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Background Temporomandibular disorders (TMD) are a group of painful and debilitating disorders, involving the masticatory muscles and/or the temporomandibular joint (TMJ). Chronic TMD pain can be associated with genetic changes in the key muscle development genes. Objective To evaluate the association between polymorphisms in the PAX7 (paired box 7) gene and masticatory myalgia in patients with temporomandibular disorders (TMD). Materials and methods This is a case-control study. Patients with TMD were divided into two groups: (a) presence of muscular TMD (n = 122) and (b) absence of muscular TMD (n = 49). Genomic DNA was obtained from saliva samples from all participants to allow for genotyping single nucleotide polymorphisms in PAX7 (rs766325 and rs6659735). Over-representation of alleles was tested using chi-square or Fisher’s exact tests. Values of p Results Individuals without muscular TMD were less likely to have the PAX7 rs6659735 GG genotype (p = 0.03). No associations were found for PAX7 rs766325. Conclusions Alterations in PAX7 may influence muscular pathophysiology and individuals with TMD and the rs6659735 homozygous genotype (GG) are seemingly associated with muscular involvement of the disorder. No associations were found in the region rs766325.

Atualmente, no Brasil e no mundo, o tabaco vem sendo consumido por milhões de pessoas, tornando-se um grave problema de saúde pública, apresentando-se como uma das principais causa de morte evitáveis; pois acabam sendo vítimas de diversos agravos e de doenças causadas pelo consumo desta substância. Apesar de todo o conhecimento científico acumulado sobre os riscos do tabaco, o consumo continua alarmante e preocupante, pelo fato desta prática ocasionar para os usuários e para aqueles que o rodeiam vários tipos doenças, como também piorar a qualidade de meio ambiente, justifica-se a necessidade de uma intervenção eficaz para o usuário que deseja abster-se deste vício. A medida mais eficaz e economicamente viável para minimizar o impacto negativo do fumo na saúde dos cidadãos é a promoção e a prevenção em saúde, desenvolvidas na Atenção Primária à Saúde iniciando de preferencia na infância para tornar os adolescentes livres do consumo. Este trabalho tem com objetivo evidenciar a relação saúde/doenças causadas pelo consumo do tabaco e potencializar as discussões sobre as ações de educação em saúde sobre o tabagismo. Evidenciando assim, a importância do tratamento, conhecimento e conscientização como ferramentas de prevenção do tabagismo, consequentemente alcançar a diminuição do índice de fumantes, e motivar uma mudança no estilo de vida, na atitude e no comportamento, diminuindo as situações de riscos que estão expostos.

O serviço de saúde em emergências tem sido objeto de estudo no cenário nacional e internacional. A superlotação dos serviços de urgência representa uma manifestação local de um problema que afeta a todo o sistema de saúde, o qual ocorre tanto no setor público quanto no privado. Sendo assim, o presente trabalho visa compreender o funcionamento dos serviços de urgência e emergência, mostrar o cenário atual dos serviços citados, analisar as causas e consequências da superlotação dos serviços de urgência hospitalar, evidenciar algumas estratégias que supram as necessidades deste sistema, destacando o programa de saúde de Investimento de Qualificação do Sistema Único de Saúde (QualiSUS), aumento de Unidades de Pronto Socorro (UPAS) tendo como objetivo geral discorrer sobre motivos e consequências para superlotação nos serviços hospitalares de urgência.Este estudo foi desenvolvido por meio de uma revisão bibliográfica com base em artigos científicos e publicações do Ministério da Saúde (MS) que abordassem o tema sobre superlotação e características dos usuários que procuram o serviço de urgência e emergência. Neste sentido, constata que a principal forma de resolução para problemas como esse é a implementação de políticas públicas.

The aim of this study was to evaluate the possible association between polymorphisms in the catechol- O -methyltransferase ( COMT ) and β2-adrenergic receptor ( ADRB2 ) genes and muscular temporomandibular disorders (TMD). This was a case–control study. Individuals were evaluated using the Research Diagnostic Criteria for Temporomandibular Disorders and were divided into three groups: unaffected (no TMD) ( n = 154); exclusively muscular TMD ( n = 49); exclusively articular TMD ( n = 49). Genomic DNA was obtained from saliva samples, and single nucleotide polymorphisms in the COMT (rs165774, rs6269, rs9332377) and ADRB2 (rs2053044, rs1042713, rs1042714) genes were investigated. The TT genotype for the COMT rs9332377 gene was highly associated with the presence of muscular TMD ( P = 0.03). With respect to the ADRB2 gene, the non-polymorphic AA genotype in the rs1042713 region was more prevalent in the articular TMD group than in the muscular TMD group ( P = 0.05). This study supports the hypothesis that alterations in the COMT and ADRB2 genes influence the muscular pathophysiology.

