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1. MPXV DNA kinetics in bloodstream and other body fluids samples

Author

Meschi, Silvia, Colavita, Francesca, Carletti, Fabrizio, Mazzotta, Valentina, Matusali, Giulia, Specchiarello, Eliana, Ascoli Bartoli, Tommaso, Mondi, Annalisa, Minosse, Claudia, Giancola, Maria Letizia, Pinnetti, Carmela, Valli, Maria Beatrice, Lapa, Daniele, Mizzoni, Klizia, Sullivan, David J., Ou, Jiangda, Focosi, Daniele, Girardi, Enrico, Nicastri, Emanuele, Antinori, Andrea, and Maggi, Fabrizio

Published
2024
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3. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital

Author

Abbonizio, Maria Alessandra, Abdeddaim, Amina, Agostini, Elisabetta, Agrati, Chiara, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antinori, Andrea, Antonica, Maria Assunta, Antonini, Mario, Bartoli, Tommaso Ascoli, Baldini, Francesco, Barbaro, Raffaella, Bartolini, Barbara, Bellagamba, Rita, Benigni, Martina, Bevilacqua, Nazario, Biava, Gianluigi, Bibas, Michele, Bordi, Licia, Bordoni, Veronica, Boumis, Evangelo, Branca, Marta, Buonomo, Rosanna, Busso, Donatella, Camici, Marta, Campioni, Paolo, Canichella, Flaminia, Capobianchi, Maria Rosaria, Capone, Alessandro, Caporale, Cinzia, Caraffa, Emanuela, Caravella, Ilaria, Carletti, Fabrizio, Castilletti, Concetta, Cataldo, Adriana, Cerilli, Stefano, Cerva, Carlotta, Chiappini, Roberta, Chinello, Pierangelo, Cianfarani, Maria Assunta, Ciaralli, Carmine, Cimaglia, Claudia, Cinicola, Nicola, Ciotti, Veronica, Cicalini, Stefania, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, De Angelis, Alessia, De Angelis, Giada, De Palo, Maria Grazia, De Zottis, Federico, Di Bari, Virginia, Di Lorenzo, Rachele, Di Stefano, Federica, D’Offizi, Gianpiero, Donno, Davide, Evangelista, Francesca, Faraglia, Francesca, Farina, Anna, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Fusetti, Matteo, Galati, Vincenzo, Gagliardini, Roberta, Gallì, Paola, Garotto, Gabriele, Gaviano, Ilaria, Tekle, Saba Gebremeskel, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Granata, Guido, Greci, Maria Cristina, Grilli, Elisabetta, Grisetti, Susanna, Gualano, Gina, Iacomi, Fabio, Iaconi, Marta, Iannicelli, Giuseppina, Inversi, Carlo, Ippolito, Giuseppe, Lalle, Eleonora, Lamanna, Maria Elena, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Libertone, Raffaella, Lionetti, Raffaella, Liuzzi, Giuseppina, Loiacono, Laura, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Maffongelli, Gaetano, Marani, Alessandra, Marchioni, Luisa, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Mastrobattista, Annelisa, Mastrorosa, Ilaria, Matusali, Giulia, Mazzotta, Valentina, Mencarini, Paola, Meschi, Silvia, Messina, Francesco, Micarelli, Sibiana, Mogavero, Giulia, Mondi, Annalisa, Montalbano, Marzia, Montaldo, Chiara, Mosti, Silvia, Murachelli, Silvia, Musso, Maria, Nardi, Michela, Navarra, Assunta, Nicastri, Emanuele, Nocioni, Martina, Noto, Pasquale, Noto, Roberto, Oliva, Alessandra, Onnis, Ilaria, Ottou, Sandrine, Palazzolo, Claudia, Pallini, Emanuele, Palmieri, Fabrizio, Palombi, Giulio, Pareo, Carlo, Passeri, Virgilio, Pelliccioni, Federico, Penna, Giovanna, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pinnetti, Carmela, Pisciotta, Maria, Piselli, Pierluca, Pittalis, Silvia, Pontarelli, Agostina, Proietti, Costanza, Puro, Vincenzo, Ramazzini, Paolo Migliorisi, Rianda, Alessia, Rinonapoli, Gabriele, Rosati, Silvia, Rubino, Dorotea, Rueca, Martina, Ruggeri, Alberto, Sacchi, Alessandra, Sampaolesi, Alessandro, Sanasi, Francesco, Santagata, Carmen, Scarabello, Alessandra, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Scorzolini, Laura, Stazi, Giulia, Strano, Giacomo, Taglietti, Fabrizio, Taibi, Chiara, Taloni, Giorgia, Nardi, Tetaj, Tonnarini, Roberto, Topino, Simone, Tozzi, Martina, Vaia, Francesco, Vairo, Francesco, Valli, Maria Beatrice, Vergori, Alessandra, Vincenzi, Laura, Visco-Comandini, Ubaldo, Vita, Serena, Vittozzi, Pietro, Zaccarelli, Mauro, Zanetti, Antonella, Zito, Sara, Lorenzini, Patrizia, Di Gennaro, Francesco, and Girardi, Enrico

Published
2021
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4. Enterovirus and Paraechovirus Meningitis in Neonates: Which Is the Difference?

Author

Picone, Simonetta, Mondì, Vito, Di Palma, Federico, Valli, Maria Beatrice, Rueca, Martina, Bedetta, Manuela, and Paolillo, Piermichele

Subjects
LEUCOCYTES, MENINGITIS, RNA virus infections, LABORATORIES, NEURAL development, BRAIN, CALCITONIN, LYMPHOCYTES, TRANSCRANIAL Doppler ultrasonography, ENTEROVIRUS diseases, C-reactive protein, CHILDREN
Abstract

Enterovirus (EV) and parechovirus (HPeV) are common viruses in the neonatal period, with similar seasonality and symptomatology. They also are the main causes of aseptic meningitis in newborns and children under 1 year of age. We compared the clinical signs, laboratory data, brain, and neurodevelopmental outcome of 10 infants with HPeV and 8 with EV meningitis. In patients with EV meningitis, serum C-reactive protein (CRP) values were significantly higher than those of patients with HPeV infection. Procalcitonin values were low in both groups. White blood cell (WBC) and lymphocyte values were significantly higher in EV patients. None of the infants had a brain lesion on cerebral ultrasound neither negative neurological outcome. Based solely on symptoms, it is not possible to distinguish HPeV from EV infection. C-reactive protein, WBC, and lymphocyte values might allow the physician to assume EV infection. The gold standard test for diagnosis remains real-time polymerase chain reaction on cerebral spinal fluid. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Molecular Genotyping of Circulating Enterovirus in the Lazio Region from 2012 to 2023.

