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1. Fungal β-Glucosidases

Author

Mathew, Reshma M., primary, Sankar, Meena, additional, Sreeja-Raju, Athira, additional, Prasannakumari, Adarsh Velayudhanpillai, additional, Anoop Puthiyamadam, Athulya, additional, Christopher, Meera, additional, Jose, Jedy, additional, Rebinro, Valan, additional, and Sukumaran, Rajeev K., additional

Published
2024
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6. Sono-Assisted Alkali and Dilute Acid Pretreatment of Phragmites karka (Tall Reed Grass) to Enhance Enzymatic Digestibility for Bioethanol Conversion

Author

Sabeela Beevi Ummalyma, Dinabandhu Sahoo, Anoop Pudiyamadam, Velayudhanpillai Prasannakumari Adarsh, Rajeev K. Sukumaran, Thallada Bhaskar, and Ajay Parida

Subjects
Phragmites, sonication, pretreatment, Chilika Lake, Loktak lake, General Works
Abstract

Phragmites is the tallest energy crop found as an invasive species worldwide and considered as waste biomass. The present study evaluated the potential of the aquatic biomass Phragmites karka grown from two different lakes as feedstock for biofuel production. A comparative study of biomass was conducted from Chilika Lake and Loktak Lake, India. The methodology involves dilute acid, sono-assisted alkali pretreatment, and hydrolyzed biomass with commercial cellulase. SEM, XRD, and FTIR analysis were performed for the biomass physicochemical studies and confirmed that alterations occurred in the biomass structure, assisting the hydrolysis process. The enzymatic hydrolysis result showed that the highest of reducing sugar yield of 79% was obtained from biomass loading of 10% and 1% w/v alkali with a sonication frequency of 20 kHz for 25 min. Acid pretreatment released maximal reducing sugar yield of 73% attained from biomass loading of 20% and 0.5% w/v acid. Composition analysis of biomass showed that cellulose content increased from 36% to 46%. Sono-assisted alkali pretreatment solubilized 40% of lignin content compared to untreated biomass. Final ethanol recovery from the biomass is 78% fermentation efficiency from glucose. The data indicate that exploiting tall reed grass as a bioenergy raw material can be a viable approach for sustainable utilization of invasive grass/waste biomass for biorefineries, which helps control invasive weeds and management of waste.

Published
2021
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7. Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2

Author

Salwa Naushin, Viren Sardana, Rajat Ujjainiya, Nitin Bhatheja, Rintu Kutum, Akash Kumar Bhaskar, Shalini Pradhan, Satyartha Prakash, Raju Khan, Birendra Singh Rawat, Karthik Bharadwaj Tallapaka, Mahesh Anumalla, Giriraj Ratan Chandak, Amit Lahiri, Susanta Kar, Shrikant Ramesh Mulay, Madhav Nilakanth Mugale, Mrigank Srivastava, Shaziya Khan, Anjali Srivastava, Bhawana Tomar, Murugan Veerapandian, Ganesh Venkatachalam, Selvamani Raja Vijayakumar, Ajay Agarwal, Dinesh Gupta, Prakash M Halami, Muthukumar Serva Peddha, Gopinath M Sundaram, Ravindra P Veeranna, Anirban Pal, Vinay Kumar Agarwal, Anil Ku Maurya, Ranvijay Kumar Singh, Ashok Kumar Raman, Suresh Kumar Anandasadagopan, Parimala Karuppanan, Subramanian Venkatesan, Harish Kumar Sardana, Anamika Kothari, Rishabh Jain, Anupama Thakur, Devendra Singh Parihar, Anas Saifi, Jasleen Kaur, Virendra Kumar, Avinash Mishra, Iranna Gogeri, Geethavani Rayasam, Praveen Singh, Rahul Chakraborty, Gaura Chaturvedi, Pinreddy Karunakar, Rohit Yadav, Sunanda Singhmar, Dayanidhi Singh, Sharmistha Sarkar, Purbasha Bhattacharya, Sundaram Acharya, Vandana Singh, Shweta Verma, Drishti Soni, Surabhi Seth, Sakshi Vashisht, Sarita Thakran, Firdaus Fatima, Akash Pratap Singh, Akanksha Sharma, Babita Sharma, Manikandan Subramanian, Yogendra S Padwad, Vipin Hallan, Vikram Patial, Damanpreet Singh, Narendra Vijay Tripude, Partha Chakrabarti, Sujay Krishna Maity, Dipyaman Ganguly, Jit Sarkar, Sistla Ramakrishna, Balthu Narender Kumar, Kiran A Kumar, Sumit G Gandhi, Piyush Singh Jamwal, Rekha Chouhan, Vijay Lakshmi Jamwal, Nitika Kapoor, Debashish Ghosh, Ghanshyam Thakkar, Umakanta Subudhi, Pradip Sen, Saumya Ray Chaudhury, Rashmi Kumar, Pawan Gupta, Amit Tuli, Deepak Sharma, Rajesh P Ringe, Amarnarayan D, Mahesh Kulkarni, Dhansekaran Shanmugam, Mahesh S Dharne, Sayed G Dastager, Rakesh Joshi, Amita P Patil, Sachin N Mahajan, Abujunaid Habib Khan, Vasudev Wagh, Rakesh Kumar Yadav, Ajinkya Khilari, Mayuri Bhadange, Arvindkumar H Chaurasiya, Shabda E Kulsange, Krishna Khairnar, Shilpa Paranjape, Jatin Kalita, Narahari G Sastry, Tridip Phukan, Prasenjit Manna, Wahengbam Romi, Pankaj Bharali, Dibyajyoti Ozah, Ravi Kumar Sahu, Elapavalooru VSSK Babu, Rajeev Sukumaran, Aiswarya R Nair, Prajeesh Kooloth Valappil, Anoop Puthiyamadam, Adarsh Velayudhanpillai, Kalpana Chodankar, Samir Damare, Yennapu Madhavi, Ved Varun Aggarwal, Sumit Dahiya, Anurag Agrawal, Debasis Dash, and Shantanu Sengupta

