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The transition of females from childbirth to lactation is a physiologically complex period characterised by metabolic, endocrine and immunological changes. In addition, difficult labour drains the female and can lead to hypolactia. The purpose of the study was to determine the effect of probiotics on lactation recovery and metabolic changes in the body of females. Among the methods used are: the determination of lactation level, the physiological method for determining live weight, the biochemical method for blood testing; and the statistical method. The application of Bacillus subtilis AX 20, Bacillus licheniformis EA 22 contributes to an increase in milk productivity in cows on day 7-9 of the study by 12.9%, on day 10-12 – by 15.03%, on day 13-15 – by 13.93%, on day 16-18 – by 13.5% and on day 19-21 – by 21.35% compared to the control. The total protein content in experimental cows increased by 18.92%, globulins – by 37.26 (p≤0.05), compared to the initial indicators. The activity of alanine aminotransferase, urea, and urea nitrogen in animals of the control and experimental groups was within the normal range during the experiment. The application of Bacillus megaterium NCH 55 to sows with hypolactation contributes to lactation recovery in sows. In the experimental group, piglets showed a lag in growth of 18.5% on the third day and 11.39% on the tenth day due to hypolactation. Starting from day 15, there was an increase in the live weight of suckling piglets due to the restoration of lactation in sows by 20.56%, on day 20 – by 20.43%, on day 25 – by 30.56%, on day 30 – by 31.91%, compared to the control. In the blood serum of experimental sows, the content of total protein increased by 10%, globulins – by 19.13%, total cholesterol – by 40.11%, urea by 48.0%, compared to the beginning of studies (p≤0.05). The activity of alanine aminotransferase in the experiment increased by 8.95% (p≤0.05), alkaline phosphatase decreased by 27.46% (p≤0.05), compared to the beginning of the study. In the blood of experimental sows, the level of circulating immune complexes increased by 42.85% and a decrease in seromucoids by 30.43%. The practical value of the study lies in the use of probiotics to restore the milk productivity of cows and sows after childbirth