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3. Biomarkers of peanut allergy in children over time.

Author

Foong, Ru‐Xin, Du Toit, George, van Ree, Ronald, Bahnson, Henry T., Radulovic, Suzana, Craven, Jo, Kwok, Matthew, Jama, Zainab, Versteeg, Serge A., Brough, Helen A., Logan, Kirsty, Perkin, Michael R., Flohr, Carsten, Lack, Gideon, and Santos, Alexandra F.

Subjects
PEANUT allergy, MAST cells, FOOD allergy, CELL anatomy, SKIN tests
Abstract

Background: Various biomarkers are used to define peanut allergy (PA). We aimed to observe changes in PA resolution and persistence over time comparing biomarkers in PA and peanut sensitised but tolerant (PS) children in a population‐based cohort. Methods: Participants were recruited from the EAT and EAT‐On studies, conducted across England and Wales, and were exclusively breastfeed babies recruited at 3 months old and followed up until 7–12 years old. Clinical characteristics, skin prick test (SPT), sIgE to peanut and peanut components and mast cell activation tests (MAT) were assessed at 12 months, 36 months and 7–12 years. PA status was determined at the 7–12 year time point. Results: The prevalence of PA was 2.1% at 7–12 years. Between 3 and 7–12 year, two children developed PA and one outgrew PA. PA children had larger SPT, higher peanut‐sIgE, Ara h 2‐sIgE and MAT (all p <.001) compared to PS children from 12 months onwards. SPT, peanut‐sIgE, Ara h 2‐sIgE and MAT between children with persistent PA, new PA, outgrown PA and PS were statistically significant from 12 months onwards (p <.001). Those with persistent PA had SPT, peanut‐sIgE and Ara h 2‐sIgE that increased over time and MAT which was highest at 36 months. New PA children had increased SPT and peanut‐sIgE from 36 months to 7–12 years, but MAT remained low. PS children had low biomarkers across time. Conclusions: In this cohort, few children outgrow or develop new PA between 36 months and 7–12 years. Children with persistent PA have raised SPT, peanut‐sIgE, Ara h 2‐sIgE and MAT evident from infancy that consistently increase over time. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Walnut Allergy Across Europe: Distribution of Allergen Sensitization Patterns and Prediction of Severity

Author

Lyons, Sarah A., Datema, Mareen R., Le, Thuy-My, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frédéric, Clausen, Michael, Dubakiene, Ruta, Fernández-Perez, Cristina, Fritsche, Philipp, Gislason, David, Hoffmann-Sommergruber, Karin, Jedrzejczak-Czechowicz, Monika, Jongejan, Laurian, Kowalski, Marek L., Kralimarkova, Tanya Z., Lidholm, Jonas, Papadopoulos, Nikolaos G., Pontoppidan, Bo, Popov, Todor A., Prado, Nayade del, Purohit, Ashok, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Vassilopoulou, Emilia, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Welsing, Paco M.J., Mills, E.N. Clare, Ballmer-Weber, Barbara K., Knulst, André C., Fernández-Rivas, Montserrat, and Van Ree, Ronald

Published
2021
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6. Factors influencing adherence in a trial of early introduction of allergenic food

Author

Young, Louise, Offord, Victoria, DeSousa, Mary, Cullen, Jason, Taylor, Katherine, Tseng, Anna, Raji, Bunmi, Byrom, Sarah, Regis, Gillian, Bigwood, Charlie, Stedman, Charlotte, Tonner, Sharon, Banks, Emily, Kahnum, Yasmin, Babic, Rachel, Stockwell, Ben, Thompson, Erin, Wheatley, Lorna, Patkunam, Devi, Perkin, Michael R., Bahnson, Henry T., Logan, Kirsty, Marrs, Tom, Radulovic, Suzana, Knibb, Rebecca, Craven, Joanna, Flohr, Carsten, Mills, E.N., Versteeg, Serge A., van Ree, Ronald, and Lack, Gideon

Published
2019
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7. Efficacy of the Enquiring About Tolerance (EAT) study among infants at high risk of developing food allergy

Author

Young, Louise, Offord, Victoria, DeSousa, Mary, Cullen, Jason, Taylor, Katherine, Tseng, Anna, Raji, Bunmi, Byrom, Sarah, Regis, Gillian, Bigwood, Charlie, Stedman, Charlotte, Tonner, Sharon, Banks, Emily, Kahnum, Yasmin, Babic, Rachel, Stockwell, Ben, Thompson, Erin, Wheatley, Lorna, Patkunam, Devi, Richards, Kerry, Pietraszewicz, Ewa, Stephens, Alick, Sudra, Asha, Turcanu, Victor, Perkin, Michael R., Logan, Kirsty, Bahnson, Henry T., Marrs, Tom, Radulovic, Suzana, Craven, Joanna, Flohr, Carsten, Mills, E.N., Versteeg, Serge A., van Ree, Ronald, and Lack, Gideon

Published
2019
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8. Plant‐produced Der p 2‐bearing bioparticles activate Th1/Treg‐related activation patterns in dendritic cells irrespective of the allergic background.

