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By using long pulses with extended dwelling time, lowered pulse repetition frequency (PRF) and reduced Nyquist velocity, the radar measurement capability can be enhanced to increase clear-air Doppler velocity data coverage (which is beneficial for radar wind analysis and data assimilation and motivated our previous study). This potential capability is further explored (beyond our previous study) by not only modifying the existing operational clear-air scan mode with a lowered PRF and reduced the Nyquist velocity (to nearly 12 m/s) but also coupling this low-PRF scan with a high-PRF scan into a new dual-PRF scan mode. With this dual-PRF scan mode, the velocities from the high-PRF scan can be used to dealias the raw velocities from the low-PRF scan. Doppler velocities collected by using the low-PRF scan in this dual-PRF scan mode exhibit enhanced clear-air data coverage but encounter increased difficulties in dealiasing beyond the radial range covered by the high-PRF scan. To overcome the encountered difficulties, the previously developed alias-robust variational method for analyzing severely aliased radar velocity observations with small Nyquist velocities is further improved adaptively, so reliable reference radial velocities can be obtained and used to dealias the low-PRF scanned raw velocities on range circles beyond those covered by the high-PRF scan.

Introduction: COVID-19 pandemic affects human health and the global economy. Its evolution is unpredictable, making it hard for governments to provide response actions suited for all populations. Meanwhile, informal street workers carry on with their labor despite contingency measures to sustain their lives. The objective was to conduct a case-control study to become aware of how street vendors' economy is affected during the COVID-19 pandemic. Methodology: During phase 2 of the COVID-19 pandemic in a Mexican suburban city. We interviewed informal street vendors (cases) and formal employees (controls). Results: Before mobility restrictions were in place, population income came 1.5% from formal employment and 23.5% from informal employment (street vendors). Informal employees lived on less than the equivalent of 1.5 Big Macs per day (p

Developing new polymer composites with special properties for 3D printing is one of the most attractive tasks. In our study, we have developed new polymer powders based on rGO-coated polyamide suitable for Selective Laser Sintering (SLS) printing. The obtained materials were characterized using optical microscopy, UV-Vis spectroscopy, scanning electron microscopy and dielectric spectroscopy. The size distribution of the powder particles, the angle of rest after an avalanche, the compactibility (Hausner ratio), the optical properties (absorption and transmission) of the powder monolayer, and the electrical conductivity of the obtained materials have been studied. It has been shown that the developed powders are suitable for the use in the framework of the SLS method, and can be applied to remove static electricity, as well as in reflective screens for electromagnetic radiation.

High-sensitivity weather radars easily detect nonmeteorological phenomena characterized by weak radar returns. Fireworks are the example presented here. To understand radar observations, an experiment was conducted in which the National Severe Storms Laboratory (NSSL)’s research (3-cm wavelength) dual-polarization radar and a video camera were located at 1 km from fireworks in Norman, Oklahoma. The fireworks from the 4 July 2017 celebration were recorded by both instruments. The experiment is described. Few bursts recorded by the camera are analyzed to obtain the height of the explosion, its maximum diameter, number of stars, and the duration of the visible image. Radar volume scans are examined to characterize the height of the observation, the maximum reflectivity, and its distribution with height. The fireworks location is close to the Terminal Doppler Weather Radar (TDWR) that operates in single polarization at a 5-cm wavelength and monitors hazardous weather over the Oklahoma City airport. A third radar with data from the event is the Weather Surveillance Radar-1988 Doppler (WSR-88D) located in Norman. It has a wavelength of 10 cm and supports technical developments at the Radar Operation Center. Reflectivity factors measured by the three radars are compared to infer the size of dominant scatterers. The polarimetric characteristics of fireworks returns are analyzed. Although these differ from those of precipitation, they are indistinguishable from insect returns. Radar observation of larger fireworks in Fort Worth, Texas, with a WSR-88D is included and compared with the observations of the smaller fireworks in Norman. We expect the detectability of explosions would be similar as of fireworks. Pinpointing locations would be useful to first responders, or air quality forecasters. A benefit of fireworks recognition in weather radar data is that it can prevent contamination of precipitation accumulations.

