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Contaminants are often found in aquatic environments, for instance, heavy metals, dyes, parasites, pesticides, hormones and pharmaceuticals. Therefore, large amounts of these contaminants reaches wastewater via industrial and domestic effluents, causing major concern to human health. Heterogeneous photocatalysis is a technique for removing these contaminants in order to achieve better efficiency in water treatment. Then, bacterial cellulose (BC) produced in an agitated culture can form spherical bodies composed of nanofibers with high specific surface area. Moreover, Titanium dioxide (TiO2) is a semiconductor containing high photocatalytic activity capacity. Thus, the main objective in this work was to produce spherical BC/TiO2 nanocomposites for contaminants removal from wastewater by photocatalysis process. The incorporation of TiO2 nanoparticles in the spherical BC matrix was performed by ex situ and in situ methods. In addition, Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FTIR) and Thermogravimetric Analysis (TGA) were used as tools of morphological, chemical and thermal characterizations of the nanocomposites. Besides, photocatalysis tests were performed in order to evaluate the removal efficiency of methylene blue from aqueous solutions. The results of these tests exhibited a percentage of methylene blue removal of 70.83 and 89.58 % after 35 minutes for spherical BC/TiO2 nanocomposites both, in situ and ex situ, respectively. Therefore, these results demonstrated that BC/TiO2 to be a low cost material with high capacity of contaminants removing and a great potential for industrial applications. [ABSTRACT FROM AUTHOR]