227 results on '"Vico, Ivana"'
Search Results
2. Specific detection of Waiteacircinata var.zeae using conventional and real-time PCR
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Vojvodić, Mira, Lazić, Dejan, Pešić, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
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- 2024
- Full Text
- View/download PDF
3. Specific detection of Waitea circinata var. zeae using conventional and real-time PCR
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Vojvodić, Mira, primary, Lazić, Dejan, additional, Pešić, Brankica, additional, Mitrović, Petar, additional, Vico, Ivana, additional, and Bulajić, Aleksandra, additional
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- 2024
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4. The dual nature of Lambertella corni-maris as an apple fruit pathogen and antagonist of Monilinia spp
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Vasić, Miljan, Vico, Ivana, Jurick, II, Wayne M., Duduk, Bojan, and Duduk, Nataša
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- 2022
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5. First report of Botryosphaeria dothidea causing postharvest rot of quince fruits in Serbia
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Vučković, Nina, Vico, Ivana, and Duduk, Nataša
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- 2023
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6. Phytoplasmas infecting sugar beet in central Europe
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Duduk, Bojan, Duduk, Bojan, Kosovac, Andrea, Stepanović, Jelena, Rekanović, Emil, Ćurčić, Živko, Vučković, Nina, Duduk, Nataša, Vico, Ivana, Duduk, Bojan, Duduk, Bojan, Kosovac, Andrea, Stepanović, Jelena, Rekanović, Emil, Ćurčić, Živko, Vučković, Nina, Duduk, Nataša, and Vico, Ivana
- Abstract
In Europe, the cultivation of sugar beet faces significant challenges due to the emergence of basses richesses syndrome (SBR) and rubbery taproot disease (RTD) which are associated with fastidious bacteria Candidatus Arsenophonus phytopathogenicus and Candidatus Phytoplasma solani, respectively. A comprehensive study conducted from 2018 to 2022 aimed to elucidate the prevalence and distribution of these pathogens in sugar beet in central Europe, focusing on the Pannonian Plain (particularly Serbia and Slovakia where RTD has been present in epidemic scales) and Germany.
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- 2024
7. Specific detection of Waitea circinata var. zeae using conventional and real-time PCR
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Vojvodić, Mira, Vojvodić, Mira, Lazić, Dejan, Pešić, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, Vojvodić, Mira, Vojvodić, Mira, Lazić, Dejan, Pešić, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
- Abstract
Waitea circinata var. zeae, a pathogen with a relatively narrow host range, has recently been detected in cabbage and oilseed rape in Europe and worldwide. In this study, we developed specific conventional and real-time PCR protocols for direct detection of W. circinata var. zeae from mycelium and diseased plant tissue. The newly developed primer pair zeaefor1/zeaerew1, used in PCR protocols, specifically amplified only target isolates of W. circinata var. zeae when tested against isolates of 11 different binucleate and multinucleate anastomosis groups of Rhizoctonia spp. including AG-A, AG-G, AG-F, AG-U, AG-2-1, AG-2-2, AG-3, AG-4 HGI, AG-4 HGII, AG-4 HGIII, and AG-6 and common soil-borne pathogens. Total of nine previously published primer pairs designed for the detection of various Rhizoctonia spp. were also tested and did not amplify target isolates of W. circinata var. zeae. The detection limit of conventional and real-time PCR protocols was 10–2 and 10–5 (with starting concentration 9.5 ng/µl), respectively, and both methods are the first available tools for direct detection and identification of W. circinata var. zeae from mycelium and diseased oilseed rape seedlings. Both conventional and SYBR-Green-based real-time PCR protocols are cost-effective and provide a solid basis for further investigations of W. circinata var. zeae, particularly in relation to distribution, host range, and epidemiology.
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- 2024
8. Geographical variations, prevalence, and molecular dynamics of fastidious phloem-limited pathogens infecting sugar beet across Central Europe.
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Duduk, Bojan, Stepanović, Jelena, Fránová, Jana, Zwolińska, Agnieszka, Rekanović, Emil, Stepanović, Miloš, Vučković, Nina, Duduk, Nataša, and Vico, Ivana
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MOLECULAR dynamics ,ROOT rots ,MACROPHOMINA phaseolina ,PATHOGENIC microorganisms ,DISEASE outbreaks ,PHYTOPLASMAS - Abstract
In Europe, two fastidious phloem-limited pathogens, 'Candidatus Phytoplasma solani' (16SrXII-A) and 'Candidatus Arsenophonus phytopathogenicus', are associated with rubbery taproot disease (RTD) and syndrome basses richesses (SBR) of sugar beet, respectively. Both diseases can significantly reduce yield, especially when accompanied by root rot fungi. This study investigates the presence, geographic distribution and genetic traits of fastidious pathogens and the accompanying fungus, Macrophomina phaseolina, found on sugar beet across four geographically separated plains spanning seven countries in Central Europe. The survey revealed variable incidences of symptoms linked to these fastidious pathogens in the Pannonian and Wallachian Plains, sporadic occurrence in the North European Plain, and no symptomatic sugar beet in the Bohemian Plain. Molecular analyses unveiled the occurrence of both 'Ca. P. solani' and 'Ca. A. phytopathogenicus' throughout Central Europe, with a predominance of the phytoplasma. These fastidious pathogens were detected in all six countries surveyed within the Pannonian and Wallachian Plains, with only a limited presence of various phytoplasmas was found in the North European Plain, while no fastidious pathogens were detected in Bohemia, aligning with observed symptoms. While 16S rDNA sequences of 'Ca. P. solani' remained highly conserved, multi-locus characterization of two more variable loci (tuf and stamp) unveiled distinct variability patterns across the plains. Notably, the surprising lack of variability of tuf and stamp loci within Central Europe, particularly the Pannonian Plain, contrasted their high variability in Eastern and Western Europe, corresponding to epidemic and sporadic occurrence, respectively. The current study provides valuable insights into the genetic dynamics of 'Ca. P. solani' in Central Europe, and novel findings of the presence of 'Ca. A. phytopathogenicus' in five countries (Slovakia, Czech Republic, Austria, Serbia, and Romania) and M. phaseolina in sugar beet in Slovakia. These findings emphasize the need for further investigation of vector–pathogen(s)–plant host interactions and ecological drivers of disease outbreaks. [ABSTRACT FROM AUTHOR]
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- 2024
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9. The possibility of coriander seed disinfection with the essential oil of peppermint
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Mikić Sara D., Vico Ivana M., Duduk Nataša D., and Marković Tatjana Lj.
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seed health ,coriander seed ,peppermint (mentha x piperita) essential oil ,alternaria sp. ,Agriculture - Abstract
Seed health testing aims to ensure a healthy crop and to prevent the spread of plant diseases. The need to study and apply both essential oils and plant extracts against seed diseases is growing and becoming more important. Coriander (Coriandrum sativum L.) is an annual herbaceous, aromatic, spicy and medicinal plant from the Apiaceae family, known for its seeds with a characteristic and pleasant odour. In human medicine, coriander is a part of various medicines for the treatment of digestive organs. Phytopathogenic fungi and bacteria are the most common and significant causes of coriander seed disease. The aim of this study was to assess coriander seed health and investigate the possibility of seed disinfection using peppermint (Mentha x piperita) essential oil. The health of the seeds was examined by the filter paper laboratory standard method. Different concentrations (1, 2, 5 and 10%) of peppermint essential oil were used for seed disinfection. The composition of the essential oil was determined by gas chromatographic analysis. The results of seed health analysis showed the presence of a fungus after seven days of incubation in a humid chamber at room temperature. The fungus was identified as Alternaria sp. (sect. Alternaria) based on the symptoms and macroscopic and microscopic features of the fungus (on seed and potato dextrose agar). Immersion treatment of infected seed in peppermint essential oil reduced the presence of Alternaria sp. on coriander seeds. The highest disease inhibition, 69,33%, namely 58,93% without the influence of the emulsifier, was shown by oil solution of 5%. Stagnation in inhibition was observed with a further increase in oil concentration. The results of this study show the possibility of disinfecting coriander seeds with peppermint essential oil to reduce infection with Alternaria sp.