Subjects
SARS-CoV2, antibody stability, sero-prevalence, neutralizing antibody, Medicine, Science, Biology (General), QH301-705.5
Abstract

To understand the spread of SARS-CoV2, in August and September 2020, the Council of Scientific and Industrial Research (India) conducted a serosurvey across its constituent laboratories and centers across India. Of 10,427 volunteers, 1058 (10.14%) tested positive for SARS-CoV2 anti-nucleocapsid (anti-NC) antibodies, 95% of which had surrogate neutralization activity. Three-fourth of these recalled no symptoms. Repeat serology tests at 3 (n = 607) and 6 (n = 175) months showed stable anti-NC antibodies but declining neutralization activity. Local seropositivity was higher in densely populated cities and was inversely correlated with a 30-day change in regional test positivity rates (TPRs). Regional seropositivity above 10% was associated with declining TPR. Personal factors associated with higher odds of seropositivity were high-exposure work (odds ratio, 95% confidence interval, p value: 2.23, 1.92–2.59,

Published
2021
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8. Development of a nitrogen-doped 2D material for tribological applications in the boundary-lubrication regime

Author

Shende Rashmi Chandrabhan, Velayudhanpillai Jayan, Somendra Singh Parihar, and Sundara Ramaprabhu

Subjects
friction, lubrication, nanolubricant, nitrogen-doped reduced graphene oxide, tribology, wear, Technology, Chemical technology, TP1-1185, Science, Physics, QC1-999
Abstract

The present paper describes a facile synthesis method for nitrogen-doped reduced graphene oxide (N-rGO) and the application of N-rGO as an effective additive for improving the tribological properties of base oil. N-rGO has been characterized by different characterization techniques such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and Raman spectroscopy. N-rGO-based nanolubricants are prepared and their tribological properties are studied using a four-ball tester. The nanolubricants show excellent stability over a period of six months and a significant decrease in coefficient of friction (25%) for small amounts of N-rGO (3 mg/L). The improvement in tribological properties can be attributed to the sliding mechanism of N-rGO accompanied by the high mechanical strength of graphene. Further, the nanolubricant is prepared at large scale (700 liter) and field trials are carried out at one NTPC thermal plant in India. The implementation of the nanolubricant in an induced draft (ID) fan results in the remarkable decrease in the power consumption.

Published
2017
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9. Bioprocess development of 2, 3-butanediol production using agro-industrial residues

Author

Sulfath Hakkim Hazeena, Narasinha J. Shurpali, Henri Siljanen, Reijo Lappalainen, Puthiyamdam Anoop, Velayudhanpillai Prasannakumari Adarsh, Raveendran Sindhu, Ashok Pandey, and Parameswaran Binod

Subjects
Fermentation, Bioengineering, Biomass, General Medicine, Carbon Dioxide, Butylene Glycols, Sugars, Biotechnology
Abstract

The valorization of agricultural and industrial wastes for fuel and chemical production benefits environmental sustainability. 2, 3-Butanediol (2,3-BDO) is a value-added platform chemical covering many industrial applications. Since the global market is increasing drastically, production rates have to increase. In order to replace the current petroleum-based 2,3-BDO production, renewable feedstock's ability has been studied for the past few decades. This study aims to find an improved bioprocess for producing 2,3-BDO from agricultural and industrial residues, consequently resulting in a low CO2 emission bioprocess. For this, screening of 13 different biomass samples for hydrolyzable sugars has been done. Alkali pretreatment has been performed with the processed biomass and enzyme hydrolysis performed using commercial cellulase. Among all biomass hydrolysate oat hull and spruce bark biomass could produce the maximum amount of total reducing sugars. Later oat hull and spruce bark biomass with maximum hydrolyzable sugars have been selected for submerged fermentation studies using Enterobacter cloacae SG1. After fermentation, 37.59 and 26.74 g/L of 2,3-BDO was obtained with oat hull and spruce bark biomass, respectively. The compositional analysis of each step of biomass processing has been performed and changes in each component have been evaluated. The compositional analysis has revealed that biomass composition has changed significantly after pretreatment and hydrolysis leading to a remarkable release of sugars which can be utilized by bacteria for 2,3-BDO production. The results have been found to be promising, showing the potential of waste biomass residues as a low-cost raw material for 2,3-BDO production and thus a new lead in an efficient waste management approach for less CO2 emission. Graphical Abstract

Published
2022
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11. Ethanol production by a filamentous fungal strain Byssochlamys fulva AM130 under alternating aerobic and oxygen-limited conditions

Author

Rajeev K. Sukumaran, Abraham Mathew, Ashok Pandey, Anoop Puthiyamadam, Prajeesh Kooloth-Valappil, Jayaram Krishnamoorthy, and Velayudhanpillai Prasannakumari Adarsh

Subjects
Ethanol, biology, Fungus, Cellulase, Xylose, biology.organism_classification, chemistry.chemical_compound, chemistry, Xylanase, biology.protein, Fermentation, Ethanol fuel, Food science, Mycelium
Abstract

Byssochlamys fulva AM130, a novel strain of filamentous fungus, could produce ethanol from glucose, xylose, and alkali pretreated rice straw (PRS), while the efficiencies were very low with PRS. Ethanol production of 11.84 g/L was attained by the fungus when grown in glucose, indicating that the limitations while growing on PRS were related to low hydrolytic efficiency. Enzyme profiling of the fungus showed 365 IU/ml of beta-glucosidase and 89 IU/ml of xylanase activity, while endoglucanase and filter paper activity were negligible, which accounts for the low hydrolytic efficiency. The fungus could survive for extended periods under oxygen-limited conditions and produce ethanol. The fungal mycelia could also be used for repeated cycles of anaerobic fermentation, wherein the ethanol yield improved with each consecutive cycle.