Rheumatoid arthritis is globally present in about 1% of the population. This autoinflammatory disease modifies the connective tissue, causing pain and inflammation of the joints. Over time, it causes the loss of joint cartilage and bone mass, decreasing the patient's quality of life. Treatment options now available either give symptomatic alleviation or alter the disease process. Nonetheless, adherence to chronic treatment is typically limited due to adverse effects. As a result, new therapy approaches, such as systemic administration of neutral electrolyzed saline to improve patients’ quality of life, are being investigated. The study is a randomized prospective preclinical trial with a single-blind and a 4-arm parallel group using a collagen-induced mice model to generate rheumatoid arthritis. It was carried out on 36 male BALB/c mice, with the primary outcome measure being a scoring system for histopathologic assessment. When all groups are compared, there are significant differences. In addition, the animal model was validated by the healthy group. The animals treated with neutral electrolyzed saline had much less cartilage degradation, bone erosion, pannus development, and inflammation than the placebo-treated mice. Serum IL-6 levels were evaluated in parallel with disease severity expressed as synovitis grading of the affected joints. Spearman’s rank correlation coefficient (Rs) = 0.399 ( P = 0.016 ) between serum IL-6 levels and the synovitis grading suggests a direct correlation between IL-6 production and disease severity. An additional trial of 20 male BALB/c mice (10 treated with placebo and 10 with neutral electrolyzed saline for 30 days) showed no clinical nor histopathological evidence of adverse effects. According to histopathological and blood test results, we conclude that neutral electrolyzed saline minimizes mechanical and inflammatory damage to the joint and may be helpful as an alternative to rheumatoid arthritis therapy.

Mefenamic acid is a non‑steroidal anti‑inflammatory drug exhibiting a wide range of anti‑inflammatory, antipyretic, analgesic and probable antiviral activities. The present study evaluated the efficacy of treatment with mefenamic acid combined with standard medical care vs. standard medical care plus a placebo in ambulatory patients with coronavirus disease 2019 (COVID‑19; nasal/oropharyngeal swabs reverse transcription‑PCR test results positive for severe acute respiratory syndrome coronavirus 2). The present study is a phase II prospective, two‑arm, parallel‑group, randomized, double‑blind placebo‑controlled clinical trial which analyzed 36 patients. Two aspects were evaluated during the 14‑day follow‑up period: i) The time for reaching a patient acceptable symptom state (PASS), and ii) the last day of each COVID‑19 symptom presentation. Adverse effects were evaluated. The clinical severity for all the patients in the study was mild (88.9%) and moderate (11.1%). The control (placebo) group achieved PASS on day 8.0±1.3, compared with day 4.4±0.8 in the mefenamic acid group (P=0.020, Kaplan‑Meier analyses using log‑rank tests). Patients that received mefenamic acid plus standard medical care had a ~16‑fold higher probability of achieving PASS on day 8 (adjusted RR, 15.57; 95% CI, 1.22‑198.71; P=0.035), compared with the placebo plus standard medical care group. All symptoms lasted for fewer days in the mefenamic acid group, compared with the placebo group; however, only the symptoms of headache (P=0.008), retro‑orbital eye pain (P=0.049), and sore throat (P=0.029) exhibited statistically significant differences. The experimental treatment produced no severe adverse effects. On the whole, the present study demonstrates that the administration of mefenamic acid markedly reduced the symptomatology and time to reach PASS in ambulatory patients with COVID‑19. Due to its probable antiviral effects and potent anti‑inflammatory mechanisms, mefenamic acid may prove to be useful in the treatment of COVID‑19, in combination with other drugs, including the new antivirals (remdesivir, molnupiravir, or favipiravir). However, future studies are also required to confirm these findings.

NEXRAD radars detect biological scatterers in the atmosphere, i.e., birds and insects, without distinguishing between them. A method is proposed to discriminate these bird and insect echoes. Multiple scans are collected for mass migration of birds (insects) and coherently averaged along their different aspects to improve the data quality. Additional features are also computed to capture the dependence of bird (insect) echoes on the observed aspect, range, and local regions of space. Next, ridge classifier and decision tree machine learning algorithms are trained on the collected data. For each method, classifiers are trained, first with the averaged dual pol inputs and then different combinations of the remaining features are added. The performance of both methods, are analyzed using metrics computed on a held-out test data set. Further case studies on roosting birds, bird migration, and insect migration cases, are conducted to investigate the performance of the classifiers when applied to new scenarios. Overall, the ridge classifier using only dual polarization variables was found to perform consistently well on both the test data and in the case studies. This classifier is recommended for operational use on the US Next-Generation Radars (NEXRAD) in conjunction with the existing Hydrometeor Classification Algorithm (HCA). The HCA would be used first to separate biological from non-biological echoes, then the ridge classifier could be applied to categorize biological echoes into birds and insects. To the best of our knowledge, this study is the first to train a machine learning classifier that can detect diverse patterns of bird and insect echoes, based on dual polarization variables at each range gate.

Background: Coronavirus disease (COVID-19) is currently the main public health problem worldwide. The administration of neutral electrolyzed saline, a solution that contains reactive species of chlorine and oxygen (ROS), may be an effective therapeutic alternative due to its immunomodulating characteristics, in systemic inflammation control, as well as in immune response improvement, promoting control of the viral infection. The present study evaluated the efficacy of treatment with intravenous and/or nebulized neutral electrolyzed saline combined with usual medical care versus usual medical care alone, in ambulatory patients with COVID-19. Methods: A prospective, 2-arm, parallel group, randomized, open-label, phase I-II clinical trial included 39 patients in the control group (usual medical care alone) and 45 patients in the experimental group (usual medical care + intravenous and/or nebulized electrolyzed saline, with dose escalation). Two aspects were evaluated during the twenty-day follow-up: i) the number of patients with disease progression (hospitalization or death); and ii) the Patient Acceptable Symptom State (PASS), a single-question outcome that determines patient well-being thresholds for pain and function. Biochemical and hematologic parameters, as well as adverse effects, were evaluated in the experimental group. Results: The experimental treatment decreased the risk for hospitalization by 92% (adjusted RR=0.08, 95% CI: 0.01–0.50, P=0.007), with a 43-fold increase in the probability of achieving an acceptable symptom state on day 5 (adjusted RR= 42.96, 95% CI: 9.22–200.0, P

