Search

Your search keyword '"Valerio O"' showing total 89 results
Start Over Author "Valerio O"
89 results on '"Valerio O"'

2. Wolf and wild boar in the Alps: Trophic, temporal and spatial interactions in an Alpine protected area

Author

Valerio Orazi, Arianna Balacchi, Irene Belardi, Emanuela Rabajoli, Bruno Bassano, and Francesco Ferretti

Subjects
Spatiotemporal interactions, Predator – prey dynamics, Antipredator behavior, Alpine ecosystem, Recolonized areas, Ecology, QH540-549.5
Abstract

In predator-prey systems, interspecific interactions at the spatial and temporal scale may affect different components of the ecosystem. Despite a growing interest in the topic, little is known about these interactions in many regions, particularly in areas recently recolonized by apex predators, such as the Alps. Using a multidimensional approach, we assessed wolf-wild boar interactions in a recently recolonized protected area of the Western Alps (Gran Paradiso National Park, Italy) by combining camera trapping and wolf scat analyses. First, we quantified the importance of wild boar in the wolf diet by comparing two different periods (October 2018–September 2020 vs. October 2021–September 2022). Second, we evaluated prey selection in 2021–2022, when data on prey availability were collected. Then, we assessed spatiotemporal interactions between the wild boar and the wolf. Finally, we calculated combined spatiotemporal associations between the wolf and the wild boar in comparison to other ungulate prey. We found a remarkable increase in wild boar consumption between the two periods; roe deer and wild boar were consumed in proportion to their availability, while the chamois was positively selected and the red deer and the ibex were less consumed in relation to their availability. Although we found no negative association between wolf and wild boar detection rates, in winter the wild boar limited its temporal overlap with the predator by being less active in sites more frequently used by the wolf, suggesting some spatial modulation of a temporal antipredator response. Among ungulate species, the wild boar had the highest combined spatiotemporal association with the wolf, particularly in autumn-winter. Our results suggest complex interspecific interactions in alpine areas recently recolonized by the wolf.

Published
2024
Full Text
View/download PDF

9. IDH1 mutation is detectable in plasma cell-free DNA and is associated with survival outcome in glioma patients

Author

Stefania Crucitta, Francesco Pasqualetti, Alessandra Gonnelli, Martina Ruglioni, Giovanna Irene Luculli, Martina Cantarella, Valerio Ortenzi, Cristian Scatena, Fabiola Paiar, Antonio Giuseppe Naccarato, Romano Danesi, and Marzia Del Re

Subjects
Liquid biopsy, cfDNA, IDH1, Glioma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Circulating cell-free DNA (cfDNA, liquid biopsy) is a powerful tool to detect molecular alterations. However, depending on tumor characteristics, biology and anatomic localization, cfDNA detection and analysis may be challenging. Gliomas are enclosed into an anatomic sanctuary, which obstacles the release of cfDNA into the peripheral blood. Therefore, the advantages of using liquid biopsy for brain tumors is still to be confirmed. The present study evaluates the ability of liquid biopsy to detect IDH1 mutations and its correlation with survival and clinical characteristics of glioma patients. Methods Blood samples obtained from glioma patients were collected after surgery prior to the adjuvant therapy. cfDNA was extracted from plasma and IDH1 p.R132H mutation analysis was performed on a digital droplet PCR. χ2-test and Cohen k were used to assess the correlation between plasma and tissue IDH1 status, while Kaplan Meier curve and Cox regression analysis were applied to survival analysis. Statistical calculations were performed by MedCalc and GraphPad Prism software. Results A total of 67 samples were collected. A concordance between IDH1 status in tissue and in plasma was found (p = 0.0024), and the presence of the IDH1 mutation both in tissue (138.8 months vs 24.4, p

Published
2024
Full Text
View/download PDF

10. Strategies for polymer to polymer recycling fromwaste: Current trends and opportunities for improvingthe circular economy of polymers in South America

Author

Valerio, O., Muthuraj, R., and Codou, A.M.F.M.S.

Subjects
Polymer recycling, Industrial Innovation, Waste, South America, Strategies
Abstract

Polymers are one of the most versatile materials because oftheir excellent cost to performance ratio and durability. Thesereasons have driven the extensive usage of polymers in manycommon applications, and often, their lifespan exceeds theneeds of the application. This generates streams of polymerwastes which retain a substantial amount of their originalproperties and, therefore, could be recycled. Mechanical,chemical, and thermal recycling can be used for polymerrecycling, although in the latter case, the polymeric componentis lost by thermal degradation (energy recovery). This articlereviews the recycling strategies which allow producing newpolymeric materials from waste and the current researchtrends that could enable new opportunities for creating high-value polymeric products from what is now considered as waste.

Published
2020

11. AiroTouch: enhancing telerobotic assembly through naturalistic haptic feedback of tool vibrations

Author

Yijie Gong, Haliza Mat Husin, Ecda Erol, Valerio Ortenzi, and Katherine J. Kuchenbecker

Subjects
teleoperation, assembly tasks, vibrotactile feedback, audio equipment, user experience, Mechanical engineering and machinery, TJ1-1570, Electronic computers. Computer science, QA75.5-76.95
Abstract

Teleoperation allows workers to safely control powerful construction machines; however, its primary reliance on visual feedback limits the operator’s efficiency in situations with stiff contact or poor visibility, hindering its use for assembly of pre-fabricated building components. Reliable, economical, and easy-to-implement haptic feedback could fill this perception gap and facilitate the broader use of robots in construction and other application areas. Thus, we adapted widely available commercial audio equipment to create AiroTouch, a naturalistic haptic feedback system that measures the vibration experienced by each robot tool and enables the operator to feel a scaled version of this vibration in real time. Accurate haptic transmission was achieved by optimizing the positions of the system’s off-the-shelf accelerometers and voice-coil actuators. A study was conducted to evaluate how adding this naturalistic type of vibrotactile feedback affects the operator during telerobotic assembly. Thirty participants used a bimanual dexterous teleoperation system (Intuitive da Vinci Si) to build a small rigid structure under three randomly ordered haptic feedback conditions: no vibrations, one-axis vibrations, and summed three-axis vibrations. The results show that users took advantage of both tested versions of the naturalistic haptic feedback after gaining some experience with the task, causing significantly lower vibrations and forces in the second trial. Subjective responses indicate that haptic feedback increased the realism of the interaction and reduced the perceived task duration, task difficulty, and fatigue. As hypothesized, higher haptic feedback gains were chosen by users with larger hands and for the smaller sensed vibrations in the one-axis condition. These results elucidate important details for effective implementation of naturalistic vibrotactile feedback and demonstrate that our accessible audio-based approach could enhance user performance and experience during telerobotic assembly in construction and other application domains.

Published
2024
Full Text
View/download PDF

12. Odonate diversity of a highly urbanised region: An annotated checklist of the damselflies and dragonflies (Insecta, Odonata) of Lario and Brianza (Lombardy, N Italy)

Author

Gaia Bazzi, Andrea Galimberti, Claudio Foglini, Luciano Bani, Lionello Bazzi, Piero Bonvicini, Roberto Brembilla, Massimo Brigo, Alberto Cavenaghi, Giuseppe Colombo, Cesare Della Pietà, Carlo Galliani, Ettore Guarnaroli, Nicola Larroux, Alessandro Monti, Valerio Orioli, Francesco Ornaghi, Nicola Pilon, Giuliana Pirotta, Giovanni Radaelli, Giulia Tessa, and Giacomo Assandri

Subjects
Como, Lecco Monza and Brianza, Odonatofauna, urban, Biology (General), QH301-705.5
Abstract

Given their sensitivity to environmental alterations, odonates act as reliable bioindicators to assess the effects of changes in freshwater ecosystems and associated terrestrial habitats. The region comprised between Lario and Brianza (Provinces of Como, Lecco and Monza and Brianza - Lombardy, N Italy) is one of the most urbanised of the Italian peninsula and large parts of its territory have been heavily altered, especially at low elevation. Despite this pervasive anthropogenisation, the area is still characterised by a considerable variety of freshwater habitats, possibly harbouring rich odonate communities, which, however, have been never thoroughly investigated. This study aimed to produce the first commented checklist of the Odonata of this region, accompanied by distribution maps.The work is based on 12,093 records spanning from 1981 and 2022, derived from literature (289), revision of collections (42), citizen-science projects (1249) and unpublished data from the authors and their collaborators (10,513). Overall, fifty-five species occur - or occurred in the past - in the study area (20 Zygoptera and 35 Anisoptera). One species, Erythromma najas, was confirmed exclusively before 1978, while seven species (Lestes barbarus, Coenagrion scitulum, Aeshna affinis, Anax ephippiger, Somatochlora arctica, Sympetrum meridionale and Trithemis annulata) have been recorded only after 2000. Records referring to Chalcolestes parvidens and Sympetrum flaveolum were considered questionable and excluded from the checklist. A list of species for each protected site is additionally provided. This work highlighted the importance for odonates of Lario and Brianza Regions from a national perspective, in particular for species of conservation priority/interest, such as Sympecma paedisca, Oxygastra curtisii and Sympetrum depressiusculum.

Published
2023
Full Text
View/download PDF

15. Synaptic and transcriptomic features of cortical and amygdala pyramidal neurons predict inefficient fear extinction

Author

Daniela Laricchiuta, Juliette Gimenez, Giuseppe Sciamanna, Andrea Termine, Carlo Fabrizio, Francesco Della Valle, Silvia Caioli, Luana Saba, Marco De Bardi, Francesca Balsamo, Anna Panuccio, Noemi Passarello, Anna Mattioni, Elisa Bisicchia, Cristina Zona, Valerio Orlando, and Laura Petrosini

Subjects
CP: Neuroscience, CP: Cell biology, Biology (General), QH301-705.5
Abstract

Summary: Fear-related disorders arise from inefficient fear extinction and have immeasurable social and economic costs. Here, we characterize mouse phenotypes that spontaneously show fear-independent behavioral traits predicting adaptive or maladaptive fear extinction. We find that, already before fear conditioning, specific morphological, electrophysiological, and transcriptomic patterns of cortical and amygdala pyramidal neurons predispose to fear-related disorders. Finally, by using an optogenetic approach, we show the possibility to rescue inefficient fear extinction by activating infralimbic pyramidal neurons and to impair fear extinction by activating prelimbic pyramidal neurons.