Published
2020
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12. Value-addition of water hyacinth and para grass through pyrolysis and hydrothermal liquefaction

Author

Anoop Puthiyamadam, Thallada Bhaskar, Yenumala Sudhakara Reddy, Vaibhav Dhyani, Ayushi Awasthi, Jitendra Kumar, Kiran Kumar Mallapureddy, Rajeev K. Sukumaran, Dinabandhu Sahoo, Sabeela Beevi Ummalyma, Bijoy Biswas, and Velayudhanpillai Prasannakumari Adarsh

Subjects
Morphology (linguistics), biology, Chemistry, Hyacinth, 020209 energy, Process Chemistry and Technology, Materials Science (miscellaneous), Biomass, chemistry.chemical_element, 02 engineering and technology, lcsh:Chemical technology, biology.organism_classification, Nitrogen, Catalysis, Hydrothermal liquefaction, Fuel Technology, 020401 chemical engineering, Yield (chemistry), 0202 electrical engineering, electronic engineering, information engineering, lcsh:TP1-1185, 0204 chemical engineering, Pyrolysis, Macromolecule, Nuclear chemistry
Abstract

Hydrothermal liquefaction (HTL) and pyrolysis (Py) of Loktak lake biomass mixture of water hyacinth (WH) and para grass (PG) were carried out at 260–300 °C and 300–500 °C to compare the products yield and chemical characteristics of the products. In case of HTL, the maximum bio-oil yield was obtained 13.34 wt.% at 280 °C while for Py, the maximum bio-oil yield was observed 38.8 wt.% at 350 °C. The obtained bio-oils and bio-chars were analyzed using GC–MS, FT-IR, NMR, TGA, TOC, and SEM. GC–MS analysis of the bio-oils were showed that the HTL bio-oil majorly contains of nitrogen containing compounds whereas the Py bio-oil contains majority of phenolic compounds. Other compounds like ketones, alcohols, acids were also observed in bio-oil. Higher intensity broad band at 3300–3500 cm−1 was observed in the Py bio-oil compared to HTL bio-oil. TGA and proximate analysis of bio-char revealed the higher devolatilization occured during the HTL compared to Py process. The surface morphology of the HTL bio-char was found to be rough and fragmented as compared to the pyrolysis bio-char, clearly showing the biomass macromolecules breakdown differently in HTL and Py process. Keywords: Hydrothermal liquefaction, Pyrolysis, Aquatic biomass, Bio-oil, Functional compounds, Bio-char

Published
2019
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13. Author response: Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2

Author

Mrigank Srivastava, Subramanian Venkatesan, Vipin Hallan, Shabda E Kulsange, Raju Khan, Rekha Chouhan, Narendra Vijay Tripude, Sakshi Vashisht, Rahul Chakraborty, Jatin Kalita, Selvamani Raja Vijayakumar, Jasleen Kaur, Susanta Kar, R.V.K. Singh, Abujunaid Habib Khan, Sundaram Acharya, Shrikant R. Mulay, Dayanidhi Singh, Nitika Kapoor, Pinreddy Karunakar, Sachin N Mahajan, Rohit Yadav, Anjali Srivastava, Prakash M. Halami, Anirban Pal, Anamika Kothari, Bhawana Tomar, Babita Sharma, Anil Ku Maurya, Mahesh J. Kulkarni, Akash Pratap Singh, Arvindkumar H Chaurasiya, Shantanu Sengupta, Saumya Ray Chaudhury, Partha Chakrabarti, Viren Sardana, Anas Saifi, Prasenjit Manna, Aiswarya R Nair, Mahesh S. Dharne, Ravindra P Veeranna, Tridip Phukan, Muthukumar Serva Peddha, Amita P Patil, Birendra Singh Rawat, Gopinath M Sundaram, Mayuri Bhadange, Shweta Verma, Umakanta Subudhi, Vijay Lakshmi Jamwal, Vasudev Wagh, Firdaus Fatima, Ganesh Venkatachalam, Debashish Ghosh, Avinash Mishra, Manikandan Subramanian, Rintu Kutum, Rajesh P. Ringe, Sumit G. Gandhi, Purbasha Bhattacharya, Devendra Singh Parihar, Shilpa Paranjape, Dipyaman Ganguly, Yennapu Madhavi, Shalini Pradhan, Yogendra Padwad, Parimala Karuppanan, Sarita Thakran, Ved Varun Aggarwal, Pawan Gupta, Piyush Singh Jamwal, Pankaj Bharali, Satyartha Prakash, Amit Tuli, Jit Sarkar, Samir Damare, Ajay Agarwal, Rakeshkumar Yadav, Balthu Narender Kumar, Anoop Puthiyamadam, Giriraj R. Chandak, Murugan Veerapandian, Debasis Dash, Ajinkya Khilari, Sumit Dahiya, Mahesh Anumalla, Sharmistha Sarkar, Kalpana Chodankar, Anupama Thakur, Ghanshyam Thakkar, Narahari G Sastry, Iranna Gogeri, Deepak Sharma, Surabhi Seth, Pradip Sen, Harish Kumar Sardana, Vinay Kumar Agarwal, Wahengbam Romi, Rashmi Kumar, Adarsh Velayudhanpillai, Nitin Bhatheja, Ashok Kumar Raman, Praveen Singh, Virendra Kumar, Sunanda Singhmar, Dinesh Gupta, Shaziya Khan, Damanpreet Singh, Madhav Nilakanth Mugale, Rajat Ujjainiya, Karthik Bharadwaj Tallapaka, Elapavalooru V.S.S.K. Babu, Kiran A Kumar, Rakesh Joshi, Rajeev K. Sukumaran, Amarnarayan D, Sujay Krishna Maity, Dhansekaran Shanmugam, Akanksha Sharma, Akash Kumar Bhaskar, Krishna Khairnar, Gaura Chaturvedi, Amit Lahiri, Vikram Patial, Suresh Kumar Anandasadagopan, Prajeesh Kooloth Valappil, Drishti Soni, Rishabh Jain, Sistla Ramakrishna, Dibyajyoti Ozah, Salwa Naushin, Ravi Kumar Sahu, Geethavani Rayasam, Anurag Agrawal, Sayed G Dastager, and Vandana Singh

Subjects
medicine.medical_specialty, business.industry, Environmental health, Cohort, Epidemiology, Medicine, Phenome, business
Published
2021
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14. Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2

