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168 results on '"Vazeille, Emilie"'

1. Oral Curcumin No More Effective Than Placebo in Preventing Recurrence of Crohn's Disease After Surgery in a Randomized Controlled Trial

Author

Coban, Dilek, Dodel, Marie, Goutorbe, Félix, Allimant, Christophe, Reymond, Maud, Dapoigny, Michel, Rouquette, Olivier, Vazeille, Emilie, Poullenot, Florian, Riviere, Pauline, Boschetti, Gilles, Filippi, Jérôme, Del Tedesco, Emilie, Veyrard, Pauline, Pariente, Benjamin, Wils, Pauline, Zallot, Camille, Yzet, Clara, Brazier, Franck, Bommelaer, Gilles, Laharie, David, Nancey, Stéphane, Hebuterne, Xavier, Roblin, Xavier, Nachury, Maria, Peyrin-Biroulet, Laurent, Fumery, Mathurin, Richard, Damien, Pereira, Bruno, Goutte, Marion, and Buisson, Anthony

Published
2020
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8. Anti-TNF Agents Restrict Adherent-invasive Escherichia coli Replication Within Macrophages Through Modulation of Chitinase 3-like 1 in Patients with Crohn’s Disease

Author

Douadi, Clara, primary, Vazeille, Emilie, additional, Chambon, Christophe, additional, Hébraud, Michel, additional, Fargeas, Margot, additional, Dodel, Marie, additional, Coban, Dilek, additional, Pereira, Bruno, additional, Birer, Aurélien, additional, Sauvanet, Pierre, additional, Buisson, Anthony, additional, and Barnich, Nicolas, additional

Published
2022
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11. Anti-TNF agents restrict adherent-invasive E. coli replication within macrophages through modulation of chitinase 3-like 1 in patients with Crohn’s disease

Author

Douadi, Clara, Vazeille, Emilie, Chambon, C, Hébraud, Michel, Fargeas, Margot, Dodel, Marie, Coban, Dilek, Pereira, Bruno, Birer, Aurélien, Sauvanet, Pierre, Buisson, Anthony, Barnich, Nicolas, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Plateforme Exploration du Métabolisme-Composante Protéomique (PFEM-CP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Microbiologie Environnement Digestif Santé (MEDIS), Unité de Biostatistiques [CHU Clermont-Ferrand], Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), American Gastroenterological Association, and DUTILLOY, Stéphanie

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Abstract

International audience

Published
2021

12. Mannan-binding lectin deficiency results in unusual antibody production and excessive experimental colitis in response to mannose-expressing mild gut pathogens

Author

Muller, Stefan, Schaffer, Thomas, Flogerzi, Beatrice, Seibold-Schmid, Beatrice, Schnider, Jasmin, Takahashi, Kazue, Darfeuille-Michaud, Arlette, Vazeille, Emilie, Schoepfer, Alain M., and Seibold, Frank

Subjects
Mannan-binding lectin -- Health aspects, Colitis -- Risk factors, Colitis -- Genetic aspects, Enzyme-linked immunosorbent assay -- Usage, Phenotype -- Research, Phenotype -- Physiological aspects, Antibodies -- Research, Antibodies -- Physiological aspects, Viral antibodies -- Research, Viral antibodies -- Physiological aspects, Health
Published
2010

13. Genetic and microbial factors modulating the ubiquitin proteasome system in inflammatory bowel disease

Author

Cleynen, Isabelle, Vazeille, Emilie, Artieda, Marta, Verspaget, Hein W, Szczypiorska, Magdalena, Bringer, Marie-Agnès, Lakatos, Peter L, Seibold, Frank, Parnell, Kirstie, Weersma, Rinse K, Mahachie John, Jestinah M, Morgan-Walsh, Rebecca, Staelens, Dominiek, Arijs, Ingrid, De Hertogh, Gert, Müller, Stefan, Tordai, Atilla, Hommes, Daniel W, Ahmad, Tariq, Wijmenga, Cisca, Pender, Sylvia, Rutgeerts, Paul, Van Steen, Kristel, Lottaz, Daniel, Vermeire, Severine, and Darfeuille-Michaud, Arlette

Published
2014
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14. Sa531 ANTI-TNF AGENTS RESTRICT ADHERENT-INVASIVE E. COLI REPLICATION WITHIN MACROPHAGES THROUGH MODULATION OF CHITINASE 3-LIKE 1 IN PATIENTS WITH CROHN'S DISEASE

Author

Douadi, Clara, primary, Vazeille, Emilie, additional, Chambon, Christophe, additional, Hébraud, Michel, additional, Dodel, Marie, additional, Coban, Dilek, additional, Pereira, Bruno, additional, Birer, Aurélien, additional, Sauvanet, Pierre, additional, Buisson, Anthony, additional, and Barnich, Nicolas, additional

Published
2021
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16. The ubiquitin-proteasome and the mitochondria-associated apoptotic pathways are sequentially downregulated during recovery after immobilization-induced muscle atrophy

Author

Vazeille, Emilie, Codran, Audrey, Claustre, Agnes, Averous, Julien, Listrat, Anne, Bechet, Daniel, Taillandier, Daniel, Dardevet, Dominique, Attaix, Didier, and Combaret, Lydie

Subjects
Ubiquitin-proteasome system -- Physiological aspects, Atrophy, Muscular -- Physiological aspects, Biological sciences
Abstract