Author

Busold, Stefanie, Aglas, Lorenz, Menage, Charlotte, Desgagnés, Réjean, Faye, Loïc, Fitchette, Anne‐Catherine, de Jong, Esther C., Martel, Caroline, Stigler, Maria, Catala‐Stordeur, Virginie, Tropper, Guy, Auger, Lydia, Morel, Bertrand, Versteeg, Serge A., Vézina, Louis‐P, Gomord, Véronique, Layhadi, Janice A., Shamji, Mohamed, Geijtenbeek, Teunis B. H., and van Ree, Ronald

Subjects
DENDRITIC cells, ALLERGY desensitization, CELL aggregation, MEDICAL research, SCHOLARSHIPS
Abstract

This article discusses the use of plant-produced bioparticles to deliver allergens for immunotherapy. The researchers compared the allergenic and immunogenic potency of soluble and particulate allergen presentation. They found that the bioparticles were hypoallergenic and induced immunomodulatory responses in dendritic cells, regardless of the allergic background. The study suggests that these bioparticles could be used to redirect the immune response towards a more balanced Th1/Treg response, improving the safety and efficacy of allergen immunotherapy. [Extracted from the article]

Published
2024
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9. Unique allergen-specific human IgE monoclonal antibodies derived from patients with allergic disease

Author

Smith, Bryan R. E., primary, Reid Black, Kristina, additional, Bermingham, Max, additional, Agah, Sayeh, additional, Glesner, Jill, additional, Versteeg, Serge A., additional, van Ree, Ronald, additional, Pena-Amelunxen, Glorismer, additional, Aglas, Lorenz, additional, Smith, Scott A., additional, Pomés, Anna, additional, and Chapman, Martin D., additional

Published
2023
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10. Corrigendum: Biological activity of human IgE monoclonal antibodies targeting Der p 2, Fel d 1, Ara h 2 in basophil mediator release assays

Author

Pena-Castellanos, Glorismer, primary, Smith, Bryan R. E., additional, Pomés, Anna, additional, Smith, Scott A., additional, Stigler, Maria A., additional, Widauer, Hannah L., additional, Versteeg, Serge A., additional, van Ree, Ronald, additional, Chapman, Martin D., additional, and Aglas, Lorenz, additional

Published
2023
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11. Variation in IgE binding potencies of seven Artemisia species depending on content of major allergens

Author

Zhao, Lan, Fu, Wanyi, Gao, Biyuan, Liu, Yi, Wu, Shandong, Chen, Zhi, Zhang, Xianqi, Wang, Huiying, Feng, Yan, Wang, Xueyan, Wang, Hongtian, Lan, Tianfei, Liu, Meiling, Wang, Xuefeng, Sun, Yuemei, Luo, Fangmei, Gadermaier, Gabriele, Ferreira, Fatima, Versteeg, Serge A., Akkerdaas, Jaap H., Wang, Deyun, Valenta, Rudolf, Vrtala, Susanne, Gao, Zhongshan, and van Ree, Ronald

Published
2020
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13. Changes in IgE sensitization and total IgE levels over 20 years of follow-up

Author

Amaral, André F.S., Newson, Roger B., Abramson, Michael J., Antó, Josep M., Bono, Roberto, Corsico, Angelo G., de Marco, Roberto, Demoly, Pascal, Forsberg, Bertil, Gislason, Thorarinn, Heinrich, Joachim, Huerta, Ismael, Janson, Christer, Jõgi, Rain, Kim, Jeong-Lim, Maldonado, José, Martinez-Moratalla Rovira, Jesús, Neukirch, Catherine, Nowak, Dennis, Pin, Isabelle, Probst-Hensch, Nicole, Raherison-Semjen, Chantal, Svanes, Cecilie, Urrutia Landa, Isabel, van Ree, Ronald, Versteeg, Serge A., Weyler, Joost, Zock, Jan-Paul, Burney, Peter G.J., and Jarvis, Deborah L.

Published
2016
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14. Hazelnut allergy across Europe dissected molecularly: A EuroPrevall outpatient clinic survey

Author

Datema, Mareen R., Zuidmeer-Jongejan, Laurian, Asero, Riccardo, Barreales, Laura, Belohlavkova, Simona, de Blay, Frédéric, Bures, Peter, Clausen, Michael, Dubakiene, Ruta, Gislason, David, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L., Knulst, André C., Kralimarkova, Tanya, Le, Thuy-My, Lovegrove, Alison, Marsh, Justin, Papadopoulos, Nikolaos G., Popov, Todor, del Prado, Náyade, Purohit, Ashok, Reese, Gerald, Reig, Isabel, Seneviratne, Suranjith L., Sinaniotis, Athanasios, Versteeg, Serge A., Vieths, Stefan, Zwinderman, Aeilko H., Mills, Clare, Lidholm, Jonas, Hoffmann-Sommergruber, Karin, Fernández-Rivas, Montserrat, Ballmer-Weber, Barbara, and van Ree, Ronald

Published
2015
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17. Surface Expression of Major Allergens on Plant-Made eBioparticle Combines Hypo-Allergenicity with Potent Immune Activation

Author

Busold, Stefanie, primary, Aglas, Lorenz, additional, Menage, Charlotte, additional, Auger, Lydia, additional, Desgagnés, Réjean, additional, Faye, Loïc, additional, Fitchette, Anne-Catherine, additional, de Jong, Esther, additional, Martel, Caroline, additional, Stigler, Maria, additional, Stordeur, Virginie, additional, Tropper, Guy, additional, Versteeg, Serge, additional, Vézina, Louis, additional, Gomord, Véronique, additional, Geijtenbeek, Teunis, additional, and van Ree, Ronald, additional

Published
2022
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20. Association of frequent moisturizer use in early infancy with the development of food allergy

Author

Perkin, Michael R., Logan, Kirsty, Marrs, Tom, Radulovic, Suzana, Craven, Joanna, Boyle, Robert J., Chalmers, Joanne R., Williams, Hywel C., Versteeg, Serge A., Van Ree, Ronald, Lack, Gideon, and Flohr, Carsten

