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2. High-resolution two-photon, three-photon, Harmonic generations and CARS flexible endo-microscope

Author

Vasyl Mytskaniuk, Septier, D., Gaëlle Brevalle, Victor Tsvirkun, Fabert Marc, Géraud Bouwmans, Frédéric Louradour, Kudlinski, A., Hervé RIGNEAULT, Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM), Université de Lille-Centre National de la Recherche Scientifique (CNRS), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), MOSAIC (MOSAIC), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), and Lightcore Technologies (LCT)

Subjects
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2022

3. High-resolution multimodal flexible coherent Raman endoscope (Conference Presentation)

Author

Viktor Tsvirkun, Hervé Rigneault, Frédéric Louradour, Alexandre Kudlinski, Marc Fabert, and Vasyl Mytskaniuk

Subjects
Materials science, business.industry, Physics::Optics, Second-harmonic generation, law.invention, Lens (optics), Core (optical fiber), symbols.namesake, Optics, law, Picosecond, Femtosecond, symbols, business, Raman spectroscopy, Image resolution, Raman scattering
Abstract

We present a flexible, compact, and multimodal nonlinear endoscope (2.2 mm outer diameter) based on a resonantly scanned negative curvature hollow-core double-clad fiber. The fiber design allows distortion-less, background-free delivery of femtosecond and picosecond excitation pulses and the back-collection of nonlinear signals through the same fiber. Sub-micron spatial resolution together with large field of view is made possible by the combination of a miniature objective lens together with a silica microsphere lens inserted into the fiber core. We demonstrate coherent anti-Stokes Raman scattering, 2-photon fluorescence and second harmonic generation imaging at a rate of 10 frames/s.

Published
2020

4. High-resolution multimodal flexible coherent Raman endoscope

Author

Hervé RIGNEAULT, Viktor Tsvirkun, Vasyl Mytskaniuk, Fabert Marc, Frédéric Louradour, Kudlinski, A., Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), MOSAIC (MOSAIC), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Photonique Fibre et Sources Cohérentes (XLIM-PHOT), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), and Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)

Subjects
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

5. Double clad tubular anti-resonant hollow core fiber for nonlinear microendoscopy

Author

Vasyl Mytskaniuk, D. Septier, Géraud Bouwmans, Alexandre Kudlinski, Viktor Tsvirkun, Olivier Vanvincq, Andy Cassez, Karen Baudelle, Rémi Habert, Hervé Rigneault, A. Pastre, Marc Douay, Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche sur les Composants logiciels et matériels pour l'Information et la Communication Avancé - USR 3380 (IRCICA), Institut FRESNEL (FRESNEL), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), MOSAIC (MOSAIC), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), and ANR-19-CE19-0019,MEAP,Endomicroscopie multiphotonique des plaques athérosclérotiques(2019)

Subjects
Hollow core, Total internal reflection, Fabrication, Materials science, business.industry, Physics::Optics, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Atomic and Molecular Physics, and Optics, Light scattering, Numerical aperture, 010309 optics, Nonlinear system, symbols.namesake, Optics, 0103 physical sciences, symbols, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Fiber, 0210 nano-technology, business, Raman scattering
Abstract

International audience; We report the fabrication and characterization of the first double clad tubular anti-resonant hollow core fiber. It allows to deliver ultrashort pulses without temporal nor spectral distortions in the 700-1000 nm wavelength range and to efficiently collect scattered light in a high numerical aperture double clad. The output fiber mode is shaped with a silica microsphere generating a photonic nanojet, making it well suitable for nonlinear microendoscopy application. Additionally, we provide an open access software allowing to find optimal drawing parameters for the fabrication of tubular hollow core fibers.

