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Summary: Aerobic exercise training (AET) has emerged as a strategy to reduce cancer mortality, however, the mechanisms explaining AET on tumor development remain unclear. Tumors escape immune detection by generating immunosuppressive microenvironments and impaired T cell function, which is associated with T cell mitochondrial loss. AET improves mitochondrial content and function, thus we tested whether AET would modulate mitochondrial metabolism in tumor-infiltrating lymphocytes (TIL). Balb/c mice were subjected to a treadmill AET protocol prior to CT26 colon carcinoma cells injection and until tumor harvest. Tissue hypoxia, TIL infiltration and effector function, and mitochondrial content, morphology and function were evaluated. AET reduced tumor growth, improved survival, and decreased tumor hypoxia. An increased CD8+ TIL infiltration, IFN-γ and ATP production promoted by AET was correlated with reduced mitochondrial loss in these cells. Collectively, AET decreases tumor growth partially by increasing CD8+ TIL effector function through an improvement in their mitochondrial content and function.

This paper addresses subjective insecurity, namely perceptions of (in)security and criminal variables on campus among Portuguese higher education students. Additionally, predictors of perceptions of (in)security and gender differences were also examined. The participants were 775 students and data were collected through the “Diagnosis of Local Security Questionnaire”. Robbery, physical assault, theft, and sexual offenses were the most feared crimes. Additionally, robbery, theft, and public property damage were perceived as the most common on campus. Alcohol/drug consumption and juvenile conflicts/delinquency were the main reasons justifying criminal occurrences. Sociodemographic variables such as gender, age, education, and years of campus attendance, as well as criminal variables (e.g., perceived trend of crime, criminal occurrences, and crime promoters) predicted perceptions of (in)security. Females reported more fear than males of robbery, sexual offenses, physical aggression, and domestic violence. Therefore, preventive measures, including in the social domain and physical spaces, are mandatory to reduce violence on campus.

Abstract Background Most cancer patients, under active treatment or not, are sedentary, despite increasing scientific and clinical understanding of the benefits of exercise and physical activity, such as improving quality of life, limiting disease symptoms, decreasing cancer recurrence, and increasing overall survival. Studies have shown that both supervised exercise and unsupervised physical activity programs have low adherence and limited long-term benefits among cancer survivors. Therefore, interventions focused on increasing physical activity levels have clinical and psychological relevance. The present study will examine the feasibility and efficacy of an intervention that combines supervised group exercise with active lifestyle recommendations, analyzing its clinical, psychological, physiological, functional, and immunological effects in breast cancer survivors. Methods Women aged 35–75 years who have completed chemotherapy, radiotherapy, and surgery for breast cancer will be recruited from the Cancer Institute of the State of Sao Paulo (ICESP) and take part in a 16-week, parallel-group, randomized, and controlled trial. They will receive a booklet with recommendations for achieving a physically active lifestyle by increasing overall daily movement and undertaking at least 150 min/week of structured exercise. Then, they will be randomized into two groups: the supervised group will take part in two canoeing group exercise sessions every week, and the unsupervised group will increase their overall physical activity level by any means, such as active commuting, daily activities, or home-based exercise. Primary outcome includes aerobic capacity. Secondary outcomes are physical activity, physical functioning, self-reported quality of life, fatigue, presence of lymphedema, body composition, immune function, adherence to physical activity guidelines, and perceptions of self-image. Discussion Results should contribute to advance knowledge on the impact of a supervised group exercise intervention to improve aspects related to health, physical functioning, and quality of life in female breast cancer survivors. Trial registration Brazilian Registry of Clinical Trials Number: RBR-3fw9xf. Retrospectively Registered on 27 December 2018. Items from the World Health Organization Trial Registration Data Set can be accessed on http://www.ensaiosclinicos.gov.br/rg/RBR-3fw9xf/ .