Author

Rajeev K. Sukumaran, Amarnarayan D, Sujay Krishna Maity, Ravindra P Veeranna, Ganesh Venkatachalam, Dayanidhi Singh, Pinreddy Karunakar, Sundaram Acharya, Pawan Gupta, Narendra Vijay Tirpude, Anoop Puthiyamadam, Giriraj R. Chandak, Nitika Kapoor, Babita Sharma, Mahesh Anumalla, Ajinkya Khilari, Dipyaman Ganguly, Prakash M. Halami, Partha Chakrabarti, Birendra Singh Rawat, Gopinath M Sundaram, Shweta Verma, Rakeshkumar Yadav, Purbasha Bhattacharya, Rintu Kutum, Surabhi Seth, Shilpa Paranjape, Sharmistha Sarkar, Viren Sardana, Sunanda Singhmar, Kalpana Chodankar, Mayuri Bhadange, Ajay Agarwal, Ghanshyam Thakkar, Suresh Kumar Anandasadagopan, Debasis Dash, A. Kiran Kumar, Praveen Singh, Debashish Ghosh, Rekha Chouhan, Rajesh P. Ringe, Drishti Soni, Sumit Dahiya, Rohit Yadav, Deepak Sharma, Jasleen Kaur, Yennapu Madhavi, Dibyajyoti Ozah, Satyartha Prakash, Amit Tuli, Syed G. Dastager, Pradip Sen, Arvindkumar H Chaurasiya, Yogendra Padwad, Vipin Hallan, Jit Sarkar, Akanksha Sharma, Samir Damare, Parimala Karuppanan, Anjali Srivastava, Piyush Singh Jamwal, Pankaj Bharali, Tridip Phukan, Amit Lahiri, Bhawna Tomar, Muthukumar Serva Peddha, Amita P Patil, Selvamani Raja Vijayakumar, Salwa Naushin, Sachin N Mahajan, Mahesh J. Kulkarni, Damanpreet Singh, Anil Ku Maurya, Shabda E Kulsange, Virendra Kumar, Akash Kumar Bhaskar, Ravi Kumar Sahu, Krishna Khairnar, Geethavani Rayasam, Prajeesh Kooloth-Valappil, Rashmi Kumar, Madhav Nilakanth Mugale, Shrikant R. Mulay, Susanta Kar, Balthu Narender Kumar, Umakanta Subudhi, Aiswarya R Nair, Anurag Agrawal, Gaura Chaturvedi, Vikram Patial, Sumit G. Gandhi, Abu Junaid Khan, Adarsh Velayudhanpillai, Anupma Thakur, Anirban Pal, Anamika Kothari, Devendra Singh Parihar, Sarita Thakran, Shantanu Sengupta, Shakshi Vashisht, Nitin Bhatheja, Vasudev Wagh, Firdaus Fatima, Prasenjit Manna, Ashok Kumar Raman, Mahesh S. Dharne, Vijay Lakshmi Jamwal, Vandana Singh, Murugan Veerapandian, G. Narahari Sastry, Manikandan Subramanian, Ran Vijay Kumar Singh, Iranna Gogeri, Harish Kumar Sardana, Mrigank Srivastava, Subramanian Venkatesan, Raju Khan, Dhanasekaran Shanmugam, Rahul Chakraborty, Jatin Kalita, Anas Saifi, Sistla Ramakrishna, Rishabh Jain, Saumya Ray Chaudhury, Shaziya Khan, Rajat Ujjainiya, Karthik Bharadwaj Tallapaka, Elapavalooru V.S.S.K. Babu, Avinash Mishra, Wahengbam Romi, Shalini Pradhan, Ved Varun Aggarwal, Dinesh Gupta, Vinay Kumar Agarwal, Akash Pratap Singh, and Rakesh Joshi

Subjects
Male, Time Factors, 030204 cardiovascular system & hematology, Antibodies, Viral, Serology, 0302 clinical medicine, Risk Factors, Seroepidemiologic Studies, Pandemic, Epidemiology, 030212 general & internal medicine, Longitudinal Studies, Biology (General), General Neuroscience, neutralizing antibody, General Medicine, Universal precautions, Cohort, Host-Pathogen Interactions, Medicine, Female, medicine.medical_specialty, 2019-20 coronavirus outbreak, QH301-705.5, Science, India, Lower risk, Risk Assessment, General Biochemistry, Genetics and Molecular Biology, Odds, COVID-19 Serological Testing, 03 medical and health sciences, Predictive Value of Tests, Environmental health, medicine, Humans, Medical history, General Immunology and Microbiology, business.industry, SARS-CoV-2, antibody stability, Industrial research, COVID-19, Odds ratio, Antibodies, Neutralizing, Confidence interval, Immunity, Humoral, sero-prevalence, SARS-CoV2, business, Biomarkers, Demography
Abstract

Background: India has been amongst the most affected nations during the SARS CoV2 pandemic, with sparse data on country wide spread of asymptomatic infections and antibody persistence. This longitudinal cohort study was aimed to evaluate SARS CoV2 seropositivity rate as a marker of infection and evaluate temporal persistence of antibodies with neutralization capability and to infer possible risk factors for infection. Methods: Council of Scientific and Industrial Research, India (CSIR) with its more than 40 laboratories and centers in urban and semi urban settings spread across the country piloted the pan country surveillance. 10427 adult individuals working in CSIR laboratories and their family members based on voluntary participation were assessed for antibody presence and stability was analyzed over 6 months utilizing qualitative Elecysys SARS CoV2 specific antibody kit and GENScript cPass SARS CoV2 Neutralization Antibody Detection Kit. Along with demographic information, possible risk factors were evaluated through self to be filled online forms with data acquired on blood group type, occupation type, addiction and habits including smoking and alcohol, diet preferences, medical history and transport type utilized. Symptom history and information on possible contact and compliance with COVID 19 universal precautions was also obtained. Findings:1058 individuals (10.14%) had antibodies against SARS CoV2. A follow up on 346 seropositive individuals after three months revealed stable to higher antibody levels against SARS CoV2 but declining plasma activity for neutralizing SARS CoV2 receptor binding domain and ACE2 interaction. A repeat sampling of 35 individuals, at six months, revealed declining antibody levels while the neutralizing activity remained stable compared to three months. Majority of seropositive individuals (75%) did not recall even one of nine symptoms since March 2020. Fever was the most common symptom with one fourth reporting loss of taste or smell. Significantly associated risks for seropositivity (Odds Ratio, 95% CI, p value) were observed with usage of public transport (1.79, 1.43 to 2∙24, 2.81561x10-6), occupational responsibilities such as security, housekeeping personnel etc. (2.23, 1.92 to 2.59, 6.43969x10-26), non smokers (1.52, 1.16 to 1.99, 0.02) and non vegetarianism (1.67, 1.41 to 1.99, 3.03821x10-8). An iterative regression analysis was confirmatory and led to only modest changes to estimates. Predilections for seropositivity was noted with specific ABO blood groups; O was associated with a lower risk. Interpretation: In a first of its kind study from India, we report the seropositivity in a country wide cohort and identify variable susceptible associations for contacting infection. Serology and Neutralizing Antibody response provides much sought for general insights on the immune response to the virus among Indians and will be an important resource for designing vaccination strategies. Funding: Council of Scientific and Industrial Research, India (CSIR)