Author

Rueca, Martina, Vairo, Francesco, Spaziante, Martina, Fabeni, Lavinia, Forbici, Federica, Berno, Giulia, Gruber, Cesare Ernesto Maria, Picone, Simonetta, Ajassa, Camilla, Girardi, Enrico, Maggi, Fabrizio, and Valli, Maria Beatrice

Subjects
MOLECULAR epidemiology, COVID-19 pandemic, VACCINE effectiveness, SYMPTOMS, PUBLIC health
Abstract

Enteroviruses (EVs) are ubiquitous viruses that circulate worldwide, causing sporadic or epidemic infections, typically during the summer and fall. They cause a broad spectrum of illnesses, ranging from an unspecified febrile clinical presentation to a severe illness. EVs are recognized to be the most frequent etiological agents of aseptic meningitis in children. However, as the infection is usually mild and self-limiting, it remains underestimated, and the epidemiology of EVs is poorly understood. To date, no vaccine or effective therapy for all types of enteroviruses is available, and EVs constitute a public health concern. Here, we investigated the molecular epidemiology of EV strains circulating in the Lazio region over a 10-year time span (2012–2023) by using a sequence-typing approach and phylogenetic analysis. The epidemiological trend of EV infection has undergone changes during the SARS-CoV-2 pandemic (2020–2021), which resulted in a modification in terms of the number of diagnosed cases and seasonality. From 2022, the circulation of EVs showed a behavior typical of the pre-pandemic period, although changes in predominantly circulating strains have been noted. Both epidemic and sporadic circulation events have been characterized in the Lazio region. Further analyses are needed to better characterize any strain with higher potential pathogenic power and to identify possible recombinant strains. [ABSTRACT FROM AUTHOR]

Published
2024
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6. 2019-novel Coronavirus severe adult respiratory distress syndrome in two cases in Italy: An uncommon radiological presentation

Author

Abbonizio, Maria Alessandra, Agrati, Chiara, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antonini, Mario, Barbaro, Raffaella, Bartolini, Barbara, Benigni, Martina, Bevilacqua, Nazario, Bordi, Licia, Bordoni, Veronica, Branca, Marta, Campioni, Paolo, Capobianchi, Maria Rosaria, Caporale, Cinzia, Caravella, Ilaria, Carletti, Fabrizio, Castilletti, Concetta, Chiappini, Roberta, Ciaralli, Carmine, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, Angelis, Alessia De, Angelis, Giada De, Lorenzo, Rachele Di, Stefano, Federica Di, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Gallì, Paola, Garotto, Gabriele, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Greci, Maria Cristina, Ippolito, Giuseppe, Lalle, Eleonora, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Marani, Alessandra, Marchioni, Luisa, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Matusali, Giulia, Meschi, Silvia, Montaldo, Francesco Messina Chiara, Murachelli, Silvia, Nicastri, Emanuele, Noto, Roberto, Palazzolo, Claudia, Pallini, Emanuele, Passeri, Virgilio, Pelliccioni, Federico, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pisciotta, Maria, Pittalis, Silvia, Proietti, Costanza, Puro, Vincenzo, Rinonapoli, Gabriele, Rueca, Martina, Sacchi, Alessandra, Sanasi, Francesco, Santagata, Carmen, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Scorzolini, Laura, Stazi, Giulia, Vaia, Francesco, Vairo, Francesco, Valli, Maria Beatrice, Di Stefano, Federica, Eskild, Petersen, and Zumla, Alimuddin

Published
2020
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8. Pharyngo-tonsillar involvement of Mpox in a cohort of men who have sex with men (MSM): A serious risk of missing diagnosis.

Author

Pinnetti, Carmela, Mondi, Annalisa, Mazzotta, Valentina, Vita, Serena, Carletti, Fabrizio, Aguglia, Camilla, Beccacece, Alessia, Vergori, Alessandra, Gagliardini, Roberta, Specchiarello, Eliana, Ascoli Bartoli, Tommaso, Baldini, Francesco, Giancola, Maria Letizia, Valli, Maria Beatrice, D'Abramo, Alessandra, Gebremeskel Teklè, Saba, Fontana, Carla, Garbuglia, Anna Rosa, Girardi, Enrico, and Maggi, Fabrizio

Abstract

During the 2022-outbreak, peculiar clinical presentations of Mpox have been described, some of which can make the diagnosis of the disease extremely challenging. Here we report a case series of fourteen patients with Mpox pharynogotonsillar involvement (PTI) seen at National Institute for Infectious Diseases, " Lazzaro Spallanzani ", in Rome, Italy from May to September 2022. All included patients were men who have sex with men (median age 38 years) reporting unprotected sex within three weeks from symptoms onset. Seven out of fourteen patients needed hospitalization due to uncontrolled pain, reduced airspace and difficulty swallowing, of whom five were effectively treated with tecovirimat or cidofovir. The remaining two patients were treated with symptomatic drugs. The typical Mpox muco-cutaneous manifestations were not observed simultaneously with PTI in three patients, two of whom developed the lesions after several days, while one never manifested them. Polymerase Chain Reaction (PCR) for Mpox virus was positive in oropharyngeal swab, saliva and serum. Although PTI occurs in only a small percentage of Mpox cases, its diagnosis is of utmost importance. In fact, this localization, if not identified, could lead to serious complications in the absence of early antiviral treatment and to missed diagnosis with an increased risk of disease transmission. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Genomic and Epidemiologic Surveillance of SARS-CoV-2 in the Pandemic Period: Sequencing Network of the Lazio Region, Italy.

Author

Rueca, Martina, Berno, Giulia, Agresta, Alessandro, Spaziante, Martina, Gruber, Cesare Ernesto Maria, Fabeni, Lavinia, Giombini, Emanuela, Butera, Ornella, Barca, Alessandra, Scognamiglio, Paola, Girardi, Enrico, Maggi, Fabrizio, Valli, Maria Beatrice, and Vairo, Francesco

Subjects
COVID-19 pandemic, PUBLIC health surveillance, COVID-19, INFECTIOUS disease transmission, NUCLEOTIDE sequencing, GENOMICS
Abstract