Abstract

BackgroundFood allergy is thought to develop through transcutaneous sensitization, especially in the presence of skin barrier impairment and inflammation. Regular moisturizer application to infant skin could potentially promote transcutaneous sensitization and the development of food allergy.ObjectivesWe tested this hypothesis in the Enquiring About Tolerance (EAT) study population.MethodsThe EAT study was a population-based randomized clinical trial conducted from January 15, 2008, to August 31, 2015, and recruited 1303 exclusively breastfed 3-month-old infants and their families from England and Wales. At enrollment at 3 months, families completed a questionnaire that included questions about frequency and type of moisturizer applied, use of corticosteroid creams, and parental report of dry skin or eczema. Infants were examined for visible eczema at the enrollment visit.ResultsA statistically significant dose-response relationship was observed between parent-reported moisturization frequency at 3 months of age and the subsequent development of food allergy. Each additional moisturization per week was associated with an adjusted odds ratio of 1.20 (95% CI, 1.13-1.27; P [less than].0005) for developing food allergy. For infants with no visible eczema at the enrollment visit, the corresponding adjusted odds ratio was 1.18 (95% CI, 1.07-1.30; P = .001) and for those with eczema at the enrollment visit, 1.20 (95% CI, 1.11-1.31; P [less than] .0005). Moisturizer frequency showed similar dose-response relationships with the development of both food and aeroallergen sensitization at 36 months.ConclusionsThese findings support the notion that regular application of moisturizers to the skin of young infants may promote the development of food allergy through transcutaneous sensitization.

Published
2021

21. Predicting food allergy: The value of patient history reinforced

Author

Lyons, Sarah A; https://orcid.org/0000-0001-7128-2699, Knulst, André C, Burney, Peter G J, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara K, Barreales, Laura, Bieli, Christian, Clausen, Michael, Dubakiene, Ruta, Fernandez-Perez, Cristina, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L; https://orcid.org/0000-0002-8442-2774, Kummeling, Ischa, Kralimarkova, Tanya, Mustakov, Tihomir B, van Os-Medendorp, Harmieke, Papadopoulos, Nikolaos G, Popov, Todor A, Potts, James, Versteeg, Serge A, Xepapadaki, Paraskevi; https://orcid.org/0000-0001-9204-1923, Welsing, Paco M J, Mills, Clare, van Ree, Ronald, Le, Thuy-My, Lyons, Sarah A; https://orcid.org/0000-0001-7128-2699, Knulst, André C, Burney, Peter G J, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara K, Barreales, Laura, Bieli, Christian, Clausen, Michael, Dubakiene, Ruta, Fernandez-Perez, Cristina, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L; https://orcid.org/0000-0002-8442-2774, Kummeling, Ischa, Kralimarkova, Tanya, Mustakov, Tihomir B, van Os-Medendorp, Harmieke, Papadopoulos, Nikolaos G, Popov, Todor A, Potts, James, Versteeg, Serge A, Xepapadaki, Paraskevi; https://orcid.org/0000-0001-9204-1923, Welsing, Paco M J, Mills, Clare, van Ree, Ronald, and Le, Thuy-My

Abstract

BACKGROUND: EAACI guidelines emphasize the importance of patient history in diagnosing food allergy (FA) and the need for studies investigating its value using standardized allergy-focused questionnaires. OBJECTIVE: To determine the contribution of reaction characteristics, allergic comorbidities and demographics to prediction of FA in individuals experiencing food-related adverse reactions. METHODS: Adult and school-age participants in the standardized EuroPrevall population surveys, with self-reported FA, were included. Penalized multivariable regression was used to assess the association of patient history determinants with "probable" FA, defined as a food-specific case history supported by relevant IgE sensitization. RESULTS: In adults (N = 844), reproducibility of reaction (OR 1.35 [95% CI 1.29-1.41]), oral allergy symptoms (OAS) (4.46 [4.19-4.75]), allergic rhinitis (AR) comorbidity (2.82 [2.68-2.95]), asthma comorbidity (1.38 [1.30-1.46]) and male sex (1.50 [1.41-1.59]) were positively associated with probable FA. Gastrointestinal symptoms (0.88 [0.85-0.91]) made probable FA less likely. The AUC of a model combining all selected predictors was 0.85 after cross-validation. In children (N = 670), OAS (2.26 [2.09-2.44]) and AR comorbidity (1.47 [CI 1.39-1.55]) contributed most to prediction of probable FA, with a combined cross-validation-based AUC of 0.73. When focusing on plant foods, the dominant source of FA in adults, the pediatric model also included gastrointestinal symptoms (inverse association), and the AUC increased to 0.81. CONCLUSIONS: In both adults and school-age children from the general population, reporting of OAS and of AR comorbidity appear to be the strongest predictors of probable FA. Patient history particularly allows for good discrimination between presence and absence of probable plant FA.

Published
2021

22. Predicting food allergy: the value of patient history reinforced

Author

MS Dermatologie/Allergologie, Infection & Immunity, Afdeling Dermatologie/Allergologie, Lab Reumatologie/Klinische Immunologie, Regenerative Medicine and Stem Cells, JC onderzoeksprogramma Methodologie, Lyons, Sarah A, Knulst, André C, Burney, Peter G J, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara K, Barreales, Laura, Bieli, Christian, Clausen, Michael, Dubakiene, Ruta, Fernandez-Perez, Cristina, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L, Kummeling, Ischa, Kralimarkova, Tanya, Mustakov, Tihomir B, van Os-Medendorp, Harmieke, Papadopoulos, Nikolaos G, Popov, Todor A, Potts, James, Versteeg, Serge A, Xepapadaki, Paraskevi, Welsing, Paco M J, Mills, E N Clare, van Ree, Ronald, Le, Thuy-My, MS Dermatologie/Allergologie, Infection & Immunity, Afdeling Dermatologie/Allergologie, Lab Reumatologie/Klinische Immunologie, Regenerative Medicine and Stem Cells, JC onderzoeksprogramma Methodologie, Lyons, Sarah A, Knulst, André C, Burney, Peter G J, Fernandez-Rivas, Montserrat, Ballmer-Weber, Barbara K, Barreales, Laura, Bieli, Christian, Clausen, Michael, Dubakiene, Ruta, Fernandez-Perez, Cristina, Jedrzejczak-Czechowicz, Monika, Kowalski, Marek L, Kummeling, Ischa, Kralimarkova, Tanya, Mustakov, Tihomir B, van Os-Medendorp, Harmieke, Papadopoulos, Nikolaos G, Popov, Todor A, Potts, James, Versteeg, Serge A, Xepapadaki, Paraskevi, Welsing, Paco M J, Mills, E N Clare, van Ree, Ronald, and Le, Thuy-My

