Search

Your search keyword '"Veroutis, Dimitris"' showing total 30 results
Start Over Author "Veroutis, Dimitris"
30 results on '"Veroutis, Dimitris"'

1. A GATA2-CDC6 axis modulates androgen receptor blockade-induced senescence in prostate cancer

Author

Mourkioti, Ioanna, Polyzou, Aikaterini, Veroutis, Dimitris, Theocharous, George, Lagopati, Nefeli, Gentile, Emanuela, Stravokefalou, Vasiliki, Thanos, Dimitris-Foivos, Havaki, Sophia, Kletsas, Dimitris, Panaretakis, Theocharis, Logothetis, Christopher J., Stellas, Dimitris, Petty, Russell, Blandino, Giovanni, Papaspyropoulos, Angelos, and Gorgoulis, Vassilis G.

Published
2023
Full Text
View/download PDF

3. Cellular senescence in testicular cancer. Is there a correlation with the preoperative markers and the extent of the tumor? An experimental study

Author

Tatanis, Vasileios, primary, Veroutis, Dimitris, additional, Pantelis, Pavlos, additional, Theocharous, George, additional, Sarlanis, Helen, additional, Georgiou, Alexandros, additional, Peteinaris, Angelis, additional, Natsos, Anastasios, additional, Moulavasilis, Napoleon, additional, Kavantzas, Nikolaos, additional, Kotsinas, Athanassios, additional, and Adamakis, Ioannis, additional

Published
2024
Full Text
View/download PDF

4. One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Author

Magkouta, Sophia, primary, Veroutis, Dimitris, additional, Pousias, Athanasios, additional, Papaspyropoulos, Angelos, additional, Giannetti, Kety, additional, Pippa, Natassa, additional, Lougiakis, Nikolaos, additional, Kambas, Konstantinos, additional, Lagopati, Nefeli, additional, Polyzou, Aikaterini, additional, Georgiou, Maria, additional, Chountoulesi, Maria, additional, Pispas, Stergios, additional, Foutadakis, Spyros, additional, Kyrodimos, Efthymios, additional, Pouli, Nicole, additional, Marakos, Panagiotis, additional, Kotsinas, Athanassios, additional, Verginis, Panayotis, additional, Valakos, Dimitrios, additional, Vatsellas, Giannis, additional, Petty, Russell, additional, Thanos, Dimitris, additional, Demaria, Marco, additional, Evangelou, Konstantinos, additional, Di Micco, Raffaella, additional, and Gorgoulis, Vassilis G., additional

Published
2024
Full Text
View/download PDF

6. Cellular Senescence in Germ Cell Neoplasia In Situ (GCNIS) and Other Histological Types of Testicular Cancer.

Author

Tatanis, Vasileios, Veroutis, Dimitris, Pantelis, Pavlos, Theocharous, George, Sarlanis, Helen, Georgiou, Alexandros, Mulita, Francesk, Peteinaris, Angelis, Natsos, Anastasios, Moulavasilis, Napoleon, Kavantzas, Nikolaos, Kotsinas, Athanassios, and Adamakis, Ioannis

Subjects
CELLULAR aging, TESTICULAR cancer, GERM cells, TUMORS, PRECANCEROUS conditions, IMMUNOSENESCENCE, TESTIS surgery, ORCHIOPEXY, AGING
Abstract

Background and Objectives: The presence and contribution of senescent cells in premalignant lesions is well documented, but not in germ cell neoplasia in situ. The purpose of this study is to identify the presence of senescent cells in pre-malignant testicular conditions and in different histological types of testicular cancer. Materials and Methods: Thirty patients who underwent orchiectomy due to testicular tumors were included. Formalin-fixed paraffin-embedded (FFPE) testicular tissue for each patient was available. Sections from these specimens were examined by immunohistochemical analysis with the following markers: GL13 for cellular senescence, p21WAF1/Cip1 for cell cycle arrest, and Ki67 for cell proliferation. Results: Thirteen (43.3%) suffered from seminoma with a mean total proportion of GCNIS senescence of 20.81 ± 6.81%. In the group of embryonal testicular tumors, nine (30%) patients were included, with an average rate of 6.64 ± 5.42% of senescent cells in GCNIS. One (3.3%) patient suffered from chondrosarcoma in which 7.9% of GL13+ cells were detected in GCNIS. Four (13.4%) patients suffered from teratoma and three (10%) from yolk sac tumors, while GCNIS senescence was detected in a range of 4.43 ± 1.78% and 3.76 ± 1.37%, respectively. Conclusions: Cellular senescence was detected in both germ cell neoplasia in situ and testicular cancer, but was more prevalent within the premalignant lesions. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Pulsed Electromagnetic Fields (PEMFs) Trigger Cell Death and Senescence in Cancer Cells

