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5. Towards the determination of the dissolved CO2 concentration in aqueous solutions under extreme climatic stress conditions.

Author

Roufou, S., Griffin, S., de Oliveira Mallia, J., Katsini, L., Polańska, M., Van Impe, J. F. M., Gatt, R., and Valdramidis, V. P.

Subjects
ATMOSPHERIC carbon dioxide, CLIMATE extremes, AQUEOUS solutions, CARBON dioxide, OCEAN acidification, BICARBONATE ions
Abstract

The global carbon dioxide in the atmosphere is predicted to rise due to fossil fuel emissions during the next century. Carbon dioxide is soluble in water and partially dissociates into bicarbonate and carbonate, releasing protons that decrease the pH. This change in pH is expected to play a critical role in some earth systems, such as the hydrosphere, where ocean acidification affects the survival of marine organisms. This study used aqueous solutions consisting of water with sodium bicarbonate, Miller's lysogeny broth, Roswell Park Memorial Institute 1640 medium, and Dulbecco's Modified Eagle Medium to develop a colourimetric method for the quantification of dissolved carbon dioxide. Various environmental testing conditions were studied using a fluorescent microplate spectrophotometer. The temperature varied between 27 and 42 °C, and the carbon dioxide levels ranged from 0.20 to 10% v/v in the air. The data showed that the amount of dissolved carbon dioxide decreased by 50% in broth solutions, while the reduction in water samples was approximately 18% when the temperature rose from 27 to 42 °C. Furthermore, the composition of the solutions impacted the amount of gas dissolved. The highest amount of dissolved carbon dioxide was observed in the water sample with the lowest salinity, which reached 20,000 ppm CO2 at 27 °C. The lowest amount of dissolved carbon dioxide concentrations was obtained in Miller's lysogeny broth with the highest salinity level, which reached 1200 ppm under the same conditions. The results obtained can be considered a robust estimation method to calculate the amount of dissolved carbon dioxide under different conditions. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Thermophysical properties of the fe48cr15mo14c15b6y2 alloy in liquid state

Author

Kozak, D. S., Tsepelev, V. S., Konaskov, V. V., Vyukhin, V. V., Zadorozhnyy, V. Y., Bazlov, A. I., Kvaratskheliya, A. R., Tsarkov, A. A., Van, Impe, J. F. M., Kozak, D. S., Tsepelev, V. S., Konaskov, V. V., Vyukhin, V. V., Zadorozhnyy, V. Y., Bazlov, A. I., Kvaratskheliya, A. R., Tsarkov, A. A., and Van, Impe, J. F. M.

Abstract

In this work, the physical properties of Fe48Cr15Mo14C15B6Y2 alloy in liquid state at high temperature are studied. It was observed that the basic physical characteristics of the alloy, such as viscosity, electrical resistivity, and density, decrease with an increase of the temperature to 1700◦C. An abnormal increasing rate of viscosity for Fe48Cr15Mo14C15B6Y2 alloy in the temperature range from 1360 to 1550◦C was noted. The measurement of the electrical resistivity and density did not reveal any anomalies in the same temperature range. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Published
2021

27. Design of a Low‐Power Radio Frequency Unit and Its Application for Bacterial Inactivation under Laboratory Conditions

Author

Kozak, D. S., Tonti, M., Cuba, P., Espitia, J., Tsepelev, V. S., Verheyen, D., Akkermans, S., Van Impe, J. F. M., Kozak, D. S., Tonti, M., Cuba, P., Espitia, J., Tsepelev, V. S., Verheyen, D., Akkermans, S., and Van Impe, J. F. M.

Abstract

A lab‐scale low‐power free‐running radio frequency (RF) oscillator operating at a frequency of 27.12 ± 0.50 MHz was developed to be suitable for fundamental microbiological research topics. Calibration and validation were conducted for two common foodborne pathogens in relevant microbiological growth media, i.e., Salmonella Typhimurium and Listeria monocytogenes in Tryptic Soy Broth and Brain–Heart Infusion broth, respectively. The evolution of temperature, frequency, and power consumption was monitored during treatments, both with and without bacterial cells. The setup operated within the predefined frequency range, reaching temperatures of 71–76 °C after 15 min. The average power consumption ranged between 12 and 14 W. The presence of bacteria did not significantly influence the operational parameters. The inactivation potential of the RF setup was validated, demonstrating the absence of viable cells after 8 and 10 min of treatment, for S. Typhimurium and L. monocytogenes, respectively. In future studies, the setup can be used to conduct fundamental microbiological studies on RF inactivation. The setup can provide added value to the scientific field, since (i) no consensus has been reached on the inactivation mechanisms of RF inactivation of pathogens in foods and (ii) most commercial RF setups are unsuitable to adopt for fundamental studies. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Published
2021

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