Cristina, primary, Lagunas, Anna, additional, Casanellas, Ignasi, additional, Vida, Yolanda, additional, Pérez-Inestrosa, Ezequiel, additional, Andrades, José A., additional, Becerra, José, additional, Samitier, Josep, additional, Blanco, Francisco J., additional, and Magalhães, Joana, additional

Published
2020
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29. MitoBlue as a tool to analyze the mitochondria-lysosome communication

Author

Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares, Universidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular, Universidade de Santiago de Compostela. Departamento de Química Orgánica, Sánchez López, Mateo Isidro, Vida, Yolanda, Pérez-Inestrosa, Ezequiel, Mascareñas Cid, José Luis, Vázquez Sentís, Marco Eugenio, Sugiura, Ayumu, Martínez Costas, José Manuel, Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares, Universidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular, Universidade de Santiago de Compostela. Departamento de Química Orgánica, Sánchez López, Mateo Isidro, Vida, Yolanda, Pérez-Inestrosa, Ezequiel, Mascareñas Cid, José Luis, Vázquez Sentís, Marco Eugenio, Sugiura, Ayumu, and Martínez Costas, José Manuel

Abstract

MitoBlue is a fluorescent bisamidine that can be used to easily monitor the changes in mitochondrial degradation processes in different cells and cellular conditions. MitoBlue staining pattern is exceptional among mitochondrial dyes and recombinant fluorescent probes, allowing the dynamic study of mitochondrial recycling in a variety of situations in living cells. MitoBlue is a unique tool for the study of these processes that will allow the detailed characterization of communication between mitochondria and lysosomes

Published
2020

30. RGD-dendrimer-poly(L-lactic) acid nanopatterned substrates for the early chondrogenesis of human mesenchymal stromal cells derived from osteoarthritic and healthy donors

Author

Rodríguez-Pereira, Cristina, Lagunas, Anna, Casanellas, Ignasi, Vida, Yolanda, Pérez-Inestrosa, Ezequiel, Andrades, José A., Becerra, José, Samitier, Josep, Blanco García, Francisco J, Magalhaes, Joana, Rodríguez-Pereira, Cristina, Lagunas, Anna, Casanellas, Ignasi, Vida, Yolanda, Pérez-Inestrosa, Ezequiel, Andrades, José A., Becerra, José, Samitier, Josep, Blanco García, Francisco J, and Magalhaes, Joana

Abstract

[Abstract] Aiming to address a stable chondrogenesis derived from mesenchymal stromal cells (MSCs) to be applied in cartilage repair strategies at the onset of osteoarthritis (OA), we analyzed the effect of arginine–glycine–aspartate (RGD) density on cell condensation that occurs during the initial phase of chondrogenesis. For this, we seeded MSC-derived from OA and healthy (H) donors in RGD-dendrimer-poly(L-lactic) acid (PLLA) nanopatterned substrates (RGD concentrations of 4 × 10−9, 10−8, 2.5 × 10−8, and 10−2 w/w), during three days and compared to a cell pellet conventional three-dimensional culture system. Molecular gene expression (collagens type-I and II–COL1A1 and COL2A1, tenascin-TNC, sex determining region Y-box9-SOX9, and gap junction protein alpha 1–GJA1) was determined as well as the cell aggregates and pellet size, collagen type-II and connexin 43 proteins synthesis. This study showed that RGD-tailored first generation dendrimer (RGD-Cys-D1) PLLA nanopatterned substrates supported the formation of pre-chondrogenic condensates from OA- and H-derived human bone marrow-MSCs with enhanced chondrogenesis regarding the cell pellet conventional system (presence of collagen type-II and connexin 43, both at the gene and protein level). A RGD-density dependent trend was observed for aggregates size, in concordance with previous studies. Moreover, the nanopatterns’ had a higher effect on OA-derived MSC morphology, leading to the formation of bigger and more compact aggregates with improved expression of early chondrogenic markers.

Published
2020

34. Dynamic Covalent Properties of a Novel Indolo[3,2‐b]carbazole Diradical.

Author

Badía‐Domínguez, Irene, Peña‐Álvarez, Miriam, Wang, Deliang, Pérez Guardiola, Andrés, Vida, Yolanda, Rodríguez González, Sandra, López Navarrete, Juan T., Hernández Jolín, Víctor, Sancho García, Juan C., García Baonza, Valentín, Nash, Rosie, Hartl, František, Li, Hongxiang, and Ruiz Delgado, M. Carmen

Subjects
HIGH temperatures, RADICAL anions, ELECTRON paramagnetic resonance spectroscopy, CARBAZOLE, HYDROSTATIC pressure, CYCLIC voltammetry
Abstract

