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291 results on '"Venkataravanappa, V."'

41. Molecular characterization of ginger genotypes using RAPD and SSR markers

Author

Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, and Venkataravanappa V

Subjects
ginger, molecular markers, monomorphic, polymorphic, Plant culture, SB1-1110
Abstract

Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into different groups which showed that, each individual genotype is having wider variability or it might be due to the genetic similarity existing among them.

Published
2022
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46. Molecular characterization of begomovirus and DNA satellites associated with mosaic and leaf curl disease of Jamaica cherry (Muntingia calabura)in India: Uncovering a new host for chilli leaf curl India virus

Author

Prasad, K S Uday Durga, Kavya, S S, Sindhura, Kopparthi Amrutha Valli, Muttappagol, Mantesh, Kruthika, R, Basha, C. R. Jahir, Shankarappa, K. S., Venkataravanappa, V., and Lakshminarayana Reddy, C. N.

Abstract

Begomoviruses, member of the Geminiviridaefamily, are responsible for significant economic losses in crops worldwide. Chilli leaf curl India virus (ChiLCINV) is a well-known begomovirus that causes leaf curl disease, primarily affecting plants in the Solanaceaefamily. In this study, sample from a Jamaica cherry (Muntingia calabura) tree showing typical begomovirus symptoms of mosaic and leaf curling was collected from Nagavara village in the Bengaluru Rural district of Karnataka State, India. The collected sample was designated as the MUT-1 isolate. The association of the begomovirus (DNA-A) and betasatellites with the sample was confirmed by PCR using begomovirus-specific primers, resulting in the expected amplicons of approximately 1.2 kb and 1.3 kb, respectively. No amplification was obtained for DNA-B and alphasatellite specific primers. The complete genome sequence of DNA-A of begomovirus isolate MUT-1 was obtained through rolling circle amplification and compared with other begomoviruses using Sequence Demarcation Tool which revealed that, DNA-A of MUT-1 isolate, (Acc.No. PP475538) showed maximum nucleotide (nt) identity of 98.7–99.4% with chilli leaf curl India virus. Further, sequence of betasattelite (Acc.No. PP493212) of this isolate shared maximum nt identity of 86.5–100% with tomato leaf curl Bangladesh betasatellite (ToLCBDB). Recombination and GC plot analysis showed that the presence of two and three intraspecific recombination event in DNA-A and betasatellite genomic regions, respectively and are derived from the previously reported begomoviruses. This study presents one more evidence of expanding host range for begomoviruses and first record of begomovirus associated with mosaic and leaf curl disease of Jamaica cherry (M. calabura) from India.

Published
2024
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