Search

Your search keyword '"Vassallo, Melanie"' showing total 24 results
Start Over Author "Vassallo, Melanie"
24 results on '"Vassallo, Melanie"'

2. Mapping Progress: An analysis of trends in reporting race and ethnicity data in neuro-oncology clinical trials

Author

Maldonado-McGrath, Dalynah, primary, Mercurio, Ann C, additional, Abbate, Zachary, additional, Bloom, Emma, additional, Boyer, Angelique, additional, Katz-Lichtenstein, Hilla, additional, Lenchur, Peter, additional, Mezic, Matea, additional, Vassallo, Melanie, additional, Pandit, Maya, additional, Crorkin, Patrick J, additional, and Etienne, Mill, additional

Published
2024
Full Text
View/download PDF

3. CX3CL1-Fc treatment prevents atherosclerosis in Ldlr KO mice.

Author

Riopel, Matthew, Vassallo, Melanie, Ehinger, Erik, Pattison, Jennifer, Bowden, Karen, Winkels, Holger, Wilson, Maria, de Jong, Ron, Patel, Sanjay, Balakrishna, Deepika, Bilakovics, James, Fanjul, Andrea, Plonowski, Artur, Larson, Christopher J, Ley, Klaus, Cabrales, Pedro, Witztum, Joseph L, Olefsky, Jerrold M, and Lee, Yun Sok

Subjects
Aorta, Cells, Cultured, Animals, Mice, Inbred C57BL, Mice, Receptors, LDL, Recombinant Proteins, Immunoglobulin Fc Fragments, Male, Atherosclerosis, Chemokine CX3CL1, Plaque, Atherosclerotic, CX3CR1, Fractalkine, Inflammation, Ldlr KO, Monocyte adhesion, Cells, Cultured, Inbred C57BL, Receptors, LDL, Plaque, Atherosclerotic, Biochemistry and Cell Biology, Physiology
Abstract

OBJECTIVE:Atherosclerosis is a major cause of cardiovascular disease. Monocyte-endothelial cell interactions are partly mediated by expression of monocyte CX3CR1 and endothelial cell fractalkine (CX3CL1). Interrupting the interaction between this ligand-receptor pair should reduce monocyte binding to the endothelial wall and reduce atherosclerosis. We sought to reduce atherosclerosis by preventing monocyte-endothelial cell interactions through use of a long-acting CX3CR1 agonist. METHODS:In this study, the chemokine domain of CX3CL1 was fused to the mouse Fc region to generate a long-acting soluble form of CX3CL1 suitable for chronic studies. CX3CL1-Fc or saline was injected twice a week (30 mg/kg) for 4 months into Ldlr knockout (KO) mice on an atherogenic western diet. RESULTS:CX3CL1-Fc-treated Ldlr KO mice showed decreased en face aortic lesion surface area and reduced aortic root lesion size with decreased necrotic core area. Flow cytometry analyses of CX3CL1-Fc-treated aortic wall cell digests revealed a decrease in M1-like polarized macrophages and T cells. Moreover, CX3CL1-Fc administration reduced diet-induced atherosclerosis after switching from an atherogenic to a normal chow diet. In vitro monocyte adhesion studies revealed that CX3CL1-Fc treatment caused fewer monocytes to adhere to a human umbilical vein endothelial cell monolayer. Furthermore, a dorsal window chamber model demonstrated that CX3CL1-Fc treatment decreased in vivo leukocyte adhesion and rolling in live capillaries after short-term ischemia-reperfusion. CONCLUSION:These results indicate that CX3CL1-Fc can inhibit monocyte/endothelial cell adhesion as well as reduce atherosclerosis.

Published
2019

4. Regulatory CD4+ T Cells Recognize Major Histocompatibility Complex Class II Molecule–Restricted Peptide Epitopes of Apolipoprotein B

Author

Kimura, Takayuki, Kobiyama, Kouji, Winkels, Holger, Tse, Kevin, Miller, Jacqueline, Vassallo, Melanie, Wolf, Dennis, Ryden, Christian, Orecchioni, Marco, Dileepan, Thamotharampillai, Jenkins, Marc K, James, Eddie A, Kwok, William W, Hanna, David B, Kaplan, Robert C, Strickler, Howard D, Durkin, Helen G, Kassaye, Seble G, Karim, Roksana, Tien, Phyllis C, Landay, Alan L, Gange, Stephen J, Sidney, John, Sette, Alessandro, and Ley, Klaus

