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Felid herpesvirus type 1 (FHV-1)-associated dermatitis is characterized by facial and nasal involvement; clinical and histopathological manifestations may overlap with other dermatitides.To evaluate the realibility of qRT-PCR-2Sixteen FFPE samples from cats with facial dermatitis and four controls were studied.Based on histopathological features, cases were separated into: Group 1, samples with herpetic dermatitis (four); Group 2, samples with nonherpetic facial dermatitis (six); Group 3, samples with facial dermatitis of ambiguous nature (allergic or viral) (six); and Group 4, samples from healthy cats (four). A relative quantification using the 2By 2QRT-PCR 2La dermatite liée à herpesvirus félin de type 1 (FHV-1) est caractérisée par des lésions faciles et nasales ; les manifestations cliniques et histopathologiques peuvent se superposer à d'autres dermatoses.Evaluer la capacité des méthodes qRT-PCR-2Seize échantillons de FFPE de chats avec dermatite faciale et quatre contrôles ont été étudiés. MATÉRIELS ET MÉTHODES: A partir des données histopathologiques, les cas ont été séparés en deux groupes : Groupe 1, échantillons avec dermatite herpétique (quatre) ; Groupe 2, échantillons avec dermatite faciale non herpétique (six) ; Groupe 3, échantillons avec dermatite faciale de nature ambiguë (allergique ou virale) (six) ; et Groupe 4, échantillons de chats sains (quatre). Une quantification relative à l'aide de la méthode 2Les analyses QRT-PCR 2INTRODUCCIÓN: la dermatitis asociada al herpesvirus tipo 1 (FHV-1) se caracteriza por afectación facial y nasal; Las manifestaciones clínicas e histopatológicas pueden superponerse con otras dermatitis. OBJETIVO: evaluar la viabilidad de los métodos de hibridación in situ qRT-PCR-2Die feline Herpesvirus Typ 1 (FHV-1) assoziierte Dermatitis wird charakterisiert durch Veränderungen im Gesicht und an der Nase; klinische und histopathologische Manifestationen könnten mit anderen Dermatitiden überlappen. ZIEL: Eine Evaluierung der Verlässlichkeit von qRT-PCR-2Sechzehn FFPE Proben von Katzen mit einer Dermatitis des Gesichts und vier Kontrollen wurden untersucht.Die Fälle wurden anhand der histopathologischen Merkmale folgendermaßen in Gruppen unterteilt: Gruppe 1, Proben mit Herpesdermatitis (vier); Gruppe 2, Proben mit Nicht-Herpes-bedingter Dermatitis des Gesichts (sechs); Gruppe 3, Proben von Gesichtsdermatitis unterschiedlicher Natur (allergisch oder viral)(sechs); und Gruppe 4, Proben von gesunden Katzen (vier). Eine relative Quantifizierung mittels 2Mittels 2Die QRT-PCR 2背景: ネコヘルペスウイルス1型(FHV-1)関連皮膚炎は、顔面および鼻病変が特徴的である。臨床症状および組織病理学的兆候は、他の皮膚炎と重複する可能性がある。 目的: 本研究の目的は、ホルマリン固定パラフィン包埋(FFPE)組織においてFHV-1関連皮膚炎を診断するqRT-PCR-2背景: 猫I型疱疹病毒(FHV-1)相关皮炎的特征是面部和鼻部出现病变;临床和组织病理学表现可能与其他皮炎存在交叉。 目的: 在福尔马林固定、石蜡包埋(FFPE)组织中,用qRT-PCR-2A dermatite associada a herpesvírus felino tipo 1 (HVF-1) é caracterizada por acometimento facial e nasal; as manifestações clínicas e histopatológicas podem se confundir com outras dermatopatias.Avaliar a confiabilidade dos métodos qRT-PCR-2Foram estudadas dezesseis amostras de pele fixadas em formol e parafinadas de gatos com dermatite facial e quatro controles. MÉTODOS E MATERIAIS: Baseado nas características histopatológicas, os casos foram divididos em: Grupo 1, amostras com dermatite herpética (quatro); Grupo 2, amostras com dermatites não herpéticas (seis); Grupo 3, amostras com dermatite facial de natureza ambígua (alérgica ou viral) (seis); e Grupo 4, amostras dos gatos saudáveis (quatro). Utilizou-se uma quantificação relativa através do método 2A partir da análise 2

The present study is a CFD investigation of the gasification behaviour of different of difficult coals at high pressure in the siemens 5-MW piolot-scale entrained-flow reactor. The detailed models for char conversion kinetics are adjusted based on laboratory investigations of the same coals and then coupled to the CFD framework for the full-scale simulations. The gasifiction reactions in entrained-flow reacteors predominantly take place in the so-called pore-diffusion regime. In order to correctly predict the carbon conversion, suitable models for intrinsic and pore evolution are required for each feedstock under the operating conditions considerded. In this work, the char gasification kinetics of the investigated coals are first characterized in separate laboratory-scale experiments for the kinetic and pore diffusion regimes. This data is used to calibrate the char conversion models, which are then coupled to the CFD code. This comprehensive modelling framework is finally used to simulate the conversion behaviour of the coals in the measured carbon conversions under different operating conditions (equivalence rations) and the syngas composition after quench. The analysis carried out in this work demonstrates that accurate that accurate predictions of carbon conversion in industrial-scale gasifiers can be obtained using advanced intrinsic char gasification models when calibrated with laboratory-scale experiments.