By using long pulses with extended dwell time, radar sensitivity is enhanced such that more clear-air returns are usable. This enhanced capability can increase clear-air data coverage and provide additional information for radar wind analysis and data assimilation. To explore this potential capability and related benefits, a new scan mode with long pulses and low antenna rotation rate is derived from the existing clear-air scan mode and applied to the named KOUN S-band test bed radar at NSSL. Velocities collected using this newly modified scan mode are significantly enhanced in data coverage compared to those collected with the unmodified scan mode from the nearby test bed radar (named KCRI). However, transmitter duty cycle limits the pulse repetition frequency (PRF) to 455 Hz for the long pulses, which reduces the Nyquist velocity to 12.53 m s−1 for the new scan mode. Such a small Nyquist interval leads to significantly worse problems with aliased velocities. A recently developed dealiasing algorithm is modified by using external reference velocities from KCRI to overcome increased difficulties in dealiasing, making the velocities from KOUN useful over increased range and area. This implies that similar increases in velocity data coverage can be achieved without using external reference velocities if the newly modified scan is paired with an unmodified scan into dual-PRF scans. This dual-PRF approach is proposed for future development.

Adenovirus 5 (Ad-5) infection is a common cause of acute respiratory infections and the main vector used in gene therapy. There are few studies on the relationship of Ad-5 to obesity. In the present study, we evaluated the chronic effects of Ad-5 infection on golden (Syrian) hamsters fed either a balanced diet (BD) or a high-fat diet (HFD). After a single inoculation with Ad-5 (1 × 107 pfu), the body weight of the animals was measured weekly. Medium-term (22 weeks) serum biochemical analyses and long-term (44 weeks) liver morphology, adiposity, and locomotive functionality (movement velocity) assessments were carried out. In the animals fed the BD, adenovirus infection produced hyperglycemia and hyperlipidemia. In the long term, it produced a 57% increase in epididymal pad fat and a 30% body weight gain compared with uninoculated animals. In addition, morphological changes related to non-alcoholic fatty liver disease (NAFLD) were observed. The animals fed the HFD had similar but more severe changes. In addition, the hamsters presented an obesity paradox: at the end of the study, the animals that had the most morphological and functional changes (significantly reduced movement velocity) had the lowest body weight. Despite the fact that an HFD appears to be a more harmful factor in the long term than adenovirus infection alone, infection could increase the severity of harmful effects in individuals with an HFD. Epidemiological studies are needed to evaluate the effect of adenovirus as a precursor of chronic liver and cardiovascular diseases, including the chronic effects of gene therapy.

Strong in-cloud electric fields align ice particles that can be observed with polarization diversity radars. Radar data collected in the simultaneous transmission mode, wherein horizontally and vertically polarized waves are simultaneously transmitted and received (SHV), and in a mode whereby a single-polarization wave is transmitted and dual (orthogonal)-polarization waves are received simultaneously [linear depolarization (LDR) mode] are analyzed. The necessary time delay between the SHV and LDR modes for our radar was about 1–4 min. The data show that the areas of canted crystals from the LDR mode are larger than those from the SHV mode, thereby indicating that the LDR mode is more sensitive to canted ice cloud particles than the SHV mode. The data also demonstrate that the differential phase and correlation coefficient in the LDR mode are indicative of canted cloud crystals and that these variables often are more sensitive to canted crystals than the linear depolarization ratio studied earlier. Rapidly scanning radars such as those with a phased array antenna could operate sequentially in the SHV and LDR modes and thus better detect cloud volumes characterized by enhanced electric fields.

Chagas disease (ChD) is a parasitosis caused by the protozoan Trypanosoma cruzi (Tc). It is endemic to almost all Latin American countries, including the southern United States. The acute form of C...

During phase 2 of the COVID-19 pandemic in a Mexican City, informal street vendors (cases) and formal employees (controls) were interviewed. A total of 82.6% of street vendors preferred to expose themselves to the coronavirus than to stop working, compared with 18.4% of formal employees (adjusted OR = 19.4, 95%CI: 4.6-81.7, p < 0.001). Street vendors had 7 times less fear of dying from coronavirus (adjusted OR = 0.14, 95% CI: 0.03-0.5, p = 0.005) and showed a 16-times greater lack of real concern for the increase in cases in their community than the formal employees (adjusted OR = 0.06, 95% CI: 0.01-0.3, p = 0.002). Street vendors were the group with the poorest adherence to household and work area containment measures that continued to be in contact with others. The corresponding authorities must plan specific strategies that allow street vendors to survive economically, while at the same time, protecting community health.