Author

Pantelis, Pavlos, primary, Theocharous, Giorgos, additional, Veroutis, Dimitris, additional, Vagena, Ioanna-Aglaia, additional, Polyzou, Aikaterini, additional, Thanos, Dimitris-Foivos, additional, Kyrodimos, Efthymios, additional, Kotsinas, Athanassios, additional, Evangelou, Konstantinos, additional, Lagopati, Nefeli, additional, Gorgoulis, Vassilis G., additional, and Kotopoulos, Nicholas, additional

Published
2024
Full Text
View/download PDF

8. Senescent cells in giant cell arteritis display an inflammatory phenotype participating in tissue injury via IL-6-dependent pathways.

Author

Veroutis, Dimitris, Argyropoulou, Ourania D., Goules, Andreas V., Kambas, Konstantinos, Palamidas, Dimitris Anastasios, Evangelou, Konstantinos, Havaki, Sophia, Polyzou, Aikaterini, Valakos, Dimitrios, Xingi, Evangelia, Karatza, Elli, Boki, Kyriaki A., Cavazza, Alberto, Kittas, Christos, Thanos, Dimitris, Ricordi, Caterina, Marvisi, Chiara, Muratore, Francesco, Galli, Elena, and Croci, Stefania

Published
2024
Full Text
View/download PDF

9. Senescent cells in giant cell arteritis display an inflammatory phenotype participating in tissue injury via IL-6-dependent pathways

Author

Veroutis, Dimitris, primary, Argyropoulou, Ourania D, additional, Goules, Andreas V, additional, Kambas, Konstantinos, additional, Palamidas, Dimitris Anastasios, additional, Evangelou, Konstantinos, additional, Havaki, Sophia, additional, Polyzou, Aikaterini, additional, Valakos, Dimitrios, additional, Xingi, Evangelia, additional, Karatza, Elli, additional, Boki, Kyriaki A, additional, Cavazza, Alberto, additional, Kittas, Christos, additional, Thanos, Dimitris, additional, Ricordi, Caterina, additional, Marvisi, Chiara, additional, Muratore, Francesco, additional, Galli, Elena, additional, Croci, Stefania, additional, Salvarani, Carlo, additional, Gorgoulis, Vassilis G, additional, and Tzioufas, Athanasios G, additional

Published
2023
Full Text
View/download PDF

10. A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells

Author

Magkouta, Sophia, primary, Veroutis, Dimitris, additional, Pousias, Athanasios, additional, Papaspyropoulos, Angelos, additional, Pippa, Natassa, additional, Lougiakis, Nikolaos, additional, Kambas, Konstantinos, additional, Lagopati, Nefeli, additional, Polyzou, Aikaterini, additional, Georgiou, Maria, additional, Chountoulesi, Maria, additional, Pispas, Stergios, additional, Foutadakis, Spyros, additional, Pouli, Nicole, additional, Marakos, Panagiotis, additional, Kotsinas, Athanassios, additional, Verginis, Panayotis, additional, Valakos, Dimitrios, additional, Mizi, Athanasia, additional, Papantonis, Argyris, additional, Vatsellas, Giannis, additional, Galanos, Panagiotis, additional, Bartek, Jiri, additional, Petty, Russell, additional, Serrano, Manuel, additional, Thanos, Dimitris, additional, Roussos, Charis, additional, Demaria, Marco, additional, Evangelou, Konstantinos, additional, and Gorgoulis, Vassilis G., additional

Published
2023
Full Text
View/download PDF

12. Senescent cells in Giant Cell Arteritis have inflammatory phenotype participating in tissue injury via IL-6 dependent pathways

