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7. First report of Tobacco necrosis virus D infecting tomato in Greece.

Author

Beris, D., Galeou, A., Kektsidou, O., and Varveri, C.

Subjects
TOMATOES, NECROSIS, TOBACCO, NUCLEOTIDE sequencing, CUCUMBERS
Abstract

This article reports the first case of Tobacco necrosis virus D (TNV-D) infecting tomatoes in Greece. The virus was identified through RNA sequencing and confirmed through RT-PCR. TNV-D is a member of the Betanecrovirus genus and is known to infect a wide range of plant species. While it is not a direct threat to tomato cultivation, further investigation is needed to understand its spread, epidemiology, and impact on tomato crops. This research was funded by the European Union. [Extracted from the article]

Published
2024
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13. First report of Ti ringspot‐associated virus infecting peach in Greece.

Author

Beris, D., Malandraki, I., and Varveri, C.

Abstract

This article reports the first occurrence of Ti ringspot-associated virus (TiRSaV) infecting peach trees in Greece. The virus was detected in a sample of clingstone peach exhibiting virus-like symptoms, such as mosaic and chlorotic spots on the leaves, bud failure, and severe fruit deformity. High Throughput Sequencing (HTS) was used to analyze the sample, and the presence of TiRSaV was confirmed through de novo assembly and BLASTn analysis. This finding expands the known host range of TiRSaV and suggests the need for further investigation into the potential risks it may pose to cultivated plants. [Extracted from the article]

Published
2024
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15. High-throughput sequencing technologies for plant pest diagnosis: challenges and opportunities

Author

Olmos, A., Boonham, N., Candresse, T., Gentit, P., Giovani, B., Kutnjak, D., Liefting, L., Maree, H.J., Minafra, A., Moreira, A., Nakhla, M.K., Petter, F., Ravnikar, M., Rodoni, B.C., Roenhorst, J.W., Rott, M., Ruiz-Garcia, A.B., Santala, J., Stancanelli, G., van der Vlugt, R.A.A., Varveri, C., Westenberg, M., Wetzel, T., Ziebell, H., Massart, S., Olmos, A., Boonham, N., Candresse, T., Gentit, P., Giovani, B., Kutnjak, D., Liefting, L., Maree, H.J., Minafra, A., Moreira, A., Nakhla, M.K., Petter, F., Ravnikar, M., Rodoni, B.C., Roenhorst, J.W., Rott, M., Ruiz-Garcia, A.B., Santala, J., Stancanelli, G., van der Vlugt, R.A.A., Varveri, C., Westenberg, M., Wetzel, T., Ziebell, H., and Massart, S.

Abstract

High‐throughput sequencing (HTS) technologies have revolutionized plant pest research and are now raising interest for plant pest diagnostics, with plant virus diagnostics at the forefront of development. However, the application of HTS in plant pest diagnostics raises important challenges that plant health regulators will have to address. Adapted infrastructures, technical guidelines and training are pivotal for further use and adoption of the HTS technologies in the phytosanitary framework.

Published
2018

16. High-throughput sequencing technologies for plant pest diagnosis: challenges and opportunities

Author

Olmos, A., primary, Boonham, N., additional, Candresse, T., additional, Gentit, P., additional, Giovani, B., additional, Kutnjak, D., additional, Liefting, L., additional, Maree, H.J., additional, Minafra, A., additional, Moreira, A., additional, Nakhla, M.K., additional, Petter, F., additional, Ravnikar, M., additional, Rodoni, B., additional, Roenhorst, J.W., additional, Rott, M., additional, Ruiz‐García, A.B., additional, Santala, J., additional, Stancanelli, G., additional, van der Vlugt, R., additional, Varveri, C., additional, Westenberg, M., additional, Wetzel, T., additional, Ziebell, H., additional, and Massart, S., additional

Published
2018
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17. Host range and symptomatology of Pepino mosaic virus strains occurring in Europe

Author

Ravnikar, M., Blystad, D.R., van der Vlugt, R., Alfaro Fernández, Ana Olvido, del Carmen Cordoba, M, Bese, G., Hristova, D., Pospieszny, H., Mehle, N., Ravnikar, Maja, Tomassoli, L, Varveri, C., and Nielsen, S.L.