Since the beginning of the COVID-19 pandemic, large-scale genomic sequencing has immediately pointed out that SARS-CoV-2 has rapidly mutated during the course of the pandemic, resulting in the emergence of variants with a public health impact. In this context, strictly monitoring the circulating strains via NGS has proven to be crucial for the early identification of new emerging variants and the study of the genomic evolution and transmission of SARS-CoV-2. Following national and international guidelines, the Lazio region has created a sequencing laboratory network (WGSnet-Lazio) that works in synergy with the reference center for epidemiological surveillance (SERESMI) to monitor the circulation of SARS-CoV-2. Sequencing was carried out with the aims of characterizing outbreak transmission dynamics, performing the genomic analysis of viruses infecting specific categories of patients (i.e., immune-depressed, travelers, and people with severe symptoms) and randomly monitoring variant circulation. Here we report data emerging from sequencing activities carried out by WGSnet-Lazio (from February 2020 to October 2022) linked with epidemiological data to correlate the circulation of variants with the clinical and demographic characteristics of patients. The model of the sequencing network developed in the Lazio region proved to be a useful tool for SARS-CoV-2 surveillance and to support public health measures for epidemic containment. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Enterovirus D68-Associated Acute Flaccid Myelitis in Immunocompromised Woman, Italy

Author

Giombini, Emanuela, Rueca, Martina, Barberi, Walter, Iori, Anna Paola, Inghilleri, Maurizio, Castilletti, Concetta, Scognamiglio, Paola, Vairo, Francesco, Ippolito, Giuseppe, Capobianchi, Maria Rosaria, and Valli, Maria Beatrice

Subjects
Myelitis -- Diagnosis -- Care and treatment -- Case studies, Enterovirus diseases -- Case studies -- Diagnosis -- Care and treatment, Health
Abstract

Enteroviruses are the most common viruses circulating worldwide and lead to a broad spectrum of clinical illnesses: respiratory infection; hand, foot, and mouth disease; acute and chronic cardiac disease; meningitis; [...]

Published
2017
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12. The first case of meningitis associated with SARS-CoV-2 BA.2 variant infection with persistent viremia

Author

D'Abramo, Alessandra, primary, Vita, Serena, additional, Colavita, Francesca, additional, Cimini, Eleonora, additional, Haggiag, Shalom, additional, Maffongelli, Gaetano, additional, Valli, Maria Beatrice, additional, Bevilacqua, Nazario, additional, Corpolongo, Angela, additional, Giancola, Maria Letizia, additional, Maggi, Fabrizio, additional, Agrati, Chiara, additional, and Nicastri, Emanuele, additional

Published
2022
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14. Acute flaccid myelitis and Guillain-Barré syndrome in children: A comparative study with evaluation of diagnostic criteria

Author

Helfferich, J, Roodbol, Joyce, de Wit, Marie-Claire, Brouwer, Oebele F, Jacobs, Bart C., Aberle, Stephan W., Popow-Kraupp, Theresia, Nikolaeva-Glomb, Lubomira, Rainetova, Petra, Midgley, Sofie, Fischer, Thea Kølsen, Simonlatser, Grethel, Savolainen-Kopra, Carita, Lina, Bruno, Schuffenecker, Isabelle, Aubart, Melodie, Gitiaux, Cyril, Antona, Denise, Eis-Hübinger, Anna Maria, Buderus, Stephan, Panning, Marcus, Nowotny, Markus, Kiechle, Lisa, Böttcher, Sindy, Takas, Maria, Farkas, Agnes, Löve, Arthur, Baldanti, Fausto, Capobianchi, Maria Rosaria, Valli, Maria Beatrice, Esposito, Susanna, Pariani, Elena, Binda, Sandro, Neuteboom, Rinze, Pas, Suzan, Benschop, Kimberley, Meijer, Adam, Nordbø, Svein Arne, Hafström, Maria, Dudman, Susanne Gjeruldsen, Pfeiffer, Helle Cecilie Viekilde, Guiomar, Raquel, Palminha, Paula, Costa, Ines, Dias, Andrea Sofia, Tecu, Cristina, Cherciu, Carmen Maria, Lazarevic, Ivana, Filipovic-Vignjevic, Svetlana, Berginc, Natasa, Prinses Beatrix Spierfonds, Neurology, and Immunology

Subjects
Acute flaccid myelitis, Weakness, Pediatrics, medicine.medical_specialty, PARALYSIS, OUTBREAK, FEATURES, Guillain-Barre syndrome, macromolecular substances, Guillain-Barre Syndrome, Guillain–Barré syndrome, DISEASE, Medicine, Humans, Child, reproductive and urinary physiology, OUTCOMES, business.industry, Atomic force microscopy, technology, industry, and agriculture, Diagnostic test, COLORADO, Neuromuscular Diseases, Myelitis, medicine.disease, bacterial infections and mycoses, Guillain-Barré syndrome, diagnosis, Neurology, Cohort, biological sciences, Central Nervous System Viral Diseases, acute flaccid myelitis, Neurology (clinical), medicine.symptom, business, Enterovirus D68, Brighton criteria
Abstract

Background and purpose: Differentiation between acute flaccid myelitis (AFM) and Guillain-Barré syndrome (GBS) can be difficult, particularly in children. Our objective was to improve the diagnostic accuracy by giving recommendations based on a comparison of clinical features and diagnostic criteria in children with AFM or GBS. Methods: A cohort of 26 children with AFM associated with enterovirus D68 was compared to a cohort of 156 children with GBS. The specificity of the Brighton criteria, used for GBS diagnosis, was evaluated in the AFM cohort and the specificity of the Centers for Disease Control and Prevention (CDC) AFM diagnostic criteria in the GBS cohort. Results: Children with AFM compared to those with GBS had a shorter interval between onset of weakness and nadir (3 vs. 8 days, p < 0.001), more often had asymmetric limb weakness (58% vs. 0%, p < 0.001), and less frequently had sensory deficits (0% vs. 40%, p < 0.001). In AFM, cerebrospinal fluid leukocyte counts were higher, whereas protein concentrations were lower. Spinal cord lesions on magnetic resonance imaging were only found in AFM patients. No GBS case fulfilled CDC criteria for definite AFM. Of the AFM cases, 8% fulfilled the Brighton criteria for GBS, when omitting the criterion of excluding an alternate diagnosis. Conclusions: Despite the overlap in clinical presentation, we found distinctive early clinical and diagnostic characteristics for differentiating AFM from GBS in children. Diagnostic criteria for AFM and GBS usually perform well, but some AFM cases may fulfill clinical diagnostic criteria for GBS. This underlines the need to perform diagnostic tests early to exclude AFM in children suspected of atypical GBS. The Prinses Beatrix Spierfonds funded the PhD project of J.R. on GBS in children (project number: W.OR12-04) Sí

Published
2022

15. Molecular Characterization of Whole-Genome SARS-CoV-2 from the First Suspected Cases of the XE Variant in the Lazio Region, Italy.