Immobilization produces morphological, physiological, and biochemical alterations in skeletal muscle leading to muscle atrophy and long periods of recovery. Muscle atrophy during disuse results from an imbalance between protein synthesis and proteolysis but also between apoptosis and regeneration processes. This work aimed to characterize the mechanisms underlying muscle atrophy and recovery following immobilization by studying the regulation of the mitochondria-associated apoptotic and the ubiquitin-proteasome-dependent proteolytic pathways. Animals were subjected to hindlimb immobilization for 4-8 days (I4 to I8) and allowed to recover after cast removal for 10-40 days (R10 to R40). Soleus and gastrocnemius muscles atrophied from I4 to I8 to a greater extent than extensor digitorum longus and tibialis anterior muscles. Gastrocnemius muscle atrophy was first stabilized at R10 before being progressively reduced until R40. Polyubiquitinated proteins accumulated from I4, whereas the increased ubiquitination rates and chymotrypsin-like activity of the proteasome were detectable from I6 to I8. Apoptosome and caspase-3 or -9 activities increased at I6 and I8, respectively. The ubiquitin-proteasome-dependent pathway was normalized early when muscle stops to atrophy (R10). By contrast, the mitochondria-associated apoptotic pathway was first downregulated below basal levels when muscle started to recover at R15 and completely normalized at R20. Myf 5 protein levels decreased from I4 to I8 and were normalized at R10. Altogether, our results suggest a two-stage process in which the ubiquitin-proteasome pathway is rapidly up- and downregulated when muscle atrophies and recovers, respectively, whereas apoptotic processes may be involved in the late stages of atrophy and recovery. ubiquitin-proteasome-dependent pathway; disuse; intrinsic apoptotic pathway; protein breakdown; remodeling

Published
2008

17. Quantitative proteomic analysis of macrophages from Crohn's disease patients and infected with adherent-invasive Escherichia coli : impact of anti-TNFa treatment

Author

Douadi, Clara, Vazeille, Emilie, Chambon, Christophe, Hébraud, Michel, Dodel, Marie, Pereira, Bruno, Coban, Dilek, Buisson, Anthony, Barnich, Nicolas, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie Environnement Digestif Santé (MEDIS), and Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

18. Un traitement concomitant par curcumine orale ne permet pas de diminuer le taux de récidive post-opératoire endoscopique chez les patients atteints de maladie de Crohn traités par thiopurines : résultats de l’essai contrôlé randomisé POPCUR

Author

Buisson, Anthony, Laharie, D., Nancey, S., Hebuterne, X., Roblin, X., Nachury, M., Peyrin-Biroulet, L, Fumery, Mathurin, Coban, Dilek, Goutorbe, François, Allimant, C., Vazeille, Emilie, Pereira, Brigitte, Goutte, M., Bommelaer, Gilles, CHU Clermont-Ferrand, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Service d'Hépato-gastroentérologie [CHU Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Hôpital Claude Huriez [Lille], CHU Lille, Registre EPIMAD, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Amiens-Picardie, Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Amiens-Picardie-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), and OUERTANI, jeannette

Subjects
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

19. Quantitative proteomic analysis of macrophages from Crohn's disease patients and infected with adherent-invasive Escherichia coli : impact of anti-treatment

Author

Douadi, Clara, Vazeille, Emilie, Chambon, Christophe, Hébraud, Michel, Dodel, Marie, Pereira, Bruno, Coban, Dilek, Buisson, Anthony, Barnich, Nicolas, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie Environnement Digestif Santé (MEDIS), and Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

20. Oral curcumin is not more effective than placebo to prevent endoscopic postoperative recurrence in patients with Crohn's disease treated with concomitant thiopurines: the POPCUR trial

Author

Bommelaer, Gilles, Laharie, David, Nancey, Stephane, Hebuterne, Xavier, Roblin, Xavier, Nachury, Maria, Peyrin-Biroulet, Laurent, Fumery, Mathurin, Richard, Damien, Pereira, Bruno, Goutte, Marion, Buisson, Anthony, Coban, Dilek, Goutorbe, Félix, Allimant, Christophe, Reymond, Maud, Vazeille, Emilie, CHU Clermont-Ferrand, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Autophagie infection et immunité - Autophagy Infection Immunity (APY), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Infection bactérienne, inflammation, et carcinogenèse digestive, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Université Jean Monnet [Saint-Étienne] (UJM), Hôpital Claude Huriez [Lille], CHU Lille, Registre EPIMAD, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Amiens-Picardie, Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département de Gastro-entérologie, Le CHCB, Centre Hospitalier de la Côte Basque, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Département d'hépato-gastroentérologie, CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Métis Lab EM Normandie, École de Management de Normandie (EM Normandie), Institut National de la Santé et de la Recherche Médicale (INSERM), PHRC national Association Francois Aupetit 3i Nature CHU Clermont-Ferrand, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Université Jean Monnet - Saint-Étienne (UJM), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Amiens-Picardie-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Neuro-Dol (Neuro-Dol), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité de Biostatistiques [CHU Clermont-Ferrand], Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), and Centre Hospitalier de la Côte Basque (CHCB)

Subjects
Anti-Inflammatory, Plantes médicinales, Anti-inflammatoire, Medicinal Plants, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Maladies inflammatoires intestinales, Inflammatory Bowel Diseases, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Abstract

International audience; BACKGROUND AND AIMS: Recurrence of Crohn's disease (CD) after surgery is a major concern. Curcumin has antiinflammatory properties and induces endoscopic remission in patients with ulcerative colitis. We investigated the efficacy of curcumin vs placebo in preventing post-operative recurrence of CD, based on endoscopic and clinical indices, in patients receiving concomitant thiopurine therapy.METHODS: We conducted a double-blind randomized controlled trial at 8 referral centers in France, from October 2014 through January 2018, of 62 consecutive patients with CD undergoing bowel resection. Patients received azathioprine (2.5 mg/kg) and were randomly assigned to groups given oral curcumin (3 g/day; n = 31) or an identical placebo (n = 31) for 6 months, and were then evaluated by colonoscopy. We also collected data on CD activity index, results from laboratory tests, and answers to quality of life questionnaires during this 6-month period. The primary endpoint was postoperative recurrence of CD in each group (Rutgeerts' index score >= i2) at month 6 (determined by central reading). An interim analysis (intent to treat) was scheduled after 50% of the patients were enrolled.RESULTS: At month 6, postoperative recurrence (Rutgeerts' index score >= i2) occurred in 18 patients (58%) receiving curcumin and 21 patients (68%) receiving placebo (P =.60). A significantly higher proportion of patients receiving curcumin (55%) had a severe recurrence of CD (Rutgeerts' index score >= i3) than patients receiving placebo (26%) (P =.034). We observed a clinical recurrence of CD (CD activity index score > 150) at month 6 in 45% of patients receiving placebo and 30% of patients receiving curcumin (P =.80). Quality of life scores at month 6 did not differ significantly between groups (P =.80). Severe adverse events developed in 6% of patients receiving placebo and 16% of patients receiving curcumin (P =.42).CONCLUSIONS: In a randomized controlled trial of patients who underwent surgery for CD and received thiopurine treatment, we found that curcumin was no more effective than placebo in preventing CD recurrence. There were no significant differences between groups in quality of life or severe adverse events. The study was discontinued after interim analysis due to futility.