Subjects
Veterinary medicine, Laboratory of Virology, Strains, Plant Science, Horticulture, Nicotiana occidentalis, potex virus, strains, Laboratorium voor Virologie, test plants, Bioint Diagnostics, Plant virus, Botany, Cultivar, Bioint Diagnostics, Food Safety & Phyt. Research, Pepino mosaic virus, biology, Inoculation, Host (biology), fungi, food and beverages, Test plants, biology.organism_classification, Potexvirus, PE&RC, Indicator plant, Food Safety & Phyt. Research, host plants, Host plants, Agronomy and Crop Science, Solanaceae
Abstract

Pepino mosaic virus (PepMV) has caused great concern in the greenhouse tomato industry after it was found causing a new disease in tomato in 1999. The objective of this paper is to investigate alternative hosts and compare important biological characteristics of the three PepMV strains occurring in Europe whentested under different environmental conditions. To this end we compared the infectivity and symptom Development of three, well characterized isolates belonging to three different PepMV strains, EU-tom, Ch2 and US1, by inoculating them on tomato, possible alternative host plants in the family Solanaceae and selected test plants. The inoculation experiments were done in 10 countriesfrom south to north in Europe. The importance of alternative hosts among the solanaceous crops and the usefulness of test plants in the biological characterization of PepMV isolates are discussed. Our data for the three strains tested at 10 different European locations with both international and local cultivars showed that eggplant is an alternative host of PepMV. Sweet pepper is not an important host of PepMV, but potato can be infected when the right isolate is matched with a specific cultivar. Nicotiana occidentalis 37B is a useful indicator plant for PepMV studies, since it reacts with a different symptomatology to each one of the PepMV strains.

Published
2015

19. Host range and symptomatology of Pepino mosaic virus strains occurring in Europe

Author

Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani, Ravnikar, M., Blystad, D.R., van der Vlugt, R., Alfaro Fernández, Ana Olvido, del Carmen Cordoba, M, Bese, G., Hristova, D., Pospieszny, H., Mehle, N., Ravnikar; Maja, Tomassoli, L, Varveri, C., Nielsen, S.L., Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani, Ravnikar, M., Blystad, D.R., van der Vlugt, R., Alfaro Fernández, Ana Olvido, del Carmen Cordoba, M, Bese, G., Hristova, D., Pospieszny, H., Mehle, N., Ravnikar; Maja, Tomassoli, L, Varveri, C., and Nielsen, S.L.

Abstract

Pepino mosaic virus (PepMV) has caused great concern in the greenhouse tomato industry after it was found causing a new disease in tomato in 1999. The objective of this paper is to investigate alternative hosts and compare important biological characteristics of the three PepMV strains occurring in Europe when tested under different environmental conditions. To this end we compared the infectivity and symptom development of three, well characterized isolates belonging to three different PepMV strains, EU-tom, Ch2 and US1, by inoculating them on tomato, possible alternative host plants in the family Solanaceae and selected test plants. The inoculation experiments were done in 10 countries from south to north in Europe. The importance of alternative hosts among the solanaceous crops and the usefulness of test plants in the biological characterization of PepMV isolates are discussed. Our data for the three strains tested at 10 different European locations with both international and local cultivars showed that eggplant is an alternative host of PepMV. Sweet pepper is not an important host of PepMV, but potato can be infected when the right isolate is matched with a specific cultivar. Nicotiana occidentalis 37B is a useful indicator plant for PepMV studies, since it reacts with a different symptomatology to each one of the PepMV strains.

Published
2015

20. New generation sequencing platforms for detection and characterization of viruses and viroids in citrus

Author

OLMOS, A., Bertolini, E., Varveri, C., Candresse, Thierry, Martinez, M.C., Pina, J.A., Cambra, M., Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

21. Analysis of siRNAs using a new generation sequencing platform for detection and characterization of viruses and viroids present in a citrus sample

Author

OLMOS, A., Varveri, C., Martinez, M.C., Bertolini, E., Candresse, Thierry, Cambra, M., Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research (IVIA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

25. Molecular characterization of greek Lettuce mosaic virus isolates

Author

Varveri, C., Peypelut, M., Le Gall, Olivier, Candresse, Thierry, Génomique, développement et pouvoir pathogène (GD2P), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)

Subjects
virus des vegetaux, lactuca sativa, [SDV]Life Sciences [q-bio], grèce, lmv, BIOLOGIE MOLECULAIRE, pays méditerranéen, maladie des plantes, mosaïque de la laitue, laitue, potyvirus, virus de la mosaïque de la laitue, europe, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2002

26. Variabilité moléculaire du LMV: identification de la souche LMV-Most

Author

Le Gall, Olivier, Krause-Sakate, Renate, Peypelut, Martine, Souche, Sylvie, LOT, Hervé, Pavan, M.A., Zerbini, F.M., Fakhfakh, Hatem, Varveri, C., Candresse, Thierry, ProdInra, Migration, Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV), Unité de Pathologie Végétale (PV), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], LMV-MOST, ComputingMilieux_MISCELLANEOUS, VIROLOGIE
Abstract

National audience

Published
2001

41. Characterization and detection of Cucumber green mottle mosaic virus in Greece.

Author

Varveri, C., Vassilakos, N., and Bem, F.