Published
2021

15. Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2

Author

Naushin, Salwa, primary, Sardana, Viren, additional, Ujjainiya, Rajat, additional, Bhatheja, Nitin, additional, Kutum, Rintu, additional, Bhaskar, Akash Kumar, additional, Pradhan, Shalini, additional, Prakash, Satyartha, additional, Khan, Raju, additional, Rawat, Birendra Singh, additional, Tallapaka, Karthik Bharadwaj, additional, Anumalla, Mahesh, additional, Chandak, Giriraj Ratan, additional, Lahiri, Amit, additional, Kar, Susanta, additional, Mulay, Shrikant Ramesh, additional, Mugale, Madhav Nilakanth, additional, Srivastava, Mrigank, additional, Khan, Shaziya, additional, Srivastava, Anjali, additional, Tomar, Bhawana, additional, Veerapandian, Murugan, additional, Venkatachalam, Ganesh, additional, Vijayakumar, Selvamani Raja, additional, Agarwal, Ajay, additional, Gupta, Dinesh, additional, Halami, Prakash M, additional, Peddha, Muthukumar Serva, additional, Sundaram, Gopinath M, additional, Veeranna, Ravindra P, additional, Pal, Anirban, additional, Agarwal, Vinay Kumar, additional, Maurya, Anil Ku, additional, Singh, Ranvijay Kumar, additional, Raman, Ashok Kumar, additional, Anandasadagopan, Suresh Kumar, additional, Karuppanan, Parimala, additional, Venkatesan, Subramanian, additional, Sardana, Harish Kumar, additional, Kothari, Anamika, additional, Jain, Rishabh, additional, Thakur, Anupama, additional, Parihar, Devendra Singh, additional, Saifi, Anas, additional, Kaur, Jasleen, additional, Kumar, Virendra, additional, Mishra, Avinash, additional, Gogeri, Iranna, additional, Rayasam, Geethavani, additional, Singh, Praveen, additional, Chakraborty, Rahul, additional, Chaturvedi, Gaura, additional, Karunakar, Pinreddy, additional, Yadav, Rohit, additional, Singhmar, Sunanda, additional, Singh, Dayanidhi, additional, Sarkar, Sharmistha, additional, Bhattacharya, Purbasha, additional, Acharya, Sundaram, additional, Singh, Vandana, additional, Verma, Shweta, additional, Soni, Drishti, additional, Seth, Surabhi, additional, Vashisht, Sakshi, additional, Thakran, Sarita, additional, Fatima, Firdaus, additional, Singh, Akash Pratap, additional, Sharma, Akanksha, additional, Sharma, Babita, additional, Subramanian, Manikandan, additional, Padwad, Yogendra S, additional, Hallan, Vipin, additional, Patial, Vikram, additional, Singh, Damanpreet, additional, Tripude, Narendra Vijay, additional, Chakrabarti, Partha, additional, Maity, Sujay Krishna, additional, Ganguly, Dipyaman, additional, Sarkar, Jit, additional, Ramakrishna, Sistla, additional, Kumar, Balthu Narender, additional, Kumar, Kiran A, additional, Gandhi, Sumit G, additional, Jamwal, Piyush Singh, additional, Chouhan, Rekha, additional, Jamwal, Vijay Lakshmi, additional, Kapoor, Nitika, additional, Ghosh, Debashish, additional, Thakkar, Ghanshyam, additional, Subudhi, Umakanta, additional, Sen, Pradip, additional, Chaudhury, Saumya Ray, additional, Kumar, Rashmi, additional, Gupta, Pawan, additional, Tuli, Amit, additional, Sharma, Deepak, additional, Ringe, Rajesh P, additional, D, Amarnarayan, additional, Kulkarni, Mahesh, additional, Shanmugam, Dhansekaran, additional, Dharne, Mahesh S, additional, Dastager, Sayed G, additional, Joshi, Rakesh, additional, Patil, Amita P, additional, Mahajan, Sachin N, additional, Khan, Abujunaid Habib, additional, Wagh, Vasudev, additional, Yadav, Rakesh Kumar, additional, Khilari, Ajinkya, additional, Bhadange, Mayuri, additional, Chaurasiya, Arvindkumar H, additional, Kulsange, Shabda E, additional, Khairnar, Krishna, additional, Paranjape, Shilpa, additional, Kalita, Jatin, additional, Sastry, Narahari G, additional, Phukan, Tridip, additional, Manna, Prasenjit, additional, Romi, Wahengbam, additional, Bharali, Pankaj, additional, Ozah, Dibyajyoti, additional, Sahu, Ravi Kumar, additional, Babu, Elapavalooru VSSK, additional, Sukumaran, Rajeev, additional, Nair, Aiswarya R, additional, Valappil, Prajeesh Kooloth, additional, Puthiyamadam, Anoop, additional, Velayudhanpillai, Adarsh, additional, Chodankar, Kalpana, additional, Damare, Samir, additional, Madhavi, Yennapu, additional, Aggarwal, Ved Varun, additional, Dahiya, Sumit, additional, Agrawal, Anurag, additional, Dash, Debasis, additional, and Sengupta, Shantanu, additional