Introduction: Due to the coronavirus pandemic, identifying the infected individuals has become key to limiting its spread. Virus nucleic acid real-time RT-PCR testing has become the current standard diagnostic method but high demand could lead to shortages. Therefore, we propose a detection strategy using a one-step nested RT-PCR. Methodology: The nucleotide region in the ORF1ab gene that has the greatest differences between the human coronavirus and the bat coronavirus was selected. Primers were designed after that sequence. All diagnostic primers are species-specific since the 3´ end of the sequence differs from that of other species. A primer set also creates a synthetic positive control. Amplified products were seen in a 2.5% agarose gel, as well as in an SYBR Green-Based Real-Time RT-PCR. Results: Amplification was achieved for the positive control and specific regions in both techniques. Conclusions: This new technique is flexible and easy to implement. It does not require a real-time thermocycler and can be interpreted in agarose gels, as well as adapted to quantify the viral genome. It has the advantage that if the coronavirus mutates in one of the key amplification nucleotides, at least one pair can still amplify, thanks to the four diagnostic primers.

It is shown that the NEXRAD weather radar with enhanced detectability is capable of observing the evolution of convective thermals. The fields of radar differential reflectivity show that the upper parts of the thermals are observable due to Bragg scatter, whereas scattering from insects dominates in the lower parts. The thermal-top rise rate is between 1.5 and 3.7 m s−1 in the analyzed case. Radar observations of thermals also enable estimations of their maximum heights, horizontal sizes, and the turbulent dissipation rate within each thermal. These attributes characterize the intensity of convection.

The mean axis ratio (length/width) and the degree of orientation of cloud ice particles are retrieved from radar differential reflectivity (ZDR) and the copolar correlation coefficient (ρhv) measured with the S-band WSR-88D radar. Hardware differential phases and amplifications in the polarimetric channels affect measured ZDR and ρhv and are taken into consideration in the retrieval procedure. The retrieval is performed for particles in shapes of hexagonal prisms, which are closer to shapes of real cloud particles than frequently used spheroids. The median retrieved axis ratio for prisms is larger than that for spheroids. The statistical 1σ retrieval errors caused by fluctuations of radar returns are about 40% in areas of signal-to-noise ratios stronger than 10 dB. The values of the degree of orientation lie in an interval from 2° to 23°, which points to significant perturbations in the orientations of ice particles most likely caused by the wind field.

Clear-air Bragg scatter (CABS) is a refractivity gradient return generated by turbulent eddies that operational Weather Surveillance Radar-1988 Doppler (WSR-88D) systems can detect. The randomly oriented nature of the eddies results in a differential reflectivity (ZDR) value near 0 dB, and thus CABS can be used as an assessment of ZDR calibration in the absence of excessive contamination from precipitation or biota. An automated algorithm to estimate ZDR bias from CABS was developed by the Radar Operations Center and can be used to assess the calibration quality of the dual-polarized WSR-88D fleet. This technique supplements existing ZDR bias assessment tools, especially the use of other external targets, such as light rain and dry snow.The estimates of ZDR bias from CABS using a 1700–1900 UTC time window were compared to estimates from the light rain and dry snow methods. Output from the automated CABS algorithm had approximately the same amount of bias reported as the light rain and dry snow estimates (within ±0.1 dB). As the 1700–1900 UTC time window appeared too restrictive, a modified version of the algorithm was tested to detect CABS diurnally on a volume-by-volume basis (continuous monitoring). Continuous monitoring resulted in a two- to fourfold increase in the number of days with CABS detections. Results suggest estimates from CABS are viable for many sites throughout the year and provide an important addition to existing bias estimation techniques.

Studies have shown that echo returns from clear-air Bragg scatter (CABS) can be used to detect the height of the convective boundary layer and to assess the systematic differential reflectivity (ZDR) bias for a radar site. However, these studies did not use data from operational Weather Surveillance Radar-1988 Doppler (WSR-88D) or data from a large variety of sites. A new algorithm to automatically detect CABS from any operational WSR-88D with dual-polarization capability while excluding contamination from precipitation, biota, and ground clutter is presented here. Visual confirmation and tests related to the sounding parameters’ relative humidity slope, refractivity gradient, and gradient Richardson number are used to assess the algorithm. Results show that automated detection of CABS in operational WSR-88D data gives useful ZDR bias information while omitting the majority of contaminated cases. Such an algorithm holds potential for radar calibration efforts and Bragg scatter studies in general.