Published
2021

25. Estimating the Risk of Severe Peanut Allergy Using Clinical Background and IgE Sensitization Profiles

Author

Datema, Mareen R., primary, Lyons, Sarah A., additional, Fernández-Rivas, Montserrat, additional, Ballmer-Weber, Barbara, additional, Knulst, André C., additional, Asero, Riccardo, additional, Barreales, Laura, additional, Belohlavkova, Simona, additional, de Blay, Frédéric, additional, Clausen, Michael, additional, Dubakiene, Ruta, additional, Fernández-Perez, Cristina, additional, Fritsche, Philipp, additional, Gislason, David, additional, Hoffmann-Sommergruber, Karin, additional, Jedrzejczak-Czechowicz, Monika, additional, Jongejan, Laurian, additional, Kowalski, Marek L., additional, Kralimarkova, Tanya Z., additional, Lidholm, Jonas, additional, Papadopoulos, Nikolaos G., additional, Popov, Todor A., additional, Prado, Nayade del, additional, Purohit, Ashok, additional, Reig, Isabel, additional, Seneviratne, Suranjith L., additional, Sinaniotis, Athanassios, additional, Vassilopoulou, Emilia, additional, Versteeg, Serge A., additional, Vieths, Stefan, additional, Welsing, Paco M. J., additional, Mills, E. N. Clare, additional, Le, Thuy-My, additional, Zwinderman, Aeilko H., additional, and van Ree, Ronald, additional

Published
2021
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27. Additional file 1 of Variation in IgE binding potencies of seven Artemisia species depending on content of major allergens

Author

Zhao, Lan, Wanyi Fu, Biyuan Gao, Liu, Yi, Shandong Wu, Chen, Zhi, Xianqi Zhang, Huiying Wang, Feng, Yan, Xueyan Wang, Hongtian Wang, Tianfei Lan, Meiling Liu, Xuefeng Wang, Yuemei Sun, Fangmei Luo, Gadermaier, Gabriele, Ferreira, Fatima, Versteeg, Serge A., Akkerdaas, Jaap H., Deyun Wang, Valenta, Rudolf, Vrtala, Susanne, Gao, Zhongshan, and Ree, Ronald Van

Abstract

Additional file 1: Table S1. Clinical and demographic data of 150 mugwort pollen-allergic individuals sIgE against mugwort extract (w6), Art v 1 (w231) and Art v 3(w233) determined by ImmunoCAP, ND, not determined. AS asthma; AR allergic rhinitis; C, conjunctivitis; E, eczema. I-1, I-2, I-3, I-4 indicates the patients serum used in ImmunoCAP inhibition assay belonging to four groups of different sensitization patterns (1, Art v 1 and Art v 3 positive; 2, Art v 1 positive, Art v 3 negative; 3, Art v 1 negative, Art v 3 positive; 4, Art v 1 and Art v 3 negative). The 82 patients reported in previously studies17, 20, 28 are indicated by an asterisk. Table S2. GenBank accession numbers for three allergen groups in seven Artemisia species.Table S3. Productivity of the three group allergens purified by specific mAb. Table S4. ImmunoCAP IgE characterization of serum pools from five areas. Figure S1. Six Artemisia species collected from China. Figure S2. SDS-PAGE of natural purified Art v 1, Art v 2 and Art v 3 homologous allergens from six Chinese Artemisia species. a, natural Art v 1 homologues purified by specific mAb A7-G4-E6; b, natural Art v 2 homologues purified by specific mAb C9-C1 shown in six different gels; c, natural Art v 3 homologues purified by specific mAb A2-B8. Figure S3. Mass spectra of natural purified Art v 1(a), Art v 2(b) and Art v 3(c) homologues. The peptides verified by LC–MS/MS are shown in red and highlighted. Figure S4. ELISA quantification of three allergen components in Artemisia spp. pollen. a, Chinese silver mugwort (A. argyi) pollen extract (ArE) in SDS gel and reaction to polyclonal antibodies (pAb) by Western blot; b, ELISA standard curve for Art v 1 allergen (mAb A7-G4-E6 and rabbit pAbs); c, ELISA standard curve for Art v 2 homologous allergen (mAb C9-C1 with rabbit pAbs); d, ELISA standard curve for Art v 3 homologous allergen with two mAbs (mAbs A2-B8 and A9-G10) with representative different isoforms. Figure S5..Inhibition of IgE binding to Art v 1 and Art v 3 with seven pollen extracts using two serum pools. a: inhibition ELISA coated with rArt v 1.0101, serum pool from Datong, Shanxi (nArt v 1: 11 kUA/l); b: inhibition ELISA coated with rArt v 1.0101, serum pool from Yantai, Shandong (nArt v 1: 6.51 kUA/l); c: inhibition ELISA coated with rArt v 3.0201, serum pool from Datong, Shanxi (CAP nArt v 3: 11.27 kUA/l); d: inhibition ELISA coated with rArt v 3.0201, serum pool from Yantai, Shandong (CAP nArt v 3: 9.65 kUA/l). Figure S6. Inhibition of sera pool from Shanxi with extract from different species at 100 μg/ml in ELISA coated with 10 μg/ml of different pollen extracts and mixture. 6-mix, mixture of six Artemisia spp. extract in the same proportions.

