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We compared the genetic diversity and structure of wild versus captive-propagated at-risk species of freshwater mussels in Canada, Lampsilis fasciola (wavy-rayed lampmussel; WRL) and Ptychobranchus fasciolaris (kidneyshell; KS), using microsatellite-generated genotypes. No significant differences were detected using Kruskal-Wallis tests that compared the genetic diversity metrics of wild and captive-propagated WRL (from 12 mothers) and KS (from seven mothers). After effective population size ([N.sub.e]) was estimated, considerable overlap was found in the estimated [N.sub.e] between wild and captive populations of both species. Pairwise [F.sub.ST] and [D.sub.est] values among wild WRL, hatchery-raised WRL juveniles, and across different time periods (2008-2018) were generally low ([F.sub.ST] = 0.007-0.043, [D.sub.est] = 0.005-0.282). Pairwise [F.sub.ST] and [D.sub.est] for KS were not significant and low (0.012, 0.017). When comparing captive-reared offspring with the wild population, STRUCTURE analysis revealed strong support for a single genetic population. With at least seven females contributing to brood stock, genetic diversity was maintained. Further research is needed to assess the male contribution to each glochidial brood as well as the variability of genetic diversity in individual glochidial broods. Nous avons compare la diversite et la structure genetiques de deux mulettes en peril au Canada, la lampsile fasciolee (Lampsilis fasciola, LF) et le ptychobranche reniforme (Ptychobranchusfasciolaris, PF), sauvages et issues de la propagation en captivite, en utilisant des genotypes generes a partir de microsatellites. Aucune difference significative n'est relevee entre les populations sauvages et propagees en captivite de LF (issues de 12 meres) ou de PF (issues de sept meres) a la lumiere de differents parametres de diversite genetique en utilisant des tests de Kruskal-Wallis. La taille effective de la population ([N.sub.e]) a ete estimee, et un chevauchement considerable est releve entre les [N.sub.e] estimees des populations sauvages et propagees en captivite des deux especes. Les valeurs de [F.sub.ST] et [D.sub.est] par paire de LF sauvages, de LF juveniles elevees en ecloseries et pour differentes periodes (de 2008 a 2018) sont generalement faibles ([F.sub.ST] = 0,007-0,043, [D.sub.est] = 0,005-0,282). Les valeurs de [F.sub.ST] et [D.sub.est] par paire de PF sont faibles (0,012, 0,017) et non significatives. Pour la comparaison de progeniture elevee en captivite a la population sauvage, les resultats de l'analyse avec le logiciel STRUCTURE appuient fortement l'interpretation d'une seule population genetique. Si au moins sept femelles contribuent au stock de geniteurs, la diversite genetique est maintenue. D'autres travaux de recherche sont necessaires pour evaluer la contribution des males a chaque emission de glochidies et la variabilite de la diversite genetique au sein de ces emissions. [Traduit par la Redaction], Introduction Freshwater mussels (Bivalvia: Unionida, unionids) are one of the most imperilled taxa on the planet, with 70% of unionid species in North America at risk of extinction (Williams et [...]

We compared the genetic diversity and structure of wild versus captive-propagated at-risk species of freshwater mussels in Canada, Lampsilis fasciola (wavy-rayed lampmussel; WRL) and Ptychobranchus fasciolaris (kidneyshell; KS), using microsatellite-generated genotypes. No significant differences were detected using Kruskal–Wallis tests that compared the genetic diversity metrics of wild and captive-propagated WRL (from 12 mothers) and KS (from seven mothers). After effective population size (Ne) was estimated, considerable overlap was found in the estimated Ne between wild and captive populations of both species. Pairwise FST and Dest values among wild WRL, hatchery-raised WRL juveniles, and across different time periods (2008–2018) were generally low (FST = 0.007–0.043, Dest = 0.005–0.282). Pairwise FST and Dest for KS were not significant and low (0.012, 0.017). When comparing captive-reared offspring with the wild population, STRUCTURE analysis revealed strong support for a single genetic population. With at least seven females contributing to brood stock, genetic diversity was maintained. Further research is needed to assess the male contribution to each glochidial brood as well as the variability of genetic diversity in individual glochidial broods. [ABSTRACT FROM AUTHOR]

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