Published
2023
Full Text
View/download PDF

16. Italian cancer figures, report 2014: Prevalence and cure of cancer in Italy

Author

Adamo, M., Alessi, D., Aletta, P., Amodio, R., Andreone, S., Angelin, T., Anghinoni, E., Annulli, M., Arciprete, C., Artioli, M., Autelitano, M., Baili, P., Balducci, C., Baracco, M., Baracco, S., Battisti, W., Bella, F., Bellatalla, C., Bellini, A., Belluardo, C., Benatti, P., Benedetto, G., Benfatto, L., Bernazza, E., Bianconi, F., Biavati, P., Bidoli, E., Birri, S., Bizzoco, S., Bonelli, L., Bonini, A., Borciani, E., Bordini, M., Bovo, E., Bozzani, F., Braghiroli, B., Brucculeri, M., Brunori, V., Bucalo, G., Bucchi, L., Bugliarello, E., Bulatko, A., Busco, S., Busso, P., Buzzoni, C., Calabrese, A., Calabretta, L., Caldarella, A., Candela, G., Cannone, G., Canu, L., Caparelli, M., Capocaccia, R., Cappelletti, M., Caprara, L., Carboni, D., Carletti, N., Caroli, S., Cascio, M., Cascone, G., Casella, C., Castaing, M., Cavalieri, D., L, Cecconami, L., Celesia, M., Cena, T., Cercato, M., Cesaraccio, R., Chiesa, R., Cirilli, C., Cocchioni, M., Codazzi, T., Cogno, R., Colamartini, A., Colanino, Z., A, Cometti, I., Contiero, P., Contrino, M., Corbinelli, A., Cordaro, C., Corti, M., Costa, A., Costarelli, D., Coviello, V., Crapanzano, G., Cremone, L., Crocetti, E., Cuccaro, F., Curatella, S., Cusimano, R., D Alò, D., Dal, C., Dal, T., Cin, A., Dal, M., Davini, C., Dottori, D., De, M., Angelis, R., Santis, D., De, E., Valiere, E., Dei, T., Demurtas, G., Devigili, E., Felice, D., Di, E., Grazia, L., Gregorio, D., Di, C., Norcia, R., Prima, D., Dinaro, Y., Distefano, R., Doa, N., Domati, F., Fabiano, S., Facchinelli, G., Falcini, F., Falk, M., Fanetti, A., Fattoruso, S., Federico, M., Ferrari, F., Ferrari, L., Ferretti, S., Fidelbo, M., Filipazzi, L., Fiore, A., Fiori, G., Foca, F., Forgiarini, O., Foschi, R., Francisci, S., Frasca, G., Frassoldi, E., Fusco, M., Gada, D., Garrone, E., Gasparotti, C., Gatta, G., Gatti, L., Gaudiano, C., Gennaro, V., Gentilini, M., Gerevini, C., Ghilardi, S., Ghisleni, S., Giacomin, A., Giavazzi, L., Gigli, A., Gilardi, F., Giorgetti, S., Giorgi, R., P, Giubelli, C., Giuliani, O., Giurdanella, M., Gola, G., Goldoni, C., Golizia, M., Greco, A., Guarda, L., Guttadauro, A., Guzzinati, S., Iachetta, F., Iannelli, A., Ieni, A., Intrieri, T., Kaleci, S., Rosa, L., F, Lando, C., Lavecchia, A., Lazzarato, F., Rose, L., Leone, A., Leone, R., Lonati, F., Lucchi, S., Luminari, S., Macci, L., Macerata, V., Madeddu, A., Maffei, S., Maghini, A., Magnani, C., Magnani, G., Magoni, M., Mallone, S., Mameli, G., Mancini, S., Mancuso, P., Mangone, L., Manneschi, G., Mannino, R., Mannino, S., Marani, E., Marchesi, C., Mariani, F., Martorana, C., Marzola, L., Maspero, S., Maule, M., Mazzei, A., Mazzoleni, G., Mazzucco, G., Melcarne, A., Merletti, F., Merlo, E., Michiara, M., Migliari, E., Minerba, S., Minicuzzi, A., Mizzi, M., Monetti, D., Morana, G., Moroni, E., Mosso, M., Muni, A., Mura, F., Natali, M., Negrino, L., Nemcova, L., Nicita, C., Ocello, C., Pala, F., Palumbo, M., Panciroli, E., Panico, M., Pannozzo, F., Pascucci, C., Pasolini, A., Pastore, G., Patriarca, S., Pedroni, M., Perrotta, C., Pesce, P., Petrinelli, A., Petrucci, C., Pezzarossi, A., Pezzuto, L., Piffer, S., Pinon, M., Antonio Pinto, Pintori, N., Pirani, M., Pirino, D., Pironi, V., Ponz, D., Leon, M., Prandi, R., Prazzoli, R., Puleio, M., Puppo, A., Quarta, F., Quattrocchi, M., Ramazzotti, V., Rashid, I., Ravaioli, A., Ravazzolo, B., Ravegnani, M., Reggiani Bonetti, L., Ricci, P., Rinaldi, E., Rizzello, R., Rognoni, M., Rollo, P., Roncaglia, F., Roncucci, L., Rosano, A., Rossi, F., Rossi, G., Rossi, M., Rossi, S., Rossini, S., Rosso, S., Rudisi, G., Ruggeri, M., Russo, A., Russo, M., Sacchettini, C., Sacchetto, L., Sacco, G., Sacerdote, C., Salvatore, S., Salvi, O., Sampietro, G., Santucci, C., Scheibel, M., Sciacca, S., Sciacchitano, C., Sciacchitano, S., Scuderi, T., Sechi, O., Seghini, P., Senatore, G., Serafini, G., Serraino, D., Sgargi, P., Sini, G., Sobrato, I., Soddu, M., Solimene, C., Spano, F., Spata, E., Sperduti, I., Spinosa, S., Staiti, R., Stocco, C., Stracci, F., Sunseri, R., Sutera, S., Tagliabue, G., Tamburo, L., Tamburrino, S., Taranto, V., Terracini, B., Tisano, F., Tittarelli, A., Tognazzo, S., Torrisi, A., Traina, A., Trama, A., Trapani, C., Tschugguel, B., Tumino, R., Usala, M., Vacirca, S., Valerio, O., Valla, K., Varvarà, M., Vasquez, E., Vassante, B., Vattiato, R., Vercelli, M., Vercellino, P., Vicentini, M., Villa, M., Virdone, S., Francesco Vitale, Vitale, M., Vitali, B., Vitali, M., Vitarelli, S., Zanchi, A., Zanetti, R., Zani, G., Zanier, L., Zappa, M., Zarcone, M., Zevola, A., Zorzi, M., Zucchetto, A., Zucchi, A., Adamo, MS, Alessi, D, Aletta, P, Amodio, R, Andreone, S, Angelin, T, Anghinoni, E, Annulli, ML, Arciprete, C, Artioli, ME, Autelitano, M, Baili, P, Balducci, C, Baracco, M, Baracco, S, Battisti, W, Bella, F, Bellatalla, C, Bellini, A, Belluardo, C, Benatti, P, Benedetto, G, Benfatto, L, Bernazza, E, Bianconi, F, Biavati, P, Bidoli, E, Birri, S, Bizzoco, S, Bonelli, L, Bonini, A, Borciani, E, Bordini, M, Bovo, E, Bozzani, F, Braghiroli, B, Brucculeri, MA, Brunori, V, Bucalo, G, Bucchi, L, Bugliarello, E, Bulatko, A, Busco, S, Busso, P, Buzzoni, C, Calabrese, A, Calabretta, L, Caldarella, A, Candela, G, Cannone, G, Canu, L, Caparelli, M, Capocaccia, R, Cappelletti, M, Caprara, L, Carboni, D, Carletti, N, Caroli, S, Cascio, MA, Cascone, G, Casella, C, Castaing, M, Cavalieri, d'Oro, L, Cecconami, L, Celesia, MV, Cena, T, Cercato, MC, Cesaraccio, R, Chiesa, R, Cirilli, C, Cocchioni, M, Codazzi, T, Cogno, R, Colamartini, A, Colanino, Ziino, A, Cometti, I, Contiero, P, Contrino, ML, Corbinelli, A, Cordaro, C, Corti, M, Costa, A, Costarelli, D, Coviello, V, Crapanzano, G, Cremone, L, Crocetti, E, Cuccaro, F, Curatella, S, Cusimano, R, D'Alò, D, Dal, Cappello, T, Dal, Cin, A, Dal, Maso, L, Davini, C, De, Dottori, M, De, Angelis, R, De, Santis, E, De, Valiere, E, Dei, Tos, AP, Demurtas, G, Devigili, E, Di, Felice, E, di, Grazia, L, Di, Gregorio, C, di, Norcia, R, Di, Prima, A, Dinaro, Y, Distefano, R, Doa, N, Domati, F, Fabiano, S, Facchinelli, G, Falcini, F, Falk, M, Fanetti, AC, Fattoruso, S, Federico, M, Ferrari, F, Ferrari, L, Ferretti, S, Fidelbo, M, Filipazzi, L, Fiore, AR, Fiori, G, Foca, F, Forgiarini, O, Foschi, R, Francisci, S, Frasca, G, Frassoldi, E, Fusco, M, Gada, D, Garrone, E, Gasparotti, C, Gatta, G, Gatti, L, Gaudiano, C, Gennaro, V, Gentilini, MA, Gerevini, C, Ghilardi, S, Ghisleni, S, Giacomin, A, Giavazzi, L, Gigli, A, Gilardi, F, Giorgetti, S, Giorgi, Rossi, P, Giubelli, C, Giuliani, O, Giurdanella, MC, Gola, G, Goldoni, CA, Golizia, MG, Greco, A, Guarda, L, Guttadauro, A, Guzzinati, S, Iachetta, F, Iannelli, A, Ieni, A, Intrieri, T, Kaleci, S, La, Rosa, F, Lando, C, Lavecchia, AM, Lazzarato, F, Le, Rose, L, Leone, A, Leone, R, Lonati, F, Lucchi, S, Luminari, S, Macci, L, Macerata, V, Madeddu, A, Maffei, S, Maghini, A, Magnani, C, Magnani, G, Magoni, M, Mallone, S, Mameli, G, Mancini, S, Mancuso, P, Mangone, L, Manneschi, G, Mannino, R, Mannino, S, Marani, E, Marchesi, C, Mariani, F, Martorana, C, Marzola, L, Maspero, S, Maule, M, Mazzei, A, Mazzoleni, G, Mazzucco, G, Melcarne, A, Merletti, F, Merlo, E, Michiara, M, Migliari, E, Minerba, S, Minicuzzi, A, Mizzi, M, Monetti, D, Morana, G, Moroni, E, Mosso, ML, Muni, A, Mura, F, Natali, M, Negrino, L, Nemcova, L, Nicita, C, Ocello, C, Pala, F, Palumbo, M, Panciroli, E, Panico, M, Pannozzo, F, Pascucci, C, Pasolini, A, Pastore, G, Patriarca, S, Pedroni, M, Perrotta, C, Pesce, P, Petrinelli, AM, Petrucci, C, Pezzarossi, A, Pezzuto, L, Piffer, S, Pinon, M, Pinto, A, Pintori, N, Pirani, M, Pirino, D, Pironi, V, Ponz, de, Leon, M, Prandi, R, Prazzoli, R, Puleio, M, Puppo, A, Quarta, F, Quattrocchi, M, Ramazzotti, V, Rashid, I, Ravaioli, A, Ravazzolo, B, Ravegnani, M, Reggiani-Bonetti, L, Ricci, P, Rinaldi, E, Rizzello, R, Rognoni, M, Rollo, PC, Roncaglia, F, Roncucci, L, Rosano, A, Rossi, F, Rossi, G, Rossi, M, Rossi, S, Rossini, S, Rosso, S, Rudisi, G, Ruggeri, MG, Russo, AG, Russo, M, Sacchettini, C, Sacchetto, L, Sacco, G, Sacerdote, C, Salvatore, S, Salvi, O, Sampietro, G, Santucci, C, Scheibel, M, Sciacca, S, Sciacchitano, C, Sciacchitano, S, Scuderi, T, Sechi, O, Seghini, P, Senatore, G, Serafini, G, Serraino, D, Sgargi, P, Sini, GM, Sobrato, I, Soddu, M, Solimene, C, Spano, F, Spata, E, Sperduti, I, Spinosa, S, Staiti, R, Stocco, C, Stracci, F, Sunseri, R, Sutera, Sardo, A, Tagliabue, G, Tamburo, L, Tamburrino, S, Taranto, V, Terracini, B, Tisano, F, Tittarelli, A, Tognazzo, S, Torrisi, A, Traina, A, Trama, A, Trapani, C, Tschugguel, B, Tumino, R, Usala, M, Vacirca, S, Valerio, O, Valla, K, Varvarà, M, Vasquez, E, Vassante, B, Vattiato, R, Vercelli, M, Vercellino, PC, Vicentini, M, Villa, M, Virdone, S, Vitale, F, Vitale, MF, Vitali, B, Vitali, ME, Vitarelli, S, Zanchi, A, Zanetti, R, Zani, G, Zanier, L, Zappa, M, Zarcone, M, Zevola, A, Zorzi, M, Zucchetto, A, and Zucchi, A

Subjects
Aged, 80 and over, Male, Medicine (all), Aged, Female, Humans, Italy, Middle Aged, Neoplasms, Prevalence, Registries, Survival Rate, Socio-culturale, Settore MED/42 - Igiene Generale E Applicata, 80 and over, cancer prevalence, cancer incidence, cancer in Italy
Abstract

This Report intends to estimate the total number of people still alive in 2010 after cancer diagnosis in Italy, regardless of the time since diagnosis, and to project these estimates to 2015. This study is also aimed to estimate the number of already cured cancer patients, whose mortality rates have become undistinguishable from that of the general population of the same age and sex.The study took advantage of the information from the AIRTUM database, which included 29 Cancer Registries (covering 21 million people, 35% of the Italian population). A total of 1,624,533 cancer cases diagnosed between 1976 and 2009 contributed to the study. For each registry, the observed prevalence was calculated. Prevalence for lengths of time exceeding the maximum duration of the registration and of the complete prevalence were derived by applying an estimated correction factor, the completeness index. This index was estimated by means of statistical regression models using cancer incidence and survival data available in registries with 18 years of observation or more. For 50 types or combinations of neoplasms, complete prevalence was estimated at 1.1.2010 as an absolute number and as a proportion per 100,000 inhabitants by sex, age group, area of residence, and years since diagnosis. Projections of complete prevalence for 1.1.2015 were computed under the assumption of a linear trend of the complete prevalence observed until 2010. Validated mixture cure models were used to estimate: the cure fraction, that is the proportion of patients who, starting from the time of diagnosis, are expected to reach the same mortality rate of the general population; the conditional relative survival (CRS), that is the cumulative probability of surviving some additional years, given that patients already survived a certain number of years; the time to cure, that is the number of years necessary so that conditional survival in the following five years (5-year CRS) exceeds the conventional threshold of 95% (i.e., mortality rates in cancer patients become undistinguishable compared to those of the general population); the proportion of patients already cured, i.e., people alive since a number of years exceeding time to cure.As of 1.1.2010, it was estimated that 2,587,347 people were alive after a cancer diagnosis, corresponding to 4.4% of the Italian population. A relevant geographical heterogeneity emerged, with a prevalence above 5% in northern registries and below 4% in southern areas. Men were 45% of the total (1,154,289) and women 55% (1,433,058). In the population aged 75 years or more, the proportions of prevalent cases were 20% in males and 13% in females, 11% between 60 and 74 years of age in both sexes. Nearly 600,000 Italian women were alive after a breast cancer diagnosis (41% of all women with this neoplasm), followed by women with cancers of the colon rectum (12%), corpus uteri (7%), and thyroid (6%). In men, 26% of prevalent cases (295,624) were patients with prostate cancer, 16% with either bladder or colon rectum cancer. The projections for 1.1.2015 are of three million (3,036,741) people alive after a cancer diagnosis, 4.9% of the Italian population; with a 20% increase for males and 15% for females, compared to 2010. The cure fractions were heterogeneous according to cancer type and age. Estimates obtained as the sum of cure fractions for all cancer types showed that more than 60% of patients diagnosed below the age of 45 years will reach the same mortality rate of the general population. This proportion decreased with increasing age and it was30% for cancer diagnosed after the age of 74 years. It was observed that 60% of all prevalent cases (1,543,531 people or 2.6% of overall Italian population) had been diagnosed5 years earlier (long-term survivors). Time to cure (5-year CRS95%) was reached in10 years by patients with cancers of the stomach, colon rectum, pancreas, corpus and cervix uteri, brain, and Hodgkin lymphoma. Mortality rates similar to the ones reported by the general population were reached after approximately 20 years for breast and prostate cancer patients. Five-year CRS remained95% for25 years after cancer diagnosis in patients with liver and larynx cancers, non-Hodgkin lymphoma, myeloma, and leukaemia. Time to cure was reached by 27% (20% in men and 33% in women) of all people living after a cancer diagnosis, defined as already cured.The study showed a steady increase over time (nearly +3% per year) of prevalent cases in Italy. A quarter of Italian cancer patients alive in 2010 can be considered as already cured. The AIRTUM Report 2014 describes characteristics of cancer patients and former-patients for 50 cancer types or combinations by sex and age. This detailed information promotes the conduction of studies aimed at expanding the current knowledge on the quality of life of these patients during and after the active phase of treatments (prevalence according to health status), on the long-term effects of treatments (in particular for paediatric patients), on the cost profile of cancer patients, and on rare tumours. All these observations have a high potential impact on health planning, clinical practice, and, most of all, patients' perspective.

Published
2014

17. ITALIAN CANCER FIGURES - REPORT 2015: The burden of rare cancers in Italy = I TUMORI IN ITALIA - RAPPORTO 2015: I tumori rari in Italia

