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Toxoplasmosis is a disease that caused by Toxoplasma gondii parasite. Toxoplasma gondii is a group of protozoa, and it belongs to intracellular obligate parasites. This parasite mostly found in animal, and it can be dangerous, on many cases, it can cause death with no symptoms. The purpose of this study is to find out the percentage of IgG and IgM antibodies examination of Toxoplasma gondii on blood donor in PMI Kota Yogyakarta. This study belongs to descriptive observational type, with cross-sectional design to find out the results of antibody detection on Toxoplasma gondii. The detection was carried out by Rapid Diagnostic Methods that can detect the presence of IgG and IgM antibodies. This study was also using questionnaires to know the relationship between the daily behaviours with toxoplasmosis case. The results show that 14 samples were reactive on IgG antibody parameter (76%) and 11 samples were non-reactive (24%). From the questionnaires known that the daily behaviour’s such as having a cat, eating raw/undercooked meat products, eating unwashed fruits and vegetables related to the case of toxoplasmosis.