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Breast cancer therapy using anticancer bioactive compounds derived from natural products as adjuvant treatment has gained recognition due to expensive and toxic conventional chemotherapeutic drugs. The whole plant of Anastatica hierochuntica (L.) (A. hierochuntica) has been investigated for its pharmacologically important anticancer properties but without categorizing the biological activities of the plant parts. We assessed the anticancer potential of different parts of A. hierochuntica (seeds, stems and leaves) and explored their mechanisms of action using the human breast cancer cell line, MCF-7. Currently, we investigated the antiproliferative effects of methanolic (MSD, MST, ML) and aqueous (ASD, AST, AL) extracts of A. hierochuntica plant parts on the MCF-7 cells using cell viability assays. Flow cytometry, Western Blot, DNA fragmentation, and gene expression assays were performed to evaluate apoptosis and cell cycle regulatory proteins. The results indicate that the methanolic and aqueous extracts decreased MCF-7 cell viability in a dose-dependent manner. The induction of apoptosis was observed in all the methanolic and aqueous-treated MCF-7 cells. The cell death process was confirmed by the visualization of DNA fragmentation and cleavage of the intrinsic apoptotic pathways, caspase-9 and caspase-3, the key enzyme causing apoptosis hallmarks. In addition, the most pro-apoptotic extracts, ASD and ML, up-regulated the expression of pro-apoptotic Bax, tumor suppressor TP53 genes and the cyclin inhibitor CDKN1A gene. In conclusion, of the aqueous and methanolic extracts of A. hierochuntica plant parts exerting antiproliferative effects through the induction of apoptosis in breast cancer MCF-7 cells, ASD and ML extracts were the most promising natural-based drugs for the treatment of breast cancer.

Rhizophora mucronata is a species of mangrove widely distributed in Indian mangrove forest. It seems to be more tolerant of inundation than other mangrove species and often forms an evergreen fringe to mangrove areas. In present study, the methanolic extract of different parts of Rhizophora mucronata, their antioxidant properties along with primary, secondary metabolites and proximate composition, were tested. Better antioxidant activity was gained by methanolic extract of leaf than root and bark extract. Antioxidant potential of the extracts was analyzed as contents of total phenols and flavonoids; radical scavenging activity by the DPPH methods, NO and H2O2 and primary and secondary metabolites assay and the results of antioxidative activities from the R. mucronata plant. The total content of phenols and flavonoids in the methanol extracts of the studied species positively correlated with their antioxidant properties, confirmed their major role in antioxidant activity of these R. mucronata.

With the intention of assessing the neuro-imaging, clarifying the percentages of symptomatic, idiopathic and cryptogenic cases and identifying the underlying causes, records were reviewed retrospectively of 30 children with infantile spasm admitted to Hamad Medical Corporation, Doha, Qatar. Twenty-three patients were symptomatic, four idiopathic and three cryptogenic. Brain CT and MRI scans of 13 cases detected similar neuro-imaging findings in nine cases while MRI provided more information in another four cases. Three patients had normal neuro-images despite persistently abnormal EEGs. We conclude that MRI scans are superior to CT scans in detecting areas of cortical dysgenesis, disorders of neuronal migration, or disorders of myelination although more advanced studies are required for patients with persistently abnormal EEGs but normal MRIs.