The present study aimed to evaluate the influence of manual torque (10 Ncm) versus clinical torque (30 Ncm), which is recommended by the manufacturer, on the total length of morse cone implant abutments. Twenty specimens were prepared and distributed into two groups: group 1 with ten analogs for morse cone type implant, and group 2 with ten morse type implants, size 4.3 × 15 cm. In each group, the distance between the implant platform to the top of the prosthetic abutment (abutment height) was measured and subjected to a torque of 10 Ncm. Then, the 30 Ncm torque was applied to the same abutment, and abutment height was measured. The distance between the top of the abutment and the implant/analog base was measured. In order to verify the clinical reproducibility of the experiment, comparisons between the abutment height of the analog at 10 Ncm and the implant at 30 Ncm were performed, showing a greater discrepancy in torque for the 10 Ncm analog (p &lt, 0.05). In order to verify if the change in the laboratory protocol from 10 to 30 Ncm could minimize the differences in the height of the prosthetic abutments, the abutment height in groups 1 and 2 was compared with 30 Ncm, and no significant difference was observed (p &gt, 0.05). The data indicated that the manual torque and the torque recommended by the manufacturer influence the total length of the prosthetic abutments of morse cone implants.

Objective: Report a case of passive geographic tongue that became active due to the presence of severe stress associated to the coronavirus disease 2019 (COVID-19) pandemic. Case details: In January 2019, a 42-years-old Caucasian female was included in a survey on geographic tongue (GT). At that time, although she reported having the condition, on intraoral examination, no atrophic areas or whitish margins were observed on her tongue (classic clinical signs of GT). In November 2020, she was contacted again and reported changes in her GT. At this time, the lateral borders of her tongue presented erythematous areas surrounded by whitish margins. The research participant also reported that she was under severe stress due to the pandemic. Final considerations: This case reported has demonstrated consistency with literature data regarding the onset, worsening of symptoms and changes in clinical aspects of geographic tongue associated with emotional and psychological factors. To ensure that changes in clinical aspects of GT may be related to pandemic stress, further research of oral manifestations of COVID-19 is needed.

RESUMO: Para competir nos diversos níveis, é imprescindível que os atletas estejam saudáveis, sendo a saúde bucal parte integrante da saúde geral e do bem-estar. Contudo, durante a prática esportiva há a exposição de diversos fatores de risco, que podem se relacionar com o desenvolvimento de dor orofacial e variados tipos de desordens temporomandibulares (DTM). OBJETIVO: Revisar a literatura sobre os possíveis fatores de risco envolvidos no desenvolvimento das DTM em atletas, assim como apresentar a prevalência dessas desordens nas diversas modalidades esportivas. MÉTODOS: As bases de dados consultadas foram PubMed, BVS e Scielo por meio dos descritores “temporomandibular disorder”, “athletes” e “sport”. A seleção foi realizada no período de 2000 a 2020. Os resultados dos estudos mostram que macrotraumas; aumento na tolerância à dor; estresse psicológico; realização de hábitos parafuncionais; sobrecarga músculo articular devido a treinos constantes; alterações hormonais e genéticas podem aumentar as chances de atletas desenvolverem DTM, sendo que a prevalência dessa desordem varia de acordo com a modalidade esportiva, assim como sexo e idade do atleta. A presença da sintomatologia dolorosa causada pelas DTM impacta não apenas no desempenho, mas principalmente na qualidade de vida do indivíduo. Quando a dor se cronifica, pode haver a associação com a presença de dor em outras articulações do corpo, relacionadas aos mecanismos de sensibilização periférica e central. CONCLUSÃO: Atletas acometidos por DTM, passam a apresentar baixa resistência a tarefas motoras que envolvem demanda e resistência. Uma abordagem interdisciplinar deve ser estimulada, de forma que o manejo e orientação destes pacientes, seja feito precocemente, evitando a piora na qualidade de vida e comprometimento em atividades esportivas.