Published
2020

6. High-resolution multimodal flexible miniaturized endoscope for non-linear imaging (Conference Presentation)

Author

Alberto Lombardini, Frédéric Louradour, Alexandre Kudlinski, Vasyl Mytskaniuk, Esben Ravn Andresen, Nicolas Joly, Xueqin Chen, Marc Fabert, Hervé Rigneault, Jérôme Wenger, Siddharth Sivankutty, Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), MOSAIC (MOSAIC), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Centre de Photonique Biomédicale (CPBM), Centre Laser Université Paris Sud, Photonique Fibre et Sources Cohérentes (XLIM-PHOT), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Centre Technologique des Agroressources, Université d'Artois (UA)-IUT de Bethunes, Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Fluorescence-lifetime imaging microscopy, Materials science, Scattering, business.industry, Field of view, 02 engineering and technology, 021001 nanoscience & nanotechnology, Frame rate, 01 natural sciences, 010309 optics, symbols.namesake, Optics, 0103 physical sciences, Femtosecond, symbols, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Fiber, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, 0210 nano-technology, business, Excitation, Raman scattering, ComputingMilieux_MISCELLANEOUS
Abstract

Non-linear optical microscopy proves to be an indispensable tool in natural sciences and becomes more and more attractive for clinical applications. Coherent Raman scattering, for instance, has the potential to become an in-vivo fast label-free histology tool as its chemical selectivity provides quantitative information on lipids and proteins locations and concentrations in tissues. Along with these techniques, second-harmonic generation of collagen and 2-photon excitation fluorescence broaden even more the non-linear imaging ability as collagen fibers represent an important role in human body construction. Whilst 2-photon excitation fluorescence allows to study auto-fluorescence (ex. NADH and NADHP molecules), and to excite a vast range of chromophores. However, absorption and scattering limit significantly the nonlinear imaging depth into tissues. As a solution, we offer a flexible, compact, and multimodal nonlinear endoscope (2.2 mm outer diameter, 35 mm rigid length) based on a resonantly piezo scanned hollow-core negative curvature double-clad fiber. The fiber design allows distortion-less, background-free delivery of femtosecond excitation pulses and the back-collection of nonlinear signals through the same fiber. The double-cladding of this fiber attends 10^5μm of silica collection surface which allows for a 4-fold collection improvement compared to previously used Kagome hollow core fibers. Having a good control on the resonantly scanning fiber the endoscope can perform nonlinear imaging up to 8 frames per second over a field of view of 400μm. We demonstrate 2photon, SHG and CARS imaging in ex vivo gastric human tissue samples and in-vivo 2-photon fluorescence imaging of GFP-labeled neurons in mouse brain.

Published
2019

7. High-resolution multimodal flexible coherent Raman endoscope

Author

Vasyl Mytskaniuk, Siddharth Sivankutty, Esben Ravn Andresen, Jérôme Wenger, Nicolas Joly, Fabert Marc, Frédéric Louradour, Alexandre Kudlinski, Hervé RIGNEAULT, Institut FRESNEL (FRESNEL), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), MOSAIC (MOSAIC), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Max Planck Institute for the Science of Light, Max-Planck-Gesellschaft, XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Photonique Fibre et Sources Cohérentes (XLIM-PHOT), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 (PhLAM), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), and Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

8. Parasitic signals in hollow-core fibers used for SRS endoscopy

Author

Ingo Rimke, Alberto Lombardini, Hervé Rigneault, Alexandre Kudlinski, Esben Ravn Andresen, Olivier Vanvincq, and Vasyl Mytskaniuk

Subjects
Materials science, Optical fiber, Microscope, business.industry, Cross-phase modulation, Physics::Optics, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, law.invention, 010309 optics, symbols.namesake, Optics, Physics::Plasma Physics, law, 0103 physical sciences, Microscopy, symbols, Fiber, Photonics, 0210 nano-technology, business, Raman spectroscopy, Raman scattering
Abstract

Stimulated Raman scattering (SRS) microscopy [1] appears nowadays as a viable platform with strong potential in biology and medicine. The implementation of SRS is generally accompanied by parasitic signals stemming from other quasi-instantaneous nonlinear optical processes such as two-photon absorption, cross phase modulation (XPM), and thermal effects. Implementations which involve propagation of the excitation pulses in optical fiber, i.e. fiber delivery for SRS microscopes and SRS endoscopes are much more prone to generate parasitic signals. Hollow-core fibers, like photonic band-gap fibers and Kagome-lattice hollow-core fibers (KL-HCF), are generally thought to minimize parasitic signals because light propagates in air rather than silica. However, in [2] we reported CARS and SRS using hollow core fiber; although Raman spectral features from the sample could be retrieved, these signals sat on a plateau whose origin was uncertain.