Author

Veroutis, Dimitris, primary, Argyropoulou, Ourania D, additional, Goules, Andreas V, additional, Kambas, Konstantinos, additional, Palamidas, Dimitris Anastasios, additional, Evangelou, Konstantinos, additional, Havaki, Sophia, additional, Polyzou, Aikaterini, additional, Xingi, Evangelia, additional, Karatza, Elli, additional, Kyriaki, Boki A, additional, Cavazza, Alberto, additional, Kittas, Christos, additional, Thanos, Dimitris, additional, Ricordi, Caterina, additional, Marvisi, Chiara, additional, Muratore, Francesco, additional, Galli, Elena, additional, Croci, Stefania, additional, Salvarani, Carlo, additional, Gorgoulis, Vassilis G, additional, and Tzioufas, Athanasios G, additional

Published
2023
Full Text
View/download PDF

13. The Dual Role of Oxidative-Stress-Induced Autophagy in Cellular Senescence: Comprehension and Therapeutic Approaches

Author

Pantelis, Pavlos, primary, Theocharous, George, additional, Lagopati, Nefeli, additional, Veroutis, Dimitris, additional, Thanos, Dimitris-Foivos, additional, Lampoglou, Giasemi-Panagiota, additional, Pippa, Natassa, additional, Gatou, Maria-Anna, additional, Tremi, Ioanna, additional, Papaspyropoulos, Angelos, additional, Kyrodimos, Efthymios, additional, Pavlatou, Evangelia A., additional, Gazouli, Maria, additional, Evangelou, Konstantinos, additional, and Gorgoulis, Vassilis G., additional

Published
2023
Full Text
View/download PDF

14. A tumour-promoting senescent secretome triggered by platinum chemotherapy exploits a targetable TGFβR1/Akt-mTOR axis in lung cancer

Author

González-Gualda, Estela, primary, Macias, David, additional, Morsli, Samir, additional, Martín, José Ezequiel, additional, Ou, Hui-Ling, additional, Denholm, Mary, additional, Olan, Ioana, additional, Hoffmann, Reuben, additional, Dane, Mark, additional, Veroutis, Dimitris, additional, Medrano, Guillermo, additional, Mulero, Francisca, additional, Martins, Carla P., additional, Barbacid, Mariano, additional, Gorgoulis, Vassilis, additional, Korkola, James E., additional, Rassl, Doris M., additional, Doherty, Gary J., additional, Rintoul, Robert C., additional, Narita, Masashi, additional, and Muñoz-Espín, Daniel, additional

Published
2022
Full Text
View/download PDF

15. Increase of HO-1 Expression in Critically Ill COVID-19 Patients Is Associated with Poor Prognosis and Outcome

Author

Detsika, Maria G., primary, Nikitopoulou, Ioanna, additional, Veroutis, Dimitris, additional, Vassiliou, Alice G., additional, Jahaj, Edison, additional, Tsipilis, Stamatis, additional, Athanassiou, Nikolaos, additional, Gakiopoulou, Hariklia, additional, Gorgoulis, Vassilis G., additional, Dimopoulou, Ioanna, additional, Orfanos, Stylianos E., additional, and Kotanidou, Anastasia, additional

Published
2022
Full Text
View/download PDF

16. Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: possible implications for viral mutagenesis

Author

Evangelou, Konstantinos, Veroutis, Dimitris, Paschalaki, Koralia, Foukas, Periklis G., Lagopati, Nefeli, Dimitriou, Marios, Papaspyropoulos, Angelos, Konda, Bindu, Hazapis, Orsalia, Polyzou, Aikaterini, Havaki, Sophia, Kotsinas, Athanassios, Kittas, Christos, Tzioufas, Athanasios G., De Leval, Laurence, Vassilakos, Demetris, Tsiodras, Sotirios, Stripp, Barry R., Papantonis, Argyris, Blandino, Giovanni, Karakasiliotis, Ioannis, Barnes, Peter J., Gorgoulis, Vassilis G., Evangelou, Konstantinos, Veroutis, Dimitris, Paschalaki, Koralia, Foukas, Periklis G., Lagopati, Nefeli, Dimitriou, Marios, Papaspyropoulos, Angelos, Konda, Bindu, Hazapis, Orsalia, Polyzou, Aikaterini, Havaki, Sophia, Kotsinas, Athanassios, Kittas, Christos, Tzioufas, Athanasios G., De Leval, Laurence, Vassilakos, Demetris, Tsiodras, Sotirios, Stripp, Barry R., Papantonis, Argyris, Blandino, Giovanni, Karakasiliotis, Ioannis, Barnes, Peter J., and Gorgoulis, Vassilis G.