Subjects
Biomedical and Clinical Sciences, Immunology, Immunization, Cardiovascular, Prevention, Vaccine Related, Atherosclerosis, Heart Disease, 2.1 Biological and endogenous factors, Aetiology, Good Health and Well Being, Adjuvants, Immunologic, Animals, Aorta, Aortic Diseases, Apolipoprotein B-100, Apolipoproteins B, Disease Models, Animal, Epitope Mapping, Epitopes, T-Lymphocyte, Female, Freund's Adjuvant, Histocompatibility Antigens Class II, Humans, Lymphocyte Activation, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, ApoE, Peptide Fragments, Plaque, Atherosclerotic, T-Lymphocytes, Regulatory, Vaccination, antigen specificity, apoB-100, atherosclerosis, regulatory T cells, vaccination, Cardiorespiratory Medicine and Haematology, Clinical Sciences, Public Health and Health Services, Cardiovascular System & Hematology, Cardiovascular medicine and haematology, Clinical sciences, Sports science and exercise
Abstract

BackgroundCD4+ T cells play an important role in atherosclerosis, but their antigen specificity is poorly understood. Immunization with apolipoprotein B (ApoB, core protein of low density lipoprotein) is known to be atheroprotective in animal models. Here, we report on a human APOB peptide, p18, that is sequence-identical in mouse ApoB and binds to both mouse and human major histocompatibility complex class II molecules.MethodsWe constructed p18 tetramers to detect human and mouse APOB-specific T cells and assayed their phenotype by flow cytometry including CD4 lineage transcription factors, intracellular cytokines, and T cell receptor activation. Apolipoprotein E-deficient ( Apoe-/-) mice were vaccinated with p18 peptide or adjuvants alone, and atherosclerotic burden in the aorta was determined.ResultsIn human peripheral blood mononuclear cells from donors without cardiovascular disease, p18 specific CD4+ T cells detected by a new human leukocyte antigen-antigen D related-p18 tetramers were mostly Foxp3+ regulatory T cells (Tregs). Donors with subclinical cardiovascular disease as detected by carotid artery ultrasound had Tregs coexpressing retinoic acid-related orphan receptor gamma t or T-bet, which were both almost absent in donors without cardiovascular disease. In Apoe-/- mice, immunization with p18 induced Tregs and reduced atherosclerotic lesions. After peptide restimulation, responding CD4+ T cells identified by Nur77-GFP (green fluorescent protein) were highly enriched in Tregs. A new mouse I-Ab-p18 tetramer identified the expansion of p18-specific CD4+ T cells on vaccination, which were enriched for interleukin-10-producing Tregs.ConclusionsThese findings show that APOB p18-specific CD4+ T cells are mainly Tregs in healthy donors, but coexpress other CD4 lineage transcription factors in donors with subclinical cardiovascular disease. This study identifies ApoB peptide 18 as the first Treg epitope in human and mouse atherosclerosis.

Published
2018

5. Pathogenic Autoimmunity in Atherosclerosis Evolves From Initially Protective Apolipoprotein B100–Reactive CD4+ T-Regulatory Cells

Author

Wolf, Dennis, Gerhardt, Teresa, Winkels, Holger, Michel, Nathaly Anto, Pramod, Akula Bala, Ghosheh, Yanal, Brunel, Simon, Buscher, Konrad, Miller, Jacqueline, McArdle, Sara, Baas, Livia, Kobiyama, Kouji, Vassallo, Melanie, Ehinger, Erik, Dileepan, Thamotharampillai, Ali, Amal, Schell, Maximilian, Mikulski, Zbigniew, Sidler, Daniel, Kimura, Takayuki, Sheng, Xia, Horstmann, Hauke, Hansen, Sophie, Mitre, Lucia Sol, Stachon, Peter, Hilgendorf, Ingo, Gaddis, Dalia E., Hedrick, Catherine, Benedict, Chris A., Peters, Bjoern, Zirlik, Andreas, Sette, Alessandro, and Ley, Klaus

Published
2020
Full Text
View/download PDF

6. Atlas of the Immune Cell Repertoire in Mouse Atherosclerosis Defined by Single-Cell RNA-Sequencing and Mass Cytometry

Author

Winkels, Holger, Ehinger, Erik, Vassallo, Melanie, Buscher, Konrad, Dinh, Huy Q., Kobiyama, Kouji, Hamers, Anouk A.J., Cochain, Clément, Vafadarnejad, Ehsan, Saliba, Antoine-Emmanuel, Zernecke, Alma, Pramod, Akula Bala, Ghosh, Amlan K., Anto Michel, Nathaly, Hoppe, Natalie, Hilgendorf, Ingo, Zirlik, Andreas, Hedrick, Catherine C., Ley, Klaus, and Wolf, Dennis

