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Your search keyword '"Vanhulle, V. P."' showing total 4 results
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4 results on '"Vanhulle, V. P."'

1. Cryopreservation of rat precision-cut liver slices by ultrarapid freezing: influence on phase I and II metabolism and on cell viability upon incubation for 24 hours

Author

UCL - MD/FARM - Ecole de pharmacie, UCL - MD/MINT - Département de médecine interne, UCL - (SLuc) Service de gastro-entérologie, UCL - (SLuc) Service de biologie hématologique, UCL - (SLuc) Centre de thérapie tissulaire et cellulaire, Vanhulle, V. P., Martiat, G. A., Verbeeck, Roger-K, Horsmans, Yves, Buc Calderon, Pedro, Eeckhoudt, S L, Taper, Henryk S, Delzenne, Nathalie M., UCL - MD/FARM - Ecole de pharmacie, UCL - MD/MINT - Département de médecine interne, UCL - (SLuc) Service de gastro-entérologie, UCL - (SLuc) Service de biologie hématologique, UCL - (SLuc) Centre de thérapie tissulaire et cellulaire, Vanhulle, V. P., Martiat, G. A., Verbeeck, Roger-K, Horsmans, Yves, Buc Calderon, Pedro, Eeckhoudt, S L, Taper, Henryk S, and Delzenne, Nathalie M.

Abstract

Several cryopreservation methods for precision-cut rat liver slices (PCLS) have been proposed, allowing a short-term (a few hours) maintainance of viability and functionality upon thawing. The aim of the present study was to test the metabolic capacity of PCLS cryopreserved by an ultrarapid method. The biotransformation of paracetamol to its glucuronide and sulfate conjugates and of midazolam to its hydroxylated metabolites was studied in thawed PCLS incubated for 24 hours at 37 degrees C in Williams' medium E. In addition, protein levels of the key enzymes involved in these metabolic reactions, i.e. UGT1A1, ST1A1, CYP2E1 and CYP3A2 were determinated. In addition, biological markers of cell function (ATP and glycogen levels) and toxicity (LDH leakage in the medium) were also measured. Compared to controls (non cryopreserved PCLS), CYP3A2 activity and content and CYP2E1 content were maintained at the same level all along the incubation, whereas paracetamol glucuronidation and sulfation dropped to 24 and 21% of the control value, respectively, immediately after thawing. Freezing-thawing conditions also modified cell functionality, leading to a lower intracellular ATP and glycogen content, and an increase in cell lysis, as shown by LDH released in the medium. The results of this study suggest that cryopreserved PCLS are able to maintain some phase I activities for 24 hours after thawing whereas some phase II metabolic capacities are not maintained.

Published
2001

4. Are Kupffer cells involved in the metabolic adaptation of the liver to dietary carbohydrates given after fasting?

Author

Neyrinck AM, De Wispelaere LD, Vanhulle VP, Taper HS, and Delzenne NM

Subjects
Adaptation, Physiological, Animals, Carbon, Dietary Carbohydrates administration & dosage, Enzyme Induction drug effects, Fasting, Fatty Acid Synthases biosynthesis, Fatty Acid Synthases genetics, Gadolinium pharmacology, Kupffer Cells drug effects, Liver enzymology, Male, Phagocytosis drug effects, RNA, Messenger analysis, Rats, Rats, Wistar, Triglycerides analysis, Triglycerides blood, Dietary Carbohydrates metabolism, Kupffer Cells physiology, Liver metabolism
Abstract

In rats, a high carbohydrate fat-free (HCFF) diet, given after fasting, induces both hepatic lipogenic and glycogenic enzymes. In the present study, we evaluated the involvement of Kupffer cells in the metabolic events occurring in the liver during the fasting-refeeding transition. Male Wistar rats were fasted for 48 h and received an intravenous injection of either NaCl 0.9% (Gd-) or 10 mg/kg GdCl(3) (Gd+), an inhibitor of Kupffer cells, then fed for 12 h with a HCFF diet. The comparison of colloidal carbon uptake was similar in rats fasted and in rats fasted and then refed a HCFF diet, thus indicating that refeeding does not affect per se Kupffer cell phagocytic activity. The inhibition of Kupffer cells by GdCl(3) did not affect fatty acid synthase (FAS) induction, as shown by the analysis of both FAS mRNA and activity; refeeding a HCFF diet increased the hepatic triglyceride and glycogen content to the same extent in Gd+ and Gd- rats. Our results do not support the involvement of Kupffer cells in the metabolic events occurring in the liver tissue by feeding a HCFF diet after fasting. However, the discussion supports the involvement of Kupffer cells in the modulation of the hepatic lipid metabolism by other nutrients than carbohydrates.

Published
2000
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