Published
2020

21. Impact of anti-TNF-a agents on macrophages response to adherent-invasive Escherichia coli infection in patients with crohn's disease

Author

Douadi, Clara, Vazeille, Emilie, Hébraud, Michel, Dodel, Marie, Coban, Dilek, Pereira, Bruno, Buisson, Anthony, Barnich, Nicolas, OUERTANI, jeannette, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie Environnement Digestif Santé (MEDIS), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)

Subjects
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

22. Multicenter randomized double-blind cinical trial assessing the benefit of adherent-invasive E. coli eradication in adult ileal and ileo-colonic Crohn disiease: the teorem trial

Author

Carbonel, F., Vazeille, Emilie, Filippi, J, Bouhnik, Y, Moreau, J, Pariente, B, Michiels, C, Cadiot, G., Pineton de Chambrun, Guillaume, Allez, Michel, Barnich, Nicolas, Chevret, S, Buisson, Anthony, OUERTANI, jeannette, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

23. Mitophagy and mitochondria biogenesis are differentially induced in rat skeletal muscles during immobilization and/or remobilization

Author

Deval, Christiane, Calonne, Julie, Coudy-Gandilhon, Cécile, Vazeille, Emilie, Bechet, Daniel, Polge, Cécile, Taillandier, Daniel, Attaix, Didier, Combaret, Lydie, Deval, Christiane, Calonne, Julie, Coudy-Gandilhon, Cécile, Vazeille, Emilie, Bechet, Daniel, Polge, Cécile, Taillandier, Daniel, Attaix, Didier, and Combaret, Lydie

Abstract

Mitochondria alterations are a classical feature of muscle immobilization, and autophagy is required for the elimination of deficient mitochondria (mitophagy) and the maintenance of muscle mass. We focused on the regulation of mitochondrial quality control during immobilization and remobilization in rat gastrocnemius (GA) and tibialis anterior (TA) muscles, which have very different atrophy and recovery kinetics. We studied mitochondrial biogenesis, dynamic, movement along microtubules, and addressing to autophagy. Our data indicated that mitochondria quality control adapted differently to immobilization and remobilization in GA and TA muscles. Data showed i) a disruption of mitochondria dynamic that occurred earlier in the immobilized TA, ii) an overriding role of mitophagy that involved Parkin-dependent and/or independent processes during immobilization in the GA and during remobilization in the TA, and iii) increased mitochondria biogenesis during remobilization in both muscles. This strongly emphasized the need to consider several muscle groups to study the mechanisms involved in muscle atrophy and their ability to recover, in order to provide broad and/or specific clues for the development of strategies to maintain muscle mass and improve the health and quality of life of patients.

Published
2020

24. Propionate catabolism by CD-associated adherent-invasive E. coli counteracts its anti-inflammatory effect

Author

Agus, Allison, primary, Richard, Damien, additional, Faïs, Tiphanie, additional, Vazeille, Emilie, additional, Chervy, Mélissa, additional, Bonnin, Virginie, additional, Dalmasso, Guillaume, additional, Denizot, Jérémy, additional, Billard, Elisabeth, additional, Bonnet, Richard, additional, Buisson, Anthony, additional, Barnich, Nicolas, additional, and Delmas, Julien, additional

Published
2021
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26. Oral Curcumin No More Effective Than Placebo in Preventing Recurrence of Crohn's Disease After Surgery in a Randomized Controlled Trial

Author

Bommelaer, Gilles, primary, Laharie, David, additional, Nancey, Stéphane, additional, Hebuterne, Xavier, additional, Roblin, Xavier, additional, Nachury, Maria, additional, Peyrin-Biroulet, Laurent, additional, Fumery, Mathurin, additional, Richard, Damien, additional, Pereira, Bruno, additional, Goutte, Marion, additional, Buisson, Anthony, additional, Coban, Dilek, additional, Dodel, Marie, additional, Goutorbe, Félix, additional, Allimant, Christophe, additional, Reymond, Maud, additional, Dapoigny, Michel, additional, Rouquette, Olivier, additional, Vazeille, Emilie, additional, Poullenot, Florian, additional, Riviere, Pauline, additional, Boschetti, Gilles, additional, Filippi, Jérôme, additional, Del Tedesco, Emilie, additional, Veyrard, Pauline, additional, Pariente, Benjamin, additional, Wils, Pauline, additional, Zallot, Camille, additional, Yzet, Clara, additional, and Brazier, Franck, additional

Published
2020
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29. The Crohn’s disease-related AIEC strain LF82 assembles a biofilm-like matrix to protect intracellular microcolonies from phagolysosomal attack

Author

Prudent, Victoria, primary, Demarre, Gaëlle, additional, Vazeille, Emilie, additional, Wery, Maxime, additional, Ravet, Antinéa, additional, Quenech’Du, Nicole, additional, Girault, Julie Dauverd, additional, Bringer, Marie-Agnès, additional, Descrimes, Marc, additional, Barnich, Nicolas, additional, Rimsky, Sylvie, additional, Morillon, Antonin, additional, and Espéli, Olivier, additional

Published
2020
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30. Macrophages Inability to Mediate Adherent-Invasive E. coli Replication is Linked to Autophagy in Crohn’s Disease Patients