This study aims to provide answers to the following questions: Do you feel safe at Historic Centre of Porto (HCP)? Do you think that crime is increasing? What are the most and less frequent crimes? And the most feared crimes? What conditions promote criminal occurrences? What incivilities occur at HCP? What measures can be taken to increase security? What variables are related to the perception of insecurity? Through the application of the Diagnosis of Local Security Questionnaire, 554 participants that attended HCP were assessed (58.5% women, mean age = 43.82). One in five participants reported feelings of insecurity due to the presence of crime/danger. Robbery, theft, and drugs traffic were perceived as the most common crimes; participants were especially fearful about robbery and theft. Nationality, education, criminal variables, adequacy and satisfaction with policing, seek for formal support, and years living/studying/working at HCP were variables related to perception of (in)security.

Heme is an iron-protoporphyrin complex with an essential physiologic function for all cells, especially for those in which heme is a key prosthetic group of proteins such as hemoglobin, myoglobin, and cytochromes of the mitochondria. However, it is also known that heme can participate in pro-oxidant and pro-inflammatory responses, leading to cytotoxicity in various tissues and organs such as the kidney, brain, heart, liver, and in immune cells. Indeed, heme, released as a result of tissue damage, can stimulate local and remote inflammatory reactions. These can initiate innate immune responses that, if left uncontrolled, can compound primary injuries and promote organ failure. In contrast, a cadre of heme receptors are arrayed on the plasma membrane that is designed either for heme import into the cell, or for the purpose of activating specific signaling pathways. Thus, free heme can serve either as a deleterious molecule, or one that can traffic and initiate highly specific cellular responses that are teleologically important for survival. Herein, we review heme metabolism and signaling pathways, including heme synthesis, degradation, and scavenging. We will focus on trauma and inflammatory diseases, including traumatic brain injury, trauma-related sepsis, cancer, and cardiovascular diseases where current work suggests that heme may be most important.

This article describes a curated dataset entitled “Looking at Crime: Communities and Physical Spaces”, which comprises data from different sources, namely Diagnosis of Local Security (DLS), Diagnosis of School Environment (DSE) and observation of physical spaces. The main topic covered was crime and related variables at the Historic Centre of Porto (HCP), a well-known urban area located in the North of Portugal. It is currently attended by inhabitants, workers, students and tourists. This dataset includes i) data from two different self-reports: i.1) demographics, perception of (in)security, victimization, social control and community cohesion obtained through an inquiry applied to the adult community; and i.2) data from school climate and students behavioural problems, which may be seen as risk factors for juvenile delinquency, collected through a web-survey applied to school personnel; and ii) data from observation of physical spaces, attending to the Crime Prevention through Environmental Design (CPTED) principles. The dataset allows descriptive and inferential statistical analyses, being useful for students, academics, stakeholders, police forces and policy-makers, to better understand crime and its related variables, forecast criminal incidents, and further develop associated preventive and intervention programmes.

The lack of organisation in urban spaces plays a decisive role in the level of integration, communication and social bonds of the residents, impacting the citizens’ feelings of trust and security. Different personal variables and contextual characteristics have been associated with the fear of crime (FOC). The main objective of this study is to analyse how individual and social/environmental variables, and incivilities, predict crime against people and property, crime that has either happened or is feared to happen. Five hundred and fifty-four residents (M = 43.82; SD = 18.38) in the Historic Centre of Porto (HCP), Portugal, answered 61 items of the Diagnosis of Local Security (DLS) Questionnaire. The results of this study show that in the most frequent crime category, 72% of occurrences represent crime against property. In the feared crime category, there is a preponderance of crime against people (61%). Age of the respondents predicted the most frequent and feared crime, while sex predicted the most feared crime only. Social/environmental variables, as well as incivilities, also predict the frequent and feared crime in two typologies, i.e., crime against people and crime against property. Practical implications to reduce FOC and areas for further investigation are discussed.

