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17 results on '"Vencia, Walter"'

3. Evidence of Antimicrobial Resistance and Presence of Pathogenicity Genes in Yersinia enterocolitica Isolate from Wild Boars

Author

Modesto, Paola, primary, De Ciucis, Chiara Grazia, additional, Vencia, Walter, additional, Pugliano, Maria Concetta, additional, Mignone, Walter, additional, Berio, Enrica, additional, Masotti, Chiara, additional, Ercolini, Carlo, additional, Serracca, Laura, additional, Andreoli, Tiziana, additional, Dellepiane, Monica, additional, Adriano, Daniela, additional, Zoppi, Simona, additional, Meloni, Daniela, additional, and Razzuoli, Elisabetta, additional

Published
2021
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10. Effects of thermal treatment on walnut detection and allergenicity.

Author

Vencia, Walter, Minale, Paola, Migone, Laura, Lazzara, Fabrizio, Vito, Guendalina, Ferrari, Angelo, and Razzuoli, Elisabetta

Subjects
*WALNUT, *HEAT treatment, *FOOD safety, *ENZYME-linked immunosorbent assay, *FOOD allergy
Abstract

BACKGROUND Peanuts and tree nut allergies pose an increasing food safety problem. The aim of our study was to test the accuracy of different commercial enzyme‐linked immunosorbent assay (ELISA) kits in the detection of the presence of walnuts in untreated and heat exposed food samples. The effects of thermal treatment of samples were evaluated by exposing walnuts to different heat treatments. All samples were first analysed by two different commercial ELISA assays. Then, we performed a skin prick test (SPT) on nine patients with proven nut allergy using small walnut pieces from raw and treated samples. RESULTS: The presence of nuts proteins in thermally processed foods was not accurately detected by ELISA kits. All patients had a positive SPT reaction with raw walnut, while thermal treatments affected walnut allergenicity. The ELISA test gives a negative result in the case of strong thermal treatment, but at the same time allergic subjects react positively to stimulation with the same sample. CONCLUSION: This study suggests that commercial ELISA kits may not be able to accurately determine the amount of proteins present in thermally processed foods due to changes in the solubility and immunoreactivity of the target proteins. Finally, the clinical results highlight that thermal treatment might induce a reduction in walnut allergenicity. © 2018 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Published
2019
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13. Food allergens: state of the art in Piedmont region in the period 2011-2012

Author

Barbaro, Antonio, primary, Rubinetti, Francesca, additional, Galleggiante Crisafulli, Annamaria, additional, Radaelli, Maria Cristina, additional, Chiavacci, Laura, additional, Bianchi, Daniela Manila, additional, Adriano, Daniela, additional, Zuccon, Fabio, additional, Fragassi, Sandra, additional, Buonincontro, Giuseppina, additional, Vencia, Walter, additional, and Decastelli, Lucia, additional

Published
2014
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16. In Vitro Susceptibility of Multidrug Resistant Strains of Salmonellato Essential Oils

Author

Listorti, Valeria, Battistini, Roberta, Ercolini, Carlo, Tramuta, Clara, Razzuoli, Elisabetta, Vencia, Walter, Decastelli, Lucia, Gallina, Silvia, Masotti, Chiara, and Serracca, Laura

Abstract

Antimicrobial resistance has become a global threat to public health. There is a critical need to find new antimicrobial substances from natural sources. The aim of this study was to investigate the antimicrobial activity of essential oils (EOs) obtained from Origanum vulgare, Thymus serpyllum, Thymus vulgaris, and Melaleuca alternifoliaagainst multidrug resistant strains of Salmonellaisolated from samples of diverse animal origin. The strains were biochemically identified, serotyped, and characterized for their antimicrobial resistance profiles. The antimicrobial activity of the EOs against the strains was evaluated using the Kirby-Bauer diffusion method, followed by determination of the minimal inhibitory concentration and minimum bactericidal concentrations. The EOs of T. serpyllumand O. vulgare, which contain carvacrol as the main compound, show excellent antimicrobial activity.

Published
2020
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17. Development and validation of species-specific molecular diagnostic tool for Opisthorchis felineus (Digenea, Opisthorchiidae) metacercariae

Author

Monica Caffara, Marino Prearo, Andrea Gustinelli, Laura Serracca, Marialetizia Fioravanti, Walter Vencia, Irene Rossini, Caffara, Monica, Serracca, Laura, Gustinelli, Andrea, Vencia, Walter, Rossini, Irene, Prearo, Marino, and Fioravanti, Maria L.

Subjects
0301 basic medicine, 030231 tropical medicine, Cyprinidae, Zoology, Microbiology, Opisthorchiasis, Polymerase Chain Reaction, Digenea, Opisthorchis felineu, 03 medical and health sciences, Fish Diseases, 0302 clinical medicine, Species Specificity, Validation, medicine, Helminths, Parasite hosting, Animals, Metacercariae, Opisthorchis felineus, Pathology, Molecular, Safety, Risk, Reliability and Quality, DNA Primers, Flatworm, biology, Ecology, Opisthorchis, Fish fillet, General Medicine, 030108 mycology & parasitology, biology.organism_classification, medicine.disease, Opisthorchiidae, Species-specific primer, Food Science
Abstract

Opisthorchis felineus (family Opisthorchiidae) is a parasitic flatworm representing a serious threat to humans in some countries. Opisthorchiasis occurs after consumption of raw or undercooked cyprinid fish infected by the metacercarial stage of the parasite. Due to its small size, detection of the parasite in fish fillet is time-consuming and difficult. Furthermore, isolated metacercariae can be identified to genus but not to species level using morphological features and molecular techniques are necessary. In this work, we describe the development of primers for a diagnostic PCR amplification of a 254-bp fragment of the cytochrome c oxidase I in the mitochondrion of Opisthorchis felineus metacercariae isolated from fish fillet, together with a validation protocol for this method.

Published
2016

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