Published
2020
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28. Selection of Pru p 3 hypoallergenic peach and nectarine varieties

Author

National Natural Science Foundation of China, Zhejiang Provincial Natural Science Foundation, Shanghai Science and Technology Committee, Jin, Jing, Gao, Ling, Zhao, Lan, Gao, Zhong Shan, Li, Xiong-wei, Xie, Han‐bing, Ni, Jun-bei, Gan, Kexin, Wu, Shan-dong, Ye, Zheng-wen, Luo, Jun, Cao, Ke, Ma, Rui-juan, Chen, Miao-jin, Arús, Pere, Versteeg, Serge A., Wang, Hui-ying, Liu, Mei-ling, Jia, Hui-juan, Ree, Ronald van, National Natural Science Foundation of China, Zhejiang Provincial Natural Science Foundation, Shanghai Science and Technology Committee, Jin, Jing, Gao, Ling, Zhao, Lan, Gao, Zhong Shan, Li, Xiong-wei, Xie, Han‐bing, Ni, Jun-bei, Gan, Kexin, Wu, Shan-dong, Ye, Zheng-wen, Luo, Jun, Cao, Ke, Ma, Rui-juan, Chen, Miao-jin, Arús, Pere, Versteeg, Serge A., Wang, Hui-ying, Liu, Mei-ling, Jia, Hui-juan, and Ree, Ronald van

Published
2020

30. Gut microbiota development during infancy: Impact of introducing allergenic foods

Author

Marrs, Tom, primary, Jo, Jay-Hyun, additional, Perkin, Michael R., additional, Rivett, Damian W., additional, Witney, Adam A., additional, Bruce, Kenneth D., additional, Logan, Kirsty, additional, Craven, Joanna, additional, Radulovic, Suzana, additional, Versteeg, Serge A., additional, van Ree, Ronald, additional, McLean, W. H. Irwin, additional, Strachan, David P., additional, Lack, Gideon, additional, Kong, Heidi H., additional, and Flohr, Carsten, additional

Published
2021
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32. Sensitization to Cor a 9 and Cor a 14 is highly specific for a hazelnut allergy with objective symptoms in Dutch children and adults

Author

Masthoff, Laury J.N., Mattsson, Lars, Zuidmeer-Jongejan, Laurian, Lidholm, Jonas, Andersson, Kerstin, Akkerdaas, Jaap H., Versteeg, Serge A., Garino, Cristiano, Meijer, Yolanda, Kentie, Petra, Versluis, Astrid, den Hartog Jager, Constance F., Bruijnzeel-Koomen, Carla A.F.M., Knulst, André C., van Ree, Ronald, van Hoffen, Els, and Pasmans, Suzanne G.M.A.

Published
2013
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34. Predicting food allergy: The value of patient history reinforced

Author

Lyons, Sarah A., primary, Knulst, André C., additional, Burney, Peter G. J., additional, Fernandez‐Rivas, Montserrat, additional, Ballmer‐Weber, Barbara K., additional, Barreales, Laura, additional, Bieli, Christian, additional, Clausen, Michael, additional, Dubakiene, Ruta, additional, Fernandez‐Perez, Cristina, additional, Jedrzejczak‐Czechowicz, Monika, additional, Kowalski, Marek L., additional, Kummeling, Ischa, additional, Kralimarkova, Tanya, additional, Mustakov, Tihomir B., additional, Os‐Medendorp, Harmieke, additional, Papadopoulos, Nikolaos G., additional, Popov, Todor A., additional, Potts, James, additional, Versteeg, Serge A., additional, Xepapadaki, Paraskevi, additional, Welsing, Paco M. J., additional, Mills, Clare, additional, Ree, Ronald, additional, and Le, Thuy‐My, additional

Published
2020
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35. Cross‐reactive carbohydrate determinant‐specific IgE obscures true atopy and exhibits ⍺‐1,3‐fucose epitope‐specific inverse associations with asthma

Author

Nkurunungi, Gyaviira, primary, Mpairwe, Harriet, additional, Versteeg, Serge A., additional, Diepen, Angela, additional, Nassuuna, Jacent, additional, Kabagenyi, Joyce, additional, Nambuya, Irene, additional, Sanya, Richard E., additional, Nampijja, Margaret, additional, Serna, Sonia, additional, Reichardt, Niels‐Christian, additional, Hokke, Cornelis H., additional, Webb, Emily L., additional, Ree, Ronald, additional, Yazdanbakhsh, Maria, additional, and Elliott, Alison M., additional

Published
2020
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36. Frequency of food allergy in school‐aged children in eight European countries—The EuroPrevall‐iFAAM birth cohort

Author

Grabenhenrich, Linus, primary, Trendelenburg, Valérie, additional, Bellach, Johanna, additional, Yürek, Songül, additional, Reich, Andreas, additional, Fiandor, Ana, additional, Rivero, Daniela, additional, Sigurdardottir, Sigurveig, additional, Clausen, Michael, additional, Papadopoulos, Nikolaos G., additional, Xepapadaki, Paraskevi, additional, Sprikkelman, Aline B., additional, Dontje, Bianca, additional, Roberts, Graham, additional, Grimshaw, Kate, additional, Kowalski, Marek L., additional, Kurowski, Marcin, additional, Dubakiene, Ruta, additional, Rudzeviciene, Odilija, additional, Fernández‐Rivas, Montserrat, additional, Couch, Philip, additional, Versteeg, Serge A., additional, Ree, Ronald, additional, Mills, Clare, additional, Keil, Thomas, additional, and Beyer, Kirsten, additional