Background and objectives: Cardiac remodeling in pregnancy and postpartum is poorly understood. The aim of this study was to evaluate changes in cardiac fibrosis (pericardial, perivascular, and interstitial), as well as the expression of matrix metalloproteinases (MMP-1, MMP-2, and MMP-9) and their inhibitors (Tissue inhibitors of metalloproteinases, TIMP-1 and TIMP-4) during late pregnancy and postpartum in rat left ventricle. Materials and Methods: Female Sprague&ndash, Dawley rats were used for this study. Rats were divided three groups: non-pregnant, late pregnancy, and postpartum. The heart was weighed and cardiac fibrosis was studied by conventional histological procedures. The expression and transcript level of target proteins were evaluated using immunoblot techniques and quantitative PCR. Results: The experiments showed an increase of perivascular, pericardial, and interstitial fibrosis in heart during pregnancy and its reversion in postpartum. Moreover, in late pregnancy, MMP-1, MMP-2, and MMP-9 metalloproteinases were downregulated and TIMP-1 and TIMP-4 were upregulated in left ventricle. Conclusions: Our data suggest that the metalloproteinases system is involved in the cardiac extracellular matrix remodeling during pregnancy and its reversion in postpartum, this improves the knowledge of the adaptive cardiac remodeling in response to a blood volume overload present during pregnancy.

Centruroides tecomanus is a medically important scorpion of the state of Colima (Mexico). This communication reports the identification of venom components of this scorpion with biological activity over insects/crickets (Acheta domestica), crustaceans/fresh water shrimps (Cambarellus montezumae), and mammalians/mice (Mus musculus, strain CD1). It also describes the pharmacological effects on cell lines in culture (L5178Y cells, HeLa cells, HuTu cells and Jurkat E6-1 cells), as well as on several types of bacteria (see below). The soluble venom of this scorpion was fractionated by high-performance liquid chromatography (HPLC) and collected separately in twelve independent fractions collected over 60 min run (5 min time apart each other). The HPLC components of fraction VII were lethal to all three species used for assay. The IVth fraction had a toxic effect on freshwater shrimps. In this species, fractions VI, VII and VIII were all lethal. For crickets, fractions V and VI were toxic and fraction VII was lethal. In mouse, the lethal components were found in fraction VII, whereas fraction VIII was toxic, but not lethal, at the doses assayed. The molecular weight of peptides from the various group of fractions were identified by mass spectrometry determination. Components lethal to mice showed molecular weights from 7013 to 7487 Da. Two peptides were obtained in homogeneous form and shown to be lethal to the three species of animal used for assay. The soluble venom tested on L5178Y cell line survival was shown to be cytotoxic, at 10-100 μg/mL concentration, when compared to control murine splenocytes (p = 0.007). The soluble venom applied to Hela, Hutu and Jurkat cell lines did not show cytotoxic effects at these concentrations. On the contrary, it seems to have a proliferative effect. However the HPLC fractions I, III, VI and XII do have a cytotoxic effect on Jurkat E06-1 cells in culture at 200 μg/mL concentration. The antimicrobial activity of the venom fractions on Staphylococcus aureus (gram-positive), Escherichia coli, Pseudomonas aeruginosa y Salmonella spp (gram-negative) was measured, using the liquid inhibition growth system. The four strains of bacteria used were susceptible to fractions III and IV, affecting all four bacterial strains at concentrations below 5 μg/mL.

Weather surveillance radars routinely detect smoke of various origin. Of particular significance to the meteorological community are wildfires in forests and/or prairies. For example, one responsibility of the National Weather Service in the USA is to forecast fire outlooks as well as to monitor wildfire evolution. Polarimetric variables have enabled relatively easy recognitions of smoke plumes in data fields of weather radars. Presented here are the fields of these variables from smoke plumes caused by grass fire, brush fire, and forest fire. Histograms of polarimetric data from plumes contrast these cases. Most of the data are from the polarimetric Weather Surveillance Radar 1988 Doppler (WSR-88D aka NEXRAD, 10 cm wavelength), hence, the wavelength does not influence these comparisons. Nevertheless, in one case, simultaneous observations of a plume by the operational Terminal Doppler Weather Radar (TDWR, 5 cm wavelength) and a WSR-88D is used to infer backscattering characteristic and, hence, sizes of dominant contributors to the returns. To interpret these measurements, Computational Electromagnetics (CEM) tools are applied. For one wildfire from Oklahoma, radar and satellite (GOES-16, Geostationary Operational Environmental Satellite) images are analyzed. The case demonstrates a potential to forecast fire intensification caused by a very rapid cold front. Finally, we suggest a possible way to extract the smoke plume return from the class of nonmeteorological scatterers.

