1. Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique.
- Author
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Barione DF, Lizarte FS, Novais PC, de Carvalho CA, Valeri FC, Peria FM, de Oliveira HF, Zanette DL, Silva WA Jr, Cologna AJ, Reis RB, Tucci S Jr, Martins AC, Tirapelli DP, and Tirapelli LF
- Subjects
- Apoptotic Protease-Activating Factor 1 genetics, Apoptotic Protease-Activating Factor 1 metabolism, Caspase 9 genetics, Caspase 9 metabolism, Cytochromes c genetics, Cytochromes c metabolism, Gene Expression Profiling, Humans, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, Real-Time Polymerase Chain Reaction, Apoptosis genetics, Gene Expression Regulation, Neoplastic, Signal Transduction, Urinary Bladder Neoplasms genetics, Urinary Bladder Neoplasms metabolism
- Abstract
We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P < 0.01, P < 0.05, and P < 0.01), low expression of caspase-9 in all groups (P < 0.05), and an increase in cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P < 0.05). The results proved that there is low evidence of apoptotic activity by the intrinsic pathway, demonstrated by the low expression of caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment.
- Published
- 2013
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