Abstract

The host range specificity of Greek isolates of Cucumber green mottle mosaic virus (CGMMV) was determined and the ability of the virus to retain its infectivity in naturally contaminated soil, after storage at 4°C for at least 10 months, was established. An immunocapture RT-PCR protocol was developed for the detection of the virus and proved to be 105 times more sensitive than DAS-ELISA and 102 times more sensitive than F(ab’)2-ELISA using F(ab’)2 fragments that were prepared from an antiserum, which was raised against a local virus isolate. In order to clarify dissimilarities that were revelaed between the coat protein (CP) genes of the Greek isolates after restriction mapping of their respective PCR products, the CP genes of three isolates were sequenced and found to be very similar but not identical to the CP gene of CGMMV-SH. [ABSTRACT FROM AUTHOR]

Published
2002
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42. Characterization and detection ofCucumber green mottle mosaic virusin Greece

Author

Varveri, C., Vassilakos, N., and Bem, F.

Abstract

The host range specificity of Greek isolates ofCucumber green mottle mosaic virus(CGMMV) was determined and the ability of the virus to retain its infectivity in naturally contaminated soil, after storage at 4°C for at least 10 months, was established. An immunocapture RT-PCR protocol was developed for the detection of the virus and proved to be 105times more sensitive than DAS-ELISA and 102times more sensitive than F(ab’)2-ELISA using F(ab’)2fragments that were prepared from an antiserum, which was raised against a local virus isolate. In order to clarify dissimilarities that were revelaed between the coat protein (CP) genes of the Greek isolates after restriction mapping of their respective PCR products, the CP genes of three isolates were sequenced and found to be very similar but not identical to the CP gene of CGMMV-SH.

Published
2002
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43. First report on Hop stunt viroid (HSVd) from some mediterranean countries

Author

Khalid Amari, Cañizares, M. C., Myrta, A., Sabanadzovic, S., Srhiri, M., Gavriel, I., Çaglayan, K., Varveri, C., Gatt, M., Di Terlizzi, B., and Pallás, V.

Subjects
lcsh:Botany, lcsh:QK1-989
Abstract

Hop stunt viroid (HSVd) has a very wide host range including most stone fruit trees. Among them, apricot is one of the most important host crops in the Mediterranean basin. In this study non-isotopic molecular hybridisation revealed, for the first time, the presence of HSVd on apricot in four Mediterranean countries (Cyprus, Greece, Morocco and Turkey). The results obtained by this technique were confirmed by northern-blot and RT-PCR analyses. The data presented in this work indicate a wider geographical distribution of this viroid than hitherto known and emphasise the need for this kind of study as part of the control effort.

44. Use of a 32P-labeled transcribed RNA probe for dot hybridization detection of plum pox virus

Author

Varveri, C., Thierry Candresse, Cugusi, M., Jean Dunez, Michel Ravelonandro, ProdInra, Migration, Unité mixte de recherche santé végétale, and Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)

Subjects
ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract

International audience

50. The Relationship Between Early Maladaptive Schemas and Cluster C Personality Disorder Traits: A Systematic Review and Meta-Analysis.

Author

Panagiotopoulos A, Despoti A, Varveri C, Wiegand MCA, and Lobbestael J

Subjects
Humans, Adaptation, Psychological, Surveys and Questionnaires, Personality Disorders, Personality
Abstract

Purpose of Review: We systematically reviewed and meta-analyzed the literature on the relationship between early maladaptive schemas (EMSs) and Cluster C personality disorders (PDs). Our aim was to clarify which of the 18 EMSs exhibit the strongest associations and are most frequently endorsed in clinical and non-clinical samples with Cluster C PDs and traits., Recent Findings: After initially screening 2622 records, 12 studies were selected with 5310 participants. Meta-analyses of the raw correlation coefficients for each EMS-Cluster C PD link (3-8 studies per meta-analysis) indicated that the 18 EMSs were significantly related to all three Cluster C PDs with r's ranging from .13 to .63. However, when considering endorsement rates among multiple regression studies that controlled for the EMSs intercorrelations and the effects of other PD traits and demographics, specific EMS constellations emerged for each Cluster C PD. Overall, the findings of the current paper suggest that Cluster C PDs might be conceptualized on the basis of a hybrid EMS model, in which all EMSs contribute to global personality dysfunction whereas specific EMS patterns reflect unique personality disorder style expressions. Longitudinal research with appropriate methodology is needed to draw more definite conclusions on the EMSs-Cluster C PDs relationships., (© 2023. The Author(s).)

Published
2023
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