Abstract

Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of the respiratory system can progress to a multisystemic disease with aberrant inflammatory response. Cellular senescence promotes chronic inflammation, named senescence-associated secretory phenotype (SASP). We investigated whether coronavirus disease 2019 (COVID-19) is associated with cellular senescence and SASP. Methods Autopsy lung tissue samples from 11 COVID-19 patients and 43 age-matched non-COVID-19 controls with similar comorbidities were analysed by immunohistochemistry for SARS-CoV-2, markers of senescence and key SASP cytokines. Virally induced senescence was functionally recapitulated in vitro, by infecting epithelial Vero-E6 cells and a three-dimensional alveosphere system of alveolar type 2 (AT2) cells with SARS-CoV-2 strains isolated from COVID-19 patients. Results SARS-CoV-2 was detected by immunocytochemistry and electron microscopy predominantly in AT2 cells. Infected AT2 cells expressed angiotensin-converting enzyme 2 and exhibited increased senescence (p16(INK4A) and SenTraGor positivity) and interleukin (IL)-1 beta and IL-6 expression. In vitro, infection of Vero-E6 cells with SARS-CoV-2 induced senescence (SenTraGor), DNA damage (gamma-H2AX) and increased cytokine (IL-1 beta, IL-6, CXCL8) and apolipoprotein B mRNA-editing (APOBEC) enzyme expression. Next-generation sequencing analysis of progenies obtained from infected/senescent Vero-E6 cells demonstrated APOBEC-mediated SARS-CoV-2 mutations. Dissemination of the SARS-CoV-2-infection and senescence was confirmed in extrapulmonary sites (kidney and liver) of a COVID-19 patient. Conclusions We demonstrate that in severe COVID-19, AT2 cells infected by SARS-CoV-2 exhibit senescence and a proinflammatory phenotype. In vitro, SARS-CoV-2 infection induces senescence and inflammation. Importantly, infected senescent cells may act as a source of SARS-CoV-2 mutagenesis mediated by APOBEC enzymes. Therefore, SARS-CoV-2

Published
2022

17. Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: possible implications for viral mutagenesis

Author

Evangelou, Konstantinos, primary, Veroutis, Dimitris, additional, Paschalaki, Koralia, additional, Foukas, Periklis G., additional, Lagopati, Nefeli, additional, Dimitriou, Marios, additional, Papaspyropoulos, Angelos, additional, Konda, Bindu, additional, Hazapis, Orsalia, additional, Polyzou, Aikaterini, additional, Havaki, Sophia, additional, Kotsinas, Athanassios, additional, Kittas, Christos, additional, Tzioufas, Athanasios G., additional, de Leval, Laurence, additional, Vassilakos, Demetris, additional, Tsiodras, Sotirios, additional, Stripp, Barry R., additional, Papantonis, Argyris, additional, Blandino, Giovanni, additional, Karakasiliotis, Ioannis, additional, Barnes, Peter J., additional, and Gorgoulis, Vassilis G., additional

Published
2022
Full Text
View/download PDF

21. Evaluation of senescent cells in intervertebral discs by lipofuscin staining

Author

Veroutis, Dimitris Kouroumalis, Anastasios Lagopati, Nefeli and Polyzou, Aikaterini Chamilos, Christos Papadodima, Stavroula and Evangelou, Konstantinos Gorgoulis, Vassilis G. Kletsas, Dimitris

