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3. Evaluating vector competence for Yellow fever in the Caribbean

Author

Gabiane, Gaelle, Bohers, Chloé, Mousson, Laurence, Obadia, Thomas, Dinglasan, Rhoel R., Vazeille, Marie, Dauga, Catherine, Viglietta, Marine, Yébakima, André, Vega-Rúa, Anubis, Gutiérrez Bugallo, Gladys, Gélvez Ramírez, Rosa Margarita, Sonor, Fabrice, Etienne, Manuel, Duclovel-Pame, Nathalie, Blateau, Alain, Smith-Ravin, Juliette, De Lamballerie, Xavier, and Failloux, Anna-Bella

Published
2024
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6. Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

Author

Hery, Lyza, Guidez, Amandine, Durand, Audrey-Anne, Delannay, Christelle, Normandeau-Guimond, Josiann, Reynaud, Yann, Issaly, Jean, Goindin, Daniella, Legrave, Grégory, Gustave, Joel, Raffestin, Stéphanie, Breurec, Sebastien, Constant, Philippe, Dusfour, Isabelle, Guertin, Claude, and Vega-Rúa, Anubis

Published
2021
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7. Extensive variation and strain-specificity in dengue virus susceptibility among AfricanAedes aegyptipopulations

Author

Dabo, Stéphanie, primary, Henrion-Lacritick, Annabelle, additional, Lecuyer, Alicia, additional, Jiolle, Davy, additional, Paupy, Christophe, additional, Ayala, Diego, additional, da Veiga Leal, Silvânia, additional, Badolo, Athanase, additional, Vega-Rúa, Anubis, additional, Sylla, Massamba, additional, Akorli, Jewelna, additional, Otoo, Sampson, additional, Lutomiah, Joel, additional, Sang, Rosemary, additional, Mutebi, John-Paul, additional, Saleh, Maria-Carla, additional, Rose, Noah H., additional, McBride, Carolyn S., additional, and Lambrechts, Louis, additional

Published
2023
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8. Extensive variation and strain-specificity in dengue virus susceptibility among African Aedes aegypti populations.

Author

Dabo, Stéphanie, Henrion-Lacritick, Annabelle, Lecuyer, Alicia, Jiolle, Davy, Paupy, Christophe, Ayala, Diego, da Veiga Leal, Silvânia, Badolo, Athanase, Vega-Rúa, Anubis, Sylla, Massamba, Akorli, Jewelna, Otoo, Sampson, Lutomiah, Joel, Sang, Rosemary, Mutebi, John-Paul, Saleh, Maria-Carla, Rose, Noah H., McBride, Carolyn S., and Lambrechts, Louis

Subjects
DENGUE hemorrhagic fever, AEDES aegypti, DENGUE viruses, ZIKA virus, VIRUS diversity, AFRICANS, MOSQUITO vectors, SEROTYPES
Abstract

African populations of the mosquito Aedes aegypti are usually considered less susceptible to infection by human-pathogenic flaviviruses than globally invasive populations found outside Africa. Although this contrast has been well documented for Zika virus (ZIKV), it is unclear to what extent it is true for dengue virus (DENV), the most prevalent flavivirus of humans. Addressing this question is complicated by substantial genetic diversity among DENV strains, most notably in the form of four genetic types (DENV1 to DENV4), that can lead to genetically specific interactions with mosquito populations. Here, we carried out a survey of DENV susceptibility using a panel of seven field-derived Ae. aegypti colonies from across the African range of the species and a colony from Guadeloupe, French West Indies as non-African reference. We found considerable variation in the ability of African Ae. aegypti populations to acquire and replicate a panel of six DENV strains spanning the four DENV types. Although African Ae. aegypti populations were generally less susceptible than the reference non-African population from Guadeloupe, in several instances some African populations were equally or more susceptible than the Guadeloupe population. Moreover, the relative level of susceptibility between African mosquito populations depended on the DENV strain, indicating genetically specific interactions. We conclude that unlike ZIKV susceptibility, there is no clear-cut dichotomy in DENV susceptibility between African and non-African Ae. aegypti. DENV susceptibility of African Ae. aegypti populations is highly heterogeneous and largely governed by the specific pairing of mosquito population and DENV strain. Author summary: African populations of the mosquito Aedes aegypti are usually thought to be less likely to get infected by flaviviruses compared to Ae. aegypti mosquitoes found outside Africa. While this has been well-demonstrated for Zika virus, it is not clear if the same is true for dengue virus, which is the most common flavivirus in humans. Studying this is complicated by the strain diversity of dengue virus, including four main genetic types, potentially causing different interactions. In this study, we compared several mosquito populations and found that, in general, African mosquitoes were less likely to get infected by dengue virus compared to mosquitoes from outside Africa. However, in some cases, African mosquitoes were just as or even more likely to get infected. The specific strain of dengue virus also influenced how likely African mosquitoes were to get infected, showing that the relationship between African mosquitoes and dengue virus is complex. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Estimating the risk of Yellow fever in the Caribbean using vector competence data