This work describes the synthesis and properties of a dicyanomethylene‐substituted indolo[3,2‐b]carbazole diradical ICz‐CN. This quinoidal system dimerises almost completely to (ICz‐CN)2, which contains two long C(sp3)−C(sp3) σ‐bonds between the dicyanomethylene units. The minor open‐shell ICz‐CN component in the solid‐state mixture was identified by EPR spectroscopy. Cyclic voltammetry and UV–visible spectroelectrochemical data, as well as comparison with reference monomer ICz‐Br reveal that the nature of the one‐electron oxidation of (ICz‐CN)2 at ambient temperature and ICz‐CN at elevated temperature is very similar in all these compounds due to the prevailing localization of their HOMO on the ICz backbone. The peculiar cathodic behaviour reflects the co‐existence of (ICz‐CN)2 and ICz‐CN. The involvement of the dicyanomethylene groups stabilizes the close‐lying LUMO and LUMO+1 of (ICz‐CN)2 and especially ICz‐CN compared to ICz‐Br, resulting in a distinctive cathodic response at low overpotentials. Differently from neutral ICz‐CN, its radical anion and dianion are remarkably stable under ambient conditions. The UV/Vis(–NIR) electronic transitions in parent (ICz‐CN)2 and ICz‐CN and their different redox forms have been assigned convincingly with the aid of TD‐DFT calculations. The σ‐bond in neutral (ICz‐CN)2 is cleaved in solution and in the solid‐state upon soft external stimuli (temperature, pressure), showing a strong chromism from light yellow to blue–green. Notably, in the solid state, the monomeric diradical species is predominantly formed under high hydrostatic pressure (>1 GPa). [ABSTRACT FROM AUTHOR]

Published
2021
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36. Tuning emission properties of iridium and ruthenium metallosurfactants in micellar systems

Author

Guerrero-Martinez, Andres, Vida, Yolanda, Dominguez-Gutierrez, David, Albuquerque, Rodrigo Q., and De Cola, Luisa

Subjects
Iridium -- Chemical properties, Iridium -- Optical properties, Organometallic compounds -- Chemical properties, Organometallic compounds -- Optical properties, Ruthenium -- Optical properties, Ruthenium -- Chemical properties, Surface active agents -- Chemical properties, Surface active agents -- Optical properties, Chemistry
Published
2008

37. Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Author

Casanellas, Ignasi, primary, Lagunas, Anna, primary, Tsintzou, Iro, primary, Vida, Yolanda, primary, Collado, Daniel, primary, Pérez-Inestrosa, Ezequiel, primary, Rodríguez-Pereira, Cristina, primary, Magalhaes, Joana, primary, Gorostiza, Pau, primary, Andrades, José A., primary, Becerra, José, primary, and Samitier, Josep, primary

Published
2018
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41. 7th Drug hypersensitivity meeting: part two

