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5. Forecasting the relative abundance of Aedes vector populations to enhance situational awareness for mosquito control operations.

Author

Ventura, Paulo C., Kummer, Allisandra G., Wilke, André B. B., Chitturi, Jagadeesh, Hill, Megan D., Vasquez, Chalmers, Unlu, Isik, Mutebi, John-Paul, Kluh, Susanne, Vetrone, Steve, Damian, Dan, Townsend, John, Litvinova, Maria, and Ajelli, Marco

Abstract

Aedes-borne diseases represent a major public health threat and mosquito control operations represent a key line of defense. Improving the real-time awareness of mosquito control authorities by providing reliable forecasts of the relative abundance of mosquito vectors could greatly enhance control efforts. To this aim, we developed an analytical tool that forecasts Aedes aegypti relative abundance 1 to 4 weeks ahead. Forecasts were validated against mosquito surveillance data (2,760 data points) collected over multiple years in four jurisdictions in the US. The symmetric absolute percentage error was in the range 0.43–0.69, and the 90% interquantile range of the forecasts had a coverage of 83–92%. Our forecasts consistently outperformed a reference "naïve" model for all analyzed study sites, forecasting horizon, and for periods with medium/high Ae. aegypti activity. The developed tool can be instrumental to address the need for evidence-based decision making. Author summary: Mosquito control authorities have faced major emergencies, but they lacked access to analytical tools for guiding the planning of mosquito control strategies and outbreak response efforts. This deficiency has not only impeded their situational awareness but has also hindered their ability to make informed decisions regarding intervention planning to respond to Aedes-borne disease outbreaks. Our study addresses this need by developing an analytical tool that forecasts Aedes aegypti relative abundance 1 to 4 weeks ahead. Our forecasts were consistently accurate and precise for all analyzed study sites, forecasting horizon, and for periods with medium/high Ae. Aegypti activity. Our results indicate that our analytical tool can be used to guide mosquito control operations, ultimately reducing the risk of Aedes-borne disease outbreaks. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Diel activity patterns of two distinct populations of Aedes aegypti in Miami, FL and Brownsville, TX

Author

Mutebi, John-Paul, Wilke, André Barretto Bruno, Ostrum, Erik, Vasquez, Chalmers, Cardenas, Gabriel, Carvajal, Augusto, Moreno, Maday, Petrie, William D., Rodriguez, Arturo, Presas, Henry, Rodriguez, Jesus, Barnes, Fred, Hamer, Gabriel L., Juarez, Jose G., Carbajal, Ester, Vitek, Christopher J., Estrada, Xochitl, Rios, Thalia, Marshall, Jeremy, and Beier, John C.

Published
2022
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8. Global genomics of Aedes aegypti unveils widespread and novel persistent infectious viruses capable of triggering a small RNA response

Author

Gupta, Shruti, primary, Sharma, Rohit, additional, Williams, Adeline E., additional, Sanchez-Vargas, Irma, additional, Rose, Noah H., additional, Zhang, Chao, additional, Crosbie-Villaseca, Alexander, additional, Zhu, Zheng, additional, Dayama, Gargi, additional, Gloria-Soria, Andrea, additional, Brackney, Doug E., additional, Manning, Jessica, additional, Wheeler, Sarah S., additional, Caranci, Angela, additional, Reyes, Trinidad, additional, Sylla, Massamba, additional, Badolo, Athanase, additional, Akorli, Jewelna, additional, Aribodor, Ogechukwu B., additional, Ayala, Diego, additional, Liu, Wei-Liang, additional, Chen, Chun-Hong, additional, Vasquez, Chalmers, additional, Acosta, Cassandra Gonzalez, additional, Ponlawat, Alongkot, additional, Magalhaes, Tereza, additional, Carter, Brendan, additional, Wesson, Dawn, additional, Surin, Darred, additional, Younger, Meg A., additional, Costa-da-Silva, Andre Luis, additional, DeGennaro, Matthew, additional, Bergman, Alexander, additional, Lambrechts, Louis, additional, McBride, Carolyn S., additional, Olson, Ken E., additional, Calvo, Eric, additional, and Lau, Nelson C., additional

