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Follow-ups of 39 children aged 3 to 12 years who had various biomedical risk factors of local forms of primary tuberculosis. Difficulties in diagnosing the local clinical forms of primary tuberculosis with minor clinical and X-ray signs are shown. The authors show it necessary to use of a package of clinical, laboratory, and radiation techniques, by applying computed tomography. The latter excluded minor primary tuberculosis in 15.3+/-6.2% of children and confirmed an active tuberculous process in 23+/-6.7%, and referred it as an incomplete regression phase in 10+/-4.8% and assigned residual changes of spontaneous cure of primary tuberculosis in 9 (23+/-6.7%). There was agreement of these conventional radiation studies and computed tomography in 11 (28.12+/-6.2%) children.

On the basis of X-ray determined coordinates specified with computer graphics and new data on amino acids sequence in the structure of Bacillus intermedius 7P ribonuclease there were analysed potentially charged residues and found ionic pairs binding independently denaturing regions-energetic domains. Five clusters of charged amino acids were found in the protein structure and several ionic bonds between the energetic domains were discovered: Asp7, Asp11-Arg109 and Lys26-Asp53,Glu72. Breaking of the latter makes their heat denaturation independent. Local surrounding of the ionic bonds is characterized and their role in the structure and cooperative properties of the globule is discussed.

The crystals of free cytosolic chicken aspartate aminotransferase were subjected to X-ray investigation at 2.7 A. One subunit of the dimeric molecule crystalline enzyme is in the open conformation and the other is in the closed conformation.

The molecular and crystal structures of the antiviral compound, (S)-9-(2,3-dihydroxypropyl)adenine, was established. The space group is P21, unit-cell parameters a 5,546(1), b 8,381(1), c 10,119(1), beta 91,979(9) degrees, Z2. The structure was solved by the direct method and refined by a full-matrix least-squares procedure to R 4,2%. All non-hydrogen atoms of this compound are concentrated in two planes. The first one involves the atoms of the purine moiety and N(6) and C(1'), while the second one accommodates C(2'), C(3'), O(2') and O(3'). The angle between these planes is 54,3 degrees. The conformation of the compound in crystal was compared with that deduced from theoretical analysis.