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- 2021
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10. Antifungal and synergistic activity of five plant essential oils from Serbia against Trichoderma aggressivum f. europaeum Samuels & W. Gams
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Luković Jelena, Đurović-Pejčev Rada, Đorđević Tijana, Milijašević-Marčić Svetlana, Duduk Nataša, Vico Ivana, and Potočnik Ivana
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green mould disease ,button mushroom ,biofungicides ,essential oils ,Plant culture ,SB1-1110 - Abstract
Five essential oils isolated from plants originating from Serbia and ten combinations of the selected essential oils were assayed to test their inhibitory and fungicidal activity against Trchoderma aggressivum f. europaeum Samuels & W. Gams using two distinctive methods: microdilution and fumigant macrodilution methods. The strongest activity was demonstrated by spearmint (Mentha spicata L.) and thyme (Thymus serpyllum L.) oils at the minimum inhibitory concentration (MIC) of 6.25 µl ml-1 using microdilution, and 0.16 µl ml-1 of air using fumigant macrodilution method. The antifungal activity of basil (Ocimum basilicum L.) and peppermint (Mentha piperita L.) was medium, while the oil extracted from St. John's wort (Hypericum perforatum L.) exhibited the lowest activity. None of the selected essential oils exhibited fungicidal effect at minimal fungicidal concentrations (MFC) of over 25 µl ml-1 or 0.32 µl ml-1of air, using microand macrodilution, respectively. When microdilution was used, the strongest antifungal activity was demonstrated by two oil combinations: spearmint-thyme and spearmint-peppermint, having MIC and MFC values of 3.75 µl ml-1. The lowest activity was demonstrated by the basil-St. John's wort essential oil combination, at 30 µl ml-1 MIC, and MFC exceeding 30 µl ml-1. The obtained results indicate possible synergistic effects of essential oils and their components.
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- 2020
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11. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
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Vojvodić Mira, Lazić Dejan, Mitrović Petar, Tanović Brankica, Vico Ivana, and Bulajić Aleksandra
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Rhizoctonia solani AG-2-2 ,sugar beet ,morphology ,molecular identification ,specific detection, real-time PCR ,Plant culture ,SB1-1110 - Abstract
Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples.
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- 2019
12. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
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Vojvodić Mira, Tanović Brankica, Mihajlović Milica, Mitrović Petar, Vico Ivana, and Bulajić Aleksandra
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Rhizoctonia ,Hyphal anastomosis ,Binucleate AG-A ,ITS rDNA sequence analyses ,Pathogenicity ,Strawberry ,Plant culture ,SB1-1110 - Abstract
Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet.
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- 2018
13. Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk
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Luković Jelena, Stepanović Miloš, Todorović Biljana, Milijašević-Marčić Svetlana, Duduk Nataša, Vico Ivana, and Potočnik Ivana
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Button mushroom ,Essential oils ,Cinnamon ,Clove ,Fungal pathogens ,Antifungal activity ,Plant culture ,SB1-1110 - Abstract
Antifungal activity of two essential oils, cinnamon (Cinnamomum verum J. Presl) and clove (Syzygium aromaticum (L.) Merrill & Perry), was evaluated against Cladobotryum dendroides (Bull.) W. Gams & Hooz, and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, the causal agents of cobweb and dry bubble disease of cultivated mushroom. Inhibitory and fungicidal activity of the selected essential oils was assayed using three methods: microdilution, macrodilution fumigant and macrodilution contact method. Comparing all three methods, clove essential oil showed stronger activity than cinnamon against both fungi, having minimum inhibitory concentration (MIC) at the lowest concentrations tested (1.56, 0.02 and 0.1 µl ml-1, respectively). However, cinnamon oil was more toxic to L. fungicola var. fungicola then to C. dendroides in all three methods. Both oils exhibited stronger antifungal effects when used in the macrodilution fumigant than in contact method. The results showed that both cinnamon and clove essential oils have the potential for further in vivo experiments against L. fungicola var. fungicola and C. dendroides and indicated a possible use of these oils in integrated disease management in mushrooms.
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- 2018
14. Suitability of different primers for specific molecular detection of Monilinia spp.
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Duduk Nataša D., Vasić Miljan M., Vučković Nina R., Žebeljan Aleksandra A., and Vico Ivana M.
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Monilinia fructigena ,M. polystroma ,M. fructicola ,M. laxa ,PCR ,Multiplex PCR ,molecular identification ,Agriculture - Abstract
Monilinia spp. are economically important pathogens of pome and stone fruits. Four Monilinia species are present in Serbia - Monilinia fructigena, M. laxa, M. fructicola and Monilia polystroma. As detection and identification of Monilinia species are complex, the aim of this research was to evaluate species-specific primers in PCR in order to standardize fast and reliable molecular methods for differentiation between the four Monilinia species. Isolates of M. fructigena, M. laxa, M. fructicola and M. polystroma from apple fruit and referent isolates from Italy and Japan were used for testing. Specific molecular detection of M. laxa was obtained using ITS1Mlx/ITS4Mlx and Ml-Mfg-F2/Ml-Mfc-R1 primer pairs, and M. fructicola using ITS1Mfcl/ITS4Mfcl and Mfc-F1/Mfc-R1 primer pairs. ITS1Mfgn/ITS4Mfgn and ITS1/Mfg-R2 primer pairs, described as M. fructigena species-specific, amplified M. fructigena and M. polystroma, as well. Specific detection of these two species as well as of all four tested Monilinia species was obtained using the reverse primer MO368-5 with forward primers MO368-8R, Laxa-R2 and MO368-10R in separate or in Multiplex PCR reactions. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46008]
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- 2017
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15. A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot
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Duduk, Nataša, primary, Vico, Ivana, additional, Kosovac, Andrea, additional, Stepanović, Jelena, additional, Ćurčić, Živko, additional, Vučković, Nina, additional, Rekanović, Emil, additional, and Duduk, Bojan, additional
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- 2023
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16. Waitea circinata var. zeae – prouzrokovač truleži korena kupusa i uljane repice
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Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
- Abstract
Waitea circinata var. zeae (anamorf Rhizoctonia zeae) je značajan patogen uglavnom onokotiledonih biljaka, koji je prisutan u mnogim delovima sveta, a održava se u zemljištu duži niz godina. Poslednjih godina krug domaćina W. circinata var. zeae širi se i na neke dikotiledone biljke. U okviru nedavnih istraživanja u Srbiji W. circinata var. zeae je utvrđen kao prouzrokovač truleži korena i korenovog vrata, i propadanja biljaka kupusa i uljane repice na lokalitetima Futog i Rimski Šančevi. U radu su sistematizovani publikovani podaci o rasprostranjenosti, krugu domaćina, načinu održavanja i širenja, kao i suzbijanju W. circinata var. zeae, kao i rezultati istraživanja koja su obavljena u Srbiji.
