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376 results on '"Vernos, Isabelle"'

3. Autosomal recessive variants in TUBGCP2 alter the γ-tubulin ring complex leading to neurodevelopmental disease

Author

Gungor, Serdal, Oktay, Yavuz, Hiz, Semra, Aranguren-Ibáñez, Álvaro, Kalafatcilar, Ipek, Yaramis, Ahmet, Karaca, Ezgi, Yis, Uluc, Sonmezler, Ece, Ekinci, Burcu, Aslan, Mahmut, Yilmaz, Elmasnur, Özgör, Bilge, Balaraju, Sunitha, Szabo, Nora, Laurie, Steven, Beltran, Sergi, MacArthur, Daniel G., Hathazi, Denisa, Töpf, Ana, Roos, Andreas, Lochmuller, Hanns, Vernos, Isabelle, and Horvath, Rita

Published
2021
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5. Views from a continent in flux

Author

Moedas, Carlos, Vernos, Isabelle, Kuster, Stephan, Nowotny, Helga, Saltelli, Andrea, Mungiu-Pippidi, Alina, and Wouter Vasbinder, Jan

Subjects
Influence, Beliefs, opinions and attitudes, Forecasts and trends, Market trend/market analysis, Research scientists -- Beliefs, opinions and attitudes, Trust (Psychology) -- Forecasts and trends -- Influence, Love -- Forecasts and trends -- Influence, Research funding -- Forecasts and trends -- Influence, Science -- Forecasts and trends
Abstract

Author(s): Carlos Moedas, Isabelle Vernos, Stephan Kuster, Helga Nowotny, Andrea Saltelli, Alina Mungiu-Pippidi, Jan Wouter Vasbinder, Daniel R. Brooks, Patrick Cunningham Author Affiliations: Views from a continent in flux Hungarian [...], Nature asked nine leading Europeans to pick their top priority for science at this pivotal point. Love, money, and trust got most votes. Nature asked nine leading Europeans to pick their top priority for science at this pivotal point. Love, money, and trust got most votes.

Published
2019
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6. A giant leap for womankind

Author

Valantine, Hannah, Travis, Elizabeth, El-Adhami, Wafa, Vernos, Isabelle, Mosqueda, Laura, Wayne, Elizabeth, Kearns-Zimmerman, Fiona, Bonefont, Lauren, Visweswariah, Sandhya S., Akande-Sholabi, Wuraola, and Polka, Jessica

Published
2019
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12. Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis.

Author

Verrillo, Giulia, Maria Obeid, Anna, Genco, Alexia, Scrofani, Jacopo, Orange, François, Hanache, Sarah, Mignon, Julien, Leyder, Tanguy, Michaux, Catherine, Kempeneers, Céline, Bricmont, Noeömie, Herkenne, Stephanie, Vernos, Isabelle, Martin, Maud, and Mottet, Denis

Subjects
CHROMATIN-remodeling complexes, CHROMOSOME segregation, MITOSIS, KINETOCHORE, CHROMATIN, GENE expression, CELL death, SLEEP spindles
Abstract

DPF3, along with other subunits, is a well-known component of the BAF chromatin remodeling complex, which plays a key role in regulating chromatin remodeling activity and gene expression. Here, we elucidated a non-canonical localization and role for DPF3. We showed that DPF3 dynamically localizes to the centriolar satellites in interphase and to the centrosome, spindle midzone and bridging fiber area, and midbodies during mitosis. Loss of DPF3 causes kinetochore fiber instability, unstable kinetochore-microtubule attachment and defects in chromosome alignment, resulting in altered mitotic progression, cell death and genomic instability. In addition, we also demonstrated that DPF3 localizes to centriolar satellites at the base of primary cilia and is required for ciliogenesis by regulating axoneme extension. Taken together, these findings uncover a moonlighting dual function for DPF3 during mitosis and ciliogenesis. [ABSTRACT FROM AUTHOR]

Published
2024
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31. A kinesin-like motor inhibits microtubule dynamic instability

Author

Bringman, Henrik, Skiniotis, Georgios, Spilker, Annina, Kandels-Lewis, Stefanie, Vernos, Isabelle, and Surrey, Thomas

Subjects
Physiological aspects, Molecular dynamics -- Physiological aspects, Microtubules -- Physiological aspects
Abstract

The activities of a variety of molecular motors are required to spatially organize microtubules and chromosomes in the mitotic spindle (1, 2). Some motors contribute to spindle pole focusing (3, [...], The motility of molecular motors and the dynamic instability of microtubules are key dynamic processes for mitotic spindle assembly and function. We report here that one of the mitotic kinesins that localizes to chromosomes, Xklp1 from Xenopus laevis, could inhibit microtubule growth and shrinkage. This effect appeared to be mediated by a structural change in the microtubule lattice. We also found that Xklp1 could act as a fast, nonprocessive, plus end--directed molecular motor. The integration of the two properties, motility and inhibition of microtubule dynamics, in one molecule emphasizes the versatile properties of kinesin family members.