Abstract

Intervertebral disc (IVD) degeneration is considered an important contributor of low back pain, a major age related disease. Interestingly, an unprecedented high number of senescent cells has been reported in aged and degenerated IVDs, most probably affecting tissue homeostasis. In previous studies classical markers of cellular senescence have been used, such as SA-beta-gal staining or p16(Ink4a) expression. Aim of the presented study was a reevaluation of the number of senescent IVD cells by using a newly established staining procedure for lipofuscin, based on a Sudan Black-B analogue (GL13), which can be used in fresh, as well as in fixed and embedded tissues. In cultures of senescent rat and human IVD cells both SA-beta-gal and GL13 gave similar percentages of senescent cells. Similarly, in fresh tissues from old rats the ratios of senescent cells were high with both detection procedures. Finally, in formalin-fixed and paraffin-embedded tissues from humans, a significant increased number of GL13-positive cells was found in herniated tissues, as compared to apparently normal ones, while similar numbers of p16(Ink4a)-positive cells were observed. These data confirm the significantly enhanced number of senescent cells in aged and degenerated IVDs, most probably contributing to the degeneration of this tissue.

Published
2021

22. Biological Effect of Silver-modified Nanostructured Titanium Dioxide in Cancer

Author

Lagopati, Nefeli, Kotsinas, Athanassios, Veroutis, Dimitris, Evangelou, Konstantinos, Papaspyropoulos, Angelos, Arfanis, Michalis, Falaras, Polycarpos, Kitsiou, Paraskevi V., Pateras, Ioannis, Bergonzini, Anna, Frisan, Teresa, Kyriazis, Spyridon, Tsoukleris, Dimitrios S., Tsilibary, Effie-Photini C, Gazouli, Maria, Pavlatou, Evangelia A., Gorgoulis, Vassilis G., Lagopati, Nefeli, Kotsinas, Athanassios, Veroutis, Dimitris, Evangelou, Konstantinos, Papaspyropoulos, Angelos, Arfanis, Michalis, Falaras, Polycarpos, Kitsiou, Paraskevi V., Pateras, Ioannis, Bergonzini, Anna, Frisan, Teresa, Kyriazis, Spyridon, Tsoukleris, Dimitrios S., Tsilibary, Effie-Photini C, Gazouli, Maria, Pavlatou, Evangelia A., and Gorgoulis, Vassilis G.

Abstract

BACKGROUND/AIM: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2 NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2 optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2 NPs) with anticancer potential. MATERIALS AND METHODS: Ag/TiO2 NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDA-MB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. RESULTS AND CONCLUSION: We show that photo-activated Ag/TiO2 NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.

Published
2021
Full Text
View/download PDF

24. Carotid Disease and Ageing: A Literature Review on the Pathogenesis of Vascular Senescence in Older Subjects

Author

Sertedaki, Eleni Veroutis, Dimitris Zagouri, Flora Galyfos, George Filis, Konstadinos Papalambros, Alexandros Aggeli, Konstantina Tsioli, Panagiota Charalambous, George Zografos, George Sigala, Fragiska

Abstract

Aging is a natural process that affects all systems of the human organism, leading to its inability to adapt to environmental changes. Advancing age has been correlated with various pathological conditions, especially cardiovascular and cerebrovascular diseases. Carotid artery (CA) is mainly affected by age-induced functional and morphological alterations causing atheromatous disease. The evolvement of biomedical sciences has allowed the elucidation of many aspects of this condition. Symptomatic carotid disease (CD) derives from critical luminar stenosis or eruption of an atheromatous plaque due to structural modifications of the vessels, such as carotid intima-media thickening. At a histologic level, the aforementioned changes are mediated by elastin fragmentation, collagen deposition, immune cell infiltration, and accumulation of cytokines and vasoconstrictors. Underlying mechanisms include chronic inflammation and oxidative stress, dysregulation of cellular homeostatic systems, and senescence. Thus, there is an imbalance in components of the vessel wall, which fails to counteract exterior stress stimuli. Consequently, arterial relaxation is impaired and atherosclerotic lesions progress. This is a review of current evidence regarding the relationship of aging with vascular senescence and CD. A deeper understanding of these mechanisms can contribute to the production of efficient prevention methods and targeted therapeutic strategies.