Author

FAILLOUX, Anna-Bella, primary, GABIANE, Gaelle, additional, MOUSSON, Laurence, additional, BOHERS, Chloé, additional, Obadia, Thomas, additional, Dinglasan, Rhoel, additional, VAZEILLE, Marie, additional, DAUGA, Catherine, additional, VIGLIETTA, Marine, additional, YEBAKIMA, André, additional, Vega-Rúa, Anubis, additional, GUTIERREZ-BUGALLO, Gladys, additional, RAMIREZ, Rosa Margarita GELVEZ, additional, SONOR, Fabrice, additional, ETIENNE, Manuel, additional, DUCLOVEL-PAME, Nathalie, additional, BLATEAU, Alain, additional, SMITH-RAVIN, Juliette, additional, and de Lamballerie, Xavier, additional

Published
2023
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10. Phylogenetic Investigations of Dengue 2019–2021 Outbreak in Guadeloupe and Martinique Caribbean Islands

Author

Garcia--Van Smévoorde, Margot, primary, Piorkowski, Géraldine, additional, Emboulé, Loic, additional, Dos Santos, Georges, additional, Loraux, Cécile, additional, Guyomard-Rabenirina, Stéphanie, additional, Joannes, Marie-Odile, additional, Fagour, Laurence, additional, Najioullah, Fatiha, additional, Cabié, André, additional, de Lamballerie, Xavier, additional, Vega-Rúa, Anubis, additional, Césaire, Raymond, additional, and Calvez, Elodie, additional

Published
2023
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15. First report of natural Wolbachia infections in mosquitoes from Cuba

Author

Ruiz, Armando, primary, Gutiérrez-Bugallo, Gladys, additional, Rodríguez-Roche, Rosmari, additional, Pérez, Lissette, additional, González-Broche, Raúl, additional, Piedra, Luis A., additional, Martínez, Liss C., additional, Menéndez, Zulema, additional, Vega-Rúa, Anubis, additional, and Bisset, Juan A., additional

Published
2023
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19. Bidirectional Interactions between Arboviruses and the Bacterial and Viral Microbiota in Aedes aegypti and Culex quinquefasciatus

Author

Shi, Chenyan, primary, Beller, Leen, additional, Wang, Lanjiao, additional, Rosales Rosas, Ana, additional, De Coninck, Lander, additional, Héry, Lyza, additional, Mousson, Laurence, additional, Pagès, Nonito, additional, Raes, Jeroen, additional, Delang, Leen, additional, Vega-Rúa, Anubis, additional, Failloux, Anna-bella, additional, and Matthijnssens, Jelle, additional

Published
2022
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22. Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project

Author

Obadia, Thomas, primary, GUTIERREZ-BUGALLO, Gladys, additional, Duong, Veasna, additional, NUNEZ, Ana, additional, FERNANDES, Rosilainy, additional, Kamgang, Basile, additional, Héry, Lyza, additional, GOMARD, Yann, additional, ABBO, Sandra, additional, JIOLLE, Davy, additional, GLAVINIC, Uros, additional, DUPONT-ROUZEYROL, Myrielle, additional, Atyame, Célestine, additional, POCQUET, Nicolas, additional, BOYER, Sébastien, additional, DAUGA, Catherine, additional, VAZEILLE, Marie, additional, YEBAKIMA, André, additional, White, Michael, additional, Koenraadt, Constantianus, additional, MAVINGUI, Patrick, additional, Vega-Rúa, Anubis, additional, VERONESI, Eva, additional, Pijlman, Gorben, additional, PAUPY, Christophe, additional, BUSQUETS, Nuria, additional, Lourenço-de-Oliveira, Ricardo, additional, de Lamballerie, Xavier, additional, and FAILLOUX, Anna-Bella, additional