Author

Elera, Javier Dionicio, primary, Boteanu, Cosmin, additional, Blanco, Maria Aranzazu Jimenez, additional, Gonzalez-Mendiola, Rosario, additional, García, Irene Carrasco, additional, Alvarez, Antonio, additional, Martinez, Jose Julio Laguna, additional, Garrido, Jaume Martí, additional, Barona, Carla Torán, additional, Chorda, Carolina Perales, additional, Salgueiro, Ramón López, additional, Palacios, Miguel Díaz, additional, De Rojas, Dolores Hernández Fernández, additional, Acar, Emre Ali, additional, Aktas, Ayse, additional, Ermertcan, Aylin Türel, additional, Temiz, Peyker, additional, Lin, Chien-Yio, additional, Hui, Chung-Yee Rosaline, additional, Chang, Ya-Ching, additional, Yang, Chih-Hsun, additional, Chung, Wen-Hung, additional, Carolino, Fabrícia, additional, Silva, Diana, additional, De Castro, Eunice Dias, additional, Cernadas, Josefina R., additional, Ensina, Luis Felipe, additional, Aranda, Carolina, additional, Nunes, Ines Camelo, additional, Lacerda, Alex, additional, Martins, Ana Maria, additional, Goudouris, Ekaterini, additional, Ribeiro, Marcia, additional, Da Silva Franco, José Francisco, additional, Queiroz, Leandra, additional, Solé, Dirceu, additional, Dalgiç, Ceyda Tunakan, additional, Sin, Aytül Zerrin, additional, Günsen, Fatma Düsünür, additional, Bulut, Gökten, additional, Ardeniz, Fatma Ömür, additional, Gülbahar, Okan, additional, Gökmen, Emine Nihal Mete, additional, Kokuludag, Ali, additional, De Francisco, Ana M. Montoro, additional, De Vicente Jiménez, Talía Mª, additional, Mendoza Parra, Adriana M., additional, Burgos Pimentel, Angella M., additional, Luque, Amelia García, additional, Amaral, Luis, additional, Leão, Leonor Carneiro, additional, Pinto, Nicole, additional, Belo, Joana, additional, Marques, João, additional, Carreiro-Martins, Pedro, additional, Leiria-Pinto, Paula, additional, Chaabane, Amel, additional, Romdhane, Haifa Ben, additional, Fredj, Nadia Ben, additional, Chadly, Zohra, additional, Boughattas, Naceur A., additional, Aouam, Karim, additional, Uyttebroek, Astrid P., additional, Bridts, Chris H., additional, Romano, Antonino, additional, Ebo, Didier G., additional, Sabato, Vito, additional, Lopes, Anabela, additional, Cosme, Joana, additional, Aguiar, Rita, additional, Lourenço, Tatiana, additional, Paes, Maria-João, additional, Spínola-Santos, Amélia, additional, Pereira-Barbosa, Manuel, additional, Cruz, Cíntia Rito, additional, Dos Reis, Rute Pereira, additional, Tomaz, Elza, additional, Pires, Ana Paula, additional, Inácio, Filipe, additional, Benito-Garcia, Filipe, additional, Mota, Inês, additional, Correia, Magna, additional, Gaspar, Ângela, additional, Chambel, Marta, additional, Piedade, Susana, additional, Morais-Almeida, Mário, additional, Nakonechna, Alla, additional, Antipkin, Yurij, additional, Umanets, Tetiana, additional, Pineda, Fernando, additional, Arribas, Francisca, additional, Lapshyn, Volodymyr, additional, Miranda, Pablo Andrés, additional, De La Cruz Hoyos, Bautista, additional, Blanco, Aranzazu Jimenez, additional, Del Pozo, Marta, additional, Vultaggio, Alessandra, additional, Nencini, Francesca, additional, Pratesi, Sara, additional, Matucci, Andrea, additional, Maggi, Enrico, additional, Cegec, Ivana, additional, Nahal, Danica Juricic, additional, Turk, Viktorija Erdeljic, additional, Aumiler, Matea Radacic, additional, Ausperger, Ksenija Makar, additional, Kraljickovic, Iva, additional, Simic, Iveta, additional, Yamaguchi, Yukie, additional, Watanabe, Tomoya, additional, Satoh, Megumi, additional, Tanegashima, Tomohiko, additional, Oda, Kayoko, additional, Wada, Hidefumi, additional, Aihara, Michiko, additional, Lee, Jaechun Jason, additional, Choi, Jay Chol, additional, Lee, Hwa Young, additional, Fernandes, Rosa-Anita Rodrigues, additional, Faria, Emília, additional, Pita, Joana, additional, Sousa, Nuno, additional, Ribeiro, Carmelita, additional, Carrapatoso, Isabel, additional, Bom, Ana Todo, additional, Rodolfo, Ana, additional, Dias-Castro, Eunice, additional, Voronova, Marina, additional, Valle, Diana Kury, additional, Coronel, Verónica Pacheco, additional, Chordá, Carolina Perales, additional, Madamba, Roselle Catherine Yu, additional, Ferrer, Marta, additional, Goikoetxea, Maria Jose, additional, D’Amelio, Carmen, additional, Bernad, Amalia, additional, Vega, Olga, additional, Gastaminza, Gabriel, additional, Bibián, Beatriz Pola, additional, Salazar, Marina Lluncor, additional, Vilà-Nadal, Gemma, additional, Roman, Ana María Fiandor, additional, Ortega, Javier Dominguez, additional, Muñoz, Miguel Gonzalez, additional, Gancedo, Santiago Quirce, additional, Moreno, Maria Rosario Cabañas, additional, Hofmeier, Kathrin Scherer, additional, Barzylovych, Vladyslava, additional, Pola, Beatriz, additional, Lluncor, Marina, additional, Fiandor, Ana, additional, Bellón, Teresa, additional, Domínguez, Javier, additional, Quirce, Santiago, additional, Yang, Min-Suk, additional, Kim, Sun-Sin, additional, Kim, Sae-Hoon, additional, Kang, Hye-Ryun, additional, Park, Heung-Woo, additional, Cho, Sang-Heon, additional, Min, Kyung-Up, additional, Chang, Yoon-Seok, additional, Delahaye, Clémence, additional, Flabbee, Jenny, additional, Waton, Julie, additional, Bauvin, Olivia, additional, Barbaud, Annick, additional, Fadhel, Najah Ben, additional, Gulin, Sandra Jerkovic, additional, Chiriac, Anca, additional, Cardoso, Bárbara Kong, additional, Viseu, Regina, additional, Moreira, Ana, additional, Cadinha, Susana, additional, Neves, Ana Castro, additional, Barreira, Patrícia, additional, Malheiro, Daniela, additional, Da Silva, J. P. Moreira, additional, Jurakic-Toncic, Ružica, additional, Ljubojevic, Suzana, additional, Turcic, Petra, additional, Gilissen, Liesbeth, additional, Huygens, Sara, additional, Goossens, An, additional, Andreu, Inmaculada, additional, Romero, Alicia Martinez, additional, Cabezas, Pau Gomez, additional, Parejo, Pedro Ayuso, additional, Del Carmen Plaza-Serón, Maria, additional, Doña, Inmaculada, additional, Blanca-López, Natalia, additional, Flores, Carlos, additional, Galindo, María Luisa, additional, Molina, Ana, additional, Perkins, James Richard, additional, Cornejo-García, José Antonio, additional, García-Agúndez, José Augusto, additional, García-Martín, Elena, additional, Campo, Paloma, additional, Canto, María Gabriela, additional, Blanca, Miguel, additional, Guéant-Rodríguez, Rosa María, additional, Jurado-Escobar, Raquel, additional, Barrionuevo, Esther, additional, Salas, María, additional, Canto, Gabriela, additional, Guéant, Jean-Louis, additional, Usui, Toru, additional, Tailor, Arun, additional, Faulkner, Lee, additional, Farrell, John, additional, Alfirevic, Ana, additional, Kevin Park, B., additional, Naisbitt, Dean J., additional, Trelles, Oswaldo, additional, Guerrero, María Auxiliadora, additional, Upton, Alex, additional, Ueta, Mayumi, additional, Sawai, Hiromi, additional, Sotozono, Chie, additional, Tokunaga, Katushi, additional, Kinoshita, Shigeru, additional, Sukasem, Chonlaphat, additional, Satapornpong, Patompong, additional, Tempark, Therdpong, additional, Rerknimitr, Pawinee, additional, Pairayayutakul, Kulprapat, additional, Klaewsongkram, Jettanong, additional, Koomdee, N., additional, Jantararoungtong, T., additional, Santon, S., additional, Puangpetch, A., additional, Intusoma, U., additional, Tassaneeyakul, W., additional, Theeramoke, V., additional, Ramirez, Elena, additional, Borobia, Alberto Manuel, additional, Tong, Hoi, additional, Castañer, Jose Luis, additional, De Abajo, Francisco José, additional, Galvao, Violeta Régnier, 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Published
2016
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44. Study of protein haptenation by amoxicillin through the use of a biotinylated antibiotic