Published
2020
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38. Molecular Nutrition & Food Research / Rational design, structure-activity relationship, and immunogenicity of hypoallergenic Pru p 3 variants

Author

Eichhorn, Stephanie, Hörschläger, Angelika, Steiner, Markus, Laimer, Josef, Jensen, Bettina M., Versteeg, Serge A., Pablos, Isabel, Briza, Peter, Jongejan, Laurian, Rigby, Neil, Asturias, Juan A., Portolés, Antonio, Fernandez-Rivas, Montserrat, Papadopoulos, Nikolaos G., Mari, Adriano, Poulsen, Lars K., Lackner, Peter, van Ree, Ronald, Ferreira, Fatima, and Gadermaier, Gabriele

Subjects
Pru p 3, allergen immunotherapy, peach allergies, hypoallergens, lipid transfer proteins
Abstract

Scope Allergies to lipid transfer proteins involve severe adverse reactions; thus, effective and sustainable therapies are desired. Previous attempts disrupting disulfide bonds failed to maintain immunogenicity; thus, the aim is to design novel hypoallergenic Pru p 3 variants and evaluate the applicability for treatment of peach allergy. Methods and results Pru p 3 proline variant (PV) designed using in silico mutagenesis, cysteine variant (CV), and wildtype Pru p 3 (WT) are purified from Escherichia coli. Variants display homogenous and stable protein conformations with an altered secondary structure in circular dichroism. PV shows enhanced longterm storage capacities compared to CV similar to the highly stable WT. Using sera of 33 peach allergic patients, IgEbinding activity is reduced by 97% (PV) and 71% (CV) compared to WT. Both molecules show strong hypoallergenicity in Pru p 3 ImmunoCAP crossinhibition and histamine release assays. Immunogenicity of PV is demonstrated with a phosphatebased adjuvant formulation in a mouse model. Conclusions An in silico approach is used to generate a PV without targeting disulfide bonds, T cell epitopes, or previously reported IgE epitopes of Pru p 3. PV is strongly hypoallergenic while structurally stable and immunogenic, thus representing a promising candidate for peach allergen immunotherapy. FAST Food Allergy Specific Immunotherapy (201871) (VLID)3845545

Published
2019
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39. Localization of Four Allergens in Artemisia Pollen by Immunofluorescent Antibodies

Author

Gao, Zhong-Shan, Fu, Wan-Yi, Zhao, Lan, Gao, Ling, Zhou, Jian-Ya, Gao, Bi-Yuan, Wu, Shandong, Versteeg, Serge A., Ferreira, Fatima, Gadermaier, Gabriele, van Ree, Ronald, Experimental Immunology, Ear, Nose and Throat, AII - Inflammatory diseases, APH - Global Health, and APH - Personalized Medicine

Subjects
otorhinolaryngologic diseases, food and beverages
Abstract

Background: Artemisia pollens have a high potential to induce allergic symptoms. Seven allergen components have been identified, but only Art v 7 has been localized in the pollen grain. This study aimed to localize the allergens in the pollen grains of 4 >italic/italic< spp. >bold<>italic/italic<>/bold< Pollen extracts from 2 Chinese >italic/italic< spp., >italic/italic< and >italic/italicitalic/italic< spp. (>italic/italicitalic/italic< >italic/italicitalic/italicitalic/italicbold<>italic/italic<>/bold< We obtained 2 specific mAbs against Art v 1, 1 against Art v 2, and 4 against Art v 3 homologs. The Art v 1 and Art v 3 homologs were mainly located on the pollen walls, and the Art v 7 homologous protein was localized intracellularly around nuclei. The location of the Art v 2 homologous protein varied across species, being intracellular around nuclei for >italic/italic< and >italic/italic< >italic/italicitalic/italicitalic/italic< and >italic/italic< >italic/italicbold<>italic/italic<>/bold< Four mugwort allergens were localized in the pollen, and the major Art v 1 and Art v 3 allergens were located mainly in the pollen wall.

Published
2019

41. Chemically modified peanut extract shows increased safety while maintaining immunogenicity

Author

van der Kleij, Hanneke P M, Warmenhoven, Hans J M, van Ree, Ronald, Versteeg, Serge A, Pieters, Raymond H H, Dreskin, Stephen C, Knulst, André C, Hoffen, Els van, Opstelten, Dirk Jan E, Koppelman, Stef J, Smit, Joost J, van der Kleij, Hanneke P M, Warmenhoven, Hans J M, van Ree, Ronald, Versteeg, Serge A, Pieters, Raymond H H, Dreskin, Stephen C, Knulst, André C, Hoffen, Els van, Opstelten, Dirk Jan E, Koppelman, Stef J, and Smit, Joost J

Abstract

BACKGROUND: Peanuts are most responsible for food-induced anaphylaxis in adults in developed countries. An effective and safe immunotherapy is urgently needed. The aim of this study was to investigate the immunogenicity, allergenicity, and immunotherapeutic efficacy of a well-characterized chemically modified peanut extract (MPE) adsorbed to Al(OH)3 .METHODS: Peanut extract (PE) was modified by reduction and alkylation. Using sera of peanut-allergic patients, competitive IgE-binding assays and mediator release assays were performed. The immunogenicity of MPE was evaluated by measuring activation of human PE-specific T-cell lines and the induction of PE-specific IgG in mice. The safety and efficacy of MPE adsorbed to Al(OH)3 was tested in two mouse models by measuring allergic manifestations upon peanut challenge in peanut-allergic mice.RESULTS: Compared to PE, the IgE-binding and capacity to induce allergic symptoms of MPE were lower in all patients. PE and MPE displayed similar immunogenicity in vivo and in vitro. In mice sensitized to PE, the threshold for anaphylaxis (drop in BT) upon subcutaneous challenge with PE was 0.01 mg, while at 0.3 mg MPE no allergic reaction occurred. Anaphylaxis was not observed when PE and MPE were fully adsorbed to Al(OH)3 . Both PE and MPE + Al(OH)3 showed to be efficacious in a model for immunotherapy.CONCLUSION: In our studies, an Al(OH)3 adsorbed MPE showed reduced allergenicity compared to unmodified PE, while the efficacy of immunotherapy is maintained. The preclinical data presented in this study supports further development of modified peanut allergens for IT.