Published
2024
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9. Comparing the effectiveness of adulticide application interventions on mitigating local transmission of dengue virus

Author

Kummer, Allisandra G., primary, Wilke, André B.B., additional, Ventura, Paulo C., additional, Vasquez, Chalmers, additional, Medina, Johana, additional, Unlu, Isik, additional, Gonzalez, Yaziri, additional, Mhlanga, Adequate, additional, Benelli, Giovanni, additional, Ejima, Keisuke, additional, Mutebi, John-Paul, additional, and Ajelli, Marco, additional

Published
2024
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10. Mosquito Control Activities during Local Transmission of Zika Virus, Miami-Dade County, Florida, USA, 2016

Author

McAllister, Janet C., Porcelli, Mario, Medina, Johana M., Delorey, Mark J., Connelly, C. Roxanne, Godsey, Marvin S., Panella, Nicholas A., Dzuris, Nicole, Boegler, Karen A., Kenney, Joan L., Kothera, Linda, Vizcaino, Lucrecia, Lenhart, Audrey E., Mutebi, John-Paul, and Vasquez, Chalmers

Subjects
United States. Centers for Disease Control and Prevention -- Inspection, Permethrin, Mosquito control, Zika virus, Genetic vectors, Time, Containers, Pregnant women, Health
Abstract

Zika virus (ZIKV), a flavivirus that can cause birth defects and is associated with Guillain-Barre syndrome, has rapidly spread throughout the Western Hemisphere (1-3). The virus is spread primarily by [...]

Published
2020
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17. Insecticide resistance of Miami-Dade Culex quinquefasciatus populations and initial field efficacy of a new resistance-breaking adulticide formulation.

Author

Unlu, Isik, Buckner, Eva A., Medina, Johanna, Vasquez, Chalmers, Cabrera, Aimee, Romero-Weaver, Ana L., Ramirez, Daviela, Kendziorski, Natalie L., Kosinski, Kyle J., Fedirko, T. J., Ketelsen, Leigh, Dorsainvil, Chelsea, and Estep, Alden S.

Subjects
CULEX quinquefasciatus, WEST Nile virus, SUBURBS, PYRETHROIDS, DELTAMETHRIN
Abstract

Sporadic outbreaks of human cases of West Nile virus (WNV), primarily vectored by Culex quinquefasciatus Say in suburban and urban areas, have been reported since introduction of the virus into Florida in 2001. Miami-Dade County, Florida is part of one of the largest metropolitan areas in the United States, supports Cx. quinquefasciatus year-round, and recently experienced over 60 human cases of WNV during one outbreak. To facilitate more effective integrated vector management and public health protection, we used the Centers for Disease Control and Prevention (CDC) bottle bioassay method to evaluate the susceptibility of adult Cx. quinquefasciatus collected from 29 locations throughout Miami-Dade County to pyrethroid and organophosphate adulticide active ingredients (AIs) used by Miami-Dade County Mosquito Control. We also determined the frequency of the 1014 knockdown resistance (kdr) mutation for Cx. quinquefasciatus from a subset of 17 locations. We detected resistance to two pyrethroid AIs in all tested locations (permethrin: 27 locations, deltamethrin: 28 locations). The 1014F allele was widely distributed throughout all 17 locations sampled; however, 29.4% of these locations lacked 1014F homozygotes even though phenotypic pyrethroid resistance was present. Organophosphate resistance was more variable; 20.7% of the locations tested were susceptible to malathion, and 33.3% of the populations were susceptible to naled. We subsequently conducted a field trial of ReMoa Tri, a recently approved multiple AI adulticide formulation labelled for resistant mosquitoes, against a mixed location field population of Miami-Dade Cx. quinquefasciatus. Average 24-hr mortality was 65.1 ± 7.2% and 48-hr mortality increased to 85.3 ± 9.1%, indicating good control of these resistant Cx. quinquefasciatus. This current study shows that insecticide resistance is common in local Cx. quinquefasciatus but effective options are available to maintain control during active disease transmission in Miami-Dade County. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Local Mosquito-Borne Transmission of Zika Virus — Miami-Dade and Broward Counties, Florida, June–August 2016

