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1. Bioconversion of cellulose into bisabolene using Ruminococcus flavefaciens and Rhodosporidium toruloides

Author

Walls, Laura E, Otoupal, Peter, Ledesma-Amaro, Rodrigo, Velasquez-Orta, Sharon B, Gladden, John M, and Rios-Solis, Leonardo

Subjects
Biological Sciences, Industrial Biotechnology, Cellulose, Ruminococcus, Rhodotorula, Biofuel, Bisabolene, Lignocellulose, Metabollic engineering, Microscale tools, Rhodosporidium toruloides, Rumen bacteria, Ruminococcus flavefaciens, Sequential bioreactors, Biotechnology, Agricultural biotechnology, Industrial biotechnology, Microbiology
Abstract

In this study, organic acids were demonstrated as a promising carbon source for bisabolene production by the non-conventional yeast, Rhodosporidium toruloides, at microscale with a maximum titre of 1055 ± 7 mg/L. A 125-fold scale-up of the optimal process, enhanced bisabolene titres 2.5-fold to 2606 mg/L. Implementation of a pH controlled organic acid feeding strategy at this scale lead to a further threefold improvement in bisabolene titre to 7758 mg/L, the highest reported microbial titre. Finally, a proof-of-concept sequential bioreactor approach was investigated. Firstly, the cellulolytic bacterium Ruminococcus flavefaciens was employed to ferment cellulose, yielding 4.2 g/L of organic acids. R. toruloides was subsequently cultivated in the resulting supernatant, producing 318 ± 22 mg/L of bisabolene. This highlights the feasibility of a sequential bioprocess for the bioconversion of cellulose, into biojet fuel candidates. Future work will focus on enhancing organic acid yields and the use of real lignocellulosic feedstocks to further enhance bisabolene production.

Published
2023

7. Contributors

Author

Acquah, Caleb, primary, Akizuki, Shinichi, additional, Alam, Shahrukh Nawaj, additional, Almeida, Renata Maria Rosas Garcia, additional, Ambati, Ranga Rao, additional, Andreatto Junior, Luis C., additional, Arjun, Ajesh M., additional, Asams, M.A., additional, Aulestia, Denisse Tatiana Molina, additional, Babaie, Meisam, additional, Bastos, Reinaldo G., additional, Bhatia, Pranshu, additional, Bhowmick, Tridib Kumar, additional, Bi, Zheting, additional, Brewer, Catherine E., additional, Cañizares-Villanueva, Rosa Olivia, additional, Celis, Lourdes B., additional, Chatterjee, Pritha, additional, Chen, Ru, additional, Chen, Ying, additional, Cheng, Feng, additional, Chisti, Yusuf, additional, Chuka-Ogwude, David, additional, Codato, Carolina B., additional, Cotas, João, additional, da Motta Pacheco, Luiz Felipe Cetraro, additional, da Silva, Marcio Luis Busi, additional, da Silva, Mariana A., additional, Danquah, Michael K., additional, Das, Abhipsita, additional, Das, Prabir Kumar, additional, de Carvalho, Julio Cesar, additional, de Carvalho, Marco Aurelio, additional, de Farias Silva, Carlos Eduardo, additional, de Jesús Martínez-Roldán, Alfredo, additional, de Lima Araújo, Rosana Reis, additional, de Sá Filho, Márcio Luciano Ferreira, additional, Debnath, Deepayan, additional, Deprá, Mariany Costa, additional, Dias, Rosangela Rodrigues, additional, Ding, Lu, additional, Estrada-Graf, Adrián A., additional, Fathima, Jesna, additional, Ferreira, Ana F., additional, Gajda, Iwona, additional, García-Trejo, Juan Fernando, additional, Gayen, Kalyan, additional, Gerchman, Yoram, additional, Goldemberg, Sérgio, additional, Gómez-Castro, Fernando Israel, additional, Gómez-Ochoa, Manuel, additional, Gonçalves, Ana M.M., additional, Greenman, John, additional, Guieysse, Benoit, additional, Guldhe, Abhishek, additional, Gutiérrez-Antonio, Claudia, additional, Habtu, Nigus Gabbiye, additional, Halim, Ronald, additional, Harris, Jonathan S., additional, Harvey, Adam P., additional, He, Bingjun Brian, additional, Henderson, Rita K., additional, Hernández, Salvador, additional, Ieropoulos, Ioannis A., additional, Jacob-Furlan, Beatriz, additional, Jacob-Lopes, Eduardo, additional, Jeevanandam, Jaison, additional, Kafarov, Vyacheslav, additional, Karytsas, Spyridon, additional, Khalid, Zaira, additional, Kiani, Hossein, additional, Kishi, Masatoshi, additional, Kong, Fantao, additional, Kosowska-Golachowska, Monika, additional, Kumar, Sanjay, additional, Lasta, Paola, additional, Legesse, Solomon Addisu, additional, Li, Yuchen, additional, Liu, Keqing, additional, Luckos, Adam, additional, Magalhães, Antônio Irineudo, additional, Mariano, André B., additional, Maroneze, Mariana Manzoni, additional, Mattos, Luis Felipe A., additional, Memon, Asif Ali, additional, Mendis, Arjuna, additional, Menezes, Mariana, additional, Michelon, William, additional, Mohiuddin, Obaidullah, additional, Morales-Ibarría, Marcia, additional, Muñoz, Raúl, additional, Nunes, Moira, additional, Nwoba, Emeka G., additional, Ofori-Boateng, Cynthia, additional, Ogbonna, James C., additional, Ojeda, Karina, additional, Onay, Melih, additional, Ong, Hui Lin, additional, Orta Ledesma, Ma. Teresa, additional, Pacheco, Diana, additional, Paravantis, John A., additional, Perazzoli, Simone, additional, Pereira, Leonel, additional, Phan, Anh N., additional, Pinheiro, Pricila Nass, additional, Plouviez, Maxence, additional, Raheem, Abdul, additional, Rao, Narasinga Rao Hanumanth, additional, Ravishankar Gokare, A., additional, Raya, Rosario Rodero, additional, Razo-Flores, Elías, additional, Reijnders, Lucas, additional, Rimmer, Matthew, additional, Rincón, Bárbara, additional, Rincón-Pérez, Jack, additional, Rivas, Mariella, additional, Romero-Izquierdo, Araceli Guadalupe, additional, Rossi, Raquel, additional, Ruiz-Ruiz, Patricia, additional, Sajid, Muhammad, additional, Sánchez-Tuirán, Eduardo Luis, additional, Sarkar, Sambit, additional, Sartori, Rafaela Basso, additional, Sen, Suparna, additional, Severo, Ihana Aguiar, additional, Singh, Bhaskar, additional, Soccol, Carlos Ricardo, additional, Suzuki, Seiiti, additional, Sydney, Eduardo Bittencourt, additional, Tang, Ying, additional, Tiwari, Brijesh K., additional, Vadiveloo, Ashiwin, additional, Vargas, José Viriato C., additional, Velasquez-Orta, Sharon B., additional, Vieira, Karem Rodrigues, additional, Vigani, Mauro, additional, Villagracia, Al Rey, additional, Walter, Xavier Alexis, additional, Wood, David A., additional, Yang, Rui, additional, You, Jiseon, additional, Yu, Guangsuo, additional, Zepka, Leila Queiroz, additional, and Zhu, Chenba, additional