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- 2023
17. A biotroph sets the stage for a necrotroph to play: ‘Candidatus Phytoplasma solani’ infection of sugar beet facilitated Macrophomina phaseolina root rot
- Author
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Duduk, Nataša, Duduk, Nataša, Vico, Ivana, Kosovac, Andrea, Stepanović, Jelena, Ćurčić, Živko, Vučković, Nina, Rekanović, Emil, Duduk, Bojan, Duduk, Nataša, Duduk, Nataša, Vico, Ivana, Kosovac, Andrea, Stepanović, Jelena, Ćurčić, Živko, Vučković, Nina, Rekanović, Emil, and Duduk, Bojan
- Abstract
‘Candidatus Phytoplasma solani’ (stolbur phytoplasma) is associated with rubbery taproot disease (RTD) of sugar beet (Beta vulgaris L.), while Macrophomina phaseolina is considered the most important root rot pathogen of this plant in Serbia. The high prevalence of M. phaseolina root rot reported on sugar beet in Serbia, unmatched elsewhere in the world, coupled with the notorious tendency of RTD-affected sugar beet to rot, has prompted research into the relationship between the two diseases. This study investigates the correlation between the occurrence of sugar beet RTD and the presence of root rot fungal pathogens in a semi-field ‘Ca. P. solani’ transmission experiment with the cixiid vector Reptalus quinquecostatus (Dufour), in addition to naturally infected sugar beet in the open field. Our results showed that: (i) Reptalus quinquecostatus transmitted ‘Ca. P. solani’ to sugar beet which induced typical RTD root symptoms; (ii) Macrophomina phaseolina root rot was exclusively present in ‘Ca. P. solani’-infected sugar beet in both the semi-field experiment and naturally infected sugar beet; and that (iii) even under environmental conditions favorable to the pathogen, M. phaseolina did not infect sugar beet, unless the plants had been previously infected with phytoplasma. Copyright © 2023 Duduk, Vico, Kosovac, Stepanović, Ćurčić, Vučković, Rekanović and Duduk.
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- 2023
18. Specific detection of Waiteacircinatavar.zeaeusing conventional and real-time PCR
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Vojvodić, Mira, Lazić, Dejan, Pešić, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
- Abstract
Waiteacircinatavar. zeae,a pathogen with a relatively narrow host range, has recently been detected in cabbage and oilseed rape in Europe and worldwide. In this study, we developed specific conventional and real-time PCR protocols for direct detection of W.circinatavar. zeaefrom mycelium and diseased plant tissue. The newly developed primer pair zeaefor1/zeaerew1, used in PCR protocols, specifically amplified only target isolates of W.circinatavar. zeaewhen tested against isolates of 11 different binucleate and multinucleate anastomosis groups of Rhizoctoniaspp. including AG-A, AG-G, AG-F, AG-U, AG-2-1, AG-2-2, AG-3, AG-4 HGI, AG-4 HGII, AG-4 HGIII, and AG-6 and common soil-borne pathogens. Total of nine previously published primer pairs designed for the detection of various Rhizoctoniaspp. were also tested and did not amplify target isolates of W.circinatavar. zeae. The detection limit of conventional and real-time PCR protocols was 10–2and 10–5(with starting concentration 9.5 ng/µl), respectively, and both methods are the first available tools for direct detection and identification of W.circinatavar. zeaefrom mycelium and diseased oilseed rape seedlings. Both conventional and SYBR-Green-based real-time PCR protocols are cost-effective and provide a solid basis for further investigations of W.circinatavar. zeae, particularly in relation to distribution, host range, and epidemiology.
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- 2024
- Full Text
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19. Waitea circinata var. zeae: Causing root rot of cabbage and oilseed rape
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Vojvodić, Mira, primary, Tanović, Brankica, additional, Mitrović, Petar, additional, Vico, Ivana, additional, and Bulajić, Aleksandra, additional
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- 2023
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20. Penicillium solitum produces a polygalacturonase isozyme in decayed Anjou pear fruit capable of macerating host tissue in vitro
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Jurick, Wayne M., Vico, Ivana, Gaskins, Verneta L., Whitaker, Bruce D., Garrett, Wesley M., Janisiewicz, Wojciech J., and Conway, William S.
- Published
- 2012
21. Pathogenic potential of Waitea circinata var. zeae
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Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
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soil-borne ,Waitea circinata var. zeae ,oilseed rape ,fungi ,detection ,food and beverages ,host range ,pathogens ,cabbage - Abstract
Waitea circinata Warcup & P.H.B. Talbot (multinuclear Rhizoctonia spp.) is a widespread, soil-borne plant pathogen causing root rot mainly of monocotyledonous plants. Among five described varieties, the most common and widespread is W. circinata var. zeae Toda, Mushika, T. Hayak., Tanaka, Tani & Hyakum. Besides monocotyledonous hosts, a natural infection of a limited number of dicotyledonous plants, including soybeans, beans, peas, sugar beets and carrots were recorded recently. During 2017, cabbage and oilseed rape plants showing symptoms of root and root neck rot, sampled at the localities of Futog and Rimski Šančevi (Serbia), were proved to be infected with W. circinata var. zeae. This was the first record of natural infection of plants from Brassicaceae family worldwide. Two selected isolates from cabbage (299-17) and oilseed rape (300-17) formed fast-growing orange colonies with red sclerotia and multinucleate hyphae with a characteristic branching pattern. The identification was further confirmed by BLAST analysis of sequences of ITS rDNA, RPB2, LSU and β-tubulin genomic regions. Koch’s postulates were fulfilled after inoculation of cabbage and oilseed rape seedlings, while an experimental host range was determined by inoculations of 14 different plant species from 8 families.
- Published
- 2022
22. Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia
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Žebeljan, Aleksandra, primary, Duduk, Nataša, additional, Vučković, Nina, additional, Jurick, Wayne M., additional, and Vico, Ivana, additional
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- 2021
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23. Multinucleate Rhizoctonia sp.: Pathogen of sugar beet and susceptibility of cultivars under field conditions
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Vico Ivana M., Janković Dragica B., Krstić Branka B., Bulajić Aleksandra R., and Dukić Nataša D.
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multinucleate Rhizoctonia sp. ,natural infection ,tolerant cultivars ,yield ,Science (General) ,Q1-390 - Abstract
Sugar beet root rot has severely occurred in our country recently, especially in localities of Pazova, Pećinci, Ruma, Sremska Mitrovica and Šid. From diseased roots as well as from soil collected from the localities where decay occurred, fungal isolates were obtained by bait plant method. Based on their characteristics, they were identified as multinucleate Rhizoctonia sp. During the year of 2004 in Mitrosrem trial field T-11, where the presence of multinucleate Rhizoctonia sp. was confirmed, an experiment under the coordination of Committee for Acknowledgement and Registration of New Cultivars in our country was conducted in order to determine cultivars' tolerance, i.e. their susceptibility and possibility for growing on infested fields. Six cultivars of sugar beet, Laetitia (as standard) and five new ones were included in the investigation. The trial was conducted in accordance with the established and accepted method (Ministry of Agriculture, Forestry and Water Resources, Republic of Serbia). Susceptibility of investigated cultivars was evaluated according to significant production characteristics root yield, sugar content, corrected sugar content, thick juice Q, molasses sugar, content of K, Na and amino-N, polarized sugar yield and white sugar yield, as it was recommended by the method. Conducted investigations have revealed that tested sugar beet cultivars showed different reactions to natural infection with multinucleate Rhizoctonia sp. Concerning root yield as the most important agricultural characteristic, statistically significantly higher yield was obtained with the cultivar under code mark 5 (61.120 kg/ha) whereas the cultivar marked under code 6 had significantly lower yield comparing to the standard (38.100 kg/ha).