Published
2022
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23. Bidirectional Interactions between Arboviruses and the Bacterial and Viral Microbiota in Aedes aegypti and Culex quinquefasciatus

Author

Shi, Chenyan, Beller, Leen, Wang, Lanjiao, Rosales Rosas, Ana, De Coninck, Lander, Héry, Lyza, Mousson, Laurence, Pagès, Nonito, Raes, Jeroen, Delang, Leen, Vega-Rúa, Anubis, Failloux, Anna-Bella, Matthijnssens, Jelle, Shi, Chenyan, Beller, Leen, Wang, Lanjiao, Rosales Rosas, Ana, De Coninck, Lander, Héry, Lyza, Mousson, Laurence, Pagès, Nonito, Raes, Jeroen, Delang, Leen, Vega-Rúa, Anubis, Failloux, Anna-Bella, and Matthijnssens, Jelle

Abstract

Mosquitoes are important vectors for many arboviruses. It is becoming increasingly clear that various symbiotic microorganisms (including bacteria and insect-specific viruses; ISVs) in mosquitoes have the potential to modulate the ability of mosquitoes to transmit arboviruses. In this study, we compared the bacteriome and virome (both eukaryotic viruses and bacteriophages) of female adult Aedes aegypti and Culex quinquefasciatus mosquitoes fed with sucrose/water, blood, or blood spiked with Zika virus (ZIKV) or West Nile virus (WNV), respectively. Furthermore, we investigated associations between the microbiota and vector competence. We show that the influence of arboviruses on the mosquito microbiome—and vice versa—is distinct for each combination of arbovirus/mosquito species. The presence of ZIKV resulted in a temporarily increased Aedes ISV diversity. However, this effect was distinct for different ISVs: some ISVs decreased following the blood meal (Aedes aegypti totivirus), whereas other ISVs increased only when the blood contained ZIKV (Guadeloupe mosquito virus). Also, the diversity of the Aedes bacteriome depended on the diet and the presence of ZIKV, with a lower diversity observed for mosquitoes receiving blood without ZIKV. In Cx. quinquefasciatus, some ISVs increased in WNV-infected mosquitoes (Guadeloupe Culex tymo-like virus). Particularly, the presence of Wenzhou sobemo-like virus 3 (WSLV3) was associated with the absence of infectious WNV in mosquito heads, suggesting that WSLV3 might affect vector competence for WNV. Distinct profiles of bacteriophages were identified in Culex mosquitoes depending on diet, despite the lack of clear changes in the bacteriome. Overall, our data demonstrate a complex three-way interaction among arboviruses, resident microbiota, and the host, which is distinct for different arbovirus–mosquito combinations. A better understanding of these interactions may lead to the identification of microbiota able to suppress the abil

Published
2022

24. Critical Evaluation of Cross-Sectoral Collaborations to Inform the Implementation of the 'One Health' Approach in Guadeloupe

Author

Gruel, Gaëlle, Diouf, Mame Boucar, Abadie, Catherine, Chilin-Charles, Yolande, Etter, Eric, Geffroy, Mariana, Herrmann Storck, Cécile, Meyer, Damien, Pagès, Nonito, Pressat, Gersende, Teycheney, Pierre-Yves, Umber, Marie, Vega-Rúa, Anubis, Pradel, Jennifer, Unité Transmission, Réservoir et Diversité des Pathogènes [Pasteur Guadeloupe, France] (TReD-Path), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Agrosystèmes tropicaux (ASTRO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), CHU Pointe-à-Pitre/Abymes [Guadeloupe], MALIN project funded by the European Union and the Guadeloupe Regional Council under the European Research, Development Funds (ERDF) 2014-2020 program 2018-FED-1084, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Plant Health Institute of Montpellier (UMR PHIM), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro

Subjects
H01 - Protection des végétaux - Considérations générales, interdisciplinary and cross-sectoral collaborations, Natural Disasters, West Indies, Politique sanitaire, Recherche interdisciplinaire, environmental health, human health, Santé publique, santé des plantes, Animals, Humans, Surveillance épidémiologique, One Health, Guadeloupe, evaluation, animal health, Santé animale, L70 - Sciences et hygiène vétérinaires - Considérations générales, collaboration, Protection de l'environnement, Caribbean Region, S50 - Santé humaine, operationalization, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Public Health, plant health, Community Case Study
Abstract

International audience; In Guadeloupe, a French overseas territory located in the Eastern Caribbean, infectious and non-infectious diseases, loss of biodiversity, natural disasters and global change threaten the health and well-being of animals, plants, and people. Implementing the “One Health” (OH) approach is crucial to reduce the archipelago's vulnerability to these health threats. However, OH remains underdeveloped in Guadeloupe, hampering efficient and effective intersectoral and transdisciplinary collaborations for disease surveillance and control. A multidisciplinary research group of volunteer researchers working in Guadeloupe, with collective expertise in infectious diseases, undertook a study to identify key attributes for OH operationalization by reviewing past and current local collaborative health initiatives and analyzing how much they mobilized the OH framework. The research group developed and applied an operational OH framework to assess critically collaborative initiatives addressing local health issues. Based on a literature review, a set of 13 opinion-based key criteria was defined. The criteria and associated scoring were measured through semi-directed interviews guided by a questionnaire to critically evaluate four initiatives in animal, human, plant, and environmental health research and epidemiological surveillance. Gaps, levers, and prospects were identified that will help health communities in Guadeloupe envision how to implement the OH approach to better address local health challenges. The methodology is simple, generic, and pragmatic and relies on existing resources. It can be transposed and adapted to other contexts to improve effectiveness and efficiency of OH initiatives, based on lessons-learned of local past or current multi-interdisciplinary and intersectoral initiatives.

Published
2021

25. Quantification of breeding sites parameters in shaping bacterial communities in Aedes aegypti (Diptera: Culicidae)

Author

Normandeau-Guimond, Josiann, primary, Hery, Lyza, additional, Guidez, Amandine, additional, Durand, Audrey-Anne, additional, Delannay, Christelle, additional, Issaly, Jean, additional, Raffestin, Stéphanie, additional, Nigro, Joseph, additional, Vega-Rúa, Anubis, additional, Constant, Philippe, additional, Guertin, Claude, additional, and Dusfour, Isabelle, additional

Published
2021
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26. Shifting From Sectoral to Integrated Surveillance by Changing Collaborative Practices: Application to West Nile Virus Surveillance in a Small Island State of the Caribbean

Author

Geffroy, Mariana, primary, Pagès, Nonito, additional, Chavernac, David, additional, Dereeper, Alexis, additional, Aubert, Lydéric, additional, Herrmann-Storck, Cecile, additional, Vega-Rúa, Anubis, additional, Lecollinet, Sylvie, additional, and Pradel, Jennifer, additional

Published
2021
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27. Shifting from sectoral to integrated surveillance by changing collaborative practices: Application to west nile virus surveillance in a small Island State of the Caribbean

Author

Geffroy, Mariana, Pagès, Nonito, Chavernac, David, Dereeper, Alexis, Aubert, Lydéric, Herrmann-Storck, Cecile, Vega-Rúa, Anubis, Lecollinet, Sylvie, Pradel, Jennifer, Geffroy, Mariana, Pagès, Nonito, Chavernac, David, Dereeper, Alexis, Aubert, Lydéric, Herrmann-Storck, Cecile, Vega-Rúa, Anubis, Lecollinet, Sylvie, and Pradel, Jennifer