Author

Likos, Anna, Griffin, Isabel, Bingham, Andrea M., Stanek, Danielle, Fischer, Marc, White, Stephen, Hamilton, Janet, Eisenstein, Leah, Atrubin, David, Mulay, Prakash, Scott, Blake, Jenkins, Patrick, Fernandez, Danielle, Rico, Edhelene, Gillis, Leah, Jean, Reynald, Cone, Marshall, Blackmore, Carina, McAllister, Janet, Vasquez, Chalmers, Rivera, Lillian, and Philip, Celeste

Published
2016

21. Diel activity patterns of vector mosquito species in the urban environment: Implications for vector control strategies

Author

Wilke, André B. B., primary, Mhlanga, Adequate, additional, Kummer, Allisandra G., additional, Vasquez, Chalmers, additional, Moreno, Maday, additional, Petrie, William D., additional, Rodriguez, Art, additional, Vitek, Christopher, additional, Hamer, Gabriel L., additional, Mutebi, John-Paul, additional, and Ajelli, Marco, additional

Published
2023
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24. Diel activity patterns of vector mosquito species in the urban environment: Implications for vector control strategies

Author

Wilke, André B. B., primary, Mhlanga, Adequate, additional, Kummer, Allisandra G., additional, Vasquez, Chalmers, additional, Moreno, Maday, additional, Petrie, William D., additional, Rodriguez, Art, additional, Vitek, Christopher, additional, Hamer, Gabriel L., additional, Mutebi, John-Paul, additional, and Ajelli, Marco, additional

Published
2022
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27. Diel Activity Patterns of Two Distinct Populations of Aedes Aegypti in Miami, FL and Brownsville, TX

Author

Mutebi, John-Paul, primary, Wilke, André Barretto Bruno, additional, Ostrum, Erik, additional, Vasquez, Chalmers, additional, Cardenas, Gabriel, additional, Carvajal, Augusto, additional, Moreno, Maday, additional, Petrie, William D., additional, Rodriguez, Arturo, additional, Presas, Henry, additional, Rodriguez, Jesus, additional, Barnes, Fred, additional, Hamer, Gabriel L., additional, Juarez, Jose, additional, Vitek, Christopher J., additional, and Beier, John C., additional

Published
2021
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29. A molecular surveillance-guided vector control response to concurrent dengue and West Nile virus outbreaks in a COVID-19 hotspot of Florida

Author

Coatsworth, Heather, primary, Lippi, Catherine A., additional, Vasquez, Chalmers, additional, Ayers, Jasmine B., additional, Stephenson, Caroline J., additional, Waits, Christy, additional, Florez, Mary, additional, Wilke, André B. B., additional, Unlu, Isik, additional, Medina, Johana, additional, Alcaide, Maria L., additional, Ryan, Sadie J., additional, Lednicky, John A., additional, Beier, John C., additional, Petrie, William, additional, and Dinglasan, Rhoel R., additional

Published
2021
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31. Zika virus evolution and spread in the Americas