Background Cedecea lapagei bacterium was discovered in 1977 but was not known to be pathogenic to humans until 2006. In the medical literature there are very few clinical case reports of Cedecea lapagei; none have reported a catastrophic death secondary to a soft tissue hemorrhagic bullae infection. As well as soft tissue infection, rare cases of pneumonia, urinary tract infections, peritonitis, osteomyelitis, bacteremia, and sepsis have been documented with the majority having good outcomes. Here, we present the first case of a fatal outcome in a Cedecea lapagei soft tissue infection with multiple hemorrhagic bullae. Case presentation A 52-year-old Mexican man with antecedents of liver cirrhosis and treated hypertension was brought to our institution with clinical signs of sepsis and 16 to 18 hours of history of pain and edema in his right lower limb. During the course of the first day hospitalized in our institution, he developed several large serohematogenous bullae with ascending progression on his entire right lower limb. He subsequently developed multiple organ failure and septic shock with rapid deterioration, dying on the second day. Bullae fluid samples taken the first day undoubtedly isolated Cedecea lapagei within the second day using MicroScan WalkAway® 96 plus System as well Gram-negative bacteria in MacConkey and blood agar. Conclusions The isolation of Cedecea lapagei was an unexpected etiological finding that will enable physicians in the future to consider this bacterium as a probable cause of serohematogenous bullae infections. We do not exclude contamination although it has never been isolated in bullae fluid in the medical literature. Future encounters with this bacterium should not be taken lightly as it may have the potential to have fatal outcomes.

Leptin is a protein hormone that plays a key role in the regulation of energy balance and glucose homeostasis. Leptin and all leptin receptor isoforms are present in the carotid bodies, but its precise function in glucose regulation and metabolism is not yet known. The aim of this study was to determine whether exogenous leptin, microinjected into the commissural nucleus tractus solitarii (cNTS), preceding sodium cyanide (NaCN) injection into the circulatory isolated carotid sinus (ICS), in vivo, modifies hyperglycemic reflex (HR) and brain glucose retention (BGR). In anesthetized Wistar rats (sodium pentobarbital, i.p. 3.3 mg/100 g/saline, Pfizer, Mex), arterial and venous blood samples were collected from silastic catheters implanted in the abdominal aorta and jugular sinus. Exogenous leptin (50 ng/20 nL of aCSF) or leptin vehicle (20 nL of aCSF) microinjected (stereotaxically) into the cNTS 4 min before NaCN (5 μg/100 g/50 μL saline into ICS) (experimental 1 [E1] and control 1[C1] groups, respectively) significantly increased HR and BGR compared with their basal values, but the increase was bigger in the E1 group. When leptin or aCSF were injected into the cNTS before saline (E2 and C2 groups, respectively) glucose responses did not vary when compared with their basal levels. Leptin and its receptors in the cNTS cells probably contribute to their sensitization during hypoxia.

Leptin is a protein hormone that plays a key role in the regulation of energy balance and glucose homeostasis. Leptin and all leptin receptor isoforms are present in the carotid bodies, but its precise function in glucose regulation and metabolism is not yet known. The aim of this study was to determine whether exogenous leptin, microinjected into the commissural nucleus tractus solitarii (cNTS), preceding sodium cyanide (NaCN) injection into the circulatory isolated carotid sinus (ICS), in vivo, modifies hyperglycemic reflex (HR) and brain glucose retention (BGR). In anesthetized Wistar rats (sodium pentobarbital, i.p. 3.3 mg/100 g/saline, Pfizer, Mex), arterial and venous blood samples were collected from silastic catheters implanted in the abdominal aorta and jugular sinus. Exogenous leptin (50 ng/20 nL of aCSF) or leptin vehicle (20 nL of aCSF) microinjected (stereotaxically) into the cNTS 4 min before NaCN (5 μg/100 g/50 μL saline into ICS) (experimental 1 [E1] and control 1[C1] groups, respectively) significantly increased HR and BGR compared with their basal values, but the increase was bigger in the E1 group. When leptin or aCSF were injected into the cNTS before saline (E2 and C2 groups, respectively) glucose responses did not vary when compared with their basal levels. Leptin and its receptors in the cNTS cells probably contribute to their sensitization during hypoxia.

It is shown that the dual-polarization radar parameters can be used to estimate the vertical extent of inflow regions in thunderstorms. Atmospheric biota trapped by inflows allows identification of inflow regions. It is shown that weak echoes from biota can be observed at signal-to-noise ratios as low as −7 dB. The vertical extent of inflow reached 5.2 km in the analyzed tornadic thunderstorm and 6.7 km in a severe thunderstorm.

To increase the scanning rate of phased-array radar by a factor of two or more, the contiguous transmission of two or more pulses toward two or more directions and simultaneous reception of weather echoes from those directions is employed. However, if there are strong gradients of reflectivity, mainlobe-to-sidelobe coupled echoes contaminate wanted mainlobe-to-mainlobe (m–m) coupled echoes and limit the accurate analysis of weather. To retrieve m–m echoes, a technique is derived that restores wanted m–m voltages for antenna sidelobe levels as high as $-$ 30 dB and the difference in echo power of 100 dB for two simultaneously sampled beam directions. The technique is demonstrated using data collected with the WSR-88D radar and simulating PAR operations.