Abstract

After spreading in the Americas, West Nile virus was detected in Guadeloupe (French West Indies) for the first time in 2002. Ever since, several organizations have conducted research, serological surveys, and surveillance activities to detect the virus in horses, birds, mosquitoes, and humans. Organizations often carried them out independently, leading to knowledge gaps within the current virus' situation. Nearly 20 years after the first evidence of West Nile virus in the archipelago, it has not yet been isolated, its impact on human and animal populations is unknown, and its local epidemiological cycle is still poorly understood. Within the framework of a pilot project started in Guadeloupe in 2019, West Nile virus was chosen as a federative model to apply the “One Health” approach for zoonotic epidemiological surveillance and shift from a sectorial to an integrated surveillance system. Human, animal, and environmental health actors involved in both research and surveillance were considered. Semi-directed interviews and a Social Network Analysis were carried out to learn about the surveillance network structure and actors, analyze information flows, and identify communication challenges. An information system was developed to fill major gaps: users' needs and main functionalities were defined through a participatory process where actors also tested and validated the tool. Additionally, all actors shared their data, which were digitized, cataloged, and centralized, to be analyzed later. An R Shiny server was integrated into the information system, allowing an accessible and dynamic display of data showcasing all of the partners' information. Finally, a series of virtual workshops were organized among actors to discuss preliminary results and plan the next steps to improve West Nile Virus and vector-borne or emerging zoonosis surveillance. The actors are willing to build a more resilient and cooperative network in Guadeloupe with improved relevance, efficiency, and e

Published
2021

31. Enhanced Zika virus susceptibility of globally invasive Aedes aegypti populations

Author

Aubry, Fabien, primary, Dabo, Stéphanie, additional, Manet, Caroline, additional, Filipović, Igor, additional, Rose, Noah H., additional, Miot, Elliott F., additional, Martynow, Daria, additional, Baidaliuk, Artem, additional, Merkling, Sarah H., additional, Dickson, Laura B., additional, Crist, Anna B., additional, Anyango, Victor O., additional, Romero-Vivas, Claudia M., additional, Vega-Rúa, Anubis, additional, Dusfour, Isabelle, additional, Jiolle, Davy, additional, Paupy, Christophe, additional, Mayanja, Martin N., additional, Lutwama, Julius J., additional, Kohl, Alain, additional, Duong, Veasna, additional, Ponlawat, Alongkot, additional, Sylla, Massamba, additional, Akorli, Jewelna, additional, Otoo, Sampson, additional, Lutomiah, Joel, additional, Sang, Rosemary, additional, Mutebi, John-Paul, additional, Cao-Lormeau, Van-Mai, additional, Jarman, Richard G., additional, Diagne, Cheikh T., additional, Faye, Oumar, additional, Faye, Ousmane, additional, Sall, Amadou A., additional, McBride, Carolyn S., additional, Montagutelli, Xavier, additional, Rašić, Gordana, additional, and Lambrechts, Louis, additional

Published
2020
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32. Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana

Author

Hery, Lyza, primary, Guidez, Amandine, additional, Durand, Audrey-Anne, additional, Delannay, Christelle, additional, Normandeau-Guimond, Josiann, additional, Reynaud, Yann, additional, Issaly, Jean, additional, Goindin, Daniella, additional, Legrave, Grégory, additional, Gustave, Joel, additional, Raffestin, Stéphanie, additional, Breurec, Sebastien, additional, Constant, Philippe, additional, Dusfour, Isabelle, additional, Guertin, Claude, additional, and Vega-Rúa, Anubis, additional

Published
2020
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33. Zika virus polyprotein gene, complete cds

Author

Moutailler, Sara, Yousfi, Lena, Mousson, Laurence, Devillers, Elodie, Vazeille, Marie, Vega-Rúa, Anubis, Perrin, Yvon, Jourdain, Frédéric, Chandre, Fabrice, Cannet, Arnaud, Chantilly, Sandrine, Restrepo, Johana, Guidez, Amandine, Dusfour, Isabelle, Vieira Santos de Abreu, Filipe, Pereira Dos Santos, Taissa, Jiolle, Davy, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril, Diallo, Mawlouth, Diallo, Diawo, Gaye, Alioune, Boyer, Sébastien, Duong, Veasna, Piorkowski, Géraldine, Paupy, Christophe, Lourenco de Oliveira, Ricardo, de Lamballerie, Xavier, Failloux, Anna Bella, Moutailler, Sara, Yousfi, Lena, Mousson, Laurence, Devillers, Elodie, Vazeille, Marie, Vega-Rúa, Anubis, Perrin, Yvon, Jourdain, Frédéric, Chandre, Fabrice, Cannet, Arnaud, Chantilly, Sandrine, Restrepo, Johana, Guidez, Amandine, Dusfour, Isabelle, Vieira Santos de Abreu, Filipe, Pereira Dos Santos, Taissa, Jiolle, Davy, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril, Diallo, Mawlouth, Diallo, Diawo, Gaye, Alioune, Boyer, Sébastien, Duong, Veasna, Piorkowski, Géraldine, Paupy, Christophe, Lourenco de Oliveira, Ricardo, de Lamballerie, Xavier, and Failloux, Anna Bella