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- 2006
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24. Diversity of Botryosphaeriaceae and Diaporthe species associated with postharvest apple fruit decay in Serbia
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Vučković, Nina, primary, Vico, Ivana, additional, Duduk, Bojan, additional, and Duduk, Natasa, additional
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- 2021
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25. Identification of viruses infecting pumpkins (Cucurbita pepo L) in Yugoslavia
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Krstić Branka B., Berenji Janoš B., Dukić Nataša D., Vico Ivana M., Katis Nikolaos I., and Papavassiliou Chryssa C.
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Cucurbita pepo ,pumpkins ,plant viruses ,zucchini yellow mosaic potyvirus ,cucumber mosaic cucumovirus ,watermelon mosaic potyvirus 1 ,watermelon mosaic potyvirus 2 ,biotest ,ELISA ,EBIA ,Science (General) ,Q1-390 - Abstract
This study was carried out in order to identify the major viruses infecting pumpkins (Cucurbita pepo)grown in Serbia. Leaf samples from virus-infected pumpkin plants were collected in mid-July 2001. Naked-seeded and hulled oil pumpkins, patty pan, zucchini and summer squash from three different locations were included (Table 1). Virus-infected plants showed different symptoms (Table 2 and Figures 1-4). Due to the great variability of the symptoms, the causal viruses could not be fully and precisely determined by visual examination only. The infected samples were tested by the biotest, as well as by two serological methods, ELISA and EBIA. Polyclonal antibodies raised against cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 1 (WMV-1), watermelon mosaic potyvirus 2 (WMV-2) and squash mosaic comovirus (SqMV) were used. In each of the 50 collected samples one or two viruses were detected (Tables 3 and 4). The most prevalent viruses infecting pumpkins were ZYMV (62%) and CMV (58%). WMV-2 was extremely rare.
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- 2002
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26. Differentiation of Rhizoctonia spp. Based on their antigenic properties
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Vico Ivana M., Krstić Branka B., and Dukić Nataša
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Rhizoctonia spp. ,binucleate Rhizoctonia ,Rhizoctonia solani ,anastomosis groups ,serological identification ,serological relationship ,dot-immunobinding assay ,Agriculture - Abstract
Antigenic properties and serological relationship was investigated in binucleate and multinucleate Rhizoctonia spp. isolates from strawberries soybean, alfalfa and potato plants from Serbia, from Spain, anastomosis group testers and in strawberry roots inoculated with binucleate Rhizoctonia AG A and AG I. Two polyclonal antisera, unabsorbed and cross absorbed, were used in dot-immunobinding assay for these investigations. Antisera were produced against mycelial antigens of two isolates, which belong to different anastomosis groups (AG) of binucleate Rhizoctonia - AG A and AG I. Both unabsorbed antisera reacted positively with all tested Rhizoctonia spp. isolates, and the reaction was absent with control isolates (Pythium sp. Agaricus sp. and Fusarium sp). The results prove a close serological relationship among Rhizoctonia spp. isolates, and diversity between Rhizoctonia spp. and isolates from different taxonomic groups. Also, both unabsorbed antisera reacted with higher intensity with closely related antigens (belonging to the same AG) than with ones from another AG of binucleate Rhizoctonia or R. solani (multinucleate Rhizoctonia). After cross absorption specificity of the antisera was enhanced, especially with the antiserum raised against mycelial proteins of binucleate Rhizoctonia AG I. This antiserum reacted positively only with antigens from the same AG, after cross absorption with antigens from AG A of binucleate Rhizoctonia and from R. solani AG 2-2. It proved to be specific to AG I of binucleate Rhizoctonia, and able to differentiate isolates of this AG from others. In this way the serological homology among isolates of one AG was proven, and also the diversity among isolates which belong to different AGs of binucleate Rhizoctonia as well as isolates of R. solani.
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- 2002
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27. Biological and serological characterization of viruses of summer squash crops in Yugoslavia
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Dukić Nataša M., Krstić Branka B., Vico Ivana M., Katis N.I., Papavassiliou Chryssa, and Berenji Janoš B.
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summer squash crop ,plant viruses ,zucchini yellow mosaic potyvirus ,watermelon mosaic potyvirus 2 ,cucumber mosaic cucumovirus ,bioassay ,serology ,Agriculture - Abstract
A survey on summer squash open field crops was carried out during 2000 and 2001 in order to identify the major viruses infecting these crops in different localities. Plants showed different types of symptoms: mild mosaic, chlorotic spotting, distinctive mosaic, blistering of leaf lamina leaf yellowing, deformation of leaf lamina, knobbed fruits and stunting of plants. The symptoms were very variable but showed the viral nature of the investigated summer squash diseases. The collected samples were tested by bioassay and by two serological methods ELISA and EBIA using cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2), zucchini yellow flack potyvirus (ZYFV) watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) and cucurbit aphid-borne yellows polerovirus (CABYV) polyclonal antisera. In all tested samples single or mixed infection with ZYMV, CMV and WMV-2 was detected. The most prevalent virus infecting summer squash was ZYMV. This is the first report of ZYMV, the most destructive virus infecting cucurbits, in Yugoslavia. It was also proven that the identified viruses are transmissible by Aphis gossypii in a non-persistent manner, but possible role of seed in virus transmission was not confirmed.
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- 2002
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28. The possibility of coriander seed disinfection with the essential oil of peppermint [Mogućnost dezinfekcije semena korijandera etarskim uljem pitome nane]
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Mikić, Sara D., Vico, Ivana M., Duduk, Nataša D., and Marković, Tatjana Lj.
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Alternaria sp ,Peppermint (Mentha x piperita) essential oil ,Seed health ,Coriander seed ,food and beverages - Abstract
Seed health testing aims to ensure a healthy crop and to prevent the spread of plant diseases. The need to study and apply both essential oils and plant extracts against seed diseases is growing and becoming more important. Coriander (Coriandrum sativum L.) is an annual herbaceous, aromatic, spicy and medicinal plant from the Apiaceae family, known for its seeds with a characteristic and pleasant odour. In human medicine, coriander is a part of various medicines for the treatment of digestive organs. Phytopathogenic fungi and bacteria are the most common and significant causes of coriander seed disease. The aim of this study was to assess coriander seed health and investigate the possibility of seed disinfection using peppermint (Mentha x piperita) essential oil. The health of the seeds was examined by the filter paper laboratory standard method. Different concentrations (1, 2, 5 and 10%) of peppermint essential oil were used for seed disinfection. The composition of the essential oil was determined by gas chromatographic analysis. The results of seed health analysis showed the presence of a fungus after seven days of incubation in a humid chamber at room temperature. The fungus was identified as Alternaria sp. (sect. Alternaria) based on the symptoms and macroscopic and microscopic features of the fungus (on seed and potato dextrose agar). Immersion treatment of infected seed in peppermint essential oil reduced the presence of Alternaria sp. on coriander seeds. The highest disease inhibition, 69,33%, namely 58,93% without the influence of the emulsifier, was shown by oil solution of 5%. Stagnation in inhibition was observed with a further increase in oil concentration. The results of this study show the possibility of disinfecting coriander seeds with peppermint essential oil to reduce infection with Alternaria sp.