Author

Busco, Susanna, Buzzoni, Carlotta, Mallone, Sandra, Trama, Annalisa, Castaing, Marine, Bella, Francesca, Amodio, Rosalba, Bizzoco, Sabrina, Cassetti, Tiziana, Cirilli, Claudia, Cusimano, Rosanna, De Angelis, Roberta, Fusco, Mario, Gatta, Gemma, Gennaro, Valerio, Giacomin, Adriano, Giorgi Rossi, Paolo, Mangone, Lucia, Mannino, Salvatore, Rossi, Silvia, Pierannunzio, Daniela, Tavilla, Andrea, Tognazzo, Sandro, Tumino, Rosario, Vicentini, Massimo, Vitale, Maria Francesca, Crocetti, Emanuele, Dal Maso, Luigino, Mazzoleni, G, Bulatko, A, Devigili, E, Tschugguel, B, De Valiere, E, Facchinelli, G, Falk, M, Vittadello, F, Coviello, V, Cuccaro, F, Calabrese, A, Pinto, A, Cannone, G, Vitali, Me, Sampietro, G, Ghisleni, S, Giavazzi, L, Zanchi, A, Zucchi, A, Giacomin, A, Vercellino, Pc, Andreone, S, Fedele, M, Barale, A, Germinetti, F, Magoni, M, Zani, G, Salvi, O, Puleio, M, Gerevini, C, Adorni, A, Chiesa, R, Lonati, F, Tumino, R, Cascone, G, Frasca, G, Giurdanella, Mc, Martorana, C, Morana, G, Nicita, C, Rollo, Pc, Ruggeri, Mg, Spata, E, Vacirca, S, Sciacca, S, Sciacchitano, C, Fidelbo, M, Paderni, F, Benedetto, G, Vasquez, E, Bella, F, Calabretta, L, Castaing, M, Di Prima, A, Ieni, A, Leone, A, Pesce, P, Sciacchitano, S, Torrisi, A, Varvarà, M, Sutera Sardo, A, Mazzei, A, Lavecchia, Am, Mancuso, P, Nocera, V, Gola, G, Corti, M, Grandi, L, Caparelli, M, Mannino, S, Belluardo, C, Bizzoco, S, Davini, C, Lucchi, S, Villa, M, Anghinoni, E, Di Norcia, R, Ferretti, S, Biavati, P, Marzola, L, Migliari, E, Carletti, N, Petrucci, C, Brosio, F, Piccinni, L, Valente, N, Barchielli, A, Buzzoni, C, Caldarella, A, Corbinelli, A, Di Dia PP, Intrieri, T, Manneschi, G, Nemcova, L, Susini, N, Visoli, C, Zappa, M, Serraino, D, Angelin, T, Bidoli, E, Birri, S, Dal Maso, L, De Dottori, M, De Santis, E, Forgiarini, O, Zucchetto, A, Zanier, L, Filiberti, Ra, Casella, C, Marani, E, Puppo, A, Celesia, Mv, Cogno, R, Garrone, E, Pannozzo, F, Busco, S, Rashid, I, Ramazzotti, V, Cercato, Mc, Natali, M, Battisti, W, Sperduti, I, Macci, L, Bugliarello, E, Bernazza, E, Tamburo, L, Rossi, M, Curatella, S, Tamburrino, S, Fattoruso, S, Valerio, O, Melcarne, A, Quarta, F, Golizia, Mg, Raho, Am, De Maria, V, Vitarelli, S, Ricci, P, Guarda, L, Gatti, L, Pironi, V, Pasolini, A, Bordini, M, Autelitano, M, Ghilardi, S, Leone, R, Filipazzi, L, Bonini, A, Giubelli, C, Russo, Ag, Quattrocchi, M, Distefano, R, Panciroli, E, Bellini, A, Pinon, M, Spinosa, S, Spagnoli, G, Goldoni, Ca, Valla, K, Braghiroli, B, Cirilli, C, Donini, M, Amendola, V, Cavalieri d'Oro, L, Rognoni, M, Le Rose, L, Merlo, E, Negrino, L, Pezzuto, L, Fusco, M, Bellatalla, C, Panico, M, Perrotta, C, Vassante, B, Vitale, Mf, Usala, M, Pala, F, Sini, Gm, Pintori, N, Canu, L, Demurtas, G, Doa, N, Vitale, F, Cusimano, R, Traina, A, Guttadauro, A, Cascio, Ma, Mannino, R, Ravazzolo, B, Brucculeri, Ma, Rudisi, G, Adamo, Ms, Amodio, R, Costa, A, Zarcone, M, Sunseri, R, Bucalo, G, Trapani, C, Staiti, R, Michiara, M, Bozzani, F, Sgargi, P, Borciani, E, Seghini, P, Faccini, F, Prazzoli, R, Mangone, L, Di Felice, E, Pezzarossi, A, Caroli, S, Sacchettini, C, Ferrari, F, Roncaglia, F, Vicentini, M, Falcini, F, Colamartini, A, Bucchi, L, Balducci, C, Ravegnani, M, Vitali, B, Cordaro, C, Caprara, L, Giuliani, O, Giorgetti, S, Palumbo, M, Vattiato, R, Ravaioli, A, Mancini, S, Caiazzo, Al, Cavallo, R, Colavolpe, Af, D'Alessandro, A, Iannelli, A, Lombardo, C, Senatore, G, Sensi, F, Cesaraccio, R, Sechi, O, Pirino, D, Mura, F, Contrino, Ml, Madeddu, A, Tisano, F, Muni, A, Dinaro, Y, Mizzi, M, Sacco, G, Aletta, P, Ziino, Ac, Maspero, S, Fanetti, Ac, Moroni, E, Cometti, I, Annulli, Ml, Cecconami, L, Minerba, S, Minicuzzi, A, Zanetti, R, Rosso, S, Patriarca, S, Prandi, R, Sobrato, I, Gilardi, F, Busso, P, Sacchetto, L, Candela, G, Scuderi, T, Crapanzano, G, Taranto, V, Piffer, S, Gentilini, Ma, Rizzello, R, Cappelletti, M, Stracci, F, D'Alò, D, Scheibel, M, Costarelli, D, Spano, F, Rossini, S, Santucci, C, Petrinelli, Am, Solimene, C, Bianconi, F, Brunori, V, Tagliabue, G, Contiero, P, Tittarelli, A, Fabiano, S, Maghini, A, Codazzi, T, Frassoldi, E, Gada, D, di Grazia, L, Ruzza, Mr, Dei Tos AP, Baracco, M, Baracco, S, Bovo, E, Dal Cin, A, Fiore, Ar, Greco, A, Guzzinati, S, Monetti, D, Rosano, A, Stocco, C, Tognazzo, S, Zorzi, M, Merletti, F, Magnani, C, Pastore, G, Terracini, B, Alessi, D, Cena, T, Lazzarato, F, Macerata, V, Maule, M, Mosso, Ml, Sacerdote, C, Cocchioni, M, Pascucci, C, Ponz de Leon, M, Domati, F, Rossi, G, Kaleci, S, Rossi, F, Benatti, P, Roncucci, L, Di Gregorio, C, Magnani, G, Pedroni, M, Maffei, S, Mariani, F, Reggiani-Bonetti, L, Gennaro, V, Benfatto, L, Lando, C, Mazzucco, G, Romanelli, A, Storchi, C, Sala, O, Gabbi, C, and Buzzoni, C.

Published
2016

18. Liver and muscle circadian clocks cooperate to support glucose tolerance in mice

Author

Jacob G. Smith, Kevin B. Koronowski, Thomas Mortimer, Tomoki Sato, Carolina M. Greco, Paul Petrus, Amandine Verlande, Siwei Chen, Muntaha Samad, Ekaterina Deyneka, Lavina Mathur, Ronnie Blazev, Jeffrey Molendijk, Arun Kumar, Oleg Deryagin, Mireia Vaca-Dempere, Valentina Sica, Peng Liu, Valerio Orlando, Benjamin L. Parker, Pierre Baldi, Patrick-Simon Welz, Cholsoon Jang, Selma Masri, Salvador Aznar Benitah, Pura Muñoz-Cánoves, and Paolo Sassone-Corsi

Subjects
CP: Metabolism, Biology (General), QH301-705.5
Abstract

Summary: Physiology is regulated by interconnected cell and tissue circadian clocks. Disruption of the rhythms generated by the concerted activity of these clocks is associated with metabolic disease. Here we tested the interactions between clocks in two critical components of organismal metabolism, liver and skeletal muscle, by rescuing clock function either in each organ separately or in both organs simultaneously in otherwise clock-less mice. Experiments showed that individual clocks are partially sufficient for tissue glucose metabolism, yet the connections between both tissue clocks coupled to daily feeding rhythms support systemic glucose tolerance. This synergy relies in part on local transcriptional control of the glucose machinery, feeding-responsive signals such as insulin, and metabolic cycles that connect the muscle and liver. We posit that spatiotemporal mechanisms of muscle and liver play an essential role in the maintenance of systemic glucose homeostasis and that disrupting this diurnal coordination can contribute to metabolic disease.

Published
2023
Full Text
View/download PDF

19. Canagliflozin on top of dual renin-angiotensin system blockade in a woman with partial acquired lipodystrophy, type 2 diabetes and severely proteinuric chronic kidney disease: a case report

Author

Edoardo Biancalana, Giovanni Ceccarini, Silvia Magno, Valerio Ortenzi, Domenico Giannese, Ferruccio Santini, and Anna Solini

Subjects
dual renin-angiotensin system blockade, proteinuria, canagliflozin, lipodystrophy, metreleptin, case report, Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract

Sodium glucose cotransporter 2 inhibitors have proven strong efficacy in reducing end-stage renal disease in patients with type 2 diabetes. We are presenting here the case of a 40-year-old woman with acquired partial lipodystrophy, type 2 diabetes and essential hypertension complicated by chronic kidney disease and proteinuria in the nephrotic range. She first came to our attention in 2012; estimated glomerular filtration rate (eGFR) was 41.5 ml/min/1.73 m2 and total proteinuria was 375 mg/24h; she was treated with dual renin angiotensin system blocking. Proteinuria significantly increased during the following years, reaching a nephrotic range (>5 g/day). A kidney biopsy revealed a tubule-interstitial involvement compatible with type 2 diabetes. Leptin replacement therapy, started in 2018, improved glycaemic control and lipid profile, also determining a reduction in insulin total daily dose. In 2019, after the publication of the CREDENCE study, canagliflozin was started on top of losartan and ramipril. After an initial, expected eGFR drop, kidney function stabilized, and albuminuria significantly reduced (from 4120 to 984 mg/24h), while serum potassium showed only minimal increase. At last follow-up (2022) total proteinuria was still reducing (510 mg/24h), while kidney function was substantially unchanged (eGFR 40 ml/min/1.73 m2). This case report suggests that, despite not recommended in international guidelines, the use of SGLT2i in combination with dual renin angiotensin system blockade should be considered in specific conditions and under close clinical monitoring.

Published
2023
Full Text
View/download PDF

20. Discovery of Molecular Markers to Discriminate Corneal Endothelial Cells in the Human Body

Author

Masahito, Y., Hiroko, O., Susumu, H., Satoshi, K., Alistair R, R.F., Hideya, K., Michael, R., J Kenneth Baillie, Michiel J, L.D.H., Vanja, H., Timo, L., Ivan, V.K., Marina, L., Masayoshi, I., Robin, A., Christopher, J.M., Terrence, F.M., Sebastian, S., Nicolas, B., Mette, J., Emmanuel, D., Erik, A., Christian, S., Ulf, S., Yulia, A.M., Charles, P., Morana, V., Jessica, S., Colin, A.S., Yuri, I., Margherita, F., Intikhab, A., Davide, A., Gabriel, M.A., John A, C.A., Peter, A., Magda, B., Sarah, B., Piotr, J.B., Anthony, G.B., Swati, P., Judith, A.B., Antje, B., Bodega, B., Alessandro, B., James, B., Frank, B., A Maxwell Burroughs, Andrea, C., Carlo, V.C., Daniel, C., Yun, C., Marco, C., Yari, C., Hans, C.C., Emiliano, D., Carrie, A.D., Michael, D., Alexander, D.D., Taeko, D., Finn, D., Albert S, B.E., Matthias, E., Karl, E., Mitsuhiro, E., Hideki, E., Michela, F., Lynsey, F., Hai, F., Mary, C.F., Geoffrey, J.F., Alexander, V.F., Malcolm, E.F., Martin, C.F., Rie, F., Shiro, F., Cesare, F., Masaaki, F., Jun-ichi, F., Teunis, B.G., Andrew, G., Thomas, G., Daniel, G., Julian, G., Sven, G., Reto, G., Stefano, G., Thomas, J.H., Masahide, H., Mitsuko, H., Matthias, H., Jayson, H., Akira, H., Yuki, H., Takehiro, H., Meenhard, H., Kelly, J.H., Shannan, J.H.S., Oliver, M.H., Ilka, H., Fumi, H., Lukasz, H., Kei, I., Tomokatsu, I., Boris, R.J., Hui, J., Anagha, J., Giuseppe, J., Bogumil, K., Chieko, K., Kaoru, K., Kaiho, A., Kazuhiro, K., Mutsumi, K., Artem, S.K., Takeya, K., Shintaro, K., Sachi, K., Shuji, K., Hiroshi, K., Yuki, I.K., Tsugumi, K., Judith, S.K., Tony, J.K., Juha, K., Levon, M.K., Toshio, K., S Peter Klinken, Alan, J.K., Miki, K., Soichi, K., Naoto, K., Haruhiko, K., Shigeo, K., Sarah, K., Atsutaka, K., Andrew, T.K., Jeroen F, J.L., Weonju, L., Andreas, L., Kang, L., Berit, L., Leonard, L., Alan, M., Ri-ichiroh, M., Jessica, C.M., Benoit, M., Anthony, M., Niklas, M., Alison, M., Yosuke, M., David, A.D.L.M., Hiromasa, M., Mitsuru, M., Kazuyo, M., Efthymios, M., Hozumi, M., Christine, L.M., Mitsuyoshi, M., Sayaka, N., Yutaka, N., Fumio, N., Toshiyuki, N., Yukio, N., Kenichi, N., Erik van Nimwegen, Noriko, N., Hiromi, N., Shohei, N., Tadasuke, N., Soichi, O., Naganari, O., Hiroshi, O., Mitsuhiro, O., Mariko, O., Yasushi, O., Valerio, O., Dmitry, A.O., Arnab, P., Robert, P., Margaret, P., Helena, P., Silvano, P., James G, D.P., Owen J, L.R., Jordan, A.R., Mamoon, R., Timothy, R., Patrizia, R., Marco, R., Sugata, R., Morten, B.R., Eri, S., Antti, S., Akiko, S., Shimon, S., Mizuho, S., Hiroki, S., Hironori, S., Suzana, S., Alka, S., Claudio, S., Erik, A.S., Gundula, G.S., Anita, S., Thierry, S., Guojun, S., Hisashi, S., Yishai, S., Jay, W.S., Christophe, S., Daisuke, S., Takaaki, S., Masanori, S., Rolf, K.S., Peter A, C.'.H., Michihira, T., Naoko, T., Jun, T., Hiroshi, T., Hideki, T., Zuotian, T., Mark, T., Hiroo, T., Tetsuro, T., Eivind, V., Marc van de Wetering, Linda, M.V.D.B., Roberto, V., Dipti, V., Ilya, E.V., Wyeth, W.W., Shoko, W., Christine, A.W., Louise, N.W., Ernst, W., Emily, J.W., Yoko, Y., Masayuki, Y., Misako, Y., Yohei, Y., Shigehiro, Y., Suzan, E.Z., Peter, G.Z., Xiaobei, Z., Silvia, Z., Kim, M.S., Harukazu, S., Carsten, O.D., Jun, K., Peter, H., Winston, H., Tom, C.F., Boris, L., Vladimir, B.B., Martin, S.T., Vsevolod, J.M., Albin, S., David, A.H., Piero, C., Yoshihide Hayashizaki Yoshihide, H., Motokazu, T., Kohji, N., Hubrecht Institute for Developmental Biology and Stem Cell Research, Amsterdam institute for Infection and Immunity, Infectious diseases, and Experimental Immunology

Subjects
Cell type, Corneal endothelium, Pathology, medicine.medical_specialty, medicine.medical_treatment, lcsh:Medicine, Settore BIO/11 - Biologia Molecolare, Nerve Tissue Proteins, Context (language use), Biology, Zona Pellucida Glycoproteins, Receptors, G-Protein-Coupled, Cornea, Corneal Transplantation, medicine, Humans, Regeneration, Endothelial dysfunction, lcsh:Science, Corneal transplantation, Membrane Glycoproteins, Multidisciplinary, Tissue Engineering, Regeneration (biology), lcsh:R, Egg Proteins, Endothelium, Corneal, Endothelial Cells, Membrane Proteins, Correction, Middle Aged, medicine.disease, medicine.anatomical_structure, Receptor, Serotonin, 5-HT1D, Bullous keratopathy, cardiovascular system, lcsh:Q, Carrier Proteins, Biomarkers, Research Article
Abstract

The corneal endothelium is a monolayer of hexagonal corneal endothelial cells (CECs) on the inner surface of the cornea. CECs are critical in maintaining corneal transparency through their barrier and pump functions. CECs in vivo have a limited capacity in proliferation, and loss of a significant number of CECs results in corneal edema called bullous keratopathy which can lead to severe visual loss. Corneal transplantation is the most effective method to treat corneal endothelial dysfunction, where it suffers from donor shortage. Therefore, regeneration of CECs from other cell types attracts increasing interests, and specific markers of CECs are crucial to identify actual CECs. However, the currently used markers are far from satisfactory because of their non-specific expression in other cell types. Here, we explored molecular markers to discriminate CECs from other cell types in the human body by integrating the published RNA-seq data of CECs and the FANTOM5 atlas representing diverse range of cell types based on expression patterns. We identified five genes, CLRN1, MRGPRX3, HTR1D, GRIP1 and ZP4 as novel markers of CECs, and the specificities of these genes were successfully confirmed by independent experiments at both the RNA and protein levels. Notably none of them have been documented in the context of CEC function. These markers could be useful for the purification of actual CECs, and also available for the evaluation of the products derived from other cell types. Our results demonstrate an effective approach to identify molecular markers for CECs and open the door for the regeneration of CECs in vitro.