Author

Metsky, Hayden C., Matranga, Christian B., Wohl, Shirlee, Schaffner, Stephen F., Freije, Catherine A., Winnicki, Sarah M., West, Kendra, Qu, James, Baniecki, Mary Lynn, Gladden-Young, Adrianne, Lin, Aaron E., Tomkins-Tinch, Christopher H., Ye, Simon H., Park, Daniel J., Luo, Cynthia Y., Barnes, Kayla G., Shah, Rickey R., Chak, Bridget, Barbosa-Lima, Giselle, Delatorre, Edson, Vieira, Yasmine R., Paul, Lauren M., Tan, Amanda L., Barcellona, Carolyn M., Porcelli, Mario C., Vasquez, Chalmers, Cannons, Andrew C., Cone, Marshall R., Hogan, Kelly N., Kopp, Edgar W., Anzinger, Joshua J., Garcia, Kimberly F., Parham, Leda A., Ramrez, Rosa M. Glvez, Montoya, Maria C. Miranda, Rojas, Diana P., Brown, Catherine M., Hennigan, Scott, Sabina, Brandon, Scotland, Sarah, Gangavarapu, Karthik, Grubaugh, Nathan D., Oliveira, Glenn, Robles-Sikisaka, Refugio, Rambaut, Andrew, Gehrke, Lee, Smole, Sandra, Halloran, M. Elizabeth, Villar, Luis, Mattar, Salim, Lorenzana, Ivette, Cerbino-Neto, Jose, Valim, Clarissa, Degrave, Wim, Bozza, Patricia T., Gnirke, Andreas, Andersen, Kristian G., Isern, Sharon, Michael, Scott F., Bozza, Fernando A., Souza, Thiago M. L., Bosch, Irene, Yozwiak, Nathan L., MacInnis, Bronwyn L., and Sabeti, Pardis C.

Subjects
America -- Health aspects, Zika virus -- Natural history, Zika virus infection -- Distribution, Company distribution practices, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Hayden C. Metsky [1, 2]; Christian B. Matranga [1]; Shirlee Wohl [1, 3]; Stephen F. Schaffner [1, 3, 4]; Catherine A. Freije [1, 3]; Sarah M. Winnicki [1]; Kendra [...]

Published
2017
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32. Genomic epidemiology reveals multiple introductions of Zika virus into the United States

Author

Grubaugh, Nathan D., Ladner, Jason T., Kraemer, Moritz U. G., Dudas, Gytis, Tan, Amanda L., Gangavarapu, Karthik, Wiley, Michael R., White, Stephen, Thz, Julien, Magnani, Diogo M., Prieto, Karla, Reyes, Daniel, Bingham, Andrea M., Paul, Lauren M., Robles-Sikisaka, Refugio, Oliveira, Glenn, Pronty, Darryl, Barcellona, Carolyn M., Metsky, Hayden C., Baniecki, Mary Lynn, Barnes, Kayla G., Chak, Bridget, Freije, Catherine A., Gladden-Young, Adrianne, Gnirke, Andreas, Luo, Cynthia, MacInnis, Bronwyn, Matranga, Christian B., Park, Daniel J., Qu, James, Schaffner, Stephen F., Tomkins-Tinch, Christopher, West, Kendra L., Winnicki, Sarah M., Wohl, Shirlee, Yozwiak, Nathan L., Quick, Joshua, Fauver, Joseph R., Khan, Kamran, Brent, Shannon E., Reiner, Robert C., Jr, Lichtenberger, Paola N., Ricciardi, Michael J., Bailey, Varian K., Watkins, David I., Cone, Marshall R., Kopp, Edgar W., IV, Hogan, Kelly N., Cannons, Andrew C., Jean, Reynald, Monaghan, Andrew J., Garry, Robert F., Loman, Nicholas J., Faria, Nuno R., Porcelli, Mario C., Vasquez, Chalmers, Nagle, Elyse R., Cummings, Derek A. T., Stanek, Danielle, Rambaut, Andrew, Sanchez-Lockhart, Mariano, Sabeti, Pardis C., Gillis, Leah D., Michael, Scott F., Bedford, Trevor, Pybus, Oliver G., Isern, Sharon, Palacios, Gustavo, and Andersen, Kristian G.

Subjects
Zika virus -- Health aspects -- Genetic aspects, Gene expression -- Health aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Nathan D. Grubaugh [1]; Jason T. Ladner [2]; Moritz U. G. Kraemer [3, 4, 5]; Gytis Dudas [6]; Amanda L. Tan [7]; Karthik Gangavarapu [1]; Michael R. Wiley [2, [...]