Author

Buisson, Anthony, Douadi, Clara, Ouchchane, Lemlhi, Goutte, Marion, Hugot, Jean-Pierre, Dubois, Anaëlle, Minet-Quinard, Régine, Bouvier, Damien, Bommelaer, Gilles, Vazeille, Emilie, Barnich, Nicolas, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de la Recherche Agronomique (INRA), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Broad medical program-Crohn's Colitis Foundation of America, 'Societe Nationale Francaise de Gastroenterologie' (SNFGE), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), and OUERTANI, jeannette

Subjects
MESH: macrophages / pathology, autophagy, adherent-invasive e. coli, MESH: Crohn Disease / pathology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, MESH: Crohn Disease / microbiology, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, IRGM, adherent-invasive E. coli, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, macrophages, ULK-1, MESH: Escherichia coli / pathogenicity, Crohn's disease, crohn’s disease, lcsh:Biology (General), MESH: autophagy / genetics, MESH: Escherichia coli Infections / pathology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, irgm, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ulk-1, lcsh:QH301-705.5
Abstract

The macrophages from Crohn’s Disease (CD) patients are defective to control the replication of CD-associated adherent-invasive E. coli (AIEC). We aimed to identify the host factors associated with AIEC replication focusing on polymorphisms related to autophagy. Peripheral blood monocyte-derived macrophages (MDM), obtained from 95 CD patient, 30 ulcerative colitis (UC) patients and 15 healthy subjects, were genotyped for several CD-associated polymorphisms. AIEC bacteria survival increased within MDM from CD patients compared to UC (p = 0.0019). AIEC bacteria survival increased in patients with CD-associated polymorphism IRGM (p = 0.05) and reduced in those with CD-associated polymorphisms XBP-1 (p = 0.026) and ULK-1 (p = 0.033). AIEC infection led to an increase of pro-inflammatory cytokines IL-1β (p < 0.0001) and TNF-α (p < 0.0001) in CD macrophages. ULK-1 expression increased in AIEC-infected MDM from CD patients compared to MDM from UC patients or healthy subjects (p = 0.0056) and correlated with AIEC survival (p = 0.0013). Moreover, the expression of ULK-1 phosphorylation on Serine 757 decreased following to AIEC infection (p < 0.0001). Short-term silencing of ULK-1 and IRGM genes restricted and promote, respectively, AIEC survival within MDM (p = 0.0018 and p = 0.0291). In conclusion, the macrophage defect to mediate AIEC clearance in CD patients is linked to polymorphisms related to autophagy such as IRGM and ULK-1.

Published
2019

31. Preventive effect of spontaneous physical activity on gut-adipose tissuecross-talk in CEABAC10 mice exposed by associated adherent-invasive Escherichia coli bacteria after training

Author

Maillard, Florie, Vazeille, Emilie, Sauvanet, Pierre, Sirvent, Pascal, Bonnet, Richard, Combaret, Lydie, Chausse, Pierre, Chevarin, Caroline, Otero, Yolanda, Delcros, Geoffrey, Chavanelle, Vivien, Boisseau, Nathalie, Barnich, Nicolas, Laboratoire des Adaptations Métaboliques à l’Exercice en conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA), Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Hépato-Gastro Entérologie, Centre Hospitalier Universitaire, Service de chirurgie digestive, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), service de Bacteriologie, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Laboratoire de Psychologie Sociale et Cognitive - Clermont Auvergne (LAPSCO), Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), CHU Fattouma Bourguiba [Monastir] (HFB), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire de Psychologie Sociale et Cognitive (LAPSCO), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Archive Ouverte

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], education
Abstract

communications session 3 METABOLIC DISORDERS AND MUSCLE INTEGRITY communications session 3 METABOLIC DISORDERS AND MUSCLE INTEGRITYcommunications session 3 METABOLIC DISORDERS AND MUSCLE INTEGRITY; Preventive effect of spontaneous physical activity on gut-adipose tissuecross-talk in CEABAC10 mice exposed by associated adherent-invasive Escherichia coli bacteria after training. Fifth International Congress of Translational Research in Human Nutrition (ICTRHN)

Published
2019

32. Management of mitochondria alterations depends on muscle-specific mechanical constraints during immobilization and recovery

Author

Deval, Christiane, Calonne, Julie, Coudy-Gandilhon, Cécile, Vazeille, Emilie, Béchet, Daniel, Polge, Cécile, Taillandier, Daniel, Attaix, Didier, Combaret, Lydie, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Deparment Endocrinology Metabolism & Cardiovascular System, Faculty of Science and Medicine, Université de Fribourg, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Hépato-Gastro Entérologie, CHU Fattouma Bourguiba [Monastir] (HFB), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Université Clermont Auvergne (UCA), Centre Hospitalier Universitaire, ProdInra, Archive Ouverte, University of Fribourg, and Université de Fribourg = University of Fribourg (UNIFR)

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, education, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, health care economics and organizations
Abstract

Management of mitochondria alterations depends on muscle-specific mechanical constraints during immobilization and recovery. Fifth International Congress of Translational Research in Human Nutrition (ICTRHN)

Published
2019

33. High intensity interval training promotes total and visceral fat mass loss in obese Zucker rats without modulating gut microbiota

Author

Maillard, Florie, Vazeille, Emilie, Sauvanet, Pierre, Sirvent, Pascal, Combaret, Lydie, Soudrille, Antoine, Chavanelle, Vivien, Bonnet, Richard, Otero, Yolanda, Delcros, Geoffrey, Barnich, Nicolas, Boisseau, Nathalie, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Service de Chirurgie Digestive et Hépatobiliaire [CHU Clermont-Ferrand], Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Service de Bactériologie [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], Region Auvergne-Rhone-Alpes (PREVAMIC project), I-SITE project (CAP 2025), Laboratoire des Adaptations Métaboliques à l’Exercice en conditions Physiologiques et Pathologiques (AME2P), Institut National de la Santé et de la Recherche Médicale (INSERM), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Service d'Hépato-Gastro Entérologie, Centre Hospitalier Universitaire, Service de chirurgie digestive, Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), service de Bacteriologie, and ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016)