Dentistry constitutes the basic nucleus of professionals of higher level of health in Brazil with one of the largest concentrations of dentists per capita in the world. However, the genetic in dentistry in Brazil is explored, basically, in research field. Future actions need to be performed in order to deep the whole knowledge about diagnosis and treatment of diseases with genetic basis in dentistry, in Brazil.

This study aimed to evaluate the correlation between epidermal growth factor (EGF) and receptor (EGFR) levels in different clinical stages of dental implant rehabilitation and trace mucositis development’s biological profile. Thirty-six participants from the Specialization in Implant Dentistry, Universidade Federal Fluminense, Brazil, were included in the study and underwent sample collection: inside the alveolar socket, immediately before implant placement (Group 1, n = 10), at the peri-implant crevicular fluid (PICF) during reopening (Group 2, n = 10), PICF from healthy peri-implant in function (Group 3, n = 8), and PICF from mucositis sites (Group 4, n = 18). Quantitative polymerase chain reaction (PCR) evaluated EGF/EGFR gene expression using the SYBR Green Master Mix detection system. The results showed that EGF expression in the peri-implant crevicular fluid was statistically different. There was a higher EGF expression for group C (peri-implant health) (p = 0.04) than for the other groups. Regarding EGFR, there was no statistical difference among the groups (p = 0.56). It was concluded that low levels of EGF gene expression in the peri-implant crevicular fluid are related to the development of peri-implant mucositis and the absence of mucosae sealing. There was no correlation between EGFR gene expression with health or mucositis.

Aim This study aimed to evaluate the prevalence of S. pneumoniae colonization in three different sites in healthy adults: nasopharynx, oropharynx and gingival sulcus. Methods Two-hundred and sixty five adults, aged 20–60 years, who attended dental clinics in one public university (n = 106) and one military institution (n = 159) were enrolled in this study. Pneumococcal detection was performed by direct culture (DC) and PCR for lytA gene after a broth enrichment step. Capsular types were determined by sequential multiplex PCR. Results We identified 18 (6.8%) pneumococcal carriers among 265 adults by PCR, but only one (0.4%) pneumococcal strain was isolated by DC method. Oropharynx (17; 6.4%) was the main source of S. pneumoniae. Colonization of gingival sulcus and nasopharynx was found in 4 (1.5%) and 2 (0.8%) adults, respectively. Nine distinct capsular types were detected from 9 adults and co-colonization with 2 serotypes was confirmed in 4 (1.5%) subjects. Factors associated with carriage were being females, low level of schooling, non-military and regular medication. We observed a low (6.8%) pneumococcal carriage prevalence, but oropharyngeal samples yielded more sensitive results, especially by the PCR-based detection methodology. Conclusion Gingival sulcus was found to be a possible reservoir for S. pneumoniae independently of the oropharynx or nasopharynx colonization.

The pain from temporomandibular disorder (TMD) is often associated with physical symptoms of other chronic pain disorders and comorbidities, such as generalised muscle and joint pain. However, this association is not widely studied. To evaluate the prevalence of comorbid pain in joints, specifically in the knees, hips, ankles, shoulders, wrists and elbows, in individuals with and without TMD. We evaluated 337 patients from a public hospital in the city of Rio de Janeiro, Brazil. The Research Diagnostic Criteria for TMD questionnaire were used for the diagnosis of TMD. To assess the presence of other joint pain, the patients were asked to answer questions considering: the presence of pain in the knee, hip, ankle, shoulder, wrist and elbow joints and time duration of pain. Individuals with TMD are 5·5 times more likely to present with other joint pain compared with those without the disorder. TMD muscle disorders were most associated with a higher number of pain at the other locations. There was a significant association between the presence of pain at the other locations, muscle (P

Objective: To evaluate the association between polymorphisms in genes and comorbid presence of arthralgias and TMD.Methods: This is a case-control study. The groups formed were individuals ...