Published
2017
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33. Modelling distributions of Aedes aegypti and Aedes albopictus using climate, host density and interspecies competition

Author

Yang, Bingyi, primary, Borgert, Brooke A., additional, Alto, Barry W., additional, Boohene, Carl K., additional, Brew, Joe, additional, Deutsch, Kelly, additional, DeValerio, James T., additional, Dinglasan, Rhoel R., additional, Dixon, Daniel, additional, Faella, Joseph M., additional, Fisher-Grainger, Sandra L., additional, Glass, Gregory E., additional, Hayes, Reginald, additional, Hoel, David F., additional, Horton, Austin, additional, Janusauskaite, Agne, additional, Kellner, Bill, additional, Kraemer, Moritz U. G., additional, Lucas, Keira J., additional, Medina, Johana, additional, Morreale, Rachel, additional, Petrie, William, additional, Reiner, Robert C., additional, Riles, Michael T., additional, Salje, Henrik, additional, Smith, David L., additional, Smith, John P., additional, Solis, Amy, additional, Stuck, Jason, additional, Vasquez, Chalmers, additional, Williams, Katie F., additional, Xue, Rui-De, additional, and Cummings, Derek A. T., additional

Published
2021
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41. Assessment of the effectiveness of BG-Sentinel traps baited with CO2 and BG-Lure for the surveillance of vector mosquitoes in Miami-Dade County, Florida

Author

Wilke, André B. B., primary, Carvajal, Augusto, additional, Medina, Johana, additional, Anderson, Melissa, additional, Nieves, Veronica J., additional, Ramirez, Monica, additional, Vasquez, Chalmers, additional, Petrie, William, additional, Cardenas, Gabriel, additional, and Beier, John C., additional

Published
2019
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42. Genome sequencing reveals Zika virus diversity and spread in the Americas

Author

Metsky, Hayden C, Matranga, Christian B, Wohl, Shirlee, Schaffner, Stephen F., Freije, Catherine A, Winnicki, Sarah, West, Kendra, Qu, J, Baniecki, Mary Lynn, Gladden-Young, Adrianne, Lin, Aaron E., Christopher, Tomkins-Tinch, Ye, S.H, Park, Daniel J, Luo, Cynthia, Barnes, Kayla G, Shah, R.R., Chak, Bridget, Barbosa-Lima, G., Delatorre, E., Vieira, Y.R., Paul, Lauren M, Tan, Amanda L, Barcellona, Carolyn M, Porcelli, Mario C, Vasquez, Chalmers, Cannons, Andrew C, Cone, Marshall R, Hogan, Kelly N, Kopp IV, Edgar W, Anzinger, J.J., Garcia, K.F., Parhap, L.A., Gelvez Ramirez, R.M., Montoya, Miranda, Rojas, D.P., Brown, C.M., Hennigan, S., Sabina, B., Scotland, S., Gangavarapu, K., Grubaugh, N.D., Oliveira, G., Robles-Sikisaka, R., Rambaut, Andrew, Gehrke, L., Smole, S., Halloran, M.E., Villar Centeno, L.A., Mattar, S., Lorenzana, I., Cerbino-Neto, J., Valim, C., Degrave, W., Bozza, P.T., Souza, T.M.L., Bosch, I., Yozwiak, N.L., MacInnis, B.L., and Sabeti, P.C.

Abstract

Despite great attention given to the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects1,2, much remains unknown about ZIKV disease epidemiology and ZIKV evolution, in part due to a lack of genomic data. We applied multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analyzed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental US. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of viral surveillance. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those potentially relevant to the effectiveness of diagnostic tests.

Published
2017
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44. Modelling the distribution of Aedes aegypti and Aedes albopictus using climate, host density and interspecies competitive effects