Published
2018
Full Text
View/download PDF

8. Autoimmune Regulator (AIRE) Deficiency Does Not Affect Atherosclerosis and CD4 T Cell Immune Tolerance to Apolipoprotein B

Author

Nettersheim, Felix Sebastian, Braumann, Simon, Kobiyama, Kouji, Orecchioni, Marco, Vassallo, Melanie, Miller, Jacqueline, Ali, Amal, Roy, Payel, Saigusa, Ryosuke, Wolf, Dennis, Ley, Klaus, Winkels, Holger, Nettersheim, Felix Sebastian, Braumann, Simon, Kobiyama, Kouji, Orecchioni, Marco, Vassallo, Melanie, Miller, Jacqueline, Ali, Amal, Roy, Payel, Saigusa, Ryosuke, Wolf, Dennis, Ley, Klaus, and Winkels, Holger

Abstract

Atherosclerosis is a chronic, lipid-driven disease of medium sized arteries which causes myocardial infarction and stroke. Recently, an adaptive immune response against the plaque-associated autoantigen Apolipoprotein B100 (ApoB), the structural protein component of low-density lipoprotein, has been implicated in atherogenesis. In healthy individuals, CD4(+) T cells responding to ApoB mainly comprised regulatory T cells, which confer immune tolerance and atheroprotection. Mice and patients with atherosclerosis harbor increased numbers of proatherogenic ApoB-reactive T-helper cell subsets. Given the lack of therapies targeting proatherogenic immunity, clarification of the underlying mechanisms is of high clinical relevance. T cells develop in the thymus, where strong autoreactive T cells are eliminated in the process of negative selection. Herein, we investigated whether the transcription factor autoimmune regulator (AIRE), which controls expression of numerous tissue-restricted self-antigens in the thymus, is involved in mediating tolerance to ApoB and whether Aire deficiency might contribute to atherogenesis. Mice deficient for Aire were crossbred to apolipoprotein E-deficient mice to obtain atherosclerosis-prone Aire(-/-) Apoe(-/-) mice, which were fed a regular chow diet (CD) or western-type diet (WD). CD4(+) T cells responding to the ApoB peptide p6 were analyzed by flow cytometry. We demonstrate that Aire deficiency influences neither generation nor activation of ApoB-reactive T cells and has only minor and overall inconsistent impacts on their phenotype. Furthermore, we show that atherosclerotic plaque size is not affected in Aire(-/-) Apoe(-/-) compared to Aire(+/+) Apoe(-/-), irrespective of diet and gender. In conclusion, our data suggests that AIRE is not involved in regulating thymic expression of ApoB or atherosclerosis. Alternative mechanisms how ApoB-reactive CD4 T cells are selected in the thymus will have to be investigated.

Published
2022

9. Autoimmune Regulator (AIRE) Deficiency Does Not Affect Atherosclerosis and CD4 T Cell Immune Tolerance to Apolipoprotein B

Author

Nettersheim, Felix Sebastian, primary, Braumann, Simon, additional, Kobiyama, Kouji, additional, Orecchioni, Marco, additional, Vassallo, Melanie, additional, Miller, Jacqueline, additional, Ali, Amal, additional, Roy, Payel, additional, Saigusa, Ryosuke, additional, Wolf, Dennis, additional, Ley, Klaus, additional, and Winkels, Holger, additional

Published
2022
Full Text
View/download PDF

10. Pathogenic Autoimmunity in Atherosclerosis Evolves From Initially Protective Apolipoprotein B 100 –Reactive CD4 + T-Regulatory Cells

Author

Wolf, Dennis, primary, Gerhardt, Teresa, additional, Winkels, Holger, additional, Michel, Nathaly Anto, additional, Pramod, Akula Bala, additional, Ghosheh, Yanal, additional, Brunel, Simon, additional, Buscher, Konrad, additional, Miller, Jacqueline, additional, McArdle, Sara, additional, Baas, Livia, additional, Kobiyama, Kouji, additional, Vassallo, Melanie, additional, Ehinger, Erik, additional, Dileepan, Thamotharampillai, additional, Ali, Amal, additional, Schell, Maximilian, additional, Mikulski, Zbigniew, additional, Sidler, Daniel, additional, Kimura, Takayuki, additional, Sheng, Xia, additional, Horstmann, Hauke, additional, Hansen, Sophie, additional, Mitre, Lucia Sol, additional, Stachon, Peter, additional, Hilgendorf, Ingo, additional, Gaddis, Dalia E., additional, Hedrick, Catherine, additional, Benedict, Chris A., additional, Peters, Bjoern, additional, Zirlik, Andreas, additional, Sette, Alessandro, additional, and Ley, Klaus, additional

