Search

BackgroundAccess to water and sanitation is a basic human right; however, in many parts of the world, communities experience water, sanitation, and hygiene (WaSH) insecurity. While WaSH insecurity is prevalent in many low and middle-income countries, it is also a problem in high-income countries, like the United States, as is evident in vulnerable populations, including people experiencing homelessness. Limited knowledge exists about the coping strategies unhoused people use to access WaSH services. This study, therefore, examines WaSH access among unhoused communities in Los Angeles, California, a city with the second-highest count of unhoused people across the nation.MethodsWe conducted a cross-sectional study using a snowball sampling technique with 263 unhoused people living in Skid Row, Los Angeles. We calculated frequencies and used multivariable models to describe (1) how unhoused communities cope and gain access to WaSH services in different places, and (2) what individual-level factors contribute to unhoused people's ability to access WaSH services.ResultsOur findings reveal that access to WaSH services for unhoused communities in Los Angeles is most difficult at night. Reduced access to overnight sanitation resulted in 19% of the sample population using buckets inside their tents and 28% openly defecating in public spaces. Bottled water and public taps are the primary drinking water source, but 6% of the sample reported obtaining water from fire hydrants, and 50% of the population stores water for night use. Unhoused people also had limited access to water and soap for hand hygiene throughout the day, with 17% of the sample relying on hand sanitizer to clean their hands. Shower and laundry access were among the most limited services available, and reduced people's ability to maintain body hygiene practices and limited employment opportunities. Our regression models suggest that WaSH access is not homogenous among the unhoused. Community differences exist; the odds of having difficulty accessing sanitation services is two times greater for those living outside of Skid Row (Adj OR: 2.52; 95% CI: 1.08-6.37) and three times greater for people who have been unhoused for more than six years compared to people who have been unhoused for less than a year (Adj OR: 3.26; 95% CI: 1.36-8.07).ConclusionOverall, this study suggests a need for more permanent, 24-h access to WaSH services for unhoused communities living in Skid Row, including toilets, drinking water, water and soap for hand hygiene, showers, and laundry services.

Language treatment for bilinguals with aphasia has been shown to result in gains in both the treated language and the untreated language (i.e., cross-language generalization). However, cross-language generalization is not consistently found. This inconsistency may be due to several factors, such as the age of acquisition of, and proficiency in, each language. One often-overlooked factor that may influence whether cross-language generalization occurs is the manner in which bilinguals learned their second language (L2): in a formal educational context (explicitly) or naturalistically through exposure to the language (implicitly). Prior research suggests that implicit L2 learning results in greater overlap in the representation of the first language (L1) and L2 in the brain, particularly for grammar, compared to explicit learning. In contrast, lexical processing in L1 and L2 is proposed to rely more on shared brain regions regardless of the manner of L2 acquisition. Greater overlap should provide a greater likelihood of cross-language generalization effects from treatment. The goal of this study was to determine how the manner of acquisition of L2 may affect cross-language generalization following treatment in L2 separately targeting the lexicon (object naming) and grammar (sentence construction). Two Spanish-English bilinguals with aphasia each completed two treatment phases in English of 2-4 weeks each, in succession, with one week between them: semantic feature analysis (SFA) targeting object naming, and Verb Network Strengthening Treatment (VNeST) targeting verb retrieval and sentence construction. Pre- and post-treatment assessments and weekly probes were completed for each phase. Participant P1 learned English explicitly in an educational setting, while participant P2 learned English implicitly. As predicted, P2 showed cross-language generalization after verb/grammar treatment (VNeST) whereas P1 did not. However, contrary to the prediction that both participants should show cross-language generalization after noun treatment (SFA), only P1 showed cross-language generalization of object naming. Cross-language generalization was observed for both participants but for different aspects of language. The findings suggest that naturalistic second language learning may lead to stronger links between languages in the grammatical system, whereas formal second language learning may lead to stronger links between languages in the lexical-semantic system. Future research should further explore the effects of manner of acquisition as a predictor of language co-activation and cross-language treatment generalization in bilinguals with aphasia. [ABSTRACT FROM AUTHOR]

Loss of auditory hair cells (deafness) is the major sensory disability. Mammalian hair cells neither repair nor regenerate, but hearing restoration by adenoviral gene therapy of cochlear supporting cells was recently proven. Math-1 transfection using a non-viral polymeric, magnetically susceptible nanoparticle vector is this study goal. Superparamagnetic iron oxide nanoparticles (SPION) coated with oleic acid, were encapsulated into poly(D,Llactide-co-glycolide) (PLGA). Particle size (dynamic light scattering) varied from 160 nm to 280 nm while zeta potential remained near -20 mV. Transmission electron microscopy showed SPION uniformly encapsulated in PLGA with diameters of 5 to 15 nm. PLGA-SPION composite nanoparticles were pulled by external magnetic field and gradient through the round window membrane of guinea pigs and cadavers, and into various cell cultures, confirmed by transmission electron or confocal microscopy. When nanoparticles were infused into the cochlea, particles also were found in the organ of Corti. Magnetic targeting resulted in particles delivered to the round window membrane, perilymph and cochlear basal turn (basilar membrane, and stria vascularis). The results suggest nanoparticle non-viral vectors can deliver Math-1 and therapeutic biomolecules into the cochlea across the round window membrane using an external magnetic field. [ABSTRACT FROM AUTHOR]