Author

Yang, Bingyi, primary, Borgert, Brooke A., additional, Alto, Barry W., additional, Boohene, Carl K., additional, Brabant, Peter, additional, Brew, Joe, additional, Deutsch, Kelly, additional, DeValerio, James T., additional, Dinglasan, Rhoel R., additional, Dixon, Daniel, additional, Faella, Joseph M., additional, Fisher-Grainger, Sandra L., additional, Glass, Gregory E., additional, Hayes, Reginald, additional, Hoel, David F., additional, Horton, Austin, additional, Janusauskaite, Agne, additional, Kellner, Bill, additional, Kraemer, Moritz U.G., additional, Leveen, Eric, additional, Lucas, Keira J., additional, Medina, Johana, additional, Morreale, Rachel, additional, Petrie, William, additional, Reiner, Robert C., additional, Riles, Michael T., additional, Salje, Henrik, additional, Smith, David L., additional, Smith, John P., additional, Solis, Amy, additional, Stuck, Jason, additional, Vasquez, Chalmers, additional, Williams, Katie F., additional, Xue, Rui-De, additional, and Cummings, Derek A.T., additional

Published
2018
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46. Zika virus evolution and spread in the Americas

Author

Institute for Medical Engineering and Science, Broad Institute of MIT and Harvard, Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Metsky, Hayden C., Schaffner, Stephen F, Quigley, James Edwin, Ye, Simon Huang, Park, Daniel, Barnes, Kayla, Gehrke, Lee, Valim, Clarissa, Gnirke, Andreas, Andersen, Kristian, Bosch, Irene, Matranga, Christian B., Wohl, Shirlee, Freije, Catherine A., Winnicki, Sarah M., West, Kendra, Baniecki, Mary Lynn, Gladden-Young, Adrianne, Lin, Aaron E., Tomkins-Tinch, Christopher H., Luo, Cynthia Y., Shah, Rickey R., Chak, Bridget, Barbosa-Lima, Giselle, Delatorre, Edson, Vieira, Yasmine R., Paul, Lauren M., Tan, Amanda L., Barcellona, Carolyn M., Porcelli, Mario C., Vasquez, Chalmers, Cannons, Andrew C., Cone, Marshall R., Hogan, Kelly N., Kopp, Edgar W., Anzinger, Joshua J., Garcia, Kimberly F., Parham, Leda A., Ramírez, Rosa M. Gélvez, Montoya, Maria C. Miranda, Rojas, Diana P., Brown, Catherine M., Hennigan, Scott, Sabina, Brandon, Scotland, Sarah, Gangavarapu, Karthik, Grubaugh, Nathan D., Oliveira, Glenn, Robles-Sikisaka, Refugio, Rambaut, Andrew, Smole, Sandra, Halloran, M. Elizabeth, Villar, Luis, Mattar, Salim, Lorenzana, Ivette, Cerbino-Neto, Jose, Degrave, Wim, Bozza, Patricia T., Isern, Sharon, Michael, Scott F., Bozza, Fernando A., Souza, Thiago M. L., Yozwiak, Nathan L., MacInnis, Bronwyn L., Sabeti, Pardis C., Institute for Medical Engineering and Science, Broad Institute of MIT and Harvard, Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Metsky, Hayden C., Schaffner, Stephen F, Quigley, James Edwin, Ye, Simon Huang, Park, Daniel, Barnes, Kayla, Gehrke, Lee, Valim, Clarissa, Gnirke, Andreas, Andersen, Kristian, Bosch, Irene, Matranga, Christian B., Wohl, Shirlee, Freije, Catherine A., Winnicki, Sarah M., West, Kendra, Baniecki, Mary Lynn, Gladden-Young, Adrianne, Lin, Aaron E., Tomkins-Tinch, Christopher H., Luo, Cynthia Y., Shah, Rickey R., Chak, Bridget, Barbosa-Lima, Giselle, Delatorre, Edson, Vieira, Yasmine R., Paul, Lauren M., Tan, Amanda L., Barcellona, Carolyn M., Porcelli, Mario C., Vasquez, Chalmers, Cannons, Andrew C., Cone, Marshall R., Hogan, Kelly N., Kopp, Edgar W., Anzinger, Joshua J., Garcia, Kimberly F., Parham, Leda A., Ramírez, Rosa M. Gélvez, Montoya, Maria C. Miranda, Rojas, Diana P., Brown, Catherine M., Hennigan, Scott, Sabina, Brandon, Scotland, Sarah, Gangavarapu, Karthik, Grubaugh, Nathan D., Oliveira, Glenn, Robles-Sikisaka, Refugio, Rambaut, Andrew, Smole, Sandra, Halloran, M. Elizabeth, Villar, Luis, Mattar, Salim, Lorenzana, Ivette, Cerbino-Neto, Jose, Degrave, Wim, Bozza, Patricia T., Isern, Sharon, Michael, Scott F., Bozza, Fernando A., Souza, Thiago M. L., Yozwiak, Nathan L., MacInnis, Bronwyn L., and Sabeti, Pardis C.