Published
2022
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17. Additional file 1 of Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae

Author

Romero-Frasca, Enrique, Velasquez-Orta, Sharon B., Escobar-Sánchez, Viviana, Raunel Tinoco-Valencia, and Ledesma, María Teresa Orta

Abstract

Additional file 1. Figure S1. Design matrix with coded levels and real values (in parenthesis) for a three-level full factorial design. Figure S2. Calibration curve for tested parameters in HPLC using laboratory grade reagents. Figure S3. Calibration curve for ethanol quantification in GC using laboratory grade reagents. Table S1. High-Performance Liquid Chromatographer instruments and chromatographic conditions. Table S2. Gas Chromatographer instruments and chromatographic conditions.

Published
2021
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30. Microbial Electrosynthesis and Anaerobic Fermentation: An Economic Evaluation for Acetic Acid Production from CO2 and CO.

Author

Xenia Christodoulou and Velasquez-Orta, Sharon B.

Subjects
*ELECTROSYNTHESIS, *ACETIC acid, *GREENHOUSE gases, *INDUSTRIAL costs, *BIOTECHNOLOGICAL process monitoring, *CHEMICAL reactions, *CARBON monoxide
Abstract

Microbial electrosynthesis (MES) and anaerobic fermentation (AF) are two biological processes capable of reducing CO2, CO, and water into acetic acid, an essential industrial reagent. In this study, we evaluated investment and production costs of acetic acid via MES and AF, and compared them to industrial chemical processes: methanol carbonylation and ethane direct oxidation. Production and investment costs were found high-priced for MES (1.44 £/kg, 1770 £/t) and AF (4.14 £/kg, 1598 £/t) because of variable and fixed costs and low production yields (100 t/y) compared to methanol carbonylation (0.26 £/kg, 261 £/t) and ethane direct oxidation (0.11 £/kg, 258 £/t). However, integrating AF with MES would reduce the release of CO2, double production rates (200 t/y), and decrease investment costs by 9% (1366 £/t). This resulted into setting the production costs at 0.24 £/kg which is currently market competitive (0.48 £/kg). This economically feasible bioprocess produced molar flow rates of 4550 mol per day from MES and AF independently. Our findings offer a bright opportunity toward the use and scale-up of MES and AF for an economically viable acetic acid production process. [ABSTRACT FROM AUTHOR]

Published
2016
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34. Microbial Electrosynthesis and Anaerobic Fermentation: An Economic Evaluation for Acetic Acid Production from CO 2 and CO.

Author

Christodoulou X and Velasquez-Orta SB

Subjects
Acetic Acid, Anaerobiosis, Bacteria chemistry, Cost-Benefit Analysis, Carbon Dioxide chemistry, Carbon Monoxide chemistry, Fermentation
Abstract

Microbial electrosynthesis (MES) and anaerobic fermentation (AF) are two biological processes capable of reducing CO 2 , CO, and water into acetic acid, an essential industrial reagent. In this study, we evaluated investment and production costs of acetic acid via MES and AF, and compared them to industrial chemical processes: methanol carbonylation and ethane direct oxidation. Production and investment costs were found high-priced for MES (1.44 £/kg, 1770 £/t) and AF (4.14 £/kg, 1598 £/t) because of variable and fixed costs and low production yields (100 t/y) compared to methanol carbonylation (0.26 £/kg, 261 £/t) and ethane direct oxidation (0.11 £/kg, 258 £/t). However, integrating AF with MES would reduce the release of CO 2 , double production rates (200 t/y), and decrease investment costs by 9% (1366 £/t). This resulted into setting the production costs at 0.24 £/kg which is currently market competitive (0.48 £/kg). This economically feasible bioprocess produced molar flow rates of 4550 mol per day from MES and AF independently. Our findings offer a bright opportunity toward the use and scale-up of MES and AF for an economically viable acetic acid production process.

Published
2016
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