Objective: To report a clinical cases of uterus didelphys and successful pregnancy. Case details: Case 1: patient with high-risk pregnancy due to Hypothyroidism, overweight and the presence of uterus didelphys. In the past history: anemia, the patient reported menstrual irregularity. The pregnancy went according to the proper procedures, however, a significant increase in weight was observed, as a consequence, gestational arterial hypertension was detected. Cesarean delivery occurred at 37 weeks and three days without complications. Case 2: patient diagnosed with uterus didelphy at 65, history of treatment for becoming pregnant, intense menstrual flow, cramps and two successful pregnancies. However, the patient was not aware of the uterine malformation and there was no information about this fact in the medical record. All patients included in this study provided informed consent. Final consideration: Despite pregnancy being considered the presence of the uterus didelphys, the pregnancies went according to the appropriate procedures considering the guidelines recommended by the Ministry of Health (MS). Therefore, the therapeutic approach applied to clinical cases was successful.

Objetivo: Avaliar se os níveis de vitamina D séricos tem correlação com os níveis intraósseos de OPG (Osteoprotegerina) / RANKL (ligante do receptor ativador do fator nuclear kappa Beta (NF-kB)). Métodos: Oito participantes de pesquisa selecionados no curso de especialização em Implantodontia da Universidade Federal Fluminense foram incluídos neste estudo piloto e divididos em 2 grupos: 1) grupo controle (nível sérico adequado de vitamina D; n=5) e 2) grupo teste (insuficiência de vitamina D sérica; n=3). Amostras de sangue intraósseo foram coletadas no alvéolo cirúrgico, no momento da instalação do implante, e submetidas ao isolamento de RNA total usando o reagente Trizol®. A reação de transcriptase reversa foi realizada para síntese do DNA complementar (cDNA) usando 300 ng de RNA. A expressão dos genes OPG e RANKL foi avaliada pela reação em cadeia da polimerase quantitativa (RT-PCR), utilizando o sistema de detecção SYBR Green Master Mix. Resultados: Não foram observadas diferenças estatísticas entre os grupos, em relação à análise de expressão gênica de OPG/RANKL (p=0.78), OPG (p=0.5) e RANKL (p=0.5). Conclusão: Os resultados deste estudo piloto não evidenciaram correlação entre alterações nos níveis séricos de vitamina D e marcadores da remodelação óssea.

O bruxismo é definido como uma atividade repetitiva dos músculos mastigatórios caracterizada pelo ranger e/ou apertar de dentes com diferentes manifestações circadianas. Devido à complexidade da condição e a variedade de distúrbios associados ressalta-se a necessidade de um conhecimento detalhado sobre possíveis fatores individuais a fim de direcionar a conduta específica para consequências do bruxismo. A obtenção de dados genético-moleculares para estudo da possível relação entre genética e bruxismo acarretaria em um melhor diagnóstico e tratamento dos bruxômanos. Dessa forma, o presente estudo buscou elucidar essa relação por meio de uma revisão de literatura. Estudos atuais mostraram indícios da influência de genes da via dopaminérgica e seratoninérgica no desenvolvimento do bruxismo. Com base em relações que possam existir entre fatores genéticos e a origem da condição, mostra-se necessária a ampliação do campo de pesquisa genética-molecular no objetivo de elucidar mecanismos associados e/ou desencadeantes da parafunção, possibilitando diagnósticos e tratamentos precisos.

Objectives To investigate whether genetic variants can be correlated with tendinopathy in elite male volleyball athletes. Design Case-control study. Methods Fifteen single nucleotide polymorphisms within BMP4 , FGF3 , FGF10 , FGFR1 genes were investigated in 138 elite volleyball athletes, aged between 18 and 35 years, who undergo 4–5h of training per day: 52 with tendinopathy and 86 with no history of pain suggestive of tendinopathy in patellar, Achilles, shoulder, and hip abductors tendons. The clinical diagnostic criterion was progressive pain during training, confirmed by magnetic resonance image. Genomic DNA was obtained from saliva samples. Genetic markers were genotyped using TaqMan real-time PCR. Chi-square test compared genotypes and haplotype differences between groups. Multivariate logistic regression analyzed the significance of covariates and incidence of tendinopathy. Results Statistical analysis revealed participant age ( p =0.005) and years of practice ( p =0.004) were risk factors for tendinopathy. A significant association between BMP4 rs2761884 ( p =0.03) and tendinopathy was observed. Athletes with a polymorphic genotype have 2.4 times more susceptibility to tendinopathy (OR=2.39; 95%CI=1.10–5.19). Also, association between disease and haplotype TTGGA in BMP4 ( p =0.01) was observed. The FGF3 TGGTA haplotype showed a tendency of association with tendinopathy ( p =0.05), and so did FGF10 rs900379. FGFR1 showed no association with disease. Conclusions These findings indicate that haplotypes in BMP4 and FGF3 genes may contribute to the tendon disease process in elite volleyball athletes.

tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p

A desordem temporomandibular (DTM) e uma doenca multifatorial que afeta a articulacao temporomandibular (ATM), musculos mastigatorios e tecidos adjacentes, ocasionando dor cronica na maioria dos pacientes. Apesar da DTM ter origem inicial nao inflamatoria, a progressao da doenca e acompanhada por alteracoes inflamatorias secundarias, determinadas pela interacao entre moleculas e celulas, tais como interleucinas, via Wnt, metaloproteinases e fator de necrose tumoral tipo- α. Alteracoes nos niveis dessas moleculas podem interferir nessa resposta, oferecendo maior protecao ou susceptibilidade ao desenvolvimento da doenca. Essa resposta inflamatoria e coordenada pela caracteristica genetica, por isso, e studos recentes tentam explorar o padrao genetico individual como forma de justificar a etiologia das DTM. Polimorfismos nos genes responsaveis pela resposta inflamatoria tem mostrado serem potenciais para futuros procedimentos clinicos em odontologia. Sendo assim, o objetivo desta revisao de literatura foi analisar a correlacao entre a caracteristica genetica individual e o desenvolvimento de DTM. Baseando-se nos artigos revisados, este estudo demonstrou que a caracteristica genetica pode ter correlacao com o desenvolvimento de DTM. No entanto, estudos incluindo maior numero de pacientes e diagnostico mais preciso da DTM ainda sao necessarios para confirmar esta correlacao. Acredita-se que futuros metodos diagnosticos e terapeuticos poderao ter como base a caracteristica genetica do individuo de acordo com a identificacao das regioes polimorficas. Tais metodos poderao propiciar o desenvolvimento de tratamentos padronizados para cada tipo de paciente, identificacao de pacientes de risco e protocolos de proservacao.

The aim of this study was to evaluate the association between genetic polymorphisms and the comorbid presence of chronic systemic arthralgia in patients with articular temporomandibular disorders (TMD). Subjects were evaluated for the presence of TMD and asked about the presence of chronic joint pain. Four groups were included in the study: articular TMD and systemic arthralgia (n=85), no articular TMD and systemic arthralgia (n=82), articular TMD and no systemic arthralgia (n=21), no articular TMD and no systemic arthralgia (control, n=72). A total of 14 single nucleotide polymorphisms in the OPG, RANK, and RANKL genes were investigated. In the statistical analysis, a P-value of0.05 was considered significant. For the OPG gene, an association was observed between the group with chronic arthralgia and joint TMD and the control group (P=0.04). There was also a tendency towards an association of the haplotype CGCCAA with an increased risk of developing chronic joint pain, even in the absence of TMD (P=0.06). For the RANK gene, the AGTGC haplotype was associated with the lowest risk of presenting chronic joint pain in individuals without TMD (P=0.03). This study supports the hypothesis that changes in the OPG and RANK genes influence the presence of chronic joint pain in individuals with and without TMD.

Background Alternative pretreatment strategies of dentin and adhesionare constantly being developed and studied with the goal of improving the adhesion of resin restorative materials with this tissue. The objectives of the present study were to evaluate the ability of airborne-particle abrasion (APA) with aluminum oxide on dentin to remove the smear layer and the effects produced on the dentin microstructure. Materials and methods The phosphoric acid (PA) was used for a comparison. For that, 20 human third molars were randomly allocated into two experimental groups, according to the dentin pretreatment method used: G1 (N = 10) – PA, G2 (N = 10) – APA. For dentin surface analyses, an environmental scanning electron microscope (ESEM) was employed to observe dentin surfaces before and after the procedures. Before pretreatment, the specimens of both groups were smear covered. Results After pretreatment, the G1 images revealed dentin tubule orifices opened, enlarged and some erosive effects. (G2) exposed tubule orifices without enlargement, but crack-like alterations were observed on the surfaces. In this way, APA with aluminum oxide was able to remove the smear layer. Conclusion The influences of the dentin roughness on adhesion and the consequences on dentin integrity and hardness need further investigations. Clinical significance A good conditioning of the dentin before cementation is necessary in order to obtain a satisfactory rehabilitation in adhesive dentistry. So, it is necessary to know all methods to do it. How to cite this article Rafael CF, Quinelato V, Morsch CS, DeDeus G, Reis CM. Morphological Analysis of Dentin Surface after Conditioning with Two Different methods: Chemical and Mechanical. J Contemp Dent Pract 2016;17(1):58-62.