Published
2019

42. Chemically modified peanut extract shows increased safety while maintaining immunogenicity

Author

van der Kleij, Hanneke P.M., Warmenhoven, Hans J.M., van Ree, Ronald, Versteeg, Serge A., Pieters, Raymond H.H., Dreskin, Stephen C., Knulst, André C., van Hoffen, Els, Opstelten, Dirk Jan E., Koppelman, Stef J., Smit, Joost J., van der Kleij, Hanneke P.M., Warmenhoven, Hans J.M., van Ree, Ronald, Versteeg, Serge A., Pieters, Raymond H.H., Dreskin, Stephen C., Knulst, André C., van Hoffen, Els, Opstelten, Dirk Jan E., Koppelman, Stef J., and Smit, Joost J.

Published
2019

43. Chemically modified peanut extract shows increased safety while maintaining immunogenicity

Author

UMC Utrecht, MS Dermatologie/Allergologie, Infection & Immunity, van der Kleij, Hanneke P.M., Warmenhoven, Hans J.M., van Ree, Ronald, Versteeg, Serge A., Pieters, Raymond H.H., Dreskin, Stephen C., Knulst, André C., van Hoffen, Els, Opstelten, Dirk Jan E., Koppelman, Stef J., Smit, Joost J., UMC Utrecht, MS Dermatologie/Allergologie, Infection & Immunity, van der Kleij, Hanneke P.M., Warmenhoven, Hans J.M., van Ree, Ronald, Versteeg, Serge A., Pieters, Raymond H.H., Dreskin, Stephen C., Knulst, André C., van Hoffen, Els, Opstelten, Dirk Jan E., Koppelman, Stef J., and Smit, Joost J.

Published
2019

44. Rational Design, Structure-Activity Relationship, and Immunogenicity of Hypoallergenic Pru p 3 Variants

Author

Eichhorn, Stephanie, Hörschläger, Angelika, Steiner, Markus, Laimer, Josef, Jensen, Bettina M, Versteeg, Serge A, Pablos, Isabel, Briza, Peter, Jongejan, Laurian, Rigby, Neil, Asturias, Juan A, Portolés, Antonio, Fernandez-Rivas, Montserrat, Papadopoulos, Nikolaos G, Mari, Adriano, Poulsen, Lars K, Lackner, Peter, van Ree, Ronald, Ferreira, Fatima, Gadermaier, Gabriele, Eichhorn, Stephanie, Hörschläger, Angelika, Steiner, Markus, Laimer, Josef, Jensen, Bettina M, Versteeg, Serge A, Pablos, Isabel, Briza, Peter, Jongejan, Laurian, Rigby, Neil, Asturias, Juan A, Portolés, Antonio, Fernandez-Rivas, Montserrat, Papadopoulos, Nikolaos G, Mari, Adriano, Poulsen, Lars K, Lackner, Peter, van Ree, Ronald, Ferreira, Fatima, and Gadermaier, Gabriele

Abstract

SCOPE: Allergies to lipid transfer proteins involve severe adverse reactions; thus, effective and sustainable therapies are desired. Previous attempts disrupting disulfide bonds failed to maintain immunogenicity; thus, the aim is to design novel hypoallergenic Pru p 3 variants and evaluate the applicability for treatment of peach allergy.METHODS AND RESULTS: Pru p 3 proline variant (PV) designed using in silico mutagenesis, cysteine variant (CV), and wild-type Pru p 3 (WT) are purified from Escherichia coli. Variants display homogenous and stable protein conformations with an altered secondary structure in circular dichroism. PV shows enhanced long-term storage capacities compared to CV similar to the highly stable WT. Using sera of 33 peach allergic patients, IgE-binding activity is reduced by 97% (PV) and 71% (CV) compared to WT. Both molecules show strong hypoallergenicity in Pru p 3 ImmunoCAP cross-inhibition and histamine release assays. Immunogenicity of PV is demonstrated with a phosphate-based adjuvant formulation in a mouse model.CONCLUSIONS: An in silico approach is used to generate a PV without targeting disulfide bonds, T cell epitopes, or previously reported IgE epitopes of Pru p 3. PV is strongly hypoallergenic while structurally stable and immunogenic, thus representing a promising candidate for peach allergen immunotherapy.

Published
2019

45. FAST: towards safe and effective subcutaneous immunotherapy of persistent life-threatening food allergies

Author

Zuidmeer-Jongejan Laurian, Fernandez-Rivas Montserrat, Poulsen Lars K, Neubauer Angela, Asturias Juan, Blom Lars, Boye Joyce, Bindslev-Jensen Carsten, Clausen Michael, Ferrara Rosa, Garosi Paula, Huber Hans, Jensen Bettina M, Koppelman Stef, Kowalski Marek L, Lewandowska-Polak Anna, Linhart Birgit, Maillere Bernard, Mari Adriano, Martinez Alberto, Mills Clare EN, Nicoletti Claudio, Opstelten Dirk-Jan, Papadopoulos Nikos G, Portoles Antonio, Rigby Neil, Scala Enrico, Schnoor Heidi J, Sigurdardottir Sigurveig T, Stavroulakis George, Stolz Frank, Swoboda Ines, Valenta Rudolf, van den Hout Rob, Versteeg Serge A, Witten Marianne, and van Ree Ronald