Published
2020
Full Text
View/download PDF

11. Regulatory CD4(+) T cells recognize MHC-II-restricted peptide epitopes of apolipoprotein B

Author

Kimura, Takayuki, Kobiyama, Kouji, Winkels, Holger, Tse, Kevin, Miller, Jacqueline, Vassallo, Melanie, Wolf, Dennis, Ryden, Christian, Orecchioni, Marco, Dileepan, Thamotharampillai, Jenkins, Marc K., James, Eddie A., Kwok, William W., Hanna, David B., Kaplan, Robert C., Strickler, Howard D., Durkin, Helen G., Kassaye, Seble G., Karim, Roksana, Tien, Phyllis C., Landay, Alan L., Gange, Stephen J., Sidney, John, Sette, Alessandro, Biol.Sci., and Ley, Klaus

Subjects
Male, endocrine system, endocrine system diseases, Mice, Knockout, ApoE, Freund's Adjuvant, Aortic Diseases, Epitopes, T-Lymphocyte, chemical and pharmacologic phenomena, Lymphocyte Activation, T-Lymphocytes, Regulatory, Article, Mice, Adjuvants, Immunologic, Animals, Humans, Aorta, Apolipoproteins B, Vaccination, Histocompatibility Antigens Class II, Atherosclerosis, Peptide Fragments, Plaque, Atherosclerotic, Mice, Inbred C57BL, Disease Models, Animal, Apolipoprotein B-100, Female, Epitope Mapping
Abstract

BACKGROUND: CD4(+) T cells play an important role in atherosclerosis, but their antigen specificity is poorly understood. Immunization with apolipoprotein B (ApoB, core protein of low density lipoprotein) is known to be atheroprotective in animal models. Here, we report on a human APOB peptide, p18, that is sequence-identical in mouse ApoB and binds to both mouse and human MHC-II. METHODS: We constructed p18-tetramers to detect human and mouse APOB-specific T cells and assayed their phenotype by flow cytometry including CD4 lineage transcription factors, intracellular cytokines, and TCR activation. Apoe(−/−) mice were vaccinated with p18 peptide or adjuvants alone and atherosclerotic burden in the aorta was determined. RESULTS: In human peripheral blood mononuclear cells from donors without cardiovascular disease (CVD), p18 specific CD4(+) T cells detected by a new HLA-DR-p18 tetramers were mostly Foxp3(+) regulatory T cells (Tregs). Donors with subclinical CVD as detected by carotid artery ultrasound had Tregs co-expressing RORγt or T-bet which were both almost absent in donors without CVD. In Apoe(−/−) mice, immunization with p18 induced Tregs and reduced atherosclerotic lesions. After peptide restimulation, responding CD4(+) T cells identified by Nur77-GFP were highly enriched in Tregs. A new mouse I-A(b)-p18 tetramer identified the expansion of p18-specific CD4(+) T cells upon vaccination, which were enriched for IL-10-producing Tregs. CONCLUSION: These findings show that APOB p18 specific CD4(+) T cells are mainly Tregs in healthy donors, but co-express other CD4 lineage transcription factors in donors with subclinical CVD. This study identifies ApoB peptide 18 as the first Treg epitope in human and mouse atherosclerosis.

Published
2018

12. A clinically applicable adjuvant for an atherosclerosis vaccine

Author

Kobiyama, Kouji, Vassallo, Melanie, Mitzi, Jessica, Winkels, Holger, Pei, Hong, Kimura, Takayuki, Miller, Jacqueline, Wolf, Dennis, and Ley, Klaus

Subjects
CD4-Positive T-Lymphocytes, Mice, Knockout, Squalene, Vaccines, Freund's Adjuvant, Vaccination, Polysorbates, Atherosclerosis, Lipids, Article, Mice, Adjuvants, Immunologic, Immunoglobulin G, Animals, Female, Apolipoproteins B
Abstract

