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Objective: To evaluate and determine the anatomical and dimensional variations of the nasopalatine canal (NPC) according to sex, age and dental status. Methods: This observational cross-sectional study analyzed a total of 251 CBCT images obtained from the database from the Oral and Maxillofacial Imaging Service of the School of Dentistry of Universidad Andres Bello, Viña del Mar, Chile. Student-t test, chi-square test and ANOVA (p<0.05) were performed to evaluate statistical significance according to sex, age and dental status. This study was approved by the Research and Ethics Committee of the School of Dentistry of Andres Bello University. Results: The NPC showed variability in terms of morphology and dimensions. The most predominant shape of the NPC was cylindrical (65.33%) and the most frequent shape of the curvature of the canal in a sagittal plane was straight inclined (54.98%). The mean length was of 10.92±2.82 mm, being greater in males than females (p<0.01). The most frequent anatomical variation in the coronal plane was the single canal type (57.76%). The effect of gender on the distance between the anterior wall of the NPC and the vestibular crest of the maxilla was not significant (p<0.05). In addition, a significant difference was detected between the dental status and the dimension of the vestibular table in relation to the CNP (p<0.01). Conclusion: The use of CBCT revealed the importance of 3D imaging in terms of morpho-dimensional parameters. NCP variations should be considered to avoid possible complications during surgical procedures. [ABSTRACT FROM AUTHOR]