Most studies about life experiences and their long-term impact have relied on retrospective assessments and cross-sectional designs. However, there are concerns about the inconsistency of reports, which have been addressed in a limited scope. This study aimed to compare differences on inconsistency between positive and negative experiences to describe potential patterns and to identify sociodemographic, experiences-related and design-related predictors of inconsistency. Adults from community (N = 171) reported their life experiences twice, through self-report or interview. An overall trend of overreporting was found for positive and negative experiences. Additionally, inconsistency on positive experiences was higher than on negative although both variables were correlated. Regarding predictors of inconsistency, the model explained greater variance for negative experiences than for the positive ones. Nevertheless, most variables did not predict inconsistency, with few exceptions: impact for positive experiences, total of experiences for negative experiences, and time interval was marginally significant for both. Available data comparing inconsistency on positive and negative experiences as well as associated predictors are incipient. Overall, it seems that we know more about variables not involved in inconsistency than those that matter. Due to its relevancy, this issue should be further examined.

This quantitative, correlational, and transversal study was performed with a sample of 482 participants from an urban community at the Historic Centre of Porto (HCP). Participants answered to an enquiry designed to collect information about (dis)satisfaction with the police and its performance, with sociodemographic, victimisation, criminal, environmental, social control, and community variables as potential predictors. Findings revealed that the community was mainly satisfied with the police in its efforts to guarantee security, and there was no relationship between those variables, and sociodemographic and some community variables (e.g., years at the HCP, willingness to collaborate in security measures, and strength of attachment to HCP). On the other hand, there were relationships of (dis)satisfaction with the police and being the victim of crime, and some criminal and environmental variables (e.g., perception of increased criminality, conditions promoting crime, and incivilities). Regression analyses found that the perception of increased criminality and the need to adopt improvement measures were significant predictors of dissatisfaction with the police. This study promotes further discussion on factors that can be improved to increase satisfaction with the police and the connection of community–institutions to promote community security.

Criminology theories imply that time is a relevant variable, especially for the prevention and intervention of criminal occurrences. Thus, the study of criminal temporal patterns has been described as being of great relevance. The present study focuses on describing and exploring the influence of temporal and seasonal variables on the occurrence of different types of theft in the Historic Centre of Porto through the analysis of official records of the Public Security Police. Significant differences were found regarding the time of day and season of occurrence, even though it is not observed for all the types of theft analysed. Overall, theft was more prevalent at night and less frequent during winter, which is congruent with previous literature and the routine activity theory. Being the first case study in Porto city, Portugal, this research may be of extreme importance for both designing prevention and intervention policies in the area, and for inspiring future research on a criminal time analysis.

Este trabajo es fruto de reflexiones y del diálogo abordado en la disciplina de Epistemologías del Sur del Programa de Postgrado en Educación Contemporánea (PPGduc), en la Universidad Federal de Pernambuco-Centro Académico del Agreste (UFPE-CAA). En este trabajo abordamos como cuestión problema: ¿cuáles son los principales sentidos de las historicidades de las mujeres negras presentes en el libro didáctico (LD) del Campo? El objetivo general fue: comprender los principales sentidos atribuidos a las historicidades de las mujeres negras. Los objetivos específicos son: a) identificar y caracterizar las historias presentes en los LD sobre las mujeres negras; b) describir los sentidos atribuidos a las historicidades de las mujeres negras en los LD; y c) analizar en los LD los elementos presentes en las historicidades que contribuyen o no a la afirmación étnica de mujeres negras. En los procedimientos metodológicos utilizamos la investigación cualitativa y documental pautada en el análisis del contenido. No encontramos de forma directa historicidades de mujeres negras que contribuyeron a la historia, que pudiera romper con el silenciamiento puesto sobre la presencia de las mujeres en espacio como la política, la cultura, las artes, entre otros.

