1. Optimization of a multiresidue and multiclass analysis method for anabolic agents and β2-agonists in bovine urine by GC-MS/MS
- Author
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Francisco Radler de Aquino Neto, Celeste Yara dos Santos Siqueira, Kelly Thaynan J. Santos, Vania Cristina da Silva Gonçalves, Virgínia Verônica de Lima, Monica Costa Padilha, and Nathália Oliveira Cavalcanti Zúniga
- Subjects
Analyte ,Chromatography ,Resolution (mass spectrometry) ,Anabolism ,Chemistry ,010401 analytical chemistry ,Extraction (chemistry) ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Tandem mass spectrometry ,01 natural sciences ,Anabolic Agents ,0104 chemical sciences ,Analytical Chemistry ,Gas chromatography ,Gas chromatography–mass spectrometry ,0210 nano-technology ,Spectroscopy - Abstract
A simple multiresidue and multiclass procedure is described for the simultaneous determination of anabolic agents and β2-agonists from 4 classes of prohibited substances of illegal use in food-producing animals, according to Council Directive 96/23/EC. The method involves analysis of hydrolysed urinary anabolic compounds using liquid–liquid extraction, with subsequent conversion to trimethylsilylether derivatives that are analysed by GC-MS/MS. The oven was set to an initial temperature of 140°C, was increased at a rate of 10°C/min to 300°C, and was held 3 min using an Ultra-1 capillary column. Optimization tests demonstrated good peak resolution and shape for all compounds. A pre-validation study was conducted to obtain an analytical test curve, which showed adequate results for all analytes. Cochran's test (C calculated
- Published
- 2017
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