Abstract

Although the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects have attracted a great deal of attention1,2, much remains unknown about ZIKV disease epidemiology and ZIKV evolution, in part owing to a lack of genomic data. Here we address this gap in knowledge by using multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analysed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental United States. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of surveillance of viral infections. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those that might be relevant to the effectiveness of diagnostic tests., National Institutes of Health (U.S.) (Grant NIAID U19AI110818)

Published
2017

47. Establishment of Aedes(Ochlerotatus) scapularis(Diptera: Culicidae) in Mainland Florida, With Notes on the Ochlerotatus Group in the United States

Author

Reeves, Lawrence E, Medina, Johana, Miqueli, Evaristo, Sloyer, Kristin E, Petrie, William, Vasquez, Chalmers, and Burkett-Cadena, Nathan D

Abstract

Aedes scapularis(Rondani), a widespread neotropical vector mosquito species, has been included in the mosquito fauna of Florida on the basis of just three larval specimens that were collected in the middle Florida Keys in 1945. Here, we report numerous recent collections of immature and adult Ae. scapularisfrom multiple locations in two counties of southern Florida. These specimens represent the first records of Ae. scapularisfrom mainland Florida and the first records of the species in the state since the initial detection of the species 75 yr ago. Collections of both larvae and adults across several years indicate that Ae. scapularisis now established in Broward and Miami-Dade Counties. These contemporary records of this species in Florida may represent novel dispersal and subsequent establishment events from populations outside the United States or a recent reemergence of undetected endemic populations. To confirm morphological identification of Ae. scapularisspecimens from Florida, the DNA barcoding region of the cytochrome coxidase subunit I gene (COI) was sequenced and compared to all other Ochlerotatus Group species from the United States, specifically Aedes condolescensDyar and Knab (Diptera: Culicidae), Aedes infirmatusDyar and Knab (Diptera: Culicidae), Aedes thelcterDyar (Diptera: Culicidae), Aedes tortilis(Theobald) (Diptera: Culicidae), and Aedes trivittatus(Coquillett) (Diptera: Culicidae). Molecular assays and sequencing confirm morphological identification of Ae. scapularisspecimens. Maximum likelihood phylogenetic analysis of COI and ITS2 sequences place Florida Ae. scapularisin a distinct clade, but was unable to produce distinct clades for Florida specimens of Ae. condolescensand Ae. tortilis.Graphical Abstract

Published
2021
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48. Multiple introductions of Zika virus into the United States revealed through genomic epidemiology