Radar echoes from insects, birds, and bats in the atmosphere exhibit both symmetry and asymmetry in polarimetric patterns. Symmetry refers to similar magnitudes of polarimetric variables at opposite azimuths, and asymmetry relegates to differences in these magnitudes. Asymmetry can be due to different species observed at different azimuths. It is shown in this study that when both polarized waves are transmitted simultaneously, asymmetric patterns can also be caused by insects of the same species that are oriented in the same direction. A model for scattering of simultaneously transmitted horizontally and vertically polarized radar waves by insects is developed. The model reproduces the main features of asymmetric patterns in differential reflectivity: the copolar correlation coefficient and the differential phase. The radar differential phase on transmit between horizontally and vertically polarized waves plays a critical role in the formations of the asymmetric patterns. The width-to-length ratios of insects’ bodies and their orientation angles are retrieved from matching the model output with radar data.

Dengue virus (DENV) is currently considered as one of the most important mosquito-borne viral pathogens affecting humans. Genetic variations in viruses are likely to be a condition for more effective evasion of the immune system and resulting in severe clinical consequences. The DENV‑1 NS5 gene was sequenced to establish whether during an epidemic burst there were genetic variations of the virus and whether any variant was associated (through a case‑control design) with severe clinical behavior. A total of 31 patients positive for DENV‑1 were enrolled. Among the nucleotide differences between the sequences, only two generated amino acid changes. The variants 124Met/166Ser (amino acid positions according to the report GenBank AJL35015.1), were associated with a severe clinical course of the disease. Via in silico tests, it was identified that the variations generate changes in the protein probably affecting the function of type‑1 interferon, either at the level of its receptor or by interfering with the Janus kinase‑signal transducer and activator of transcription signaling pathway.

Arthralgia is a potentially incapacitating condition and a persistent symptom in chronic or acute episodes of Chikungunya fever caused by infection with the Chikungunya virus (CHIKV). To the best of our knowledge, there are no reports on risk factors associated with the intensity of arthralgias in typical acute episodes of the disease. Although a number of studies have reported on risk factors associated with the development of the chronic stage of the disease, smoking habits have not been analyzed. Smoking is an interesting factor to consider since it is the main environmental risk factor for the development of rheumatoid arthritis (RA), a similar disease to CHIKV in many aspects. In the present study, 140 patients infected with CHIKV were assessed for risk factors associated with severe arthralgia intensity in the acute phase (pain of 9/10 on the visual analog scale of 0-10) and moderate to severe intensity (according to the Routine Assessment of Patient Index Data 3) 3.5 months after infection in patients that experienced the chronic phase of the disease. Women and smokers were 2- to 3-times more likely to experience severe pain in the acute and chronic stages. Likewise, the presence of severe arthralgia during the acute disease phase resulted in a 4-fold increased risk for entering the chronic phase. Smoking was a more important risk factor in males compared with females. Smoking resulted in a 20-fold increased risk for severe arthralgia during the acute phase in men, as well as a 10-fold increased risk for developing chronic disease with moderate-to-severe pain 3.5 months after the acute stage. The presence of rash, headache, muscular weakness or conjunctivitis in the acute phase, the presence of diabetes and age >40 years were considered significant risk factors due to their influence on illness progression. In conclusion, smoking and female sex were the main risk factors associated with development of severe joint pain in the acute and chronic phases of Chikungunya fever. These risk factors are similar to those associated with the development and severity of RA, possibly because the two diseases share pathophysiological mechanisms, including elevated interleukin-6 levels.

The present study was conducted to estimate the incidence of seropositivity to anti-Trypanosoma cruzi antibodies and analyze potential risk factors in Colima, on the western coast of Mexico.Longitudinal studies of 209 subjects with negative serology in 1999 for anti-Trypanosoma cruzi antibodies by hemagglutination inhibition test were tested again in 2005. At the same time, 716 children under six years of age were surveyed serologically (total n = 925); the history of Trypanosoma cruzi infection was determined by the same hemagglutination inhibition test. The variables analyzed were age, sex, living in triatomine-infested places, type of community, quality of housing, presence of pets, and number of inhabitants per house.Trypanosoma cruzi seropositivity in the period of six years was 22/925 cases, with a point prevalence of 2.73% and an adjusted rate of 7.3/1,000 person-years. The variable living in triatomine-infested areas showed association with seropositivity anti-Trypanosoma cruzi antibodies (RR: 5.5; 95% CI: 1.28-23.5). The remaining variables showed no significant association.This study confirms the active transmission of Chagas disease in Mexico´s western-central region, which merits greater epidemiological surveillance and vector control, particularly in localities infested with triatomines.

