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46 results on '"Verkhovskii, Roman A."'

1. Cytotoxicity of various types of coated upconversion nanoparticles. Overview

Author

Verkhovskii, Roman A., Anisimov, Roman A., Lomova, Maria V., Tuchina, Daria K., Lazareva, Ekaterina Nikolaevna, Doronkina, Anna A., Mylnikov, Artyom M., Navolokin, Nikita Aleksandrovich, Kochubey, Vyacheslav Ivanovich, and Yanina, Irina Iur'evna

Subjects
cytotoxicity, upconversion particles, shell, concentration, dose, Physics, QC1-999
Abstract

Background and Objectives: The object of the study was the cytotoxicity of various types of coated upconversion nanoparticles. The aim is to overview the literature on the cytotoxicity of various types of upconversion nanoparticles without/with coating and to search for their maximum permissible concentration when applied to cell. Materials and Methods: The approach used has been the analysis of recent publications on the topic. Results: Upconversion nanoparticles are promising for fluorescence imaging and cancer therapy. Nanoparticles with additional shells or functionalized by surface coating with targeted or photoactive molecules are considered. The toxicological effect of nanoparticles on living organisms is of decisive importance when they are used in therapy or diagnostics. The “dark” cytotoxicity of particles is considered. The cytotoxicity of particles depends on the total number of nanoparticles that have penetrated into the cell. Conclusion: Based on the analysis of a large number of publications, it can be concluded that nanoparticles coated with silicon dioxide (SiO2) are characterized by the least cytotoxic effect, which opens up prospects for the use of this type of nanoparticles in medical practice.

Published
2022
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8. Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate.

Author

Saveleva, Mariia S., Verkhovskii, Roman A., Demina, Polina A., Surkov, Yury I., Anisimov, Roman A., Prikhozhdenko, Ekaterina S., Pidenko, Pavel S., Serebryakova, Isabella A., Zaytsev, Sergey M., Tuchin, Valery V., and Svenskaya, Yulia I.

Abstract

Inflammatory dermatoses represent a global problem with increasing prevalence and recurrence among the world population. Topical glucocorticoids (GCs) are the most commonly used anti-inflammatory drugs in dermatology due to a wide range of their therapeutic actions, which, however, have numerous local and systemic side effects. Hence, there is a growing need to create new delivery systems for GCs, ensuring the drug localization in the pathological site, thus increasing the effectiveness of therapy and lowering the risk of side effects. Here, we propose a novel topical particulate formulation for the GC clobetasol propionate (CP), based on the use of porous calcium carbonate (CaCO3) carriers in the vaterite crystalline form. The designed carriers contain a substantially higher CP amount than conventional dosage forms used in clinics (4.5% w/w vs. 0.05% w/w) and displayed a good biocompatibility and effective cellular uptake when studied in fibroblasts in vitro. Hair follicles represent an important reservoir for the GC accumulation in skin and house the targets for its action. In this study, we demonstrated successful delivery of the CP-loaded carriers (CP–CaCO3) into the hair follicles of rats in vivo using optical coherent tomography (OCT). Importantly, the OCT monitoring revealed the gradual intrafollicular degradation of the carriers within 168 h with the most abundant follicle filling occurring within the first 48 h. Biodegradability makes the proposed system especially promising when searching for new CP formulations with improved safety and release profile. Our findings evidenced the great potential of the CaCO3 carriers in improving the dermal bioavailability of this poorly water-soluble GC. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Effect of pulsed laser parameters on photoacoustic flow cytometry efficiency in vitro and in vivo

Author

Grishin, Oleg V., primary, Shushunova, Natalia A., additional, Bratashov, Daniil N., additional, Prikhozhdenko, Ekaterina S., additional, Verkhovskii, Roman A., additional, Kozlova, Anastasiia A., additional, Abdurashitov, Arkady S., additional, Sindeeva, Olga A., additional, Karavaev, Anatoly S., additional, Kulminskiy, Danil D., additional, Shashkov, Evgeny V., additional, Inozemtseva, Olga A., additional, and Tuchin, Valery V., additional

Published
2023
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10. Correction: Verkhovskii et al. The Influence of Magnetic Composite Capsule Structure and Size on Their Trapping Efficiency in the Flow. Molecules 2022, 27, 6073