Vaccination with MHC-II-restricted peptides from Apolipoprotein B (ApoB) with complete and incomplete Freund's adjuvant (CFA/IFA) is known to protect mice from atherosclerosis. This vaccination induces antigen-specific IgG1 and IgG2c antibody responses and a robust CD4 T cell response in lymph nodes. However, CFA/IFA cannot be used in humans. To find a clinically applicable adjuvant, we tested the effect of vaccinating Apoe-deficient mice with ApoB peptide P6 (TGAYSNASSTESASY). In a broad screening experiment, Addavax, a squalene-based oil-in-water adjuvant similar to MF59, was the only adjuvant that showed similar efficacy as CFA/IFA. This was confirmed in a confirmation experiment for both the aortic arch and whole aorta analyzed by en face analysis after atherosclerotic lesion staining. Mechanistically, restimulated peritoneal cells from mice immunized with P6 in Addavax released significant amounts of IL-10. Unlike P6 in CFA/IFA, vaccination with P6 in Addavax did not induce any detectable IgG1 or IgG2c antibodies to P6. These data suggest that squalene-based adjuvants such as MF59 are good candidate adjuvants for developing a clinically effective atherosclerosis vaccine.

Published
2018

13. CX3CL1-Fc treatment prevents atherosclerosis in Ldlr KO mice

Author

Riopel, Matthew, primary, Vassallo, Melanie, additional, Ehinger, Erik, additional, Pattison, Jennifer, additional, Bowden, Karen, additional, Winkels, Holger, additional, Wilson, Maria, additional, de Jong, Ron, additional, Patel, Sanjay, additional, Balakrishna, Deepika, additional, Bilakovics, James, additional, Fanjul, Andrea, additional, Plonowski, Artur, additional, Larson, Christopher J., additional, Ley, Klaus, additional, Cabrales, Pedro, additional, Witztum, Joseph L., additional, Olefsky, Jerrold M., additional, and Lee, Yun Sok, additional

Published
2019
Full Text
View/download PDF

14. Pathogenic Autoimmunity in Atherosclerosis Evolves From Initially Protective Apolipoprotein B100-Reactive CD4+ T-Regulatory Cells.

Author

Wolf, Dennis, Gerhardt, Teresa, Winkels, Holger, Michel, Nathaly Anto, Pramod, Akula Bala, Ghosheh, Yanal, Brunel, Simon, Buscher, Konrad, Miller, Jacqueline, McArdle, Sara, Baas, Livia, Kobiyama, Kouji, Vassallo, Melanie, Ehinger, Erik, Dileepan, Thamotharampillai, Ali, Amal, Schell, Maximilian, Mikulski, Zbigniew, Sidler, Daniel, and Kimura, Takayuki

Published
2020
Full Text
View/download PDF

15. Regulatory CD4 + T Cells Recognize Major Histocompatibility Complex Class II Molecule–Restricted Peptide Epitopes of Apolipoprotein B

Author

Kimura, Takayuki, primary, Kobiyama, Kouji, additional, Winkels, Holger, additional, Tse, Kevin, additional, Miller, Jacqueline, additional, Vassallo, Melanie, additional, Wolf, Dennis, additional, Ryden, Christian, additional, Orecchioni, Marco, additional, Dileepan, Thamotharampillai, additional, Jenkins, Marc K., additional, James, Eddie A., additional, Kwok, William W., additional, Hanna, David B., additional, Kaplan, Robert C., additional, Strickler, Howard D., additional, Durkin, Helen G., additional, Kassaye, Seble G., additional, Karim, Roksana, additional, Tien, Phyllis C., additional, Landay, Alan L., additional, Gange, Stephen J., additional, Sidney, John, additional, Sette, Alessandro, additional, and Ley, Klaus, additional

Published
2018
Full Text
View/download PDF

17. Comprehensive assessment of immune cells in mouse and human atherosclerosis by single cell RNA sequencing and mass cytometry

Author

Winkels, Holger, primary, Ehinger, Erik, additional, Vassallo, Melanie, additional, Buscher, Konrad, additional, Dinh, Huy, additional, Kobiyama, Kouji, additional, Hamers, Anouk, additional, Cochain, Clement, additional, Vafadarnejad, Ehsan, additional, Saliba, Antoine Emmanuel, additional, Zernecke, Alma, additional, Bala, Pramod Akula, additional, Ghosh, Amlan, additional, Michel, Nathaly Anto, additional, Hoppe, Natalie, additional, Hilgendorf, Ingo, additional, Zirlik, Andreas, additional, Hedrick, Catherine, additional, Ley, Klaus, additional, and Wolf, Dennis, additional

