Search

Your search keyword '"Veerkamp, Jacques H."' showing total 274 results
Start Over Author "Veerkamp, Jacques H."
274 results on '"Veerkamp, Jacques H."'

7. Fatty-acid-binding protein from the flight muscle of Locusta migratoria: Evolutionary variations in fatty acid binding

Author

Lucke, Christian, Ye Qiao, van Moerkerk, Herman T.B., Veerkamp, Jacques H., and Hamilton, James A.

Subjects
Locusts -- Physiological aspects, Locusts -- Research, Fatty acids -- Chemical properties, Protein binding -- Research, Biological sciences, Chemistry
Abstract

The structure of a second invertebrate fatty-acid-binding protein (FABP) complexed with a fatty acid is determined on the basis of intermolecular NOE connectivities between the protein and the uniformly [sup.13.C]-enriched oleate ligand. The resulting ligand conformation is characterized by certain binding features that differ significantly from the typical hairpin-turn ligand shapes of the latter forms, which is due to an alanine-to-leucine substitution in locust FABPs that produces a steric hindrance for ligand binding.

Published
2006

11. Fatty acid binding proteins from different tissues show distinct patterns of fatty acid interactions

Author

Richieri, Gary V., Ogata, Ronald T., Zimmerman, Aukje W., Veerkamp, Jacques H., and Kleinfeld, Alan M.

Subjects
Biochemistry -- Research, Fatty acids -- Research, Carrier proteins -- Research, Cells -- Analysis, Gene expression -- Analysis, Biological sciences, Chemistry
Abstract

Research has been conducted on the fatty acid binding proteins. The acrylodated intestinal fatty acid binding protein method has been used in investigating the tissue-specific cellular retinoid binding protein expression.

Published
2000

13. The use of nonhomologous scatchard analysis in the evaluation of ligand-protein interactions

Author

Zoelen, Everardus J.J. van, Kramer, Roel H., Moerkerk, Herman T.B. van, and Veerkamp, Jacques H.

Subjects
Ligand binding (Biochemistry) -- Analysis, Mathematical analysis -- Usage, Biological sciences, Chemistry, Pharmaceuticals and cosmetics industries
Abstract

Scatchard plots are widely used for the graphical presentation of receptor-ligand binding data. When a combination of labelled and unlabelled ligand molecules is used in a binding assay, equations for Scatchard plots are readily available if the labelled and unlabelled ligands have similar binding affinities. In this article, Everardus van Zoelen, Roel Kramer, Herman van Moerkerk and Jacques Veerkamp present mathematical equations to obtain the binding characteristics of an unlabelled ligand in a Scatchard plot, which has a dissociation equilibrium constant different from that of the labelled ligand used.

Published
1998

27. Absence of cardiac lipid accumulation in transgenic mice with heart-specific HSL overexpression

Author

SUZUKI, JINYA, SHEN, WEN-JUN, NELSON, BRETT D., PATEL, SHAILJA, VEERKAMP, JACQUES H., SELWOOD, SIMON P., MURPHY, GREER M., JR., REAVEN, EVE, and KRAEMER, FREDRIC B.

Subjects
Gene expression -- Analysis, Adipose tissues -- Physiological aspects, Biological sciences
Abstract

Hormone-sensitive lipase (HSL) hydrolyzes triglyceride (TG) in adipose tissue. HSL is also expressed in heart. To explore the actions of cardiac HSL, heart-specific, tetracycline (Tc)-controlled HSL-overexpressing mice were generated. Tc-responsive element-HSL transgenic (Tg) mice were generated and crossed with myosin heavy chain (MHC) [Alpha]-tTA Tg mice, which express the Tc-responsive transactivator (tTA) in the heart. The double-Tg mice (MHC-HSL) were maintained with doxycycline (Dox) to suppress Tg HSL. Upon removal of Dox, cardiac HSL activity and protein increased 12- and 8-fold, respectively, and the expression was heart specific. Although cardiac TG content increased twofold in control mice after an overnight fast, it did not increase in HSL-induced mice. Electron microscopy showed numerous lipid droplets in the myocardium of fasted control mice, whereas fasted HSL-induced mice showed virtually no droplets. Microarray analysis showed altered expression of cardiac genes for fatty acid oxidation, transcription factors, signaling molecules, cytoskeletal proteins, and histocompatibility antigens in HSL-induced mice. Thus cardiac HSL plays a role in controlling accumulation of triglyceride droplets and can affect the expression of a number of cardiac genes. hormone-sensitive lipase; gene expression; microarray analysis

Published
2001

Catalog

Books, media, physical & digital resources
See catalog results