Published
2019

34. A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Author

Moutailler, Sara, Yousfi, Lena, Mousson, Laurence, Devillers, Elodie, Vazeille, Marie, Vega-Rúa, Anubis, Perrin, Yvon, Jourdain, Frédéric, Chandre, Fabrice, Cannet, Arnaud, Chantilly, Sandrine, Restrepo, Johana, Guidez, Amandine, Dusfour, Isabelle, Vieira Santos de Abreu, Filipe, Pereira Dos Santos, Taissa, Jiolle, Davy, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril, Diallo, Mawlouth, Diallo, Diawo, Gaye, Alioune, Boyer, Sébastien, Duong, Veasna, Piorkowski, Géraldine, Paupy, Christophe, Lourenco de Oliveira, Ricardo, de Lamballerie, Xavier, Failloux, Anna Bella, Moutailler, Sara, Yousfi, Lena, Mousson, Laurence, Devillers, Elodie, Vazeille, Marie, Vega-Rúa, Anubis, Perrin, Yvon, Jourdain, Frédéric, Chandre, Fabrice, Cannet, Arnaud, Chantilly, Sandrine, Restrepo, Johana, Guidez, Amandine, Dusfour, Isabelle, Vieira Santos de Abreu, Filipe, Pereira Dos Santos, Taissa, Jiolle, Davy, Visser, Tessa M., Koenraadt, Constantianus J.M., Wongsokarijo, Merril, Diallo, Mawlouth, Diallo, Diawo, Gaye, Alioune, Boyer, Sébastien, Duong, Veasna, Piorkowski, Géraldine, Paupy, Christophe, Lourenco de Oliveira, Ricardo, de Lamballerie, Xavier, and Failloux, Anna Bella

Abstract

Mosquitoes are vectors of arboviruses affecting animal and human health. Arboviruses circulate primarily within an enzootic cycle and recurrent spillovers contribute to the emergence of human-adapted viruses able to initiate an urban cycle involving anthropophilic mosquitoes. The increasing volume of travel and trade offers multiple opportunities for arbovirus introduction in new regions. This scenario has been exemplified recently with the Zika pandemic. To incriminate a mosquito as vector of a pathogen, several criteria are required such as the detection of natural infections in mosquitoes. In this study, we used a high-throughput chip based on the BioMark™ Dynamic arrays system capable of detecting 64 arboviruses in a single experiment. A total of 17,958 mosquitoes collected in Zika-endemic/epidemic countries (Brazil, French Guiana, Guadeloupe, Suriname, Senegal, and Cambodia) were analyzed. Here we show that this new tool can detect endemic and epidemic viruses in different mosquito species in an epidemic context. Thus, this fast and low-cost method can be suggested as a novel epidemiological surveillance tool to identify circulating arboviruses.

Published
2019

35. A New High-Throughput Tool to Screen Mosquito-Borne Viruses in Zika Virus Endemic/Epidemic Areas

Author

Moutailler, Sara, primary, Yousfi, Lena, additional, Mousson, Laurence, additional, Devillers, Elodie, additional, Vazeille, Marie, additional, Vega-Rúa, Anubis, additional, Perrin, Yvon, additional, Jourdain, Frédéric, additional, Chandre, Fabrice, additional, Cannet, Arnaud, additional, Chantilly, Sandrine, additional, Restrepo, Johana, additional, Guidez, Amandine, additional, Dusfour, Isabelle, additional, Vieira Santos de Abreu, Filipe, additional, Pereira dos Santos, Taissa, additional, Jiolle, Davy, additional, Visser, Tessa M., additional, Koenraadt, Constantianus J. M., additional, Wongsokarijo, Merril, additional, Diallo, Mawlouth, additional, Diallo, Diawo, additional, Gaye, Alioune, additional, Boyer, Sébastien, additional, Duong, Veasna, additional, Piorkowski, Géraldine, additional, Paupy, Christophe, additional, Lourenco de Oliveira, Ricardo, additional, de Lamballerie, Xavier, additional, and Failloux, Anna-Bella, additional