Purpose The high prevalence of painful temporomandibular disorders (TMDs) in women suggests that estrogen and its receptors play a fundamental etiologic role in the development of this joint pathology through complex action mechanisms. The aim of this study was to evaluate the possible association between polymorphisms in the ESR1 (estrogen receptor-1) and ESRRB (estrogen-related receptor-β) genes and the risk of simultaneous development of TMDs and pain in other joints in the body. Materials and Methods All participants were clinically evaluated for the presence of TMD (Research Diagnostic Criteria for TMD) and asked about the presence of chronic joint pain. The control group consisted of 72 patients without TMD and without pain. Participants with arthralgia were divided into 3 groups: with muscular TMD (n = 42), with articular TMD (n = 16), and without TMD and with systemic arthralgia (n = 82). Eight single-nucleotide polymorphisms in the ESR1 (rs12154178, rs1884051, rs2273206, rs7774230) and ESRRB (rs1676303, rs4903399, rs10132091, rs7151924) genes were investigated. The χ2 test and Student t and Mann-Whitney tests were used to assess the relevance of nominal and continuous variables, respectively. A P value less than .05 was considered significant. Results The TT (timin/timin) genotype for the ESR1 (rs2273206) gene was strongly associated with the risk of developing muscle TMDs and temporomandibular joint pain (P = .04). For the ESRRB (rs1676303) gene, an association was observed between the CC (cytosine/cytosine) genotype and the presence of articular TMDs associated with other chronic arthralgia (P = .02). These results were confirmed by the increased risk of developing articular TMDs associated with the C allele (P = .04). Conclusions This study supports the hypothesis that changes in the ESR1 and ESRRB genes influence the presence of TMDs associated with chronic joint pain.

Subjects susceptible to chronic periodontitis (CP) show a high risk for the development of peiimplantitis (PI). Both diseases are multifactorial, presenting similarities in their pathophysiology and polygenic profile. MMP-13 (matrix metalloproteinases 13/ collagenase 3) is a collagenolytic enzyme, which expression is induced by TGF beta 3 (transforming growth factor type 3) in human gingival fibroblasts and inhibited by TIMP-2 (tissue inhibitor of metalloproteinase type 2). The aim of this study was to investigate the occurrence of peiimplantitis (PI) in subjects with history of chronic periodontitis (CP) and polymorphisms frequency in MMP13, TIMP2 and TGFB3 genes. One hundred and sixty-three volunteers received dental implant placement were submitted to oral and radiographic examination in order to identify past history of CP or presence of PI. Volunteers were divided into 4 groups: Control (without PI and CP, n=72), CP (with CP and without PI, n=28), PI (with PI and without CP, n=28) and diseased (with CP and PI, n=35). The chi-square test correlated genotypes in specific regions of MMP13 (rs2252070), TIMP2 (rs7501477) and TGFB3 (rs2268626) genes, considering the interaction between CP and PI. The results showed that volunteers with CP had 3.2 times more susceptibility to develop PI (p=0.0004) compared to those without CP. No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes. Resumo Indivíduos susceptíveis à periodontite crônica (CP) apresentam alto risco para o desenvolvimento de periimplantite (PI). Ambas doenças são multifatoriais e apresentam similaridades na patofisiologia e perfil poligênico. A MMP-13 (metaloproteinase da matriz tipo 13) é uma enzima colagenolítica cuja expressão é induzida por TGF beta 3 (fator transformador do crescimento tipo 3) nos fibroblastos gengivais humanos e inibida por TIMP-2 (inibidor tecidual de metaloproteinase tipo 2). O objetivo deste estudo foi investigar a ocorrência de periimplantite em sujeitos com periodontite crônica e a frequência dos polimorfismos nos genes MMP13, TIMP2 e TGFB3. Cento e sessenta e três voluntários submetidos à instalação de implantes endósseos foram analisados clínica e radiograficamente quanto à presença de histórico de CP e PI, sendo divididos em 4 grupos: Controle (sem história de CP e PI, n=72), CP (com CP e sem PI, n=28), PI (com PI e sem CP, n=28) e Doentes (com CP e PI, n=35). O teste do qui-quadrado correlacionou os genótipos nas regiões dos genes MMP13 (rs2252070), TIMP2 (rs7501477) e TGFB3 (rs2268626), considerando a interação entre CP e PI. Os resultados mostraram que voluntários com CP possuem 3.2 vezes mais chances de desenvolver PI (p=0.0004) comparados aos sem CP. Nenhuma associação significativa foi observada entre os genes MMP13, TIMP2 e TGFB3 e CP ou PI. A CP é um fator de risco ao desenvolvimento de PI, no entanto, não há associação entre ambas as doenças com polimorfismos nos genes MMP13, TIMP2 e TGFB3.

