1. Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus
- Author
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Amanda Bezerra Bertolini, Patrícia Jacqueline Thyssen, Paulo Eduardo Brandão, Aline Marrara Prado, Sheila Oliveira de Souza Silva, Mateus de Souza Ribeiro Mioni, Fábio Lucas Rezende de Gouvea, José Carlos de Figueiredo Pantoja, Helio Langoni, Felipe Freitas Guimarães, Sâmea Fernandes Joaquim, Simony Trevizan Guerra, Domingos da Silva Leite, Vera Mores Rall, Rodrigo Tavanelli Hernandes, Simone Baldini Lucheis, Gabriel Augusto Marques Rossi, and Márcio Garcia Ribeiro
- Subjects
General Medicine ,Applied Microbiology and Biotechnology ,Biotechnology - Abstract
AimsWe aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.Methods and ResultsWe collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens.ConclusionsOur findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.
- Published
- 2023
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