Author

Rueca, Martina, Giombini, Emanuela, Gramigna, Giulia, Gruber, Cesare Ernesto Maria, Fabeni, Lavinia, Corpolongo, Angela, Mazzotta, Valentina, Corso, Luisella, Butera, Ornella, Valli, Maria Beatrice, Carletti, Fabrizio, Pignalosa, Stefano, Vairo, Francesco, Nicastri, Emanuele, Antinori, Andrea, Girardi, Enrico, Vaia, Francesco, and Maggi, Fabrizio

Subjects
SARS-CoV-2, GENETIC recombination, NUCLEOTIDE sequencing, HOSPITAL patients
Abstract

We report two cases of SARS-CoV-2 recombinant variant XE detected in nasopharyngeal swabs (NPS) of hospitalized patients with no evident epidemiological link in Lazio, Central Italy. Whole-Genome Sequencing (WGS) performed on an Ion Torrent GSS5 platform according to Italian flash surveys showed genomes corresponding to the PANGOLIN unclassified lineage and the Nextclade XE clade. Further analyses were then carried out to investigate more deeply the genetic characteristics of these XE-like sequences. When phylogenetic trees, by using IQ-TREE, were built splitting the genome into two regions according to the putative XE recombination site, the upstream and downstream regions were seen to be clustered near BA.1 and BA.2 sequences, respectively. However, our XE-like sequences clustered separately, with a significant bootstrap, from the classified European and Italian XE strains, although the recombination site between BA.1 and BA.2 was identified at the nucleotide site 11556 by RDP4 software, consistent with the putative XE breakpoint. These findings show the risk of the introduction of novel recombinant variants of SARS-CoV-2 and the existence of XE-like strains, phylogenetically separated, that could make their exact taxonomy difficult. It follows the need for continued SARS-CoV-2 surveillance by WGS. [ABSTRACT FROM AUTHOR]

Published
2022
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17. Risk and predictive factors of prolonged viral RNA shedding in upper respiratory specimens in a large cohort of COVID-19 patients admitted to an Italian reference hospital

Author

Mondi, Annalisa, primary, Lorenzini, Patrizia, additional, Castilletti, Concetta, additional, Gagliardini, Roberta, additional, Lalle, Eleonora, additional, Corpolongo, Angela, additional, Valli, Maria Beatrice, additional, Taglietti, Fabrizio, additional, Cicalini, Stefania, additional, Loiacono, Laura, additional, Di Gennaro, Francesco, additional, D’Offizi, Gianpiero, additional, Palmieri, Fabrizio, additional, Nicastri, Emanuele, additional, Agrati, Chiara, additional, Petrosillo, Nicola, additional, Ippolito, Giuseppe, additional, Vaia, Francesco, additional, Girardi, Enrico, additional, Capobianchi, Maria Rosaria, additional, Antinori, Andrea, additional, Zito, Sara, additional, Abbonizio, Maria Alessandra, additional, Abdeddaim, Amina, additional, Agostini, Elisabetta, additional, Albarello, Fabrizio, additional, Amadei, Gioia, additional, Amendola, Alessandra, additional, Antonica, Maria Assunta, additional, Antonini, Mario, additional, Bartoli, Tommaso Ascoli, additional, Baldini, Francesco, additional, Barbaro, Raffaella, additional, Bartolini, Barbara, additional, Bellagamba, Rita, additional, Benigni, Martina, additional, Bevilacqua, Nazario, additional, Biava, Gianluigi, additional, Bibas, Michele, additional, Bordi, Licia, additional, Bordoni, Veronica, additional, Boumis, Evangelo, additional, Branca, Marta, additional, Buonomo, Rosanna, additional, Busso, Donatella, additional, Camici, Marta, additional, Campioni, Paolo, additional, Canichella, Flaminia, additional, Capone, Alessandro, additional, Caporale, Cinzia, additional, Caraffa, Emanuela, additional, Caravella, Ilaria, additional, Carletti, Fabrizio, additional, Cataldo, Adriana, additional, Cerilli, Stefano, additional, Cerva, Carlotta, additional, Chiappini, Roberta, additional, Chinello, Pierangelo, additional, Cianfarani, Maria Assunta, additional, Ciaralli, Carmine, additional, Cimaglia, Claudia, additional, Cinicola, Nicola, additional, Ciotti, Veronica, additional, Colavita, Francesca, additional, Cristofaro, Massimo, additional, Curiale, Salvatore, additional, D’Abramo, Alessandra, additional, Dantimi, Cristina, additional, De Angelis, Alessia, additional, De Angelis, Giada, additional, De Palo, Maria Grazia, additional, De Zottis, Federico, additional, Di Bari, Virginia, additional, Di Lorenzo, Rachele, additional, Di Stefano, Federica, additional, Donno, Davide, additional, Evangelista, Francesca, additional, Faraglia, Francesca, additional, Farina, Anna, additional, Ferraro, Federica, additional, Fiorentini, Lorena, additional, Frustaci, Andrea, additional, Fusetti, Matteo, additional, Galati, Vincenzo, additional, Gallì, Paola, additional, Garotto, Gabriele, additional, Gaviano, Ilaria, additional, Tekle, Saba Gebremeskel, additional, Giancola, Maria Letizia, additional, Giansante, Filippo, additional, Giombini, Emanuela, additional, Granata, Guido, additional, Greci, Maria Cristina, additional, Grilli, Elisabetta, additional, Grisetti, Susanna, additional, Gualano, Gina, additional, Iacomi, Fabio, additional, Iaconi, Marta, additional, Iannicelli, Giuseppina, additional, Inversi, Carlo, additional, Lamanna, Maria Elena, additional, Lanini, Simone, additional, Lapa, Daniele, additional, Lepore, Luciana, additional, Libertone, Raffaella, additional, Lionetti, Raffaella, additional, Liuzzi, Giuseppina, additional, Lucia, Andrea, additional, Lufrani, Franco, additional, Macchione, Manuela, additional, Maffongelli, Gaetano, additional, Marani, Alessandra, additional, Marchioni, Luisa, additional, Mariano, Andrea, additional, Marini, Maria Cristina, additional, Maritti, Micaela, additional, Mastrobattista, Annelisa, additional, Mastrorosa, Ilaria, additional, Matusali, Giulia, additional, Mazzotta, Valentina, additional, Mencarini, Paola, additional, Meschi, Silvia, additional, Messina, Francesco, additional, Micarelli, Sibiana, additional, Mogavero, Giulia, additional, Mondi, Annalisa, additional, Montalbano, Marzia, additional, Montaldo, Chiara, additional, Mosti, Silvia, additional, Murachelli, Silvia, additional, Musso, Maria, additional, Nardi, Michela, additional, Navarra, Assunta, additional, Nocioni, Martina, additional, Noto, Pasquale, additional, Noto, Roberto, additional, Oliva, Alessandra, additional, Onnis, Ilaria, additional, Ottou, Sandrine, additional, Palazzolo, Claudia, additional, Pallini, Emanuele, additional, Palombi, Giulio, additional, Pareo, Carlo, additional, Passeri, Virgilio, additional, Pelliccioni, Federico, additional, Penna, Giovanna, additional, Petrecchia, Antonella, additional, Petrone, Ada, additional, Pianura, Elisa, additional, Pinnetti, Carmela, additional, Pisciotta, Maria, additional, Piselli, Pierluca, additional, Pittalis, Silvia, additional, Pontarelli, Agostina, additional, Proietti, Costanza, additional, Puro, Vincenzo, additional, Ramazzini, Paolo Migliorisi, additional, Rianda, Alessia, additional, Rinonapoli, Gabriele, additional, Rosati, Silvia, additional, Rubino, Dorotea, additional, Rueca, Martina, additional, Ruggeri, Alberto, additional, Sacchi, Alessandra, additional, Sampaolesi, Alessandro, additional, Sanasi, Francesco, additional, Santagata, Carmen, additional, Scarabello, Alessandra, additional, Scarcia, Silvana, additional, Schininà, Vincenzo, additional, Scognamiglio, Paola, additional, Scorzolini, Laura, additional, Stazi, Giulia, additional, Strano, Giacomo, additional, Taibi, Chiara, additional, Taloni, Giorgia, additional, Nardi, Tetaj, additional, Tonnarini, Roberto, additional, Topino, Simone, additional, Tozzi, Martina, additional, Vairo, Francesco, additional, Vergori, Alessandra, additional, Vincenzi, Laura, additional, Visco-Comandini, Ubaldo, additional, Vita, Serena, additional, Vittozzi, Pietro, additional, Zaccarelli, Mauro, additional, and Zanetti, Antonella, additional