Published
2004

35. Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity

Author

Gruss, Oliver J., Carazo-Salas, Rafael E., Schatz, Christoph A., Guarguaglini, Giulia, Kast, Jurgen, Wilm, Matthias, Le Bot, Nathalie, Vernos, Isabelle, Karsenti, Eric, and Mattaj, Iain W.

Subjects
Spindle (Cell division) -- Physiological aspects, Cell division -- Physiological aspects, G proteins -- Physiological aspects, Microtubules -- Physiological aspects, Biological sciences
Abstract

Chromosomal spindle formation during the M phase of mitosis by Ran.GTP is mediated by a microtubule-associated protein, TPX2, which in turn is regulated by nuclear import factor, importin alpha.

Published
2001

37. Xkid, a chromokinesin required for chromosome alignment on the metaphase plate

Author

Antonio, Celia, Ferby, Ingvar, Wilhelm. Heike, Jones, Margaret, Karsenti, Eric, Nebreda, Angel R., and Vernos, Isabelle

Subjects
Cell division -- Physiological aspects, Chromosome banding -- Analysis, Kinesin -- Physiological aspects, Mitosis -- Physiological aspects, Biological sciences
Abstract

Results demonstrate the role of a kinesin-like protein in the equatorial alignment of the chromosomes and its maintenance during metaphase of the cell division. Data indicate that the force exerted by the protein aligns kinetochores and chromosomes on a narrow equatorial plate.

Published
2000

43. Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis

Author

Boleti, Haralabia, Karsenti, Eric, and Vernos, Isabelle

Subjects
Kinesin -- Research, Microtubules -- Research, Mitosis -- Research, Spindle (Cell division) -- Research, Proteins -- Structure, Biological sciences
Abstract

A protein with a structure similar to kinesin was observed in Xenopus cells which directs the formation of mitotic spindles and the segregation of chromosomes during mitosis. The protein Xklp2 has a structure composed of a globular domain at its N-terminal and an alpha-helical tail that ends with the c-terminal. Mutations of both these ends caused a failure in chromosome separation during mitosis and the formation of abnormal spindles.

Published
1996

45. XKlp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning

Author

Vernos, Isabelle, Raats, Jos, Hirano, Tatsuya, Heasman, Janet, Karsenti, Eric, and Wylie, Chris

Subjects
Xenopus -- Research, Proteins -- Analysis, Mitosis -- Analysis, Spindle (Cell division) -- Observations, Biological sciences
Abstract

A Xenopus kinesin-like protein, XKlp1, is required for the formation of spindles and determines the position of chromosomes. The XKlp1 is in the nucleus at the interphase and in the chromosomes during the process of mitosis. At the anaphase, it develops proteins in the spindle interzone which later on arrange in the midbody at telophase. The XKlp1 amino terminus has a motor domain, and the tail may have a zinc finger-like area.

Published
1995

46. Multiple kinesin-like transcripts in Xenopus oocytes

Author

Vernos, Isabelle, Heasman, Janet, and Wylie, Chris

Subjects
Oocytes -- Genetic aspects, Genetic transcription -- Research, Xenopus -- Genetic aspects, Kinesin -- Genetic aspects, Proteins -- Research, Biological sciences
Abstract

A study was conducted on the extent of expression and the characteristics of various kinesin-like proteins (Klps) in Xenophus oocytes. Four new Klp mRNAs were identified and sequenced from oocytes through polymerase chain reaction, one of which appeared to be the counterpart of Drosophila Klp4. Xenopus oocyte Klps were found to assume different expression patterns during embryogenesis. It was suggested that during, early development, at least four Klps can coexist in a single cell and can be differentially regulated.

Published
1993

50. The C-terminal domain of TPX2 is made of alpha-helical tandem repeats

Author

Sanchez-Pulido, Luis, Perez, Laurent, Kuhn, Steffen, Vernos, Isabelle, Andrade-Navarro, Miguel A., Sanchez-Pulido, Luis, Perez, Laurent, Kuhn, Steffen, Vernos, Isabelle, and Andrade-Navarro, Miguel A.

Abstract

Background: TPX2 (Targeting Protein for Xklp2) is essential for spindle assembly, activation of the mitotic kinase Aurora A and for triggering microtubule nucleation. Homologs of TPX2 in Chordata and plants were previously identified. Currently, proteins of the TPX2 family have little structural information and only small parts are covered by defined protein domains. Methods: We have used computational sequence analyses and structural predictions of proteins of the TPX2 family, supported with Circular Dichroism (CD) measurements. Results: Here, we report our finding that the C-terminal domain of TPX2, which is responsible of its microtubule nucleation capacity and is conserved in all members of the family, is actually formed by tandem repeats, covering well above 2/3 of the protein. We propose that this region forms a flexible solenoid involved in protein-protein interactions. Structural prediction and molecular modeling, combined with Circular Dichroism (CD) measurements reveal a predominant alpha-helical content. Furthermore, we identify full length homologs in fungi and shorter homologs with a different domain organization in diptera (including a paralogous expansion in Drosophila). Conclusions: Our results, represent the first computational and biophysical analysis of the TPX2 proteins family and help understand the structure and evolution of this conserved protein family to direct future structural studies.

Published
2019

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