Subjects
Male, Membrane Glycoproteins, Science, Intra-Abdominal Fat, Receptors, Adrenergic, alpha, digestive system, Gastrointestinal Microbiome, Rats, Rats, Zucker, Glucose, Gene Expression Regulation, Occludin, Physical Conditioning, Animal, Receptors, Adrenergic, beta, Body Composition, Zonula Occludens-1 Protein, Animals, Medicine, Carrier Proteins, Energy Metabolism, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Acute-Phase Proteins
Abstract

AimsIncreased visceral adipose tissue and dysbiosis in the overweight and obese promote chronic inflammation. The aim of this study was to compare the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the gut-adipose tissue cross-talk in obese Zucker rats.MethodsObese male Zucker rats (n = 36) were divided in three groups: MICT (12m.min-1 for 51min), HIIT (6 sets at 18 m.min-1 for 4min followed by 3min at 10m.min-1) and controls (CONT; no exercise). The animals ran on a treadmill 5 days/week for 10 weeks. Body composition, glycaemic control, lipid profile, inflammation, lipolysis signalling in subcutaneous and visceral adipose tissue, intestinal permeability (tight junctions and plasma lipopolysaccharide binding protein; LBP), and gut microbiota composition were assessed in the three groups.ResultsAfter 10 weeks of exercise, total and epididymal fat mass decreased only in the HIIT group. The α/β adrenergic receptor RNA ratio in subcutaneous adipose tissue increased only in the HIIT group. The expression level of phosphorylated hormone-sensitive lipase was not modified by training. Both HIIT and MICT decreased inflammation (plasma myeloperoxidase and keratinocyte-derived chemokine secretion in adipose tissue) and improved glucose metabolism. Zonula occludens-1 and occludin were upregulated in the HIIT group. Plasma LBP was similarly reduced in both training groups. HIIT and MICT did not affect gut microbiota composition.ConclusionIn obese Zucker rats, HIIT and MICT improved inflammation and glucose metabolism. In contrast, only HIIT decreased total and visceral fat mass. These adaptations were not associated with modifications in gut microbiota composition.

Published
2019

35. Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility

Author

Maillard, Florie, Vazeille, Emilie, Sauvanet, Pierre, Sirvent, Pascal, Bonnet, Richard, Combaret, Lydie, Chausse, Pierre, Chevarin, Caroline, Otero, Yolanda, Delcros, Geoffrey, Chavanelle, Vivien, Boisseau, Nathalie, and Barnich, Nicolas

Subjects
Santé publique et épidémiologie, Crohn’s disease, mesenteric adipose tissue, physical activity, Autre (Sciences du Vivant)
Abstract

Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics Crohn's disease susceptibility. Thirty-five CEABAC10 male mice performed spontaneous PA (wheel group; n = 24) or not (controls; n = 11) for 12 weeks. At week 12, mice were orally challenged with the AIEC LF82 strain for 6 days. Body composition, glycaemic control, intestinal permeability, gut microbiota composition, and fecal short-chain fatty acids were assessed in both groups. Animals were fed a high fat/high sugar diet throughout the study. After exposure to AIEC, mesenteric adipose tissue weight was lower in the wheel group. Tight junction proteins expression increased with spontaneous PA, whereas systemic lipopolysaccharides were negatively correlated with the covered distance. Bifidobacterium and Lactobacillus decreased in controls, whereas Oscillospira and Ruminococcus increased in the wheel group. Fecal propionate and butyrate were also higher in the wheel group. In conclusion, spontaneous physical activity promotes healthy gut microbiota composition changes and increases short-chain fatty acids in CEABAC10 mice fed a Western diet and exposed to AIEC to mimic Crohn's disease.

Published
2019

36. Parkin-Dependent Pathway is differentially regulated in immobilized ratGastronemius and Tibialis anterior muscles

Author

Calonne, Julie, Deval, Christiane, Coudy-Gandilhon, Cécile, Vazeille, Emilie, Dardevet, Dominique, Béchet, Daniel, Polge, Cécile, Taillandier, Daniel, Attaix, Didier, Combaret, Lydie, Department of Medicine, PUCRS School of Medicine, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut National de la Santé et de la Recherche Médicale (INSERM), Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Club Francophone de l'Autophagie (CFATG). FRA., Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), UMR 1071, Université Clermont Auvergne (UCA), ProdInra, Archive Ouverte, and Société Française de Biochimie et Biologie Moléculaire (SFBBM). FRA.

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, mitophagy, mitochondria remodeling, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, parkin-dependent pathway, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, skeletal muscle disuse
Abstract

Session 4: Proteolysis in health and diseasesSession 4: Proteolysis in health and diseases; Periods of immobilization or acute inactivity are associated with weakness and/or frailty, and further contribute to muscle atrophy and elevated healthcare costs. Muscle wasting is often associated with mitochondria (mt) alterations and autophagy is required for muscle mass maintenance through the elimination of defective mitochondria. Tibialis anterior (TA) and gastrocnemius (GA) rat muscles exhibit different mitochondria-associated apoptotic responses to immobilization and recovery (1). We therefore investigated the regulation of mitochondria quality control in the TA and the GA following 8 days of immobilization (I8) and 6 days of remobilization (R6) in rats. The mtDNA / genomic DNA ratio decreased in both muscles at I8 and R6, suggesting a decrease in mitochondria abundance. The levels for the fission protein Fis1 were elevated in the TA at I8 and in both muscles at R6, while levels for the fusion proteins Opa1 and Mfn2 were elevated at R6 only in the TA. Thus, fusion and fission processes were imbalanced during immobilization and remobilization, underwent muscle-specific alterations, and followed a different temporal regulation in the TA and the GA. The Pink-Parkin dependent selective pathway for addressing altered mitochondria to autophagy also showed a muscle-specific regulation. Indeed, only the immobilized and/or remobilized GA exhibited elevated mRNA levels for Pink and Parkin and protein levels for VDAC1. However, P62 protein levels increased during immobilization in both muscles at I8 and remained elevated only in the GA during remobilization. However, although this suggest a selective addressing altered mitochondria to autophagy in both muscles, Rab32 mRNA levels and LC3 lipidation increased only in the TA during immobilization and remobilization. Altogether, we suggest that the addressing altered mitochondria for elimination following immobilization involved different muscle-specific mechanisms that should be further investigated. They may include either a different temporal regulation of the Pink-Parkin dependent pathway in the GA and the TA and/or a muscle specific regulation of the LC3 dependent pathway in the TA and the GA. The latter may imply the formation of mitochondria-derived vesicles in the GA that would be directly driven to late endosomes independently of LC3.Reference (1) Slimani L, Micol D, Amat J, Delcros G, Meunier B, Taillandier D, Polge C, Béchet D, Dardevet D, Picard B, Attaix D, Listrat A, Combaret L. The worsening of tibialis anterior muscle atrophy during recovery post-immobilization correlates with enhanced connective tissue area, proteolysis, and apoptosis. Am J Physiol Endocrinol Metab. 2012, 303, E1335-47.

