1. Oligandrin from Croton oligandrus (Euphorbiaceae) exhibits anti-breast cancer activity through immune-boosting mechanisms: In vitro and in vivo study
- Author
-
Stéphane Zingue, Yannick Stéphane Fotsing Fongang, Eric Roger Ossomba, Vanneck Tatsinda, Kevine Kamga Silihe, William Defo Mbou, Balotin Fogang, René Essomba, Jean Rodolphe Chouna, Dieudonné Njamen, and Lawrence Ayong
- Subjects
Oligandrin ,Croton oligandrus ,Breast cancer ,Immune system ,Cytokines ,Oxidative stress ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Aim: Recent developments in cancer research indicate that cancer is a manifestation of immune system dysfunction. Many natural anticancer agents developed recently possess immune-modulatory properties. In our ongoing pursuit of anticancer alternatives, we evaluated the immune-modulatory potential of oligandrin, an ent-pimarane type diterpenoid from Croton oligatrus. Methods: we assessed on Breast cancer patients’ peripheral blood mononuclear cells (PBMCs) were isolated to assess the effect of oligandrin (0.5, 1, 10, 100, 200 mg/mL) in vitro using the Ficoll-histopaque density centrifugation method. The parameters that were assessed included, PBMC viability and cytokine (IL-6, IL-12, IL-10, EGF, TNF-α, INF-γ) production. In vivo, we chemically induced breast cancer using DMBA (50 mg/kg BW) in Wistar rats, then treated them with oligandrin (1 mg/kg BW) or standards (tamoxifen 3.3 mg/kg; letrozole 1 mg/kg) for 20 weeks. The parameters that were evaluated included, tumor burden, volume, incidence, histopathology, antioxidant, and inflammatory status. Results: Oligandrin (1, 10, 100 and 200 μg/mL) significantly increased (p
- Published
- 2024
- Full Text
- View/download PDF