The molecular mechanisms underlying skeletal muscle mitochondrial adaptations induced by aerobic exercise (AE) are not fully understood. We have previously shown that AE induces mitochondrial adaptations in cardiac muscle, mediated by sympathetic stimulation. Since direct sympathetic innervation of neuromuscular junctions influences skeletal muscle homeostasis, we tested the hypothesis that β2-adrenergic receptor (β2-AR)-mediated sympathetic activation induces mitochondrial adaptations to AE in skeletal muscle. Male FVB mice were subjected to a single bout of AE on a treadmill (80% Vmax, 60 min) under β2-AR blockade with ICI 118,551 (ICI) or vehicle, and parameters of mitochondrial function and morphology/dynamics were evaluated. An acute bout of AE significantly increased maximal mitochondrial respiration in tibialis anterior (TA) isolated fiber bundles, which was prevented by β2-AR blockade. This increased mitochondrial function after AE was accompanied by a change in mitochondrial morphology towards fusion, associated with increased Mfn1 protein expression and activity. β2-AR blockade fully prevented the increase in Mfn1 activity and reduced mitochondrial elongation. To determine the mechanisms involved in mitochondrial modulation by β2-AR activation in skeletal muscle during AE, we used C2C12 myotubes, treated with the non-selective β-AR agonist isoproterenol (ISO) in the presence of the specific β2-AR antagonist ICI or during protein kinase A (PKA) and Gαi protein blockade. Our in vitro data show that β-AR activation significantly increases mitochondrial respiration in myotubes, and this response was dependent on β2-AR activation through a Gαs-PKA signaling cascade. In conclusion, we provide evidence for AE-induced β2-AR activation as a major mechanism leading to alterations in mitochondria function and morphology/dynamics. β2-AR signaling is thus a key-signaling pathway that contributes to skeletal muscle plasticity in response to exercise.

The aim of this study is to evaluate the effect of the hyperproteic diet consumption on bone tissue. Methods: The study was conducted during sixty days. Twenty eight Wistar albinus rats, adults, originated from Laboratory of Experimental Nutrition were divided in four groups: (n = 7); Control 1 (C1), Control 2 (C2), Hyperproteic 1 (HP1) e Hyperproteic 2 (HP2). The C2 and HP2 groups were submitted to 30% of food restriction. The hyperproteic diet was based on the Atkins diet and prepared to simulate the protein diet. At the end of the study the animals were anesthetized to performer bone densitometry analyses by DEXA and blood and tissue collection. Serum and bone minerals analyses were conducted by colorimetric methods in automated equipment. Results: The total bone mineral density (BMD) of the pelvis and the spine of the food restriction groups (HP2 e C2) were lower (p < 0.05) than C1 e HP1 groups. While the femur BMD of the HP2 was lower (p < 0.05) related to others groups. It had been observed reduction (p < 0.05) in the medium point of the width of femur diaphysis and in bone calcium level in the hyperproteic groups (HP1 e HP2). It was observed similar effect on the osteocalcin level, that presented lower (p < 0.05) in the hyperproteic groups. The insulin level was lower only in HP2 and serum calcium of the HP1 and HP2 groups was lower than C1. Conclusion: The protein diet promotes significant bone change on femur and in the hormones levels related to bone synthesis and maintenance of this tissue.

Nanoparticles were produced by solvent emulsification evaporation method with the following characteristics: nanometric size (238 ± 3 nm), narrow polydispersity index (0.11), negative zeta potential (-15.1 mV), good yield of the process (73 ± 1.5%), excellent encapsulation efficiency (81.3 ± 4.2%) and spherical shape. X-rays diffraction demonstrated the loss of drug crystallinity after encapsulation; however, the profile of the diffractograms of the poly-ε-caprolactone (PCL) nanoparticles was kept. Differential scanning calorimetry thermograms, correspondingly, exhibited the loss of drug melting peak and the increasing of the melting point of the PCL nanoparticles, evidencing an interaction drug-polymer. Naproxen release was low and sustained obeying the Higuchi´s kinetic. The results show that nanoparticles are promising sustained release system to the naproxen.