Subjects
FAST, Food allergy, Specific immunotherapy, Subcutaneous, Sublingual, Fish, Peach, Hypoallergens, Immunologic diseases. Allergy, RC581-607
Abstract

Abstract The FAST project (Food Allergy Specific Immunotherapy) aims at the development of safe and effective treatment of food allergies, targeting prevalent, persistent and severe allergy to fish and peach. Classical allergen-specific immunotherapy (SIT), using subcutaneous injections with aqueous food extracts may be effective but has proven to be accompanied by too many anaphylactic side-effects. FAST aims to develop a safe alternative by replacing food extracts with hypoallergenic recombinant major allergens as the active ingredients of SIT. Both severe fish and peach allergy are caused by a single major allergen, parvalbumin (Cyp c 1) and lipid transfer protein (Pru p 3), respectively. Two approaches are being evaluated for achieving hypoallergenicity, i.e. site-directed mutagenesis and chemical modification. The most promising hypoallergens will be produced under GMP conditions. After pre-clinical testing (toxicology testing and efficacy in mouse models), SCIT with alum-absorbed hypoallergens will be evaluated in phase I/IIa and IIb randomized double-blind placebo-controlled (DBPC) clinical trials, with the DBPC food challenge as primary read-out. To understand the underlying immune mechanisms in depth serological and cellular immune analyses will be performed, allowing identification of novel biomarkers for monitoring treatment efficacy. FAST aims at improving the quality of life of food allergic patients by providing a safe and effective treatment that will significantly lower their threshold for fish or peach intake, thereby decreasing their anxiety and dependence on rescue medication.

Published
2012
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46. Factors influencing adherence in a trial of early introduction of allergenic food

Author

Perkin, Michael R., primary, Bahnson, Henry T., additional, Logan, Kirsty, additional, Marrs, Tom, additional, Radulovic, Suzana, additional, Knibb, Rebecca, additional, Craven, Joanna, additional, Flohr, Carsten, additional, Mills, E.N., additional, Versteeg, Serge A., additional, van Ree, Ronald, additional, Lack, Gideon, additional, Young, Louise, additional, Offord, Victoria, additional, DeSousa, Mary, additional, Cullen, Jason, additional, Taylor, Katherine, additional, Tseng, Anna, additional, Raji, Bunmi, additional, Byrom, Sarah, additional, Regis, Gillian, additional, Bigwood, Charlie, additional, Stedman, Charlotte, additional, Tonner, Sharon, additional, Banks, Emily, additional, Kahnum, Yasmin, additional, Babic, Rachel, additional, Stockwell, Ben, additional, Thompson, Erin, additional, Wheatley, Lorna, additional, and Patkunam, Devi, additional

Published
2019
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47. Efficacy of the Enquiring About Tolerance (EAT) study among infants at high risk of developing food allergy

Author

Perkin, Michael R., primary, Logan, Kirsty, additional, Bahnson, Henry T., additional, Marrs, Tom, additional, Radulovic, Suzana, additional, Craven, Joanna, additional, Flohr, Carsten, additional, Mills, E.N., additional, Versteeg, Serge A., additional, van Ree, Ronald, additional, Lack, Gideon, additional, Young, Louise, additional, Offord, Victoria, additional, DeSousa, Mary, additional, Cullen, Jason, additional, Taylor, Katherine, additional, Tseng, Anna, additional, Raji, Bunmi, additional, Byrom, Sarah, additional, Regis, Gillian, additional, Bigwood, Charlie, additional, Stedman, Charlotte, additional, Tonner, Sharon, additional, Banks, Emily, additional, Kahnum, Yasmin, additional, Babic, Rachel, additional, Stockwell, Ben, additional, Thompson, Erin, additional, Wheatley, Lorna, additional, Patkunam, Devi, additional, Richards, Kerry, additional, Pietraszewicz, Ewa, additional, Stephens, Alick, additional, Sudra, Asha, additional, and Turcanu, Victor, additional

Published
2019
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48. Selection of Pru p 3 hypoallergenic peach and nectarine varieties

Author

Jin, Jing, primary, Gao, Ling, additional, Zhao, Lan, additional, Gao, Zhong‐shan, additional, Li, Xiong‐wei, additional, Xie, Han‐bing, additional, Ni, Jun‐bei, additional, Gan, Ke‐xin, additional, Wu, Shan‐dong, additional, Ye, Zheng‐wen, additional, Luo, Jun, additional, Cao, Ke, additional, Ma, Rui‐juan, additional, Chen, Miao‐jin, additional, Arús, Pere, additional, Versteeg, Serge A., additional, Wang, Hui‐ying, additional, Liu, Mei-ling, additional, Jia, Hui‐juan, additional, and Ree, Ronald, additional

Published
2019
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49. Rational Design, Structure–Activity Relationship, and Immunogenicity of Hypoallergenic Pru p 3 Variants

Author

Eichhorn, Stephanie, primary, Hörschläger, Angelika, additional, Steiner, Markus, additional, Laimer, Josef, additional, Jensen, Bettina M, additional, Versteeg, Serge A, additional, Pablos, Isabel, additional, Briza, Peter, additional, Jongejan, Laurian, additional, Rigby, Neil, additional, Asturias, Juan A, additional, Portolés, Antonio, additional, Fernandez‐Rivas, Montserrat, additional, Papadopoulos, Nikolaos G, additional, Mari, Adriano, additional, Poulsen, Lars K, additional, Lackner, Peter, additional, Ree, Ronald, additional, Ferreira, Fatima, additional, and Gadermaier, Gabriele, additional

Published
2019
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