Published
2015

21. Increased erosion of high-elevation land during late Cenozoic: evidence from detrital thermochronology off-shore Greenland

Author

Valerio Olivetti, Silvia Cattò, and Massimiliano Zattin

Subjects
Medicine, Science
Abstract

Abstract Mountain regions at high altitudes show deeply incised glacial valleys that coexist with a high-standing low-relief landscape, whose origin is largely debated. Whether the plateaus contributed to sediment production during the late Cenozoic is a currently debated issue in glacial geomorphology and paleoclimatology. In this study, we used detrital apatite fission-track dating of marine sediments to trace provenance and spatial variation in focused erosion over the last 7 million years. The decomposition of age distributions into populations reveals that, moving upwards through the sections, two young populations get younger, while two older populations get progressively older. We interpreted these trends as the effect of glacial erosion on the valley floors and an increased sediment contribution from the high elevations. To test this hypothesis, we compared the measured ages with synthetic age distributions, which represented a change in the elevation of focused erosion. We conclude that the central-eastern Greenland region is the main source of sediments, and in addition to enhanced valley incision, sediments have also been sourced from progressively higher elevations since 7 Ma. The ageing trend provides an unusual case in detrital thermochronology and a strong evidence that intensified Quaternary glaciations amplify the erosional process both in valley bottoms and at high elevations.

Published
2022
Full Text
View/download PDF

22. New Evidence on the Linkage of Population Trends and Species Traits to Long-Term Niche Changes

Author

Pietro Tirozzi, Valerio Orioli, Olivia Dondina, and Luciano Bani

Subjects
breeding birds, migration strategy, farmland birds, niche conservatism, niche divergence, species specialization, Ecology, QH540-549.5, Animal culture, SF1-1100
Abstract

Despite the assessment of long-term niche dynamics could provide crucial information for investigating species responses to environmental changes, it is a poorly investigated topic in ecology. Here, we present a case study of multi-species niche analysis for 71 common breeding birds in Northern Italy, exploring long-term niche changes from 1992 to 2017 and their relationship with both population trends and species traits. We (i) quantified the realized Grinnellian niche in the environmental space, (ii) compared variations in niche breadth and centroid, (iii) tested niche divergence and conservatism through equivalency and similarity tests, (iv) calculated niche temporal overlap, expansion and unfilling indices, and (v) investigated their association with both population changes and species traits. Results supported niche divergence (equivalency test) for 32% of species, although two-thirds were not supported by the similarity test. We detected a general tendency to adjust the niche centroids towards warmer thermal conditions. Increasing populations were positively correlated with niche expansion, while negatively correlated with niche overlap, albeit at the limit of the significance threshold. We found moderate evidence for a non-random association between niche changes and species traits, especially for body size, clutch size, number of broods per year, inhabited landscape type, and migration strategy. We encourage studies correlating long-term population trends and niche changes with species traits’ information and a specific focus on cause-effect relationship at both the single and multiple-species level.

Published
2022
Full Text
View/download PDF

23. Behind the scenes: How RNA orchestrates the epigenetic regulation of gene expression

Author

Arianna Mangiavacchi, Gabriele Morelli, and Valerio Orlando

Subjects
RNA, non coding RNA (ncRNA), epigenetics, gene expression, chromatin, Biology (General), QH301-705.5
Abstract

Non-coding DNA accounts for approximately 98.5% of the human genome. Once labeled as “junk DNA”, this portion of the genome has undergone a progressive re-evaluation and it is now clear that some of its transcriptional products, belonging to the non-coding RNAs (ncRNAs), are key players in cell regulatory networks. A growing body of evidence demonstrates the crucial impact of regulatory ncRNAs on mammalian gene expression. Here, we focus on the defined relationship between chromatin-interacting RNAs, particularly long non-coding RNA (lncRNA), enhancer RNA (eRNA), non-coding natural antisense transcript (ncNAT), and circular RNA (circRNA) and epigenome, a common ground where both protein and RNA species converge to regulate cellular functions. Through several examples, this review provides an overview of the variety of targets, interactors, and mechanisms involved in the RNA-mediated modulation of loci-specific epigenetic states, a fundamental evolutive strategy to orchestrate mammalian gene expression in a timely and reversible manner. We will discuss how RNA-mediated epigenetic regulation impacts development and tissue homeostasis and how its alteration contributes to the onset and progression of many different human diseases, particularly cancer.

Published
2023
Full Text
View/download PDF

24. Ceruloplasmin is a novel adipokine which is overexpressed in adipose tissue of obese subjects and in obesity-associated cancer cells

Author

Arner, E., Forrest, A.R.R., Hideya, K., Michael, R., J Kenneth Baillie, Michiel J, L.D.H., Vanja, H., Timo, L., Ivan, V.K., Marina, L., Masayoshi, I., Robin, A., Christopher, J.M., Terrence, F.M., Sebastian, S., Nicolas, B., Mette, J., Emmanuel, D., Erik, A., Christian, S., Ulf, S., Yulia, A.M., Charles, P., Morana, V., Jessica, S., Colin, A.S., Yuri, I., Margherita, F., Intikhab, A., Davide, A., Gabriel, M.A., John A, C.A., Peter, A., Magda, B., Sarah, B., Piotr, J.B., Anthony, G.B., Swati, P., Judith, A.B., Antje, B., Bodega, B., Alessandro, B., James, B., Frank, B., A Maxwell Burroughs, Andrea, C., Carlo, V.C., Daniel, C., Yun, C., Marco, C., Yari, C., Hans, C.C., Emiliano, D., Carrie, A.D., Michael, D., Alexander, D.D., Taeko, D., Albert S, B.E., Matthias, E., Karl, E., Mitsuhiro, E., Hideki, E., Michela, F., Lynsey, F., Hai, F., Mary, C.F., Geoffrey, J.F., Alexander, V.F., Malcolm, E.F., Martin, C.F., Rie, F., Shiro, F., Cesare, F., Masaaki, F., Jun-ichi, F., Teunis, B.G., Andrew, G., Thomas, G., Daniel, G., Julian, G., Sven, G., Reto, G., Stefano, G., Thomas, J.H., Masahide, H., Mitsuko, H., Matthias, H., Jayson, H., Akira, H., Yuki, H., Takehiro, H., Meenhard, H., Kelly, J.H., Shannan, J.H.S., Oliver, M.H., Ilka, H., Fumi, H., Lukasz, H., Kei, I., Tomokatsu, I., Boris, R.J., Hui, J., Anagha, J., Giuseppe, J., Bogumil, K., Chieko, K., Kaoru, K., Kaiho, A., Kazuhiro, K., Mutsumi, K., Artem, S.K., Takeya, K., Shintaro, K., Sachi, K., Shuji, K., Hiroshi, K., Yuki, I.K., Tsugumi, K., Judith, S.K., Tony, J.K., Juha, K., Levon, M.K., Toshio, K., S Peter Klinken, Alan, J.K., Miki, K., Soichi, K., Naoto, K., Haruhiko, K., Shigeo, K., Sarah, K., Atsutaka, K., Andrew, T.K., Jeroen F, J.L., Weonju, L., Andreas, L., Kang, L., Berit, L., Leonard, L., Alan, M., Ri-ichiroh, M., Jessica, C.M., Benoit, M., Anthony, M., Niklas, M., Alison, M., Yosuke, M., David, A.D.L.M., Hiromasa, M., Mitsuru, M., Kazuyo, M., Efthymios, M., Hozumi, M., Christine, L.M., Mitsuyoshi, M., Sayaka, N., Yutaka, N., Fumio, N., Toshiyuki, N., Yukio, N., Kenichi, N., Erik van Nimwegen, Noriko, N., Hiromi, N., Shohei, N., Tadasuke, N., Soichi, O., Naganari, O., Hiroko, O., Hiroshi, O., Mitsuhiro, O., Mariko, O., Yasushi, O., Valerio, O., Dmitry, A.O., Arnab, P., Robert, P., Margaret, P., Helena, P., Silvano, P., James G, D.P., Owen J, L.R., Jordan, A.R., Mamoon, R., Timothy, R., Patrizia, R., Marco, R., Sugata, R., Morten, B.R., Eri, S., Antti, S., Akiko, S., Shimon, S., Mizuho, S., Hiroki, S., Hironori, S., Suzana, S., Alka, S., Claudio, S., Erik, A.S., Gundula, G.S., Anita, S., Thierry, S., Guojun, S., Hisashi, S., Yishai, S., Jay, W.S., Christophe, S., Daisuke, S., Takaaki, S., Masanori, S., Rolf, K.S., Peter A, C.'.H., Michihira, T., Naoko, T., Jun, T., Hiroshi, T., Hideki, T., Zuotian, T., Mark, T., Hiroo, T., Tetsuro, T., Eivind, V., Marc van de Wetering, Linda, M.V.D.B., Roberto, V., Dipti, V., Ilya, E.V., Wyeth, W.W., Shoko, W., Christine, A.W., Louise, N.W., Ernst, W., Emily, J.W., Yoko, Y., Masayuki, Y., Misako, Y., Yohei, Y., Shigehiro, Y., Suzan, E.Z., Peter, G.Z., Xiaobei, Z., Silvia, Z., Kim, M.S., Harukazu, S., Carsten, O.D., Jun, K., Peter, H., Winston, H., Tom, C.F., Boris, L., Vladimir, B.B., Martin, S.T., Vsevolod, J.M., Albin, S., David, A.H., Piero, C., Yoshihide HayashizakiEhrlund, A., Mejhert, N., Itoh, M., Kawaji, H., Lassmann, T., Laurencikiene, J., Ryden, M., Arner, P., Hubrecht Institute for Developmental Biology and Stem Cell Research, AII - Amsterdam institute for Infection and Immunity, Infectious diseases, and Experimental Immunology

Subjects
Male, Angiogenesis, lcsh:Medicine, Adipose tissue, Gene Expression, Endocrinology, Neoplasms, Molecular Cell Biology, Basic Cancer Research, Databases, Genetic, Medicine and Health Sciences, Adipocytes, lcsh:Science, Multidisciplinary, biology, Cancer Risk Factors, Ceruloplasmin, Genomics, Middle Aged, Genomic Databases, Functional Genomics, Oncology, Adipose Tissue, Female, Transcriptome Analysis, Network Analysis, Research Article, Signal Transduction, Adult, medicine.medical_specialty, Computer and Information Sciences, Adipose tissue macrophages, Adipokine, Settore BIO/11 - Biologia Molecolare, Adipokines, Internal medicine, Cell Line, Tumor, medicine, Genetics, Cancer Genetics, Humans, Obesity, Sweden, business.industry, Gene Expression Profiling, lcsh:R, Cancer, Biology and Life Sciences, Computational Biology, Cell Biology, medicine.disease, Genome Analysis, Signaling Networks, Cell culture, Genetic Loci, Metabolic Disorders, Case-Control Studies, Cancer cell, biology.protein, lcsh:Q, business, Genome Expression Analysis
Abstract

Obesity confers an increased risk of developing specific cancer forms. Although the mechanisms are unclear, increased fat cell secretion of specific proteins (adipokines) may promote/facilitate development of malignant tumors in obesity via cross-talk between adipose tissue(s) and the tissues prone to develop cancer among obese. We searched for novel adipokines that were overexpressed in adipose tissue of obese subjects as well as in tumor cells derived from cancers commonly associated with obesity. For this purpose expression data from human adipose tissue of obese and non-obese as well as from a large panel of human cancer cell lines and corresponding primary cells and tissues were explored. We found expression of ceruloplasmin to be the most enriched in obesity-associated cancer cells. This gene was also significantly up-regulated in adipose tissue of obese subjects. Ceruloplasmin is the body's main copper carrier and is involved in angiogenesis. We demonstrate that ceruloplasmin is a novel adipokine, which is produced and secreted at increased rates in obesity. In the obese state, adipose tissue contributed markedly (up to 22%) to the total circulating protein level. In summary, we have through bioinformatic screening identified ceruloplasmin as a novel adipokine with increased expression in adipose tissue of obese subjects as well as in cells from obesity-associated cancers. Whether there is a causal relationship between adipose overexpression of ceruloplasmin and cancer development in obesity cannot be answered by these cross-sectional comparisons.

Published
2014

25. CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes

Author

Hasegawa, Y., Tang, D., Takahashi, N., Hayashizaki, Y., Forrest, A.R.R., Hideya, K., Michael, R., J Kenneth Baillie, Michiel J, L.D.H., Vanja, H., Timo, L., Ivan, V.K., Marina, L., Masayoshi, I., Robin, A., Christopher, J.M., Terrence, F.M., Sebastian, S., Nicolas, B., Mette, J., Emmanuel, D., Erik, A., Christian, S., Ulf, S., Yulia, A.M., Charles, P., Morana, V., Jessica, S., Colin, A.S., Yuri, I., Robert, S.Y., Margherita, F., Intikhab, A., Davide, A., Gabriel, M.A., Takahiro, A., John A, C.A., Peter, A., Magda, B., Sarah, B., Piotr, J.B., Anthony, G.B., Swati, P., Judith, A.B., Antje, B., Bodega, B., Alessandro, B., James, B., Frank, B., A Maxwell Burroughs, Andrea, C., Carlo, V.C., Daniel, C., Yun, C., Marco, C., Yari, C., Hans, C.C., Emiliano, D., Carrie, A.D., Michael, D., Alexander, D.D., Taeko, D., Finn, D., Albert S, B.E., Matthias, E., Karl, E., Mitsuhiro, E., Hideki, E., Michela, F., Lynsey, F., Hai, F., Mary, C.F., Geoffrey, J.F., Alexander, V.F., Malcolm, E.F., Martin, C.F., Rie, F., Shiro, F., Cesare, F., Masaaki, F., Jun-ichi, F., Teunis, B.G., Andrew, G., Thomas, G., Daniel, G., Julian, G., Sven, G., Reto, G., Stefano, G., Thomas, J.H., Masahide, H., Mitsuko, H., Matthias, H., Jayson, H., Akira, H., Yuki, H., Takehiro, H., Meenhard, H., Kelly, J.H., Shannan, J.H.S., Oliver, M.H., Ilka, H., Fumi, H., Lukasz, H., Kei, I., Tomokatsu, I., Boris, R.J., Hui, J., Anagha, J., Giuseppe, J., Bogumil, K., Chieko, K., Kaoru, K., Kaiho, A., Kazuhiro, K., Mutsumi, K., Artem, S.K., Takeya, K., Shintaro, K., Sachi, K., Shuji, K., Hiroshi, K., Yuki, I.K., Tsugumi, K., Judith, S.K., Tony, J.K., Juha, K., Levon, M.K., Toshio, K., S Peter Klinken, Alan, J.K., Miki, K., Soichi, K., Naoto, K., Haruhiko, K., Shigeo, K., Sarah, K., Atsutaka, K., Andrew, T.K., Jeroen F, J.L., Weonju, L., Andreas, L., Kang, L., Berit, L., Leonard, L., Alan, M., Ri-ichiroh, M., Jessica, C.M., Benoit, M., Anthony, M., Niklas, M., Alison, M., Yosuke, M., David, A.D.L.M., Hiromasa, M., Mitsuru, M., Kazuyo, M., Efthymios, M., Hozumi, M., Christine, L.M., Mitsuyoshi, M., Sayaka, N., Yutaka, N., Fumio, N., Toshiyuki, N., Yukio, N., Kenichi, N., Erik van Nimwegen, Noriko, N., Hiromi, N., Shohei, N., Tadasuke, N., Soichi, O., Naganari, O., Hiroko, O., Hiroshi, O., Mitsuhiro, O., Mariko, O., Yasushi, O., Valerio, O., Dmitry, A.O., Arnab, P., Robert, P., Margaret, P., Helena, P., Silvano, P., James G, D.P., Owen J, L.R., Jordan, A.R., Mamoon, R., Timothy, R., Patrizia, R., Marco, R., Sugata, R., Morten, B.R., Eri, S., Antti, S., Akiko, S., Shimon, S., Mizuho, S., Hiroki, S., Hironori, S., Suzana, S., Alka, S., Claudio, S., Erik, A.S., Gundula, G.S., Anita, S., Thierry, S., Guojun, S., Hisashi, S., Yishai, S., Jay, W.S., Christophe, S., Daisuke, S., Takaaki, S., Masanori, S., Naoko, S., Rolf, K.S., Peter A, C.'.H., Michihira, T., Naoko, T., Jun, T., Hiroshi, T., Hideki, T., Zuotian, T., Mark, T., Hiroo, T., Tetsuro, T., Eivind, V., Marc van de Wetering, Linda, M.V.D.B., Roberto, V., Dipti, V., Ilya, E.V., Wyeth, W.W., Shoko, W., Christine, A.W., Louise, N.W., Ernst, W., Emily, J.W., Yoko, Y., Masayuki, Y., Misako, Y., Yohei, Y., Shigehiro, Y., Suzan, E.Z., Peter, G.Z., Xiaobei, Z., Silvia, Z., Kim, M.S., Harukazu, S., Carsten, O.D., Jun, K., Peter, H., Winston, H., Tom, C.F., Boris, L., Vladimir, B.B., Martin, S.T., Vsevolod, J.M., Albin, S., David, A.H., Piero, C., Yoshihide HayashizakSuzuki, H., Hjelt Institute (-2014), Forensic Medicine, PaleOmics Laboratory, Amsterdam institute for Infection and Immunity, Infectious diseases, Experimental Immunology, and Hubrecht Institute for Developmental Biology and Stem Cell Research