Published
2021
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17. Author response: Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2

Author

Naushin, Salwa, primary, Sardana, Viren, additional, Ujjainiya, Rajat, additional, Bhatheja, Nitin, additional, Kutum, Rintu, additional, Bhaskar, Akash Kumar, additional, Pradhan, Shalini, additional, Prakash, Satyartha, additional, Khan, Raju, additional, Rawat, Birendra Singh, additional, Tallapaka, Karthik Bharadwaj, additional, Anumalla, Mahesh, additional, Chandak, Giriraj Ratan, additional, Lahiri, Amit, additional, Kar, Susanta, additional, Mulay, Shrikant Ramesh, additional, Mugale, Madhav Nilakanth, additional, Srivastava, Mrigank, additional, Khan, Shaziya, additional, Srivastava, Anjali, additional, Tomar, Bhawana, additional, Veerapandian, Murugan, additional, Venkatachalam, Ganesh, additional, Vijayakumar, Selvamani Raja, additional, Agarwal, Ajay, additional, Gupta, Dinesh, additional, Halami, Prakash M, additional, Peddha, Muthukumar Serva, additional, Sundaram, Gopinath M, additional, Veeranna, Ravindra P, additional, Pal, Anirban, additional, Agarwal, Vinay Kumar, additional, Maurya, Anil Ku, additional, Singh, Ranvijay Kumar, additional, Raman, Ashok Kumar, additional, Anandasadagopan, Suresh Kumar, additional, Karuppanan, Parimala, additional, Venkatesan, Subramanian, additional, Sardana, Harish Kumar, additional, Kothari, Anamika, additional, Jain, Rishabh, additional, Thakur, Anupama, additional, Parihar, Devendra Singh, additional, Saifi, Anas, additional, Kaur, Jasleen, additional, Kumar, Virendra, additional, Mishra, Avinash, additional, Gogeri, Iranna, additional, Rayasam, Geethavani, additional, Singh, Praveen, additional, Chakraborty, Rahul, additional, Chaturvedi, Gaura, additional, Karunakar, Pinreddy, additional, Yadav, Rohit, additional, Singhmar, Sunanda, additional, Singh, Dayanidhi, additional, Sarkar, Sharmistha, additional, Bhattacharya, Purbasha, additional, Acharya, Sundaram, additional, Singh, Vandana, additional, Verma, Shweta, additional, Soni, Drishti, additional, Seth, Surabhi, additional, Vashisht, Sakshi, additional, Thakran, Sarita, additional, Fatima, Firdaus, additional, Singh, Akash Pratap, additional, Sharma, Akanksha, additional, Sharma, Babita, additional, Subramanian, Manikandan, additional, Padwad, Yogendra S, additional, Hallan, Vipin, additional, Patial, Vikram, additional, Singh, Damanpreet, additional, Tripude, Narendra Vijay, additional, Chakrabarti, Partha, additional, Maity, Sujay Krishna, additional, Ganguly, Dipyaman, additional, Sarkar, Jit, additional, Ramakrishna, Sistla, additional, Kumar, Balthu Narender, additional, Kumar, Kiran A, additional, Gandhi, Sumit G, additional, Jamwal, Piyush Singh, additional, Chouhan, Rekha, additional, Jamwal, Vijay Lakshmi, additional, Kapoor, Nitika, additional, Ghosh, Debashish, additional, Thakkar, Ghanshyam, additional, Subudhi, Umakanta, additional, Sen, Pradip, additional, Chaudhury, Saumya Ray, additional, Kumar, Rashmi, additional, Gupta, Pawan, additional, Tuli, Amit, additional, Sharma, Deepak, additional, Ringe, Rajesh P, additional, D, Amarnarayan, additional, Kulkarni, Mahesh, additional, Shanmugam, Dhansekaran, additional, Dharne, Mahesh S, additional, Dastager, Sayed G, additional, Joshi, Rakesh, additional, Patil, Amita P, additional, Mahajan, Sachin N, additional, Khan, Abujunaid Habib, additional, Wagh, Vasudev, additional, Yadav, Rakesh Kumar, additional, Khilari, Ajinkya, additional, Bhadange, Mayuri, additional, Chaurasiya, Arvindkumar H, additional, Kulsange, Shabda E, additional, Khairnar, Krishna, additional, Paranjape, Shilpa, additional, Kalita, Jatin, additional, Sastry, Narahari G, additional, Phukan, Tridip, additional, Manna, Prasenjit, additional, Romi, Wahengbam, additional, Bharali, Pankaj, additional, Ozah, Dibyajyoti, additional, Sahu, Ravi Kumar, additional, Babu, Elapavalooru VSSK, additional, Sukumaran, Rajeev, additional, Nair, Aiswarya R, additional, Valappil, Prajeesh Kooloth, additional, Puthiyamadam, Anoop, additional, Velayudhanpillai, Adarsh, additional, Chodankar, Kalpana, additional, Damare, Samir, additional, Madhavi, Yennapu, additional, Aggarwal, Ved Varun, additional, Dahiya, Sumit, additional, Agrawal, Anurag, additional, Dash, Debasis, additional, and Sengupta, Shantanu, additional

Published
2021
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18. Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV-2

