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195 results on '"Vegesna, A. K."'

1. Aldehydes alter TGF-β signaling and induce obesity and cancer

Author

Yang, Xiaochun, Bhowmick, Krishanu, Rao, Shuyun, Xiang, Xiyan, Ohshiro, Kazufumi, Amdur, Richard L., Hassan, Md. Imtaiyaz, Mohammad, Taj, Crandall, Keith, Cifani, Paolo, Shetty, Kirti, Lyons, Scott K., Merrill, Joseph R., Vegesna, Anil K., John, Sahara, Latham, Patricia S., Crawford, James M., Mishra, Bibhuti, Dasarathy, Srinivasan, Wang, Xin Wei, Yu, Herbert, Wang, Zhanwei, Huang, Hai, Krainer, Adrian R., and Mishra, Lopa

Published
2024
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2. Thiophene bridged aldehydes (TBAs) image ALDH activity in cells via modulation of intramolecular charge transfer.

Author

Maity, Santanu, Sadlowski, Corinne M, George Lin, Jung-Ming, Chen, Che-Hong, Peng, Li-Hua, Lee, Eun-Soo, Vegesna, Giri K, Lee, Charles, Kim, Se-Hwa, Mochly-Rosen, Daria, Kumar, Sanjay, and Murthy, Niren

Subjects
Chemical Sciences
Abstract

Aldehyde dehydrogenases (ALDHs) catalyze the oxidation of an aldehyde to a carboxylic acid and are implicated in the etiology of numerous diseases. However, despite their importance, imaging ALDH activity in cells is challenging due to a lack of fluorescent imaging probes. In this report, we present a new family of fluorescent probes composed of an oligothiophene flanked by an aldehyde and an electron donor, termed thiophene-bridged aldehydes (TBAs), which can image ALDH activity in cells. The TBAs image ALDH activity via a fluorescence sensing mechanism based on the modulation of intramolecular charge transfer (ICT) and this enables the TBAs and their ALDH-mediated oxidized products, thiophene-bridged carboxylates (TBCs), to have distinguishable fluorescence spectra. Herein, we show that the TBAs can image ALDH activity in cells via fluorescence microscopy, flow cytometry, and in a plate reader. Using TBA we were able to develop a cell-based high throughput assay for ALDH inhibitors, for the first time, and screened a large, 1460-entry electrophile library against A549 cells. We identified α,β-substituted acrylamides as potent electrophile fragments that can inhibit ALDH activity in cells. These inhibitors sensitized drug-resistant glioblastoma cells to the FDA approved anti-cancer drug, temozolomide. The TBAs have the potential to make the analysis of ALDH activity in cells routinely possible given their ability to spectrally distinguish between an aldehyde and a carboxylic acid.

Published
2017

3. Incremental yield of dysplasia detection in Barrett’s esophagus using volumetric laser endomicroscopy with and without laser marking compared with a standardized random biopsy protocol

Author

Alshelleh, Mohammad, Inamdar, Sumant, McKinley, Matthew, Stewart, Molly, Novak, Jeffrey S., Greenberg, Ronald E., Sultan, Keith, Devito, Bethany, Cheung, Mary, Cerulli, Maurice A., Miller, Larry S., Sejpal, Divyesh V., Vegesna, Anil K., and Trindade, Arvind J.

Published
2018
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4. Impaired reciprocal regulation between SIRT6 and TGF‐β signaling in fatty liver

Author

Xiang, Xiyan, primary, Ohshiro, Kazufumi, additional, Zaidi, Sobia, additional, Yang, Xiaochun, additional, Bhowmick, Krishanu, additional, Vegesna, Anil K., additional, Bernstein, David, additional, Crawford, James M., additional, Mishra, Bibhuti, additional, Latham, Patricia S., additional, Gough, Nancy R., additional, Rao, Shuyun, additional, and Mishra, Lopa, additional

Published
2022
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7. Comparison of human and porcine gastric clasp and sling fiber contraction by [M.sub.2] and [M.sub.3] muscarinic receptors

Author

Vegesna, Anil K., Braverman, Alan S., Miller, Larry S., Tallarida, Ronald J., Tiwana, Mansoor I., Khayyam, Umar, and Ruggieri, Michael R., Sr.

Subjects
Swine -- Comparative analysis, Swine -- Health aspects, Gastroesophageal reflux -- Research, Gastroesophageal reflux -- Comparative analysis, Human beings -- Health aspects, Human beings -- Comparative analysis, Man -- Health aspects, Man -- Comparative analysis, Biological sciences
Abstract

To compare gastroesophageal junction of the human with the pig, [M.sub.2] and [M.sub.3] receptor densities and the potencies of [M.sub.2] and [M.sub.3] muscarinic receptor subtype selective antagonists were determined in gastric clasp and sling smooth muscle fibers. Total muscarinic and [M.sub.2] receptors are higher in pig than human clasp and sling fibers. [M.sub.3] receptors are higher in human compared with pig sling fibers but lower in human compared with pig clasp fibers. Clasp fibers have fewer [M.sub.3] receptors than sling fibers in both humans and pigs. Similar to human clasp fibers, pig clasp fibers contract significantly less than pig sling fibers. Analysis of the methoctramine Schild plot suggests that [M.sub.2] receptors are involved in mediating contraction in pig clasp and sling fibers. Darifenacin potency suggests that [M.sub.3] receptors mediate contraction in pig sling fibers and that [M.sub.2] and [M.sub.3] receptors mediate contraction in pig clasp fibers. Taken together, the data suggest that both [M.sub.2] and [M.sub.3] muscarinic receptors mediate the contraction in both pig clasp and sling fibers similar to human clasp and sling fibers. doi: 10.1152/ajpi.00129.2009 gastroesophageal reflux; receptor immunoprecipitation; smooth muscle contractility; animal models

Published
2010

25. Tu1136 Reflex Increase Within the Gastroesophageal Junction High Pressure Zone (GEJHPZ) During Sedated Endoscopy and Decrease During Unsedated Endoscopy With Gastric Distension in Normal Volunteers

Author

Vegesna, Anil K., primary, Sachdeva, Priyanka, additional, Makipour, Kian, additional, Murari, Pallavi, additional, Prabhu, Shital P., additional, Sampath, Aiswerya Madanam, additional, Farooq, Muhammad A., additional, and Miller, Larry S., additional

Published
2012
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36. Measurement of Ileal Cecal Valve Pressures

Author

Vegesna, Anil K., primary, Prabhu, Shital P., additional, Madanam Sampath, Aiswerya, additional, Yarar, Dilek, additional, Ramzan, Zeeshan, additional, Makipour, Kian, additional, and Miller, Larry S., additional

Published
2011
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