Published
2021
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18. COVID-19 Rapid Antigen Test as Screening Strategy at Points of Entry: Experience in Lazio Region, Central Italy, August–October 2020

Author

Colavita, Francesca, primary, Vairo, Francesco, additional, Meschi, Silvia, additional, Valli, Maria Beatrice, additional, Lalle, Eleonora, additional, Castilletti, Concetta, additional, Fusco, Danilo, additional, Spiga, Giuseppe, additional, Bartoletti, Pierluigi, additional, Ursino, Simona, additional, Sanguinetti, Maurizio, additional, Di Caro, Antonino, additional, Vaia, Francesco, additional, Ippolito, Giuseppe, additional, and Capobianchi, Maria Rosaria, additional

Published
2021
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19. Pharmacokinetics of remdesivir and GS-441524 in two critically ill patients who recovered from COVID-19

Author

Tempestilli, Massimo, Caputi, Priscilla, Avataneo, Valeria, Notari, Stefania, Forini, Olindo, Scorzolini, Laura, Marchioni, Luisa, Ascoli Bartoli, Tommaso, Castilletti, Concetta, Lalle, Eleonora, Capobianchi, Maria R, Nicastri, Emanuele, D’Avolio, Antonio, Ippolito, Giuseppe, Agrati, Chiara, Abbonizio, Maria Alessandra, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antinori, Andrea, Antonini, Mario, Barbaro, Raffaella, Bartolini, Barbara, Benigni, Martina, Bevilacqua, Nazario, Bordi, Licia, Bordoni, Veronica, Branca, Marta, Campioni, Paolo, Capobianchi, Maria Rosaria, Caporale, Cinzia, Caravella, Ilaria, Carletti, Fabrizio, Casetti, Rita, Chiappini, Roberta, Ciaralli, Carmine, Cimini, Eleonora, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, De Angelis, Alessia, De Angelis, Giada, Di Lorenzo, Rachele, Di Stefano, Federica, D’Offizi, Gianpiero, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Gallì, Paola, Garotto, Gabriele, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Grassi, Germana, Greci, Maria Cristina, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Marani, Alessandra, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Matusali, Giulia, Meschi, Silvia, Messina, Francesco, Montaldo, Chiara, Murachelli, Silvia, Noto, Roberto, Palazzolo, Claudia, Pallini, Emanuele, Palmieri, Fabrizio, Passeri, Virgilio, Pelliccioni, Federico, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pisciotta, Maria, Pittalis, Silvia, Proietti, Costanza, Puro, Vincenzo, Rinonapoli, Gabriele, Rueca, Martina, Sacchi, Alessandra, Sanasi, Francesco, Santagata, Carmen, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Stazi, Giulia, Vaia, Francesco, Vairo, Francesco, and Valli, Maria Beatrice

Subjects
0301 basic medicine, Drug, Male, Microbiology (medical), medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), COVID19, media_common.quotation_subject, Critical Illness, 030106 microbiology, Pneumonia, Viral, remdesivir, Loading dose, Gastroenterology, Antiviral Agents, NO, 03 medical and health sciences, Betacoronavirus, Adenosine Triphosphate, Pharmacokinetics, Internal medicine, medicine, Humans, Pharmacology (medical), Pandemics, media_common, EC50, Original Research, Aged, Pharmacology, Lung, Alanine, Nucleoside analogue, business.industry, SARS-CoV-2, COVID-19, Recovery of Function, Prodrug, Adenosine Monophosphate, 030104 developmental biology, medicine.anatomical_structure, Infectious Diseases, Pharmacokinetics, COVID19, remdesivir, Female, business, Coronavirus Infections, medicine.drug
Abstract

Background Remdesivir is a prodrug of the nucleoside analogue GS-441524 and is under evaluation for treatment of SARS-CoV-2-infected patients. Objectives To evaluate the pharmacokinetics of remdesivir and GS-441524 in plasma, bronchoalveolar aspirate (BAS) and CSF in two critically ill COVID-19 patients. Methods Remdesivir was administered at 200 mg loading dose on the first day followed by 12 days of 100 mg in two critically ill patients. Blood samples were collected immediately after (C0) and at 1 (C1) and 24 h (C24) after intravenous administration on day 3 until day 9. BAS samples were collected on Days 4, 7 and 9 from both patients while one CSF on Day 7 was obtained in one patient. Remdesivir and GS-441524 concentrations were measured in these samples using a validated UHPLC-MS/MS method. Results We observed higher concentrations of remdesivir at C0 (6- to 7-fold higher than EC50 from in vitro studies) and a notable decay at C1. GS-441524 plasma concentrations reached a peak at C1 and persisted until the next administration. Higher concentrations of GS-441524 were observed in the patient with mild renal dysfunction. Mean BAS/plasma concentration ratios of GS-441524 were 2.3% and 6.4% in Patient 1 and Patient 2, respectively. The CSF concentration found in Patient 2 was 25.7% with respect to plasma. GS-441524 levels in lung and CNS suggest compartmental differences in drug exposure. Conclusions We report the first pharmacokinetic evaluation of remdesivir and GS-441524 in recovered COVID-19 patients. Further study of the pharmacokinetic profile of remdesivir, GS-441524 and the intracellular triphosphate form are required.