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- 2021
29. Diverzitet vrsta roda Rhizoctonia u Srbiji
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Bulajić, Aleksandra, Vico, Ivana, Tanović, Brankica, Mitrović, Petar, Aleksić, Goran, Vojvodić, Mira, Bulajić, Aleksandra, Vico, Ivana, Tanović, Brankica, Mitrović, Petar, Aleksić, Goran, and Vojvodić, Mira
- Abstract
U periodu 2013-2020. godine, pregledom 22 useva na 15 lokaliteta i šest partija krompira u Srbiji, ustanovljena je pojava nekroze i truleži korena različitih biljaka. Sakupljeno je 330 uzoraka i dobijeno 177 izolata Rhizoctonia spp., koji su morfološki i molekularno razdvojeni u 11 anastomoznih grupa (AG). Rezultati su potvrđeni sekvenciranjem ITS regiona rDNA, kao i LSU, rpb2, tef-1α, atp6 i β-tubulin genskih markera. Detaljno su okarakterisana 64 izolata i generisano je 77 sekvenci koje su omogućile prvi uvid u diverzitet i strukturu populacije Rhizoctonia spp. u Srbiji. Kao prouzrokovači bolesti u Srbiji javljaju se dvojedarne Rhizoctonia spp. AG-A na jagodi, AG-G na paradajzu, trešnji i pasulju, AG-F na paprici, AG-U na jabuci i Poa sp., a od višejedarnih R. solani potvrđeno je prisustvo AG-2-1 na paprici, AG-2-2 na šećernoj repi, AG-3 na krompiru, AG-4 HGI na mrkvi, paprici, uljanoj repici i kupusu, AG-4 HGII na jabuci, AG-6 na malini, kao i Waitea circinata var. zeae na kupusu i uljanoj repici. Od toga, osam AG dokazano je u Srbiji prvi put ili prvi put na novom domaćinu u Srbiji, a osam AG je prvi put opisano na novim domaćinima u svetu. Filogenetske analize ukazale su na njihove međuodnose, na moguće puteve introdukcije i postojanje varijabilnosti u okviru roda Rhizoctonia u Srbiji. Razvijen je protokol za direktnu detekciju AG primenom specifičnih prajmera, kao i qPCR protokol za specifičnu detekciju R. solani AG-2-2. Ustanovljena je različita virulentnost i krug domaćina pojedinih AG inokulacijama 14 biljaka iz osam familija. Identifikovan je izolat Trichoderma harzianum sa agresivnošću prema različitim AG u in vitro i in vivo eksperimentima. Dobijeni rezultati pokazuju da su vrste roda Rhizoctonia veoma značajna i raznovrsna grupa patogena u Srbiji.
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- 2021
30. The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain
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Savić, Iva, Savić, Iva, Nikolić, Milica, Vico, Ivana, Mladenović-Drinić, Snežana, Duduk, Natasa, Stanković, Slavica, Savić, Iva, Savić, Iva, Nikolić, Milica, Vico, Ivana, Mladenović-Drinić, Snežana, Duduk, Natasa, and Stanković, Slavica
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During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) from 20 locations in Serbia. After isolation, fungi were cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based on the in situ identification on PDA, 36 isolates of the section Liseola were selected for further analyses. The pathogenicity of all isolates was confirmed on wheat leaves. The virulence of isolates was determined by measuring the lengths of spots formed at the inoculation leaf site. In order to prove the presence of the species Fusarium verticillioides, a pair of primers FV-F2/FV-R was used. This pair of primers amplifies the sequence of the gaoB gene, and it proved to be specific for the stated species. Moreover, for the same purpose, a pair of primers VER1-VER2 based on the calmodulin partial gene was used. The reference isolate RBG 1603 Q27 was used as a positive control. The pair of primers VER1-VER2 produced a band of the expected size - 578 bp in 18 isolates, while using FV-F2/FV-R, a 370bp long band confirmed the presence of F. verticillioides in 16 samples. Sixteen out of 18 isolates verified with VER1-VER2 were also identified as F. verticillioides with FV-FS/FV-R. No amplification was observed in a negative control., Tokom perioda od 2014. do 2017. godine sakupljani su uzorci pšenice sa fuzarioznih klasova sa 20 lokacija u Srbiji. Nakon izolacije, gljive su gajene na KDA podlozi 7 dana u termostatu na temperaturi od 25oC. Na osnovu identifikacije in situ na KDA (krompir dekstrozni agar), 36 izolata sekcije Liseola odabrano je za dalje analize. Potvrđena je patogenost svih izolata na listovima pšenice. Virulentnost izolata utvrđena je merenjem dužina formirane pege na mestu inokulacije. Za dokazivanje prisustva vrste Fusarium verticillioides korišćen je par prajmera FV-F2/FV-R koji umnožava sekvence gaoB gena i koji se pokazao kao specifičan za navedenu vrstu. Takođe, sa istim ciljem korišćen je par prajmera VER1-VER2 dizajniran na osnovu dela genske sekvence za kalmodulin. Kao pozitivna kontrola korišćen je referentni izolat RBG 1603 Q27. Par prajmera VER1-VER2 obrazovao je traku očekivane veličine (578bp) kod 18 izolata, dok je pomoću para prajmera FV-F2/FV-R traka dužine 370bp potvrdila prisustvo F. verticillioides kod 16 izolata. Šesnaest od 18 izolata koji su identifikovani VER1-VER2 parom, takođe su identifikovani i FV-FS/FV-R prajmerima. U negativnoj kontroli nije bilo amplifikacije.
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- 2021
31. Waitea circinata var. zeae - prouzrokovač truleži korena kupusa i uljane repice u Srbiji
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Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
- Abstract
Kupus i uljana repica su dva vrlo značajna useva u našoj zemlji i njihova proizvodnja ugrožena je brojnim patogenim organizmima. Višejedarne anamorfne Rhizoctonia spp. sa teleomorfom opisanim kao Waitea circinata su biljni patogeni koji se dugo održavaju u zemljištu i rasprostranjeni su i vrlo štetni širom sveta. Od pet opisanih varijeteta W. circinata var. zeae je najrasprostranjeniji. Za sada poznat krug domaćina W. circinata var. zeae obuhvata monokotiledone i mali broj dikotiledonih biljaka, među kojima soju, pasulj, grašak, šećernu repu i mrkvu.
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- 2021
32. Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape
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Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
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Cabbage, a widely used and popular vegetable, and oilseed rape, the second most valuable oilseed crop in the world, are two important species from the Brassicaceae family. Two geographically separated outbreaks of cabbage and oilseed rape root rot with estimated incidence of 15 and 20%, respectively, were recorded during 2017 in the Vojvodina region, Serbia. Twelve hyphal-tip isolates were obtained from symptomatic cabbage and oilseed rape plants and identified as Waitea circinata var. zeae based on morphological and molecular features. This indicates that W. circinata var. zeae has expanded its host range to the Brassicaceae family. Sequence analyses of internal transcribed spacer (ITS) and large subunit of the ribosomal DNA, RPB2, and B-tubulin genes revealed the highest similarity with multiple W. circinata var. zeae. Neighbor-joining analyses of ITS sequences resulted in a phylogenetic tree with one welldefined branch of W. circinata var. zeae, with two separate groups. All Serbian isolates and the majority of isolates originating from natural infection of dicotyledonous plants grouped together in group I. Following artificial inoculation, W. circinata var. zeae isolates caused mild to medium root necrosis of seedlings of 2 monocotyledonous and 12 dicotyledonous plant species, implying a wider host range than was known for W. circinata var. zeae. Additionally, this is the first occurrence of W. circinata var. zeae on dicotyledonous host plants in Europe. Because cabbage and oilseed rape are important crops grown worldwide, the occurrence of this new soilborne pathogen with a broad host range imposes the necessity for changes in routine disease control practices, particularly crop rotation.