DNA methylation has been referred as an important player in plant genomic responses to environmental stresses but correlations between the methylome plasticity and specific traits of interest are still far from being understood. In this study, we inspected global DNA methylation levels in salt tolerant and sensitive rice varieties upon salt stress imposition. Global DNA methylation was quantified using the 5-methylcytosine (5mC) antibody and an ELISA-based technique, which is an affordable and quite pioneer assay in plants, and in situ imaging of methylation sites in interphase nuclei of tissue sections. Variations of global DNA methylation levels in response to salt stress were tissue- and genotype-dependent. We show a connection between a higher ability of DNA methylation adjustment levels and salt stress tolerance. The salt-tolerant rice variety Pokkali was remarkable in its ability to quickly relax DNA methylation in response to salt stress. In spite of the same tendency for reduction of global methylation under salinity, in the salt-sensitive rice variety IR29 such reduction was not statistically supported. In 'Pokkali', the salt stress-induced demethylation may be linked to active demethylation due to increased expression of DNA demethylases under salt stress. In 'IR29', the induction of both DNA demethylases and methyltransferases may explain the lower plasticity of DNA methylation. We further show that mutations for epigenetic regulators affected specific phenotypic parameters related to salinity tolerance, such as the root length and biomass. This work emphasizes the role of differential methylome flexibility between salt tolerant and salt sensitive rice varieties as an important player in salt stress tolerance, reinforcing the need to better understand the connection between epigenetic networks and plant responses to environmental stresses.

Skeletal muscle protein turnover is modulated by intracellular signaling pathways involved in protein synthesis, degradation, and inflammation. The proinflammatory status of muscle cells, observed in pathological conditions such as cancer, aging, and sepsis, can directly modulate protein translation initiation and muscle proteolysis, contributing to negative protein turnover. In this context, branched-chain amino acids (BCAAs), especially leucine, have been described as a strong nutritional stimulus able to enhance protein translation initiation and attenuate proteolysis. Furthermore, under inflammatory conditions, BCAA can be transaminated to glutamate in order to increase glutamine synthesis, which is a substrate highly consumed by inflammatory cells such as macrophages. The present paper describes the role of inflammation on muscle remodeling and the possible metabolic and cellular effects of BCAA supplementation in the modulation of inflammatory status of skeletal muscle and the consequences on protein synthesis and degradation.

Este trabalho constitui um desdobramento da pesquisa "Avaliação psicossomática do risco de adoecimento em mulheres com história familiar de câncer de mama", realizada no Centro de Psicologia Aplicada da Universidade Federal de Juiz de Fora, Minas Gerais, desde 1999. Propõe, neste sentido, uma articulação entre os níveis individual e familiar da avaliação psicossomática do risco de adoecimento nesse tipo de enfermidade. Destaca, nessa direção, a função materna como operador de análise, buscando dar conta de ambos os níveis de avaliação: no nível individual, remetendo à qualidade da internalização da função de continente provida pela mãe; e, no nível familiar, buscando capturar, pelos mecanismos de interfantasmatização, a eficácia do grupo familiar como representante da função materna continente. Baseia-se, para tanto, na Psicossomática Psicanalítica, sobretudo nos estudos da Escola de Psicossomática de Paris sobre o funcionamento mental nas somatizações, e em psicanalistas estudiosos da família, concebida como "corpo materno" e lugar de ancoragem das excitações, conflitos e fantasmas circulantes entre os membros do grupo. A partir do estudo de um dos casos investigados na pesquisa, lança-se mão da Análise de Discurso, tanto nas três entrevistas individuais quanto nas três entrevistas de família. O caso tomado demonstrou ser adequado ao propósito do estudo em questão.

Prosopis juliflora (Fabaceae) is a small perennial tree used as human food and livestock feed for thousands of years in arid and semi-arid regions worldwide. This study was performed to investigate the in vivo antimalarial activity of alkaloid-enriched extracts and pure alkaloidal constituents of Prosopis juliflora in a murine model. Alkaloid-enriched extracts from leaves (FACB) and pods (BCE) of P. juliflora, as well as FACB pure constituents (as formate salts), were obtained and assayed against Plasmodium berghei NK65 infection in mice via oral administration. FACB and BCE exhibited a remarkable in vivo antimalarial activity with little parasitemia inhibition at the 2 mg/kg dose. Additionally, julifloridine was weakly active, but juliprosopine caused a parasitemia inhibition at the 2 mg/kg dose similar to that observed for chloroquine at 50 mg/kg. This study reports for the first time the in vivo antimalarial activity of julifloridine, juliprosopine and alkaloid-enriched extracts from P. juliflora (Fabaceae).