Author

Verkhovskii, Roman, primary, Ermakov, Alexey, additional, Grishin, Oleg, additional, Makarkin, Mikhail A., additional, Kozhevnikov, Ilya, additional, Makhortov, Mikhail, additional, Kozlova, Anastasiia, additional, Salem, Samia, additional, Tuchin, Valery, additional, and Bratashov, Daniil, additional

Published
2023
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12. Cytotoxicity, Dermal Toxicity, and In Vivo Antifungal Effect of Griseofulvin-Loaded Vaterite Carriers Administered via Sonophoresis

Author

Verkhovskii, Roman A., primary, Kozlova, Anastasiia A., additional, Lengert, Ekaterina V., additional, Saveleva, Mariia S., additional, Makarkin, Mikhail A., additional, Mylnikov, Artem M., additional, Navolokin, Nikita A., additional, Bucharskaya, Alla B., additional, Terentyuk, Georgy S., additional, Bosak, Ilya A., additional, Vasilyeva, Natalya V., additional, and Svenskaya, Yulia S., additional

Published
2023
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14. Macrophage In Vitro and In Vivo Tracking via Anchored Microcapsules

Author

Sapach, Anastasiia Yu., primary, Sindeeva, Olga A., additional, Nesterchuk, Mikhail V., additional, Tsitrina, Alexandra A., additional, Mayorova, Oksana A., additional, Prikhozhdenko, Ekaterina S., additional, Verkhovskii, Roman A., additional, Mikaelyan, Arsen S., additional, Kotelevtsev, Yuri V., additional, and Sukhorukov, Gleb B., additional

Published
2022
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24. Corrections to “Detection of Melanoma Cells in Whole Blood Samples Using Spectral Imaging and Optical Clearing”

Author

Dyachenko Timoshina, Polina, primary, Dolotov, Leonid, additional, Lazareva, Ekaterina, additional, Kozlova, Anastasia, additional, Inozemtseva, Olga, additional, Verkhovskii, Roman, additional, Afanaseva, Galina, additional, Shushunova, Natalia, additional, Tuchin, Valery, additional, Galanzha, Ekaterina, additional, and Zharov, Vladimir, additional

Published
2021
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25. Detection of Melanoma Cells in Whole Blood Samples Using Spectral Imaging and Optical Clearing

Author

Dyachenko, Polina A., primary, Dolotov, Leonid E., additional, Lazareva, Ekaterina N., additional, Kozlova, Anastasia A., additional, Inozemtseva, Olga A., additional, Verkhovskii, Roman A., additional, Afanaseva, Galina A., additional, Shushunova, Natalia A., additional, Tuchin, Valery V., additional, Galanzha, Ekaterina I., additional, and Zharov, Vladimir P., additional

Published
2021
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26. pH-sensitive dairy-derived hydrogels with a prolonged drug release profile for cancer treatment

Author

Mayorova, Oksana, Jolly, Ben, Verkhovskii, Roman, Plastun, Valentina, Sindeeva, Olga, Douglas, Timothy, Mayorova, Oksana, Jolly, Ben, Verkhovskii, Roman, Plastun, Valentina, Sindeeva, Olga, and Douglas, Timothy

Abstract

A novel versatile biocompatible hydrogel of whey protein isolate (WPI) and two types of tannic acid (TAs) was prepared by crosslinking of WPI with TAs in a one-step method at high temperature for 30 min. WPI is one common protein-based preparation which is used for hydrogel formation. The obtained WPI-TA hydrogels were in disc form and retained their integrity after sterilization by autoclaving. Two TA preparations of differing molecular weight and chemical structure were compared, namely a polygalloyl glucose-rich extract-ALSOK 02-and a polygalloyl quinic acid-rich extract-ALSOK 04. Hydrogel formation was observed for WPI solutions containing both preparations. The swelling characteristics of hydrogels were investigated at room temperature at different pH values, namely 5, 7, and 9. The swelling ability of hydrogels was independent of the chemical structure of the added TAs. A trend of decrease of mass increase (MI) in hydrogels was observed with an increase in the TA/WPI ratio compared to the control WPI hydrogel without TA. This dependence (a MI decrease-TA/WPI ratio) was observed for hydrogels with different types of TA both in neutral and acidic conditions (pH 5.7). Under alkaline conditions (pH 9), negative values of swelling were observed for all hydrogels with a high content of TAs and were accompanied by a significant release of TAs from the hydrogel network. Our studies have shown that the release of TA from hydrogels containing ALSOK04 is higher than from hydrogels containing ALSOK 02. Moreover, the addition of TAs, which display a strong anti-cancer effect, increases the cytotoxicity of WPI-TAs hydrogels against the Hep-2 human laryngeal squamous carcinoma (Hep-2 cells) cell line. Thus, WPI-TA hydrogels with prolonged drug release properties and cytotoxicity effect can be used as anti-cancer scaffolds.