Subjects
Stage-Specific Embryonic Antigens, Chromosomal Proteins, Non-Histone, Cellular differentiation, Basic fibroblast growth factor, Gene Expression, LINES, OXYGEN, chemistry.chemical_compound, 0302 clinical medicine, Piperidines, Phosphorylation, STAT3, Induced pluripotent stem cell, Cells, Cultured, Chemokine CCL2, 0303 health sciences, Multidisciplinary, biology, Reverse Transcriptase Polymerase Chain Reaction, PROLIFERATION, Cell Differentiation, 319 Forensic science and other medical sciences, Nanog Homeobox Protein, Immunohistochemistry, Cell Hypoxia, Cell biology, HUMAN EMBRYONIC STEM, GROUND-STATE, Fibroblast Growth Factor 2, INACTIVATION, Signal transduction, Stem cell, Signal Transduction, Pluripotent Stem Cells, STAT3 Transcription Factor, EXPRESSION, Immunoblotting, Induced Pluripotent Stem Cells, Kruppel-Like Transcription Factors, Settore BIO/11 - Biologia Molecolare, Alkenes, Article, Kruppel-Like Factor 4, 03 medical and health sciences, Humans, Janus Kinases, 030304 developmental biology, Homeodomain Proteins, Proteins, Janus Kinase 1, CAP ANALYSIS, SELF-RENEWAL, chemistry, Epiblast, INDUCIBLE FACTOR-I, Immunology, biology.protein, 030217 neurology & neurosurgery
Abstract

Standard culture of human induced pluripotent stem cells (hiPSCs) requires basic Fibroblast Growth Factor (bFGF) to maintain the pluripotent state, whereas hiPSC more closely resemble epiblast stem cells than true naïve state ES which requires LIF to maintain pluripotency. Here we show that chemokine (C-C motif) ligand 2 (CCL2) enhances the expression of pluripotent marker genes through the phosphorylation of the signal transducer and activator of transcription 3 (STAT3) protein. Moreover, comparison of transcriptomes between hiPSCs cultured with CCL2 versus with bFGF, we found that CCL2 activates hypoxia related genes, suggesting that CCL2 enhanced pluripotency by inducing a hypoxic-like response. Further, we show that hiPSCs cultured with CCL2 can differentiate at a higher efficiency than culturing with just bFGF and we show CCL2 can be used in feeder-free conditions in the absence of LIF. Taken together, our finding indicates the novel functions of CCL2 in enhancing its pluripotency in hiPSCs.

Published
2014
Full Text
View/download PDF

26. I TUMORI IN ITALIA - RAPPORTO 2013: Tumori multipli = ITALIAN CANCER FIGURES - REPORT 2013: Multiple tumours

Author

Adamo, Ms, Alessi, D, Aletta, P, Amodio, R, Andreone, S, Angelin, T, Anghinoni, E, Annulli, Ml, Antonini, S, Artioli, Me, Autelitano, M, Balducci, C, Balottari, P, Baracco, M, Battisti, W, Bella, F, Bellatalla, C, Belluardo, C, Benatti, P, Benedetto, G, Benfatto, L, Bernazza, E, Bianconi, F, Biavati, P, Bidoli, E, Birri, S, Bizzoco, S, Bonelli, L, Bonini, A, Borciani, E, Bovo, E, Bozzani, F, Bozzeda, A, Braghiroli, B, Brucculeri, Ma, Brunori, V, Bucalo, G, Bucchi, L, Bugliarello, E, Bulatko, A, Busco, S, Busso, P, Buzzoni, C, Calabretta, L, Caldarella, A, Candela, G, Canu, L, Cappelletti, M, Caprara, L, Carboni, D, Carletti, N, Caroli, S, Carone, S, Cascio, Ma, Cascone, G, Casella, C, Castaing, M, Cecconami, L, Celesia, Mv, Cena, T, Cercato, Mc, Cesaraccio, R, Chiesa, R, Cirilli, C, Civaschi, A, Cocchioni, Mario, Codazzi, T, Cogno, R, Colamartini, A, Colanino Ziino, A, Cometti, I, Contiero, P, Contrino, Ml, Corbinelli, A, Cordaro, C, Corti, M, Costa, A, Costarelli, D, Cremone, L, Crocetti, E, Curatella, S, Cusimano, R, D'Alò, D, D'Angelo, S, Dal Cappello, T, Dal Cin, A, Dal Maso, L, Dall'Acqua, M, Dalsasso, F, Davini, C, De Dottori, M, De Maria, V, De Santis, E, De Valiere, E, Dei Tos AP, Demurtas, G, Devigli, E, Di Felice, E, di Grazia, L, Di Gregorio, C, Di Prima, A, Distefano, R, Doa, N, Domati, F, Fabiano, S, Facchinelli, G, Falcini, F, Falk, M, Fanetti, Ac, Fattoruso, S, Federico, M, Ferrari, L, Ferretti, S, Fidelbo, M, Filipazzi, L, Fiore, Ar, Fiori, G, Foca, F, Forgiarini, O, Frasca, G, Frassoldi, E, Frizza, J, Fusco, M, Gada, D, Garrone, E, Gasparotti, C, Gatti, L, Gaudiano, C, Gennaro, V, Gentilini, M, Gerevini, C, Ghilardi, S, Ghisleni, S, Giacomin, A, Giavazzi, L, Gilardi, F, Giorgetti, S, Giubelli, C, Giuliani, O, Giurdanella, Mc, Gola, G, Goldoni, Ca, Golizia, Mg, Grandi, L, Greco, A, Guarda, L, Guttadauro, A, Guzzinati, S, Iachetta, F, Iannelli, A, Ieni, A, Intrieri, T, Kaleci, S, La Rosa, F, Lando, C, Lavecchia, Am, Lazzarato, F, Leone, A, Leone, R, Lonati, F, Lottero, B, Lucchi, S, Luminari, S, Macci, L, Macerata, V, Madeddu, A, Maffei, S, Maghini, A, Magnani, C, Magnani, G, Magoni, M, Mameli, G, Mancini, S, Mancuso, P, Mangone, L, Manneschi, G, Mannino, R, Mannino, S, Marani, E, Mariani, F, Martorana, C, Marzola, L, Maspero, S, Maule, M, Mazzei, A, Mazzoleni, G, Mazzucco, G, Melcarne, A, Merletti, F, Michiara, M, Migliari, E, Minerba, S, Minicuzzi, A, Mizzi, M, Monetti, D, Morana, G, Moroni, E, Mosso, Ml, Muni, A, Mura, F, Natali, M, Nemcova, L, Nicita, C, Ocello, C, Paci, E, Pala, F, Palumbo, M, Panico, M, Pannozzo, F, Pascucci, Cristiana, Pastore, G, Patriarca, S, Pedroni, M, Pellegri, C, Perrotta, C, Pesce, P, Petrinelli, Am, Petrucci, C, Pezzarossi, A, Piffer, S, Pintori, N, Pirani, M, Pirino, D, Pironi, V, Ponz de Leon, M, Prandi, R, Prazzoli, R, Preto, L, Puleio, M, Puppo, A, Quaglia, A, Quarta, F, Quattrocchi, M, Raho, Am, Ramazzotti, V, Rashid, I, Ravaioli, A, Ravazzolo, B, Ravegnani, M, Reggiani Bonetti, L, Ribaudo, M, Rinaldi, E, Ricci, P, Rizzello, R, Rollo, Pc, Roncucci, L, Rosano, A, Rossi, F, Rossi, G, Rossi, M, Rossini, S, Rosso, S, Rudisi, G, Ruggeri, Mg, Russo, Ag, Russo, M, Sacchettini, C, Sacco, G, Sacerdote, C, Salvatore, S, Salvi, O, Sampietro, G, Sandrini, M, Santucci, C, Scheibel, M, Schiacchitano, S, Sciacca, S, Sciacchitano, C, Scuderi, T, Sechi, O, Seghini, P, Senatore, G, Serafini, G, Serraino, D, Sgargi, P, Sigona, A, Sini, Gm, Sobrato, I, Soddu, M, Solimene, C, Spano, F, Spata, E, Sperduti, I, Staiti, R, Stocco, C, Stracci, F, Sunseri, R, Sardo, As, Tagliabue, G, Tamburo, L, Tamburrino, S, Tanzarella, M, Terracini, B, Tessandori, R, Tisano, F, Tittarelli, A, Tognazzo, S, Torrisi, A, Traina, A, Trapani, C, Tschugguel, B, Tumino, R, Usala, M, Vacirca, S, Valerio, O, Valla, K, Varvarà, M, Vasquez, E, Vassante, B, Vattiato, R, Vercelli, M, Vercellino, Pc, Vicentini, M, Villa, M, Vitale, F, Vitale, Mf, Vitali, B, Vitarelli, Susanna, Zanchi, A, Zanetti, R, Zani, G, Zanier, L, Zappa, M, Zarcone, M, Zevola, A, Zucchetto, A, and Zucchi, A.

Published
2013

27. Malat-1-PRC2-EZH1 interaction supports adaptive oxidative stress dependent epigenome remodeling in skeletal myotubes

Author

Nadine Hosny El Said, Francesco Della Valle, Peng Liu, Andreu Paytuví-Gallart, Sabir Adroub, Juliette Gimenez, and Valerio Orlando

Subjects
Cytology, QH573-671
Abstract

Abstract PRC2-mediated epigenetic function involves the interaction with long non-coding RNAs (lncRNAs). Although the identity of some of these RNAs has been elucidated in the context of developmental programs, their counterparts in postmitotic adult tissue homeostasis remain uncharacterized. To this aim, we used terminally differentiated postmitotic skeletal muscle cells in which oxidative stress induces the dynamic activation of PRC2-Ezh1 through Embryonic Ectoderm Develpment (EED) shuttling to the nucleus. We identify lncRNA Malat-1 as a necessary partner for PRC2-Ezh1-dependent response to oxidative stress. We show that in this pathway, PRC2-EZH1 dynamic assembly, and in turn stress induced skeletal muscle targeted genes repression, depends specifically on Malat-1. Our study reports about PRC2–RNA interactions in the physiological context of adaptive oxidative stress response and identifies the first lncRNA involved in PRC2-Ezh1 function.

Published
2021
Full Text
View/download PDF

29. Whole cell three phase bioreactors allow for effective production of fatty acid alkyl esters derived from microalgae lipids

Author

Araya, K., Ugarte, A., Azócar, L., Valerio, O., Wick, Lukas, Ciudad, G., Araya, K., Ugarte, A., Azócar, L., Valerio, O., Wick, Lukas, and Ciudad, G.

Abstract

Lipids of the microalgae Botryococcus braunii and Nannochloropsis gaditana were used in a novel whole cell three-phase bioreactor (WCTB) using Rhizopus oryzae as biocatalyst for biodiesel (fatty acid alkyl esters, FAAE) production. The effects varying running conditions (the microalgae lipid, type of acyl acceptor, temperature, nutrient availability, alcohol to lipid ratio, and agar surface area exposed to gas-phase alcohol of varying convective fluxes) on the FAAE yield were studied and optimal conditions for the reactor identified. We found that the complexity of carbon source used (lipids) was related to the efficiency of the process, since it is used for both, growth and FAAE production, hence N. gaditana lipids were a more suitable raw material for FAAE production compared to B. braunii lipids due their higher content of fatty acid. The optimal operational conditions for our work were 20 °C and 8:1 alcohol to lipid volumetric ratio, where the best results was obtained using N. gaditana lipids as raw material and ethanol as acyl acceptor, reaching a FAAE yield of 92%. The results of our work indicate that both mass transfer process and the availability of nutrients are the most important variables in the performance of WCTB, where the hyphae have an important role in mass transfer since they may acts as ‘pipeline’, connecting different phases with the different components of the three phase bioreactor. These results should be considered as key design factors for developing the three phase bioreactor configuration.

Published
2014

30. A Quasi Dynamic Model Applied to a Ramp Load Increase Study

Author

Pedro Paulo Balestrassi, B. Isaias Lima Lopes, Valerio O. Albuquerque, Otavio A. S. Carpinteiro, A. C. Zambroni de Souza, R. Coradi Leme, and Rodnei Dias dos Anjos

Subjects
Engineering, business.industry, Voltage control, Load modeling, Preventive control, Voltage collapse, Term (time), Set (abstract data type), symbols.namesake, Control theory, symbols, Transient (oscillation), business, Newton's method
Abstract

This paper employs a quasi dynamic model for transient and long term analysis. The set of equations considered permits to calculate the solution by using the Newton-Raphson´s method. The simplicity of the model allows one to obtain some pieces of information required to take preventive control actions. A particular case of interest is the ramp load increase. For this sake, a real Brazilian system is employed, so the transient, long term and voltage collapse studies are carried out with all limits considered.