Published
2019
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41. Worldwide survey reveals lower susceptibility of African Aedes aegypti mosquitoes to diverse strains of Zika virus

Author

Aubry, Fabien, primary, Martynow, Daria, additional, Baidaliuk, Artem, additional, Merkling, Sarah H., additional, Dickson, Laura B., additional, Romero-Vivas, Claudia M., additional, Vega-Rúa, Anubis, additional, Dusfour, Isabelle, additional, Jiolle, Davy, additional, Paupy, Christophe, additional, Mayanja, Martin N., additional, Lutwama, Julius J., additional, Kohl, Alain, additional, Duong, Veasna, additional, Ponlawat, Alongkot, additional, Cao-Lormeau, Van-Mai, additional, Jarman, Richard G., additional, Diagne, Cheikh T., additional, Faye, Oumar, additional, Faye, Ousmane, additional, Sall, Amadou A., additional, and Lambrechts, Louis, additional

Published
2018
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42. Diverse laboratory colonies ofAedes aegyptiharbor the same adult midgut bacterial microbiome

Author

Dickson, Laura B., primary, Ghozlane, Amine, additional, Volant, Stevenn, additional, Bouchier, Christiane, additional, Ma, Laurence, additional, Vega-Rúa, Anubis, additional, Dusfour, Isabelle, additional, Jiolle, Davy, additional, Paupy, Christophe, additional, Mayanja, Martin N., additional, Kohl, Alain, additional, Lutwama, Julius J., additional, Duong, Veasna, additional, and Lambrechts, Louis, additional

Published
2017
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43. Genetic evidence for a worldwide chaotic dispersion pattern of the arbovirus vector, Aedes albopictus

Author

Manni, Mosè, primary, Guglielmino, Carmela R., additional, Scolari, Francesca, additional, Vega-Rúa, Anubis, additional, Failloux, Anna-Bella, additional, Somboon, Pradya, additional, Lisa, Antonella, additional, Savini, Grazia, additional, Bonizzoni, Mariangela, additional, Gomulski, Ludvik M., additional, Malacrida, Anna R., additional, and Gasperi, Giuliano, additional

Published
2017
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44. Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling

Author

Dieme, Constentin, Yssouf, Amina, Vega-Rúa, Anubis, Berenger, Jean-Michel, Failloux, Anna-Bella, Raoult, Didier, Parola, Philippe, Almeras, Lionel, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], We thank Pr Ousmane Faye (Université Cheikh Anta Diop (UCAD), Dakar, Senegal) for kindly providing An. coluzzii specimens and Christophe Flaudrops (URMITE-IRD198, Marseille, France) for his advice on the analysis of MALDI-TOF MS profiles. We would like to thank Ricardo Lourenço-de-Oliveira for providing mosquitoes from Manaus in Brazil. This manuscript has been reviewed and corrected by American Journal Experts, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)

Subjects
Research, fungi, MALDI-TOF mass spectrometry, Pupa, Vectors, Infectious Diseases, Culicidae, MESH: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Species Specificity, Larva, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, parasitic diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, MESH: Biomarkers, MESH: Species Specificity, Animals, Parasitology, MESH: Animals, MESH: Pupa, MESH: Culicidae, MESH: Larva, Aquatic stages, Species identification, Biomarkers
Abstract

Background The identification of mosquito vectors is generally based on morphological criteria, but for aquatic stages, morphological characteristics may be missing, leading to incomplete or incorrect identification. The high cost of molecular biology techniques requires the development of an alternative strategy. In the last decade, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has proved to be efficient for arthropod identification at the species level. Methods To investigate the usefulness of MALDI-TOF MS for the identification of mosquitoes at aquatic stages, optimizations of sample preparation, diet, body parts and storage conditions were tested. Protein extracts of whole specimens from second larval stage to pupae were selected for the creation of a reference spectra database. The database included a total of 95 laboratory-reared specimens of 6 mosquito species, including Anopheles gambiae (S form), Anopheles coluzzi (M form), Culex pipiens pipiens, Culex pipiens molestus, Aedes aegypti and 2 colonies of Aedes albopictus. Results The present study revealed that whole specimens at aquatic stages produced reproducible and singular spectra according to the mosquito species. Moreover, MS protein profiles appeared weakly affected by the diet provided. Despite the low diversity of some MS profiles, notably for cryptic species, clustering analyses correctly classified all specimens tested at the species level followed by the clustering of early vs. late aquatic developmental stages. Discriminant mass peaks were recorded for the 6 mosquito species analyzed at larval stage 3 and the pupal stage. Querying against the reference spectra database of 149 new specimens at different aquatic stages from the 6 mosquito species revealed that 147 specimens were correctly identified at the species level and that early and late developmental stages were also distinguished. Conclusions The present work highlights that MALDI-TOF MS profiling may be useful for the rapid and reliable identification of mosquito species at aquatic stages. With this proteomic tool, it becomes now conceivable to survey mosquito breeding sites prior to the mosquitoes’ emergence and to adapt anti-vectorial measures according to the mosquito fauna detected. Electronic supplementary material The online version of this article (doi:10.1186/s13071-014-0544-0) contains supplementary material, which is available to authorized users.