Author

Naushin, Salwa, primary, Sardana, Viren, additional, Ujjainiya, Rajat, additional, Bhatheja, Nitin, additional, Kutum, Rintu, additional, Bhaskar, Akash Kumar, additional, Pradhan, Shalini, additional, Prakash, Satyartha, additional, Khan, Raju, additional, Rawat, Birendra Singh, additional, Tallapaka, Karthik Bharadwaj, additional, Anumalla, Mahesh, additional, Chandak, Giriraj Ratan, additional, Lahiri, Amit, additional, Kar, Susanta, additional, Mulay, Shrikant Ramesh, additional, Mugale, Madhav Nilakanth, additional, Srivastava, Mrigank, additional, Khan, Shaziya, additional, Srivastava, Anjali, additional, Tomar, Bhawna, additional, Veerapandian, Murugan, additional, Venkatachalam, Ganesh, additional, Vijayakumar, Selvamani Raja, additional, Agarwal, Ajay, additional, Gupta, Dinesh, additional, Halami, Prakash M, additional, Peddha, Muthukumar Serva, additional, Sundaram, Gopinath M, additional, Veeranna, Ravindra P, additional, Pal, Anirban, additional, Agarwal, Vinay Kumar, additional, Maurya, Anil Ku, additional, Singh, Ran Vijay Kumar, additional, Raman, Ashok Kumar, additional, Anandasadagopan, Suresh Kumar, additional, Karuppanan, Parimala, additional, Venkatesan, Subramanian, additional, Sardana, Harish Kumar, additional, Kothari, Anamika, additional, Jain, Rishabh, additional, Thakur, Anupma, additional, Parihar, Devendra Singh, additional, Saifi, Anas, additional, Kaur, Jasleen, additional, Kumar, Virendra, additional, Mishra, Avinash, additional, Goger, Iranna, additional, Rayasam, Geethavani, additional, Singh, Praveen, additional, Chakraborty, Rahul, additional, Chaturvedi, Gaura, additional, Karunakar, Pinreddy, additional, Yadav, Rohit, additional, Singhmar, Sunanda, additional, Singh, Dayanidhi, additional, Sarkar, Sharmistha, additional, Bhattacharya, Purbasha, additional, Acharya, Sundaram, additional, Singh, Vandana, additional, Verma, Shweta, additional, Soni, Drishti, additional, Seth, Surabhi, additional, Vashisht, Shakshi, additional, Thakran, Sarita, additional, Fatima, Firdaus, additional, Singh, Akash Pratap, additional, Sharma, Akanksha, additional, Sharma, Babita, additional, Subramanian, Manikandan, additional, Padwad, Yogendra, additional, Hallan, Vipin, additional, Patial, Vikram, additional, Singh, Damanpreet, additional, Tirpude, Narendra Vijay, additional, Chakrabarti, Partha, additional, Maity, Sujay Krishna, additional, Ganguly, Dipyaman, additional, Sarkar, Jit, additional, Ramakrishna, Sistla, additional, Kumar, Balthu Narender, additional, Kumar, A Kiran, additional, Gandhi, Sumit G., additional, Jamwal, Piyush Singh, additional, Chouhan, Rekha, additional, Jamwal, Vijay Lakshmi, additional, Kapoor, Nitika, additional, Ghosh, Debashish, additional, Thakkar, Ghanshyam, additional, Subudhi, Umakanta, additional, Sen, Pradip, additional, Chaudhury, Saumya Ray, additional, Kumar, Rashmi, additional, Gupta, Pawan, additional, Tuli, Amit, additional, Sharma, Deepak, additional, Ringe, Rajesh P., additional, D, Amarnarayan, additional, Kulkarni, Mahesh, additional, Shanmugam, Dhanasekaran, additional, Dharne, Mahesh S, additional, Dastager, Syed G., additional, Joshi, Rakesh, additional, Patil, Amita P., additional, Mahajan, Sachin N., additional, Khan, Abu Junaid, additional, Wagh, Vasudev, additional, Yadav, Rakeshkumar, additional, Khilari, Ajinkya, additional, Bhadange, Mayuri, additional, Chaurasiya, Arvindkumar H., additional, Kulsange, Shabda E, additional, Khairnar, Krishna, additional, Paranjape, Shilpa, additional, Kalita, Jatin, additional, Sastry, G. Narahari, additional, Phukan, Tridip, additional, Manna, Prasenjit, additional, Romi, Wahengbam, additional, Bharali, Pankaj, additional, Ozah, Dibyajyoti, additional, Sahu, Ravi Kumar, additional, Babu, Elapavalooru V.S.S.K., additional, Sukumaran, Rajeev, additional, Nair, Aiswarya R, additional, Kooloth-Valappil, Prajeesh, additional, Puthiyamadam, Anoop, additional, Velayudhanpillai, Adarsh, additional, Chodankar, Kalpana, additional, Damare, Samir, additional, Madhavi, Yennapu, additional, Aggarwal, Ved Varun, additional, Dahiya, Sumit, additional, Agrawal, Anurag, additional, Dash, Debasis, additional, and Sengupta, Shantanu, additional

Published
2021
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21. Development of a nitrogen-doped 2D material for tribological applications in the boundary-lubrication regime

Author

Somendra Singh Parihar, Sundara Ramaprabhu, Velayudhanpillai Jayan, and Shende Rashmi Chandrabhan

Subjects
wear, Materials science, Scanning electron microscope, friction, Oxide, General Physics and Astronomy, 02 engineering and technology, lcsh:Chemical technology, lcsh:Technology, Full Research Paper, law.invention, chemistry.chemical_compound, symbols.namesake, 0203 mechanical engineering, X-ray photoelectron spectroscopy, law, nanolubricant, Nanotechnology, General Materials Science, lcsh:TP1-1185, Electrical and Electronic Engineering, Composite material, lcsh:Science, lubrication, Graphene, lcsh:T, Tribology, 021001 nanoscience & nanotechnology, lcsh:QC1-999, Nanoscience, 020303 mechanical engineering & transports, chemistry, Transmission electron microscopy, Lubrication, symbols, tribology, lcsh:Q, 0210 nano-technology, Raman spectroscopy, nitrogen-doped reduced graphene oxide, lcsh:Physics
Abstract

The present paper describes a facile synthesis method for nitrogen-doped reduced graphene oxide (N-rGO) and the application of N-rGO as an effective additive for improving the tribological properties of base oil. N-rGO has been characterized by different characterization techniques such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and Raman spectroscopy. N-rGO-based nanolubricants are prepared and their tribological properties are studied using a four-ball tester. The nanolubricants show excellent stability over a period of six months and a significant decrease in coefficient of friction (25%) for small amounts of N-rGO (3 mg/L). The improvement in tribological properties can be attributed to the sliding mechanism of N-rGO accompanied by the high mechanical strength of graphene. Further, the nanolubricant is prepared at large scale (700 liter) and field trials are carried out at one NTPC thermal plant in India. The implementation of the nanolubricant in an induced draft (ID) fan results in the remarkable decrease in the power consumption.