Published
2009
Full Text
View/download PDF

31. Application of Gene Expression Trajectories Initiated from ErbB Receptor Activation Highlights the Dynamics of Divergent Promoter Usage

Author

Alistair R, R.F., Hideya, K., Michael, R., J Kenneth Baillie, Michiel J, L.D.H., Vanja, H., Timo, L., Ivan, V.K., Marina, L., Masayoshi, I., Robin, A., Christopher, J.M., Terrence, F.M., Sebastian, S., Nicolas, B., Mette, J., Emmanuel, D., Erik, A., Christian, S., Ulf, S., Yulia, A.M., Charles, P., Morana, V., Jessica, S., Colin, A.S., Yuri, I., Margherita, F., Intikhab, A., Davide, A., Gabriel, M.A., John A, C.A., Peter, A., Magda, B., Sarah, B., Piotr, J.B., Anthony, G.B., Swati, P., Judith, A.B., Antje, B., Bodega, B., Alessandro, B., James, B., Frank, B., A Maxwell Burroughs, Andrea, C., Carlo, V.C., Daniel, C., Yun, C., Marco, C., Yari, C., Hans, C.C., Emiliano, D., Carrie, A.D., Bart, D., Michael, D., Alexander, D.D., Taeko, D., Finn, D., Albert S, B.E., Matthias, E., Karl, E., Mitsuhiro, E., Hideki, E., Michela, F., Lynsey, F., Hai, F., Mary, C.F., Geoffrey, J.F., Alexander, V.F., Malcolm, E.F., Martin, C.F., Rie, F., Shiro, F., Cesare, F., Masaaki, F., Jun-ichi, F., Teunis, B.G., Andrew, G., Thomas, G., Daniel, G., Julian, G., Sven, G., Reto, G., Stefano, G., Thomas, J.H., Masahide, H., Mitsuko, H., Matthias, H., Jayson, H., Akira, H., Yuki, H., Takehiro, H., Meenhard, H., Kelly, J.H., Shannan, J.H.S., Oliver, M.H., Ilka, H., Fumi, H., Lukasz, H., Kei, I., Tomokatsu, I., Boris, R.J., Hui, J., Anagha, J., Giuseppe, J., Bogumil, K., Chieko, K., Kaoru, K., Kaiho, A., Kazuhiro, K., Mutsumi, K., Artem, S.K., Takeya, K., Shintaro, K., Sachi, K., Shuji, K., Hiroshi, K., Yuki, I.K., Tsugumi, K., Judith, S.K., Tony, J.K., Juha, K., Levon, M.K., Toshio, K., S Peter Klinken, Alan, J.K., Miki, K., Soichi, K., Naoto, K., Haruhiko, K., Shigeo, K., Sarah, K., Atsutaka, K., Andrew, T.K., Jeroen F, J.L., Weonju, L., Andreas, L., Kang, L., Berit, L., Leonard, L., Alan, M., Ri-ichiroh, M., Jessica, C.M., Benoit, M., Anthony, M., Niklas, M., Alison, M., Yosuke, M., David, A.D.L.M., Hiromasa, M., Mitsuru, M., Kazuyo, M., Efthymios, M., Hozumi, M., Christine, L.M., Mitsuyoshi, M., Sayaka, N., Yutaka, N., Fumio, N., Toshiyuki, N., Yukio, N., Kenichi, N., Erik van Nimwegen, Noriko, N., Hiromi, N., Shohei, N., Tadasuke, N., Soichi, O., Naganari, O., Hiroko, O., Hiroshi, O., Mitsuhiro, O., Mariko, O., Yasushi, O., Valerio, O., Dmitry, A.O., Arnab, P., Robert, P., Margaret, P., Helena, P., Silvano, P., James G, D.P., Owen J, L.R., Jordan, A.R., Mamoon, R., Timothy, R., Patrizia, R., Marco, R., Sugata, R., Morten, B.R., Eri, S., Antti, S., Akiko, S., Shimon, S., Mizuho, S., Hiroki, S., Hironori, S., Suzana, S., Alka, S., Claudio, S., Erik, A.S., Gundula, G.S., Anita, S., Thierry, S., Guojun, S., Hisashi, S., Yishai, S., Jay, W.S., Christophe, S., Daisuke, S., Takaaki, S., Masanori, S., Rolf, K.S., Peter A, C.'.H., Michihira, T., Naoko, T., Jun, T., Hiroshi, T., Hideki, T., Zuotian, T., Mark, T., Hiroo, T., Tetsuro, T., Eivind, V., Marc van de Wetering, Linda, M.V.D.B., Roberto, V., Dipti, V., Ilya, E.V., Wyeth, W.W., Shoko, W., Christine, A.W., Louise, N.W., Ernst, W., Emily, J.W., Yoko, Y., Masayuki, Y., Misako, Y., Yohei, Y., Shigehiro, Y., Suzan, E.Z., Peter, G.Z., Xiaobei, Z., Silvia, Z., Kim, M.S., Harukazu, S., Carsten, O.D., Jun, K., Peter, H., Winston, H., Tom, C.F., Boris, L., Vladimir, B.B., Martin, S.T., Vsevolod, J.M., Albin, S., David, A.H., Piero, C., Yoshihide Hayashizaki Carbajo, D., Magi, S., Itoh, M., Kawaji, H., Lassmann, T., Arner, E., Forrest, A.R.R., Carninci, P., Hayashizaki, Y., Daub, C.O., Okada-Hatakeyama, M., Mar, J.C., Hubrecht Institute for Developmental Biology and Stem Cell Research, Amsterdam institute for Infection and Immunity, Infectious diseases, and Experimental Immunology

Subjects
MAP Kinase Signaling System, Neuregulin-1, Cellular differentiation, lcsh:Medicine, Gene Expression, Settore BIO/11 - Biologia Molecolare, Apoptosis, Breast Neoplasms, Cell fate determination, Biology, Cell Line, Promoter Regions, Genetic, Cell Line, Tumor, Humans, Cell Cycle, Cell Differentiation, Cell Proliferation, Enzyme Activation, Epidermal Growth Factor, ErbB Receptors, Extracellular Signal-Regulated MAP Kinases, Female, Focal Adhesions, Gene Expression Profiling, Gene Expression Regulation, MCF-7 Cells, Promoter Regions, Genetic, Tumor Suppressor Protein p53, lcsh:Science, Transcription factor, Genetics, Regulation of gene expression, Tumor, Multidisciplinary, lcsh:R, Promoter, FHL2, Cell biology, Gene expression profiling, lcsh:Q, Signal transduction, Research Article
Abstract

Understanding how cells use complex transcriptional programs to alter their fate in response to specific stimuli is an important question in biology. For the MCF-7 human breast cancer cell line, we applied gene expression trajectory models to identify the genes involved in driving cell fate transitions. We modified trajectory models to account for the scenario where cells were exposed to different stimuli, in this case epidermal growth factor and heregulin, to arrive at different cell fates, i.e. proliferation and differentiation respectively. Using genome-wide CAGE time series data collected from the FANTOM5 consortium, we identified the sets of promoters that were involved in the transition of MCF-7 cells to their specific fates versus those with expression changes that were generic to both stimuli. Of the 1,552 promoters identified, 1,091 had stimulus-specific expression while 461 promoters had generic expression profiles over the time course surveyed. Many of these stimulus-specific promoters mapped to key regulators of the ERK (extracellular signal-regulated kinases) signaling pathway such as FHL2 (four and a half LIM domains 2). We observed that in general, generic promoters peaked in their expression early on in the time course, while stimulus-specific promoters tended to show activation of their expression at a later stage. The genes that mapped to stimulus-specific promoters were enriched for pathways that control focal adhesion, p53 signaling and MAPK signaling while generic promoters were enriched for cell death, transcription and the cell cycle. We identified 162 genes that were controlled by an alternative promoter during the time course where a subset of 37 genes had separate promoters that were classified as stimulus-specific and generic. The results of our study highlighted the degree of complexity involved in regulating a cell fate transition where multiple promoters mapping to the same gene can demonstrate quite divergent expression profiles.

Published
2015
Full Text
View/download PDF

32. New insights in the expression of stromal caveolin 1 in breast cancer spread to axillary lymph nodes

Author

Cristian Scatena, Giovanni Fanelli, Giuseppe Nicolò Fanelli, Michele Menicagli, Paolo Aretini, Valerio Ortenzi, Sara Piera Civitelli, Lorenzo Innocenti, Federica Sotgia, Michael P. Lisanti, and Antonio Giuseppe Naccarato

Subjects
Medicine, Science
Abstract

Abstract Recent evidence suggests that a loss of expression of caveolin in the stromal compartment (sCav-1) of human invasive breast carcinoma (IBC) may be a predictor of disease recurrence, metastasis and poor outcome. At present, there is little knowledge regarding the expression of sCav-1 at the metastatic sites. We therefore studied sCav-1 expression in IBCs and in their axillary lymph nodes to seek a correlation with cancer metastasis. 189 consecutive invasive IBCs (53 with axillary lymph node metastases and 136 without) were studied by immunohistochemistry, using a rabbit polyclonal anti-Cav-1 antibody. In IBCs sCav-1 was evaluated in fibroblasts scattered in the tumor stroma whereas in lymph nodes sCav-1 was assessed in fibroblast-like stromal cells. For the first time, we observed a statistically significant progressive loss of sCav-1 from normal/reactive axillary lymph nodes of tumors limited to the breast to metastatic axillary lymph nodes, through normal/reactive axillary lymph nodes of tumors with axillary metastatic spread. These data indicate that Cav-1 expressed by the stromal compartment of lymph nodes, somehow, may possibly contribute to metastatic spread in IBC.

Published
2021
Full Text
View/download PDF

33. A Biodiversity Boost From the Eurasian Beaver (Castor fiber) in Germany’s Oldest National Park

Author

Valerio Orazi, Jonas Hagge, Martin M. Gossner, Jörg Müller, and Marco Heurich

Subjects
multitaxon biodiversity, ecosystem engineer, habitat restoration, Eurasian beaver (Castor fiber), Bavarian Forest National Park, Evolution, QH359-425, Ecology, QH540-549.5
Abstract

Freshwater ecosystems are among the most threatened ecosystems on the planet. Beavers are important engineers in freshwater ecosystems and reintroduction programs have enabled the recovery of beaver populations in several European countries, but the impact on biodiversity conservation is still unclear. We studied the effects of beavers on the terrestrial biodiversity of eight taxonomic groups by comparing beaver ponds with river and forest habitats in a mountain forest ecosystem in Central Europe. Among the 1,166 collected species, 196 occurred exclusively at beaver ponds, 192 in plots at the river, and 156 in the forest plots. More species of conservation concern were found at the beaver ponds (76) than on the river (67) and forest (63) plots. Abundances of bats and birds were higher at the beaver ponds than at the river or forest sites. The number of bird species at the beaver ponds was higher than at the river. The community composition of birds, beetles, and true bugs differed significantly between the beaver ponds and river plots, and for seven taxonomic groups it differed significantly between the beaver ponds and forest plots. An indicator species analysis revealed eight indicator species for the beaver pond but none for the river and forest plots. Our results demonstrate that beavers, as ecological engineers, increase habitat heterogeneity in mountain forests and thereby promote biodiversity. The expansion of beaver populations into these ecosystems should thus be supported, as it may serve as a biotic restoration tool.

Published
2022
Full Text
View/download PDF

34. Main roads and land cover shaped the genetic structure of a Mediterranean island wild boar population

Author

Roberta Lecis, Olivia Dondina, Valerio Orioli, Daniela Biosa, Antonio Canu, Giulia Fabbri, Laura Iacolina, Antonio Cossu, Luciano Bani, Marco Apollonio, and Massimo Scandura

Subjects
gene flow, landscape genetics, microsatellites, population structure, Sardinia, Sus scrofa meridionalis, Ecology, QH540-549.5
Abstract

Abstract Patterns of genetic differentiation within and among animal populations might vary due to the simple effect of distance or landscape features hindering gene flow. An assessment of how landscape connectivity affects gene flow can help guide management, especially in fragmented landscapes. Our objective was to analyze population genetic structure and landscape genetics of the native wild boar (Sus scrofa meridionalis) population inhabiting the island of Sardinia (Italy), and test for the existence of Isolation‐by‐Distance (IBD), Isolation‐by‐Barrier (IBB), and Isolation‐by‐Resistance (IBR). A total of 393 Sardinian wild boar samples were analyzed using a set of 16 microsatellite loci. Signals of genetic introgression from introduced non‐native wild boars or from domestic pigs were revealed by a Bayesian cluster analysis including 250 reference individuals belonging to European wild populations and domestic breeds. After removal of introgressed individuals, genetic structure in the population was investigated by different statistical approaches, supporting a partition into five discrete subpopulations, corresponding to five geographic areas on the island: north‐west (NW), central west (CW), south‐west (SW), north‐central east (NCE), and south‐east (SE). To test the IBD, IBB, and IBR hypotheses, we optimized resistance surfaces using genetic algorithms and linear mixed‐effects models with a maximum likelihood population effects parameterization. Landscape genetics analyses revealed that genetic discontinuities between subpopulations can be explained by landscape elements, suggesting that main roads, urban settings, and intensively cultivated areas are hampering gene flow (and thus individual movements) within the Sardinian wild boar population. Our results reveal how human‐transformed landscapes can affect genetic connectivity even in a large‐sized and highly mobile mammal such as the wild boar, and provide crucial information to manage the spread of pathogens, including the African Swine Fever virus, endemic in Sardinia.

Published
2022
Full Text
View/download PDF

35. An integrated software development methodology based on hierarchical colored Petri nets

Author

Valerio O. Pinci and Robert M. Shapiro

Subjects
business.industry, Computer science, System requirements specification, computer.file_format, Process architecture, Petri net, Software development process, Software, Systems design, Executable, Software engineering, business, computer, Requirements analysis
Abstract

This paper presents a new integrated software development methodology that uses SADT diagrams, Hierarchical CP-nets, Standard ML, and automatic mechanisms for translating SADT diagrams into Hierarchical CP-nets and for converting Hierarchical CP-nets into Standard ML executable code. A case study describes the development of a software application called the Debt Manager's Assistant, which implements a non-FIFO strategy for electronic funds transfer. This application was built for the Marine Midland Bank of New York and Societe Generale. During application development, the requirements analysis and system specification were done with the aid of the Structured Analysis and Design Technique. The system design and verification used Hierarchical Colored Petri Nets and the implementation was supported with the automatic production of executable Standard Meta Language code.