Published
2021

32. Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow

Author

Verkhovskii, Roman A., primary, Kozlova, Anastasiia A., additional, Sindeeva, Olga A., additional, Kozhevnikov, Ilya O., additional, Prikhozhdenko, Ekaterina S., additional, Mayorova, Oksana A., additional, Grishin, Oleg V., additional, Makarkin, Mikhail A., additional, Ermakov, Alexey V., additional, Abdurashitov, Arkady S., additional, Tuchin, Valery V., additional, and Bratashov, Daniil N., additional

Published
2020
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33. Macrophage In Vitroand In VivoTracking via Anchored Microcapsules

Author

Sapach, Anastasiia Yu., Sindeeva, Olga A., Nesterchuk, Mikhail V., Tsitrina, Alexandra A., Mayorova, Oksana A., Prikhozhdenko, Ekaterina S., Verkhovskii, Roman A., Mikaelyan, Arsen S., Kotelevtsev, Yuri V., and Sukhorukov, Gleb B.

Abstract

A new promising trend in personalized medicine is the use of autologous cells (macrophages or stem cells) for cell-based therapy and also as a “Trojan horse” for targeted delivery of a drug carrier. The natural ability of macrophages for chemotaxis allows them to deliver cargo to the damaged area, significantly reducing side effects on healthy organ tissues. Therefore, it is important to develop tools to track their behavior in the organism. While labeled containers can serve as anchored tags for imaging macrophages in vivo, they can affect the properties and functions of macrophages. This work demonstrates that 3 μm sized capsules based on biocompatible polyelectrolytes and fluorescently labeled with both Cy7 and RITC dyes do not affect cell functionalization in vitro, such as viability, proliferation, and movement of transformed monocyte/macrophage-like cells (RAW 264.7) and primary bone marrow derived macrophages (BMDM) at maximal loading of five capsules per cell. In addition, capsules allowed fluorescent detection of ex vivoloaded cells 24 h after the tail vein injection in vivoand visualization of microcapsule-laden macrophages ex vivousing confocal microscopy. We have delivered about 62.5% of injected BMDM containing 12.5 million capsules with 3.75 μg of high-molecular-weight cargo (0.3 pg/capsule) to the liver. Our results demonstrate that 3 μm polyelectrolyte fluorescently labeled microcapsules can be used for safe macrophage loading, allowing cell tracking and drug delivery, which will facilitate development of macrophage-based cell therapy protocols.

Published
2022
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35. In Vitro Bioeffects of Polyelectrolyte Multilayer Microcapsules Post-Loaded with Water-Soluble Cationic Photosensitizer

Author

Ermakov, Alexey V., primary, Verkhovskii, Roman A., additional, Babushkina, Irina V., additional, Trushina, Daria B., additional, Inozemtseva, Olga A., additional, Lukyanets, Evgeny A., additional, Ulyanov, Vladimir J., additional, Gorin, Dmitry A., additional, Belyakov, Sergei, additional, and Antipina, Maria N., additional

Published
2020
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38. Effect of Systemic Polyelectrolyte Microcapsule Administration on the Blood Flow Dynamics of Vital Organs

Author

Sindeeva, Olga A., primary, Verkhovskii, Roman A., additional, Abdurashitov, Arkady S., additional, Voronin, Denis V., additional, Gusliakova, Olga I., additional, Kozlova, Anastasiia A., additional, Mayorova, Oksana A., additional, Ermakov, Aleksey V., additional, Lengert, Ekaterina V., additional, Navolokin, Nikita A., additional, Tuchin, Valery V., additional, Gorin, Dmitry A., additional, Sukhorukov, Gleb B., additional, and Bratashov, Daniil N., additional

Published
2019
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45. New Frontiers in Diagnosis and Therapy of Circulating Tumor Markers in Cerebrospinal Fluid In Vitro and In Vivo.

