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41 results on '"Vandewoestyne, Mado"'

1. Cell Nuclei Detection Using Globally Optimal Active Contours with Shape Prior

Author

De Vylder, Jonas, Aelterman, Jan, Vandewoestyne, Mado, Lepez, Trees, Deforce, Dieter, Philips, Wilfried, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Bebis, George, editor, Boyle, Richard, editor, Parvin, Bahram, editor, Koracin, Darko, editor, Fowlkes, Charless, editor, Wang, Sen, editor, Choi, Min-Hyung, editor, Mantler, Stephan, editor, Schulze, Jürgen, editor, Acevedo, Daniel, editor, Mueller, Klaus, editor, and Papka, Michael, editor

Published
2012
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25. Impact of allelic dropout on evidential value of forensic DNA profiles

Author

Van Nieuwerburgh, Filip, Goetghebeur, Els, Vandewoestyne, Mado, and Deforce, Dieter

Subjects
mixtures
Abstract

Motivation: Two methods are commonly used to report on evidence carried by forensic DNA profiles: the 'Random Man Not Excluded' (RMNE) approach and the likelihood ratio (LR) approach. It is often claimed a major advantage of the LR method that dropout can be assessed probabilistically. Results: In this article, a new RMNE measure is proposed that likewise accounts for allelic dropout in an observed forensic DNA pro. le. We discuss the necessary calculations, underline their simplicity and provide a tool for performing the calculations.

Published
2009

31. The novel ‘CandidatusAmphibiichlamydia ranarum’ is highly prevalent in invasive exotic bullfrogs (Lithobates catesbeianus)

Author

Martel, An, primary, Adriaensen, Connie, additional, Sharifian‐Fard, Mojdeh, additional, Vandewoestyne, Mado, additional, Deforce, Dieter, additional, Favoreel, Herman, additional, Bergen, Karolien, additional, Spitzen‐van der Sluijs, Annemarieke, additional, Devisscher, Sander, additional, Adriaens, Tim, additional, Louette, Gerald, additional, Baert, Kristof, additional, Hyatt, Alex, additional, Crameri, Sandra, additional, Haesebrouck, Freddy, additional, and Pasmans, Frank, additional

Published
2012
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35. Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization

Author

Vandewoestyne, Mado, primary, Kumps, Candy, additional, Swerts, Katrien, additional, Menten, Björn, additional, Lammens, Tim, additional, Philippé, Jan, additional, De Preter, Katleen, additional, Laureys, Geneviève, additional, Van Roy, Nadine, additional, Speleman, Frank, additional, and Deforce, Dieter, additional

Published
2011
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41. Predominant mucosal expression of 5-HT₄(+h) receptor splice variants in pig stomach and colon.

Author

Priem EK, De Maeyer JH, Vandewoestyne M, Deforce D, and Lefebvre RA

Subjects
Amino Acid Sequence, Animals, Base Sequence, Colon pathology, Epithelium metabolism, Epithelium pathology, Gastric Mucosa pathology, Intestinal Mucosa pathology, Male, Models, Animal, Molecular Sequence Data, RNA metabolism, Stomach pathology, Swine, Colon metabolism, Gastric Mucosa metabolism, Intestinal Mucosa metabolism, Protein Isoforms metabolism, Receptors, Serotonin, 5-HT4 metabolism
Abstract

Aim: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser microdissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues., Methods: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections and in microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohistochemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR., Results: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 receptor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P < 0.01) compared to 5-HT4(-h) receptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH., Conclusion: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mucosal effects of 5-HT.

Published
2013
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