Published
2020

25. Carotid Disease and Ageing: A Literature Review on the Pathogenesis of Vascular Senescence in Older Subjects

Author

Sertedaki, Eleni, Veroutis, Dimitris, Zagouri, Flora, Galyfos, George, Filis, Konstadinos, Papalambros, Alexandros, Aggeli, Konstantina, Tsioli, Panagiota, Charalambous, George, Zografos, George, and Sigala, Fragiska

Subjects
Article Subject
Abstract

Aging is a natural process that affects all systems of the human organism, leading to its inability to adapt to environmental changes. Advancing age has been correlated with various pathological conditions, especially cardiovascular and cerebrovascular diseases. Carotid artery (CA) is mainly affected by age-induced functional and morphological alterations causing atheromatous disease. The evolvement of biomedical sciences has allowed the elucidation of many aspects of this condition. Symptomatic carotid disease (CD) derives from critical luminar stenosis or eruption of an atheromatous plaque due to structural modifications of the vessels, such as carotid intima-media thickening. At a histologic level, the aforementioned changes are mediated by elastin fragmentation, collagen deposition, immune cell infiltration, and accumulation of cytokines and vasoconstrictors. Underlying mechanisms include chronic inflammation and oxidative stress, dysregulation of cellular homeostatic systems, and senescence. Thus, there is an imbalance in components of the vessel wall, which fails to counteract exterior stress stimuli. Consequently, arterial relaxation is impaired and atherosclerotic lesions progress. This is a review of current evidence regarding the relationship of aging with vascular senescence and CD. A deeper understanding of these mechanisms can contribute to the production of efficient prevention methods and targeted therapeutic strategies.

Published
2020
Full Text
View/download PDF

27. SARS-CoV-2 infects lung epithelial cells and induces senescence and an inflammatory response in patients with severe COVID-19

Author

Evangelou, Konstantinos, primary, Veroutis, Dimitris, additional, Foukas, Periklis G, additional, Paschalaki, Koralia, additional, Lagopati, Nefeli, additional, Dimitriou, Marios, additional, Papaspyropoulos, Angelos, additional, Hazapis, Orsalia, additional, Polyzou, Aikaterini, additional, Havaki, Sophia, additional, Kotsinas, Athanassios, additional, Kittas, Christos, additional, Tzioufas, Athanasios G, additional, de Leval, Laurence, additional, Vassilakos, Demetris, additional, Tsiodras, Sotirios, additional, Karakasiliotis, Ioannis, additional, Barnes, Peter J, additional, and Gorgoulis, Vassilis, additional

Published
2021
Full Text
View/download PDF

28. Biological Effect of Silver-modified Nanostructured Titanium Dioxide in Cancer

Author

LAGOPATI, NEFELI, primary, KOTSINAS, ATHANASSIOS, additional, VEROUTIS, DIMITRIS, additional, EVANGELOU, KONSTANTINOS, additional, PAPASPYROPOULOS, ANGELOS, additional, ARFANIS, MICHALIS, additional, FALARAS, POLYCARPOS, additional, KITSIOU, PARASKEVI V., additional, PATERAS, IOANNIS, additional, BERGONZINI, ANNA, additional, FRISAN, TERESA, additional, KYRIAZIS, SPYRIDON, additional, TSOUKLERIS, DIMITRIOS S., additional, TSILIBARY, EFFIE-PHOTINI C., additional, GAZOULI, MARIA, additional, PAVLATOU, EVANGELIA A., additional, and GORGOULIS, VASSILIS G., additional

Published
2021
Full Text
View/download PDF

30. Biological Effect of Silver-modified Nanostructured Titanium Dioxide in Cancer.

Author

Lagopati N, Kotsinas A, Veroutis D, Evangelou K, Papaspyropoulos A, Arfanis M, Falaras P, Kitsiou PV, Pateras I, Bergonzini A, Frisan T, Kyriazis S, Tsoukleris DS, Tsilibary EC, Gazouli M, Pavlatou EA, and Gorgoulis VG

Subjects
Humans, Titanium pharmacology, Nanomedicine methods, Nanostructures chemistry, Neoplasms chemically induced, Silver chemistry, Titanium therapeutic use
Abstract

Background/aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO 2 NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO 2 optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO 2 NPs) with anticancer potential., Materials and Methods: Ag/TiO 2 NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDA-MB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection., Results and Conclusion: We show that photo-activated Ag/TiO 2 NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions., (Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results