Published
2005
Full Text
View/download PDF

37. Italian cancer figures, report 2012: Cancer in children and adolescents

Author

Pisani, P, Buzzoni, C, Crocetti, E, Maso, L, Rondelli, R, Alessi, D, Aricò, M, Bidoli, E, Ferrari, A, Fusco, M, Gatta, G, Guzzinati, S, Jankovic, M, Locatelli, F, Maule, M, Mosso, M, Pastore, G, Pession, A, Rashid, I, Sacerdote, C, Terracini, B, Tognazzo, S, Trama, A, Ferretti, S, Porta, F, Mazzoleni, G, Tschugguel, B, De Valiere, E, Facchinelli, G, Falk, M, Cappello, T, Giacomin, A, Vercellino, P, Andreone, S, Marzola, L, Migliari, E, Carletti, N, Nenci, I, Caldarella, A, Corbinelli, A, Intrieri, T, Manneschi, G, Nemcova, L, Ocello, C, Sacchettini, C, Zappa, M, Paci, E, Serraino, D, Angelin, T, Birri, S, De Dottori, M, De Santis, E, Forgiarini, O, Zucchetto, A, Zanier, L, Vercelli, M, Orengo, M, Casella, C, Marani, E, Puppo, A, Celesia, M, Cogno, R, Levrieri, I, Manenti, S, Garrone, E, Quaglia, A, Pannozzo, F, Busco, S, Ramazzotti, V, Cercato, M, Battisti, W, Sperduti, I, Macci, L, Bugliarello, E, Valerio, O, Bernazza, E, Tamburo, L, Rossi, M, Curatella, S, Tamburrino, S, Fattoruso, S, Bisanti, L, Autelitano, M, Cuccaro, F, Randi, G, Ghilardi, S, Leone, R, Filipazzi, L, Bonini, A, Giubelli, C, Federico, M, Artioli, M, Valla, K, Braghiroli, B, Cirilli, C, Luminari, S, Pirani, M, Ferrari, L, Bellatalla, C, Panico, M, Perrotta, C, Vassante, B, Vitale, M, Michiara, M, Bozzani, F, Sgargi, P, Tumino, R, Rosa, M, Cascone, G, Frasca, G, Giurdanella, M, Martorana, C, Morana, G, Nicita, C, Rollo, P, Ruggeri, M, Sigona, A, Spata, E, Vacirca, S, Mangone, L, Di Felice, E, Pezzarossi, A, Caroli, S, Pellegri, C, Vicentini, M, D'Angelo, S, Balottari, P, Romagna, R, Falcini, F, Colamartini, A, Bucchi, L, Balducci, C, Ravegnani, M, Vitali, B, Cordaro, C, Caprara, L, Giuliani, O, Giorgetti, S, Palumbo, M, Vattiato, R, Ravaioli, A, Mancini, S, Tonelli, C, Amadori, M, Cremone, L, Iannelli, A, Senatore, G, Zevola, A, Sechi, O, Budroni, M, Cesaraccio, R, Pirino, D, Carboni, D, Fiori, G, Soddu, M, Mameli, G, Mura, F, Contrino, M, Madeddu, A, Tisano, F, Sciacca, S, Muni, A, Mizzi, M, Russo, M, Sacco, G, Aletta, P, Ziino, A, Tessandori, R, Fanetti, A, Maspero, S, Cecconami, L, Annulli, M, Moroni, E, Gonzalez, M, Zanetti, R, Rosso, S, Patriarca, S, Prandi, R, Sobrato, I, Gilardi, F, Busso, P, Piffer, S, Rizzello, R, Gentilini, M, Cappelletti, M, Rosa, F, Stracci, F, D'Alò, D, Scheibel, M, Costarelli, D, Spano, F, Rossini, S, Santucci, C, Petrinelli, A, Solimene, C, Bianconi, F, Brunori, V, Crosignani, P, Tagliabue, G, Contiero, P, Preto, L, Tittarelli, A, Maghini, A, Codazzi, T, Frassoldi, E, Gada, D, Costa, E, Grazia, L, Zambon, P, Baracco, M, Bovo, E, Cin, A, Fiore, A, Greco, A, Monetti, D, Rosano, A, Stocco, C, Donato, F, Limina, R, Adorni, A, Andreis, P, Zani, G, Piovani, F, Salvi, O, Puleio, M, Vitarelli, S, Candela, G, Pappalardo, G, Scuderi, T, Lottero, B, Ribaudo, M, Ricci, P, Guarda, L, Gatti, L, Bozzeda, A, Dall'Acqua, M, Pironi, V, Sardo, A, Mazzei, A, Sirianni, N, Lavecchia, A, Mancuso, P, Vitale, F, Cusimano, R, Amodio, R, Guttadauro, A, Ravazzolo, B, Bucalo, G, Trapani, C, Sunseri, R, Cascio, M, Brucculeri, M, Adamo, M, Mannino, R, Mistretta, A, Costa, A, Rudisi, G, Gola, G, Corti, M, Prosperini, E, Grandi, L, Sciacchitano, S, Fidelbo, M, Benedetto, G, Vasquez, E, Torrisi, A, Pesce, P, Calabretta, L, Bella, F, Leone, A, Ieni, A, Varvarà, M, Castaing, M, Di Prima, A, Benedetto, A, Paderni, F, Usala, M, Pala, F, Sini, G, Pintori, N, Canu, L, Demurtas, G, Doa, N, Lazzarato, F, Magnani, C, Merletti, F, Sarcerdote, C, Macerata, V, de Leon, M, Domati, F, Rossi, G, Goldoni, C, Rossi, F, De Gaetani, C, Benatti, P, Roncucci, L, Di Gregorio, C, Pedroni, M, Pezzi, A, Maffei, S, Mariani, F, Borsi, E, Cocchioni, M, Pascucci, C, Gennaro, V, Lazzarotto, A, Benfatto, L, Mazzucco, G, Montanaro, F, Traina, A, Carruba, G, Dolcemascolo, C, Staiti, R, Zarcone, M, Bisogno, G, Cuttini, M, De Rosa, M, Haupt, R, Soresina, A, Valsecchi, M, Fagioli, F, Bona, G, Dini, G, Conter, V, Biondi, A, Zecca, M, Fedeli, F, Massimino, M, Nespoli, L, Roncarolo, M, Basso, G, Cesaro, S, Memo, L, Bellettato, M, Tamaro, P, Mascarin, M, Rosolen, A, Izzi, G, Paolucci, P, Borgna Pignatti, C, Vecchi, V, Abate, M, D'Ambrosio, A, Favre, C, Caniglia, M, Pierani, P, Felici, L, Visani, G, Fioritoni, G, Foà, R, Riccardi, R, Frega, G, Clerico, A, Casale, F, Poggi, V, Amendola, G, Filosa, A, Ladogana, S, Presta, G, Tornesello, A, De Mattia, D, Consarino, C, Nobile, F, Sperlì, D, D'Angelo, P, Marino, S, Cosmi, C, Mura, R, Battisti, L, Maso, LD, Mosso, ML, Cappello, TD, Vercellino, PC, Orengo, MA, Celesia, MV, Cercato, MC, Artioli, ME, Vitale, MF, Rosa, MG, Giurdanella, MC, Rollo, PC, Ruggeri, MG, Romagna, Rt, Contrino, ML, Ziino, AC, Fanetti, AC, Annulli, ML, Gonzalez, ME, Gentilini, MA, Rosa, FL, Petrinelli, AM, Grazia, Ld, Cin, AD, Fiore, AR, Limina, RM, Sardo, AS, Lavecchia, AM, Cascio, MA, Brucculeri, MA, Adamo, MS, Sini, GM, de Leon, MP, Goldoni, CA, VALSECCHI, MARIA GRAZIA, BIONDI, ANDREA, Roncarolo, MG, Abate, ME, Mura, RM, Battisti, L., Pisani, P, Buzzoni, C, Crocetti, E, Maso, L, Rondelli, R, Alessi, D, Aricò, M, Bidoli, E, Ferrari, A, Fusco, M, Gatta, G, Guzzinati, S, Jankovic, M, Locatelli, F, Maule, M, Mosso, M, Pastore, G, Pession, A, Rashid, I, Sacerdote, C, Terracini, B, Tognazzo, S, Trama, A, Ferretti, S, Porta, F, Mazzoleni, G, Tschugguel, B, De Valiere, E, Facchinelli, G, Falk, M, Cappello, T, Giacomin, A, Vercellino, P, Andreone, S, Marzola, L, Migliari, E, Carletti, N, Nenci, I, Caldarella, A, Corbinelli, A, Intrieri, T, Manneschi, G, Nemcova, L, Ocello, C, Sacchettini, C, Zappa, M, Paci, E, Serraino, D, Angelin, T, Birri, S, De Dottori, M, De Santis, E, Forgiarini, O, Zucchetto, A, Zanier, L, Vercelli, M, Orengo, M, Casella, C, Marani, E, Puppo, A, Celesia, M, Cogno, R, Levrieri, I, Manenti, S, Garrone, E, Quaglia, A, Pannozzo, F, Busco, S, Ramazzotti, V, Cercato, M, Battisti, W, Sperduti, I, Macci, L, Bugliarello, E, Valerio, O, Bernazza, E, Tamburo, L, Rossi, M, Curatella, S, Tamburrino, S, Fattoruso, S, Bisanti, L, Autelitano, M, Cuccaro, F, Randi, G, Ghilardi, S, Leone, R, Filipazzi, L, Bonini, A, Giubelli, C, Federico, M, Artioli, M, Valla, K, Braghiroli, B, Cirilli, C, Luminari, S, Pirani, M, Ferrari, L, Bellatalla, C, Panico, M, Perrotta, C, Vassante, B, Vitale, M, Michiara, M, Bozzani, F, Sgargi, P, Tumino, R, Rosa, M, Cascone, G, Frasca, G, Giurdanella, M, Martorana, C, Morana, G, Nicita, C, Rollo, P, Ruggeri, M, Sigona, A, Spata, E, Vacirca, S, Mangone, L, Di Felice, E, Pezzarossi, A, Caroli, S, Pellegri, C, Vicentini, M, D'Angelo, S, Balottari, P, Romagna, R, Falcini, F, Colamartini, A, Bucchi, L, Balducci, C, Ravegnani, M, Vitali, B, Cordaro, C, Caprara, L, Giuliani, O, Giorgetti, S, Palumbo, M, Vattiato, R, Ravaioli, A, Mancini, S, Tonelli, C, Amadori, M, Cremone, L, Iannelli, A, Senatore, G, Zevola, A, Sechi, O, Budroni, M, Cesaraccio, R, Pirino, D, Carboni, D, Fiori, G, Soddu, M, Mameli, G, Mura, F, Contrino, M, Madeddu, A, Tisano, F, Sciacca, S, Muni, A, Mizzi, M, Russo, M, Sacco, G, Aletta, P, Ziino, A, Tessandori, R, Fanetti, A, Maspero, S, Cecconami, L, Annulli, M, Moroni, E, Gonzalez, M, Zanetti, R, Rosso, S, Patriarca, S, Prandi, R, Sobrato, I, Gilardi, F, Busso, P, Piffer, S, Rizzello, R, Gentilini, M, Cappelletti, M, Rosa, F, Stracci, F, D'Alò, D, Scheibel, M, Costarelli, D, Spano, F, Rossini, S, Santucci, C, Petrinelli, A, Solimene, C, Bianconi, F, Brunori, V, Crosignani, P, Tagliabue, G, Contiero, P, Preto, L, Tittarelli, A, Maghini, A, Codazzi, T, Frassoldi, E, Gada, D, Costa, E, Grazia, L, Zambon, P, Baracco, M, Bovo, E, Cin, A, Fiore, A, Greco, A, Monetti, D, Rosano, A, Stocco, C, Donato, F, Limina, R, Adorni, A, Andreis, P, Zani, G, Piovani, F, Salvi, O, Puleio, M, Vitarelli, S, Candela, G, Pappalardo, G, Scuderi, T, Lottero, B, Ribaudo, M, Ricci, P, Guarda, L, Gatti, L, Bozzeda, A, Dall'Acqua, M, Pironi, V, Sardo, A, Mazzei, A, Sirianni, N, Lavecchia, A, Mancuso, P, Vitale, F, Cusimano, R, Amodio, R, Guttadauro, A, Ravazzolo, B, Bucalo, G, Trapani, C, Sunseri, R, Cascio, M, Brucculeri, M, Adamo, M, Mannino, R, Mistretta, A, Costa, A, Rudisi, G, Gola, G, Corti, M, Prosperini, E, Grandi, L, Sciacchitano, S, Fidelbo, M, Benedetto, G, Vasquez, E, Torrisi, A, Pesce, P, Calabretta, L, Bella, F, Leone, A, Ieni, A, Varvarà, M, Castaing, M, Di Prima, A, Benedetto, A, Paderni, F, Usala, M, Pala, F, Sini, G, Pintori, N, Canu, L, Demurtas, G, Doa, N, Lazzarato, F, Magnani, C, Merletti, F, Sarcerdote, C, Macerata, V, de Leon, M, Domati, F, Rossi, G, Goldoni, C, Rossi, F, De Gaetani, C, Benatti, P, Roncucci, L, Di Gregorio, C, Pedroni, M, Pezzi, A, Maffei, S, Mariani, F, Borsi, E, Cocchioni, M, Pascucci, C, Gennaro, V, Lazzarotto, A, Benfatto, L, Mazzucco, G, Montanaro, F, Traina, A, Carruba, G, Dolcemascolo, C, Staiti, R, Zarcone, M, Bisogno, G, Cuttini, M, De Rosa, M, Haupt, R, Soresina, A, Valsecchi, M, Fagioli, F, Bona, G, Dini, G, Conter, V, Biondi, A, Zecca, M, Fedeli, F, Massimino, M, Nespoli, L, Roncarolo, M, Basso, G, Cesaro, S, Memo, L, Bellettato, M, Tamaro, P, Mascarin, M, Rosolen, A, Izzi, G, Paolucci, P, Borgna Pignatti, C, Vecchi, V, Abate, M, D'Ambrosio, A, Favre, C, Caniglia, M, Pierani, P, Felici, L, Visani, G, Fioritoni, G, Foà, R, Riccardi, R, Frega, G, Clerico, A, Casale, F, Poggi, V, Amendola, G, Filosa, A, Ladogana, S, Presta, G, Tornesello, A, De Mattia, D, Consarino, C, Nobile, F, Sperlì, D, D'Angelo, P, Marino, S, Cosmi, C, Mura, R, Battisti, L, Maso, LD, Mosso, ML, Cappello, TD, Vercellino, PC, Orengo, MA, Celesia, MV, Cercato, MC, Artioli, ME, Vitale, MF, Rosa, MG, Giurdanella, MC, Rollo, PC, Ruggeri, MG, Romagna, Rt, Contrino, ML, Ziino, AC, Fanetti, AC, Annulli, ML, Gonzalez, ME, Gentilini, MA, Rosa, FL, Petrinelli, AM, Grazia, Ld, Cin, AD, Fiore, AR, Limina, RM, Sardo, AS, Lavecchia, AM, Cascio, MA, Brucculeri, MA, Adamo, MS, Sini, GM, de Leon, MP, Goldoni, CA, VALSECCHI, MARIA GRAZIA, BIONDI, ANDREA, Roncarolo, MG, Abate, ME, Mura, RM, and Battisti, L.

Abstract

This study describes up-to-date cancer incidence and survival in Italian paediatric and adolescent patients, based on data collected by the network of Italian cancer registries (AIRTUM). It updates the monograph published on the same topic in 2008. The main objective of this monograph is to present the statistics according to standard rigorous epidemiological methods and disseminate them to a wide range of readers, including the lay public. Given the deep impact of the 2008 monograph on the general public, in this update we complement descriptive statistics with additional data and commentaries on issues of importance for public health, in order to provide unambiguous criteria on how to interpret the statistics. The study is the result of the collaboration between AIRTUM and AIEOP (Italian Association of Paediatric Haematology and Oncology) with contributions from interested parties, including representatives of parent associations. The monograph is divided into three parts. The first part presents incidence rates, survival probabilities, and time trends, by sex, age, geographical area, and cancer site or type, by means of tables and graphs as in the previous monograph, to facilitate direct comparisons. Four articles summarize and comment the results. The second part uses data from AIRTUM and AIEOP to outline patient management and health care issues; it includes estimates of the number of new cases in the next decade and of young adults living after a paediatric cancer diagnosis. Health organizational aspects of treatment services for paediatric patients, based on the AIEOP database, are also discussed, along with long-term complications in cured patients. The third section describes the changes in mortality trends due to improving therapies and healthcare services, and discusses risk factors and prevention of childhood cancer, late adverse events in cured patients, and other related issues.

