1. Hepatoprotective Effect of Essential Oils from Hyptis crenata in Sepsis-Induced Liver Dysfunction.
- Author
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Lima GC, Vasconcelos YAG, de Santana Souza MT, Oliveira AS, Bomfim RR, de Albuquerque Júnior RLC, Camargo EA, Portella VG, Coelho-de-Souza AN, and Diniz LRL
- Subjects
- Alanine Transaminase genetics, Alanine Transaminase metabolism, Animals, Aspartate Aminotransferases genetics, Aspartate Aminotransferases metabolism, Brazil, Catalase genetics, Catalase metabolism, Humans, Liver drug effects, Liver enzymology, Liver metabolism, Liver Diseases etiology, Liver Diseases genetics, Liver Diseases metabolism, Male, Malondialdehyde metabolism, Oxidative Stress drug effects, Rats, Rats, Wistar, Superoxide Dismutase genetics, Superoxide Dismutase metabolism, Hyptis chemistry, Liver Diseases drug therapy, Oils, Volatile administration & dosage, Plant Extracts administration & dosage, Protective Agents administration & dosage, Sepsis complications
- Abstract
No specific therapeutics are available for the treatment of sepsis-induced liver dysfunction, a clinical complication strongly associated with the high mortality rate of septic patients. This study investigated the effect of the essential oil of Hyptis crenata (EOHc), a lamiaceae plant used to treat liver disturbances in Brazilian folk medicine, on liver function during early sepsis. Sepsis was induced by the cecal ligation and puncture (CLP) model. Rats were divided into four groups: Sham, Sham+EOHc, CLP, and CLP+EOHc. EOHc (300 mg/kg) was orally administered 12 and 24 h after surgery. The animals were sacrificed for blood collection and liver tissue samples 48 h after surgery. Hepatic function was evaluated by measuring serum bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase, and alanine aminotransferase (ALT) levels. The levels of malondialdehyde and the activity of superoxide dismutase, catalase, and GSH peroxidase (GSH-Px) were measured for assessment of oxidative stress. Liver morphology was analyzed by hematoxylin and eosin staining. EOHc normalized serum ALP, ALT, and bilirubin levels and inhibited morphological changes. In addition, we observed that EOHc inhibited elevation in hepatic lipid peroxidation and reduction of the glutathione peroxidase activity induced by sepsis. Our data show that EOHc plays a protective effect against liver injury induced by sepsis.
- Published
- 2018
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