Search

Your search keyword '"Vasily V. Unguryan"' showing total 2 results
Start Over Author "Vasily V. Unguryan"
2 results on '"Vasily V. Unguryan"'

1. Re-Emergence of Circulation of Seasonal Influenza during COVID-19 Pandemic in Russia and Receptor Specificity of New and Dominant Clade 3C.2a1b.2a.2 A(H3N2) Viruses in 2021–2022

Author

Natalia P. Kolosova, Tatiana N. Ilyicheva, Vasily V. Unguryan, Alexey V. Danilenko, Svetlana V. Svyatchenko, Galina S. Onhonova, Natalia I. Goncharova, Maksim N. Kosenko, Andrey S. Gudymo, Vasiliy Y. Marchenko, Alexander N. Shvalov, Ivan M. Susloparov, Tatiana V. Tregubchak, Elena V. Gavrilova, Rinat A. Maksyutov, and Alexander B. Ryzhikov

Subjects
seasonal influenza virus, COVID-19 pandemic influence, monitoring, molecular modeling, A(H3N2) clade 3C.2a1b.2a.2, receptor specificity, Medicine
Abstract

The circulation of seasonal influenza in 2020–2021 around the world was drastically reduced after the start of the COVID-19 pandemic and the implementation of mitigation strategies. The influenza virus circulation reemerged in 2021–2022 with the global spread of the new genetic clade 3C.2a1b.2a.2 of A(H3N2) viruses. The purpose of this study was to characterize influenza viruses in the 2021–2022 season in Russia and to analyze the receptor specificity properties of the 3C.2a1b.2a.2 A(H3N2) viruses. Clinical influenza samples were collected at the local Sanitary-and-Epidemiological Centers of Rospotrebnadzor. Whole genome sequencing was performed using NGS. The receptor specificity of hemagglutinin was evaluated using molecular modeling and bio-layer interferometry. Clinical samples from 854 cases of influenza A and B were studied; A(H3N2) viruses were in the majority of the samples. All genetically studied A(H3N2) viruses belonged to the new genetic clade 3C.2a1b.2a.2. Molecular modeling analysis suggested a higher affinity of hemagglutinin of 3C.2a1b.2a.2. A(H3N2) viruses to the α2,6 human receptor. In vitro analysis using a trisaccharide 6’-Sialyl-N-acetyllactosamine receptor analog did not resolve the differences in the receptor specificity of 3C.2a1b.2a.2 clade viruses from viruses belonging to the 3C.2a1b.2a.1 clade. Further investigation of the A(H3N2) viruses is required for the evaluation of their possible adaptive advantages. Constant monitoring and characterization of influenza are critical for epidemiological analysis.

Published
2022
Full Text
View/download PDF

2. Double Electron-Electron Resonance of Spin-Labeled Cholestane in Model Membranes: Evidence for Substructures inside the Lipid Rafts

Author

Sergei A. Dzuba, Elena A. Golysheva, and Vasily V. Unguryan

Subjects
Cholestanes, Superlattice, Lipid Bilayers, Electron Spin Resonance Spectroscopy, Resonance, Electrons, Raft, Surfaces, Coatings and Films, Crystallography, chemistry.chemical_compound, Membrane, Membrane Microdomains, chemistry, Phase (matter), Materials Chemistry, Phosphatidylcholines, Molecule, Cholestane, Spin Labels, Physical and Theoretical Chemistry, Lipid raft
Abstract

Plasma membranes are assumed to be highly compartmentalized, which is believed to be important for the membrane protein functionality. The liquid ordered-disordered phase segregation in the membranes results in nanoscale liquid-ordered assemblies-lipid rafts. Double electron-electron resonance spectroscopy (DEER, also known as PELDOR) is sensitive to spin-spin dipolar interactions between spin labels at the nanoscale range of distances. Here, DEER is applied to spin-labeled cholestane, 3β-doxyl-5α-cholestane (DChl), diluted in bilayers composed of an equimolar mixture of dioleoyl-glycero-phosphocholine (DOPC) and dipalmitoyl-glycero-phosphocholine (DPPC) phospholipids, with cholesterol (Chol) added. The DEER data allowed us to detect clustering of the DChl molecules. Their lateral distribution in the clusters in the absence of Chol was found to be random, while in the presence of Chol it became quasi-regular. DEER time traces are fairly well simulated within a simple square superlattice model. For the 20 mol % Chol content, for which at physiological temperatures, the lipid rafts are formed, the found superlattice parameter was 3.7 nm. Assuming that lipid rafts are captioned upon shock freezing at the temperature of investigation (80 K), the found regularity of DChl lateral distribution was interpreted by raft substructuring, with the DChl molecules embedded between the substructures.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results