Published
2013

38. RADICL-seq identifies general and cell type–specific principles of genome-wide RNA-chromatin interactions

Author

Alessandro Bonetti, Federico Agostini, Ana Maria Suzuki, Kosuke Hashimoto, Giovanni Pascarella, Juliette Gimenez, Leonie Roos, Alex J. Nash, Marco Ghilotti, Christopher J. F. Cameron, Matthew Valentine, Yulia A. Medvedeva, Shuhei Noguchi, Eneritz Agirre, Kaori Kashi, Samudyata, Joachim Luginbühl, Riccardo Cazzoli, Saumya Agrawal, Nicholas M. Luscombe, Mathieu Blanchette, Takeya Kasukawa, Michiel de Hoon, Erik Arner, Boris Lenhard, Charles Plessy, Gonçalo Castelo-Branco, Valerio Orlando, and Piero Carninci

Subjects
Science
Abstract

Mammalian genomes encode tens of thousands of ncRNAs that have important roles in regulation of gene expression and chromatin organization. Here, the authors present RADICLseq to map RNA-chromatin interactions in intact nuclei to shed light on these fine-tuned processes.

Published
2020
Full Text
View/download PDF

39. Transdifferentiation of Mouse Embryonic Fibroblasts into Dopaminergic Neurons Reactivates LINE-1 Repetitive Elements

Author

Francesco Della Valle, Manjula P. Thimma, Massimiliano Caiazzo, Salvatore Pulcrano, Mirko Celii, Sabir A. Adroub, Peng Liu, Gregorio Alanis-Lobato, Vania Broccoli, and Valerio Orlando

Subjects
Medicine (General), R5-920, Biology (General), QH301-705.5
Abstract

Summary: In mammals, LINE-1 (L1) retrotransposons constitute between 15% and 20% of the genome. Although only a few copies have retained the ability to retrotranspose, evidence in brain and differentiating pluripotent cells indicates that L1 retrotransposition occurs and creates mosaics in normal somatic tissues. The function of de novo insertions remains to be understood. The transdifferentiation of mouse embryonic fibroblasts to dopaminergic neuronal fate provides a suitable model for studying L1 dynamics in a defined genomic and unaltered epigenomic background. We found that L1 elements are specifically re-expressed and mobilized during the initial stages of reprogramming and that their insertions into specific acceptor loci coincides with higher chromatin accessibility and creation of new transcribed units. Those events accompany the maturation of neuronal committed cells. We conclude that L1 retrotransposition is a non-random process correlating with chromatin opening and lncRNA production that accompanies direct somatic cell reprogramming. : In this article, Orlando and colleagues show that LINE-1 elements are reactivated and retrotransposed upon transdifferentiation of MEF cells into induced dopaminergic neurons (iDA). The inhibition of LINE-1 elements impairs the transdifferentiation potential of MEF cells. LINE-1 retrotransposition in post-mitotic iDA cells is lineage specific and correlates with chromatin opening and lncRNA production in intergenic genomic regions. Keywords: LINE-1 elements, cell transdifferentiation, cell plasticity

Published
2020
Full Text
View/download PDF

41. Somatochlora arctica (Odonata: Corduliidae) oviposing at a 'lower than usual' altitude for Italy and the Mediterranean Region and first observation for the Varese Province (Northern Italy)

Author

Stefano Aguzzi and Valerio Orioli

Subjects
Altitudinal record, Anisoptera, endangered species, Mediterranean Region, Botany, QK1-989, Geology, QE1-996.5
Abstract

Somatochlora arctica is an endangered dragonfly with populations characterized by low density and scattered distribution. The presence of the species in the Varese Province, recorded during the specific monitoring operations for Nehalennia speciosa, is reported for the first time. The observation of an oviposing female was carried out in a peat bog placed at 550 m a.s.l., the lowest altitude for the species in Italy and one of the most unusual for the Mediterranean Region. The peculiarities of this record are shown.

Published
2021
Full Text
View/download PDF

42. Modelling the Work Flow of a Nuclear Waste Management Program

Author

Valerio O. Pinci and Kjeld Høyer Mortensen

Subjects
Engineering, business.industry, Event (computing), Systems engineering, Work flow, Radioactive waste, Structured Analysis and Design Technique, Executable, computer.file_format, Petri net, business, computer
Abstract

In this paper we describe a modelling project to improve a nuclear waste management program in charge of the creation of a new system for the permanent disposal of nuclear waste.SADT (Structured Analysis and Design Technique) is used in order to provide a work-flow description of the functions to be performed by the waste management program. This description is then translated into a number of Coloured Petri Nets (CPN or CP-nets) corresponding to different program functions where additional behavioural inscriptions provide basis for simulation. Each of these CP-nets is simulated to produce timed event charts that are useful for understanding the behaviour of the program functions under different scenarios. Then all the CPN models are linked together to form a single stand-alone application that is useful for validating the interaction and cooperation between the different program functions.A technique for linking executable CPN models is developed for supporting large modelling projects and parallel development of independent CPN models.

Published
1997
Full Text
View/download PDF

43. Modelling the work flow of a nuclear waste management program

Author

Valerio O. Pinci and Kjeld Høyer Mortensen

Subjects
Waste management, Hardware_GENERAL, Code segment, Radioactive waste, Environmental science, Work flow, ComputerApplications_COMPUTERSINOTHERSYSTEMS, Petri net, Decision table, Waste management system
Abstract

In this paper we describe a modelling project to improve a nuclear waste management program in charge of the creation of a new system for the permanent disposal of nuclear waste.

Published
1994
Full Text
View/download PDF

44. Modeling a NORAD Command Post using SADT and Colored Petri Nets

Author

Valerio O. Pinci, Roberto Mameli, and Robert M. Shapiro

Subjects
Focus (computing), Computer science, Programming language, Code segment, Colored petri, Automatic translation, Computer support, Structured Analysis and Design Technique, IDEF, computer.software_genre, computer, Task (project management)
Abstract

In this paper we focus on the task of modeling a NORAD Command Post, using SADT (Structured Analysis and Design Technique) and hierarchical Colored Petri Nets (CPN or CP-nets). The paper contains: a description of IDEF/CPN — an extension of SADT that incorporates behavioral information in SADT models. a discussion of the computer support for creating and editing IDEF/CPN models, their automatic translation into CPN models and their subsequent execution and analysis a detailed IDEF/CPN model of a NORAD command post.

Published
1993
Full Text
View/download PDF

45. Ubiquitin ligases HUWE1 and NEDD4 cooperatively control signal-dependent PRC2-Ezh1α/β-mediated adaptive stress response pathway in skeletal muscle cells

Author

Peng Liu, Muhammad Shuaib, Huoming Zhang, Seba Nadeef, and Valerio Orlando

Subjects
HUWE1, NEDD4, Polycomb, Ubiquitination, Genetics, QH426-470
Abstract

Abstract Background While the role of Polycomb group protein-mediated “cell memory” is well established in developmental contexts, little is known about their role in adult tissues and in particular in post-mitotic cells. Emerging evidence assigns a pivotal role in cell plasticity and adaptation. PRC2-Ezh1α/β signaling pathway from cytoplasm to chromatin protects skeletal muscle cells from oxidative stress. However, detailed mechanisms controlling degradation of cytoplasmic Ezh1β and assembly of canonical PRC2-Ezh1α repressive complex remain to be clarified. Results Here, we report NEDD4 ubiquitin E3 ligase, as key regulator of Ezh1β. In addition, we report that ubiquitination and degradation of Ezh1β is controlled by another layer of regulation, that is, one specific phosphorylation of serine 560 located at Ezh1β-specific C terminal. Finally, we demonstrate that also Ezh1α needs to be stabilized under stress condition and this stabilization process requires decreased association pattern between another E3 ubiquitin ligase HUWE1. Conclusions Together, these results shed light on key components that regulate PRC2-Ezh1α/β pathway to direct modulation of epigenome plasticity and transcriptional output in skeletal muscle cells.

Published
2019
Full Text
View/download PDF

46. Winners and losers: How the elevational range of breeding birds on Alps has varied over the past four decades due to climate and habitat changes

Author

Luciano Bani, Massimiliano Luppi, Emanuel Rocchia, Olivia Dondina, and Valerio Orioli

Subjects
climate change, elevational distribution, habitat transformation, hierarchical partitioning, land abandonment, response curve, Ecology, QH540-549.5
Abstract

Abstract Climate warming and habitat transformation are widely recognized as worrying threatening factors. Understanding the individual contribution of these two factors to the change of species distribution could be very important in order to effectively counteract the species range contraction, especially in mountains, where alpine species are strongly limited in finding new areas to be colonized at higher elevations. We proposed a method to disentangle the effects of the two drivers of range change for breeding birds in Italian Alps, in the case of co‐occurring climate warming and shrub and forest encroachment. For each species, from 1982 to 2017, we related the estimated yearly elevational distribution of birds to the correspondent overall average of the daily minimum temperatures during the breeding season and the estimated amount of shrubs and forest cover. Using a hierarchical partitioning approach, we assessed the net contribution (i.e., without the shared effect) of each driver. Both temperature and shrub and forest cover showed a positive trend along the time series and resulted the most likely causes of the significant elevational displacement for 21 of the 29 investigated birds. While shrub and forest cover was found to be an important driver of the expansion of forest bird range toward higher elevations, the effect of temperature on favouring the colonization of previously climatically unsuitable forests at higher elevations was not negligible. Shrub and forest expansion resulted the main driver of the range contraction for edge and open habitat species, which suffered a distribution shrinkage at their lower elevational boundary. In light of climate warming, these results highlighted how the net range loss for edge and open habitat species, caused by shrub and forest encroachment consequent to land abandonment, should be counteracted by implementing proper conservation management strategies and promoting sustainable economic activities in rangeland areas.

Published
2019
Full Text
View/download PDF

47. Estudio sobre Actitudes y Compartimentos que influyen en la Masculinidad desde la Percepción de Estudiantes de la Universidad Nacional de Asunción (Paraguay)

Author

Valerio Olguín Carvallo

Subjects
Roles de género, masculinidad hegemónica, masculinidades, paternidad, sexualidad y salud reproductiva, Sociology (General), HM401-1281, Anthropology, GN1-890
Abstract

Este artículo expone los resultados de una investigación realizada en el año 2016, esta buscó caracterizar la masculinidad tradicional a partir de la percepción de estudiantes de la Universidad Nacional de Asunción en ese mismo año. Entre sus objetivos el estudio también buscó identificar las influencias del sistema patriarcal en estudiantes universitarios a partir de actitudes y comportamientos de varones y mujeres; para lo cual se analizaron dimensiones relacionadas a: los cambios en las nuevas generaciones; las responsabilidades que asumen varones y mujeres en la sexualidad y la salud reproductiva; la paternidad y la violencia física. El diseño metodológico, es de tipo exploratorio descriptivo con un enfoque cualitativo en vista a que para la recolección de datos se utilizó como instrumento la entrevista semiestructurada abierta y cerrada con un cuestionario guía compuesto de 30 preguntas. La muestra estuvo compuesta de 24 entrevistas a estudiantes (12 varones y 12 mujeres) de los últimos años de las carreras de edades entre 23 a 45 años residentes en la ciudad de Asunción y el departamento central, las mismas corresponden a estudiantes de las carreras de Psicología, Trabajo Social, Derecho y Medicina.

Published
2021
Full Text
View/download PDF

48. Modal Identification of Structures with Interacting Diaphragms

Author

Rosario Ceravolo, Erica Lenticchia, Gaetano Miraglia, Valerio Oliva, and Linda Scussolini

Subjects
system identification, simplified analytical models, interacting diaphragms, expansion joints, structural health monitoring, operational modal analysis, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
Abstract

System identification proves in general to be very efficient in the extraction of modal parameters of a structure under ambient vibrations. However, great difficulties can arise in the case of structures composed of many connected bodies, whose mutual interaction may lead to a multitude of coupled modes. In the present work, a methodology to approach the identification of interconnected diaphragmatic structures, exploiting a simplified analytical model, is proposed. Specifically, a parametric analysis has been carried out on a numerical basis on the simplified model, i.e., a multiple spring–mass model. The results were then exploited to aid the identification of a significant case study, represented by the Pavilion V, designed by Riccardo Morandi as a hypogeum hall of the Turin Exhibition Center. The structure is indeed composed of three blocks separated by expansion joints, whose characteristics are unknown. As the main result, light was shed on the contribution of the stiffness of the joints to the global dynamic behavior of structures composed of interacting diaphragms, and, in particular, on the effectiveness of the joints of Pavilion V.

Published
2022
Full Text
View/download PDF

49. Angioleiomyoma of the knee: An uncommon cause of leg pain. A systematic review of the literature

Author

Nicola Montemurro, Valerio Ortenzi, Giuseppe Antonio Naccarato, and Paolo Perrini

Subjects
Angioleiomyoma, Cruralgia, Benign tumor, Surgery, RD1-811, Neurology. Diseases of the nervous system, RC346-429
Abstract

Objective: Angioleiomyoma is a rare benign painful soft tissue tumor, whose knee location is rare. Due its rarity, and not characteristic aspect on MRI the preoperative diagnosis is difficult. Methods: We performed a systematic review of the literature, including a case of venous type angioleiomyoma that we have recently managed. Results: A total of 24 published papers with 30 cases (including our illustrative case) were identified and included in our review. The mean patient age was 42.3 years (range18-63). The average size of the lesion was 17.8 mm. The presenting symptom was leg pain in 90% of cases. On magnetic resonance imaging (MRI), the lesion appeared isointense in T1 in 80% of cases and hyperintense on T2 in 90% of cases. Avid homogeneous enhancement after gadolinium administration was detected in 94% of cases. All patients underwent surgery and total resection was achieved in 100% of cases. No recurrence was observed after a mean follow-up of 19.5 months. Conclusion: Angioleiomyoma occurs rarely in the knee and generally is associated with localized or radiating pain. The preoperative diagnosis is difficult also after completion of MRI study and requires high index of suspicion. Angioleiomyoma widens the spectrum of soft tissue lesions of the extremities and should be included in the differential diagnosis of lesions in this area.

Published
2020
Full Text
View/download PDF

50. The Grasp Strategy of a Robot Passer Influences Performance and Quality of the Robot-Human Object Handover

Author

Valerio Ortenzi, Francesca Cini, Tommaso Pardi, Naresh Marturi, Rustam Stolkin, Peter Corke, and Marco Controzzi

Subjects
human-robot interaction (HRI), human-robot collaboration (HRC), seamless interaction, task-oriented grasping, object handover, Mechanical engineering and machinery, TJ1-1570, Electronic computers. Computer science, QA75.5-76.95
Abstract

Task-aware robotic grasping is critical if robots are to successfully cooperate with humans. The choice of a grasp is multi-faceted; however, the task to perform primes this choice in terms of hand shaping and placement on the object. This grasping strategy is particularly important for a robot companion, as it can potentially hinder the success of the collaboration with humans. In this work, we investigate how different grasping strategies of a robot passer influence the performance and the perceptions of the interaction of a human receiver. Our findings suggest that a grasping strategy that accounts for the subsequent task of the receiver improves substantially the performance of the human receiver in executing the subsequent task. The time to complete the task is reduced by eliminating the need of a post-handover re-adjustment of the object. Furthermore, the human perceptions of the interaction improve when a task-oriented grasping strategy is adopted. The influence of the robotic grasp strategy increases as the constraints induced by the object's affordances become more restrictive. The results of this work can benefit the wider robotics community, with application ranging from industrial to household human-robot interaction for cooperative and collaborative object manipulation.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results