Author

Sindeeva, Olga A., Verkhovskii, Roman A., Sarimollaoglu, Mustafa, Afanaseva, Galina A., Fedonnikov, Alexander S., Osintsev, Evgeny Yu., Kurochkina, Elena N., Gorin, Dmitry A., Deyev, Sergey M., Zharov, Vladimir P., and Galanzha, Ekaterina I.

Subjects
*CEREBROSPINAL fluid, *CEREBROSPINAL fluid examination, *TUMOR markers, *CIRCULATING tumor DNA, *CENTRAL nervous system, *TARGETED drug delivery
Abstract

One of the greatest challenges in neuro-oncology is diagnosis and therapy (theranostics) of leptomeningeal metastasis (LM), brain metastasis (BM) and brain tumors (BT), which are associated with poor prognosis in patients. Retrospective analyses suggest that cerebrospinal fluid (CSF) is one of the promising diagnostic targets because CSF passes through central nervous system, harvests tumor-related markers from brain tissue and, then, delivers them into peripheral parts of the human body where CSF can be sampled using minimally invasive and routine clinical procedure. However, limited sensitivity of the established clinical diagnostic cytology in vitro and MRI in vivo together with minimal therapeutic options do not provide patient care at early, potentially treatable, stages of LM, BM and BT. Novel technologies are in demand. This review outlines the advantages, limitations and clinical utility of emerging liquid biopsy in vitro and photoacoustic flow cytometry (PAFC) in vivo for assessment of CSF markers including circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), microRNA (miRNA), proteins, exosomes and emboli. The integration of in vitro and in vivo methods, PAFC-guided theranostics of single CTCs and targeted drug delivery are discussed as future perspectives. [ABSTRACT FROM AUTHOR]

Published
2019
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46. CaCO 3 -based carriers with prolonged release properties for antifungal drug delivery to hair follicles.

Author

Saveleva MS, Lengert EV, Verkhovskii RA, Abalymov AA, Pavlov AM, Ermakov AV, Prikhozhdenko ES, Shtykov SN, and Svenskaya YI

Subjects
Animals, Calcium Carbonate, Drug Carriers metabolism, Drug Delivery Systems, Excipients, Rats, Skin Absorption, Water, Antifungal Agents pharmacology, Hair Follicle
Abstract

Superficial fungal infections are of serious concern worldwide due to their morbidity and increasing distribution across the globe in this era of growing antimicrobial resistance. The delivery of antifungals to the target regions of the skin and sustaining the effective drug concentration are essential for successful treatment of such mycoses. Topical formulations get extra benefits here if they penetrate into the hair follicles since fungal hyphae can proliferate and produce spores in such reservoirs. We designed a novel particulate system for the encapsulation and intrafollicular delivery of griseofulvin (Gf) antifungal drug, which is water-insoluble and currently commercially available in oral dosage forms. Micron-sized calcium carbonate (vaterite) carriers containing 25 ± 3% (w/w) of Gf were prepared via the wet chemical method. The successful in vivo transportation of the carriers into the hair follicles of rats was demonstrated using scanning electron and confocal laser scanning microscopy. In addition, we introduced an approach toward Gf release prolongation for the proposed system. The stabilizing coatings were formed on the surface of the obtained particles via the layer-by-layer technique. The formulations displayed sufficient biocompatibility and good cellular uptake in contact with fibroblast cells in vitro . Four different coatings were tested for their preserving ability in the course of continued carrier incubation in the model media. The best release prolonging formulation liberated 38% of the loaded Gf during 5 days, while the uncoated carriers demonstrated more than 50% drug release within the first 24 h in water. To assess the in vivo release properties, free Gf drug and Gf-loaded carriers (uncovered and covered with the stabilizing shell) were administered topically in rats and the drug excretion profiles were further studied. By comparing the daily Gf levels in urine, we verified the sustained effect (longer than a week) of the stabilizing shell formed on the carrier surface. Conversely, the application of the free drug did not provide reliable Gf detection for this period. These findings open new prospects for the efficiency enhancement of topical therapeutics. Importantly, the elaborated system could be adapted for the dermal delivery of various water-insoluble drugs beyond the scope of antifungal therapy.

Published
2022
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