Published
2020
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20. SARS-CoV-2 Early Screening at the Point of Entry: Travelers From Bangladesh to Italy–July 2020

Author

Rueca, Martina, primary, Di Caro, Antonino, additional, Gruber, Cesare Ernesto Maria, additional, Messina, Francesco, additional, Giombini, Emanuela, additional, Valli, Maria Beatrice, additional, Lalle, Eleonora, additional, Lanini, Simone, additional, Vairo, Francesco, additional, Capobianchi, Maria Rosaria, additional, and Bartolini, Barbara, additional

Published
2021
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21. 2019-novel Coronavirus severe adult respiratory distress syndrome in two cases in Italy: An uncommon radiological presentation

Author

Albarello, Fabrizio, primary, Pianura, Elisa, additional, Di Stefano, Federica, additional, Cristofaro, Massimo, additional, Petrone, Ada, additional, Marchioni, Luisa, additional, Palazzolo, Claudia, additional, Schininà, Vincenzo, additional, Nicastri, Emanuele, additional, Petrosillo, Nicola, additional, Campioni, Paolo, additional, Eskild, Petersen, additional, Zumla, Alimuddin, additional, Ippolito, Giuseppe, additional, Abbonizio, Maria Alessandra, additional, Agrati, Chiara, additional, Albarello, Fabrizio, additional, Amadei, Gioia, additional, Amendola, Alessandra, additional, Antonini, Mario, additional, Barbaro, Raffaella, additional, Bartolini, Barbara, additional, Benigni, Martina, additional, Bevilacqua, Nazario, additional, Bordi, Licia, additional, Bordoni, Veronica, additional, Branca, Marta, additional, Capobianchi, Maria Rosaria, additional, Caporale, Cinzia, additional, Caravella, Ilaria, additional, Carletti, Fabrizio, additional, Castilletti, Concetta, additional, Chiappini, Roberta, additional, Ciaralli, Carmine, additional, Colavita, Francesca, additional, Corpolongo, Angela, additional, Curiale, Salvatore, additional, D’Abramo, Alessandra, additional, Dantimi, Cristina, additional, Angelis, Alessia De, additional, Angelis, Giada De, additional, Lorenzo, Rachele Di, additional, Stefano, Federica Di, additional, Ferraro, Federica, additional, Fiorentini, Lorena, additional, Frustaci, Andrea, additional, Gallì, Paola, additional, Garotto, Gabriele, additional, Giancola, Maria Letizia, additional, Giansante, Filippo, additional, Giombini, Emanuela, additional, Greci, Maria Cristina, additional, Lalle, Eleonora, additional, Lanini, Simone, additional, Lapa, Daniele, additional, Lepore, Luciana, additional, Lucia, Andrea, additional, Lufrani, Franco, additional, Macchione, Manuela, additional, Marani, Alessandra, additional, Mariano, Andrea, additional, Marini, Maria Cristina, additional, Maritti, Micaela, additional, Matusali, Giulia, additional, Meschi, Silvia, additional, Montaldo, Francesco Messina Chiara, additional, Murachelli, Silvia, additional, Noto, Roberto, additional, Pallini, Emanuele, additional, Passeri, Virgilio, additional, Pelliccioni, Federico, additional, Petrecchia, Antonella, additional, Pisciotta, Maria, additional, Pittalis, Silvia, additional, Proietti, Costanza, additional, Puro, Vincenzo, additional, Rinonapoli, Gabriele, additional, Rueca, Martina, additional, Sacchi, Alessandra, additional, Sanasi, Francesco, additional, Santagata, Carmen, additional, Scarcia, Silvana, additional, Scognamiglio, Paola, additional, Scorzolini, Laura, additional, Stazi, Giulia, additional, Vaia, Francesco, additional, Vairo, Francesco, additional, and Valli, Maria Beatrice, additional

Published
2020
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23. Hemagglutinin 222 variants in pandemic (H1N1) 2009 virus

Author

Valli, Maria Beatrice, Selleri, Marina, Meschi, Silvia, Zaccaro, Paola, Vincenti, Donatella, Lalle, Eleonora, Capobianchi, Maria Rosaria, and Menzo, Stefano

Subjects
Influenza viruses -- Health aspects -- Genetic aspects -- Research, Genetic variation -- Research -- Genetic aspects -- Health aspects, Health
Abstract

To the Editor: The biologic role of amino acid variants at position 222 of the hemagglutinin (HA) gene of pandemic (H1N1) 2009 virus in severe infections has been extensively discussed. [...]

Published
2011
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24. Down Syndrome patients with COVID-19 pneumonia: A high-risk category for unfavourable outcome

Author

Abbonizio, Agrati, Chiara, Albarello, Fabrizio, Amadei, Gioia, Amendola, Alessandra, Antonini, Mario, Barbaro, Raffaella, Bartolini, Barbara, Benigni, Martina, Bevilacqua, Nazario, Bordi, Licia, Bordoni, Veronica, Branca, Marta, Campioni, Paolo, Capobianchi, Maria Rosaria, Caporale, Cinzia, Caravella, Ilaria, Carletti, Fabrizio, Castilletti, Concetta, Chiappini, Roberta, Ciaralli, Carmine, Colavita, Francesca, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, Angelis, Alessia De, Angelis, Giada De, Lorenzo, Rachele Di, Stefano, Federica Di, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Gallì, Paola, Garotto, Gabriele, Giancola, Maria Letizia, Giansante, Filippo, Giombini, Emanuela, Greci, Maria Cristina, Ippolito, Giuseppe, Lalle, Eleonora, Lanini, Simone, Lapa, Daniele, Lepore, Luciana, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Marani, Alessandra, Marchioni, Luisa, Mariano, Andrea, Marini, Maria Cristina, Maritti, Micaela, Matusali, Giulia, Meschi, Silvia, Montaldo, Francesco Messina Chiara, Murachelli, Silvia, Nicastri, Emanuele, Noto, Roberto, Palazzolo, Claudia, Pallini, Emanuele, Passeri, Virgilio, Pelliccioni, Federico, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pisciotta, Maria, Pittalis, Silvia, Proietti, Costanza, Puro, Vincenzo, Rinonapoli, Gabriele, Rueca, Martina, Sacchi, Alessandra, Sanasi, Francesco, Santagata, Carmen, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Scorzolini, Laura, Stazi, Giulia, Vaia, Francesco, Vairo, Francesco, Valli, Maria Beatrice, Vita, Serena, Di Bari, Virginia, Goletti, Delia, Espinosa, Joaquin, Petracca, Sebastiano, and Palmieri, Fabrizio