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- 2021
33. Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia
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Žebeljan, Aleksandra, Žebeljan, Aleksandra, Duduk, Nataša, Vučković, Nina, Jurick II, Wayne M., Vico, Ivana, Žebeljan, Aleksandra, Žebeljan, Aleksandra, Duduk, Nataša, Vučković, Nina, Jurick II, Wayne M., and Vico, Ivana
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Blue mold, caused by Penicillium spp., is one of the most economically important postharvest diseases of pome fruits, globally. Pome fruits, in particular apple, is the most widely grown pome fruit in Serbia, and the distribution of Penicillium spp. responsible for postharvest decay is unknown. A two-year survey was conducted in 2014 and 2015, where four pome fruits (apple, pear, quince, and medlar) with blue mold symptoms were collected from 20 storage locations throughout Serbia. Detailed morphological characterization, analysis of virulence in three apple cultivars, and multilocus phylogeny revealed three main Penicillium spp. in order of abundance: P. expansum, P. crustosum, and P. solitum. Interestingly, P. expansum split into two distinct clades with strong statistical support that coincided with several morphological observations. Findings from this study are significant and showed previously undocumented diversity in blue mold fungi responsible for postharvest decay including the first finding of P. crustosum, and P. solitum as postharvest pathogens of quince and P. crustosum of medlar fruit in the world, and P. expansum of quince in Serbia. Data from this study provide timely information regarding phenotypic, morphological and genotypic plasticity in P. expansum that will impact the design of species-specific detection tools and guide the development of blue mold management strategies.
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- 2021
34. Waitea circinata var. zeae Causing Root Rot of Cabbage and Oilseed Rape
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Vojvodić, Mira, primary, Tanović, Brankica, additional, Mitrović, Petar, additional, Vico, Ivana, additional, and Bulajić, Aleksandra, additional
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- 2021
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35. Identification of binucleate rhizoctonia spp. ag-a from strawberry and multinucleate R. solani ag-2-2 from sugar beet in Serbia
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Vojvodić, Mira, Tanović, Brankica, Mitrović, Petar, Vico, Ivana, Lazić, Dejan, Stevanović, Miloš, and Bulajić, Aleksandra
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strawberries ,isolates ,fungi ,food and beverages ,binucleate ,inoculation ,identification ,sugar beet ,Rhizoctonia - Abstract
Rhizoctonia spp. comprise soil-borne fungi widely distributed in temperate climate worldwide which are annually causing significant yield loses to a range of cultivated plants. A species status of Rhizoctonia spp. has not yet been established and the isolates are classified based on nuclei status as multinucleate and binucleate Rhizoctonia spp., and further divided into 13 anastomosis groups (AG) (AG-1-13) of multinucleate Rhizoctonia and 23 AG (AG-A-W) of binucleate Rhizoctonia. In this paper we are reporting morphological and molecular features of binucleate Rhizoctonia spp. AG-A causing black root rot on strawberry and multinucleate R. solani AG-2-2 causing root and crown rot of sugar beet. During 2015 and 2016 at several strawberry and sugar beet producing areas, plants expressing prominent root and crown necrosis and rot have been observed with disease incidence estimated at 20-30%.
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- 2020
36. Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone
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Luković, Jelena, Luković, Jelena, Milijašević-Marčić, Svetlana, Hatvani, Lorant, Kredics, Laszlo, Szucs, Attila, Vagvolgyi, Csaba, Duduk, Nataša, Vico, Ivana, Potocnik, Ivana, Luković, Jelena, Luković, Jelena, Milijašević-Marčić, Svetlana, Hatvani, Lorant, Kredics, Laszlo, Szucs, Attila, Vagvolgyi, Csaba, Duduk, Nataša, Vico, Ivana, and Potocnik, Ivana
- Abstract
Twenty-two strains of Trichoderma spp. (T. harzianum species complex [THSC], Trichoderma aggressivum f. europaeum, Trichoderma pleuroti, and Trichoderma pleuroticola) causing green mold disease on edible mushrooms (button mushroom, shiitake and oyster mushroom), collected during 2004-2018 from four countries (Serbia, North Macedonia, Croatia, and Hungary) were examined. Based on their ITS (internal transcribed spacer) sequences, strains from shiitake mushroom in Serbia were identified as members of the THSC, while in samples obtained from Serbian and North-Macedonian oyster mushroom farms THSC, T. pleuroti and T. pleuroticola were detected, which represent the first findings in the region. In fungicide susceptibility tests, all examined Trichoderma strains were found to be highly sensitive to prochloraz (ED(50)0.4 mu g mL(-1)) and considerably susceptible to metrafenone (ED50 lt 4 mu g mL(-1)). The most sensitive taxon to both fungicides was THSC from oyster mushroom. The toxicity of metrafenone was satisfying and strains from oyster mushroom showed the highest sensitivity (ED50 lt 1.43 mu g mL(-1)), while strains originating from button mushroom and shiitake displayed similar susceptibilities (ED50 lt 3.64 mu g mL(-1)). After additional in vivo trials, metrafenone might also be recommended for the control of green mold disease in mushroom farms.
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- 2020
37. Profiling changes in primary metabolites and antioxidants during apple fruit decay caused by Penicillium crustosum
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Žebeljan, Aleksandra, primary, Vico, Ivana, additional, Duduk, Nataša, additional, Žiberna, Bojana, additional, and Krajnc, Andreja Urbanek, additional
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- 2021
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38. The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain
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Savic, Iva, primary, Nikolic, Milica, additional, Nikolic, Ana, additional, Vico, Ivana, additional, Mladenovic-Drinic, Snezana, additional, Duduk, Natasa, additional, and Stankovic, Slavica, additional
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- 2021
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39. Sensitivity of Trichoderma strains from edible mushrooms to the fungicides prochloraz and metrafenone
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Luković, Jelena, primary, Milijašević-Marčić, Svetlana, additional, Hatvani, Lóránt, additional, Kredics, László, additional, Szűcs, Attila, additional, Vágvölgyi, Csaba, additional, Duduk, Nataša, additional, Vico, Ivana, additional, and Potočnik, Ivana, additional
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- 2020
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40. Diversity of Botryosphaeriaceae and Diaporthe Species Associated with Postharvest Apple Fruit Decay in Serbia.
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Vučković, Nina, Vico, Ivana, Duduk, Bojan, and Duduk, Nataša
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BOTRYOSPHAERIACEAE , *FRUIT , *PHYTOPATHOGENIC microorganisms , *SPECIES , *QUINCE , *DEAD trees - Abstract
Family Botryosphaeriaceae and the genus Diaporthe (family Diaporthaceae) represent diverse groups of plant pathogens, which include causal agents of leaf spot, shoot blight, branch and stem cankers, dieback, and pre- and postharvest apple fruit decay. Apple fruit with symptoms of light to dark brown decay were collected during and after harvest from 2016 to 2018. Thirty selected isolates, on which pathogenicity was confirmed, were identified and characterized based on multilocus phylogeny and morphology. Five species from the family Botryosphaeriaceae and two from the genus Diaporthe (fam. Diaporthaceae) were discovered. The most commonly isolated was Diplodia seriata followed by Botryosphaeria dothidea. In this work, Diaporthe rudis is described as a new postharvest pathogen of apple fruit. Diplodia bulgarica, Diplodia sapinea, Neofusicoccum yunnanense, and Diaporthe eres are initially described as postharvest apple and D. sapinea as postharvest quince and medlar fruit pathogens in Serbia. Because species of the family Botryosphaeriaceae and the genus Diaporthe are known to cause other diseases on their hosts, have an endophytic nature, and have a wide host range, findings from this study imply that they may become a new challenge for successful fruit production. [ABSTRACT FROM AUTHOR]
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- 2022
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41. Chamomile Floricolous Downy Mildew Caused by Peronospora radii
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Duduk, Bojan, primary, Duduk, Nataša, additional, Vico, Ivana, additional, Stepanović, Jelena, additional, Marković, Tatjana, additional, Rekanović, Emil, additional, Kube, Michael, additional, and Radanović, Dragoja, additional
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- 2019
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42. First Report of Diplodia seriata Causing Postharvest Rot of Quince Fruit in Serbia
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Vico, Ivana, Vico, Ivana, Žebeljan, Aleksandra, Vucković, N., Vasić, M., Duduk, Nataša, Vico, Ivana, Vico, Ivana, Žebeljan, Aleksandra, Vucković, N., Vasić, M., and Duduk, Nataša
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- 2017
43. Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions
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Žebeljan, Aleksandra, Žebeljan, Aleksandra, Vico, Ivana, Duduk, Nataša, Ziberna, Bojana, Krajnc, Andreja Urbanek, Žebeljan, Aleksandra, Žebeljan, Aleksandra, Vico, Ivana, Duduk, Nataša, Ziberna, Bojana, and Krajnc, Andreja Urbanek
- Abstract
Penicillium expansum-infected apple fruit resulted in a disrupted balance of primary metabolites and impacted the ascorbate-glutathione cycle. The initial decline in fructose, malic acid, shikimic acid and total ascorbate content (6 hpi) was followed by a significant increase in malic acid (24 hpi) and total glutathione (3 dpi). Significant increase in sucrose was accompanied by a decline in glucose and ascorbate content (5 dpi). Total glutathione and shikimic acid gradually deteriorated with symptom development. A negative correlation was found between sucrose and ascorbate-glutathione pool. Other correlations were strong amongst other metabolites and lesion size indicating that they may be involved or important for apple fruit decay.