Author

Grubaugh, Nathan D, primary, Ladner, Jason T, additional, Kraemer, Moritz UG, additional, Dudas, Gytis, additional, Tan, Amanda L, additional, Gangavarapu, Karthik, additional, Wiley, Michael R, additional, White, Stephen, additional, Thézé, Julien, additional, Magnani, Diogo M, additional, Prieto, Karla, additional, Reyes, Daniel, additional, Bingham, Andrea, additional, Paul, Lauren M, additional, Robles-Sikisaka, Refugio, additional, Oliveira, Glenn, additional, Pronty, Darryl, additional, Metsky, Hayden C, additional, Baniecki, Mary Lynn, additional, Barnes, Kayla G, additional, Chak, Bridget, additional, Freije, Catherine A, additional, Gladden-Young, Adrianne, additional, Gnirke, Andreas, additional, Luo, Cynthia, additional, MacInnis, Bronwyn, additional, Matranga, Christian B, additional, Park, Daniel J, additional, Qu, James, additional, Schaffner, Stephen F, additional, Tomkins-Tinch, Christopher, additional, West, Kendra L, additional, Winnicki, Sarah M, additional, Wohl, Shirlee, additional, Yozwiak, Nathan L, additional, Quick, Joshua, additional, Fauver, Joseph R, additional, Khan, Kamran, additional, Brent, Shannon E, additional, Reiner, Robert C, additional, Lichtenberger, Paola N, additional, Ricciardi, Michael, additional, Bailey, Varian K, additional, Watkins, David I, additional, Cone, Marshall R, additional, Kopp, Edgar W, additional, Hogan, Kelly N, additional, Cannons, Andrew C, additional, Jean, Reynald, additional, Garry, Robert F, additional, Loman, Nicholas J, additional, Faria, Nuno R, additional, Porcelli, Mario C, additional, Vasquez, Chalmers, additional, Nagle, Elyse R, additional, Cummings, Derek AT, additional, Stanek, Danielle, additional, Rambaut, Andrew, additional, Sanchez-Lockhart, Mariano, additional, Sabeti, Pardis C, additional, Gillis, Leah D, additional, Michael, Scott F, additional, Bedford, Trevor, additional, Pybus, Oliver G, additional, Isern, Sharon, additional, Palacios, Gustavo, additional, and Andersen, Kristian G, additional

Published
2017
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49. Assessment of the effectiveness of BG-Sentinel traps baited with CO2 and BG-Lure for the surveillance of vector mosquitoes in Miami-Dade County, Florida.

Author

Wilke, André B. B., Carvajal, Augusto, Medina, Johana, Anderson, Melissa, Nieves, Veronica J., Ramirez, Monica, Vasquez, Chalmers, Petrie, William, Cardenas, Gabriel, and Beier, John C.

Subjects
MOSQUITOES, CULEX quinquefasciatus, SPECIES diversity, GENERALIZED estimating equations
Abstract

Vector-borne diseases are an increasing issue to public health, endangering billions of people worldwide. Controlling vector mosquitoes is widely accepted as the most effective way to prevent vector-borne disease outbreaks. Mosquito surveillance is critical for the development of control strategies under the integrated vector management framework. We hypothesize that the effectiveness and reliability of using BG-Sentinel traps for the surveillance strongly depend on the bait used to attract mosquitoes. The objective of this study was to compare the effectiveness of BG-Sentinel traps baited with CO2 and BG-Lure. A total of 72 traps were deployed for 48 hours once a week for four weeks. For the initial 24-hour period, the traps were baited with CO2, and then for an additional 24 hours using the BG-Lure. Collected mosquitoes were analyzed using the Generalized Estimating Equation for repeated measures analysis. Biodiversity was assessed by the Shannon and Simpson indices and by individual rarefaction curves and SHE profiles. A total of 5,154 mosquitoes were collected, from which 3,514 by traps baited with CO2 and 1,640 mosquitoes by traps baited with BG-Lure. Aedes aegypti and Culex quinquefasciatus were the most abundant and dominant species. Results from the Generalized Estimating Equation models indicated that more than twice as many mosquitoes were attracted CO2 than to the BG-Lure. The comparison of attractiveness of CO2 and BG-Lure to Ae. aegypti and Cx. quinquefasciatus was non-significant, suggesting that both species were equally attracted by the baits. The individual rarefaction curves for Ae. aegypti and Cx. quinquefasciatus imply that traps baited with BG-Lure underestimated mosquito species richness compared to those baited with CO2. BG-Lure were less effective in attracting mosquitoes with low abundances and failed to collect Cx. coronator and Cx. nigripalpus, which were consistently collected by traps baited with CO2. According to our results, CO2 significantly (P<0.05) attracted more mosquitoes (2.67 adjusted odds ratios) than the BG-Lure when adjusted for time and species, being more effective in assessing the relative abundance of vector mosquitoes and yielding more trustworthy results. Traps baited with CO2 collected not only more specimens, but also more species in a more consistent pattern. [ABSTRACT FROM AUTHOR]

Published
2019
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