Published
2021
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26. Genetic diversity of the haemagglutinin (HA) of human influenza a (H1N1) virus in montenegro: Focus on its origin and evolution

Author

Mugosa, Boban, primary, Vujosevic, Danijela, additional, Ciccozzi, Massimo, additional, Valli, Maria Beatrice, additional, Capobianchi, Maria Rosaria, additional, Lo Presti, Alessandra, additional, Cella, Eleonora, additional, Giovanetti, Marta, additional, Lai, Alessia, additional, Angeletti, Silvia, additional, Scarpa, Fabio, additional, Terzić, Dragica, additional, and Vratnica, Zoran, additional

Published
2016
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27. Prone Positioning and Intravenous Zanamivir may Represent Effective Alternatives for Patients with Severe ARDS Virus A (H1N1) Related Pneumonia in Hospitals with no Access to ECMO

Author

Gristina, Giuseppe, Nardi, Giuseppe, Orazi, Daniela, Lauria, Francesco Nicola, Valli, Maria Beatrice, Lalle, Eleonora, Menzo, Stefano, Riccioni, Luigi, and Camporiondo, Maria Pia

Subjects
Article Subject, viruses, virus diseases, respiratory tract diseases
Abstract

The first patient with influenza A/H1N1-related pneumonia was admitted to an Italian ICU at the end of August 2009. Until then, despite the international alarm, the level of awareness was low and very few Italian hospitals were equipped with ECMOs. Moreover the PCR test for A H1N1 virus was sporadically available and the emergency departments of even the largest institutions could rely only on the rapid test for the urgent screening of patients with pneumonia and respiratory failure. On September 5th, a young and “apparently” previously healthy man, was admitted to our ICU because of a severe ARDS caused by influenza A H1N1 virus. As there was no ECMO available, he was treated with prolonged cycles of prone positioning ventilation. Antiviral treatment was started with Oseltamivir, but as enteral absorption was impaired by paralytic ileus and tube feeding intolerance, Oseltamivir had to be discontinued. Intravenous Zanamivir 1200 mg/day for ten days was therefore prescribed as “off label” antiviral therapy. A bone marrow biopsy allowed the diagnosis of an initial stage of “hairy cells leukaemia.” ARDS related to A/H1N1 influenza was the first sign of the disease in our patient. He did well with complete clearance of the infection from the BAL after 10 days of Zanamivir, although the nasopharyngeal swabs remained positive for ten more days. Prone positioning ventilation may be a life-saver strategy in patients with severe ARDS when ECMO is not immediately available. However, prone positioning ventilation is often associated with severe impairment of the absorption of drugs that require enteral administration via the nasogastric tube. In these cases, intravenous Zanamivir may be an effective alternative strategy.

Published
2010
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29. Frequency of Detection of Upper Respiratory Tract Viruses in Patients Tested for Pandemic H1N1/09 Viral Infection

Author

Nisii, Carla, primary, Meschi, Silvia, additional, Selleri, Marina, additional, Bordi, Licia, additional, Castilletti, Concetta, additional, Valli, Maria Beatrice, additional, Lalle, Eleonora, additional, Lauria, Francesco Nicola, additional, Piselli, Pierluca, additional, Lanini, Simone, additional, Ippolito, Giuseppe, additional, Di Caro, Antonino, additional, and Capobianchi, Maria Rosaria, additional

Published
2010
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31. Transmission in vitro of hepatitis C virus from persistently infected human B-cells to hepatoma cells by cell-to-cell contact

Author

Valli, Maria Beatrice, primary, Serafino, Annalucia, additional, Crema, Annalisa, additional, Bertolini, Luisa, additional, Manzin, Aldo, additional, Lanzilli, Giulia, additional, Bosman, Cesare, additional, Iacovacci, Silvia, additional, Giunta, Sergio, additional, Ponzetto, Antonio, additional, Clementi, Massimo, additional, and Carloni, Guido, additional

Published
2005
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33. Transmission in vitro of hepatitis C virus from persistently infected human B‐cells to hepatoma cells by cell‐to‐cell contact

Author

Valli, Maria Beatrice, Serafino, Annalucia, Crema, Annalisa, Bertolini, Luisa, Manzin, Aldo, Lanzilli, Giulia, Bosman, Cesare, Iacovacci, Silvia, Giunta, Sergio, Ponzetto, Antonio, Clementi, Massimo, and Carloni, Guido

Abstract

Virus cell‐to‐cell spread has been reported for many different viruses and may contribute to pathogenesis of viral disease. The role played by cell‐to‐cell contact in hepatitis C virus (HCV) transmission was studied in vitro by cell co‐cultivation experiments. A human lymphoblastoid B‐cell line, infected persistently with HCV in vitro (TO.FEHCV), was used as HCV donor [Serafino et al., 2003]; recipient cells were the human hepatoma HepG2 cell line. Both cell types were co‐cultured for 48 hr to allow the cell‐to‐cell contacts. The hepatoma HepG2 cells are not permissive to free‐virus infection, but they were infected successfully using TO.FEHCVcells as source of virus. The kinetics of viral RNA synthesis and the percentage of infected cells were compared in cell‐mediated‐and cell‐free‐viral infection. After co‐cultivation, a consistent proportion of hepatoma cells replicated HCV and stably expressed viral antigens. Virus produced was infectious as demonstrated by the ability to reinfect fresh B‐cells. This cell model shows that permissiveness to HCV infection can be achieved in vitro in non‐permissive hepatoma cells by direct cell‐to‐cell contacts with infected human B‐cells. This mechanism of virus spread may also play a pathogenic role in vivo. J. Med. Virol.78:192–201, 2006. © 2005 Wiley‐Liss, Inc.