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- 2019
44. Conventional and real-time PCR assays for detection and identification of Rhizoctonia solani AG-2-2, the causal agent of root rot of sugar beet
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Vojvodić, Mira, Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, Bulajić, Aleksandra, Vojvodić, Mira, Vojvodić, Mira, Lazić, Dejan, Mitrović, Petar, Tanović, Brankica, Vico, Ivana, and Bulajić, Aleksandra
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Soil-borne fungi belonging to the genus Rhizoctonia are considered to be among the most destructive sugar beet pathogens. Although multinucleate R. solani AG-2-2 is frequently detected as the main causal agent of root rot of sugar beet worldwide, several binucleate (AG-A, AG-E and AG-K) and multinucleate Rhizoctonia (R. solani AG-4, AG-5 and AG-8) have also been included in the disease complex. Due to their soil-borne nature and wide host range, the management of Rhizoctonia root rot of sugar beet is highly demanding. Identification of Rhizoctonia AG associated with root rot of sugar beet is the essential first step in determining a successful disease management strategy. In this paper we report a highly specific and sensitive real-time PCR protocol for detection of R. solani AG-2-2 which showed a high level of specificity after testing against 10 different anastomosis groups and subgroups, including AG-2-1 as the most closely related. Moreover, a similar conventional PCR assay showed the same specificity but proved to be at least a 100 times less sensitive. Future research will include further testing and adaptation of this protocol for direct detection and quantification of R. solani AG-2-2 in different substrates, including plant tissue and soil samples., Smatra se da su gljive iz roda Rhizoctonia koje se održavaju u zemljištu, najdestruktivniji patogeni šećerne repe. Mada je širom sveta višejedarna R. solani AG-2-2 najčešće označena kao najvažniji prouzrokovač truleži korena šećerne repe, nekoliko dvojedarnih (AG-A, AG-E i AG-K) kao i višejedarnih Rhizoctonia (R. solani AG-4, AG-5 i AG-8) takođe mogu da učestvuju u kompleksu bolesti. Kako se održavaju u zemljištu i imaju širok krug domaćina, suzbijanje vrsta Rhizoctonia koje izazivaju trulež korena šećerne repe veoma je zahtevno. Identifikacija anastomoznih grupa prouzrokovača oboljenja predstavlja neophodan prvi korak u uspostavljanju uspešnog suzbijanja. U ovom radu razvijen je visokospecifičan i osetljiv real-time PCR protokol za detekciju i identifikaciju R. solani AG-2-2 koji se pokazao kao visokospecifičan nakon testiranja koja su obuhvatila 10 različitih AG grupa i podgrupa, uključujući i AG-2-1 kao najsrodniju. Sličan konvencionalni PCR protokol ispoljio je istu specifičnost, ali i najmanje 100 × manju osetljivost. Buduća istraživanja uključiće dalje testiranje i adaptaciju ovog protokola za direktnu detekciju i kvantifikaciju R. solani AG-2-2 u različitim uzorcima, uključujući biljno tkivo i zemljište.
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- 2019
45. Chamomile Floricolous Downy Mildew Caused by Peronospora radii
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Duduk, Bojan, Duduk, Bojan, Duduk, Nataša, Vico, Ivana, Stepanović, Jelena, Marković, Tatjana, Rekanović, Emil, Kube, Michael, Radanović, Dragoja, Duduk, Bojan, Duduk, Bojan, Duduk, Nataša, Vico, Ivana, Stepanović, Jelena, Marković, Tatjana, Rekanović, Emil, Kube, Michael, and Radanović, Dragoja
- Abstract
Floricolous downy mildews (Peronospora, oomycetes) are a small, monophyletic group of mostly inconspicuous plant pathogens that induce symptoms exclusively on flowers. Characterization of this group of pathogens, and information about their biology, is particularly sparse. The recurrent presence of a disease causing flower malformation which, in turn, leads to high production losses of the medicinal herb Matricaria chamomilla in Serbia has enabled continuous experiments focusing on the pathogen and its biology. Peronospora radii was identified as the causal agent of the disease, and morphologically and molecularly characterized. Diseased chamomile flowers showed severe malformations of the disc and ray florets, including phyllody and secondary inflorescence formation, followed by the onset of downy mildew. Phylogeny, based on internal transcribed spacer and cox2, indicates clustering of the Serbian P. radii with other P. radii from chamomile although, in cox2 analyses, they formed a separate subcluster. Evidence pointing to systemic infection was provided through histological and molecular analyses, with related experiments validating the impact of soilborne and blossom infections. This study provides new findings in the biology of P. radii on chamomile, thus enabling the reconstruction of this floricolous Peronospora species' life cycle.
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- 2019
46. Bull's eye rot of apple fruit caused by Neofabraea alba
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Vico, Ivana, Vico, Ivana, Duduk, Nataša, Vasić, M., Žebeljan, Aleksandra, Radivojević, D., Vico, Ivana, Vico, Ivana, Duduk, Nataša, Vasić, M., Žebeljan, Aleksandra, and Radivojević, D.
- Abstract
Bull's eye rot caused by Neofabraea spp. is an important postharvest disease of apple fruit worldwide. Four species of the genus are known to cause this disease among which Neofabraea alba is the main pathogen causing bull's eye rot in continental Europe. Typical symptoms of the disease were observed in a local market in Belgrade, Serbia in March 2015. Circular lesions, slightly sunken light brown to dark brown with a lighter brown to tan center and a darker outer ring were present on 'Golden Delicious' apples. Decayed tissue was firm. The aim of this study was to identify the causal agent of bull's eye rot of apple fruit based on morphological and molecular characteristics of the pathogen. Two fungal isolates were obtained using standard laboratory procedure and their pathogenicity was tested by wound inoculation of healthy 'Idared' apple fruit. Seven days post inoculation lesions up to 23 cm in diameter developed on inoculated fruit while control fruit remained healthy. The isolates were preliminary identified based on colony morphology. They formed round, slow growing colonies on potato dextrose agar (PDA) with initially white mycelium which turned pinkish-brown with time. Cylindrical to curved-fusiform macroconidia were formed. Microconidia were not observed. Species level identification was completed by sequence analysis of the partial beta-tubulin gene. MegaBLAST analysis of the obtained nucleotide sequences (622 nt) revealed 100% and 99% similarity with several Neofabraea alba sequences deposited in GenBank. Based on morphological characteristics and the partial sequence analysis of p-tubulin gene, Neofabraea alba (Guthrie) Verkley was identified as the causal agent of bull's eye rot of apple fruit in Serbia.