Published
2017

9. Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Author

Vasyl Mytskaniuk, Hervé Rigneault, Fabrice Bardin, Hassan Boukhaddaoui, Nicolas Tricaud, Institut des Neurosciences de Montpellier - Déficits sensoriels et moteurs (INM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut d’Electronique et des Systèmes (IES), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matériaux, MicroCapteurs et Acoustique (M2A), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Mathématiques, Informatique, Physique, et Applications / Université de Nîmes (MIPA), Université de Nîmes (UNIMES), MRI-Montpellier RIO Imaging, MOSAIC (MOSAIC), Institut FRESNEL (FRESNEL), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU), Institut des Neurosciences de Montpellier (INM), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), and Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Microscope, Laser scanning, General Chemical Engineering, Spectrum Analysis, Raman, General Biochemistry, Genetics and Molecular Biology, law.invention, Mice, 03 medical and health sciences, symbols.namesake, Optics, law, Microscopy, Aluminum Oxide, Animals, Humans, ComputingMilieux_MISCELLANEOUS, Myelin Sheath, Titanium, Microscopy, Confocal, General Immunology and Microbiology, business.industry, Lasers, General Neuroscience, Laser, Sciatic Nerve, 030104 developmental biology, Femtosecond, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Optical parametric oscillator, symbols, Raman spectroscopy, business, Raman scattering
Abstract

Laser scanning microscopes combining a femtosecond Ti:sapphire laser and an optical parametric oscillator (OPO) to duplicate the laser line have become available for biologists. These systems are primarily designed for multi-channel two-photon fluorescence microscopy. However, without any modification, complementary non-linear optical microscopy such as second-harmonic generation (SHG) or third harmonic generation (THG) can also be performed with this set-up, allowing label-free imaging of structured molecules or aqueous medium-lipid interfaces. These techniques are well suited for in-vivo observation, but are limited in chemical specificity. Chemically selective imaging can be obtained from inherent vibration signals based on Raman scattering. Confocal Raman microscopy provides 3D spatial resolution, but it requires high average power and long acquisition time. To overcome these difficulties, recent advances in laser technology have permitted the development of nonlinear optical vibrational microscopy, in particular coherent anti-Stokes Raman scattering (CARS). CARS microscopy has therefore emerged as a powerful tool for biological and live cell imaging, by chemically mapping lipids (via C-H stretch vibration), water (via O-H stretch vibrations), proteins or DNA. In this work, we describe the implementation of the CARS technique on a standard OPO-coupled multiphoton laser scanning microscope. It is based on the in-time synchronization of the two laser lines by adjusting the length of one of the laser beam path. We present a step-by-step implementation of this technique on an existing multiphoton system. A basic background in experimental optics is helpful and the presented system does not require expensive supplementary equipment. We also illustrate CARS imaging obtained on myelin sheaths of sciatic nerve of rodent, and we show that this imaging can be performed simultaneously with other nonlinear optical imaging, such as standard two-photon fluorescence technique and second-harmonic generation.

Published
2016

10. The potential of quantitative multiphoton micro(endo)scopy in regenerative medicine and tissue engineering

Author

Vytautas Kucikas, Hervé RIGNEAULT, Vasyl Mytskaniuk, Frédéric Louradour, Fabert Marc, Marc van Zandvoort, Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), MOSAIC (MOSAIC), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Universitätsklinikum RWTH Aachen - University Hospital Aachen [Aachen, Germany] (UKA), Rheinisch-Westfälische Technische Hochschule Aachen University (RWTH), and Rigneault, Herve

Subjects
[SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

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