Published
2006
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34. Virological Characterization of the First 2 COVID-19 Patients Diagnosed in Italy: Phylogenetic Analysis, Virus Shedding Profile From Different Body Sites, and Antibody Response Kinetics

Author

Colavita, Francesca, Lapa, Daniele, Carletti, Fabrizio, Lalle, Eleonora, Messina, Francesco, Rueca, Martina, Matusali, Giulia, Meschi, Silvia, Bordi, Licia, Marsella, Patrizia, Nicastri, Emanuele, Marchioni, Luisa, Mariano, Andrea, Scorzolini, Laura, Ascoli Bartoli, Tommaso, Di Caro, Antonino, Ippolito, Giuseppe, Capobianchi, Maria Rosaria, Castilletti, Concetta, Abbate, Isabella, Agrati, Chiara, Aleo, Loredana, Alonzi, Tonino, Amendola, Alessandra, Apollonio, Claudia, Arduini, Nicolina, Bartolini, Barbara, Berno, Giulia, Biancone, Silvia, Biava, Mirella, Bibbò, Angela, Brega, Carla, Canali, Marco, Cannas, Angela, Carrara, Stefania, Casetti, Rita, Chiappini, Roberta, Ciafrone, Lucia, Cimini, Eleonora, Coen, Sabrina, Condello, Rossella, Coppola, Antonio, D’Arezzo, Silvia, Di Filippo, Stefania, Di Giuli, Chiara, Fabeni, Lavinia, Felici, Luisa, Ferraioli, Valeria, Forbici, Federica, Garbuglia, Anna Rosa, Giombini, Emanuela, Gori, Caterina, Graziano, Silvia, Gruber, Cesare Ernesto Maria, Khouri, Daniele, Leone, Barbara, Massimino, Chiara, Mazzarelli, Antonio, Minosse, Claudia, Montaldo, Claudia, Neri, Stefania, Nisii, Carla, Petrivelli, Elisabetta, Petroni, Fabrizio, Petruccioli, Elisa, Pisciotta, Marina, Pizzi, Daniele, Prota, Gianluca, Raparelli, Fabrizio, Rozera, Gabriella, Sabatini, Rossella, Sarti, Silvia, Sberna, Giuseppe, Sciamanna, Roberta, Selleri, Marina, Selvaggi, Carla, Sias, Catia, Stellitano, Chiara, Toffoletti, Antonietta, Truffa, Silvia, Turchi, Federica, Valli, Maria Beatrice, Venditti, Carolina, Vescovo, Tiziana, Vincenti, Donatella, Vulcano, Antonella, Zambelli, Emma, Abbonizio, Maria Alessandra, Albarello, Fabrizio, Amadei, Gioia, Antonini, Mario, Barbaro, Raffaella, Benigni, Martina, Bevilacqua, Nazario, Bordoni, Veronica, Branca, Marta, Campioni, Paolo, Caporale, Cinzia, Caravella, Ilaria, Ciaralli, Carmine, Corpolongo, Angela, Cristofaro, Massimo, Curiale, Salvatore, D’Abramo, Alessandra, Dantimi, Cristina, De Angelis, Alessia, De Angelis, Giada, Di Lorenzo, Rachele, Di Stefano, Federica, Ferraro, Federica, Fiorentini, Lorena, Frustaci, Andrea, Gallì, Paola, Garotto, Gabriele, Giancola, Maria Letizia, Giansante, Filippo, Greci, Maria Cristina, Lanini, Simone, Lepore, Luciana, Lucia, Andrea, Lufrani, Franco, Macchione, Manuela, Marani, Alessandra, Marini, Maria Cristina, Maritti, Micaela, Montaldo, Chiara, Murachelli, Silvia, Noto, Roberto, Palazzolo, Claudia, Pallini, Emanuele, Passeri, Virgilio, Pelliccioni, Federico, Petrecchia, Antonella, Petrone, Ada, Petrosillo, Nicola, Pianura, Elisa, Pisciotta, Maria, Pittalis, Silvia, Proietti, Costanza, Puro, Vincenzo, Rinonapoli, Gabriele, Sacchi, Alessandra, Sanasi, Francesco, Santagata, Carmen, Scarcia, Silvana, Schininà, Vincenzo, Scognamiglio, Paola, Stazi, Giulia, Vaia, Francesco, and Vairo, Francesco

Subjects
0301 basic medicine, Immunoglobulin A, Immunoglobulin G, Virus, NO, Serology, 03 medical and health sciences, 0302 clinical medicine, Major Article, SARS-cov-2, Medicine, 030212 general & internal medicine, Viral shedding, Neutralizing antibody, Phylogenesis, biology, Viral culture, business.industry, Antibody response, COVID-19, Italy, Virus shedding, 3. Good health, AcademicSubjects/MED00290, 030104 developmental biology, Infectious Diseases, Oncology, Immunoglobulin M, Immunology, biology.protein, business
Abstract

Background The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection of viral RNA from different anatomical districts and the antibody profile in the first 2 COVID-19 cases diagnosed in Italy. Methods We tested for SARS-CoV-2 RNA clinical samples, either respiratory and nonrespiratory (ie, saliva, serum, urine, vomit, rectal, ocular, cutaneous, and cervico-vaginal swabs), longitudinally collected from both patients throughout the hospitalization. Serological analysis was carried out on serial serum samples to evaluate IgM, IgA, IgG, and neutralizing antibody levels. Results SARS-CoV-2 RNA was detected since the early phase of illness, lasting over 2 weeks in both upper and lower respiratory tract samples. Virus isolate was obtained from acute respiratory samples, while no infectious virus was rescued from late respiratory samples with low viral RNA load, collected when serum antibodies had been developed. Several other specimens came back positive, including saliva, vomit, rectal, cutaneous, cervico-vaginal, and ocular swabs. IgM, IgA, and IgG were detected within the first week of diagnosis, with IgG appearing earlier and at higher titers. Neutralizing antibodies developed during the second week, reaching high titers 32 days after diagnosis. Conclusions Our longitudinal analysis showed that SARS-CoV-2 RNA can be detected in different body samples, which may be associated with broad tropism and different spectra of clinical manifestations and modes of transmission. Profiling antibody response and neutralizing activity can assist in laboratory diagnosis and surveillance actions.

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35. Partial N-gene target failure in the Seegene Allplex SARS-CoV-2 Master Assay as a proxy of SARS-CoV-2 BA.2.86.

Author

Valli MB, Schiavone ML, Rueca M, Berno G, Spezia PG, Gruber CEM, Forbici F, Fabeni L, Focosi D, Girardi E, Meledandri M, and Maggi F

Subjects
Humans, Coronavirus Nucleocapsid Proteins genetics, COVID-19 Nucleic Acid Testing methods, Phosphoproteins genetics, RNA, Viral genetics, COVID-19 Testing methods, SARS-CoV-2 genetics, SARS-CoV-2 isolation & purification, COVID-19 virology, COVID-19 diagnosis
Abstract

Competing Interests: The authors declare no conflict of interest.

Published
2024
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