Lung hypoplasia, pulmonary persistent hypertension of the newborn and its morphological changes are the main features in congenital diaphragmatic hernia (CDH). This study was undertaken to investigate if antenatal use of sildenafil and/or bosentan attenuates vascular remodeling, promotes branching, and improves alveolarization in experimental nitrofeninduced CDH.Nitrofen (100 mg) was gavage-fed to pregnant rats at post conception day (PCD) 9 to induce CDH. The rats were randomized to 5 groups: 1) control; 2) nitrofen; 3) nitrofen+sildenafil 100 mg/kg per day at PCD 16-20; 4) nitrofen+bosentan 30 mg/kg per day, at PCD 16-20, and 5) nitrofen+bosentan+sildenafil, same doses and administration days. After cesarean delivery, the offsprings were sacrificed. The diaphragmatic defect and pulmonary hypoplasia were identified, and the lungs were dissected. Arterial wall thickness, bronchiolar density and alveolarization were assessed.The offsprings with CDH were characterized by severe pulmonary hypoplasia (lung weight-to-body weight ratio: 0.0263 [95% confidence interval (CI) 0.0242-0.0278)] in the nitrofen group versus 0.0385 (95% CI 0.0355-0.0424) in the control group (P=0.0001). Pulmonary arterial wall thickness was decreased to 3.0 (95% CI 2.8-3.7) μm in the nitrofen+sildenafil group versus 5.0 (95% CI 4.1-4.9) μm in the nitrofen group (P=0.02). Terminal bronchioles increased to 13.7 (95% CI 10.7-15.2) μm in the nitrofen+bosentan group in contrast to 8.7 (95% CI 7.2-9.4) μm in the nitrofen group (P=0.002). More significant differences (P=0.0001) were seen in terminal bronchioles in the nitrofen+sildenafil+bosentan group than in the nitrofen group [14.0 (95% CI 12.5-15.4) μm versus 8.5 (95% CI 7.1-9.3) μm]. Pulmonary arterial wall thickness was also decreased in the former group.In this rat model, antenatal treatment with sildenafil attenuates vascular remodeling. Bosentan promotes the development of terminal bronchioles in nitrofen-induced CDH.

Аннотация На современном этапе развития особую актуальность в науке приобретает синергетическая методология, определяющая практику моделирования саморазвивающихся систем, при этом пре-обладающей в исследовании становится интеграция научных подходов и синтез научных знаний, снимающие противоречия и образующие новое теоретическое единство. В статье представлены дефиниции понятий «фрактал», «резонанс», «родовой фрактал», «производный фрактал», «гло-бальная культура», раскрыта сущность фрактального и резонансного подходов и дано научное обоснование принципиально нового методологического подхода – фрактально-резонансного. Впервые авторы описывают взаимосвязь между синергетической, камертонной и эмоциональными культурами и обосновывают их закономерности с позиций постнеклассической научной парадигмы и фрактально-резонансного подхода. Особое внимание они уделяют рассмотрению базовых и до-полнительных компонентов синергетической, камертонной и эмоциональной культур, которые выступают в качестве их системообразующих единиц. Ключевые слова

The differential Doppler velocities (DDVs) measured with weather radar at horizontal and vertical polarizations in echoes from insects and birds are considered. In weather echoes, DDV is usually less than 0.5 ms- 1, whereas in echoes from flying birds and insects, it can reach 5-7 ms- 1. Such large difference can be used as an additional parameter in distinguishing between weather and nonmeteorological echoes. It is shown that large values of DDV pertain to multipeaked Doppler spectra with different spectral differential reflectivity values in the peaks.

The repeated injection of insulin (unconditioned stimulus, UCS) immediately followed by exposure to sensory stimulation (e.g. sound or odor; conditioned stimulus, CS) results in a learned conditioned reflex in which the exposure to the CS alone lowers blood glucose. The brain regions participating in this hypoglycemic Pavlovian response remain unknown. Here we investigate if nitric oxide (NO) in the nucleus tractus solitarius (NTS), a nucleus known to be involved in glucose homeostasis, participates in this hypoglycemic reflex. Insulin injections (UCS) were paired with exposure to menthol odor (CS). After 8-10 reinforcements (4-5days training), rats acquire the learned hypoglycemic response. An increase in c-Fos expression was observed in the NTS, the ventrolateral hypothalamic nucleus (VLH) and other brain regions of conditioned rats. Microinjections of 3-(5'-hydroxymethyl-2'furyl)-1-benzyl indazole (YC-1) a stimulator of soluble guanylate cyclase (sGC) into NTS before the UCS accelerated the acquisition of the learned hypoglycemic response; 5-6 reinforcement produced pronounced glucose drop when exposed to the CS. In contrast, an inhibitor of NO synthase (NOS) Nω-Nitro-l-arginine methyl ester (L-NAME) in the NTS prolonged the required training period (11-15 reinforcements) to obtain the hypoglycemic reflex, and reduced the glycemic response. The number of c-Fos expressing cells in the NTS and VLH in rats receiving YC-1was significantly higher than that observed in rats receiving L-NAME. These findings suggest that NO-cGMP-PKG signaling in the NTS can modify the acquisition of conditioned hypoglycemia, and suggests that this nucleus directly participates in this reflex.