Published
2018

38. Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model

Author

Groussard, Carole, primary, Maillard, Florie, additional, Vazeille, Emilie, additional, Barnich, Nicolas, additional, Sirvent, Pascal, additional, Otero, Yolanda F., additional, Combaret, Lydie, additional, Madeuf, Elise, additional, Sourdrille, Antoine, additional, Delcros, Geoffroy, additional, Etienne, Monique, additional, Teixeira, Allison, additional, Sauvanet, Pierre, additional, Pialoux, Vincent, additional, and Boisseau, Nathalie, additional

Published
2019
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39. Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn’s Disease Susceptibility

Author

Maillard, Florie, primary, Vazeille, Emilie, additional, Sauvanet, Pierre, additional, Sirvent, Pascal, additional, Bonnet, Richard, additional, Combaret, Lydie, additional, Chausse, Pierre, additional, Chevarin, Caroline, additional, Otero, Yolanda Fernandez, additional, Delcros, Geoffrey, additional, Chavanelle, Vivien, additional, Boisseau, Nathalie, additional, and Barnich, Nicolas, additional

Published
2019
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40. The delayed recovery of the remobilized rat tibialis anterior muscle reflects a defect in proliferative and terminal differentiation that impairs early regenerative processes

Author

Slimani, Lamia, Vazeille, Emilie, Deval, Christiane, Meunier, Bruno, Polge, Cécile, Dardevet, Dominique, Bechet, Daniel, Taillandier, Daniel, Micol, Didier, Listrat, Anne, Attaix, Didier, Combaret, Lydie, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
β-Catenin, [SDV]Life Sciences [q-bio], matrice extracellulaire, beta-catenin, Original Articles, Extracellular matrix, Outside-In signaling, beta catenine, atrophie musculaire, extracellular matrix, outside-in signaling, recovery, regeneration, atrophy, muscular atrophy, Recovery, beta catenin, Regeneration, rat, shelterwood felling, regénération, Skeletal muscle atrophy
Abstract

Background The immobilization-induced tibialis anterior (TA) muscle atrophy worsens after cast removal and is associated with altered extracellular matrix (ECM) composition. The secreted protein acidic and rich in cysteine (Sparc) is an ECM component involved in Akt activation and in β-catenin stabilization, which controls protein turnover and induces muscle regulatory factors (MRFs), respectively. We hypothesized that ECM alterations may influence these intracellular signalling pathways controlling TA muscle mass. Methods Six-month-old Wistar rats were subjected to hindlimb cast immobilization for 8 days (I8) or not (I0) and allowed to recover for 1 to 10 days (R1–10). Results The TA atrophy during remobilization correlated with reduced fibre cross-sectional area and thickening of endomysium. mRNA levels for Sparc increased during remobilization until R10 and for integrin-α7 and -β1 at I8 and R1. Integrin-linked kinase protein levels increased during immobilization and remobilization until R10. This was inversely correlated with changes in Akt phosphorylation. β-Catenin protein levels increased in the remobilized TA at R1 and R10. mRNA levels of the proliferative MRFs (Myf5 and MyoD) increased at I8 and R1, respectively, without changes in Myf5 protein levels. In contrast, myogenin mRNA levels (a terminal differentiation MRF) decreased at R1, but only increased at R10 in remobilized muscles, as for protein levels. Conclusions Altogether, this suggests that the TA inefficiently attempted to preserve regeneration during immobilization by increasing transcription of proliferative MRFs, and that the TA could engage recovery during remobilization only when the terminal differentiation step of regeneration is enhanced.

Published
2015

41. Faster and less invasive tools to identify patients with ileal colonization by adherent‐invasive E. coliin Crohn's disease

Author

Buisson, Anthony, Vazeille, Emilie, Fumery, Mathurin, Pariente, Benjamin, Nancey, Stéphane, Seksik, Philippe, Peyrin‐Biroulet, Laurent, Allez, Matthieu, Ballet, Nathalie, Filippi, Jérôme, Yzet, Clara, Nachury, Maria, Boschetti, Gilles, Billard, Elisabeth, Dubois, Anaëlle, Rodriguez, Stéphanie, Chevarin, Caroline, Goutte, Marion, Bommelaer, Gilles, Pereira, Bruno, Hebuterne, Xavier, and Barnich, Nicolas

Abstract

The identification of Crohn's disease (CD)‐associated adherent and invasive Escherichia coli(AIEC) is time‐consuming and requires ileal biopsies. We aimed to identify a faster and less invasive methods to detect ileal colonization by AIEC in CD patients. CD patients requiring ileo‐colonoscopy were consecutively enrolled in this prospective multicenter study. Samples from saliva, serum, stools, and ileal biopsies of CD patients were collected. Among 102 CD patients, the prevalence of AIEC on ileal biopsies was 24.5%. The abundance and global invasive ability of ileal‐associated total E. coliwere respectively ten‐fold (p= 0.0065) and two‐fold (p= 0.0007) higher in AIEC‐positive (vs. AIEC‐negative), while abundance of total E. coliin the feces was not correlated with AIEC status in the ileum. The best threshold of ileal total E. coliwas 60 cfu/biopsy to detect AIEC‐positive patients, with high negative predictive value (NPV) (94.1%[80.3–99.3]), while the global invasive ability (>9000 internalized bacteria) was able to detect the presence of AIEC with high positive predictive value (80.0% [55.2–100.0]). Overall, 78.1% of the AIEC + patients were colonized by two or less different AIEC strains. The level of serum anti‐total E. coliantibodies (AEcAb) was higher in AIEC‐positive patients (p= 0.038) with a very high negative predictive value (96.6% [89.9–100.0]) (p= 0.038) for a cut‐off value > 1.9 × 10−3. More than two thirds of AIEC‐positive CD patients were colonized by two or less AIEC strains. While stools samples are not accurate to screen AIEC status, the AEcAb level appears to be an attractive, rapid and easier biomarker to identify patients with Crohn's disease harboring AIEC.