Published
2017

22. Addressing challenges in production of cellulases for biomass hydrolysis: Targeted interventions into the genetics of cellulase producing fungi

Author

Meena Sankar, Velayudhanpillai-Prasannakumari Adarsh, Rajeev K. Sukumaran, Anoop Puthiyamadam, AthiraRaj Sreeja-Raju, Reshma M. Mathew, Amith Abraham, Valan Rebinro, Aswathi Aswathi, Prajeesh Kooloth-Valappil, Meera Christopher, and Ashok Pandey

Subjects
0106 biological sciences, Environmental Engineering, Catabolite repression, Biomass, Bioengineering, Cellulase, 010501 environmental sciences, Raw material, Lignin, 01 natural sciences, Hydrolysis, 010608 biotechnology, Cellulases, Production (economics), Waste Management and Disposal, 0105 earth and related environmental sciences, chemistry.chemical_classification, biology, Renewable Energy, Sustainability and the Environment, business.industry, Fungi, General Medicine, Biotechnology, Enzyme, chemistry, Biofuel, biology.protein, business
Abstract

Lignocellulosic materials are the favoured feedstock for biorefineries due to their abundant availability and non-completion with food. Biobased technologies for refining these materials are limited mainly by the cost of biomass hydrolyzing enzymes, typically sourced from filamentous fungi. Therefore, considerable efforts have been directed at improving the quantity and quality of secreted lignocellulose degrading enzymes from fungi in order to attain overall economic viability. Process improvements and media engineering probably have reached their thresholds and further production enhancements require modifying the fungal metabolism to improve production and secretion of these enzymes. This review focusses on the types and mechanisms of action of known fungal biomass degrading enzymes, our current understanding of the genetic control exerted on their expression, and possible routes for intervention, especially on modulating catabolite repression, transcriptional regulators, signal transduction, secretion pathways etc., in order to improve enzyme productivity, activity and stability.

Published
2021
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23. Evaluation of a wet processing strategy for mixed phumdi biomass conversion to bioethanol

Author

Kiran Kumar Mallapureddy, Anoop Puthiyamadam, Velayudhanpillai Prasannakumari Adarsh, Thallada Bhaskar, Anil Kuruvilla Mathew, Sudhakara Reddy Yenumala, Jitendra Kumar, Rajeev K. Sukumaran, Dinabandhu Sahoo, and Sabeela Beevi Ummalyama

Subjects
0106 biological sciences, Environmental Engineering, India, Biomass, Bioengineering, 010501 environmental sciences, Raw material, Lignin, 01 natural sciences, Hydrolysate, chemistry.chemical_compound, 010608 biotechnology, Enzymatic hydrolysis, Cellulose, Waste Management and Disposal, 0105 earth and related environmental sciences, Ethanol, Renewable Energy, Sustainability and the Environment, Hydrolysis, General Medicine, Pulp and paper industry, chemistry, Biofuel, Fermentation, Environmental science
Abstract

Biorefineries typically use dry feedstock due to technical and logistic issues, but in unique cases where climatic conditions are unfavorable and where the biomass has to be processed without a holding time, wet processing might be advantageous. The present study evaluated the possibility of using the fresh (non-dried) mixed biomass harvested from Phumdis; which are floating vegetation unique to Loktak lake in Manipur, India, for bioethanol production. Pretreatment with dilute alkali (1.5% at 120 °C for 60 min) resulted in 36% lignin removal and an enhancement of cellulose content to 48% from 37%, and enzymatic hydrolysis released 25 g/L glucose. Fermentation of the hydrolysates was highly efficient at 95%, attained in 36 h and 80% in just 12 h. The new wet processing strategy could help in value addition of mixed phumdi biomass.

Published
2019
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24. Co-pyrolysis of phumdi and para grass biomass from Loktak Lake

Author

Yenumula Sudhakara Reddy, Kiran Kumar Mullepureddy, Ayushi Awasthi, Anoop Puthiyamadam, Sabeela Beevi Ummalyma, Velayudhanpillai Prasannakumari Adarsh, Thallada Bhaskar, Gaje Singh, Jitendra Kumar, Vaibhav Dhyani, Rajeev K. Sukumaran, and Dinabandhu Sahoo

Subjects
0106 biological sciences, Hot Temperature, Environmental Engineering, Biomass, Bioengineering, 010501 environmental sciences, Poaceae, 01 natural sciences, chemistry.chemical_compound, Bioenergy, 010608 biotechnology, Spectroscopy, Fourier Transform Infrared, Phenol, Charcoal, Waste Management and Disposal, 0105 earth and related environmental sciences, Renewable Energy, Sustainability and the Environment, General Medicine, Atmospheric temperature range, Lakes, chemistry, Biofuel, Biofuels, Yield (chemistry), visual_art, visual_art.visual_art_medium, Pyrolysis, Nuclear chemistry
Abstract

In the present work, the pyrolysis of para grass (PG) and phumdi (PH) biomass samples was conducted in the temperature range of 300–500 °C to obtain the optimum temperature for obtaining the maximum yield of bio-oil. Further, co-pyrolysis experiments of PH and PG were also conducted at the same optimized temperature and varied compositions to investigate the synergistic effect. It was observed during the co-pyrolysis, that the maximum bio-oil yield of 37.80 wt% was obtained at the mass ratio of 1:1. The GC–MS, FT-IR and 1H NMR analysis revealed that the bio-oils produced from all the processes were rich in functionalities. Phenolic compounds such as 2-methoxy-4-vinyl phenol, phenol, 2-methoxy, phenol 4-ethyl constituted a significant portion of bio-oils. The biochars obtained at the optimum pyrolytic conditions were analyzed by FT-IR and TOC analyzer.

Published
2019
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