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- 2016
47. Dynamic changes in common metabolites and antioxidants during Penicillium expansum-apple fruit interactions
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Žebeljan, Aleksandra, primary, Vico, Ivana, additional, Duduk, Nataša, additional, Žiberna, Bojana, additional, and Urbanek Krajnc, Andreja, additional
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- 2019
- Full Text
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48. Distribution and Characterization of Monilinia spp. Causing Apple Fruit Decay in Serbia
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Vasić, Miljan, Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., II, Duduk, Nataša, Vasić, Miljan, Vasić, Miljan, Vico, Ivana, Jurick, Wayne M., II, and Duduk, Nataša
- Abstract
Brown rot, caused by Monilinia spp., is an economically important pre and postharvest disease of pome and stone fruits worldwide. In Serbia, apple is the most widely grown pome fruit, and the distribution of economically important Monilinia spp. responsible for apple brown rot is unknown. Hence, we conducted a three year survey, from 2010 to 2012, where 349 isolates were obtained from six orchards and four storage facilities from five different apple cultivars with brown rot symptoms. Morphological characterization of the isolates, multiplex PCR, and phylogenetic analysis revealed four species: M. fructigena, M. laxa, M. fructicola, and Monilia polystroma. All species were found in the orchard and in storage, with M. fructigena predominating, followed by M. polystroma. Representative isolates were analyzed in vitro and in vivo where differences in growth rate, sporulation, and virulence on apple fruit were observed. Findings from this investigation demonstrate diversity in the species responsible for pre and postharvest apple brown rot, which has significant implications for pathogen detection and for developing disease-specific management strategies.
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- 2018
49. Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia
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Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, Bulajić, Aleksandra, Vojvodić, Mira, Vojvodić, Mira, Tanović, Brankica, Mihajlović, Milica, Mitrović, Petar, Vico, Ivana, and Bulajić, Aleksandra
- Abstract
Strawberry production is a popular, fast-growing agricultural business in Serbia. Its cultivar selection has been changing fast, following market demands. One of the limiting factors of strawberry production worldwide is black root rot, primarily caused by binucleate Rhizoctonia. Recently, outbreaks of black root rot of strawberry have occurred in Serbia and the estimated disease incidence was up to 30%. Isolates of binucleate Rhizoctonia AG-A were recovered from symptomatic strawberry plants, and characterized on the bases of morphological, molecular and pathogenic features. Despite their uniform morphological characteristics, the isolates demonstrated genetic variability within ITS rDNA, grouping into three different phylogenetic sub-clusters which comprise AG-A isolates originating from Italy, Israel, Japan and the USA. The binucleate Rhizoctonia AG-A from Serbia exhibited uniform virulence on strawberry after inoculation of daughter plants and detached leaf petioles, as well as on seedlings of bean, carrot and sunflower, while they were non-pathogenic to wheat, maize, tomato, pepper, tobacco, cucumber, lettuce, peas, cabbage, rapeseed and sugar beet., Proizvodnja jagode je popularna grana poljoprivrede u Srbiji koja se brzo razvija. Sastav sorti uključenih u proizvodnju podleže stalnim promenama u skladu sa zahtevima tržišta. Jedan od ograničavajućih faktora u proizvodnji jagode širom sveta je crna trulež korena, bolest koju najčešće izazivaju dvojedarne vrste roda Rhizoctonia. U Srbiji je poslednjih godina nekoliko puta zabeležena pojava crne truleži jagode uz učestalost koja je iznosila do 30%. Iz biljaka jagode sa simptomima izdvojeni su izolati dvojedarne Rhizoctonia AG-A i okarakterisani na osnovu morfološih i molekularnih osobina i patogenosti. Uprkos uniformnim morfološkim osobinama, izolati su ispoljili genetičku varijabilnost na osnovu sekvence ITS rDNK, grupišući se u tri različita filogentska sub-klastera koji su obuhvatali isolate iz Italije, Izraela, Japana i SAD. Izolati dvojedarnih Rhizoctonia AG-A poreklom iz Srbije ispoljili su uniformnu virulentnost na jagodi nakon inokulacija živića i fragmenata lisnih peteljki, kao i na sejancima pasulja, mrkve i suncokreta, dok nisu bili patogeni za pšenicu, kukuruz, paradajz, papriku, duvan, krastavac, salatu, grašak, kupus i šećernu repu.
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- 2018
50. Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk
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Luković, Jelena, Luković, Jelena, Stepanović, Miloš, Todorović, Biljana, Milijašević-Marčić, Svetlana, Duduk, Nataša, Vico, Ivana, Potočnik, Ivana, Luković, Jelena, Luković, Jelena, Stepanović, Miloš, Todorović, Biljana, Milijašević-Marčić, Svetlana, Duduk, Nataša, Vico, Ivana, and Potočnik, Ivana
- Abstract
Antifungal activity of two essential oils, cinnamon (Cinnamomum verum J. Presl) and clove (Syzygium aromaticum (L.) Merrill & Perry), was evaluated against Cladobotryum dendroides (Bull.) W. Gams & Hooz, and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, the causal agents of cobweb and dry bubble disease of cultivated mushroom. Inhibitory and fungicidal activity of the selected essential oils was assayed using three methods: microdilution, macrodilution fumigant and macrodilution contact method. Comparing all three methods, clove essential oil showed stronger activity than cinnamon against both fungi, having minimum inhibitory concentration (MIC) at the lowest concentrations tested (1.56, 0.02 and 0.1 µl ml-1, respectively). However, cinnamon oil was more toxic to L. fungicola var. fungicola then to C. dendroides in all three methods. Both oils exhibited stronger antifungal effects when used in the macrodilution fumigant than in contact method. The results showed that both cinnamon and clove essential oils have the potential for further in vivo experiments against L. fungicola var. fungicola and C. dendroides and indicated a possible use of these oils in integrated disease management in mushrooms., Ispitana je antifungalna aktivnost etarskih ulja cimeta i karanfilića prema Cladobotryum dendroides (Bull.) W. Gams & Hooz, i Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, prouzrokovačima paučinaste plesni i suve truleži šampinjona. Antifungalna aktivnost odabranih etarskih ulja testirana je primenom tri različite metode: mikrodilucione, makrodilucione fumigantne i makrodilucione kontaktne metode. Etarsko ulje karanfilića je ispoljilo jači efekat od ulja cimeta na obe gljive primenom sve tri metode, sa minimalnim inhibitornim koncentrecijama pri najnižim testiranim koncentracijama (1,56, 0,02 i 0,1 µl ml-1). Ulje cimeta je pokazalo veću toksičnost prema L. fungicola var. fungicola nego prema C. dendroides. Ispitivana etarska ulja ispoljila su jače antifungalno dejstvo primenjena fumigantno nego kontaktno u makrodilucionoj metodi. Antifungalno delovanje etarskih ulja cimeta i karanfilića in vitro, ukazuje na potencijal za dalja ispitivanja njihove efikasnosti in vivo, kao i mogućnosti uključivanja ovih etarskih ulja u integralnu zaštitu jestivih gljiva od bolesti prouzrokovanih mikopatogenim gljivama L. fungicola var. fungicola i C. dendroides.
- Published
- 2018
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