Published
2021
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42. Macrophages from Crohn's disease patients showed a defect to control adherent-invasive Escherichia coli replication influenced by genetic host factors

Author

Buisson, A., Vazeille, Emilie, Ouchchane, L., Goutte, Marion, Hugot, J. -P., Dubois, André, Quinard, R. Minet, Bouvier, D., Bommelaer, G., Barnich, Nicolas, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Département Gastroentérologie, CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Unité de biostatistique, CRLC Val d'Aurelle, Université Paris Diderot - Paris 7 (UPD7), Hôpital Ambroise Paré [AP-HP], CHU Gabriel Montpied [Clermont-Ferrand], and CHU Clermont-Ferrand

Subjects
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

43. Postoperative Complications after Ileocecal Resection in Crohn’s Disease: A Prospective Study From the REMIND Group

Author

Fumery , Mathurin, Seksik , Philippe, Auzolle , Claire, Munoz-Bongrand , Nicolas, Gornet , Marc, Boschetti , Gilles, Cotte , Eddy, Buisson , Anthony, Dubois , Anne, Pariente , Benjamin, Zerbib , Philippe, Chafai , Najim, Stefanescu , Carmen, PANIS , Yves, Marteau , Philippe, Pautrat , Karine, Sabbagh , Charles, Filippi , Jerôme, Chevrier , Marc, Houzé , Pascal, Jouven , Xavier, Tréton , Xavier, Allez , Matthieu, Cattan , Pierre, Chirica , Mircea, Corte , Hélène, Baudry , Clotilde, Lourenco , Nelson, Tran-Minh , My-Linh, Maillet , Maria, Bonnet , Joelle, Chedouba , Leila, Lebourhis , Lionel, Brot , Loic, Chardiny , Victor, Grand , Céline, Nisard , Andrée, Beaugerie , Laurent, Cosnes , Jacques, Rajca , Sylvie, Landman , Cecilia, SOKOL , Harry, Lefevre , Jérémie, Svreck , Magali, Hoyau-Idrissi , Nadia, Maggiori , Léon, Bouhnik , Yoram, Corcos , Olivier, Dray , Xavier, Chaput , Ulrika, Goutte , Marion, Bommelaer , Gilles, Vazeille , Emilie, BARNICH , Nicolas, Nachury , Maria, Desreumaux , Pierre, Sommeville , Coralie, Dupas , Jean-Louis, Brazier , Franck, Chatelain , Denis, Attencourt , Christophe, Leconte, Martine, Thiebault , Henri, Loreau , Julien, Nancey , Stephane, Flourié , Bernard, François , Yves, Charlois , Anne-Laure, Falgon , Peggy, Moussata , Driffa, Chauvenet , Marion, Boyer , Sarah, Hebuterne , Xavier, Hofmann , Paul, Margalef , Carole, Djenidi , Faycal, Detre , Patricia, Centre hospitalier universitaire d'Amiens ( CHU Amiens-Picardie ), CHU Amiens-Picardie, Service de Gastroentérologie et nutrition [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Saint-Antoine [APHP], INSERM, U1160, Département de Gastroentérologie, Université Paris Diderot (Paris 7), Department of Gastroenterological Surgery, Hop St Louis, Centre International de Recherche en Infectiologie ( CIRI ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Service de chirurgie oncologique, Hospices Civils de Lyon ( HCL ), CHU Clermont-Ferrand, Modèles et méthodes de l'évaluation thérapeutique des maladies chroniques, Université Paris Diderot - Paris 7 ( UPD7 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ), Digestive Surgery and Transplantation, Service de chirurgie générale et digestive [CHU Saint-Antoine], Pole des maladies de l'appareil digestif, gastroentérologie et assistance nutritive, AP-HP Hôpital Beaujon, Aix-Marseille Université - Faculté de médecine ( AMU MED ), Aix Marseille Université ( AMU ), Service de gastro-entérologie, Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Lariboisière, Hôpital Lariboisière, Department Digestion and Oncology Surgery, Université de Picardie Jules Verne ( UPJV ), Hépato-Gastroentérologie, CHU Nice, Biochimie, Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Trousseau [APHP], Hôpital Saint-Louis, Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Diderot - Paris 7 ( UPD7 ), Epidémiologie cardiovasculaire et mort subite, Université Paris-Sud - Paris 11 ( UP11 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre de recherche biomédicale Bichat-Beaujon ( CRB3 ), Cell Therapy Unit, Assistance publique - Hôpitaux de Paris (AP-HP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris]-Université Paris Diderot - Paris 7 ( UPD7 ), Gastroenterology, Saint-Louis Hospital, School of Electrical and Computer Engineering, Georgia Institute of Technology [Atlanta], Université Pierre et Marie Curie - Paris 6 ( UPMC ), Microorganismes, Molécules Bioactives et Physiopathologie Intestinale ( LBM-E4 ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), MICrobiologie de l'ALImentation au Service de la Santé humaine ( MICALIS ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Association Pro Pictura Antiqua ( APPA-CEPMR ), Ville de Soissons, Hôpital Beaujon, Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Diderot - Paris 7 ( UPD7 ) -Hôpital Beaujon, Serv Gastroenterol & Assistance Nutr, ASTRE [Cergy-Pontoise], Equipes Traitement de l'Information et Systèmes ( ETIS ), Ecole Nationale Supérieure de l'Electronique et de ses Applications ( ENSEA ) -Université de Cergy Pontoise ( UCP ), Université Paris-Seine-Université Paris-Seine-Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Supérieure de l'Electronique et de ses Applications ( ENSEA ) -Université de Cergy Pontoise ( UCP ), Université Paris-Seine-Université Paris-Seine-Centre National de la Recherche Scientifique ( CNRS ), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne ( M2iSH ), Université Clermont Auvergne ( UCA ), Institut National de la Santé et de la Recherche Médicale, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte ( M2iSH ), Institut National de la Recherche Agronomique ( INRA ) -Université d'Auvergne - Clermont-Ferrand I ( UdA ), Département Microbiologie et Chaîne Alimentaire ( MICA ), Institut National de la Recherche Agronomique ( INRA ), Département de Gastroentérologie, Hôpital Européen [Fondation Ambroise Paré - Marseille], Hepato-Gastroenterologie, Centre Hospitalier Régional Universitaire de Tours, CHRU Tours, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), and Centre Hospitalier Universitaire de Nice ( CHU de Nice )