Author

Ariza, Adriana, Collado, Daniel, Vida, Yolanda, Montañez, M. I., Pérez-Inestrosa, Ezequiel, Blanca, Miguel, Torres, María J., Cañada, F. Javier, Pérez-Sala, Dolores, Ariza, Adriana, Collado, Daniel, Vida, Yolanda, Montañez, M. I., Pérez-Inestrosa, Ezequiel, Blanca, Miguel, Torres, María J., Cañada, F. Javier, and Pérez-Sala, Dolores

Abstract

Allergic reactions towards b-lactam antibiotics pose an important clinical problem. The ability of small molecules, such as a b-lactams, to bind covalently to proteins, in a process known as haptenation, is considered necessary for induction of a specific immunological response. Identification of the proteins modified by b-lactams and elucidation of the relevance of this process in allergic reactions requires sensitive tools. Here we describe the preparation and characterization of a biotinylated amoxicillin analog (AX-B) as a tool for the study of protein haptenation by amoxicillin (AX). AX-B, obtained by the inclusion of a biotin moiety at the lateral chain of AX, showed a chemical reactivity identical to AX. Covalent modification of proteins by AX-B was reduced by excess AX and vice versa, suggesting competition for binding to the same targets. From an immunological point of view, AX and AX-B behaved similarly in RAST inhibition studies with sera of patients with non-selective allergy towards b-lactams, whereas, as expected, competition by AX-B was poorer with sera of AX-selective patients, which recognize AX lateral chain. Use of AX-B followed by biotin detection allowed the observation of human serum albumin (HSA) modification by concentrations 100-fold lower that when using AX followed by immunological detection. Incubation of human serum with AX-B led to the haptenation of all of the previously identified major AX targets.In addition, some new targets could be detected. Interestingly, AX-B allowed the detection of intracellular protein adducts, which showed a cell type-specific pattern. This opens the possibility of following the formation and fate of AX-B adducts in cells. Thus, AX-B may constitute a valuable tool for the identification of AX targets with high sensitivity as well as for the elucidation of the mechanisms involved in allergy towards b-lactams.

Published
2014

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