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9 results on '"VINATZER B.A."'

7. Isolation of two microsatellite markers from BAC clones of the Vf scab resistance region and molecular characterization of scab-resistant accessions in Malus germplasm.

Author

Vinatzer, B.A., Patocchi, A., Tartarini, S., Gianfranceschi, L., Sansavini, S., and Gessler, C.

Subjects
*APPLES, *BACTERIAL artificial chromosomes, *MICROSATELLITE repeats, *MOLECULAR cloning, *PLANT germplasm, *POLYMERASE chain reaction
Abstract

A polymerase chain reaction (PCR)-based method was developed to isolate microsatellite markers from large-insert genomic DNA clones of bacterial artificial chromosome (BAC) libraries. The method is fast and economic since it does not require subcloning. It was applied to isolate a microsatellite marker from a BAC clone of the chromosomal region containing the apple scab resistance gene Vf. The Vf gene of Malus floribunda 821 is the most widely used source of scab resistance in apple breeding. A second microsatellite was found on the extremity of a BAC clone flanking the Vf locus. The two microsatellites allowed the identification of the presence of the Vf gene in the scab-resistant accessions M. micromalus SA573-3, ‘Golden Gem’, M. prunifolia 19651 and MA 16 not previously known to carry this gene. They were also used to verify the correctness of the published genealogical tree of the Vf cultivar ‘Florina’, in which a probable mistake was identified. This analysis shows the importance of genotyping the Vf locus when choosing scab-resistant germplasm as parents in breeding programmes. [ABSTRACT FROM AUTHOR]

Published
2004
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8. GALA APPLE TRANSFORMED WITH THE PUTATIVE RESISTANCE GENE HCRVF2

Author

BELFANTI E., BARBIERI M., VINATZER B. A., GENNARI F., SILFVERBERG DILWORTH E., PATOCCHI A., HERMANN D., GIANFRANCESCHI L., GESSLER C., TARTARINI, STEFANO, PARIS, ROBERTA, SANSAVINI, SILVIERO, F. LAURENS, K. EVANS, BELFANTI E., BARBIERI M., TARTARINI S., VINATZER B.A., GENNARI F., PARIS R., SANSAVINI S., SILFVERBERG-DILWORTH E., PATOCCHI A., HERMANN D., GIANFRANCESCHI L., and GESSLER C.

Subjects
fungi, APPLE, SCAB RESISTANCE
Abstract

The HcrVf2 gene recently cloned from a wild Malus species was successfully introgessed into a popular apple cultivar susceptibler to scab. The results reveal that this gene is able to confer scab resistance to a susceptible genotype.

Published
2004

9. TRASFORMAZIONE GENETICA DEL MELO GALA CON UN GENE DI RESISTENZA A TICCHIOLATURA

Author

SANSAVINI, SILVIERO, TARTARINI, STEFANO, BARBIERI M., BELFANTI E., VINATZER B. A., GESSLER C., SILFVERBERG E., GIANFRANCESCHI L., HERMANN D., PATOCCHI A., SANSAVINI S., BARBIERI M., BELFANTI E., TARTARINI S., VINATZER B.A., GESSLER C., SILFVERBERG E., GIANFRANCESCHI L., HERMANN D., and PATOCCHI A.

Subjects
TICCHIOLATURA, MELO, RESISTENZA
Published
2004

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