Subjects
[ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

44. Macrophages Versus Escherichia coli

Author

Buisson, Anthony, Bringer, Marie-Agnès, Barnich, Nicolas, Vazeille, Emilie, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne ( M2iSH ), Université Clermont Auvergne ( UCA ), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte ( M2iSH ), Institut National de la Recherche Agronomique ( INRA ) -Université d'Auvergne - Clermont-Ferrand I ( UdA ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), CHU Estaing [Clermont-Ferrand], Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)

Subjects
inflammatory-bowel-disease, nitric-oxide synthase, monocyte-derived macrophages, Macrophages, E. coli, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, invasive e. coli, necrosis-factor-alpha, susceptibility loci, Crohn's disease, anti-saccharomyces-cerevisiae, nf-kappa-b, genome-wide association, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, intestinal epithelial-cells
Abstract

International audience; The pathophysiology of Crohn's disease (CD), a chronic inflammatory bowel disease, remains imperfectly elucidated. Consequently, the therapeutic armamentarium remains limited and has not changed the natural history of CD hitherto. Accordingly, physicians need to identify new therapeutic targets to be able to alter the intestinal damage. The most recent hypothesis considered CD as resulting from an abnormal interaction between microbiota and host immune system influenced by genetics and environmental factors. Several experimental and genetic evidence point out intestinal macrophages in CD etiology. An increase of macrophages number and the presence of granulomas are especially observed in the intestinal mucosa of patients with CD. These macrophages could be defective and particularly in responses to infectious agents like CD-associated Escherichia coli. This review focuses on, what is currently known regarding the role of macrophages, macrophages/E. coli interaction, and the impact of CD therapies on macrophages in CD. We also speculate that macrophages modulation could lead to important translational implications in CD with the end goal of promoting gut health.

Published
2016

45. Su1798 - Comparison Between Faecal Chitinase 3-Like 1, Matrix Metalloprotease 9 and Calprotectin to Assess Mucosal Healing in Patients with Crohn's Disease: A Multicenter Prospective Study

Author

Buisson, Anthony, primary, Fumery, Mathurin, additional, Hebuterne, Xavier, additional, Pariente, Benjamin, additional, Nancey, Stephane, additional, Minet-Quinard, Régine, additional, Peyrin-Biroulet, Laurent, additional, Seksik, Philippe, additional, Allez, Matthieu, additional, Lambert, Céline, additional, Dupas, Jean-Louis, additional, Filippi, Jerome, additional, Nachury, Maria, additional, Boschetti, Gilles, additional, Goutte, Marion, additional, Pereira, Bruno, additional, Bouvier, Damien, additional, Bommelaer, Gilles, additional, Barnich, Nicolas, additional, and Vazeille, Emilie, additional

Published
2018
Full Text
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46. Tu1861 - Autophagy-Related Host Factors are Implicated in the Incapability of Macrophages from Chohn's Disease Patients to Mediate Adherent-Invasive E.Coli Clearance

Author

Douadi, Clara, primary, Buisson, Anthony, additional, Ouchchane, Lemlih, additional, Goutte, Marion, additional, Hugot, Jean-Pierre, additional, Dubois, Anaelle, additional, Minet-Quinard, Régine, additional, Bouvier, Damien, additional, Bommelaer, Gilles, additional, Vazeille, Emilie, additional, and Barnich, Nicolas, additional

Published
2018
Full Text
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47. Faecal metalloprotease 9 is a reliable biomarker compared with faecal calprotectin in detecting endoscopic activity in patients with inflammatory bowel diseases

Author

Buisson, Alain, Vazeille, Emilie, Quinard, R. M., Goutte, Marion, Bouvier, D., Goutorbe, F., Pereira, B., BARNICH, Nicolas, Bommelaer, G., Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA), Laboratoire de Biochimie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Département Gastroentérologie, CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Published
2016

48. Macrophages from Crohn's Disease Patients Showed a Defect to Control Adherent- Invasive E. Coli Replication Influenced by Genetic Host Factors

Author

Buisson, Anthony, primary, Vazeille, Emilie, additional, Ouchchane, Lemlih, additional, Goutte, Marion, additional, Hugot, Jean-Pierre, additional, Dubois, Anaelle, additional, Minet-Quinard, Régine, additional, Bouvier, Damien, additional, Bommelaer, Gilles, additional, and Barnich, Nicolas, additional

Published
2017
Full Text
View/download PDF

49. Type 1 pili and long polar fimbriae are essential for induction of host microbiota mutualism by adherent-invasive Escherichia coli, even in the presence of competing intestinal microbes

Author

Vazeille, Emilie, Balmer, M. L., Chassaing, B., Schaffer, T. T., Slack, E., Pfister, S., Geuking, M. B., Lottaz, Daniel, Macpherson, Andrew J., Hapfelmeier, S., Mccoy, Karen D, Darfeuille-Michaud, Arlette, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA), Universität Bern- University of Bern [Bern], Partenaires INRAE, and Institute of Microbiology

Subjects
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Published
2014

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