Published
2018
Full Text
View/download PDF

19. MHC class II tetramers identify apolipoprotein B-specific CD4 T cells in mice and humans

Author

Kimura, Takayuki, primary, Tse, Kevin, additional, Miller, Jacqueline, additional, McArdle, Sara, additional, Vassallo, Melanie, additional, Wolf, Dennis, additional, Baas, Livia, additional, Kobiyama, Koji, additional, Jenkins, Marc K, additional, James, Eddie A, additional, Kwok, William, additional, Hanna, David B, additional, Kaplan, Robert C, additional, Strickler, Howard, additional, Sidney, John, additional, Sette, Alessandro, additional, and Ley, Klaus, additional

Published
2017
Full Text
View/download PDF

20. Pathogenic Autoimmunity in Atherosclerosis Evolves From Initially Protective Apolipoprotein B100–Reactive CD4+T-Regulatory Cells

Author

Wolf, Dennis, Gerhardt, Teresa, Winkels, Holger, Michel, Nathaly Anto, Pramod, Akula Bala, Ghosheh, Yanal, Brunel, Simon, Buscher, Konrad, Miller, Jacqueline, McArdle, Sara, Baas, Livia, Kobiyama, Kouji, Vassallo, Melanie, Ehinger, Erik, Dileepan, Thamotharampillai, Ali, Amal, Schell, Maximilian, Mikulski, Zbigniew, Sidler, Daniel, Kimura, Takayuki, Sheng, Xia, Horstmann, Hauke, Hansen, Sophie, Mitre, Lucia Sol, Stachon, Peter, Hilgendorf, Ingo, Gaddis, Dalia E., Hedrick, Catherine, Benedict, Chris A., Peters, Bjoern, Zirlik, Andreas, Sette, Alessandro, and Ley, Klaus

Abstract

Supplemental Digital Content is available in the text.

Published
2020
Full Text
View/download PDF

21. Regulatory CD4+T Cells Recognize Major Histocompatibility Complex Class II Molecule–Restricted Peptide Epitopes of Apolipoprotein B

Author

Kimura, Takayuki, Kobiyama, Kouji, Winkels, Holger, Tse, Kevin, Miller, Jacqueline, Vassallo, Melanie, Wolf, Dennis, Ryden, Christian, Orecchioni, Marco, Dileepan, Thamotharampillai, Jenkins, Marc K., James, Eddie A., Kwok, William W., Hanna, David B., Kaplan, Robert C., Strickler, Howard D., Durkin, Helen G., Kassaye, Seble G., Karim, Roksana, Tien, Phyllis C., Landay, Alan L., Gange, Stephen J., Sidney, John, Sette, Alessandro, and Ley, Klaus

Abstract

Supplemental Digital Content is available in the text.

Published
2018
Full Text
View/download PDF

22. Regulatory CD4+ T Cells Recognize Major Histocompatibility Complex Class II Molecule-Restricted Peptide Epitopes of Apolipoprotein B.

Author

Kimura, Takayuki, Kobiyama, Kouji, Winkels, Holger, Tse, Kevin, Miller, Jacqueline, Vassallo, Melanie, Wolf, Dennis, Ryden, Christian, Orecchioni, Marco, Dileepan, Thamotharampillai, Jenkins, Marc K, James, Eddie A, Kwok, William W, Hanna, David B, Kaplan, Robert C, Strickler, Howard D, Durkin, Helen G, Kassaye, Seble G, Karim, Roksana, and Tien, Phyllis C

Published
2018
Full Text
View/download PDF

23. Readers Weight In on ‘Big Boxes’ And Service With a Smile.

Author

Huntington, Carol, Roberts, John, Strong, Gail, Pike, Jane, Hill, Karen A., Dolan, Allison F., and Vassallo, Melanie

Abstract

Presents letters to the editor in response to article discussing plants retailing in the U.S. published in the previous issue of "People Places & Plants."

Published
2004

24. GUIDELINES FOR GIVING A GREAT LECTURE.

Author

Vassallo, Melanie D.

Abstract

Presents guidelines for giving a lecture. Use of a laser pointer; Purpose of pausing after the change of slides; Ways to overcome a stage fright; Advice on dealing with a disappointment.

Published
2005

Catalog

Books, media, physical & digital resources
See catalog results