Temporomandibular disorders (TMD) are associated with comorbidity. Shoulder pain is among the symptoms associated with TMD. The purpose of this study was to investigate the association between TMD and rotator cuff disease (RCD) and related genetic aspects. All subjects underwent orofacial and shoulder examinations. The control group comprised 30 subjects with no pain. Affected subjects were divided into three groups: RCD (TMD-free, n=16), TMD (RCD-free, n=13), and TMD/RCD (patients with both RCD and TMD, n=49). A total of eight single nucleotide polymorphisms in the ESRRB gene were investigated. A chemiluminescent immunoassay was used to measure estradiol levels. Surface electromyography recorded head and cervical muscle activity. The χ(2) test and Student t-test/Mann-Whitney test were used to assess the significance of nominal and continuous variables. A P-value of

The muscular Disorders are usually present in the Disfunção Temporomandibular (DTM). The main symptoms includes pain and swelling usually originated f rom t issue i njury a nd i nflammation. T He treatment of DTM can be relied on support treatment for relief of symptoms or definitive elimination of the causes and perpetuate factors. The use of anti- -inflammatory (AINEs), muscle relaxants, occlusal plane, herbal with anti-inflammatory action as Arnica montana is suitable as treatment. Arnica Montana has been used for reduction of inflammation and pain caused by sprain, bruises and wounds. Its main action is inhibition of the cellular transcription factor NF-kB. As desordens musculares geralmente estão presentes na Disfunção Temporomandibular (DTM). Sua principal sintomatologia é dor, edema, proveniente de injúria tecidual e inflamação. O tratamento das DTMs consiste em tratamento suporte para alívio da sintomatologia e “definitivo” para eliminar as causas e fatores que são perpetuantes. O uso de antiinflamatórios (AINEs), relaxantes musculares, placas oclusais, fitoterápicos com ação antiinflamatória como Arnica Montana são indicados como tratamento suporte. A Arnica montana tem sido usada para redução de inflamação e dor causada por entorse, contusões e ferimentos. Sua principal ação é inibição da ativação do fator de transcrição celular NF-kB.

Background There is evidence for a genetic contribution to chronic periodontitis. In this study, we conducted a genome wide association study among 866 participants of the University of Pittsburgh Dental Registry and DNA Repository, whose periodontal diagnosis ranged from healthy (N = 767) to severe chronic periodontitis (N = 99). Methods Genotypingi of over half-million single nucleotide polymorphisms was determined. Analyses were done twice, first in the complete dataset of all ethnicities, and second including only samples defined as self-reported Whites. From the top 100 results, twenty single nucleotide polymorphisms had consistent results in both analyses (borderline p-values ranging from 1E-05 to 1E-6) and were selected to be tested in two independent datasets derived from 1,460 individuals from Porto Alegre, and 359 from Rio de Janeiro, Brazil. Meta-analyses of the Single nucleotide polymorphisms showing a trend for association in the independent dataset were performed. Results The rs1477403 marker located on 16q22.3 showed suggestive association in the discovery phase and in the Porto Alegre dataset (p = 0.05). The meta-analysis suggested the less common allele decreases the risk of chronic periodontitis. Conclusions Our data offer a clear hypothesis to be independently tested regarding the contribution of the 16q22.3 locus to chronic periodontitis.