Published
2014

46. Importance of mosquito “quasispecies” in selecting an epidemic arthropod-borne virus

Author

Vazeille, Marie, primary, Zouache, Karima, additional, Vega-Rúa, Anubis, additional, Thiberge, Jean-Michel, additional, Caro, Valérie, additional, Yébakima, André, additional, Mousson, Laurence, additional, Piorkowski, Géraldine, additional, Dauga, Catherine, additional, Vaney, Marie-Christine, additional, Manni, Mosè, additional, Gasperi, Giuliano, additional, de Lamballerie, Xavier, additional, and Failloux, Anna-Bella, additional

Published
2016
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48. Chikungunya Virus Transmission Potential by Local Aedes Mosquitoes in the Americas and Europe

Author

Vega-Rúa, Anubis, primary, Lourenço-de-Oliveira, Ricardo, additional, Mousson, Laurence, additional, Vazeille, Marie, additional, Fuchs, Sappho, additional, Yébakima, André, additional, Gustave, Joel, additional, Girod, Romain, additional, Dusfour, Isabelle, additional, Leparc-Goffart, Isabelle, additional, Vanlandingham, Dana L., additional, Huang, Yan-Jang S., additional, Lounibos, L. Philip, additional, Mohamed Ali, Souand, additional, Nougairede, Antoine, additional, de Lamballerie, Xavier, additional, and Failloux, Anna-Bella, additional

Published
2015
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50. Extensive variation and strain-specificity in dengue virus susceptibility among African Aedes aegypti populations.

Author

Dabo S, Henrion-Lacritick A, Lecuyer A, Jiolle D, Paupy C, Ayala D, da Veiga Leal S, Badolo A, Vega-Rúa A, Sylla M, Akorli J, Otoo S, Lutomiah J, Sang R, Mutebi JP, Saleh MC, Rose NH, McBride CS, and Lambrechts L

Abstract

African populations of the mosquito Aedes aegypti are usually considered less susceptible to infection by human-pathogenic flaviviruses than globally invasive populations found outside Africa. Although this contrast has been well documented for Zika virus (ZIKV), it is unclear to what extent it is true for dengue virus (DENV), the most prevalent flavivirus of humans. Addressing this question is complicated by substantial genetic diversity among DENV strains, most notably in the form of four genetic types (DENV1 to DENV4), that can lead to genetically specific interactions with mosquito populations. Here, we carried out a continent-wide survey of DENV susceptibility using a panel of field-derived Ae. aegypti colonies from across the African range of the species and a colony from Guadeloupe, French West Indies as non-African reference. We found considerable variation in the ability of African Ae. aegypti populations to acquire and replicate a panel of six DENV strains spanning the four DENV types. Although African Ae. aegypti populations were generally less susceptible than the reference non-African population from Guadeloupe, in several instances some African populations were equally or more susceptible than the Guadeloupe population. Moreover, the relative level of susceptibility between African mosquito populations depended on the DENV strain, indicating genetically specific interactions. We conclude that unlike ZIKV susceptibility, there is no clear-cut dichotomy in DENV susceptibility between African and non-African Ae. aegypti . DENV susceptibility of African Ae. aegypti populations is highly heterogeneous and largely governed by the specific pairing of mosquito population and DENV strain., Competing Interests: Competing interests Authors declare no competing interests.

Published
2023
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